Rorug01G0181100
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006635
Physical Location & Seq
Forward (+)
24918596 .. 24919682
1087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0181100.1

Sequence Viewer

Length: 693 bp
ATGGAAACTCATTCCAAGAAAATGGCAGAAGTCAAGCTCTTCAGAACATGGTCTAGCCGTTTTGCTTTGAGAATAGTCTGGGCACTGAAGCTCAAAGATGTCCAATATGATACCATCTTTGAAGATCTCTCAAACAAAAGCCCTCTGCTTCTTCAATACAACCCTGTTCATAAGAAGGTTCCAGTGCTTGTGCACAATGGAAAATCAGTTGCTGAATCACTTGTGATCCTTGAATACATTGAAGAAACATGGAAACAGAACCCTTTGCTATCCGAAGATCCTCACGAAAGAGCCGCTGCACGCTTTTGGGCAAAATTTGGTGATGAAAAGGTATTGCCATCTATTTGGGAAGCCTTCAACAGTGAAGGGAAAGAGCAAGAGGAAGGTATTGTGAAGGCCAAGGAGAACTTGAAGTACCTGGAAGAGGAGCTAAAAGGGAAGAAATTCTTTGGGGGAGAGCATATTGGATTTGCAGATATTGCACTTGGATGGCTTGCAGAACTTGAGAATGTGTTTGAAGAGGTAGCTAGCATGAAAGTGATAGCAGAAGATGAGTTTCCATTGTTATCAGAATGGAAAAGGACTTTTGCAGATGCTCCTATAATCAAAGAGAATTGGCCTCCCAGAGACAAACTTGTCACCAAATATCAGGCTTTTCGAGAGGCCAACCTCTTGAACAAGGCACCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

230

Amino Acids

26.68

Weight (kDa)

5.86

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 10 - 82 8e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 13 - 85 3.6e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 19 - 83 6.3e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 110 - 198 2.3e-10 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
AccB1I GGYRCC 1 cut(s) 682
AciI CCGC 1 cut(s) 294
AclWI GGATC 2 cut(s) 220, 272
AcsI RAATTY 2 cut(s) 314, 443
AcuI CTGAAG 2 cut(s) 25, 107
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 424
AgsI TTSAA 8 cut(s) 122, 155, 233, 242, 358, 412, 518, 676
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 4 cut(s) 37, 91, 430, 527
AluI AGCT 4 cut(s) 37, 91, 430, 527
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 621
Alw44I GTGCAC 1 cut(s) 191
AlwI GGATC 2 cut(s) 220, 272
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 3 cut(s) 396, 617, 663
ApaLI GTGCAC 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 2 cut(s) 314, 443
Asp700I GAANNNNTTC 1 cut(s) 443
AsuHPI GGTGA 2 cut(s) 332, 631
AsuNHI GCTAGC 1 cut(s) 527
BaeGI GKGCMC 2 cut(s) 85, 195
BanI GGYRCC 1 cut(s) 682
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 1 cut(s) 283
BccI CCATC 3 cut(s) 122, 346, 483
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 419
BcoDI GTCTC 1 cut(s) 621
BfaI CTAG 2 cut(s) 54, 528
BglII AGATCT 1 cut(s) 124
BisI GCNGC 2 cut(s) 294, 297
BlsI GCNGC 2 cut(s) 295, 298
Bme1390I CCNGG 1 cut(s) 419
BmiI GGNNCC 2 cut(s) 180, 684
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 583
BmtI GCTAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 182
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 1 cut(s) 424
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 440
BseSI GKGCMC 2 cut(s) 85, 195
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 3 cut(s) 398, 619, 665
BshNI GGYRCC 1 cut(s) 682
BsiHKAI GWGCWC 1 cut(s) 195
BslI CCNNNNNNNGG 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 621
BsnI GGCC 3 cut(s) 398, 619, 665
Bsp1286I GDGCHC 2 cut(s) 85, 195
Bsp143I GATC 3 cut(s) 124, 225, 277
BspACI CCGC 1 cut(s) 294
BspANI GGCC 3 cut(s) 398, 619, 665
BspLI GGNNCC 2 cut(s) 180, 684
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 2 cut(s) 220, 272
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 682
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 3 cut(s) 124, 225, 277
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 3 cut(s) 44, 417, 513
BstAPI GCANNNNNTGC 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 301, 495, 529
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 1 cut(s) 494
BstKTI GATC 3 cut(s) 127, 228, 280
BstMAI GTCTC 1 cut(s) 621
BstMBI GATC 3 cut(s) 124, 225, 277
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 417
BstSLI GKGCMC 2 cut(s) 85, 195
BstV1I GCAGC 1 cut(s) 283
BstX2I RGATCY 2 cut(s) 124, 277
BstXI CCANNNNNNTGG 2 cut(s) 22, 345
BstYI RGATCY 2 cut(s) 124, 277
BsuRI GGCC 3 cut(s) 398, 619, 665
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 3 cut(s) 83, 189, 367
Cac8I GCNNGC 3 cut(s) 301, 495, 529
CaiI CAGNNNCTG 1 cut(s) 212
Csp6I GTAC 1 cut(s) 415
CviAII CATG 3 cut(s) 48, 249, 532
CviQI GTAC 1 cut(s) 415
DpnI GATC 3 cut(s) 126, 227, 279
DpnII GATC 3 cut(s) 124, 225, 277
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 3 cut(s) 44, 417, 513
EarI CTCTTC 3 cut(s) 44, 417, 513
Eco130I CCWWGG 1 cut(s) 399
Eco57I CTGAAG 2 cut(s) 25, 107
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 3 cut(s) 51, 252, 535
FaiI YATR 7 cut(s) 49, 108, 171, 250, 462, 533, 602
FalI AAGNNNNNCTT 4 cut(s) 392, 424, 431, 463
FatI CATG 3 cut(s) 47, 248, 531
Fnu4HI GCNGC 2 cut(s) 294, 297
FokI GGATG 1 cut(s) 501
Fsp4HI GCNGC 2 cut(s) 294, 297
FspBI CTAG 2 cut(s) 54, 528
GluI GCNGC 2 cut(s) 294, 297
HaeIII GGCC 3 cut(s) 398, 619, 665
Hin1II CATG 3 cut(s) 51, 252, 535
HinfI GANTC 1 cut(s) 215
HphI GGTGA 2 cut(s) 332, 631
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 44, 274, 571
Hpy188III TCNNGA 3 cut(s) 284, 659, 673
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 5 cut(s) 169, 359, 364, 377, 388
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 6 cut(s) 193, 299, 473, 482, 497, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
Hsp92II CATG 3 cut(s) 51, 252, 535
Kzo9I GATC 3 cut(s) 124, 225, 277
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 2 cut(s) 427, 601
LpnPI CCDG 7 cut(s) 64, 177, 195, 404, 431, 635, 637
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 1 cut(s) 583
MaeI CTAG 2 cut(s) 54, 528
MaeIII GTNAC 1 cut(s) 637
MalI GATC 3 cut(s) 126, 227, 279
MboI GATC 3 cut(s) 124, 225, 277
MboII GAAGA 9 cut(s) 31, 134, 143, 254, 287, 434, 451, 530, 560
MflI RGATCY 2 cut(s) 124, 277
MhlI GDGCHC 2 cut(s) 85, 195
MluCI AATT 3 cut(s) 314, 443, 613
MnlI CCTC 8 cut(s) 153, 291, 373, 418, 514, 630, 655, 680
MroXI GAANNNNTTC 1 cut(s) 443
MslI CAYNNNNRTG 3 cut(s) 487, 536, 688
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 419
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 3 cut(s) 124, 225, 277
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 3 cut(s) 51, 252, 535
NlaIV GGNNCC 2 cut(s) 180, 684
NmuCI GTSAC 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 2 cut(s) 295, 298
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 180, 684
PsrI GAACNNNNNNTAC 2 cut(s) 398, 430
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 2 cut(s) 124, 277
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 3 cut(s) 487, 536, 688
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 2 cut(s) 294, 297
Sau3AI GATC 3 cut(s) 124, 225, 277
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 2 cut(s) 85, 195
SfaNI GCATC 1 cut(s) 583
SmiMI CAYNNNNRTG 3 cut(s) 487, 536, 688
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 3 cut(s) 314, 443, 613
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 54, 528
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 1 cut(s) 362
TaqI TCGA 1 cut(s) 658
TasI AATT 3 cut(s) 314, 443, 613
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 1 cut(s) 215
TscAI CASTG 3 cut(s) 90, 189, 367
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 1 cut(s) 296
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 158, 339, 548
TspRI CASTG 3 cut(s) 90, 189, 367
VneI GTGCAC 1 cut(s) 191
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 2 cut(s) 314, 443
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 54, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.