AT4G03100

P21-Rho-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005281
Physical Location & Seq
Forward (+)
1374160 .. 1376287
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G03100.1

Sequence Viewer

Length: 1293 bp
ATGACGGGGCTAGTGATGATGACTAAAGGCGGAGGTTGCGGCGGCGGAGGAAAAGGAGGAAGGAGAAAATCAACGGCGGAGGAGGAAGAAGAAGAAGAGCAGAATCAGCAACAACTGTCTCTTGTTGAGTTTCTCTTAACGGCGCTGCGTAAATCTGTGGTTTCTTGCCGTGTAGACAACAGACAAGACGACGGCGGAGTAGGCGGAGGGATTTCGTCCGCCGTTCATCACATGGAGATCGGTTGGCCAACAAATGTTCGACACATCACTCATGTAACATTCGATCGATTCCATGGCTTTCTTGGTCTCCCTCACGAGCTTCAAGTTGAGATCCCATGTCGAGTCCCTAGTGCAAGTGTGAGTGTGTTTGGTGTATCGGCGGAATCGATGCAATGTTCTTATGATGAGAAAGGAAACAGTGTCCCAACGATTCTATTACTTATGCAGGAGAGACTCTATTCTCAACAAGGTCTTAAGGCTGAAGGAATTTTCAGGATAAACCCTGAGAATAGTCAAGAGGAACATGTAAGAGACCAACTAAACAGAGGTATTGTACCTGAGAATATTGATGTGCATTGTTTGGCTGGTCTTATTAAAGCTTGGTTTAGAGAGTTACCAAGTGGAGTGCTTGATGGTCTTTCTCCTGAGGAAGTTCTCAATTGCAACACTGAGGATGAATCCGTTGAACTCATTAAGCAGTTGAAGCCAACTGAGTCTGCTTTGCTTAATTGGGCTGTTGATCTTATGGCTGATGTTGTTGAAGAAGAAGAGTCTAACAAAATGAATGCAAGGAATATAGCCATGGTTTTTGCTCCTAATATGACTCAGATGACAGATCCATTAACGGCTCTTATGCATGCTGTTCAAGTGATGAACTTGCTTAAGACTCTTATCACAAAGACACTAGCTGAACGGGAAGAAAATGCTACCGGATCAGAAGGATATTCTCCATCCCACTCATCCAATTCTCAAACTGATTCTGATTCTGACAATGCACAAGACATGGAAGTCAGCTGTGAATCACAAGCCACAGATTCGGAATGCGGAGAAGAAGAAGAAGTAGAGGAAGTAGAACAGCATCAAGAACATCTCAGCCGCCACTCTACCCACGAAGATGAAACCGATATTGGATCATTATGTTCAATAGAGAAATGCTTCTTGAATCAACTCAACAACAATGCTGCTAGAGTTTCAAACACCAGTATCTCTGAAGACTGGAGTCCTAAAGCATTTCCGCTTGTATCATTCACAGAAAACAAAAGCAACACTTTGAGCTCAAGCACTAGCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

430

Amino Acids

47.3

Weight (kDa)

4.61

Isoelectric Point (pI)

57.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 142 - 276 1.3e-21 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 4 cut(s) 325, 830, 940, 1138
AcoI YGGCCR 1 cut(s) 245
AcsI RAATTY 1 cut(s) 486
AcuI CTGAAG 2 cut(s) 501, 1230
AfaI GTAC 1 cut(s) 555
AflII CTTAAG 2 cut(s) 473, 881
AflIII ACRYGT 1 cut(s) 523
AgsI TTSAA 8 cut(s) 323, 686, 703, 761, 866, 1143, 1162, 1194
AjuI GAANNNNNNNTTGG 2 cut(s) 1110, 1142
AluBI AGCT 5 cut(s) 319, 599, 908, 1014, 1275
AluI AGCT 5 cut(s) 319, 599, 908, 1014, 1275
Alw21I GWGCWC 1 cut(s) 1277
Alw26I GTCTC 4 cut(s) 123, 311, 445, 525
AlwI GGATC 4 cut(s) 325, 830, 940, 1138
AoxI GGCC 1 cut(s) 245
ApeKI GCWGC 2 cut(s) 145, 1181
ApoI RAATTY 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 145
AxyI CCTNAGG 1 cut(s) 645
BaeI ACNNNNGTAYC 2 cut(s) 1186, 1219
BalI TGGCCA 1 cut(s) 247
BanII GRGCYC 1 cut(s) 1277
BauI CACGAG 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 1218
Bbv12I GWGCWC 1 cut(s) 1277
BbvI GCAGC 2 cut(s) 132, 1168
BccI CCATC 2 cut(s) 626, 958
BceAI ACGGC 6 cut(s) 90, 153, 156, 206, 208, 861
BcoDI GTCTC 4 cut(s) 123, 311, 445, 525
BfaI CTAG 5 cut(s) 11, 348, 905, 1185, 1284
BfoI RGCGCY 1 cut(s) 146
BfrI CTTAAG 2 cut(s) 473, 881
BisI GCNGC 5 cut(s) 40, 43, 146, 1096, 1182
BlsI GCNGC 5 cut(s) 41, 44, 147, 1097, 1183
BmsI GCATC 2 cut(s) 378, 1087
BoxI GACNNNNGTC 1 cut(s) 1218
BpiI GAAGAC 1 cut(s) 1218
BpmI CTGGAG 1 cut(s) 1237
BpuEI CTTGAG 1 cut(s) 1261
Bsa29I ATCGAT 2 cut(s) 286, 386
BsaI GGTCTC 2 cut(s) 311, 525
BsaJI CCNNGG 2 cut(s) 292, 801
BsaWI WCCGGW 1 cut(s) 929
Bse1I ACTGG 2 cut(s) 1200, 1220
Bse21I CCTNAGG 1 cut(s) 645
Bse3DI GCAATG 1 cut(s) 398
BseCI ATCGAT 2 cut(s) 286, 386
BseDI CCNNGG 2 cut(s) 292, 801
BseGI GGATG 3 cut(s) 679, 950, 959
BseMI GCAATG 1 cut(s) 398
BseMII CTCAG 7 cut(s) 495, 549, 636, 660, 702, 839, 1105
BseNI ACTGG 2 cut(s) 1200, 1220
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 2 cut(s) 132, 1168
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 1 cut(s) 247
BshVI ATCGAT 2 cut(s) 286, 386
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 1277
BsiSI CCGG 1 cut(s) 930
BslFI GGGAC 2 cut(s) 329, 407
BsmAI GTCTC 4 cut(s) 123, 311, 445, 525
BsmFI GGGAC 2 cut(s) 329, 407
BsmI GAATGC 2 cut(s) 790, 1046
BsnI GGCC 1 cut(s) 247
Bso31I GGTCTC 2 cut(s) 311, 525
Bsp1286I GDGCHC 1 cut(s) 1277
Bsp143I GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
Bsp19I CCATGG 2 cut(s) 292, 801
BspANI GGCC 1 cut(s) 247
BspCNI CTCAG 7 cut(s) 496, 550, 637, 661, 703, 838, 1104
BspDI ATCGAT 2 cut(s) 286, 386
BspPI GGATC 4 cut(s) 325, 830, 940, 1138
BspQI GCTCTTC 1 cut(s) 90
BspTI CTTAAG 2 cut(s) 473, 881
BspTNI GGTCTC 2 cut(s) 311, 525
BsrDI GCAATG 1 cut(s) 398
BsrI ACTGG 2 cut(s) 1200, 1220
BssECI CCNNGG 2 cut(s) 292, 801
BssMI GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
BssSI CACGAG 1 cut(s) 314
BssT1I CCWWGG 2 cut(s) 292, 801
Bst2BI CACGAG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 117, 419
Bst6I CTCTTC 2 cut(s) 90, 762
BstAFI CTTAAG 2 cut(s) 473, 881
BstC8I GCNNGC 1 cut(s) 858
BstDEI CTNAG 7 cut(s) 504, 558, 645, 669, 711, 825, 1091
BstDSI CCRYGG 2 cut(s) 292, 801
BstF5I GGATG 3 cut(s) 679, 950, 959
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 7 cut(s) 240, 286, 333, 742, 838, 935, 1133
BstMAI GTCTC 4 cut(s) 123, 311, 445, 525
BstMBI GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 4 cut(s) 36, 106, 201, 703
BstNSI RCATGY 2 cut(s) 527, 860
BstPAI GACNNNNGTC 1 cut(s) 1218
BstV1I GCAGC 2 cut(s) 132, 1168
BstV2I GAAGAC 1 cut(s) 1218
BstX2I RGATCY 2 cut(s) 330, 835
BstYI RGATCY 2 cut(s) 330, 835
Bsu15I ATCGAT 2 cut(s) 286, 386
Bsu36I CCTNAGG 1 cut(s) 645
BsuRI GGCC 1 cut(s) 247
BsuTUI ATCGAT 2 cut(s) 286, 386
BtgI CCRYGG 2 cut(s) 292, 801
BtsCI GGATG 3 cut(s) 679, 950, 959
BtsIMutI CAGTG 2 cut(s) 424, 666
Cac8I GCNNGC 1 cut(s) 858
CfoI GCGC 1 cut(s) 145
ClaI ATCGAT 2 cut(s) 286, 386
Csp6I GTAC 1 cut(s) 554
CviAII CATG 8 cut(s) 232, 272, 293, 336, 524, 802, 857, 1003
CviQI GTAC 1 cut(s) 554
DdeI CTNAG 7 cut(s) 504, 558, 645, 669, 711, 825, 1091
DpnI GATC 7 cut(s) 239, 285, 332, 741, 837, 934, 1132
DpnII GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
DrdI GACNNNNNNGTC 1 cut(s) 1007
DseDI GACNNNNNNGTC 1 cut(s) 1007
EaeI YGGCCR 1 cut(s) 245
Eam1104I CTCTTC 2 cut(s) 90, 762
EarI CTCTTC 2 cut(s) 90, 762
EciI GGCGGA 7 cut(s) 45, 60, 92, 208, 210, 219, 395
Ecl136II GAGCTC 1 cut(s) 1275
Eco130I CCWWGG 2 cut(s) 292, 801
Eco24I GRGCYC 1 cut(s) 1277
Eco31I GGTCTC 2 cut(s) 311, 525
Eco53kI GAGCTC 1 cut(s) 1275
Eco57I CTGAAG 2 cut(s) 501, 1230
Eco81I CCTNAGG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 1275
EcoT14I CCWWGG 2 cut(s) 292, 801
EcoT22I ATGCAT 1 cut(s) 858
EcoT38I GRGCYC 1 cut(s) 1277
ErhI CCWWGG 2 cut(s) 292, 801
FaeI CATG 8 cut(s) 235, 275, 296, 339, 527, 805, 860, 1006
FalI AAGNNNNNCTT 2 cut(s) 1252, 1284
FaqI GGGAC 2 cut(s) 329, 407
FatI CATG 8 cut(s) 231, 271, 292, 335, 523, 801, 856, 1002
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 5 cut(s) 40, 43, 146, 1096, 1182
FokI GGATG 3 cut(s) 686, 937, 946
FriOI GRGCYC 1 cut(s) 1277
Fsp4HI GCNGC 5 cut(s) 40, 43, 146, 1096, 1182
FspBI CTAG 5 cut(s) 11, 348, 905, 1185, 1284
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 40, 43, 146, 1096, 1182
GsuI CTGGAG 1 cut(s) 1237
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 1 cut(s) 247
HapII CCGG 1 cut(s) 930
HhaI GCGC 1 cut(s) 145
Hin1II CATG 8 cut(s) 235, 275, 296, 339, 527, 805, 860, 1006
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HindIII AAGCTT 1 cut(s) 597
HpaII CCGG 1 cut(s) 930
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 6 cut(s) 828, 937, 982, 988, 1039, 1210
Hpy188III TCNNGA 6 cut(s) 314, 493, 515, 644, 1082, 1159
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 54, 476, 932
HpyCH4III ACNGT 2 cut(s) 117, 419
HpyCH4V TGCA 8 cut(s) 353, 391, 445, 574, 663, 788, 856, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 106, 201, 703
HpyF3I CTNAG 7 cut(s) 504, 558, 645, 669, 711, 825, 1091
Hsp92II CATG 8 cut(s) 235, 275, 296, 339, 527, 805, 860, 1006
HspAI GCGC 1 cut(s) 143
Kzo9I GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
LguI GCTCTTC 1 cut(s) 90
LmnI GCTCC 1 cut(s) 817
LpnPI CCDG 9 cut(s) 431, 478, 516, 570, 570, 657, 943, 1201, 1213
Lsp1109I GCAGC 2 cut(s) 132, 1168
LweI GCATC 2 cut(s) 378, 1087
MaeI CTAG 5 cut(s) 11, 348, 905, 1185, 1284
MaeIII GTNAC 2 cut(s) 274, 612
MalI GATC 7 cut(s) 239, 285, 332, 741, 837, 934, 1132
MboI GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
MfeI CAATTG 1 cut(s) 658
MflI RGATCY 2 cut(s) 330, 835
MhlI GDGCHC 1 cut(s) 1277
MlsI TGGCCA 1 cut(s) 247
MluCI AATT 4 cut(s) 486, 658, 727, 964
MluNI TGGCCA 1 cut(s) 247
MlyI GAGTC 7 cut(s) 351, 447, 722, 779, 817, 880, 1228
Mox20I TGGCCA 1 cut(s) 247
Mph1103I ATGCAT 1 cut(s) 858
MroXI GAANNNNTTC 1 cut(s) 1154
MscI TGGCCA 1 cut(s) 247
MseI TTAA 7 cut(s) 137, 474, 594, 693, 726, 842, 882
MslI CAYNNNNRTG 1 cut(s) 1113
Msp20I TGGCCA 1 cut(s) 247
MspA1I CMGCKG 1 cut(s) 1014
MspCI CTTAAG 2 cut(s) 473, 881
MspI CCGG 1 cut(s) 930
MunI CAATTG 1 cut(s) 658
Mva1269I GAATGC 2 cut(s) 790, 1046
MwoI GCNNNNNNNGC 4 cut(s) 36, 106, 201, 703
NcoI CCATGG 2 cut(s) 292, 801
NdeII GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
NlaIII CATG 8 cut(s) 235, 275, 296, 339, 527, 805, 860, 1006
NsiI ATGCAT 1 cut(s) 858
NspI RCATGY 2 cut(s) 527, 860
PaeI GCATGC 1 cut(s) 860
PciI ACATGT 1 cut(s) 523
PciSI GCTCTTC 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 2 cut(s) 790, 1046
PdmI GAANNNNTTC 1 cut(s) 1154
PkrI GCNGC 5 cut(s) 41, 44, 147, 1097, 1183
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 7 cut(s) 350, 447, 721, 778, 817, 880, 1227
PpsI GAGTC 7 cut(s) 350, 447, 721, 778, 817, 880, 1227
PscI ACATGT 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 1218
Psp124BI GAGCTC 1 cut(s) 1277
PsuI RGATCY 2 cut(s) 330, 835
PvuI CGATCG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 1014
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
RseI CAYNNNNRTG 1 cut(s) 1113
SacI GAGCTC 1 cut(s) 1277
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 7 cut(s) 137, 474, 594, 693, 726, 842, 882
SatI GCNGC 5 cut(s) 40, 43, 146, 1096, 1182
Sau3AI GATC 7 cut(s) 237, 283, 330, 739, 835, 932, 1130
SchI GAGTC 7 cut(s) 351, 447, 722, 779, 817, 880, 1228
SduI GDGCHC 1 cut(s) 1277
SetI ASST 9 cut(s) 37, 321, 472, 550, 559, 601, 910, 1016, 1277
SfaNI GCATC 2 cut(s) 378, 1087
SmiMI CAYNNNNRTG 1 cut(s) 1113
SmlI CTYRAG 3 cut(s) 473, 881, 1276
SmoI CTYRAG 3 cut(s) 473, 881, 1276
SphI GCATGC 1 cut(s) 860
Sse9I AATT 4 cut(s) 486, 658, 727, 964
SspI AATATT 1 cut(s) 565
SspMI CTAG 5 cut(s) 11, 348, 905, 1185, 1284
SstI GAGCTC 1 cut(s) 1277
StyI CCWWGG 2 cut(s) 292, 801
TaaI ACNGT 2 cut(s) 117, 419
TaqI TCGA 5 cut(s) 259, 282, 286, 340, 386
TasI AATT 4 cut(s) 486, 658, 727, 964
TauI GCSGC 3 cut(s) 42, 45, 1098
Tru1I TTAA 7 cut(s) 137, 474, 594, 693, 726, 842, 882
Tru9I TTAA 7 cut(s) 137, 474, 594, 693, 726, 842, 882
TscAI CASTG 2 cut(s) 424, 673
TseI GCWGC 2 cut(s) 145, 1181
TspDTI ATGAA 5 cut(s) 215, 690, 797, 887, 1131
TspGWI ACGGA 1 cut(s) 670
TspRI CASTG 2 cut(s) 424, 673
Vha464I CTTAAG 2 cut(s) 473, 881
XapI RAATTY 1 cut(s) 486
XceI RCATGY 2 cut(s) 527, 860
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 5 cut(s) 11, 348, 905, 1185, 1284
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.