Rh6BG347000

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005281
Physical Location & Seq
Forward (+)
57807006 .. 57809904
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG347000.1

Sequence Viewer

Length: 1548 bp
ATGGAAGAGAAAAGAAGTTGGAACTTCAAAAACCCCAAAACCCTTAAATGCAAATCAAAGCAACCCATCTCCTCTGACCCAATCACATATTCAAACCCTCTCTACTCTTCACTCCCAACTGAAAGCTTCGCTGGGTTTCACAATTCAAACCCAGAATCACAACCCACGCAGATGACGGGGCTCGTAATGGTGACGAGGGGCGGCGGCTGCGGTGGTGGTTGCAAAGAGGGGAAGAAGAGCTCGGAAGAAGAGCAAAACCAGCTCTCTCTGGTGGATTTTCTGTTGGCCGCGGTGAGGAAATCCATGGTGGCCTGCCGCGTGGAGCGAGGCGAGGAGGTGATCTCCGCCGTCAATAATATGGAGATCGGGTGGCCCACGAATGTCCGGCATGTGACGCATGTCACGTTCGACCGGTTCAACGGGTTTCTGGGTCTTCCGGTGGAGTTCGAGGTCGATGTCCCGGGTCGGGTTCCGAGTGCTAGTGCAAGTGTGTTTGGGGTCTCAGCGGAGTCAATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTCCGTATAAACCCAGAGAACAGCAAAGAGGAGTTTGTGAGAAACCAGTTGAACAGAGGCATTGTGCCTGATGACATTGACGTCCATTGCTTGGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCAGGAGTGCTAGATGGACTATCTCCTGAAGAAGTTCTTCAGTGCAACACAGAAGAGGAATCCTTTGAGCTTGTGAAGCAGTTAAAGCCAACCGAGGCTGCATTACTCAATTGGGCTATCGGTCTGATGGCTGATGTTGTTGAGGAAGAAGAATTCAACAAAATGAATGCTAGAAATATTGCTATGGTTTTTGCTCCAAACATGACTCAGATGTCTGATCCACTAACGGCTTTGATGCATGCGGTTCAAGTAATGAATTTGCTTAAGACCCTAATTATGAAAACACTAAGGGAACGTGAAGAGACTGATGAAACTGGAGGATATTCACCCATGTCATCTTGTTTATCTGATCATCAATCAGACGAAGACATGGATACCAATGGAGATTTGTATAGTCATCCAGACATGGATACCAACTCTGAATTGAGAGGACCAACATCAGATTATGATGAAAATGCCCACTATAGCAACAGCAGTGAAGATGAAGATGAAGTTCGGCCACTCAGTGACATAGAAGAATGCTTCTTAAGACGATTGGATGAGAAGAAAACAGCCCCAAATAGCATGGCAATGGTGAATAAACCAACAAGTAAGTCACTTGGTGAGTATCCGAGTCCTCAAAGTTGCTCGAGCTTTAACATGGAATCTGGCACATCTTCTGTTGATAGCAAAAATGGGAATTCTGGCTTGTGTGCAAGTTATGGGGAAGACTCTGGAAAATGCACTGATATGGAGAGCCCTTCAGTATCATGCTCAACCTTGAAGGAAATGGATATGGAGGACAGAGTGATGGAGTCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

515

Amino Acids

56.87

Weight (kDa)

4.62

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 184 - 318 7e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 695, 955
AatII GACGTC 1 cut(s) 699
AccB7I CCANNNNNTGG 1 cut(s) 706
AccII CGCG 2 cut(s) 290, 318
AciI CCGC 9 cut(s) 201, 204, 210, 288, 290, 316, 345, 506, 986
AclWI GGATC 1 cut(s) 956
AcoI YGGCCR 2 cut(s) 285, 1241
AcsI RAATTY 3 cut(s) 896, 1000, 1423
AcuI CTGAAG 4 cut(s) 729, 767, 792, 1470
AcyI GRCGYC 1 cut(s) 696
AdeI CACNNNGTG 2 cut(s) 1250, 1346
AfiI CCNNNNNNNGG 3 cut(s) 294, 466, 706
AflII CTTAAG 2 cut(s) 1007, 1270
AgeI ACCGGT 1 cut(s) 411
AjnI CCWGG 1 cut(s) 725
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AluBI AGCT 5 cut(s) 126, 240, 262, 814, 1377
AluI AGCT 5 cut(s) 126, 240, 262, 814, 1377
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 2 cut(s) 505, 1040
AlwI GGATC 1 cut(s) 956
Ama87I CYCGRG 2 cut(s) 460, 1372
AoxI GGCC 5 cut(s) 285, 309, 371, 712, 1241
ApeKI GCWGC 2 cut(s) 207, 842
ApoI RAATTY 3 cut(s) 896, 1000, 1423
AsiGI ACCGGT 1 cut(s) 411
Asp700I GAANNNNTTC 2 cut(s) 777, 780
AspS9I GGNCC 2 cut(s) 372, 1175
AsuC2I CCSGG 2 cut(s) 461, 462
AsuHPI GGTGA 6 cut(s) 202, 304, 349, 1062, 1330, 1358
AvaI CYCGRG 2 cut(s) 460, 1372
AvaII GGWCC 1 cut(s) 1175
BanII GRGCYC 3 cut(s) 183, 242, 1484
BbsI GAAGAC 3 cut(s) 425, 1116, 1458
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 2 cut(s) 194, 829
BccI CCATC 4 cut(s) 74, 752, 865, 1528
BceAI ACGGC 2 cut(s) 332, 987
BciT130I CCWGG 1 cut(s) 727
BciVI GTATCC 3 cut(s) 1111, 1147, 1362
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 2 cut(s) 461, 462
BcoDI GTCTC 2 cut(s) 505, 1040
BfaI CTAG 3 cut(s) 480, 755, 915
BfmI CTRYAG 1 cut(s) 1207
BfrI CTTAAG 2 cut(s) 1007, 1270
BfuI GTATCC 3 cut(s) 1111, 1147, 1362
BisI GCNGC 6 cut(s) 202, 205, 208, 288, 316, 843
BlsI GCNGC 6 cut(s) 203, 206, 209, 289, 317, 844
Bme1390I CCNGG 3 cut(s) 461, 462, 727
Bme18I GGWCC 1 cut(s) 1175
BmeT110I CYCGRG 2 cut(s) 460, 1372
BmgT120I GGNCC 2 cut(s) 372, 1175
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 3 cut(s) 461, 462, 727
BmsI GCATC 2 cut(s) 558, 969
BoxI GACNNNNGTC 1 cut(s) 398
BpiI GAAGAC 3 cut(s) 425, 1116, 1458
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 1080
BpuMI CCSGG 2 cut(s) 461, 462
BsaHI GRCGYC 1 cut(s) 696
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 4 cut(s) 288, 303, 460, 837
BsaWI WCCGGW 2 cut(s) 411, 436
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 294, 466, 706
Bse118I RCCGGY 1 cut(s) 411
Bse1I ACTGG 2 cut(s) 661, 1063
Bse3DI GCAATG 2 cut(s) 700, 1320
BseBI CCWGG 1 cut(s) 727
BseDI CCNNGG 4 cut(s) 288, 303, 460, 837
BseGI GGATG 2 cut(s) 1141, 1288
BseLI CCNNNNNNNGG 3 cut(s) 294, 466, 706
BseMI GCAATG 2 cut(s) 700, 1320
BseMII CTCAG 3 cut(s) 516, 965, 1261
BseNI ACTGG 2 cut(s) 661, 1063
BseRI GAGGAG 3 cut(s) 61, 347, 659
BseXI GCAGC 2 cut(s) 194, 829
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 2 cut(s) 290, 318
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 5 cut(s) 287, 311, 373, 714, 1243
BshTI ACCGGT 1 cut(s) 411
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 242
BsiHKCI CYCGRG 2 cut(s) 460, 1372
BsiSI CCGG 4 cut(s) 385, 412, 437, 461
BslFI GGGAC 2 cut(s) 443, 533
BslI CCNNNNNNNGG 3 cut(s) 294, 466, 706
BsmAI GTCTC 2 cut(s) 505, 1040
BsmFI GGGAC 2 cut(s) 443, 533
BsmI GAATGC 2 cut(s) 916, 1268
BsnI GGCC 5 cut(s) 287, 311, 373, 714, 1243
Bso31I GGTCTC 1 cut(s) 505
BsoBI CYCGRG 2 cut(s) 460, 1372
Bsp1286I GDGCHC 3 cut(s) 183, 242, 1484
Bsp143I GATC 4 cut(s) 339, 363, 961, 1093
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 9 cut(s) 201, 204, 210, 288, 290, 316, 345, 506, 986
BspANI GGCC 5 cut(s) 287, 311, 373, 714, 1243
BspCNI CTCAG 3 cut(s) 515, 964, 1260
BspFNI CGCG 2 cut(s) 290, 318
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 1 cut(s) 956
BspQI GCTCTTC 2 cut(s) 230, 243
BspTI CTTAAG 2 cut(s) 1007, 1270
BspTNI GGTCTC 1 cut(s) 505
BsrDI GCAATG 2 cut(s) 700, 1320
BsrFI RCCGGY 1 cut(s) 411
BsrI ACTGG 2 cut(s) 661, 1063
BssAI RCCGGY 1 cut(s) 411
BssECI CCNNGG 4 cut(s) 288, 303, 460, 837
BssMI GATC 4 cut(s) 339, 363, 961, 1093
BssNI GRCGYC 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 727
Bst6I CTCTTC 5 cut(s) 112, 230, 243, 792, 1038
BstACI GRCGYC 1 cut(s) 696
BstAFI CTTAAG 2 cut(s) 1007, 1270
BstC8I GCNNGC 3 cut(s) 313, 712, 984
BstDEI CTNAG 4 cut(s) 502, 951, 1031, 1247
BstDSI CCRYGG 2 cut(s) 288, 303
BstF5I GGATG 2 cut(s) 1141, 1288
BstFNI CGCG 2 cut(s) 290, 318
BstKTI GATC 4 cut(s) 342, 366, 964, 1096
BstMAI GTCTC 2 cut(s) 505, 1040
BstMBI GATC 4 cut(s) 339, 363, 961, 1093
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 6 cut(s) 207, 259, 394, 711, 820, 829
BstNI CCWGG 1 cut(s) 727
BstNSI RCATGY 3 cut(s) 392, 401, 986
BstPAI GACNNNNGTC 1 cut(s) 398
BstSCI CCNGG 3 cut(s) 459, 460, 725
BstSFI CTRYAG 1 cut(s) 1207
BstUI CGCG 2 cut(s) 290, 318
BstV1I GCAGC 2 cut(s) 194, 829
BstV2I GAAGAC 3 cut(s) 425, 1116, 1458
BsuI GTATCC 3 cut(s) 1111, 1147, 1362
BsuRI GGCC 5 cut(s) 287, 311, 373, 714, 1243
BtgI CCRYGG 2 cut(s) 288, 303
BtsCI GGATG 2 cut(s) 1141, 1288
BtsI GCAGTG 1 cut(s) 1225
BtsIMutI CAGTG 4 cut(s) 791, 1225, 1255, 1467
Cac8I GCNNGC 3 cut(s) 313, 712, 984
Cfr10I RCCGGY 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 372, 1175
Cfr42I CCGCGG 1 cut(s) 291
Cfr9I CCCGGG 1 cut(s) 460
CseI GACGC 1 cut(s) 403
CspAI ACCGGT 1 cut(s) 411
DdeI CTNAG 4 cut(s) 502, 951, 1031, 1247
DpnI GATC 4 cut(s) 341, 365, 963, 1095
DpnII GATC 4 cut(s) 339, 363, 961, 1093
DraIII CACNNNGTG 2 cut(s) 1250, 1346
DrdI GACNNNNNNGTC 2 cut(s) 695, 955
DseDI GACNNNNNNGTC 2 cut(s) 695, 955
EaeI YGGCCR 2 cut(s) 285, 1241
Eam1104I CTCTTC 5 cut(s) 112, 230, 243, 792, 1038
EarI CTCTTC 5 cut(s) 112, 230, 243, 792, 1038
EciI GGCGGA 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 714
Eco24I GRGCYC 3 cut(s) 183, 242, 1484
Eco31I GGTCTC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 1175
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 4 cut(s) 729, 767, 792, 1470
Eco88I CYCGRG 2 cut(s) 460, 1372
EcoICRI GAGCTC 1 cut(s) 240
EcoRI GAATTC 2 cut(s) 896, 1423
EcoRII CCWGG 1 cut(s) 725
EcoT14I CCWWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 984
EcoT38I GRGCYC 3 cut(s) 183, 242, 1484
ErhI CCWWGG 1 cut(s) 303
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FaqI GGGAC 2 cut(s) 443, 533
FbaI TGATCA 1 cut(s) 1093
Fnu4HI GCNGC 6 cut(s) 202, 205, 208, 288, 316, 843
FokI GGATG 2 cut(s) 1128, 1295
FriOI GRGCYC 3 cut(s) 183, 242, 1484
Fsp4HI GCNGC 6 cut(s) 202, 205, 208, 288, 316, 843
FspBI CTAG 3 cut(s) 480, 755, 915
GluI GCNGC 6 cut(s) 202, 205, 208, 288, 316, 843
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 5 cut(s) 287, 311, 373, 714, 1243
HapII CCGG 4 cut(s) 385, 412, 437, 461
HgaI GACGC 1 cut(s) 403
Hin1I GRCGYC 1 cut(s) 696
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 9 cut(s) 155, 509, 530, 803, 949, 1357, 1388, 1454, 1538
HpaII CCGG 4 cut(s) 385, 412, 437, 461
HphI GGTGA 6 cut(s) 202, 304, 349, 1062, 1330, 1358
Hpy188III TCNNGA 5 cut(s) 734, 747, 770, 1145, 1458
HpyAV CCTTC 5 cut(s) 595, 602, 753, 1494, 1501
HpyCH4IV ACGT 3 cut(s) 404, 696, 1039
HpyF10VI GCNNNNNNNGC 6 cut(s) 207, 259, 394, 711, 820, 829
HpyF3I CTNAG 4 cut(s) 502, 951, 1031, 1247
HpySE526I ACGT 3 cut(s) 404, 696, 1039
Hsp92I GRCGYC 1 cut(s) 696
Ksp22I TGATCA 1 cut(s) 1093
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 4 cut(s) 339, 363, 961, 1093
LguI GCTCTTC 2 cut(s) 230, 243
LmnI GCTCC 2 cut(s) 322, 943
Lsp1109I GCAGC 2 cut(s) 194, 829
LweI GCATC 2 cut(s) 558, 969
MaeI CTAG 3 cut(s) 480, 755, 915
MaeII ACGT 3 cut(s) 404, 696, 1039
MaeIII GTNAC 5 cut(s) 190, 391, 400, 1250, 1338
MalI GATC 4 cut(s) 341, 365, 963, 1095
MboI GATC 4 cut(s) 339, 363, 961, 1093
MfeI CAATTG 1 cut(s) 853
MhlI GDGCHC 3 cut(s) 183, 242, 1484
MluCI AATT 7 cut(s) 142, 853, 896, 1000, 1017, 1166, 1423
MlyI GAGTC 5 cut(s) 518, 943, 1366, 1448, 1547
Mph1103I ATGCAT 1 cut(s) 984
MroXI GAANNNNTTC 2 cut(s) 777, 780
MseI TTAA 5 cut(s) 45, 827, 1008, 1271, 1380
MslI CAYNNNNRTG 3 cut(s) 170, 1313, 1472
MspA1I CMGCKG 2 cut(s) 290, 506
MspCI CTTAAG 2 cut(s) 1007, 1270
MspI CCGG 4 cut(s) 385, 412, 437, 461
MspR9I CCNGG 3 cut(s) 461, 462, 727
MunI CAATTG 1 cut(s) 853
Mva1269I GAATGC 2 cut(s) 916, 1268
MvaI CCWGG 1 cut(s) 727
MvnI CGCG 2 cut(s) 290, 318
MwoI GCNNNNNNNGC 6 cut(s) 207, 259, 394, 711, 820, 829
NciI CCSGG 2 cut(s) 461, 462
NcoI CCATGG 1 cut(s) 303
NdeII GATC 4 cut(s) 339, 363, 961, 1093
NlaIV GGNNCC 1 cut(s) 471
NmuCI GTSAC 5 cut(s) 190, 391, 400, 1250, 1338
NsiI ATGCAT 1 cut(s) 984
NspI RCATGY 3 cut(s) 392, 401, 986
PaeI GCATGC 1 cut(s) 986
PaeR7I CTCGAG 1 cut(s) 1372
PceI AGGCCT 1 cut(s) 714
PciSI GCTCTTC 2 cut(s) 230, 243
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 2 cut(s) 916, 1268
PdmI GAANNNNTTC 2 cut(s) 777, 780
PfeI GAWTC 4 cut(s) 155, 530, 803, 1388
PflMI CCANNNNNTGG 1 cut(s) 706
PinAI ACCGGT 1 cut(s) 411
PkrI GCNGC 6 cut(s) 203, 206, 209, 289, 317, 844
PleI GAGTC 5 cut(s) 517, 943, 1365, 1448, 1546
PpsI GAGTC 5 cut(s) 517, 943, 1365, 1448, 1546
PshAI GACNNNNGTC 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 725
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 725
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 2 cut(s) 372, 1175
PspXI VCTCGAGB 1 cut(s) 1372
RseI CAYNNNNRTG 3 cut(s) 170, 1313, 1472
SacI GAGCTC 1 cut(s) 242
SacII CCGCGG 1 cut(s) 291
SapI GCTCTTC 2 cut(s) 230, 243
SaqAI TTAA 5 cut(s) 45, 827, 1008, 1271, 1380
SatI GCNGC 6 cut(s) 202, 205, 208, 288, 316, 843
Sau3AI GATC 4 cut(s) 339, 363, 961, 1093
Sau96I GGNCC 2 cut(s) 372, 1175
SchI GAGTC 5 cut(s) 518, 943, 1366, 1448, 1547
ScrFI CCNGG 3 cut(s) 461, 462, 727
SduI GDGCHC 3 cut(s) 183, 242, 1484
SfaNI GCATC 2 cut(s) 558, 969
SfcI CTRYAG 1 cut(s) 1207
Sfr274I CTCGAG 1 cut(s) 1372
Sfr303I CCGCGG 1 cut(s) 291
SgrBI CCGCGG 1 cut(s) 291
SinI GGWCC 1 cut(s) 1175
SlaI CTCGAG 1 cut(s) 1372
SmaI CCCGGG 1 cut(s) 462
SmiMI CAYNNNNRTG 3 cut(s) 170, 1313, 1472
SmlI CTYRAG 3 cut(s) 1007, 1270, 1372
SmoI CTYRAG 3 cut(s) 1007, 1270, 1372
SphI GCATGC 1 cut(s) 986
Sse9I AATT 7 cut(s) 142, 853, 896, 1000, 1017, 1166, 1423
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 9 cut(s) 201, 204, 210, 288, 290, 316, 345, 506, 986
SspI AATATT 2 cut(s) 615, 922
SspMI CTAG 3 cut(s) 480, 755, 915
SstI GAGCTC 1 cut(s) 242
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 3 cut(s) 459, 460, 725
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 3 cut(s) 407, 699, 1042
TaqI TCGA 5 cut(s) 408, 447, 453, 733, 1373
TaqII GACCGA 1 cut(s) 854
TasI AATT 7 cut(s) 142, 853, 896, 1000, 1017, 1166, 1423
TauI GCSGC 4 cut(s) 204, 207, 290, 318
TfiI GAWTC 4 cut(s) 155, 530, 803, 1388
Tru1I TTAA 5 cut(s) 45, 827, 1008, 1271, 1380
Tru9I TTAA 5 cut(s) 45, 827, 1008, 1271, 1380
TscAI CASTG 4 cut(s) 791, 1225, 1255, 1474
TseFI GTSAC 5 cut(s) 190, 391, 400, 1250, 1338
TseI GCWGC 2 cut(s) 207, 842
Tsp45I GTSAC 5 cut(s) 190, 391, 400, 1250, 1338
TspDTI ATGAA 7 cut(s) 923, 1013, 1037, 1068, 1209, 1242, 1248
TspGWI ACGGA 1 cut(s) 608
TspMI CCCGGG 1 cut(s) 460
TspRI CASTG 4 cut(s) 791, 1225, 1255, 1474
Van91I CCANNNNNTGG 1 cut(s) 706
Vha464I CTTAAG 2 cut(s) 1007, 1270
VpaK11BI GGWCC 1 cut(s) 1175
XapI RAATTY 3 cut(s) 896, 1000, 1423
XceI RCATGY 3 cut(s) 392, 401, 986
XhoI CTCGAG 1 cut(s) 1372
XmaI CCCGGG 1 cut(s) 460
XmnI GAANNNNTTC 2 cut(s) 777, 780
XspI CTAG 3 cut(s) 480, 755, 915
ZraI GACGTC 1 cut(s) 697
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.