FvH4_2g24611

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005281
Physical Location & Seq
Forward (+)
20042357 .. 20044331
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24611.t1

Sequence Viewer

Length: 1419 bp
ATGACTGGGCTTGTGATGGTGACGAGGGGAGGAGGGTGCGGTGGTAATTTCAAGGGGGTCAAAGGAGGGTCAACGAAGGAGGAGGAGCAGAACCAGCTCTCGCTGGTGGACTTTCTATTGTCTGCTATAAGGAAATCCATGGTGGCTTGCCGGGTTGAGCAAGCTGAGGATATGATCTCGGCCGTCCAGAACTTGGAGATCGGGTGGCCCACCAATGTCCGGCACATTACGCATGTCACGTTCGACCGGTTCAATGGGTTTCTGGGACTTCCGGTTGAGTTTGAGGTTGAGATTCCTGGTCGTGTTCCAAGTGCCAGTGCAAGTGTGTTTGGAGTCTCAGTTGATTCAATGCAGCTGTCTTTTGATTCAAAAGGGAATAGCGTGCCAACCATTCTGCTTCTAATGCAGGAGAGGTTATATGCACAAGGAGGATTGAAGGCTGAGGGAATATTTCGTATAAACCCAGAGAATGGGCAAGAGGAGATTGTGAGAGGCCAATTGAACAGGGGCATTGTGCCAGATGACGTTGATGTTCATTGTCTGTCTGGTCTGATTAAGGCCTGGTTTCGAGAGCTTCCTTCGGGTGTGCTAGATGGTCTATCCCCTGAACATGTTCTTCAGTGTGAGACAGAAGAGGACTGTGTTGAGCTTGTTAAGCAGTTAAAGCCAACTGAGGCTGCATTGCTCAATTGGGCCATTGATCTGATGGCTGATGTTGTTGAGGAAGAAGAATTCAACAAGATGAATGCAAGAAATATTGCTATGGTTTTCGCTCCAAACATGACTCAGATGTCTGATCCATTGACGGCTTTGATGCATGCTGTTCAAGTAATGAATTTGCTCAAGACCCTGATTACGAGAACACTTAATGAGCGGGAAGAGACTGAGACCGGACCATATTCACAATCACCTATGTCATCTTGTTCATCTGAGGGTCAAACAGATGAAGACTTTGATAGTCAGCAAGAGGTTGAAAGCAGCTCCGAGGTAATAAAGACTTCACCAACATCAGACTATGATGAAAATGGAAGCAGCAGCAACAACAGTGAAGATGAATCTGAAGTCAAGTTACTGAATGACATAGAAGAATGCTTACGGCAATTGGATGAGAATAAACTGTCGACAAATAACTTGGCAATGTTGGGCGAACTGACTAGCGAGTTGCAGGGTGAGTGTTTCAGTCCTCAGAGTTGCTCCGGCTTCAATGTGGAGTCTGCCACATCCTTTACAGACAGCAAAAATAGGGATTCTGACTCGAGTACAAGTGACGGGGAAGAAGACTCTGTAAAATCTATCATAGCTGTGGCACAGGACTCTGATATAAAAAACCAGACAAGCACAAATGAAATGGAGCTGGTAAACAGGTCTGTGGAGTCCGTTTTGCCTATGCCATTAGCTGCATCTACTGAATCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

473

Amino Acids

51.57

Weight (kDa)

4.3

Isoelectric Point (pI)

56.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 129 - 263 4.8e-19 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 790
AccB7I CCANNNNNTGG 2 cut(s) 193, 470
AccBSI CCGCTC 1 cut(s) 874
AccI GTMKAC 1 cut(s) 1121
AciI CCGC 2 cut(s) 39, 874
AclWI GGATC 1 cut(s) 791
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 2 cut(s) 731, 835
AcuI CTGAAG 2 cut(s) 602, 1080
AfaI GTAC 1 cut(s) 1261
AfiI CCNNNNNNNGG 3 cut(s) 193, 219, 470
AflIII ACRYGT 1 cut(s) 610
AgeI ACCGGT 1 cut(s) 246
AjnI CCWGG 2 cut(s) 295, 560
AluBI AGCT 9 cut(s) 97, 164, 355, 574, 649, 981, 1301, 1354, 1397
AluI AGCT 9 cut(s) 97, 164, 355, 574, 649, 981, 1301, 1354, 1397
Alw26I GTCTC 4 cut(s) 340, 620, 875, 881
AlwI GGATC 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 1255
AoxI GGCC 5 cut(s) 180, 206, 493, 558, 693
ApeKI GCWGC 6 cut(s) 352, 677, 978, 1032, 1035, 1397
ApoI RAATTY 2 cut(s) 731, 835
AsiGI ACCGGT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 612
AspS9I GGNCC 3 cut(s) 207, 693, 893
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 4 cut(s) 31, 900, 993, 1181
AvaI CYCGRG 1 cut(s) 1255
AvaII GGWCC 1 cut(s) 893
BarI GAAGNNNNNNTAC 4 cut(s) 1053, 1077, 1085, 1109
BbsI GAAGAC 2 cut(s) 954, 1284
BbvCI CCTCAGC 2 cut(s) 165, 441
BbvI GCAGC 6 cut(s) 364, 664, 990, 1044, 1047, 1384
BccI CCATC 3 cut(s) 10, 587, 700
BceAI ACGGC 3 cut(s) 167, 822, 1112
BciT130I CCWGG 2 cut(s) 297, 562
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 4 cut(s) 340, 620, 875, 881
BfaI CTAG 2 cut(s) 590, 1155
BisI GCNGC 6 cut(s) 353, 678, 979, 1033, 1036, 1398
BlsI GCNGC 6 cut(s) 354, 679, 980, 1034, 1037, 1399
Bme1390I CCNGG 3 cut(s) 152, 297, 562
Bme18I GGWCC 1 cut(s) 893
BmeT110I CYCGRG 1 cut(s) 1255
BmgT120I GGNCC 3 cut(s) 207, 693, 893
BmrFI CCNGG 3 cut(s) 152, 297, 562
BmrI ACTGGG 1 cut(s) 15
BmsI GCATC 2 cut(s) 804, 1409
BmuI ACTGGG 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 954, 1284
Bpu10I CCTNAGC 2 cut(s) 165, 441
BpuEI CTTGAG 1 cut(s) 827
BpuMI CCSGG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 881
BsaJI CCNNGG 2 cut(s) 138, 984
BsaWI WCCGGW 3 cut(s) 246, 271, 890
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 324, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 219, 470
Bse118I RCCGGY 1 cut(s) 246
Bse1I ACTGG 2 cut(s) 10, 315
Bse3DI GCAATG 2 cut(s) 680, 1143
BseBI CCWGG 2 cut(s) 297, 562
BseDI CCNNGG 2 cut(s) 138, 984
BseGI GGATG 2 cut(s) 1111, 1220
BseLI CCNNNNNNNGG 3 cut(s) 193, 219, 470
BseMI GCAATG 2 cut(s) 680, 1143
BseMII CTCAG 8 cut(s) 156, 351, 432, 663, 800, 876, 921, 1199
BseNI ACTGG 2 cut(s) 10, 315
BseRI GAGGAG 4 cut(s) 45, 95, 98, 494
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 6 cut(s) 364, 664, 990, 1044, 1047, 1384
Bsh1285I CGRYCG 2 cut(s) 183, 247
BshFI GGCC 5 cut(s) 182, 208, 495, 560, 695
BshTI ACCGGT 1 cut(s) 246
BsiEI CGRYCG 2 cut(s) 183, 247
BsiHKCI CYCGRG 1 cut(s) 1255
BsiSI CCGG 6 cut(s) 151, 220, 247, 272, 891, 1197
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 193, 219, 470
BsmAI GTCTC 4 cut(s) 340, 620, 875, 881
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 2 cut(s) 751, 1094
BsnI GGCC 5 cut(s) 182, 208, 495, 560, 695
Bso31I GGTCTC 1 cut(s) 881
BsoBI CYCGRG 1 cut(s) 1255
Bsp143I GATC 4 cut(s) 174, 198, 700, 796
Bsp19I CCATGG 1 cut(s) 138
BspACI CCGC 2 cut(s) 39, 874
BspANI GGCC 5 cut(s) 182, 208, 495, 560, 695
BspCNI CTCAG 8 cut(s) 157, 350, 433, 664, 799, 877, 922, 1198
BspPI GGATC 1 cut(s) 791
BspTNI GGTCTC 1 cut(s) 881
BsrBI CCGCTC 1 cut(s) 874
BsrDI GCAATG 2 cut(s) 680, 1143
BsrFI RCCGGY 1 cut(s) 246
BsrI ACTGG 2 cut(s) 10, 315
BssAI RCCGGY 1 cut(s) 246
BssECI CCNNGG 2 cut(s) 138, 984
BssMI GATC 4 cut(s) 174, 198, 700, 796
BssT1I CCWWGG 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 297, 562
Bst4CI ACNGT 3 cut(s) 641, 1046, 1119
Bst6I CTCTTC 2 cut(s) 627, 873
BstC8I GCNNGC 4 cut(s) 148, 162, 383, 819
BstDEI CTNAG 8 cut(s) 165, 337, 441, 672, 786, 885, 930, 1185
BstDSI CCRYGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 1111, 1220
BstKTI GATC 4 cut(s) 177, 201, 703, 799
BstMAI GTCTC 4 cut(s) 340, 620, 875, 881
BstMBI GATC 4 cut(s) 174, 198, 700, 796
BstMCI CGRYCG 2 cut(s) 183, 247
BstMWI GCNNNNNNNGC 5 cut(s) 94, 229, 403, 655, 664
BstNI CCWGG 2 cut(s) 297, 562
BstNSI RCATGY 3 cut(s) 236, 614, 821
BstSCI CCNGG 3 cut(s) 150, 295, 560
BstV1I GCAGC 6 cut(s) 364, 664, 990, 1044, 1047, 1384
BstV2I GAAGAC 2 cut(s) 954, 1284
BstZI CGGCCG 1 cut(s) 180
BsuRI GGCC 5 cut(s) 182, 208, 495, 560, 695
BtgI CCRYGG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 1111, 1220
BtsIMutI CAGTG 3 cut(s) 322, 626, 1051
Cac8I GCNNGC 4 cut(s) 148, 162, 383, 819
Cfr10I RCCGGY 1 cut(s) 246
Cfr13I GGNCC 3 cut(s) 207, 693, 893
Csp6I GTAC 1 cut(s) 1260
CspAI ACCGGT 1 cut(s) 246
CviAII CATG 5 cut(s) 139, 233, 611, 781, 818
CviQI GTAC 1 cut(s) 1260
DdeI CTNAG 8 cut(s) 165, 337, 441, 672, 786, 885, 930, 1185
DpnI GATC 4 cut(s) 176, 200, 702, 798
DpnII GATC 4 cut(s) 174, 198, 700, 796
DrdI GACNNNNNNGTC 1 cut(s) 790
DseDI GACNNNNNNGTC 1 cut(s) 790
EaeI YGGCCR 1 cut(s) 180
EagI CGGCCG 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 627, 873
EarI CTCTTC 2 cut(s) 627, 873
EclXI CGGCCG 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 881
Eco47I GGWCC 1 cut(s) 893
Eco52I CGGCCG 1 cut(s) 180
Eco57I CTGAAG 2 cut(s) 602, 1080
Eco88I CYCGRG 1 cut(s) 1255
EcoRI GAATTC 1 cut(s) 731
EcoRII CCWGG 2 cut(s) 295, 560
EcoT14I CCWWGG 1 cut(s) 138
EcoT22I ATGCAT 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 138
FaeI CATG 5 cut(s) 142, 236, 614, 784, 821
FaqI GGGAC 1 cut(s) 279
FatI CATG 5 cut(s) 138, 232, 610, 780, 817
FauI CCCGC 1 cut(s) 867
FblI GTMKAC 1 cut(s) 1121
Fnu4HI GCNGC 6 cut(s) 353, 678, 979, 1033, 1036, 1398
FokI GGATG 2 cut(s) 1118, 1207
Fsp4HI GCNGC 6 cut(s) 353, 678, 979, 1033, 1036, 1398
FspBI CTAG 2 cut(s) 590, 1155
GluI GCNGC 6 cut(s) 353, 678, 979, 1033, 1036, 1398
HaeIII GGCC 5 cut(s) 182, 208, 495, 560, 695
HapII CCGG 6 cut(s) 151, 220, 247, 272, 891, 1197
Hin1II CATG 5 cut(s) 142, 236, 614, 784, 821
HincII GTYRAC 2 cut(s) 72, 1122
HindII GTYRAC 2 cut(s) 72, 1122
HpaII CCGG 6 cut(s) 151, 220, 247, 272, 891, 1197
HphI GGTGA 4 cut(s) 31, 900, 993, 1181
Hpy166II GTNNAC 4 cut(s) 72, 109, 1122, 1360
Hpy188III TCNNGA 3 cut(s) 187, 569, 844
Hpy8I GTNNAC 4 cut(s) 72, 109, 1122, 1360
HpyAV CCTTC 3 cut(s) 70, 430, 588
HpyCH4III ACNGT 3 cut(s) 641, 1046, 1119
HpyCH4IV ACGT 2 cut(s) 239, 525
HpyCH4V TGCA 9 cut(s) 320, 352, 406, 422, 680, 749, 817, 1165, 1400
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 229, 403, 655, 664
HpyF3I CTNAG 8 cut(s) 165, 337, 441, 672, 786, 885, 930, 1185
HpySE526I ACGT 2 cut(s) 239, 525
Hsp92II CATG 5 cut(s) 142, 236, 614, 784, 821
Kzo9I GATC 4 cut(s) 174, 198, 700, 796
LmnI GCTCC 5 cut(s) 85, 778, 986, 1199, 1351
Lsp1109I GCAGC 6 cut(s) 364, 664, 990, 1044, 1047, 1384
LweI GCATC 2 cut(s) 804, 1409
MaeI CTAG 2 cut(s) 590, 1155
MaeII ACGT 2 cut(s) 239, 525
MaeIII GTNAC 4 cut(s) 19, 235, 1068, 1265
MalI GATC 4 cut(s) 176, 200, 702, 798
MbiI CCGCTC 1 cut(s) 874
MboI GATC 4 cut(s) 174, 198, 700, 796
MfeI CAATTG 3 cut(s) 497, 688, 1100
MluCI AATT 6 cut(s) 46, 497, 688, 731, 835, 1100
MlyI GAGTC 7 cut(s) 342, 778, 1220, 1247, 1274, 1307, 1382
Mph1103I ATGCAT 1 cut(s) 819
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 4 cut(s) 555, 654, 662, 867
MslI CAYNNNNRTG 1 cut(s) 1301
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 6 cut(s) 151, 220, 247, 272, 891, 1197
MspR9I CCNGG 3 cut(s) 152, 297, 562
MunI CAATTG 3 cut(s) 497, 688, 1100
Mva1269I GAATGC 2 cut(s) 751, 1094
MvaI CCWGG 2 cut(s) 297, 562
MwoI GCNNNNNNNGC 5 cut(s) 94, 229, 403, 655, 664
NciI CCSGG 1 cut(s) 152
NcoI CCATGG 1 cut(s) 138
NdeII GATC 4 cut(s) 174, 198, 700, 796
NlaIII CATG 5 cut(s) 142, 236, 614, 784, 821
NmeAIII GCCGAG 1 cut(s) 158
NmuCI GTSAC 3 cut(s) 19, 235, 1265
NsiI ATGCAT 1 cut(s) 819
NspI RCATGY 3 cut(s) 236, 614, 821
PaeI GCATGC 1 cut(s) 821
PaeR7I CTCGAG 1 cut(s) 1255
PceI AGGCCT 1 cut(s) 560
PciI ACATGT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 2 cut(s) 751, 1094
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 6 cut(s) 292, 344, 365, 1055, 1247, 1409
PflMI CCANNNNNTGG 2 cut(s) 193, 470
PinAI ACCGGT 1 cut(s) 246
PkrI GCNGC 6 cut(s) 354, 679, 980, 1034, 1037, 1399
PleI GAGTC 7 cut(s) 341, 778, 1219, 1247, 1274, 1307, 1381
PpsI GAGTC 7 cut(s) 341, 778, 1219, 1247, 1274, 1307, 1381
PscI ACATGT 1 cut(s) 610
Psp6I CCWGG 2 cut(s) 295, 560
PspGI CCWGG 2 cut(s) 295, 560
PspPI GGNCC 3 cut(s) 207, 693, 893
PspXI VCTCGAGB 1 cut(s) 1255
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 1 cut(s) 1261
RsaNI GTAC 1 cut(s) 1260
RseI CAYNNNNRTG 1 cut(s) 1301
SalI GTCGAC 1 cut(s) 1120
SaqAI TTAA 4 cut(s) 555, 654, 662, 867
SatI GCNGC 6 cut(s) 353, 678, 979, 1033, 1036, 1398
Sau3AI GATC 4 cut(s) 174, 198, 700, 796
Sau96I GGNCC 3 cut(s) 207, 693, 893
SchI GAGTC 7 cut(s) 342, 778, 1220, 1247, 1274, 1307, 1382
ScrFI CCNGG 3 cut(s) 152, 297, 562
SfaNI GCATC 2 cut(s) 804, 1409
Sfr274I CTCGAG 1 cut(s) 1255
SinI GGWCC 1 cut(s) 893
SlaI CTCGAG 1 cut(s) 1255
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 2 cut(s) 842, 1255
SmoI CTYRAG 2 cut(s) 842, 1255
SphI GCATGC 1 cut(s) 821
Sse9I AATT 6 cut(s) 46, 497, 688, 731, 835, 1100
SseBI AGGCCT 1 cut(s) 560
SsiI CCGC 2 cut(s) 39, 874
SspI AATATT 2 cut(s) 450, 757
SspMI CTAG 2 cut(s) 590, 1155
StuI AGGCCT 1 cut(s) 560
StyD4I CCNGG 3 cut(s) 150, 295, 560
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 641, 1046, 1119
TaiI ACGT 2 cut(s) 242, 528
TaqI TCGA 4 cut(s) 243, 568, 1121, 1256
TasI AATT 6 cut(s) 46, 497, 688, 731, 835, 1100
TatI WGTACW 1 cut(s) 1259
TfiI GAWTC 6 cut(s) 292, 344, 365, 1055, 1247, 1409
Tru1I TTAA 4 cut(s) 555, 654, 662, 867
Tru9I TTAA 4 cut(s) 555, 654, 662, 867
TscAI CASTG 3 cut(s) 322, 626, 1051
TseFI GTSAC 3 cut(s) 19, 235, 1265
TseI GCWGC 6 cut(s) 352, 677, 978, 1032, 1035, 1397
Tsp45I GTSAC 3 cut(s) 19, 235, 1265
TspDTI ATGAA 8 cut(s) 524, 758, 848, 915, 960, 1035, 1068, 1359
TspGWI ACGGA 1 cut(s) 1366
TspRI CASTG 3 cut(s) 322, 626, 1051
Van91I CCANNNNNTGG 2 cut(s) 193, 470
VpaK11BI GGWCC 1 cut(s) 893
XapI RAATTY 2 cut(s) 731, 835
XceI RCATGY 3 cut(s) 236, 614, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XhoI CTCGAG 1 cut(s) 1255
XmiI GTMKAC 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 590, 1155
Zsp2I ATGCAT 1 cut(s) 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.