pycom11g26120

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005281
Physical Location & Seq
Forward (+)
28755473 .. 28758090
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26120.1

Sequence Viewer

Length: 1710 bp
ATGATGTTAACCATTGACTTGAATGAATTCATAGTCAAGATTTGTGTTGCCAAAAAATACCGAAAGTCAAAGTCACCAAACGCTGGAGATAAAGTGAAATTTCAAACGAGTGGCCGTTTGAACTTAGAAAATAAGTTGGAACTTCAAAAACCCTTGCACCATCTCTTCAATGCGATTCAAAGCAAGCCTCAGATTTTCACACTACGACTGAGATTTGTACTCTCTTTCTCTCTACCCCAACAGCAAAGGTCTCAGCATTTCAGAGACACCCAAAAGATTTTACAGTGCAGAGAGAAACAGAGAGAGAGGGAGATGACAGGGCTTGTGATGCTGACGAGGGGCGGCGGCTGTGGAGGCGGAGGTGGCGGAAATGGGAAAGGAGCAAAAGGAGCTAACAAGAGCTCAGAAGAAGAGCAGAACCAGATCTCTGTGGCTGCTCTTCTGTTAGCTGCTGTGAGGAAATCCATGGTGGCCTGCCGTGTCGAAAGAGGCGAAGAAGTCATCTCCGCCGTCCACAACATGGAGATCGGGTGGCCCACGAACGTCCAGCACGTTACCCATGTCACATTCGATCGGTTCAATGGGTTTCTGGGCCTTCCGGTGGAGTTCGAGGTTGAGGTTCCAGGCAGAGTTCCCAGTGCTAGTGCAAGCGTGTTTGGGGTCTCGGCGGAGTCAATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAGGAAGGATTGAAGGCAGAAGGAATATTTCGTATAAACCCCGAGAACAGCCACGAGGAGCTTGTGAGAAGCCAATTGAACAGGGGCATTGTGCCTGATGACATTGATGTCCATTGTTTGGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCGGGAATACTGGATGGACTGTCTCCTGAAGAAGTTCTTCAATGCAACACGGAGGAGGAATCCGTTGAGCTTGTGAAGCAATTAAAGCCAACCGAGACCGCGTTGCTCAACTGGGCAGTCAATCTGATGGCTGATGTTGTTGAGGAAGAAGAACTCAACAAAATGAATGCGCGAAATATCGCTATGGTTTTCGCTCCAAACATGACTCAGATGTCTGATCCTCTGACGGCTTTGATGCACGCTGTTCAAGTAATGAATTTGCTAAAGACACTGATTATGAAGACGCTAAGAGAGCGTGAAGAGAACGATGAAACTGGAGGATATTCACCAATGTCATCTTGTTCATCTGATCATCAAACCGATGAAGACTCTGATGATAGTCAGCAGGAAATGGATATTAGATCTGAATTAGAGGGACCAACATCAGACTATGATGAAAACACGCACTCCATCCACAGCAGCGAAGATGAAGATGAAGTTCGATCAGTAAGTGAGATAGAAGAGTGTTTATTAAGGCACTTAGGGGAGAACAAAACTGCCGGAAACAGCTTGGTGGTGGCAGATCAACCAACAGGCGAGTTGTGCGGGGAACATCTGAGCCCTCAAAGTTGCATGAGCTTCAACATGGACTCTGGCACATCGTTTTGTGACAGCAAAAATGGGAACTCCGCCCTCAGTACTACGAGTGACGGGGAAGCCTCATCAGGGAAATGTATGGCAGCTGAGGAAACTAATGTTCAAATGGAGAGCCCTTCATCAGCATCGAGTTCAAACAGGAATAAAATAGAGACAGTGGACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

570

Amino Acids

62.81

Weight (kDa)

4.97

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 238 - 372 2.4e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 857, 1117
AccB7I CCANNNNNTGG 3 cut(s) 83, 520, 868
AccII CGCG 2 cut(s) 1007, 1078
AciI CCGC 9 cut(s) 342, 345, 357, 366, 507, 668, 1005, 1491, 1575
AclWI GGATC 1 cut(s) 1118
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 3 cut(s) 26, 98, 1162
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 2 cut(s) 219, 1585
AfiI CCNNNNNNNGG 5 cut(s) 83, 520, 601, 868, 1611
AjnI CCWGG 2 cut(s) 622, 887
AluBI AGCT 8 cut(s) 392, 402, 449, 811, 976, 1455, 1524, 1628
AluI AGCT 8 cut(s) 392, 402, 449, 811, 976, 1455, 1524, 1628
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 6 cut(s) 255, 258, 667, 933, 995, 1688
AlwI GGATC 1 cut(s) 1118
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 5 cut(s) 112, 471, 533, 592, 874
ApeKI GCWGC 4 cut(s) 434, 449, 1365, 1625
ApoI RAATTY 3 cut(s) 26, 98, 1162
Asp700I GAANNNNTTC 3 cut(s) 26, 939, 942
AspLEI GCGC 1 cut(s) 1078
AspS9I GGNCC 3 cut(s) 534, 592, 1322
AsuHPI GGTGA 2 cut(s) 66, 1224
AvaI CYCGRG 1 cut(s) 791
AvaII GGWCC 1 cut(s) 1322
BanII GRGCYC 3 cut(s) 404, 1508, 1658
BauI CACGAG 1 cut(s) 803
BbsI GAAGAC 2 cut(s) 1193, 1278
Bbv12I GWGCWC 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 1629
BbvI GCAGC 4 cut(s) 421, 436, 1377, 1637
BccI CCATC 4 cut(s) 168, 914, 1027, 1364
BceAI ACGGC 4 cut(s) 99, 462, 494, 1149
BciT130I CCWGG 2 cut(s) 624, 889
BclI TGATCA 1 cut(s) 1255
BcoDI GTCTC 6 cut(s) 255, 258, 667, 933, 995, 1688
BfaI CTAG 1 cut(s) 642
BglII AGATCT 2 cut(s) 423, 1307
BisI GCNGC 6 cut(s) 343, 346, 435, 450, 1366, 1626
BlsI GCNGC 6 cut(s) 344, 347, 436, 451, 1367, 1627
BmcAI AGTACT 1 cut(s) 1585
Bme1390I CCNGG 2 cut(s) 624, 889
Bme18I GGWCC 1 cut(s) 1322
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 3 cut(s) 534, 592, 1322
BmiI GGNNCC 2 cut(s) 621, 1323
BmrFI CCNGG 2 cut(s) 624, 889
BmrI ACTGGG 2 cut(s) 630, 1027
BmsI GCATC 4 cut(s) 318, 720, 1131, 1676
BmuI ACTGGG 2 cut(s) 630, 1027
BpiI GAAGAC 2 cut(s) 1193, 1278
BpmI CTGGAG 2 cut(s) 105, 1242
Bpu10I CCTNAGC 1 cut(s) 1629
BsaI GGTCTC 3 cut(s) 255, 667, 995
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 6 cut(s) 78, 108, 515, 545, 1337, 1367
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 520, 601, 868, 1611
Bse1I ACTGG 4 cut(s) 636, 921, 1022, 1225
BseBI CCWGG 2 cut(s) 624, 889
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 925, 1356
BseLI CCNNNNNNNGG 5 cut(s) 83, 520, 601, 868, 1611
BseMII CTCAG 8 cut(s) 200, 203, 266, 417, 1127, 1493, 1594, 1620
BseNI ACTGG 4 cut(s) 636, 921, 1022, 1225
BseRI GAGGAG 2 cut(s) 821, 974
BseXI GCAGC 4 cut(s) 421, 436, 1377, 1637
BsgI GTGCAG 1 cut(s) 307
Bsh1236I CGCG 2 cut(s) 1007, 1078
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 5 cut(s) 114, 473, 535, 594, 876
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 2 cut(s) 599, 1446
BslFI GGGAC 2 cut(s) 695, 1335
BslI CCNNNNNNNGG 5 cut(s) 83, 520, 601, 868, 1611
BsmAI GTCTC 6 cut(s) 255, 258, 667, 933, 995, 1688
BsmFI GGGAC 2 cut(s) 695, 1335
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 5 cut(s) 114, 473, 535, 594, 876
Bso31I GGTCTC 3 cut(s) 255, 667, 995
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 3 cut(s) 404, 1508, 1658
Bsp143I GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 9 cut(s) 342, 345, 357, 366, 507, 668, 1005, 1491, 1575
BspANI GGCC 5 cut(s) 114, 473, 535, 594, 876
BspCNI CTCAG 8 cut(s) 201, 202, 265, 416, 1126, 1494, 1593, 1621
BspFNI CGCG 2 cut(s) 1007, 1078
BspLI GGNNCC 2 cut(s) 621, 1323
BspPI GGATC 1 cut(s) 1118
BspQI GCTCTTC 2 cut(s) 405, 444
BspTNI GGTCTC 3 cut(s) 255, 667, 995
BsrI ACTGG 4 cut(s) 636, 921, 1022, 1225
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
BssSI CACGAG 1 cut(s) 803
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 803
Bst2UI CCWGG 2 cut(s) 624, 889
Bst4CI ACNGT 3 cut(s) 285, 927, 1699
Bst6I CTCTTC 5 cut(s) 170, 405, 444, 1200, 1401
BstC8I GCNNGC 5 cut(s) 185, 475, 649, 874, 1146
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 925, 1356
BstFNI CGCG 2 cut(s) 1007, 1078
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 8 cut(s) 426, 528, 574, 1126, 1258, 1310, 1391, 1471
BstMAI GTCTC 6 cut(s) 255, 258, 667, 933, 995, 1688
BstMBI GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 8 cut(s) 328, 354, 363, 389, 982, 991, 1198, 1488
BstNI CCWGG 2 cut(s) 624, 889
BstSCI CCNGG 2 cut(s) 622, 887
BstUI CGCG 2 cut(s) 1007, 1078
BstV1I GCAGC 4 cut(s) 421, 436, 1377, 1637
BstV2I GAAGAC 2 cut(s) 1193, 1278
BstX2I RGATCY 2 cut(s) 423, 1307
BstYI RGATCY 2 cut(s) 423, 1307
BsuRI GGCC 5 cut(s) 114, 473, 535, 594, 876
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 925, 1356
BtsIMutI CAGTG 4 cut(s) 290, 643, 1175, 1704
Cac8I GCNNGC 5 cut(s) 185, 475, 649, 874, 1146
CfoI GCGC 1 cut(s) 1078
Cfr13I GGNCC 3 cut(s) 534, 592, 1322
CseI GACGC 1 cut(s) 1198
Csp6I GTAC 2 cut(s) 218, 1584
CviAII CATG 6 cut(s) 466, 520, 560, 1108, 1519, 1531
CviQI GTAC 2 cut(s) 218, 1584
DpnI GATC 8 cut(s) 425, 527, 573, 1125, 1257, 1309, 1390, 1470
DpnII GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
DrdI GACNNNNNNGTC 2 cut(s) 857, 1117
DseDI GACNNNNNNGTC 2 cut(s) 857, 1117
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 5 cut(s) 170, 405, 444, 1200, 1401
EarI CTCTTC 5 cut(s) 170, 405, 444, 1200, 1401
EciI GGCGGA 5 cut(s) 372, 381, 496, 683, 1564
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 876
Eco24I GRGCYC 3 cut(s) 404, 1508, 1658
Eco31I GGTCTC 3 cut(s) 255, 667, 995
Eco47I GGWCC 1 cut(s) 1322
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 1 cut(s) 954
Eco88I CYCGRG 1 cut(s) 791
EcoICRI GAGCTC 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 622, 887
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 3 cut(s) 404, 1508, 1658
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 6 cut(s) 469, 523, 563, 1111, 1522, 1534
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 2 cut(s) 695, 1335
FatI CATG 6 cut(s) 465, 519, 559, 1107, 1518, 1530
FauI CCCGC 1 cut(s) 1484
FbaI TGATCA 1 cut(s) 1255
Fnu4HI GCNGC 6 cut(s) 343, 346, 435, 450, 1366, 1626
FokI GGATG 2 cut(s) 932, 1343
FriOI GRGCYC 3 cut(s) 404, 1508, 1658
Fsp4HI GCNGC 6 cut(s) 343, 346, 435, 450, 1366, 1626
FspBI CTAG 1 cut(s) 642
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 6 cut(s) 343, 346, 435, 450, 1366, 1626
GsuI CTGGAG 2 cut(s) 105, 1242
HaeIII GGCC 5 cut(s) 114, 473, 535, 594, 876
HapII CCGG 2 cut(s) 599, 1446
HgaI GACGC 1 cut(s) 1198
HhaI GCGC 1 cut(s) 1078
Hin1II CATG 6 cut(s) 469, 523, 563, 1111, 1522, 1534
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 7 cut(s) 175, 671, 692, 965, 1111, 1274, 1535
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 2 cut(s) 599, 1446
HphI GGTGA 2 cut(s) 66, 1224
Hpy166II GTNNAC 3 cut(s) 9, 514, 1702
Hpy188III TCNNGA 4 cut(s) 37, 896, 909, 932
Hpy8I GTNNAC 3 cut(s) 9, 514, 1702
HpyAV CCTTC 6 cut(s) 605, 749, 757, 764, 915, 1668
HpyCH4III ACNGT 3 cut(s) 285, 927, 1699
HpyCH4IV ACGT 2 cut(s) 543, 552
HpyCH4V TGCA 8 cut(s) 157, 288, 647, 679, 733, 951, 1144, 1518
HpyF10VI GCNNNNNNNGC 8 cut(s) 328, 354, 363, 389, 982, 991, 1198, 1488
HpySE526I ACGT 2 cut(s) 543, 552
Hsp92II CATG 6 cut(s) 469, 523, 563, 1111, 1522, 1534
HspAI GCGC 1 cut(s) 1076
Ksp22I TGATCA 1 cut(s) 1255
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
LguI GCTCTTC 2 cut(s) 405, 444
LmnI GCTCC 4 cut(s) 380, 389, 808, 1105
Lsp1109I GCAGC 4 cut(s) 421, 436, 1377, 1637
LweI GCATC 4 cut(s) 318, 720, 1131, 1676
MaeI CTAG 1 cut(s) 642
MaeII ACGT 2 cut(s) 543, 552
MaeIII GTNAC 5 cut(s) 72, 553, 562, 1553, 1592
MalI GATC 8 cut(s) 425, 527, 573, 1125, 1257, 1309, 1390, 1470
MboI GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 2 cut(s) 423, 1307
MhlI GDGCHC 3 cut(s) 404, 1508, 1658
MluCI AATT 6 cut(s) 26, 98, 824, 986, 1162, 1313
MlyI GAGTC 4 cut(s) 680, 1105, 1268, 1529
MmeI TCCRAC 1 cut(s) 117
MroXI GAANNNNTTC 3 cut(s) 26, 939, 942
MseI TTAA 3 cut(s) 8, 989, 1418
MspA1I CMGCKG 1 cut(s) 1628
MspI CCGG 2 cut(s) 599, 1446
MspR9I CCNGG 2 cut(s) 624, 889
MunI CAATTG 1 cut(s) 824
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 2 cut(s) 624, 889
MvnI CGCG 2 cut(s) 1007, 1078
MwoI GCNNNNNNNGC 8 cut(s) 328, 354, 363, 389, 982, 991, 1198, 1488
NcoI CCATGG 1 cut(s) 465
NdeII GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
NlaIII CATG 6 cut(s) 469, 523, 563, 1111, 1522, 1534
NlaIV GGNNCC 2 cut(s) 621, 1323
NmeAIII GCCGAG 1 cut(s) 644
NmuCI GTSAC 4 cut(s) 72, 562, 1553, 1592
PceI AGGCCT 1 cut(s) 876
PciSI GCTCTTC 2 cut(s) 405, 444
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 549
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 3 cut(s) 26, 939, 942
PfeI GAWTC 3 cut(s) 175, 692, 965
PflMI CCANNNNNTGG 3 cut(s) 83, 520, 868
PkrI GCNGC 6 cut(s) 344, 347, 436, 451, 1367, 1627
Ple19I CGATCG 1 cut(s) 574
PleI GAGTC 4 cut(s) 679, 1105, 1268, 1529
PpsI GAGTC 4 cut(s) 679, 1105, 1268, 1529
Psp124BI GAGCTC 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 622, 887
PspGI CCWGG 2 cut(s) 622, 887
PspN4I GGNNCC 2 cut(s) 621, 1323
PspPI GGNCC 3 cut(s) 534, 592, 1322
PsuI RGATCY 2 cut(s) 423, 1307
PvuI CGATCG 1 cut(s) 574
PvuII CAGCTG 1 cut(s) 1628
RsaI GTAC 2 cut(s) 219, 1585
RsaNI GTAC 2 cut(s) 218, 1584
SacI GAGCTC 1 cut(s) 404
SapI GCTCTTC 2 cut(s) 405, 444
SaqAI TTAA 3 cut(s) 8, 989, 1418
SatI GCNGC 6 cut(s) 343, 346, 435, 450, 1366, 1626
Sau3AI GATC 8 cut(s) 423, 525, 571, 1123, 1255, 1307, 1388, 1468
Sau96I GGNCC 3 cut(s) 534, 592, 1322
ScaI AGTACT 1 cut(s) 1585
SchI GAGTC 4 cut(s) 680, 1105, 1268, 1529
ScrFI CCNGG 2 cut(s) 624, 889
SduI GDGCHC 3 cut(s) 404, 1508, 1658
SfaNI GCATC 4 cut(s) 318, 720, 1131, 1676
SinI GGWCC 1 cut(s) 1322
Sse9I AATT 6 cut(s) 26, 98, 824, 986, 1162, 1313
SseBI AGGCCT 1 cut(s) 876
SsiI CCGC 9 cut(s) 342, 345, 357, 366, 507, 668, 1005, 1491, 1575
SspI AATATT 1 cut(s) 777
SspMI CTAG 1 cut(s) 642
SstI GAGCTC 1 cut(s) 404
StuI AGGCCT 1 cut(s) 876
StyD4I CCNGG 2 cut(s) 622, 887
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 285, 927, 1699
TaiI ACGT 2 cut(s) 546, 555
TaqI TCGA 6 cut(s) 483, 570, 609, 895, 1387, 1670
TasI AATT 6 cut(s) 26, 98, 824, 986, 1162, 1313
TatI WGTACW 2 cut(s) 217, 1583
TauI GCSGC 2 cut(s) 345, 348
TfiI GAWTC 3 cut(s) 175, 692, 965
Tru1I TTAA 3 cut(s) 8, 989, 1418
Tru9I TTAA 3 cut(s) 8, 989, 1418
TscAI CASTG 4 cut(s) 290, 643, 1182, 1704
TseFI GTSAC 4 cut(s) 72, 562, 1553, 1592
TseI GCWGC 4 cut(s) 434, 449, 1365, 1625
Tsp45I GTSAC 4 cut(s) 72, 562, 1553, 1592
TspGWI ACGGA 2 cut(s) 958, 971
TspRI CASTG 4 cut(s) 290, 643, 1182, 1704
Van91I CCANNNNNTGG 3 cut(s) 83, 520, 868
VpaK11BI GGWCC 1 cut(s) 1322
XapI RAATTY 3 cut(s) 26, 98, 1162
XmnI GAANNNNTTC 3 cut(s) 26, 939, 942
XspI CTAG 1 cut(s) 642
ZrmI AGTACT 1 cut(s) 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.