FvH4_2g24600

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005281
Physical Location & Seq
Forward (+)
20034085 .. 20036586
2502 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24600.t1

Sequence Viewer

Length: 1611 bp
ATGGAAAAGAGAACAAGTTGGAACTTGAAAAACCCCAAAACCCTTAAATGCAAATCATCAAAGCAACCCTCTGACCCAATCAAATATTTAAACCCACTCTACTCTTCATTCCCACACTCTGAAAGCTTTTCTGGGTTTCCCAATTCAGACCCAGAAACTCCGATGACGGGGATCGTAATGGTGACGAGGGGCGGCGGCTGCGGCGGAGGAGGCGGTGGTGGTGGGTGTAAAGAGGTGAAGAAGAGCGCGGCGGAGGAGCAGAGCCAGGTGTCTCTGGTGGATGTTCTGTTGGCGGCGGTGAGGAAGTCGATGGTGGCTTGCCGTGTGGAGAGAGGTGAGGAGGTGATCTCCGCCGTTAATAATACTATGGAGATCGGGTGGCCGACGAATGTGCAGCATGTGACGCATGTGACGTTTGATCGGTTTAATGGGTTTCTGGGTCTGCCGGTGGAGTTTGAGGTTGAGGTTCCTGGTCGGGTTCCGAGTGCTAGTGCAAATGTGTTTGGCGTCTCAGCAGAGTCAATGCAGTTGTCTTTTGATTCAAATGGAAATAGTGTGCCTACTATTCTCTTGCTGATGCAAGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTCCGTATAAACCCGGAGAACAGCAAAGAGGAGTTTGTGAGAAACCAGTTGAACAGAGGCATTGTGCCTGATGACATTGATGTCCATTGCTTGGCCGGCCTTATCAAAGCCTGGTTTAGAGAACTTCCTTCAGGAGTGCTAGATGGACTATCTCCTGAAGAAGTTCTGCAGTGCAACACAGAAGAGGAATCCTTTGAGCTTGTGAAGCAGTTAAAACCAACTGAGGCTGCATTACTTAATTGGGCTATTGGTCTGATGGCTGATGTTGTTCAGGAAGAAGAACTCAACAAAATGAATGCAAGAAATATTGCTATGGTTTTTGCTCCAAACATGACTCAGATGTCTGATCCACTAACGGCTTTGATGCATGCTGTTCAAGTGATGAATCTGCTTAAGACCCTGATTATGAAAACACTAAGAGAACGTGCAGAGACTGATGAAACTGGAGGGTATTCACCCATGTCATCTTGTTTATCTGATCATCAATCAGAGGAAGACTTGTATAGTCATCCAGACTTGTATAGTCAGCCAGACTTGTATAGTCAGCCAGATATGGATGCCACCTCTGAATTGAGAGGAGGACTAACATCAGATTATGATGAAAATGCCCACTACAGCAACAGCAGTGAAGATGAAGATGAAGTCCGGCCACTCAGTGACATAGAAGAATGCTTCTTAAGACGATTGGATGAGAGGAGAACCACCCCAAATAGCATGGAAATGGTGAATAAACCAACTAGTAAGTCACATGGTGAGTATCCGAGTCCTCAGAGTTGCTCAAGCTTCAACACGGAATCTGGCACATCTTCTGTTGATAGCAAAATTCGGAATTCTGGTTTGTGTGCAAGTTATGGCGAAGACTCTGGGAAATGCACTGATATGGGGAGCCCTTCAGGATCATGCTCAACCTCGAAGGAAATGGATATGGAGGACAGAGTAGTGGAGTCTGTTTCAACTAGACCTAGGCCATTGTTTGCATCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

537

Amino Acids

58.81

Weight (kDa)

4.75

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 187 - 321 2.6e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 704, 964
AccB7I CCANNNNNTGG 1 cut(s) 715
AccII CGCG 1 cut(s) 248
AclWI GGATC 3 cut(s) 179, 965, 1528
AcoI YGGCCR 3 cut(s) 380, 717, 1271
AcsI RAATTY 2 cut(s) 1446, 1453
AcuI CTGAAG 3 cut(s) 738, 801, 1500
AcyI GRCGYC 1 cut(s) 507
AdeI CACNNNGTG 2 cut(s) 1280, 1376
AfiI CCNNNNNNNGG 2 cut(s) 167, 715
AflII CTTAAG 2 cut(s) 1016, 1300
AgsI TTSAA 7 cut(s) 28, 543, 610, 676, 1001, 1411, 1578
AhlI ACTAGT 1 cut(s) 1361
AjnI CCWGG 3 cut(s) 264, 469, 734
AluBI AGCT 3 cut(s) 126, 823, 1407
AluI AGCT 3 cut(s) 126, 823, 1407
Alw26I GTCTC 3 cut(s) 276, 514, 1049
AlwI GGATC 3 cut(s) 179, 965, 1528
AlwNI CAGNNNCTG 1 cut(s) 1058
AoxI GGCC 5 cut(s) 380, 717, 721, 1271, 1589
ApeKI GCWGC 3 cut(s) 198, 394, 851
ApoI RAATTY 2 cut(s) 1446, 1453
Asp700I GAANNNNTTC 1 cut(s) 786
AspA2I CCTAGG 1 cut(s) 1586
AspLEI GCGC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 8 cut(s) 193, 247, 310, 347, 355, 1071, 1360, 1388
AvrII CCTAGG 1 cut(s) 1586
BanII GRGCYC 1 cut(s) 1514
BbsI GAAGAC 2 cut(s) 1125, 1488
BbvI GCAGC 3 cut(s) 185, 406, 838
BccI CCATC 3 cut(s) 304, 761, 874
BceAI ACGGC 3 cut(s) 306, 338, 996
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 3 cut(s) 266, 471, 736
BciVI GTATCC 1 cut(s) 1392
BclI TGATCA 1 cut(s) 1102
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 3 cut(s) 276, 514, 1049
BcuI ACTAGT 1 cut(s) 1361
BfaI CTAG 5 cut(s) 489, 764, 1362, 1581, 1587
BfmI CTRYAG 2 cut(s) 791, 1237
BfrI CTTAAG 2 cut(s) 1016, 1300
BfuI GTATCC 1 cut(s) 1392
BisI GCNGC 8 cut(s) 193, 196, 199, 202, 249, 294, 395, 852
BlnI CCTAGG 1 cut(s) 1586
BlsI GCNGC 8 cut(s) 194, 197, 200, 203, 250, 295, 396, 853
Bme1390I CCNGG 4 cut(s) 266, 471, 638, 736
BmiI GGNNCC 3 cut(s) 468, 480, 1511
BmrFI CCNGG 4 cut(s) 266, 471, 638, 736
BmsI GCATC 3 cut(s) 567, 978, 1171
BpiI GAAGAC 2 cut(s) 1125, 1488
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 1387
BpuMI CCSGG 1 cut(s) 638
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 1586
BsaXI ACNNNNNCTCC 2 cut(s) 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 715
Bse118I RCCGGY 2 cut(s) 445, 719
Bse1I ACTGG 2 cut(s) 670, 1072
Bse3DI GCAATG 1 cut(s) 709
BseBI CCWGG 3 cut(s) 266, 471, 736
BseDI CCNNGG 1 cut(s) 1586
BseGI GGATG 4 cut(s) 286, 1132, 1186, 1318
BseLI CCNNNNNNNGG 2 cut(s) 167, 715
BseMI GCAATG 1 cut(s) 709
BseMII CTCAG 5 cut(s) 525, 837, 974, 1291, 1406
BseNI ACTGG 2 cut(s) 670, 1072
BseRI GAGGAG 6 cut(s) 222, 269, 353, 668, 1215, 1333
BseXI GCAGC 3 cut(s) 185, 406, 838
BsgI GTGCAG 2 cut(s) 413, 1071
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 5 cut(s) 382, 719, 723, 1273, 1591
BsiSI CCGG 4 cut(s) 446, 638, 720, 1270
BslI CCNNNNNNNGG 2 cut(s) 167, 715
BsmAI GTCTC 3 cut(s) 276, 514, 1049
BsmBI CGTCTC 1 cut(s) 514
BsmI GAATGC 2 cut(s) 925, 1298
BsnI GGCC 5 cut(s) 382, 719, 723, 1273, 1591
Bsp1286I GDGCHC 1 cut(s) 1514
Bsp143I GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
BspANI GGCC 5 cut(s) 382, 719, 723, 1273, 1591
BspCNI CTCAG 5 cut(s) 524, 838, 973, 1290, 1405
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 468, 480, 1511
BspMAI CTGCAG 1 cut(s) 795
BspPI GGATC 3 cut(s) 179, 965, 1528
BspQI GCTCTTC 1 cut(s) 236
BspTI CTTAAG 2 cut(s) 1016, 1300
BsrDI GCAATG 1 cut(s) 709
BsrFI RCCGGY 2 cut(s) 445, 719
BsrI ACTGG 2 cut(s) 670, 1072
BssAI RCCGGY 2 cut(s) 445, 719
BssECI CCNNGG 1 cut(s) 1586
BssMI GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 1586
Bst2UI CCWGG 3 cut(s) 266, 471, 736
Bst6I CTCTTC 3 cut(s) 109, 236, 801
BstACI GRCGYC 1 cut(s) 507
BstAFI CTTAAG 2 cut(s) 1016, 1300
BstC8I GCNNGC 3 cut(s) 319, 721, 993
BstDEI CTNAG 6 cut(s) 511, 846, 960, 1040, 1277, 1392
BstF5I GGATG 4 cut(s) 286, 1132, 1186, 1318
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 7 cut(s) 174, 348, 375, 421, 973, 1105, 1523
BstMAI GTCTC 3 cut(s) 276, 514, 1049
BstMBI GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
BstMWI GCNNNNNNNGC 6 cut(s) 198, 201, 210, 403, 720, 829
BstNI CCWGG 3 cut(s) 266, 471, 736
BstNSI RCATGY 3 cut(s) 401, 410, 995
BstSCI CCNGG 4 cut(s) 264, 469, 636, 734
BstSFI CTRYAG 2 cut(s) 791, 1237
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 185, 406, 838
BstV2I GAAGAC 2 cut(s) 1125, 1488
BsuI GTATCC 1 cut(s) 1392
BsuRI GGCC 5 cut(s) 382, 719, 723, 1273, 1591
BtsCI GGATG 4 cut(s) 286, 1132, 1186, 1318
BtsI GCAGTG 2 cut(s) 800, 1255
BtsIMutI CAGTG 4 cut(s) 800, 1255, 1285, 1497
Cac8I GCNNGC 3 cut(s) 319, 721, 993
CaiI CAGNNNCTG 1 cut(s) 1058
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 2 cut(s) 445, 719
CseI GACGC 2 cut(s) 412, 496
CviAII CATG 9 cut(s) 398, 407, 955, 992, 1084, 1339, 1373, 1524, 1608
DdeI CTNAG 6 cut(s) 511, 846, 960, 1040, 1277, 1392
DpnI GATC 7 cut(s) 173, 347, 374, 420, 972, 1104, 1522
DpnII GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
DraI TTTAAA 1 cut(s) 90
DraIII CACNNNGTG 2 cut(s) 1280, 1376
DrdI GACNNNNNNGTC 2 cut(s) 704, 964
DseDI GACNNNNNNGTC 2 cut(s) 704, 964
EaeI YGGCCR 3 cut(s) 380, 717, 1271
Eam1104I CTCTTC 3 cut(s) 109, 236, 801
EarI CTCTTC 3 cut(s) 109, 236, 801
EciI GGCGGA 3 cut(s) 219, 266, 340
Eco130I CCWWGG 1 cut(s) 1586
Eco24I GRGCYC 1 cut(s) 1514
Eco57I CTGAAG 3 cut(s) 738, 801, 1500
EcoRI GAATTC 1 cut(s) 1453
EcoRII CCWGG 3 cut(s) 264, 469, 734
EcoT14I CCWWGG 1 cut(s) 1586
EcoT22I ATGCAT 1 cut(s) 993
EcoT38I GRGCYC 1 cut(s) 1514
ErhI CCWWGG 1 cut(s) 1586
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 9 cut(s) 401, 410, 958, 995, 1087, 1342, 1376, 1527, 1611
FatI CATG 9 cut(s) 397, 406, 954, 991, 1083, 1338, 1372, 1523, 1607
FbaI TGATCA 1 cut(s) 1102
Fnu4HI GCNGC 8 cut(s) 193, 196, 199, 202, 249, 294, 395, 852
FokI GGATG 4 cut(s) 293, 1119, 1193, 1325
FriOI GRGCYC 1 cut(s) 1514
FseI GGCCGGCC 1 cut(s) 723
Fsp4HI GCNGC 8 cut(s) 193, 196, 199, 202, 249, 294, 395, 852
FspBI CTAG 5 cut(s) 489, 764, 1362, 1581, 1587
GlaI GCGC 1 cut(s) 247
GluI GCNGC 8 cut(s) 193, 196, 199, 202, 249, 294, 395, 852
GsuI CTGGAG 1 cut(s) 1089
HaeIII GGCC 5 cut(s) 382, 719, 723, 1273, 1591
HapII CCGG 4 cut(s) 446, 638, 720, 1270
HgaI GACGC 2 cut(s) 412, 496
HhaI GCGC 1 cut(s) 248
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 9 cut(s) 401, 410, 958, 995, 1087, 1342, 1376, 1527, 1611
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HindIII AAGCTT 2 cut(s) 124, 1405
HinfI GANTC 9 cut(s) 518, 539, 812, 958, 1009, 1387, 1418, 1484, 1568
HpaII CCGG 4 cut(s) 446, 638, 720, 1270
HphI GGTGA 8 cut(s) 193, 247, 310, 347, 355, 1071, 1360, 1388
Hpy188III TCNNGA 5 cut(s) 756, 779, 896, 1136, 1518
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 5 cut(s) 604, 611, 762, 1524, 1531
HpyCH4IV ACGT 2 cut(s) 413, 1048
HpyF10VI GCNNNNNNNGC 6 cut(s) 198, 201, 210, 403, 720, 829
HpyF3I CTNAG 6 cut(s) 511, 846, 960, 1040, 1277, 1392
HpySE526I ACGT 2 cut(s) 413, 1048
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 9 cut(s) 401, 410, 958, 995, 1087, 1342, 1376, 1527, 1611
HspAI GCGC 1 cut(s) 246
KroI GCCGGC 1 cut(s) 719
KroNI GCCGGC 1 cut(s) 721
Ksp22I TGATCA 1 cut(s) 1102
Kzo9I GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 3 cut(s) 256, 952, 1509
Lsp1109I GCAGC 3 cut(s) 185, 406, 838
LweI GCATC 3 cut(s) 567, 978, 1171
MaeI CTAG 5 cut(s) 489, 764, 1362, 1581, 1587
MaeII ACGT 2 cut(s) 413, 1048
MaeIII GTNAC 5 cut(s) 181, 400, 409, 1280, 1368
MalI GATC 7 cut(s) 173, 347, 374, 420, 972, 1104, 1522
MboI GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
MhlI GDGCHC 1 cut(s) 1514
MluCI AATT 5 cut(s) 142, 862, 1193, 1446, 1453
MlyI GAGTC 5 cut(s) 527, 952, 1396, 1478, 1577
Mph1103I ATGCAT 1 cut(s) 993
MroNI GCCGGC 1 cut(s) 719
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 8 cut(s) 45, 89, 357, 426, 836, 861, 1017, 1301
MslI CAYNNNNRTG 3 cut(s) 1343, 1502, 1606
MspCI CTTAAG 2 cut(s) 1016, 1300
MspI CCGG 4 cut(s) 446, 638, 720, 1270
MspR9I CCNGG 4 cut(s) 266, 471, 638, 736
Mva1269I GAATGC 2 cut(s) 925, 1298
MvaI CCWGG 3 cut(s) 266, 471, 736
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 6 cut(s) 198, 201, 210, 403, 720, 829
NaeI GCCGGC 1 cut(s) 721
NciI CCSGG 1 cut(s) 638
NdeII GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
NgoMIV GCCGGC 1 cut(s) 719
NlaIII CATG 9 cut(s) 401, 410, 958, 995, 1087, 1342, 1376, 1527, 1611
NlaIV GGNNCC 3 cut(s) 468, 480, 1511
NmuCI GTSAC 5 cut(s) 181, 400, 409, 1280, 1368
NsiI ATGCAT 1 cut(s) 993
NspI RCATGY 3 cut(s) 401, 410, 995
PaeI GCATGC 1 cut(s) 995
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PctI GAATGC 2 cut(s) 925, 1298
PdiI GCCGGC 1 cut(s) 721
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 4 cut(s) 539, 812, 1009, 1418
PflMI CCANNNNNTGG 1 cut(s) 715
PkrI GCNGC 8 cut(s) 194, 197, 200, 203, 250, 295, 396, 853
PleI GAGTC 5 cut(s) 526, 952, 1395, 1478, 1576
PpsI GAGTC 5 cut(s) 526, 952, 1395, 1478, 1576
Psp6I CCWGG 3 cut(s) 264, 469, 734
PspGI CCWGG 3 cut(s) 264, 469, 734
PspN4I GGNNCC 3 cut(s) 468, 480, 1511
PstI CTGCAG 1 cut(s) 795
PstNI CAGNNNCTG 1 cut(s) 1058
RigI GGCCGGCC 1 cut(s) 723
RseI CAYNNNNRTG 3 cut(s) 1343, 1502, 1606
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 8 cut(s) 45, 89, 357, 426, 836, 861, 1017, 1301
SatI GCNGC 8 cut(s) 193, 196, 199, 202, 249, 294, 395, 852
Sau3AI GATC 7 cut(s) 171, 345, 372, 418, 970, 1102, 1520
SchI GAGTC 5 cut(s) 527, 952, 1396, 1478, 1577
ScrFI CCNGG 4 cut(s) 266, 471, 638, 736
SduI GDGCHC 1 cut(s) 1514
SfaNI GCATC 3 cut(s) 567, 978, 1171
SfcI CTRYAG 2 cut(s) 791, 1237
SmiMI CAYNNNNRTG 3 cut(s) 1343, 1502, 1606
SmlI CTYRAG 3 cut(s) 1016, 1300, 1402
SmoI CTYRAG 3 cut(s) 1016, 1300, 1402
SpeI ACTAGT 1 cut(s) 1361
SphI GCATGC 1 cut(s) 995
Sse9I AATT 5 cut(s) 142, 862, 1193, 1446, 1453
SspI AATATT 3 cut(s) 86, 624, 931
SspMI CTAG 5 cut(s) 489, 764, 1362, 1581, 1587
StyD4I CCNGG 4 cut(s) 264, 469, 636, 734
StyI CCWWGG 1 cut(s) 1586
TaiI ACGT 2 cut(s) 416, 1051
TaqI TCGA 2 cut(s) 308, 1535
TasI AATT 5 cut(s) 142, 862, 1193, 1446, 1453
TauI GCSGC 5 cut(s) 195, 198, 204, 251, 296
TfiI GAWTC 4 cut(s) 539, 812, 1009, 1418
Tru1I TTAA 8 cut(s) 45, 89, 357, 426, 836, 861, 1017, 1301
Tru9I TTAA 8 cut(s) 45, 89, 357, 426, 836, 861, 1017, 1301
TscAI CASTG 4 cut(s) 800, 1255, 1285, 1504
TseFI GTSAC 5 cut(s) 181, 400, 409, 1280, 1368
TseI GCWGC 3 cut(s) 198, 394, 851
Tsp45I GTSAC 5 cut(s) 181, 400, 409, 1280, 1368
TspDTI ATGAA 8 cut(s) 96, 932, 1022, 1046, 1077, 1239, 1272, 1278
TspGWI ACGGA 2 cut(s) 617, 1430
TspRI CASTG 4 cut(s) 800, 1255, 1285, 1504
Van91I CCANNNNNTGG 1 cut(s) 715
Vha464I CTTAAG 2 cut(s) 1016, 1300
XapI RAATTY 2 cut(s) 1446, 1453
XceI RCATGY 3 cut(s) 401, 410, 995
XmaJI CCTAGG 1 cut(s) 1586
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 5 cut(s) 489, 764, 1362, 1581, 1587
Zsp2I ATGCAT 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.