Rh6DG339800

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005281
Physical Location & Seq
Forward (+)
54350458 .. 54352655
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG339800.1

Sequence Viewer

Length: 1434 bp
ATGACGGGGCTCGTAATGGTGACGAGGGGCGGCGGCTGCGGCGGCGGCGGTGGTGGTTGCAAAGAGGGGAAGAAGACCTCGGAAGAAGAGCAAAACCAGCTCTCTCTGGTGGATTTTCTGTTGGCCGCGGTGAGGAAATCCATGGTGGCCTGCCGCGTGGAGCGAGGCGAGGAGGTGATCTCCGCCGTCAATAATATGGAGATCGGGTGGCCCACGAATGTCCGGCATGTGACGCATGTCACGTTCGACCGGTTCAACGGGTTTCTGGGTCTTCCGGTGGAGTTCGAGGTCGATGTCCCGGGTCGGGTTCCGAGTGCTAGTGCAAGTGTGTTTGGGGTCTCAGCGGAGTCAATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTCCGTATAAACCCGGAGAACAGCAAAGAGGAATTTGTGAGAAACCAGTTGAACAGAGGCATTGTGCCTGATGACATTGACGTCCATTGCTTAGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCAGGAGTGCTAGATGGACTATCTCCTGAAGAAGTTCTTCAGTGCAACACAGAAGAGGAATCCTTTGAGCTTGTGAAGCAGTTAAAGCCAACCGAGGCTGCATTACTCAATTGGGCTATCGGTCTGATGGCTGATGTTGTTGAGGAAGAAGAATTCAACAAAATGAATGCAAGAAATATTGCTATGGTTTTTGCTCCAAACATGACTCAGATGTCTGATCCACTAACGGCTTTGATGCATGCGGTTCAAGTAATGAATTTGCTTAAGACCCTGATTATGAAAACACTAAGGGAACGTGAAGAGACTGATGAAACTGGAGGATATTCACCCATGTCATCTTGTTTATCTGATCATCAATCAGACGAAGACTTGTATAGTCATCCAGACATGGATACCAACGGAGACTTGTATAGTCATCCAGACATGGATACCAACTCTGAATTGAGAGGACTAACATCAGATTATGATGAAAATGCCCACTATAGCAACAGCAGTGAAGATGAAGATGAAGTTCGGCCACTCAGTGACATAGAAGAATGCTTCTTAAGACGATTGGATGAGAAGAAAACAGCCCCAAATAGCATGGCAATGGTGAATAAACCAACAAGTAAGTCAAAAGTTGGTGAGTATCCGAGTCCTCAAAGTTGCTCGAGCTTTAACATGGAATCTGGCACATCTTCTGTTGATAGCAAAAATGGGAATTCTGGCTTGTGTGCAAGCTATGGGGAAGACTCTGGAAAATGCACTGATATGGAGAGCCCTTCAGTATCATGCTCAACCTTGAAGGAAATGGATATGGAGGACAGAGTAATGGAGTCTGTTTCAACTAGGCCATTGTTTGCATCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

477

Amino Acids

52.37

Weight (kDa)

4.56

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 130 - 264 6.1e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 533, 793
AatII GACGTC 1 cut(s) 537
AccII CGCG 2 cut(s) 128, 156
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 2 cut(s) 123, 1097
AcsI RAATTY 4 cut(s) 485, 734, 838, 1282
AcuI CTGAAG 4 cut(s) 567, 605, 630, 1329
AcyI GRCGYC 1 cut(s) 534
AdeI CACNNNGTG 1 cut(s) 1106
AfiI CCNNNNNNNGG 2 cut(s) 132, 304
AflII CTTAAG 2 cut(s) 845, 1126
AgeI ACCGGT 1 cut(s) 249
AgsI TTSAA 8 cut(s) 256, 372, 439, 505, 739, 830, 1366, 1407
AjnI CCWGG 1 cut(s) 563
AluBI AGCT 4 cut(s) 100, 652, 1236, 1302
AluI AGCT 4 cut(s) 100, 652, 1236, 1302
Alw26I GTCTC 3 cut(s) 343, 878, 978
AlwI GGATC 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 298, 1231
AoxI GGCC 6 cut(s) 123, 147, 209, 550, 1097, 1412
ApeKI GCWGC 2 cut(s) 36, 680
ApoI RAATTY 4 cut(s) 485, 734, 838, 1282
AsiGI ACCGGT 1 cut(s) 249
Asp700I GAANNNNTTC 2 cut(s) 615, 618
AspS9I GGNCC 1 cut(s) 210
AsuC2I CCSGG 3 cut(s) 299, 300, 467
AsuHPI GGTGA 6 cut(s) 31, 142, 187, 900, 1186, 1217
AvaI CYCGRG 2 cut(s) 298, 1231
BanII GRGCYC 2 cut(s) 12, 1343
BbsI GAAGAC 4 cut(s) 80, 263, 954, 1317
BbvI GCAGC 2 cut(s) 23, 667
BccI CCATC 2 cut(s) 590, 703
BceAI ACGGC 2 cut(s) 170, 825
BciT130I CCWGG 1 cut(s) 565
BciVI GTATCC 3 cut(s) 967, 1003, 1221
BclI TGATCA 1 cut(s) 931
BcnI CCSGG 3 cut(s) 299, 300, 467
BcoDI GTCTC 3 cut(s) 343, 878, 978
BfaI CTAG 3 cut(s) 318, 593, 1410
BfmI CTRYAG 1 cut(s) 1063
BfrI CTTAAG 2 cut(s) 845, 1126
BfuI GTATCC 3 cut(s) 967, 1003, 1221
BisI GCNGC 9 cut(s) 31, 34, 37, 40, 43, 46, 126, 154, 681
BlpI GCTNAGC 1 cut(s) 544
BlsI GCNGC 9 cut(s) 32, 35, 38, 41, 44, 47, 127, 155, 682
Bme1390I CCNGG 4 cut(s) 299, 300, 467, 565
BmeT110I CYCGRG 2 cut(s) 298, 1231
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 4 cut(s) 299, 300, 467, 565
BmsI GCATC 2 cut(s) 396, 807
BoxI GACNNNNGTC 1 cut(s) 236
BpiI GAAGAC 4 cut(s) 80, 263, 954, 1317
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpmI CTGGAG 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 544
BpuMI CCSGG 3 cut(s) 299, 300, 467
BsaHI GRCGYC 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 5 cut(s) 78, 126, 141, 298, 675
BsaWI WCCGGW 2 cut(s) 249, 274
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 304
Bse118I RCCGGY 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 499, 901
Bse3DI GCAATG 2 cut(s) 538, 1176
BseBI CCWGG 1 cut(s) 565
BseDI CCNNGG 5 cut(s) 78, 126, 141, 298, 675
BseGI GGATG 3 cut(s) 961, 997, 1144
BseLI CCNNNNNNNGG 2 cut(s) 132, 304
BseMI GCAATG 2 cut(s) 538, 1176
BseMII CTCAG 3 cut(s) 354, 803, 1117
BseNI ACTGG 2 cut(s) 499, 901
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 23, 667
Bsh1236I CGCG 2 cut(s) 128, 156
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 6 cut(s) 125, 149, 211, 552, 1099, 1414
BshTI ACCGGT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 250
BsiHKCI CYCGRG 2 cut(s) 298, 1231
BsiSI CCGG 5 cut(s) 223, 250, 275, 299, 467
BslFI GGGAC 2 cut(s) 281, 371
BslI CCNNNNNNNGG 2 cut(s) 132, 304
BsmAI GTCTC 3 cut(s) 343, 878, 978
BsmFI GGGAC 2 cut(s) 281, 371
BsmI GAATGC 2 cut(s) 754, 1124
BsnI GGCC 6 cut(s) 125, 149, 211, 552, 1099, 1414
Bso31I GGTCTC 1 cut(s) 343
BsoBI CYCGRG 2 cut(s) 298, 1231
Bsp1286I GDGCHC 2 cut(s) 12, 1343
Bsp143I GATC 4 cut(s) 177, 201, 799, 931
Bsp1720I GCTNAGC 1 cut(s) 544
Bsp19I CCATGG 1 cut(s) 141
BspANI GGCC 6 cut(s) 125, 149, 211, 552, 1099, 1414
BspCNI CTCAG 3 cut(s) 353, 802, 1116
BspFNI CGCG 2 cut(s) 128, 156
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 1 cut(s) 794
BspQI GCTCTTC 1 cut(s) 81
BspTI CTTAAG 2 cut(s) 845, 1126
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 538, 1176
BsrFI RCCGGY 1 cut(s) 249
BsrI ACTGG 2 cut(s) 499, 901
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 5 cut(s) 78, 126, 141, 298, 675
BssMI GATC 4 cut(s) 177, 201, 799, 931
BssNI GRCGYC 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 565
Bst6I CTCTTC 3 cut(s) 81, 630, 876
BstACI GRCGYC 1 cut(s) 534
BstAFI CTTAAG 2 cut(s) 845, 1126
BstC8I GCNNGC 4 cut(s) 151, 550, 822, 1300
BstDEI CTNAG 5 cut(s) 340, 544, 789, 869, 1103
BstDSI CCRYGG 2 cut(s) 126, 141
BstF5I GGATG 3 cut(s) 961, 997, 1144
BstFNI CGCG 2 cut(s) 128, 156
BstKTI GATC 4 cut(s) 180, 204, 802, 934
BstMAI GTCTC 3 cut(s) 343, 878, 978
BstMBI GATC 4 cut(s) 177, 201, 799, 931
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 9 cut(s) 36, 39, 42, 45, 97, 232, 549, 658, 667
BstNI CCWGG 1 cut(s) 565
BstNSI RCATGY 3 cut(s) 230, 239, 824
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 4 cut(s) 297, 298, 465, 563
BstSFI CTRYAG 1 cut(s) 1063
BstUI CGCG 2 cut(s) 128, 156
BstV1I GCAGC 2 cut(s) 23, 667
BstV2I GAAGAC 4 cut(s) 80, 263, 954, 1317
BsuI GTATCC 3 cut(s) 967, 1003, 1221
BsuRI GGCC 6 cut(s) 125, 149, 211, 552, 1099, 1414
BtgI CCRYGG 2 cut(s) 126, 141
BtsCI GGATG 3 cut(s) 961, 997, 1144
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 4 cut(s) 629, 1081, 1111, 1326
Cac8I GCNNGC 4 cut(s) 151, 550, 822, 1300
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 210
Cfr42I CCGCGG 1 cut(s) 129
Cfr9I CCCGGG 1 cut(s) 298
CseI GACGC 1 cut(s) 241
CspAI ACCGGT 1 cut(s) 249
DdeI CTNAG 5 cut(s) 340, 544, 789, 869, 1103
DpnI GATC 4 cut(s) 179, 203, 801, 933
DpnII GATC 4 cut(s) 177, 201, 799, 931
DraIII CACNNNGTG 1 cut(s) 1106
DrdI GACNNNNNNGTC 2 cut(s) 533, 793
DseDI GACNNNNNNGTC 2 cut(s) 533, 793
EaeI YGGCCR 2 cut(s) 123, 1097
Eam1104I CTCTTC 3 cut(s) 81, 630, 876
EarI CTCTTC 3 cut(s) 81, 630, 876
EciI GGCGGA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 141
Eco147I AGGCCT 1 cut(s) 552
Eco24I GRGCYC 2 cut(s) 12, 1343
Eco31I GGTCTC 1 cut(s) 343
Eco57I CTGAAG 4 cut(s) 567, 605, 630, 1329
Eco88I CYCGRG 2 cut(s) 298, 1231
EcoRI GAATTC 2 cut(s) 734, 1282
EcoRII CCWGG 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 822
EcoT38I GRGCYC 2 cut(s) 12, 1343
ErhI CCWWGG 1 cut(s) 141
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FaqI GGGAC 2 cut(s) 281, 371
FbaI TGATCA 1 cut(s) 931
Fnu4HI GCNGC 9 cut(s) 31, 34, 37, 40, 43, 46, 126, 154, 681
FokI GGATG 3 cut(s) 948, 984, 1151
FriOI GRGCYC 2 cut(s) 12, 1343
Fsp4HI GCNGC 9 cut(s) 31, 34, 37, 40, 43, 46, 126, 154, 681
FspBI CTAG 3 cut(s) 318, 593, 1410
GluI GCNGC 9 cut(s) 31, 34, 37, 40, 43, 46, 126, 154, 681
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 6 cut(s) 125, 149, 211, 552, 1099, 1414
HapII CCGG 5 cut(s) 223, 250, 275, 299, 467
HgaI GACGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 534
HinfI GANTC 8 cut(s) 347, 368, 641, 787, 1216, 1247, 1313, 1397
HpaII CCGG 5 cut(s) 223, 250, 275, 299, 467
HphI GGTGA 6 cut(s) 31, 142, 187, 900, 1186, 1217
Hpy188III TCNNGA 6 cut(s) 572, 585, 608, 965, 1001, 1317
HpyAV CCTTC 5 cut(s) 433, 440, 591, 1353, 1360
HpyCH4IV ACGT 3 cut(s) 242, 534, 877
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 39, 42, 45, 97, 232, 549, 658, 667
HpyF3I CTNAG 5 cut(s) 340, 544, 789, 869, 1103
HpySE526I ACGT 3 cut(s) 242, 534, 877
Hsp92I GRCGYC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 931
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 4 cut(s) 177, 201, 799, 931
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 2 cut(s) 160, 781
Lsp1109I GCAGC 2 cut(s) 23, 667
LweI GCATC 2 cut(s) 396, 807
MaeI CTAG 3 cut(s) 318, 593, 1410
MaeII ACGT 3 cut(s) 242, 534, 877
MaeIII GTNAC 4 cut(s) 19, 229, 238, 1106
MalI GATC 4 cut(s) 179, 203, 801, 933
MboI GATC 4 cut(s) 177, 201, 799, 931
MfeI CAATTG 1 cut(s) 691
MhlI GDGCHC 2 cut(s) 12, 1343
MluCI AATT 6 cut(s) 485, 691, 734, 838, 1022, 1282
MlyI GAGTC 5 cut(s) 356, 781, 1225, 1307, 1406
Mph1103I ATGCAT 1 cut(s) 822
MroXI GAANNNNTTC 2 cut(s) 615, 618
MseI TTAA 5 cut(s) 665, 846, 1127, 1239, 1432
MslI CAYNNNNRTG 2 cut(s) 1169, 1331
MspA1I CMGCKG 2 cut(s) 128, 344
MspCI CTTAAG 2 cut(s) 845, 1126
MspI CCGG 5 cut(s) 223, 250, 275, 299, 467
MspR9I CCNGG 4 cut(s) 299, 300, 467, 565
MunI CAATTG 1 cut(s) 691
Mva1269I GAATGC 2 cut(s) 754, 1124
MvaI CCWGG 1 cut(s) 565
MvnI CGCG 2 cut(s) 128, 156
MwoI GCNNNNNNNGC 9 cut(s) 36, 39, 42, 45, 97, 232, 549, 658, 667
NciI CCSGG 3 cut(s) 299, 300, 467
NcoI CCATGG 1 cut(s) 141
NdeII GATC 4 cut(s) 177, 201, 799, 931
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 4 cut(s) 19, 229, 238, 1106
NsiI ATGCAT 1 cut(s) 822
NspI RCATGY 3 cut(s) 230, 239, 824
PaeI GCATGC 1 cut(s) 824
PaeR7I CTCGAG 1 cut(s) 1231
PceI AGGCCT 1 cut(s) 552
PciSI GCTCTTC 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 2 cut(s) 754, 1124
PdmI GAANNNNTTC 2 cut(s) 615, 618
PfeI GAWTC 3 cut(s) 368, 641, 1247
PinAI ACCGGT 1 cut(s) 249
PkrI GCNGC 9 cut(s) 32, 35, 38, 41, 44, 47, 127, 155, 682
PleI GAGTC 5 cut(s) 355, 781, 1224, 1307, 1405
PpsI GAGTC 5 cut(s) 355, 781, 1224, 1307, 1405
PshAI GACNNNNGTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 563
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 1 cut(s) 210
PspXI VCTCGAGB 1 cut(s) 1231
RseI CAYNNNNRTG 2 cut(s) 1169, 1331
SacII CCGCGG 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 665, 846, 1127, 1239, 1432
SatI GCNGC 9 cut(s) 31, 34, 37, 40, 43, 46, 126, 154, 681
Sau3AI GATC 4 cut(s) 177, 201, 799, 931
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 5 cut(s) 356, 781, 1225, 1307, 1406
ScrFI CCNGG 4 cut(s) 299, 300, 467, 565
SduI GDGCHC 2 cut(s) 12, 1343
SfaNI GCATC 2 cut(s) 396, 807
SfcI CTRYAG 1 cut(s) 1063
Sfr274I CTCGAG 1 cut(s) 1231
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 1231
SmaI CCCGGG 1 cut(s) 300
SmiMI CAYNNNNRTG 2 cut(s) 1169, 1331
SmlI CTYRAG 3 cut(s) 845, 1126, 1231
SmoI CTYRAG 3 cut(s) 845, 1126, 1231
SphI GCATGC 1 cut(s) 824
Sse9I AATT 6 cut(s) 485, 691, 734, 838, 1022, 1282
SseBI AGGCCT 1 cut(s) 552
SspI AATATT 2 cut(s) 453, 760
SspMI CTAG 3 cut(s) 318, 593, 1410
StuI AGGCCT 1 cut(s) 552
StyD4I CCNGG 4 cut(s) 297, 298, 465, 563
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 3 cut(s) 245, 537, 880
TaqI TCGA 5 cut(s) 246, 285, 291, 571, 1232
TaqII GACCGA 1 cut(s) 692
TasI AATT 6 cut(s) 485, 691, 734, 838, 1022, 1282
TauI GCSGC 7 cut(s) 33, 36, 42, 45, 48, 128, 156
TfiI GAWTC 3 cut(s) 368, 641, 1247
Tru1I TTAA 5 cut(s) 665, 846, 1127, 1239, 1432
Tru9I TTAA 5 cut(s) 665, 846, 1127, 1239, 1432
TscAI CASTG 4 cut(s) 629, 1081, 1111, 1333
TseFI GTSAC 4 cut(s) 19, 229, 238, 1106
TseI GCWGC 2 cut(s) 36, 680
Tsp45I GTSAC 4 cut(s) 19, 229, 238, 1106
TspDTI ATGAA 7 cut(s) 761, 851, 875, 906, 1065, 1098, 1104
TspGWI ACGGA 2 cut(s) 446, 996
TspMI CCCGGG 1 cut(s) 298
TspRI CASTG 4 cut(s) 629, 1081, 1111, 1333
Vha464I CTTAAG 2 cut(s) 845, 1126
XapI RAATTY 4 cut(s) 485, 734, 838, 1282
XceI RCATGY 3 cut(s) 230, 239, 824
XhoI CTCGAG 1 cut(s) 1231
XmaI CCCGGG 1 cut(s) 298
XmnI GAANNNNTTC 2 cut(s) 615, 618
XspI CTAG 3 cut(s) 318, 593, 1410
ZraI GACGTC 1 cut(s) 535
Zsp2I ATGCAT 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.