MD03G1274900.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005281
Physical Location & Seq
Forward (+)
35793075 .. 35795774
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1274900.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGACAGGGCTTGTAATGGTGACAAGGGGCGGCGGGTGCGGTGGTGGAAATGTGAAAGGAGCAGCAAAGGGAAGCAGCAAGAGCTCGGAGCTTGAAGAAAAAAATCAAAGCCAGCTCTCTCTAGTAGCAGTTCTCTTAGCTGCTGTAAGGAAATCCATGGTGGCCTGCCATGTCGAGCGAGGCAGTGAAGAACTCATATCCGCCGTCCAACAAATGGAGATCGGGTGGCCCTCGAACGTCCGCCATATTACCCATGTCACATTCGACCGATTCAACGGGTTTCTGGGTCTTCCTGTCGAGTTCGAGGTTGAGATTCCGGGTCGAGTTCCGAGTGCTAGTGCAAGCGTTTTCGGCGTCTCAGTGGACTCGATGCAGTTGTCTTTTGATTTGAAAGGAAATAGTGTTCCTACCATTCTCTTGTTGATGCAGGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTTCGTATAAACCCCGAGAACAGCCAAGAGGAGATTGTGAGAAGCCAACTGAACCGGGGCATGGTGCCGGATGACGTTGATGTTCATTGCTTGTCAGGTCTGATTAAGGCCTGGTTTCGAGAGCTTCCTTCCGGGGTGCTAGATGGACTGTCCCCCGAACAAGTTCTGCAATGCGAAACAGAAGAGGATTATGTCGAGCTTGTGAAGCAGCTGAAGCCAACTGAGGCTGCATTGCTCAATTGGGCTGTTGATCTGATGGCTGATGTTGTCGAAGAAGAAGAGTTCAACAAAATGAACCCGCGAAACATCGCCATGGTTTTCTCTCCAAACATGACTCAGATGTCGGATCCTCTAACGGCCTTGATGCATGCTGTTCAAGTTATGAATCTGCTTAAGACCCTGATCATGAAAACGCTAAGAGAGCGTGAAGAGACCGATACTGGATCCTATTCACCAATGTCATCTCGTTCGTCTGATCATCTAACGGATGGAGACTTTGATAGCCAGCAAGAGATGGAAAGTAGCTCTGAGTTGAGTGGACCAACATCAGATTGTGATGAAAACACCCACAACAGCCGCAGAAGCGAAAATGGAGATGAAACTCGGTCACTAAGTGAGATAGAAGAAGGCTTTTTGAGACAATTGGATGAGATTAAAACTGTCACAAGTTGTTTGGTAATGTTGGATCAACCAACAGGTGAGCTGGGTGGGGAGTGTCAGAGTCCTCAAAGTTGCTCGGGCTTCAACGCAGAATCCGGCACATCATTTTCTGATAGCAAGGATGTGAATTCGGGTTTTAGCTCAAGCGAAGGGGACGAGGTCTCAAGAAAATGTATGATATCTGAGGAAGAACAGGACATAGACGCGGAGAGCTCTTCAACAGCCTGTGTAAACACGGATGAAATGGAGGAGGAGGCCAAATTCATAGAGCCTGTTTCGGCTTCACCTACTCCGTTTGTGGCATCCAGTGGATCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

484

Amino Acids

52.57

Weight (kDa)

4.41

Isoelectric Point (pI)

67.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 136 - 270 1.7e-19 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 811
AccB1I GGYRCC 1 cut(s) 535
AccB7I CCANNNNNTGG 1 cut(s) 214
AccII CGCG 2 cut(s) 772, 1337
AciI CCGC 8 cut(s) 30, 33, 39, 201, 241, 770, 1048, 1337
AclWI GGATC 6 cut(s) 812, 825, 909, 922, 1164, 1450
AcsI RAATTY 2 cut(s) 1258, 1391
AcuI CTGAAG 1 cut(s) 704
AcyI GRCGYC 1 cut(s) 354
AdeI CACNNNGTG 1 cut(s) 1085
AfiI CCNNNNNNNGG 2 cut(s) 214, 532
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 8 cut(s) 95, 274, 391, 457, 757, 848, 1216, 1350
AjnI CCWGG 1 cut(s) 581
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
Alw21I GWGCWC 2 cut(s) 86, 1346
Alw26I GTCTC 5 cut(s) 361, 896, 957, 1102, 1297
AlwI GGATC 6 cut(s) 812, 825, 909, 922, 1164, 1450
Ama87I CYCGRG 2 cut(s) 485, 1207
AoxI GGCC 5 cut(s) 162, 227, 579, 828, 1386
ApeKI GCWGC 5 cut(s) 62, 75, 140, 679, 698
ApoI RAATTY 2 cut(s) 1258, 1391
Asp700I GAANNNNTTC 1 cut(s) 633
AspS9I GGNCC 2 cut(s) 228, 1010
AsuC2I CCSGG 3 cut(s) 318, 527, 604
AsuHPI GGTGA 4 cut(s) 31, 915, 1181, 1407
AvaI CYCGRG 2 cut(s) 485, 1207
AvaII GGWCC 1 cut(s) 1010
BamHI GGATCC 2 cut(s) 817, 914
BanI GGYRCC 1 cut(s) 535
BanII GRGCYC 2 cut(s) 86, 1346
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 86, 1346
BbvI GCAGC 5 cut(s) 74, 87, 127, 685, 691
BccI CCATC 4 cut(s) 608, 721, 953, 979
BceAI ACGGC 2 cut(s) 188, 843
BciT130I CCWGG 1 cut(s) 583
BclI TGATCA 2 cut(s) 873, 946
BcnI CCSGG 3 cut(s) 318, 527, 604
BcoDI GTCTC 5 cut(s) 361, 896, 957, 1102, 1297
BfaI CTAG 3 cut(s) 122, 336, 611
BfrI CTTAAG 1 cut(s) 863
BisI GCNGC 7 cut(s) 31, 63, 76, 141, 680, 699, 1048
BlsI GCNGC 7 cut(s) 32, 64, 77, 142, 681, 700, 1049
Bme1390I CCNGG 4 cut(s) 318, 527, 583, 604
Bme18I GGWCC 1 cut(s) 1010
BmeT110I CYCGRG 2 cut(s) 485, 1207
BmgT120I GGNCC 2 cut(s) 228, 1010
BmiI GGNNCC 3 cut(s) 537, 819, 916
BmrFI CCNGG 4 cut(s) 318, 527, 583, 604
BmsI GCATC 4 cut(s) 360, 414, 825, 1442
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 1258, 1279
BpuMI CCSGG 3 cut(s) 318, 527, 604
BsaHI GRCGYC 1 cut(s) 354
BsaI GGTCTC 2 cut(s) 896, 1297
BsaJI CCNNGG 4 cut(s) 156, 526, 603, 783
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 532
Bse1I ACTGG 2 cut(s) 916, 1437
Bse3DI GCAATG 3 cut(s) 556, 647, 701
BseBI CCWGG 1 cut(s) 583
BseDI CCNNGG 4 cut(s) 156, 526, 603, 783
BseGI GGATG 6 cut(s) 547, 964, 1123, 1258, 1375, 1433
BseLI CCNNNNNNNGG 2 cut(s) 214, 532
BseMI GCAATG 3 cut(s) 556, 647, 701
BseMII CTCAG 5 cut(s) 372, 684, 821, 990, 1305
BseNI ACTGG 2 cut(s) 916, 1437
BseRI GAGGAG 3 cut(s) 515, 1394, 1397
BseXI GCAGC 5 cut(s) 74, 87, 127, 685, 691
BseYI CCCAGC 1 cut(s) 1174
Bsh1236I CGCG 2 cut(s) 772, 1337
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 5 cut(s) 164, 229, 581, 830, 1388
BshNI GGYRCC 1 cut(s) 535
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 2 cut(s) 86, 1346
BsiHKCI CYCGRG 2 cut(s) 485, 1207
BsiSI CCGG 5 cut(s) 317, 526, 539, 603, 1227
BslFI GGGAC 2 cut(s) 607, 1298
BslI CCNNNNNNNGG 2 cut(s) 214, 532
BsmAI GTCTC 5 cut(s) 361, 896, 957, 1102, 1297
BsmBI CGTCTC 1 cut(s) 361
BsmFI GGGAC 2 cut(s) 607, 1298
BsnI GGCC 5 cut(s) 164, 229, 581, 830, 1388
Bso31I GGTCTC 2 cut(s) 896, 1297
BsoBI CYCGRG 2 cut(s) 485, 1207
Bsp1286I GDGCHC 2 cut(s) 86, 1346
Bsp143I GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
Bsp19I CCATGG 2 cut(s) 156, 783
BspACI CCGC 8 cut(s) 30, 33, 39, 201, 241, 770, 1048, 1337
BspANI GGCC 5 cut(s) 164, 229, 581, 830, 1388
BspCNI CTCAG 5 cut(s) 371, 685, 820, 991, 1306
BspFNI CGCG 2 cut(s) 772, 1337
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 3 cut(s) 537, 819, 916
BspPI GGATC 6 cut(s) 812, 825, 909, 922, 1164, 1450
BspQI GCTCTTC 1 cut(s) 1351
BspT107I GGYRCC 1 cut(s) 535
BspTI CTTAAG 1 cut(s) 863
BspTNI GGTCTC 2 cut(s) 896, 1297
BsrDI GCAATG 3 cut(s) 556, 647, 701
BsrI ACTGG 2 cut(s) 916, 1437
BssECI CCNNGG 4 cut(s) 156, 526, 603, 783
BssMI GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 156, 783
Bst2UI CCWGG 1 cut(s) 583
Bst4CI ACNGT 2 cut(s) 621, 1132
Bst6I CTCTTC 4 cut(s) 648, 744, 894, 1351
BstACI GRCGYC 1 cut(s) 354
BstAFI CTTAAG 1 cut(s) 863
BstC8I GCNNGC 5 cut(s) 113, 166, 343, 840, 977
BstDEI CTNAG 8 cut(s) 136, 358, 693, 807, 887, 999, 1082, 1314
BstDSI CCRYGG 2 cut(s) 156, 783
BstF5I GGATG 6 cut(s) 547, 964, 1123, 1258, 1375, 1433
BstFNI CGCG 2 cut(s) 772, 1337
BstKTI GATC 8 cut(s) 222, 724, 820, 876, 917, 949, 1159, 1445
BstMAI GTCTC 5 cut(s) 361, 896, 957, 1102, 1297
BstMBI GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 7 cut(s) 36, 81, 351, 676, 685, 892, 1053
BstNI CCWGG 1 cut(s) 583
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 4 cut(s) 316, 525, 581, 602
BstUI CGCG 2 cut(s) 772, 1337
BstV1I GCAGC 5 cut(s) 74, 87, 127, 685, 691
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 2 cut(s) 817, 914
BstYI RGATCY 2 cut(s) 817, 914
BsuRI GGCC 5 cut(s) 164, 229, 581, 830, 1388
BtgI CCRYGG 2 cut(s) 156, 783
BtgZI GCGATG 1 cut(s) 763
BtsCI GGATG 6 cut(s) 547, 964, 1123, 1258, 1375, 1433
BtsI GCAGTG 1 cut(s) 190
BtsIMutI CAGTG 3 cut(s) 190, 366, 1444
Cac8I GCNNGC 5 cut(s) 113, 166, 343, 840, 977
CciI TCATGA 1 cut(s) 876
Cfr13I GGNCC 2 cut(s) 228, 1010
CseI GACGC 2 cut(s) 343, 1343
CviAII CATG 8 cut(s) 157, 170, 254, 532, 784, 802, 839, 877
DdeI CTNAG 8 cut(s) 136, 358, 693, 807, 887, 999, 1082, 1314
DpnI GATC 8 cut(s) 221, 723, 819, 875, 916, 948, 1158, 1444
DpnII GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
DraIII CACNNNGTG 1 cut(s) 1085
DrdI GACNNNNNNGTC 1 cut(s) 811
DseDI GACNNNNNNGTC 1 cut(s) 811
Eam1104I CTCTTC 4 cut(s) 648, 744, 894, 1351
EarI CTCTTC 4 cut(s) 648, 744, 894, 1351
EciI GGCGGA 2 cut(s) 190, 230
Ecl136II GAGCTC 2 cut(s) 84, 1344
Eco130I CCWWGG 2 cut(s) 156, 783
Eco147I AGGCCT 1 cut(s) 581
Eco24I GRGCYC 2 cut(s) 86, 1346
Eco31I GGTCTC 2 cut(s) 896, 1297
Eco32I GATATC 1 cut(s) 1311
Eco47I GGWCC 1 cut(s) 1010
Eco53kI GAGCTC 2 cut(s) 84, 1344
Eco57I CTGAAG 1 cut(s) 704
Eco88I CYCGRG 2 cut(s) 485, 1207
EcoICRI GAGCTC 2 cut(s) 84, 1344
EcoRI GAATTC 1 cut(s) 1258
EcoRII CCWGG 1 cut(s) 581
EcoRV GATATC 1 cut(s) 1311
EcoT14I CCWWGG 2 cut(s) 156, 783
EcoT22I ATGCAT 1 cut(s) 840
EcoT38I GRGCYC 2 cut(s) 86, 1346
ErhI CCWWGG 2 cut(s) 156, 783
Esp3I CGTCTC 1 cut(s) 361
FaeI CATG 8 cut(s) 160, 173, 257, 535, 787, 805, 842, 880
FaqI GGGAC 2 cut(s) 607, 1298
FatI CATG 8 cut(s) 156, 169, 253, 531, 783, 801, 838, 876
FauI CCCGC 2 cut(s) 26, 777
FbaI TGATCA 2 cut(s) 873, 946
Fnu4HI GCNGC 7 cut(s) 31, 63, 76, 141, 680, 699, 1048
FokI GGATG 6 cut(s) 554, 971, 1130, 1265, 1382, 1420
FriOI GRGCYC 2 cut(s) 86, 1346
Fsp4HI GCNGC 7 cut(s) 31, 63, 76, 141, 680, 699, 1048
FspBI CTAG 3 cut(s) 122, 336, 611
GluI GCNGC 7 cut(s) 31, 63, 76, 141, 680, 699, 1048
GsaI CCCAGC 1 cut(s) 1178
HaeIII GGCC 5 cut(s) 164, 229, 581, 830, 1388
HapII CCGG 5 cut(s) 317, 526, 539, 603, 1227
HgaI GACGC 2 cut(s) 343, 1343
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 8 cut(s) 160, 173, 257, 535, 787, 805, 842, 880
HinfI GANTC 7 cut(s) 270, 313, 365, 805, 856, 1192, 1223
HpaII CCGG 5 cut(s) 317, 526, 539, 603, 1227
HphI GGTGA 4 cut(s) 31, 915, 1181, 1407
Hpy166II GTNNAC 3 cut(s) 364, 1010, 1363
Hpy188III TCNNGA 3 cut(s) 590, 877, 1296
Hpy8I GTNNAC 3 cut(s) 364, 1010, 1363
HpyAV CCTTC 5 cut(s) 451, 458, 609, 1091, 1274
HpyCH4III ACNGT 2 cut(s) 621, 1132
HpyCH4IV ACGT 2 cut(s) 237, 546
HpyCH4V TGCA 6 cut(s) 341, 373, 427, 640, 701, 838
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 81, 351, 676, 685, 892, 1053
HpyF3I CTNAG 8 cut(s) 136, 358, 693, 807, 887, 999, 1082, 1314
HpySE526I ACGT 2 cut(s) 237, 546
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 8 cut(s) 160, 173, 257, 535, 787, 805, 842, 880
Ksp22I TGATCA 2 cut(s) 873, 946
Kzo9I GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
LguI GCTCTTC 1 cut(s) 1351
LmnI GCTCC 2 cut(s) 59, 88
Lsp1109I GCAGC 5 cut(s) 74, 87, 127, 685, 691
LweI GCATC 4 cut(s) 360, 414, 825, 1442
MaeI CTAG 3 cut(s) 122, 336, 611
MaeII ACGT 2 cut(s) 237, 546
MaeIII GTNAC 4 cut(s) 19, 256, 1077, 1132
MalI GATC 8 cut(s) 221, 723, 819, 875, 916, 948, 1158, 1444
MboI GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
MfeI CAATTG 2 cut(s) 709, 1112
MflI RGATCY 2 cut(s) 817, 914
MhlI GDGCHC 2 cut(s) 86, 1346
MluCI AATT 4 cut(s) 709, 1112, 1258, 1391
MlyI GAGTC 3 cut(s) 359, 799, 1201
MmeI TCCRAC 3 cut(s) 232, 795, 1134
Mph1103I ATGCAT 1 cut(s) 840
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 3 cut(s) 576, 864, 1125
MslI CAYNNNNRTG 1 cut(s) 782
MspA1I CMGCKG 1 cut(s) 682
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 5 cut(s) 317, 526, 539, 603, 1227
MspR9I CCNGG 4 cut(s) 318, 527, 583, 604
MunI CAATTG 2 cut(s) 709, 1112
MvaI CCWGG 1 cut(s) 583
MvnI CGCG 2 cut(s) 772, 1337
MwoI GCNNNNNNNGC 7 cut(s) 36, 81, 351, 676, 685, 892, 1053
NciI CCSGG 3 cut(s) 318, 527, 604
NcoI CCATGG 2 cut(s) 156, 783
NdeII GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
NlaIII CATG 8 cut(s) 160, 173, 257, 535, 787, 805, 842, 880
NlaIV GGNNCC 3 cut(s) 537, 819, 916
NmuCI GTSAC 4 cut(s) 19, 256, 1077, 1132
NsiI ATGCAT 1 cut(s) 840
NspI RCATGY 1 cut(s) 842
PaeI GCATGC 1 cut(s) 842
PagI TCATGA 1 cut(s) 876
PceI AGGCCT 1 cut(s) 581
PciSI GCTCTTC 1 cut(s) 1351
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 4 cut(s) 270, 313, 856, 1223
PflFI GACNNNGTC 1 cut(s) 1289
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 7 cut(s) 32, 64, 77, 142, 681, 700, 1049
PleI GAGTC 3 cut(s) 359, 799, 1200
PpsI GAGTC 3 cut(s) 359, 799, 1200
Psp124BI GAGCTC 2 cut(s) 86, 1346
Psp6I CCWGG 1 cut(s) 581
PspFI CCCAGC 1 cut(s) 1174
PspGI CCWGG 1 cut(s) 581
PspN4I GGNNCC 3 cut(s) 537, 819, 916
PspPI GGNCC 2 cut(s) 228, 1010
PsuI RGATCY 2 cut(s) 817, 914
PsyI GACNNNGTC 1 cut(s) 1289
PvuII CAGCTG 1 cut(s) 682
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 2 cut(s) 86, 1346
SapI GCTCTTC 1 cut(s) 1351
SaqAI TTAA 3 cut(s) 576, 864, 1125
SatI GCNGC 7 cut(s) 31, 63, 76, 141, 680, 699, 1048
Sau3AI GATC 8 cut(s) 219, 721, 817, 873, 914, 946, 1156, 1442
Sau96I GGNCC 2 cut(s) 228, 1010
SchI GAGTC 3 cut(s) 359, 799, 1201
ScrFI CCNGG 4 cut(s) 318, 527, 583, 604
SduI GDGCHC 2 cut(s) 86, 1346
SfaNI GCATC 4 cut(s) 360, 414, 825, 1442
SinI GGWCC 1 cut(s) 1010
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 3 cut(s) 863, 1273, 1294
SmoI CTYRAG 3 cut(s) 863, 1273, 1294
SphI GCATGC 1 cut(s) 842
Sse9I AATT 4 cut(s) 709, 1112, 1258, 1391
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 8 cut(s) 30, 33, 39, 201, 241, 770, 1048, 1337
SspI AATATT 1 cut(s) 471
SspMI CTAG 3 cut(s) 122, 336, 611
SstI GAGCTC 2 cut(s) 86, 1346
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 4 cut(s) 316, 525, 581, 602
StyI CCWWGG 2 cut(s) 156, 783
TaaI ACNGT 2 cut(s) 621, 1132
TaiI ACGT 2 cut(s) 240, 549
TaqII GACCGA 3 cut(s) 282, 920, 1065
TasI AATT 4 cut(s) 709, 1112, 1258, 1391
TauI GCSGC 2 cut(s) 33, 1050
TfiI GAWTC 4 cut(s) 270, 313, 856, 1223
Tru1I TTAA 3 cut(s) 576, 864, 1125
Tru9I TTAA 3 cut(s) 576, 864, 1125
TscAI CASTG 3 cut(s) 190, 366, 1444
TseFI GTSAC 4 cut(s) 19, 256, 1077, 1132
TseI GCWGC 5 cut(s) 62, 75, 140, 679, 698
Tsp45I GTSAC 4 cut(s) 19, 256, 1077, 1132
TspDTI ATGAA 8 cut(s) 545, 779, 869, 893, 1044, 1083, 1384, 1386
TspGWI ACGGA 3 cut(s) 971, 1382, 1413
TspRI CASTG 3 cut(s) 190, 366, 1444
Tth111I GACNNNGTC 1 cut(s) 1289
Van91I CCANNNNNTGG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 863
VpaK11BI GGWCC 1 cut(s) 1010
XapI RAATTY 2 cut(s) 1258, 1391
XceI RCATGY 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 3 cut(s) 122, 336, 611
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.