MD11G1296900.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005281
Physical Location & Seq
Forward (+)
41447553 .. 41450390
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1296900.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGACAGGGCTTGTGATGCTGACGAGGGGCGGCGGCTGCGGAGGCGGAGGTGGCGGAAATGGGAAAGGAGCAAAAGGAGCTAACAAGAGCTCAGAAGAAGAGCAGAACCAGATCTCTCTGGCTGCTCTTCTGTTAGCTGCTGTGAGGAAATCCATGGTAGCCTGCCGTGTCGAAAGAGGCGAAGAAGTCATCTCCGCCGTCCACAACATGGAGATCGGATGGCCCACGAACGTCCAGCACGTTACCCATGTCACATTCGATCGGTTCAATGGGTTTCTGGGCCTTCCGGTGGAGTTCGAGGTTGAGGTTCCAGGCAGAGTTCCCAGTGCTAGTGCAAGCGTGTTCGGGGTCTCAGCGGAGTCAATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAAGAAGGATTGAAGGCAGAAGGAATATTTCGTATAAACCCCGAGAACAGCCATGAAGAGCTTGTGAGAAGCCAATTGAACAGGGGAATTGTGCCTGATGACATTGATGTCCATTGTTTGGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCGGGAATACTAGATGGACTGTCTCCTGAAGAAGTTCTTCAATGCAACACGGAAGAGGAATCCGTTGAGCTTGTGAAGCAATTAAAGCCAACCGAGACCGCATTGCTCAACTGGGCAGTCAATCTGATGGCTGATGTTGTTGAGGAAGAAGAACTCAACAAAATGAATGCGCGAAACATCGCTATGGTTTTCGCTCCAAACATGACTCAGATGTCTGATCCTCTGACGGCTTTAATGCACGCTGTTCAAGTAATGAATTTGCTAAAGACACTGATTATGAAGACGCTAAGAGAGCGTGAAGAAAACGATGAAACTGGAGGATATTCACCAATGTCATCTTGTTCATCTGATCATCAAACCGACGAAGACTCTGATGATAGTCAGCAGGAAATGGATACTAGATCTGAATTGGAGGGTCCAACATCAGACTATGATGAAAACGCGCACTCCATCCACAGCAGCGAAGATGAAGATGAAGTCCGATCAGTAAGTGAGATAGAAGAGCGTTTTTTAAGGCACTTAGGGGAGAACAAAACTGCCGGAAACAGCTTGGTGGTGGCAGATCAACCAACAGGCGAGTTGTGCGGGGAATATCTGAGCCCTCAAAGTTGCTTGAGCTTCAACATGGACTCTGGCACATCGTTTTGTGACAGCAAAAATGGGAACTCCGCCCTCAGTACTACGAGTGATGGGGAAGCCTCATCAGGGAAATGTATGGCAGCTGAGGAAACTAATGTTCAAATGGAGAGCCCTTCGTCAGCTTCGAGTTCAAACAGGATTAAAATAGAGACAGTGGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

466

Amino Acids

50.48

Weight (kDa)

4.51

Isoelectric Point (pI)

56.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 134 - 268 1.7e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 545, 805
AccB7I CCANNNNNTGG 2 cut(s) 208, 556
AccII CGCG 2 cut(s) 766, 1037
AclWI GGATC 1 cut(s) 806
AcsI RAATTY 1 cut(s) 850
AcuI CTGAAG 1 cut(s) 642
AfaI GTAC 1 cut(s) 1273
AfiI CCNNNNNNNGG 4 cut(s) 208, 289, 556, 1299
AgsI TTSAA 9 cut(s) 268, 384, 451, 517, 635, 842, 1216, 1334, 1365
AjnI CCWGG 2 cut(s) 310, 575
AluBI AGCT 9 cut(s) 80, 90, 137, 499, 664, 1143, 1212, 1316, 1355
AluI AGCT 9 cut(s) 80, 90, 137, 499, 664, 1143, 1212, 1316, 1355
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 4 cut(s) 355, 621, 683, 1376
AlwI GGATC 1 cut(s) 806
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 3 cut(s) 221, 280, 562
ApeKI GCWGC 5 cut(s) 36, 122, 137, 1053, 1313
ApoI RAATTY 1 cut(s) 850
Asp700I GAANNNNTTC 2 cut(s) 627, 630
AspLEI GCGC 2 cut(s) 766, 1039
AspS9I GGNCC 3 cut(s) 222, 280, 1010
AsuHPI GGTGA 1 cut(s) 912
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 1010
BanII GRGCYC 3 cut(s) 92, 1196, 1346
BbsI GAAGAC 2 cut(s) 881, 966
Bbv12I GWGCWC 1 cut(s) 92
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 5 cut(s) 23, 109, 124, 1065, 1325
BccI CCATC 5 cut(s) 213, 602, 715, 1052, 1277
BceAI ACGGC 3 cut(s) 150, 182, 837
BciT130I CCWGG 2 cut(s) 312, 577
BciVI GTATCC 1 cut(s) 982
BclI TGATCA 1 cut(s) 943
BcoDI GTCTC 4 cut(s) 355, 621, 683, 1376
BfaI CTAG 3 cut(s) 330, 605, 993
BfuI GTATCC 1 cut(s) 982
BglII AGATCT 2 cut(s) 111, 995
BisI GCNGC 7 cut(s) 31, 34, 37, 123, 138, 1054, 1314
BlsI GCNGC 7 cut(s) 32, 35, 38, 124, 139, 1055, 1315
BmcAI AGTACT 1 cut(s) 1273
Bme1390I CCNGG 2 cut(s) 312, 577
Bme18I GGWCC 1 cut(s) 1010
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 3 cut(s) 222, 280, 1010
BmiI GGNNCC 2 cut(s) 309, 1011
BmrFI CCNGG 2 cut(s) 312, 577
BmrI ACTGGG 2 cut(s) 318, 715
BmsI GCATC 2 cut(s) 6, 408
BmuI ACTGGG 2 cut(s) 318, 715
BpiI GAAGAC 2 cut(s) 881, 966
BpmI CTGGAG 1 cut(s) 930
Bpu10I CCTNAGC 1 cut(s) 1317
BpuEI CTTGAG 1 cut(s) 1228
BsaI GGTCTC 2 cut(s) 355, 683
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 1025, 1055
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 289, 556, 1299
Bse1I ACTGG 3 cut(s) 324, 710, 913
Bse3DI GCAATG 1 cut(s) 695
BseBI CCWGG 2 cut(s) 312, 577
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 2 cut(s) 224, 1044
BseLI CCNNNNNNNGG 4 cut(s) 208, 289, 556, 1299
BseMI GCAATG 1 cut(s) 695
BseMII CTCAG 6 cut(s) 105, 366, 815, 1181, 1282, 1308
BseNI ACTGG 3 cut(s) 324, 710, 913
BseXI GCAGC 5 cut(s) 23, 109, 124, 1065, 1325
Bsh1236I CGCG 2 cut(s) 766, 1037
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 3 cut(s) 223, 282, 564
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 287, 1134
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 4 cut(s) 208, 289, 556, 1299
BsmAI GTCTC 4 cut(s) 355, 621, 683, 1376
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 3 cut(s) 223, 282, 564
Bso31I GGTCTC 2 cut(s) 355, 683
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 92, 1196, 1346
Bsp143I GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
Bsp19I CCATGG 1 cut(s) 153
BspANI GGCC 3 cut(s) 223, 282, 564
BspCNI CTCAG 6 cut(s) 104, 365, 814, 1182, 1281, 1309
BspFNI CGCG 2 cut(s) 766, 1037
BspLI GGNNCC 2 cut(s) 309, 1011
BspPI GGATC 1 cut(s) 806
BspQI GCTCTTC 4 cut(s) 93, 132, 489, 1089
BspTNI GGTCTC 2 cut(s) 355, 683
BsrDI GCAATG 1 cut(s) 695
BsrI ACTGG 3 cut(s) 324, 710, 913
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 312, 577
Bst4CI ACNGT 2 cut(s) 615, 1387
Bst6I CTCTTC 5 cut(s) 93, 132, 489, 642, 1089
BstC8I GCNNGC 4 cut(s) 163, 337, 562, 834
BstDEI CTNAG 8 cut(s) 91, 352, 801, 881, 1114, 1190, 1268, 1317
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 224, 1044
BstFNI CGCG 2 cut(s) 766, 1037
BstHHI GCGC 2 cut(s) 766, 1039
BstKTI GATC 8 cut(s) 114, 216, 262, 814, 946, 998, 1079, 1159
BstMAI GTCTC 4 cut(s) 355, 621, 683, 1376
BstMBI GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 9 cut(s) 16, 36, 42, 51, 77, 670, 679, 886, 1176
BstNI CCWGG 2 cut(s) 312, 577
BstSCI CCNGG 2 cut(s) 310, 575
BstUI CGCG 2 cut(s) 766, 1037
BstV1I GCAGC 5 cut(s) 23, 109, 124, 1065, 1325
BstV2I GAAGAC 2 cut(s) 881, 966
BstX2I RGATCY 2 cut(s) 111, 995
BstYI RGATCY 2 cut(s) 111, 995
BsuI GTATCC 1 cut(s) 982
BsuRI GGCC 3 cut(s) 223, 282, 564
BtgI CCRYGG 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 757
BtsCI GGATG 2 cut(s) 224, 1044
BtsIMutI CAGTG 3 cut(s) 331, 863, 1392
Cac8I GCNNGC 4 cut(s) 163, 337, 562, 834
CfoI GCGC 2 cut(s) 766, 1039
Cfr13I GGNCC 3 cut(s) 222, 280, 1010
CseI GACGC 1 cut(s) 886
Csp6I GTAC 1 cut(s) 1272
CviAII CATG 6 cut(s) 154, 208, 248, 491, 796, 1219
CviQI GTAC 1 cut(s) 1272
DdeI CTNAG 8 cut(s) 91, 352, 801, 881, 1114, 1190, 1268, 1317
DpnI GATC 8 cut(s) 113, 215, 261, 813, 945, 997, 1078, 1158
DpnII GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
DrdI GACNNNNNNGTC 2 cut(s) 545, 805
DseDI GACNNNNNNGTC 2 cut(s) 545, 805
Eam1104I CTCTTC 5 cut(s) 93, 132, 489, 642, 1089
EarI CTCTTC 5 cut(s) 93, 132, 489, 642, 1089
EciI GGCGGA 4 cut(s) 60, 69, 184, 1252
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 564
Eco24I GRGCYC 3 cut(s) 92, 1196, 1346
Eco31I GGTCTC 2 cut(s) 355, 683
Eco47I GGWCC 1 cut(s) 1010
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 642
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 90
EcoRII CCWGG 2 cut(s) 310, 575
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 3 cut(s) 92, 1196, 1346
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 6 cut(s) 157, 211, 251, 494, 799, 1222
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FaqI GGGAC 1 cut(s) 383
FatI CATG 6 cut(s) 153, 207, 247, 490, 795, 1218
FauI CCCGC 1 cut(s) 1172
FbaI TGATCA 1 cut(s) 943
Fnu4HI GCNGC 7 cut(s) 31, 34, 37, 123, 138, 1054, 1314
FokI GGATG 2 cut(s) 231, 1031
FriOI GRGCYC 3 cut(s) 92, 1196, 1346
Fsp4HI GCNGC 7 cut(s) 31, 34, 37, 123, 138, 1054, 1314
FspBI CTAG 3 cut(s) 330, 605, 993
GlaI GCGC 2 cut(s) 765, 1038
GluI GCNGC 7 cut(s) 31, 34, 37, 123, 138, 1054, 1314
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 3 cut(s) 223, 282, 564
HapII CCGG 2 cut(s) 287, 1134
HgaI GACGC 1 cut(s) 886
HhaI GCGC 2 cut(s) 766, 1039
Hin1II CATG 6 cut(s) 157, 211, 251, 494, 799, 1222
Hin6I GCGC 2 cut(s) 764, 1037
HinP1I GCGC 2 cut(s) 764, 1037
HinfI GANTC 6 cut(s) 359, 380, 653, 799, 962, 1223
HpaII CCGG 2 cut(s) 287, 1134
HphI GGTGA 1 cut(s) 912
Hpy166II GTNNAC 2 cut(s) 202, 1390
Hpy188III TCNNGA 3 cut(s) 584, 597, 620
Hpy8I GTNNAC 2 cut(s) 202, 1390
Hpy99I CGWCG 1 cut(s) 959
HpyAV CCTTC 6 cut(s) 293, 437, 445, 452, 603, 1356
HpyCH4III ACNGT 2 cut(s) 615, 1387
HpyCH4IV ACGT 2 cut(s) 231, 240
HpyCH4V TGCA 5 cut(s) 335, 367, 421, 639, 832
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 36, 42, 51, 77, 670, 679, 886, 1176
HpyF3I CTNAG 8 cut(s) 91, 352, 801, 881, 1114, 1190, 1268, 1317
HpySE526I ACGT 2 cut(s) 231, 240
Hsp92II CATG 6 cut(s) 157, 211, 251, 494, 799, 1222
HspAI GCGC 2 cut(s) 764, 1037
Ksp22I TGATCA 1 cut(s) 943
Kzo9I GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
LguI GCTCTTC 4 cut(s) 93, 132, 489, 1089
LmnI GCTCC 3 cut(s) 68, 77, 793
Lsp1109I GCAGC 5 cut(s) 23, 109, 124, 1065, 1325
LweI GCATC 2 cut(s) 6, 408
MaeI CTAG 3 cut(s) 330, 605, 993
MaeII ACGT 2 cut(s) 231, 240
MaeIII GTNAC 3 cut(s) 241, 250, 1241
MalI GATC 8 cut(s) 113, 215, 261, 813, 945, 997, 1078, 1158
MboI GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 2 cut(s) 111, 995
MhlI GDGCHC 3 cut(s) 92, 1196, 1346
MluCI AATT 5 cut(s) 512, 525, 674, 850, 1001
MlyI GAGTC 4 cut(s) 368, 793, 956, 1217
MmeI TCCRAC 1 cut(s) 1037
MroXI GAANNNNTTC 2 cut(s) 627, 630
MseI TTAA 4 cut(s) 677, 827, 1106, 1374
MslI CAYNNNNRTG 1 cut(s) 776
MspA1I CMGCKG 2 cut(s) 356, 1316
MspI CCGG 2 cut(s) 287, 1134
MspR9I CCNGG 2 cut(s) 312, 577
MunI CAATTG 1 cut(s) 512
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 2 cut(s) 312, 577
MvnI CGCG 2 cut(s) 766, 1037
MwoI GCNNNNNNNGC 9 cut(s) 16, 36, 42, 51, 77, 670, 679, 886, 1176
NcoI CCATGG 1 cut(s) 153
NdeII GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
NlaIII CATG 6 cut(s) 157, 211, 251, 494, 799, 1222
NlaIV GGNNCC 2 cut(s) 309, 1011
NmuCI GTSAC 2 cut(s) 250, 1241
PceI AGGCCT 1 cut(s) 564
PciSI GCTCTTC 4 cut(s) 93, 132, 489, 1089
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 237, 1355
PctI GAATGC 1 cut(s) 766
PdmI GAANNNNTTC 2 cut(s) 627, 630
PfeI GAWTC 2 cut(s) 380, 653
PflMI CCANNNNNTGG 2 cut(s) 208, 556
PkrI GCNGC 7 cut(s) 32, 35, 38, 124, 139, 1055, 1315
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 4 cut(s) 367, 793, 956, 1217
PpsI GAGTC 4 cut(s) 367, 793, 956, 1217
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 310, 575
PspGI CCWGG 2 cut(s) 310, 575
PspN4I GGNNCC 2 cut(s) 309, 1011
PspPI GGNCC 3 cut(s) 222, 280, 1010
PsuI RGATCY 2 cut(s) 111, 995
PvuI CGATCG 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 1 cut(s) 1273
RsaNI GTAC 1 cut(s) 1272
RseI CAYNNNNRTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 92
SapI GCTCTTC 4 cut(s) 93, 132, 489, 1089
SaqAI TTAA 4 cut(s) 677, 827, 1106, 1374
SatI GCNGC 7 cut(s) 31, 34, 37, 123, 138, 1054, 1314
Sau3AI GATC 8 cut(s) 111, 213, 259, 811, 943, 995, 1076, 1156
Sau96I GGNCC 3 cut(s) 222, 280, 1010
ScaI AGTACT 1 cut(s) 1273
SchI GAGTC 4 cut(s) 368, 793, 956, 1217
ScrFI CCNGG 2 cut(s) 312, 577
SduI GDGCHC 3 cut(s) 92, 1196, 1346
SfaNI GCATC 2 cut(s) 6, 408
SinI GGWCC 1 cut(s) 1010
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 1207
SmoI CTYRAG 1 cut(s) 1207
Sse9I AATT 5 cut(s) 512, 525, 674, 850, 1001
SseBI AGGCCT 1 cut(s) 564
SspI AATATT 1 cut(s) 465
SspMI CTAG 3 cut(s) 330, 605, 993
SstI GAGCTC 1 cut(s) 92
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 310, 575
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 615, 1387
TaiI ACGT 2 cut(s) 234, 243
TaqI TCGA 5 cut(s) 171, 258, 297, 583, 1358
TasI AATT 5 cut(s) 512, 525, 674, 850, 1001
TatI WGTACW 1 cut(s) 1271
TauI GCSGC 2 cut(s) 33, 36
TfiI GAWTC 2 cut(s) 380, 653
Tru1I TTAA 4 cut(s) 677, 827, 1106, 1374
Tru9I TTAA 4 cut(s) 677, 827, 1106, 1374
TscAI CASTG 3 cut(s) 331, 870, 1392
TseFI GTSAC 2 cut(s) 250, 1241
TseI GCWGC 5 cut(s) 36, 122, 137, 1053, 1313
Tsp45I GTSAC 2 cut(s) 250, 1241
TspDTI ATGAA 9 cut(s) 507, 773, 863, 887, 918, 927, 1044, 1077, 1083
TspGWI ACGGA 2 cut(s) 646, 659
TspRI CASTG 3 cut(s) 331, 870, 1392
Van91I CCANNNNNTGG 2 cut(s) 208, 556
VpaK11BI GGWCC 1 cut(s) 1010
XapI RAATTY 1 cut(s) 850
XmnI GAANNNNTTC 2 cut(s) 627, 630
XspI CTAG 3 cut(s) 330, 605, 993
ZrmI AGTACT 1 cut(s) 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.