Rh6CG354100

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005281
Physical Location & Seq
Forward (+)
54300925 .. 54304417
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG354100.1

Sequence Viewer

Length: 1113 bp
ATGATCTACTTTTACAGTGCAAGTGTGTTTGGAGTCTCAGTGGATTCAATGCAGCTGTCCTTCGATTCAAGAGGGAATAGTGTGCCTACAATTCTGCTTCTAATGCAGGAGAGGTTATATGCACAAGGAGGATTGAAGGCTGAAGGAATATTTCGTATAAACCCAGAGAATGGCCAAGAGGAGATTGTGAGAGGCCAATTGAACAGGGGCATTGTGCCGGATGACGTTGATGTTCATTGTCTGTCTGGTCTGATTAAGGCCTGGTTTCGAGAGCTTCCTTCAGGAGTGCTAGATGGACTATCCCCCGAACAAGTTCTTCAGTGTGAGACAGAAGAGGACTGCGTTGAGCTTGTGAAGCAGTTAAAGCCAACTGAGGCTGCATTGCTCAATTGGGCTATTGATCTGATGGCCGATGTTGTTGAGGAAGAAGAATTCAACAAGATGAATGCAAGAAACATCGCTATGGTTTTTGCCCCAAACATGACTCAGATGTCTGATCCATTAACGGCTTTGATGCATGCGGTTCAAGTAATGAATTTGCTTAAGACCCTGATCACGAAAACACTAAATGAGCGTGAAGAGACTGAAAGCGGATCATATTCACAGTCCCCAATGTCATCTTGTTCATCTGAGGGTCAAACAGATGACTTTGATAGTCAGCACGAGGTTGAAAGCAGCTCCACGTTAATCAAGACTTCACCAACATCAGATTATGATGAAAATGGCAGCAGCAGCAACAACAGTGAAGATGAATCTGAAGTCAAGTTACTGAATGACATAGAAGAATGCTTACGACAATTGGATGAGAATAAACTTTCAACAAATGGCTTGGTAATGTTGGGTGAACTAACATGCGAGTTGCAAGGTGAGTCTTTGAGTCCTCAGTGCTGCTCTAATGTGGAGTCCGCCACATCCTTTACTGATAGCAAAAATAGGGATTCTGGCTCGAGTACAAGTGACGAGGAAGAAGAAGACACTGCGAAATCTATCATAGCTGTGGCACAGGACTCTGATATTAAAAACCATACAAGCATAAATGAAATGGAGATGGTGGACAGATCTGCGGAGTCTGTTTTGCCTATGCCATTGACTGCATCTACTGGATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

370

Amino Acids

40.64

Weight (kDa)

4.16

Isoelectric Point (pI)

57.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 29 - 163 3.1e-19 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 170
AciI CCGC 4 cut(s) 521, 591, 906, 1064
AclWI GGATC 2 cut(s) 491, 601
AcoI YGGCCR 2 cut(s) 172, 408
AcsI RAATTY 2 cut(s) 431, 535
AcuI CTGAAG 4 cut(s) 162, 264, 302, 777
AfaI GTAC 1 cut(s) 952
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 1 cut(s) 542
AgsI TTSAA 8 cut(s) 48, 69, 136, 202, 436, 527, 671, 819
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 5 cut(s) 55, 274, 349, 678, 995
AluI AGCT 5 cut(s) 55, 274, 349, 678, 995
Alw26I GTCTC 3 cut(s) 40, 320, 575
AlwI GGATC 2 cut(s) 491, 601
Ama87I CYCGRG 1 cut(s) 946
AoxI GGCC 4 cut(s) 172, 193, 258, 408
ApeKI GCWGC 7 cut(s) 52, 377, 675, 726, 729, 732, 888
ApoI RAATTY 2 cut(s) 431, 535
Asp700I GAANNNNTTC 1 cut(s) 312
AsuHPI GGTGA 3 cut(s) 690, 854, 878
AvaI CYCGRG 1 cut(s) 946
BalI TGGCCA 1 cut(s) 174
BarI GAAGNNNNNNTAC 4 cut(s) 750, 774, 782, 806
BauI CACGAG 1 cut(s) 662
BbsI GAAGAC 1 cut(s) 978
BbvI GCAGC 7 cut(s) 64, 364, 687, 738, 741, 744, 875
BccI CCATC 3 cut(s) 287, 400, 1042
BceAI ACGGC 1 cut(s) 522
BciT130I CCWGG 1 cut(s) 262
BclI TGATCA 1 cut(s) 552
BcoDI GTCTC 3 cut(s) 40, 320, 575
BfaI CTAG 1 cut(s) 290
BfrI CTTAAG 1 cut(s) 542
BglII AGATCT 1 cut(s) 1058
BisI GCNGC 7 cut(s) 53, 378, 676, 727, 730, 733, 889
BlsI GCNGC 7 cut(s) 54, 379, 677, 728, 731, 734, 890
Bme1390I CCNGG 1 cut(s) 262
BmeT110I CYCGRG 1 cut(s) 946
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 2 cut(s) 504, 1103
BpiI GAAGAC 1 cut(s) 978
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 1105
Bse3DI GCAATG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 262
BseGI GGATG 3 cut(s) 226, 808, 911
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 5 cut(s) 51, 363, 500, 621, 896
BseNI ACTGG 1 cut(s) 1105
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 7 cut(s) 64, 364, 687, 738, 741, 744, 875
BshFI GGCC 4 cut(s) 174, 195, 260, 410
BsiHKCI CYCGRG 1 cut(s) 946
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 3 cut(s) 40, 320, 575
BsmFI GGGAC 1 cut(s) 592
BsmI GAATGC 2 cut(s) 451, 791
BsnI GGCC 4 cut(s) 174, 195, 260, 410
BsoBI CYCGRG 1 cut(s) 946
Bsp143I GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
BspACI CCGC 4 cut(s) 521, 591, 906, 1064
BspANI GGCC 4 cut(s) 174, 195, 260, 410
BspCNI CTCAG 5 cut(s) 50, 364, 499, 622, 895
BspPI GGATC 2 cut(s) 491, 601
BspTI CTTAAG 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 1105
BssMI GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
BssSI CACGAG 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 662
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 3 cut(s) 17, 606, 743
Bst6I CTCTTC 2 cut(s) 327, 573
BstAFI CTTAAG 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 5 cut(s) 37, 372, 486, 630, 882
BstF5I GGATG 3 cut(s) 226, 808, 911
BstKTI GATC 6 cut(s) 6, 403, 499, 555, 596, 1061
BstMAI GTCTC 3 cut(s) 40, 320, 575
BstMBI GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
BstMWI GCNNNNNNNGC 4 cut(s) 103, 355, 364, 732
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 2 cut(s) 521, 855
BstSCI CCNGG 1 cut(s) 260
BstV1I GCAGC 7 cut(s) 64, 364, 687, 738, 741, 744, 875
BstV2I GAAGAC 1 cut(s) 978
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
BsuRI GGCC 4 cut(s) 174, 195, 260, 410
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 3 cut(s) 226, 808, 911
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 6 cut(s) 22, 45, 326, 748, 890, 975
Cac8I GCNNGC 1 cut(s) 519
Csp6I GTAC 1 cut(s) 951
CviAII CATG 3 cut(s) 481, 518, 852
CviQI GTAC 1 cut(s) 951
DdeI CTNAG 5 cut(s) 37, 372, 486, 630, 882
DpnI GATC 6 cut(s) 5, 402, 498, 554, 595, 1060
DpnII GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
DrdI GACNNNNNNGTC 1 cut(s) 490
DseDI GACNNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 2 cut(s) 172, 408
Eam1104I CTCTTC 2 cut(s) 327, 573
EarI CTCTTC 2 cut(s) 327, 573
EciI GGCGGA 1 cut(s) 895
Eco147I AGGCCT 1 cut(s) 260
Eco57I CTGAAG 4 cut(s) 162, 264, 302, 777
Eco88I CYCGRG 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 260
EcoT22I ATGCAT 1 cut(s) 519
FaeI CATG 3 cut(s) 484, 521, 855
FaqI GGGAC 1 cut(s) 592
FatI CATG 3 cut(s) 480, 517, 851
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 7 cut(s) 53, 378, 676, 727, 730, 733, 889
FokI GGATG 3 cut(s) 233, 815, 898
Fsp4HI GCNGC 7 cut(s) 53, 378, 676, 727, 730, 733, 889
FspBI CTAG 1 cut(s) 290
GluI GCNGC 7 cut(s) 53, 378, 676, 727, 730, 733, 889
HaeIII GGCC 4 cut(s) 174, 195, 260, 410
HapII CCGG 1 cut(s) 218
Hin1II CATG 3 cut(s) 484, 521, 855
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 690, 854, 878
Hpy166II GTNNAC 2 cut(s) 845, 1054
Hpy188I TCNGA 8 cut(s) 252, 405, 489, 496, 631, 709, 757, 1012
Hpy188III TCNNGA 5 cut(s) 69, 269, 282, 556, 691
Hpy8I GTNNAC 2 cut(s) 845, 1054
HpyAV CCTTC 4 cut(s) 70, 130, 137, 288
HpyCH4III ACNGT 3 cut(s) 17, 606, 743
HpyCH4IV ACGT 2 cut(s) 225, 683
HpyCH4V TGCA 9 cut(s) 20, 52, 106, 122, 380, 449, 517, 862, 1094
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 355, 364, 732
HpyF3I CTNAG 5 cut(s) 37, 372, 486, 630, 882
HpySE526I ACGT 2 cut(s) 225, 683
Hsp92II CATG 3 cut(s) 484, 521, 855
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
LmnI GCTCC 1 cut(s) 683
Lsp1109I GCAGC 7 cut(s) 64, 364, 687, 738, 741, 744, 875
LweI GCATC 2 cut(s) 504, 1103
MaeI CTAG 1 cut(s) 290
MaeII ACGT 2 cut(s) 225, 683
MaeIII GTNAC 2 cut(s) 765, 956
MalI GATC 6 cut(s) 5, 402, 498, 554, 595, 1060
MboI GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
MfeI CAATTG 3 cut(s) 197, 388, 797
MflI RGATCY 1 cut(s) 1058
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 6 cut(s) 90, 197, 388, 431, 535, 797
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 7 cut(s) 42, 478, 878, 886, 911, 1001, 1076
Mox20I TGGCCA 1 cut(s) 174
Mph1103I ATGCAT 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 312
MscI TGGCCA 1 cut(s) 174
MseI TTAA 6 cut(s) 255, 362, 503, 543, 686, 1017
MslI CAYNNNNRTG 2 cut(s) 461, 995
Msp20I TGGCCA 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 55
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 262
MunI CAATTG 3 cut(s) 197, 388, 797
Mva1269I GAATGC 2 cut(s) 451, 791
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 4 cut(s) 103, 355, 364, 732
NdeII GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
NlaIII CATG 3 cut(s) 484, 521, 855
NmuCI GTSAC 1 cut(s) 956
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 2 cut(s) 521, 855
PaeI GCATGC 1 cut(s) 521
PaeR7I CTCGAG 1 cut(s) 946
PceI AGGCCT 1 cut(s) 260
PctI GAATGC 2 cut(s) 451, 791
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 4 cut(s) 44, 65, 752, 938
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 7 cut(s) 54, 379, 677, 728, 731, 734, 890
PleI GAGTC 7 cut(s) 41, 478, 877, 885, 910, 1001, 1075
PpsI GAGTC 7 cut(s) 41, 478, 877, 885, 910, 1001, 1075
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspXI VCTCGAGB 1 cut(s) 946
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 55
RsaI GTAC 1 cut(s) 952
RsaNI GTAC 1 cut(s) 951
RseI CAYNNNNRTG 2 cut(s) 461, 995
SaqAI TTAA 6 cut(s) 255, 362, 503, 543, 686, 1017
SatI GCNGC 7 cut(s) 53, 378, 676, 727, 730, 733, 889
Sau3AI GATC 6 cut(s) 3, 400, 496, 552, 593, 1058
SchI GAGTC 7 cut(s) 42, 478, 878, 886, 911, 1001, 1076
ScrFI CCNGG 1 cut(s) 262
SfaNI GCATC 2 cut(s) 504, 1103
Sfr274I CTCGAG 1 cut(s) 946
SlaI CTCGAG 1 cut(s) 946
SmiMI CAYNNNNRTG 2 cut(s) 461, 995
SmlI CTYRAG 2 cut(s) 542, 946
SmoI CTYRAG 2 cut(s) 542, 946
SphI GCATGC 1 cut(s) 521
Sse9I AATT 6 cut(s) 90, 197, 388, 431, 535, 797
SseBI AGGCCT 1 cut(s) 260
SsiI CCGC 4 cut(s) 521, 591, 906, 1064
SspI AATATT 1 cut(s) 150
SspMI CTAG 1 cut(s) 290
StuI AGGCCT 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 260
TaaI ACNGT 3 cut(s) 17, 606, 743
TaiI ACGT 2 cut(s) 228, 686
TaqI TCGA 3 cut(s) 63, 268, 947
TasI AATT 6 cut(s) 90, 197, 388, 431, 535, 797
TatI WGTACW 1 cut(s) 950
TfiI GAWTC 4 cut(s) 44, 65, 752, 938
Tru1I TTAA 6 cut(s) 255, 362, 503, 543, 686, 1017
Tru9I TTAA 6 cut(s) 255, 362, 503, 543, 686, 1017
TscAI CASTG 6 cut(s) 22, 45, 326, 748, 890, 982
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 7 cut(s) 52, 377, 675, 726, 729, 732, 888
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 7 cut(s) 224, 458, 548, 615, 732, 765, 1053
TspRI CASTG 6 cut(s) 22, 45, 326, 748, 890, 982
Van91I CCANNNNNTGG 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 542
XapI RAATTY 2 cut(s) 431, 535
XceI RCATGY 2 cut(s) 521, 855
XhoI CTCGAG 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.