Rh6CG353900

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0005281
Physical Location & Seq
Forward (+)
54286802 .. 54290110
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG353900.1

Sequence Viewer

Length: 1590 bp
ATGGAAGAGAAAAGAAGTTGGAACTTCAAAAACCCCAAAATCCTTAAATGCAAGTCAAAGCAACCCATCTTCTCTGACCCAATCACATATTCAAACCCTCTCTACTCTTCACACCCAACTGAAAGCTTCGCTGGGGGGTTTCACAATTCAAGCCCAGAATCACAACCCACGCAGATGACGGGGCTCGTAATGGTGACGAGCGGCGGCAGCGGTGGTTGCAAAGAGGGGAAGAAGAGCTCGGAAGAAGAGCAAAACCAGCTCTCTCTGGTGGATTTTCTGTTGGCCGCGGTGAGGAAATCCATGGTGGCCTGCCGCGTGGAGCGAGGCGAGGAGGTGATCTCCGCCGTCAATAATATGGAGATCGGGTGGCCCACGAATGTCCGGCATGTGACGCATGTCACGTTCGACCGGTTCAACGGGTTTCTGGGTCTTCCGGTGGAGTTTGAGGTCGATGTCCCGGGTCGGGTTCCGAGTGCTAGTGCAAGTGTGTTTGGGGTCTCAGCGGAGTCAATGCAGTTGTCTTTTGATTCAAAAGGGAATAGTGTCCCTACTATTCTCTTGCTGATGCAGGAGAGATTATACTCACAAGGAGGATTGAAGGCAGAAGGAATATTCCGTATAAACCCGGAGAACAGCAAAGAGGAGTTTGTGAGAAACCAGTTGAACAGAGGCATTGTGCCTGATGACATTGACGTCCATTGCTTGGCAGGCCTAATCAAAGCCTGGTTTCGAGAACTTCCTTCAGGAGTGCTAGATGGACTATCTCCTGAAGAAGTTCTTCAGTGCAACACAGAAGAGGAATCCTTTGAGCTTGTGAAGCAGTTAAAGCCAACCGAGGCTGCATTACTCAATTGGGCTATCAGTCTGATGGCTGATGTTGTTGAGGAAGAAGAATTTAACAAAATGAATGCAAGAAATATTGCTATGGTTTTTGCTCCAAACATGACTCAGATGTCTGATCCACTAACGGCTTTGATGCATGCGGTTCAAGTAATGAATTTGCTTAAGACCCTGATTATGAAAACACTAAGGGAACGTGAAGAGACTGATGAAACTGGAGGATATTCACCCATGTCATCTTGTTTATCTGATCATCAATCAGATGAAGACTTGTATAGTCATCCAGACATGGATACCAACGGAGACTTGTATAGTCATCCAGACATGGATACCAACTCTGAATTGAGAGGACTAACATCAGATTATGATGAAAATGCCCACTATAGCAACAGCAGTGAAGATGAAGATGAAGTTCGGCCACTCAGTGACATAGAAGAATGCTTTTTAAGACGATTGGATGAGAAGAAAACAGCCCCAAATAGCATGGCAATGCTGAATAAACCAACAAGTAAGTCACTTGGTGAGTATCCGAGTCCTCAAAGTTGCTCGAGCTTTAACATGGAATCTGGCACATCTTCTGTTGATAGCAAAAATGGGAATTCTGGCTTGTGTGCAAGTTATGGGGAAGACTCTGGAAAATGCACTGATATGGAGAGCCCTTCAGTATCATGCTCAACCTTGAAGGAAATGGATATGGAGGACAGAGTAATGGAGTCTGTTTCAACTAGGCCATTGTTTGCATCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

529

Amino Acids

58.53

Weight (kDa)

4.65

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 183 - 317 4.7e-20 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 692, 952
AatII GACGTC 1 cut(s) 696
AccB7I CCANNNNNTGG 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 201
AccII CGCG 2 cut(s) 287, 315
AciI CCGC 9 cut(s) 201, 204, 210, 285, 287, 313, 342, 503, 983
AclWI GGATC 1 cut(s) 953
AcoI YGGCCR 2 cut(s) 282, 1256
AcsI RAATTY 3 cut(s) 893, 997, 1438
AcuI CTGAAG 4 cut(s) 726, 764, 789, 1485
AcyI GRCGYC 1 cut(s) 693
AdeI CACNNNGTG 2 cut(s) 1265, 1361
AfiI CCNNNNNNNGG 3 cut(s) 291, 463, 703
AflII CTTAAG 1 cut(s) 1004
AgeI ACCGGT 1 cut(s) 408
AjnI CCWGG 1 cut(s) 722
AjuI GAANNNNNNNTTGG 2 cut(s) 73, 105
AluBI AGCT 5 cut(s) 126, 237, 259, 811, 1392
AluI AGCT 5 cut(s) 126, 237, 259, 811, 1392
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 3 cut(s) 502, 1037, 1137
AlwI GGATC 1 cut(s) 953
Ama87I CYCGRG 2 cut(s) 457, 1387
AoxI GGCC 6 cut(s) 282, 306, 368, 709, 1256, 1568
ApeKI GCWGC 2 cut(s) 207, 839
ApoI RAATTY 3 cut(s) 893, 997, 1438
AsiGI ACCGGT 1 cut(s) 408
Asp700I GAANNNNTTC 2 cut(s) 774, 777
AspS9I GGNCC 1 cut(s) 369
AsuC2I CCSGG 3 cut(s) 458, 459, 626
AsuHPI GGTGA 5 cut(s) 205, 301, 346, 1059, 1373
AvaI CYCGRG 2 cut(s) 457, 1387
BanII GRGCYC 3 cut(s) 186, 239, 1499
BbsI GAAGAC 3 cut(s) 422, 1113, 1473
Bbv12I GWGCWC 1 cut(s) 239
BbvI GCAGC 2 cut(s) 219, 826
BccI CCATC 3 cut(s) 74, 749, 862
BceAI ACGGC 2 cut(s) 329, 984
BciT130I CCWGG 1 cut(s) 724
BciVI GTATCC 3 cut(s) 1126, 1162, 1377
BclI TGATCA 1 cut(s) 1090
BcnI CCSGG 3 cut(s) 458, 459, 626
BcoDI GTCTC 3 cut(s) 502, 1037, 1137
BfaI CTAG 3 cut(s) 477, 752, 1566
BfmI CTRYAG 1 cut(s) 1222
BfrI CTTAAG 1 cut(s) 1004
BfuI GTATCC 3 cut(s) 1126, 1162, 1377
BisI GCNGC 6 cut(s) 202, 205, 208, 285, 313, 840
BlsI GCNGC 6 cut(s) 203, 206, 209, 286, 314, 841
Bme1390I CCNGG 4 cut(s) 458, 459, 626, 724
BmeT110I CYCGRG 2 cut(s) 457, 1387
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 4 cut(s) 458, 459, 626, 724
BmsI GCATC 2 cut(s) 555, 966
BoxI GACNNNNGTC 1 cut(s) 395
BpiI GAAGAC 3 cut(s) 422, 1113, 1473
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpmI CTGGAG 1 cut(s) 1077
BpuMI CCSGG 3 cut(s) 458, 459, 626
BsaHI GRCGYC 1 cut(s) 693
BsaI GGTCTC 1 cut(s) 502
BsaJI CCNNGG 4 cut(s) 285, 300, 457, 834
BsaWI WCCGGW 2 cut(s) 408, 433
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 463, 703
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 658, 1060
Bse3DI GCAATG 2 cut(s) 697, 1335
BseBI CCWGG 1 cut(s) 724
BseDI CCNNGG 4 cut(s) 285, 300, 457, 834
BseGI GGATG 3 cut(s) 1120, 1156, 1303
BseLI CCNNNNNNNGG 3 cut(s) 291, 463, 703
BseMI GCAATG 2 cut(s) 697, 1335
BseMII CTCAG 3 cut(s) 513, 962, 1276
BseNI ACTGG 2 cut(s) 658, 1060
BseRI GAGGAG 2 cut(s) 344, 656
BseXI GCAGC 2 cut(s) 219, 826
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 2 cut(s) 287, 315
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 6 cut(s) 284, 308, 370, 711, 1258, 1570
BshTI ACCGGT 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 239
BsiHKCI CYCGRG 2 cut(s) 457, 1387
BsiSI CCGG 5 cut(s) 382, 409, 434, 458, 626
BslFI GGGAC 2 cut(s) 440, 530
BslI CCNNNNNNNGG 3 cut(s) 291, 463, 703
BsmAI GTCTC 3 cut(s) 502, 1037, 1137
BsmFI GGGAC 2 cut(s) 440, 530
BsmI GAATGC 2 cut(s) 913, 1283
BsnI GGCC 6 cut(s) 284, 308, 370, 711, 1258, 1570
Bso31I GGTCTC 1 cut(s) 502
BsoBI CYCGRG 2 cut(s) 457, 1387
Bsp1286I GDGCHC 3 cut(s) 186, 239, 1499
Bsp143I GATC 4 cut(s) 336, 360, 958, 1090
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 9 cut(s) 201, 204, 210, 285, 287, 313, 342, 503, 983
BspANI GGCC 6 cut(s) 284, 308, 370, 711, 1258, 1570
BspCNI CTCAG 3 cut(s) 512, 961, 1275
BspFNI CGCG 2 cut(s) 287, 315
BspLI GGNNCC 1 cut(s) 468
BspPI GGATC 1 cut(s) 953
BspQI GCTCTTC 2 cut(s) 227, 240
BspTI CTTAAG 1 cut(s) 1004
BspTNI GGTCTC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 2 cut(s) 697, 1335
BsrFI RCCGGY 1 cut(s) 408
BsrI ACTGG 2 cut(s) 658, 1060
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 4 cut(s) 285, 300, 457, 834
BssMI GATC 4 cut(s) 336, 360, 958, 1090
BssNI GRCGYC 1 cut(s) 693
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 724
Bst6I CTCTTC 5 cut(s) 112, 227, 240, 789, 1035
BstACI GRCGYC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 1004
BstC8I GCNNGC 3 cut(s) 310, 709, 981
BstDEI CTNAG 4 cut(s) 499, 948, 1028, 1262
BstDSI CCRYGG 2 cut(s) 285, 300
BstF5I GGATG 3 cut(s) 1120, 1156, 1303
BstFNI CGCG 2 cut(s) 287, 315
BstKTI GATC 4 cut(s) 339, 363, 961, 1093
BstMAI GTCTC 3 cut(s) 502, 1037, 1137
BstMBI GATC 4 cut(s) 336, 360, 958, 1090
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 7 cut(s) 207, 216, 256, 391, 708, 817, 826
BstNI CCWGG 1 cut(s) 724
BstNSI RCATGY 3 cut(s) 389, 398, 983
BstPAI GACNNNNGTC 1 cut(s) 395
BstSCI CCNGG 4 cut(s) 456, 457, 624, 722
BstSFI CTRYAG 1 cut(s) 1222
BstUI CGCG 2 cut(s) 287, 315
BstV1I GCAGC 2 cut(s) 219, 826
BstV2I GAAGAC 3 cut(s) 422, 1113, 1473
BsuI GTATCC 3 cut(s) 1126, 1162, 1377
BsuRI GGCC 6 cut(s) 284, 308, 370, 711, 1258, 1570
BtgI CCRYGG 2 cut(s) 285, 300
BtsCI GGATG 3 cut(s) 1120, 1156, 1303
BtsI GCAGTG 1 cut(s) 1240
BtsIMutI CAGTG 4 cut(s) 788, 1240, 1270, 1482
Cac8I GCNNGC 3 cut(s) 310, 709, 981
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 369
Cfr42I CCGCGG 1 cut(s) 288
Cfr9I CCCGGG 1 cut(s) 457
CseI GACGC 1 cut(s) 400
CspAI ACCGGT 1 cut(s) 408
DdeI CTNAG 4 cut(s) 499, 948, 1028, 1262
DpnI GATC 4 cut(s) 338, 362, 960, 1092
DpnII GATC 4 cut(s) 336, 360, 958, 1090
DraIII CACNNNGTG 2 cut(s) 1265, 1361
DrdI GACNNNNNNGTC 2 cut(s) 692, 952
DseDI GACNNNNNNGTC 2 cut(s) 692, 952
EaeI YGGCCR 2 cut(s) 282, 1256
Eam1104I CTCTTC 5 cut(s) 112, 227, 240, 789, 1035
EarI CTCTTC 5 cut(s) 112, 227, 240, 789, 1035
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 711
Eco24I GRGCYC 3 cut(s) 186, 239, 1499
Eco31I GGTCTC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 237
Eco57I CTGAAG 4 cut(s) 726, 764, 789, 1485
Eco88I CYCGRG 2 cut(s) 457, 1387
EcoICRI GAGCTC 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 1438
EcoRII CCWGG 1 cut(s) 722
EcoT14I CCWWGG 1 cut(s) 300
EcoT22I ATGCAT 1 cut(s) 981
EcoT38I GRGCYC 3 cut(s) 186, 239, 1499
ErhI CCWWGG 1 cut(s) 300
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 2 cut(s) 440, 530
FbaI TGATCA 1 cut(s) 1090
Fnu4HI GCNGC 6 cut(s) 202, 205, 208, 285, 313, 840
FokI GGATG 3 cut(s) 1107, 1143, 1310
FriOI GRGCYC 3 cut(s) 186, 239, 1499
Fsp4HI GCNGC 6 cut(s) 202, 205, 208, 285, 313, 840
FspBI CTAG 3 cut(s) 477, 752, 1566
GluI GCNGC 6 cut(s) 202, 205, 208, 285, 313, 840
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 1077
HaeIII GGCC 6 cut(s) 284, 308, 370, 711, 1258, 1570
HapII CCGG 5 cut(s) 382, 409, 434, 458, 626
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 693
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 9 cut(s) 158, 506, 527, 800, 946, 1372, 1403, 1469, 1553
HpaII CCGG 5 cut(s) 382, 409, 434, 458, 626
HphI GGTGA 5 cut(s) 205, 301, 346, 1059, 1373
Hpy188III TCNNGA 6 cut(s) 731, 744, 767, 1124, 1160, 1473
HpyAV CCTTC 5 cut(s) 592, 599, 750, 1509, 1516
HpyCH4IV ACGT 3 cut(s) 401, 693, 1036
HpyF10VI GCNNNNNNNGC 7 cut(s) 207, 216, 256, 391, 708, 817, 826
HpyF3I CTNAG 4 cut(s) 499, 948, 1028, 1262
HpySE526I ACGT 3 cut(s) 401, 693, 1036
Hsp92I GRCGYC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 1090
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 4 cut(s) 336, 360, 958, 1090
LguI GCTCTTC 2 cut(s) 227, 240
LmnI GCTCC 2 cut(s) 319, 940
Lsp1109I GCAGC 2 cut(s) 219, 826
LweI GCATC 2 cut(s) 555, 966
MaeI CTAG 3 cut(s) 477, 752, 1566
MaeII ACGT 3 cut(s) 401, 693, 1036
MaeIII GTNAC 5 cut(s) 193, 388, 397, 1265, 1353
MalI GATC 4 cut(s) 338, 362, 960, 1092
MbiI CCGCTC 1 cut(s) 201
MboI GATC 4 cut(s) 336, 360, 958, 1090
MfeI CAATTG 1 cut(s) 850
MhlI GDGCHC 3 cut(s) 186, 239, 1499
MluCI AATT 6 cut(s) 145, 850, 893, 997, 1181, 1438
MlyI GAGTC 5 cut(s) 515, 940, 1381, 1463, 1562
Mph1103I ATGCAT 1 cut(s) 981
MroXI GAANNNNTTC 2 cut(s) 774, 777
MseI TTAA 7 cut(s) 45, 824, 897, 1005, 1286, 1395, 1588
MslI CAYNNNNRTG 3 cut(s) 173, 1328, 1487
MspA1I CMGCKG 3 cut(s) 210, 287, 503
MspCI CTTAAG 1 cut(s) 1004
MspI CCGG 5 cut(s) 382, 409, 434, 458, 626
MspR9I CCNGG 4 cut(s) 458, 459, 626, 724
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 2 cut(s) 913, 1283
MvaI CCWGG 1 cut(s) 724
MvnI CGCG 2 cut(s) 287, 315
MwoI GCNNNNNNNGC 7 cut(s) 207, 216, 256, 391, 708, 817, 826
NciI CCSGG 3 cut(s) 458, 459, 626
NcoI CCATGG 1 cut(s) 300
NdeII GATC 4 cut(s) 336, 360, 958, 1090
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 5 cut(s) 193, 388, 397, 1265, 1353
NsiI ATGCAT 1 cut(s) 981
NspI RCATGY 3 cut(s) 389, 398, 983
PaeI GCATGC 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 1387
PceI AGGCCT 1 cut(s) 711
PciSI GCTCTTC 2 cut(s) 227, 240
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 2 cut(s) 913, 1283
PdmI GAANNNNTTC 2 cut(s) 774, 777
PfeI GAWTC 4 cut(s) 158, 527, 800, 1403
PflMI CCANNNNNTGG 1 cut(s) 703
PinAI ACCGGT 1 cut(s) 408
PkrI GCNGC 6 cut(s) 203, 206, 209, 286, 314, 841
PleI GAGTC 5 cut(s) 514, 940, 1380, 1463, 1561
PpsI GAGTC 5 cut(s) 514, 940, 1380, 1463, 1561
PshAI GACNNNNGTC 1 cut(s) 395
Psp124BI GAGCTC 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 1 cut(s) 722
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 369
PspXI VCTCGAGB 1 cut(s) 1387
RseI CAYNNNNRTG 3 cut(s) 173, 1328, 1487
SacI GAGCTC 1 cut(s) 239
SacII CCGCGG 1 cut(s) 288
SapI GCTCTTC 2 cut(s) 227, 240
SaqAI TTAA 7 cut(s) 45, 824, 897, 1005, 1286, 1395, 1588
SatI GCNGC 6 cut(s) 202, 205, 208, 285, 313, 840
Sau3AI GATC 4 cut(s) 336, 360, 958, 1090
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 5 cut(s) 515, 940, 1381, 1463, 1562
ScrFI CCNGG 4 cut(s) 458, 459, 626, 724
SduI GDGCHC 3 cut(s) 186, 239, 1499
SfaNI GCATC 2 cut(s) 555, 966
SfcI CTRYAG 1 cut(s) 1222
Sfr274I CTCGAG 1 cut(s) 1387
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SlaI CTCGAG 1 cut(s) 1387
SmaI CCCGGG 1 cut(s) 459
SmiMI CAYNNNNRTG 3 cut(s) 173, 1328, 1487
SmlI CTYRAG 2 cut(s) 1004, 1387
SmoI CTYRAG 2 cut(s) 1004, 1387
SphI GCATGC 1 cut(s) 983
Sse9I AATT 6 cut(s) 145, 850, 893, 997, 1181, 1438
SseBI AGGCCT 1 cut(s) 711
SsiI CCGC 9 cut(s) 201, 204, 210, 285, 287, 313, 342, 503, 983
SspI AATATT 2 cut(s) 612, 919
SspMI CTAG 3 cut(s) 477, 752, 1566
SstI GAGCTC 1 cut(s) 239
StuI AGGCCT 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 456, 457, 624, 722
StyI CCWWGG 1 cut(s) 300
TaiI ACGT 3 cut(s) 404, 696, 1039
TaqI TCGA 4 cut(s) 405, 450, 730, 1388
TasI AATT 6 cut(s) 145, 850, 893, 997, 1181, 1438
TauI GCSGC 4 cut(s) 204, 207, 287, 315
TfiI GAWTC 4 cut(s) 158, 527, 800, 1403
Tru1I TTAA 7 cut(s) 45, 824, 897, 1005, 1286, 1395, 1588
Tru9I TTAA 7 cut(s) 45, 824, 897, 1005, 1286, 1395, 1588
TscAI CASTG 4 cut(s) 788, 1240, 1270, 1489
TseFI GTSAC 5 cut(s) 193, 388, 397, 1265, 1353
TseI GCWGC 2 cut(s) 207, 839
Tsp45I GTSAC 5 cut(s) 193, 388, 397, 1265, 1353
TspDTI ATGAA 8 cut(s) 920, 1010, 1034, 1065, 1119, 1224, 1257, 1263
TspGWI ACGGA 2 cut(s) 605, 1155
TspMI CCCGGG 1 cut(s) 457
TspRI CASTG 4 cut(s) 788, 1240, 1270, 1489
Van91I CCANNNNNTGG 1 cut(s) 703
Vha464I CTTAAG 1 cut(s) 1004
XapI RAATTY 3 cut(s) 893, 997, 1438
XceI RCATGY 3 cut(s) 389, 398, 983
XhoI CTCGAG 1 cut(s) 1387
XmaI CCCGGG 1 cut(s) 457
XmnI GAANNNNTTC 2 cut(s) 774, 777
XspI CTAG 3 cut(s) 477, 752, 1566
ZraI GACGTC 1 cut(s) 694
Zsp2I ATGCAT 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.