AT4G15093

catalytic LigB subunit of aromatic ring-opening dioxygenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005435
Physical Location & Seq
Forward (+)
8618259 .. 8619658
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G15093.1

Sequence Viewer

Length: 810 bp
ATGGAGAAAGTGAATCAAACCTTCTTCTTATCTCATGGATCTCCAACTTTGAGCATAGATGATTCCTTGGAAGCGAGACAATTCTTCAAATCATGGACTCAAAAAGTTCTTCCTCAAAAACCCAAATCGATTTTAGTCATCTCTGCTCATTGGGACACCAAATTCCCATCTGTCAACACTGTTCTTCGCAATAACACGATCCACGATTTCTCTGGCTTCCCTGATCCTATGTACAAGTTGAAATATGAAGCACCAGGAGCAATTGAATTAGGGAAAAGGGTTAAAGAGTTGTTAATGAAAGAAGGAGGAATGAAACGTGTTGATGAAGATACAAAGAGAGGACTTGATCATGGAGCTTGGGTTCCTCTTATGTTGATGTATCCAGAAGCTGATATACCCATTTGTCAACTCTCTGTTCAATCAAATCAAAATGGGAGTTATCATTACAACATGGGTAAAGCATTGGCTTCATTGAAAGATGAAGGTGTTCTTATCATTGGCTCTGGAAGTGCTACTCATAACTTGAGGAAGCTTGACTTTAATATCACTGATGGCTCACCCGTTCCTTGGGCCTTGGAGTTTGATCATTGGCTTAGAGATTCTCTACTTCAAGGAAGGTATGGAGATGTGAATGAGTGGGAAGAGAAAGCTCCGAATGCGAAAATGGCGCATCCGTGGCCCGAGCATTTGTATCCGTTACATGTTGTGATGGGTGCAGCAGGTGGAGATGCAAAGGCAGAGCAAATCCATACAAGTTGGCAACTTGGTACCTTGTCTTATTCTTCTTATAGCTTCACTTCTTCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

269

Amino Acids

30.25

Weight (kDa)

6.25

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 7 - 265 1.4e-50 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 710
Acc65I GGTACC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 767
AclWI GGATC 3 cut(s) 46, 193, 218
AcsI RAATTY 1 cut(s) 161
AfaI GTAC 2 cut(s) 233, 769
AfiI CCNNNNNNNGG 1 cut(s) 567
AflIII ACRYGT 2 cut(s) 316, 700
AgsI TTSAA 6 cut(s) 88, 241, 266, 419, 475, 611
AjnI CCWGG 1 cut(s) 253
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 5 cut(s) 356, 389, 532, 650, 792
AluI AGCT 5 cut(s) 356, 389, 532, 650, 792
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 3 cut(s) 46, 193, 218
AlwNI CAGNNNCTG 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 2 cut(s) 570, 677
ApeKI GCWGC 1 cut(s) 716
ApoI RAATTY 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 486
Asp718I GGTACC 1 cut(s) 767
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 570, 678
AsuHPI GGTGA 1 cut(s) 549
AvaI CYCGRG 1 cut(s) 680
BanI GGYRCC 1 cut(s) 767
BarI GAAGNNNNNNTAC 2 cut(s) 378, 410
BbvI GCAGC 1 cut(s) 728
BccI CCATC 3 cut(s) 175, 545, 703
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 2 cut(s) 390, 702
BclI TGATCA 2 cut(s) 346, 583
BcoDI GTCTC 1 cut(s) 70
BfuAI ACCTGC 1 cut(s) 710
BfuI GTATCC 2 cut(s) 390, 702
BisI GCNGC 1 cut(s) 717
BlsI GCNGC 1 cut(s) 718
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 2 cut(s) 570, 678
BmiI GGNNCC 2 cut(s) 363, 769
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 679, 718
BpuEI CTTGAG 1 cut(s) 544
Bsa29I ATCGAT 1 cut(s) 128
BsaJI CCNNGG 4 cut(s) 66, 566, 573, 674
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 255
BseCI ATCGAT 1 cut(s) 128
BseDI CCNNGG 4 cut(s) 66, 566, 573, 674
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 1 cut(s) 567
BseXI GCAGC 1 cut(s) 728
BsgI GTGCAG 1 cut(s) 735
BshFI GGCC 2 cut(s) 572, 679
BshNI GGYRCC 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 128
BsiHKCI CYCGRG 1 cut(s) 680
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 567
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 661
BsnI GGCC 2 cut(s) 572, 679
BsoBI CYCGRG 1 cut(s) 680
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 5 cut(s) 38, 198, 223, 346, 583
BspANI GGCC 2 cut(s) 572, 679
BspDI ATCGAT 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 363, 769
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 3 cut(s) 46, 193, 218
BspT107I GGYRCC 1 cut(s) 767
BsrGI TGTACA 1 cut(s) 231
BssECI CCNNGG 4 cut(s) 66, 566, 573, 674
BssMI GATC 5 cut(s) 38, 198, 223, 346, 583
BssT1I CCWWGG 3 cut(s) 66, 566, 573
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 636
BstAUI TGTACA 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 593
BstDSI CCRYGG 1 cut(s) 674
BstF5I GGATG 1 cut(s) 670
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 5 cut(s) 41, 201, 226, 349, 586
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 5 cut(s) 38, 198, 223, 346, 583
BstMWI GCNNNNNNNGC 4 cut(s) 257, 656, 665, 676
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 728
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 128
BsuI GTATCC 2 cut(s) 390, 702
BsuRI GGCC 2 cut(s) 572, 679
BsuTUI ATCGAT 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 674
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 2 cut(s) 177, 546
BveI ACCTGC 1 cut(s) 710
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 570, 678
ClaI ATCGAT 1 cut(s) 128
Csp6I GTAC 2 cut(s) 232, 768
CviAII CATG 5 cut(s) 35, 93, 350, 451, 701
CviQI GTAC 2 cut(s) 232, 768
DdeI CTNAG 1 cut(s) 593
DpnI GATC 5 cut(s) 40, 200, 225, 348, 585
DpnII GATC 5 cut(s) 38, 198, 223, 346, 583
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
Eco130I CCWWGG 3 cut(s) 66, 566, 573
Eco88I CYCGRG 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 66, 566, 573
ErhI CCWWGG 3 cut(s) 66, 566, 573
FaeI CATG 5 cut(s) 38, 96, 353, 454, 704
FalI AAGNNNNNCTT 4 cut(s) 474, 506, 521, 553
FaqI GGGAC 1 cut(s) 167
FatI CATG 5 cut(s) 34, 92, 349, 450, 700
FbaI TGATCA 2 cut(s) 346, 583
Fnu4HI GCNGC 1 cut(s) 717
FokI GGATG 1 cut(s) 657
Fsp4HI GCNGC 1 cut(s) 717
GlaI GCGC 1 cut(s) 669
GluI GCNGC 1 cut(s) 717
HaeIII GGCC 2 cut(s) 572, 679
HhaI GCGC 1 cut(s) 670
Hin1II CATG 5 cut(s) 38, 96, 353, 454, 704
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 2 cut(s) 175, 407
HindII GTYRAC 2 cut(s) 175, 407
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 4 cut(s) 13, 62, 97, 599
HphI GGTGA 1 cut(s) 549
Hpy166II GTNNAC 2 cut(s) 175, 407
Hpy188I TCNGA 1 cut(s) 654
Hpy188III TCNNGA 2 cut(s) 383, 504
Hpy8I GTNNAC 2 cut(s) 175, 407
HpyAV CCTTC 4 cut(s) 31, 296, 476, 609
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 2 cut(s) 716, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 656, 665, 676
HpyF3I CTNAG 1 cut(s) 593
HpySE526I ACGT 1 cut(s) 316
Hsp92II CATG 5 cut(s) 38, 96, 353, 454, 704
HspAI GCGC 1 cut(s) 668
KpnI GGTACC 1 cut(s) 771
Ksp22I TGATCA 2 cut(s) 346, 583
Kzo9I GATC 5 cut(s) 38, 198, 223, 346, 583
LmnI GCTCC 3 cut(s) 257, 353, 655
LpnPI CCDG 7 cut(s) 198, 234, 240, 267, 396, 489, 705
Lsp1109I GCAGC 1 cut(s) 728
LweI GCATC 2 cut(s) 679, 718
MaeII ACGT 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 696
MalI GATC 5 cut(s) 40, 200, 225, 348, 585
MboI GATC 5 cut(s) 38, 198, 223, 346, 583
MboII GAAGA 8 cut(s) 16, 76, 101, 176, 338, 653, 774, 792
MfeI CAATTG 1 cut(s) 261
MflI RGATCY 1 cut(s) 38
MluCI AATT 4 cut(s) 80, 161, 261, 266
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 5 cut(s) 123, 299, 332, 375, 519
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 3 cut(s) 282, 293, 540
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 661
MvaI CCWGG 1 cut(s) 255
MwoI GCNNNNNNNGC 4 cut(s) 257, 656, 665, 676
NdeII GATC 5 cut(s) 38, 198, 223, 346, 583
NlaIII CATG 5 cut(s) 38, 96, 353, 454, 704
NlaIV GGNNCC 2 cut(s) 363, 769
NspI RCATGY 1 cut(s) 704
PaqCI CACCTGC 1 cut(s) 710
PciI ACATGT 1 cut(s) 700
PctI GAATGC 1 cut(s) 661
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 13, 62, 599
PkrI GCNGC 1 cut(s) 718
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PscI ACATGT 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 363, 769
PspPI GGNCC 2 cut(s) 570, 678
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 38
RsaI GTAC 2 cut(s) 233, 769
RsaNI GTAC 2 cut(s) 232, 768
SaqAI TTAA 3 cut(s) 282, 293, 540
SatI GCNGC 1 cut(s) 717
Sau3AI GATC 5 cut(s) 38, 198, 223, 346, 583
Sau96I GGNCC 2 cut(s) 570, 678
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 1 cut(s) 255
SfaNI GCATC 2 cut(s) 679, 718
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
Sse9I AATT 4 cut(s) 80, 161, 261, 266
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 3 cut(s) 66, 566, 573
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 128
TasI AATT 4 cut(s) 80, 161, 261, 266
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 3 cut(s) 13, 62, 599
Tru1I TTAA 3 cut(s) 282, 293, 540
Tru9I TTAA 3 cut(s) 282, 293, 540
TscAI CASTG 2 cut(s) 184, 553
TseI GCWGC 1 cut(s) 716
TspDTI ATGAA 6 cut(s) 261, 311, 326, 339, 459, 495
TspGWI ACGGA 2 cut(s) 663, 684
TspRI CASTG 2 cut(s) 184, 553
XapI RAATTY 1 cut(s) 161
XceI RCATGY 1 cut(s) 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.