Rh2BG293300

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005435
Physical Location & Seq
Reverse (-)
33074652 .. 33074969
318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG293300.1

Sequence Viewer

Length: 318 bp
ATGCCGATAATAGACACATTCTACATGTTGCACGGATCCCCAACTCTAAGCATCGATGAGACCATACCCGCCCGAAAGTTTCTCCAATCTTGGAAGCAAAACGGGTTCCCCCAAAAACCCAACTCTATCCTCACCATCTCTACGCACTGGGAAGCCTCCATCCCCATATTTAACTCTATTGTCGGCCTAAACGAGACCATCTACTACTTCTACAGCTTTCCCAACTCCCTGTACAAGCTCAACTACCCTGCCCCAAGGTCACCCCAACTACCCGGAGGCAGACATCCTGGTGTTCCAGCTGTCCGTCGTGACCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

105

Amino Acids

12.01

Weight (kDa)

9.63

Isoelectric Point (pI)

69.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 69
AclWI GGATC 2 cut(s) 30, 43
AfaI GTAC 1 cut(s) 233
AflIII ACRYGT 1 cut(s) 24
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 3 cut(s) 216, 238, 299
AluI AGCT 3 cut(s) 216, 238, 299
Alw26I GTCTC 2 cut(s) 53, 188
AlwI GGATC 2 cut(s) 30, 43
AoxI GGCC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 124, 252
BamHI GGATCC 1 cut(s) 35
BccI CCATC 3 cut(s) 143, 167, 206
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 53, 188
BfmI CTRYAG 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 273, 288
BmiI GGNNCC 2 cut(s) 37, 107
BmrFI CCNGG 2 cut(s) 273, 288
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 157
BpuMI CCSGG 1 cut(s) 273
Bsa29I ATCGAT 1 cut(s) 54
BsaI GGTCTC 2 cut(s) 53, 188
BsaJI CCNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 288
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 2 cut(s) 159, 283
BseNI ACTGG 1 cut(s) 152
BshFI GGCC 1 cut(s) 186
BshVI ATCGAT 1 cut(s) 54
BsiSI CCGG 1 cut(s) 273
BsmAI GTCTC 2 cut(s) 53, 188
BsnI GGCC 1 cut(s) 186
Bso31I GGTCTC 2 cut(s) 53, 188
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 1 cut(s) 35
BspACI CCGC 1 cut(s) 69
BspANI GGCC 1 cut(s) 186
BspDI ATCGAT 1 cut(s) 54
BspLI GGNNCC 2 cut(s) 37, 107
BspPI GGATC 2 cut(s) 30, 43
BspTNI GGTCTC 2 cut(s) 53, 188
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 258
BstF5I GGATG 2 cut(s) 159, 283
BstKTI GATC 1 cut(s) 38
BstMAI GTCTC 2 cut(s) 53, 188
BstMBI GATC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 28
BstPI GGTNACC 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 271, 286
BstSFI CTRYAG 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
Bsu15I ATCGAT 1 cut(s) 54
BsuRI GGCC 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 54
BtsCI GGATG 2 cut(s) 159, 283
BtsIMutI CAGTG 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 54
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 25
CviJI RGCY 5 cut(s) 155, 186, 216, 238, 299
CviKI_1 RGCY 5 cut(s) 155, 186, 216, 238, 299
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 1 cut(s) 47
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 254
Eco31I GGTCTC 2 cut(s) 53, 188
Eco91I GGTNACC 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 1 cut(s) 28
FaiI YATR 3 cut(s) 26, 65, 167
FatI CATG 1 cut(s) 24
FauI CCCGC 1 cut(s) 76
FokI GGATG 2 cut(s) 146, 270
HaeIII GGCC 1 cut(s) 186
HapII CCGG 1 cut(s) 273
Hin1II CATG 1 cut(s) 28
HpaII CCGG 1 cut(s) 273
HphI GGTGA 2 cut(s) 124, 252
Hpy188III TCNNGA 1 cut(s) 308
Hpy99I CGWCG 1 cut(s) 309
HpyCH4V TGCA 1 cut(s) 31
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 1 cut(s) 28
Kzo9I GATC 1 cut(s) 35
LpnPI CCDG 7 cut(s) 133, 242, 261, 273, 286, 300, 309
LweI GCATC 1 cut(s) 60
MaeIII GTNAC 2 cut(s) 258, 308
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MflI RGATCY 1 cut(s) 35
MnlI CCTC 3 cut(s) 140, 166, 269
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 2 cut(s) 273, 288
MvaI CCWGG 1 cut(s) 288
NciI CCSGG 1 cut(s) 273
NdeII GATC 1 cut(s) 35
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 2 cut(s) 37, 107
NmuCI GTSAC 2 cut(s) 258, 308
NspI RCATGY 1 cut(s) 28
PciI ACATGT 1 cut(s) 24
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PscI ACATGT 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 286
PspEI GGTNACC 1 cut(s) 258
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 2 cut(s) 37, 107
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 288
SaqAI TTAA 1 cut(s) 171
Sau3AI GATC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 273, 288
SetI ASST 4 cut(s) 218, 240, 260, 301
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 288
SsiI CCGC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 271, 286
StyI CCWWGG 1 cut(s) 254
TaqI TCGA 1 cut(s) 54
TatI WGTACW 1 cut(s) 231
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 2 cut(s) 258, 308
Tsp45I GTSAC 2 cut(s) 258, 308
TspGWI ACGGA 2 cut(s) 48, 293
TspRI CASTG 1 cut(s) 152
XceI RCATGY 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.