RLG00000014556

4,5-DOPA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005435
Physical Location & Seq
Reverse (-)
54634225 .. 54635210
986 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014556

Sequence Viewer

Length: 732 bp
ATGACAGAAGCACATACTTCCTCCGATCCTGGAAACAAAACGGGTTCCCCCAGAAACCCAACTCCATCCTCGTCATCTCCGGCCACTGGGAAACCTCCGTCCCCACCGTCAACTCCATCGCCGGCCCACACGACACCATCTACGACTTCTACGGCTTCCCCAAACCCATGCAAGCTCAAGTACCCCGCCCCCGGGTCCCCCCAGCTCGCCTCCCGCGTCAAGGACCTCCTCACCGCCGCCGGGTTCCCCCGCGTCGAAGTGGACACCAAGCGCGGCCTCGACCACGGCGCGTGGGTCCCGCTCATGTTCATGTACCCGGAGGCGGATGTTCCGGTGTGCCAGCTGTCCGTCCAGACTAACAGAGACGGGAGCTATCACTTCGATATGGGGAAGGCGCTGGCGCCGTTGAGGGAGGAGGGTGTTCTTATTGTGGGGTCGGGGAGCGCGACGCATAATCTAAGGGCGCTAAGGAACACGGACGAGGCGGTGGCGCCGTGGGCGTTGGAGTTTGATACTTGGCTTAAGGAGGCGTTGACTCAAGGAAGATATGAAGACGTGAACCATTATGAGGAGAAAGCTCCGCATGCGAAAACGGCTCATCCGTGGCCTGACCATTTCTACCCTCTTCATGTGGCAATGGGGGCTGCCGGTGCGAATTCGAAGGCCAAGCTCATCCATGAAAGCTGGCAGCTTGGTACTCTTTCTTATGCTTCATACCAGCTTACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

244

Amino Acids

26.15

Weight (kDa)

6.59

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 58 - 241 8.3e-41 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 400, 490
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 5 cut(s) 216, 252, 273, 290, 446
AclWI GGATC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 655
AcyI GRCGYC 2 cut(s) 401, 491
AfaI GTAC 3 cut(s) 182, 314, 697
AfiI CCNNNNNNNGG 7 cut(s) 86, 191, 192, 220, 240, 322, 568
AflII CTTAAG 1 cut(s) 521
AhlI ACTAGT 1 cut(s) 725
AjiI CACGTC 1 cut(s) 556
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 9 cut(s) 175, 205, 343, 372, 578, 670, 684, 691, 721
AluI AGCT 9 cut(s) 175, 205, 343, 372, 578, 670, 684, 691, 721
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 1 cut(s) 20
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 5 cut(s) 81, 123, 274, 605, 663
ApeKI GCWGC 2 cut(s) 644, 688
ApoI RAATTY 1 cut(s) 655
AspLEI GCGC 7 cut(s) 273, 290, 397, 403, 446, 466, 493
AspS9I GGNCC 4 cut(s) 124, 195, 223, 295
AsuC2I CCSGG 4 cut(s) 192, 193, 241, 317
AsuHPI GGTGA 1 cut(s) 223
AsuII TTCGAA 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 3 cut(s) 195, 223, 295
BanI GGYRCC 2 cut(s) 400, 490
BbsI GAAGAC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 631, 700
BccI CCATC 3 cut(s) 73, 124, 145
BceAI ACGGC 5 cut(s) 168, 301, 388, 478, 609
BciT130I CCWGG 1 cut(s) 30
BcnI CCSGG 4 cut(s) 192, 193, 241, 317
BcoDI GTCTC 1 cut(s) 357
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 1 cut(s) 726
BfoI RGCGCY 4 cut(s) 398, 404, 467, 494
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 4 cut(s) 237, 274, 645, 689
BlsI GCNGC 4 cut(s) 238, 275, 646, 690
Bme1390I CCNGG 5 cut(s) 30, 192, 193, 241, 317
Bme18I GGWCC 3 cut(s) 195, 223, 295
BmeT110I CYCGRG 1 cut(s) 191
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 4 cut(s) 124, 195, 223, 295
BmiI GGNNCC 8 cut(s) 46, 196, 197, 245, 296, 297, 402, 492
BmrFI CCNGG 5 cut(s) 30, 192, 193, 241, 317
BmrI ACTGGG 1 cut(s) 96
BmuI ACTGGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 558
Bpu10I CCTNAGC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 2 cut(s) 161, 522
BpuMI CCSGG 4 cut(s) 192, 193, 241, 317
BsaHI GRCGYC 2 cut(s) 401, 491
BsaJI CCNNGG 5 cut(s) 190, 191, 283, 494, 602
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 7 cut(s) 86, 191, 192, 220, 240, 322, 568
Bse118I RCCGGY 2 cut(s) 121, 647
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 5 cut(s) 190, 191, 283, 494, 602
BseGI GGATG 4 cut(s) 65, 331, 598, 672
BseLI CCNNNNNNNGG 7 cut(s) 86, 191, 192, 220, 240, 322, 568
BseMI GCAATG 1 cut(s) 642
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 3 cut(s) 218, 428, 584
BseXI GCAGC 2 cut(s) 631, 700
BseYI CCCAGC 1 cut(s) 201
Bsh1236I CGCG 5 cut(s) 216, 252, 273, 290, 446
BshFI GGCC 5 cut(s) 83, 125, 276, 607, 665
BshNI GGYRCC 2 cut(s) 400, 490
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 7 cut(s) 80, 122, 192, 240, 317, 332, 648
BslFI GGGAC 3 cut(s) 85, 181, 281
BslI CCNNNNNNNGG 7 cut(s) 86, 191, 192, 220, 240, 322, 568
BsmAI GTCTC 1 cut(s) 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 3 cut(s) 85, 181, 281
BsnI GGCC 5 cut(s) 83, 125, 276, 607, 665
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 659
Bsp143I GATC 1 cut(s) 25
BspANI GGCC 5 cut(s) 83, 125, 276, 607, 665
BspFNI CGCG 5 cut(s) 216, 252, 273, 290, 446
BspLI GGNNCC 8 cut(s) 46, 196, 197, 245, 296, 297, 402, 492
BspPI GGATC 1 cut(s) 20
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 2 cut(s) 400, 490
BspTI CTTAAG 1 cut(s) 521
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 2 cut(s) 121, 647
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 2 cut(s) 121, 647
BssECI CCNNGG 5 cut(s) 190, 191, 283, 494, 602
BssMI GATC 1 cut(s) 25
BssNI GRCGYC 2 cut(s) 401, 491
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 630
BstACI GRCGYC 2 cut(s) 401, 491
BstAFI CTTAAG 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 7 cut(s) 123, 173, 207, 341, 399, 585, 686
BstDEI CTNAG 2 cut(s) 458, 467
BstDSI CCRYGG 3 cut(s) 283, 494, 602
BstF5I GGATG 4 cut(s) 65, 331, 598, 672
BstFNI CGCG 5 cut(s) 216, 252, 273, 290, 446
BstH2I RGCGCY 4 cut(s) 398, 404, 467, 494
BstHHI GCGC 7 cut(s) 273, 290, 397, 403, 446, 466, 493
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 1 cut(s) 25
BstMWI GCNNNNNNNGC 5 cut(s) 497, 584, 593, 641, 650
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 5 cut(s) 28, 190, 191, 239, 315
BstUI CGCG 5 cut(s) 216, 252, 273, 290, 446
BstV1I GCAGC 2 cut(s) 631, 700
BstV2I GAAGAC 1 cut(s) 558
BsuRI GGCC 5 cut(s) 83, 125, 276, 607, 665
BtgI CCRYGG 3 cut(s) 283, 494, 602
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 4 cut(s) 65, 331, 598, 672
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 7 cut(s) 123, 173, 207, 341, 399, 585, 686
CfoI GCGC 7 cut(s) 273, 290, 397, 403, 446, 466, 493
Cfr10I RCCGGY 2 cut(s) 121, 647
Cfr13I GGNCC 4 cut(s) 124, 195, 223, 295
Cfr9I CCCGGG 1 cut(s) 191
CseI GACGC 3 cut(s) 205, 241, 457
Csp6I GTAC 3 cut(s) 181, 313, 696
CviAII CATG 6 cut(s) 168, 304, 310, 584, 629, 677
CviQI GTAC 3 cut(s) 181, 313, 696
DdeI CTNAG 2 cut(s) 458, 467
DinI GGCGCC 2 cut(s) 402, 492
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 338
Eco47I GGWCC 3 cut(s) 195, 223, 295
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 3 cut(s) 195, 223, 295
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 28
EgeI GGCGCC 2 cut(s) 402, 492
EheI GGCGCC 2 cut(s) 402, 492
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 6 cut(s) 171, 307, 313, 587, 632, 680
FaqI GGGAC 3 cut(s) 85, 181, 281
FatI CATG 6 cut(s) 167, 303, 309, 583, 628, 676
FauI CCCGC 4 cut(s) 193, 221, 257, 306
Fnu4HI GCNGC 4 cut(s) 237, 274, 645, 689
FokI GGATG 4 cut(s) 52, 338, 585, 659
Fsp4HI GCNGC 4 cut(s) 237, 274, 645, 689
FspBI CTAG 1 cut(s) 726
GlaI GCGC 7 cut(s) 272, 289, 396, 402, 445, 465, 492
GluI GCNGC 4 cut(s) 237, 274, 645, 689
GsaI CCCAGC 1 cut(s) 205
HaeII RGCGCY 4 cut(s) 398, 404, 467, 494
HaeIII GGCC 5 cut(s) 83, 125, 276, 607, 665
HapII CCGG 7 cut(s) 80, 122, 192, 240, 317, 332, 648
HgaI GACGC 3 cut(s) 205, 241, 457
HhaI GCGC 7 cut(s) 273, 290, 397, 403, 446, 466, 493
Hin1I GRCGYC 2 cut(s) 401, 491
Hin1II CATG 6 cut(s) 171, 307, 313, 587, 632, 680
Hin6I GCGC 7 cut(s) 271, 288, 395, 401, 444, 464, 491
HinP1I GCGC 7 cut(s) 271, 288, 395, 401, 444, 464, 491
HincII GTYRAC 2 cut(s) 111, 534
HindII GTYRAC 2 cut(s) 111, 534
HinfI GANTC 1 cut(s) 535
HpaII CCGG 7 cut(s) 80, 122, 192, 240, 317, 332, 648
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 4 cut(s) 111, 262, 534, 559
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 4 cut(s) 111, 262, 534, 559
Hpy99I CGWCG 2 cut(s) 257, 451
HpyAV CCTTC 2 cut(s) 385, 655
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 5 cut(s) 497, 584, 593, 641, 650
HpyF3I CTNAG 2 cut(s) 458, 467
HpySE526I ACGT 1 cut(s) 555
Hsp92I GRCGYC 2 cut(s) 401, 491
Hsp92II CATG 6 cut(s) 171, 307, 313, 587, 632, 680
HspAI GCGC 7 cut(s) 271, 288, 395, 401, 444, 464, 491
KasI GGCGCC 2 cut(s) 400, 490
KflI GGGWCCC 2 cut(s) 195, 295
KroI GCCGGC 1 cut(s) 121
KroNI GCCGGC 1 cut(s) 123
Kzo9I GATC 1 cut(s) 25
LmnI GCTCC 3 cut(s) 369, 441, 583
Lsp1109I GCAGC 2 cut(s) 631, 700
MaeI CTAG 1 cut(s) 726
MaeII ACGT 1 cut(s) 555
MalI GATC 1 cut(s) 27
MbiI CCGCTC 1 cut(s) 301
MboI GATC 1 cut(s) 25
MboII GAAGA 3 cut(s) 555, 563, 617
MluCI AATT 1 cut(s) 655
Mly113I GGCGCC 2 cut(s) 401, 491
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 1 cut(s) 483
MroNI GCCGGC 1 cut(s) 121
MseI TTAA 1 cut(s) 522
MslI CAYNNNNRTG 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 343
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 7 cut(s) 80, 122, 192, 240, 317, 332, 648
MspR9I CCNGG 5 cut(s) 30, 192, 193, 241, 317
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 5 cut(s) 216, 252, 273, 290, 446
MwoI GCNNNNNNNGC 5 cut(s) 497, 584, 593, 641, 650
NaeI GCCGGC 1 cut(s) 123
NarI GGCGCC 2 cut(s) 401, 491
NciI CCSGG 4 cut(s) 192, 193, 241, 317
NdeII GATC 1 cut(s) 25
NgoMIV GCCGGC 1 cut(s) 121
NlaIII CATG 6 cut(s) 171, 307, 313, 587, 632, 680
NlaIV GGNNCC 8 cut(s) 46, 196, 197, 245, 296, 297, 402, 492
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 1 cut(s) 659
PaeI GCATGC 1 cut(s) 587
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 443, 599
PdiI GCCGGC 1 cut(s) 123
PfoI TCCNGGA 1 cut(s) 28
PkrI GCNGC 4 cut(s) 238, 275, 646, 690
PleI GAGTC 1 cut(s) 529
PluTI GGCGCC 2 cut(s) 404, 494
PpsI GAGTC 1 cut(s) 529
PpuMI RGGWCCY 3 cut(s) 195, 223, 295
Psp5II RGGWCCY 3 cut(s) 195, 223, 295
Psp6I CCWGG 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 201
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 8 cut(s) 46, 196, 197, 245, 296, 297, 402, 492
PspPI GGNCC 4 cut(s) 124, 195, 223, 295
PspPPI RGGWCCY 3 cut(s) 195, 223, 295
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 3 cut(s) 182, 314, 697
RsaNI GTAC 3 cut(s) 181, 313, 696
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 4 cut(s) 237, 274, 645, 689
Sau3AI GATC 1 cut(s) 25
Sau96I GGNCC 4 cut(s) 124, 195, 223, 295
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 5 cut(s) 30, 192, 193, 241, 317
SfoI GGCGCC 2 cut(s) 402, 492
SfuI TTCGAA 1 cut(s) 659
SinI GGWCC 3 cut(s) 195, 223, 295
SmaI CCCGGG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 3 cut(s) 176, 521, 537
SmoI CTYRAG 3 cut(s) 176, 521, 537
SpeI ACTAGT 1 cut(s) 725
SphI GCATGC 1 cut(s) 587
Sse9I AATT 1 cut(s) 655
SspDI GGCGCC 2 cut(s) 400, 490
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 5 cut(s) 28, 190, 191, 239, 315
TaaI ACNGT 1 cut(s) 108
TaiI ACGT 1 cut(s) 558
TaqI TCGA 4 cut(s) 255, 279, 381, 659
TasI AATT 1 cut(s) 655
TauI GCSGC 2 cut(s) 239, 276
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 91
TseI GCWGC 2 cut(s) 644, 688
TspDTI ATGAA 5 cut(s) 298, 564, 617, 693, 702
TspGWI ACGGA 4 cut(s) 87, 337, 491, 591
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 1 cut(s) 91
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 3 cut(s) 195, 223, 295
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 587
XmaI CCCGGG 1 cut(s) 191
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.