RchiOBHm_Chr2g0119801

4,5-DOPA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005435
Physical Location & Seq
Reverse (-)
32384248 .. 32384871
624 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49248

Sequence Viewer

Length: 348 bp
ATGGGGAAGGCGCTGGCGCCGTTGAGGGAGGAGGGTGTCCTTATTGTGGGGTCGGGGAGCGCGACGCATAATCTAAGGGCGCTGAGGAACACGGACGGGGCGGTGGCGCCGTGGGCGTTGGAGTTTGATACTTGGCTTAAGGAGGCGTTGACTCAAGGAAGATATGAAGACGTGAACCATTATGAGGAGAAAGCTCCGCATGCGAAAACGGCTCATCCCTGGCCTGACCATTTCTACCCTCTTCATGTGGCAATGGGGGCTGCCGGTGCGAATTCGAAGGCCAAGCTTATCCATGAAAGCTGGCAGCTTGGTACTCTTTCTTATGCTTCATACCAGTTTACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

115

Amino Acids

12.67

Weight (kDa)

6.36

Isoelectric Point (pI)

27.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 1 - 113 1.5e-19 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 16, 106
AccII CGCG 1 cut(s) 62
AciI CCGC 2 cut(s) 101, 197
AcsI RAATTY 1 cut(s) 271
AcyI GRCGYC 2 cut(s) 17, 107
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 2 cut(s) 46, 184
AflII CTTAAG 1 cut(s) 137
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 4 cut(s) 194, 286, 300, 307
AluI AGCT 4 cut(s) 194, 286, 300, 307
AoxI GGCC 2 cut(s) 221, 279
ApeKI GCWGC 2 cut(s) 260, 304
ApoI RAATTY 1 cut(s) 271
AspLEI GCGC 5 cut(s) 13, 19, 62, 82, 109
AsuII TTCGAA 1 cut(s) 275
BanI GGYRCC 2 cut(s) 16, 106
BbsI GAAGAC 1 cut(s) 174
BbvCI CCTCAGC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 247, 316
BceAI ACGGC 3 cut(s) 4, 94, 225
BciT130I CCWGG 1 cut(s) 220
BfoI RGCGCY 4 cut(s) 14, 20, 83, 110
BfrI CTTAAG 1 cut(s) 137
BisI GCNGC 2 cut(s) 261, 305
BlsI GCNGC 2 cut(s) 262, 306
Bme1390I CCNGG 1 cut(s) 220
BmgBI CACGTC 1 cut(s) 172
BmiI GGNNCC 2 cut(s) 18, 108
BmrFI CCNGG 1 cut(s) 220
BpiI GAAGAC 1 cut(s) 174
Bpu10I CCTNAGC 1 cut(s) 83
Bpu14I TTCGAA 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 138
BsaHI GRCGYC 2 cut(s) 17, 107
BsaJI CCNNGG 2 cut(s) 110, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 184
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 334, 342
Bse3DI GCAATG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 110, 218
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 46, 184
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 2 cut(s) 334, 342
BseRI GAGGAG 2 cut(s) 44, 200
BseXI GCAGC 2 cut(s) 247, 316
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 223, 281
BshNI GGYRCC 2 cut(s) 16, 106
BsiSI CCGG 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 46, 184
BsnI GGCC 2 cut(s) 223, 281
Bsp119I TTCGAA 1 cut(s) 275
BspACI CCGC 2 cut(s) 101, 197
BspANI GGCC 2 cut(s) 223, 281
BspCNI CTCAG 1 cut(s) 75
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 18, 108
BspT104I TTCGAA 1 cut(s) 275
BspT107I GGYRCC 2 cut(s) 16, 106
BspTI CTTAAG 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 2 cut(s) 334, 342
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 110, 218
BssNI GRCGYC 2 cut(s) 17, 107
Bst2UI CCWGG 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 2 cut(s) 17, 107
BstAFI CTTAAG 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 15, 201, 302
BstDEI CTNAG 2 cut(s) 74, 83
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 4 cut(s) 14, 20, 83, 110
BstHHI GCGC 5 cut(s) 13, 19, 62, 82, 109
BstMWI GCNNNNNNNGC 5 cut(s) 113, 200, 209, 257, 266
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 218
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 2 cut(s) 247, 316
BstV2I GAAGAC 1 cut(s) 174
BsuRI GGCC 2 cut(s) 223, 281
BtgI CCRYGG 1 cut(s) 110
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 3 cut(s) 15, 201, 302
CfoI GCGC 5 cut(s) 13, 19, 62, 82, 109
Cfr10I RCCGGY 1 cut(s) 263
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 312
CviAII CATG 3 cut(s) 200, 245, 293
CviJI RGCY 9 cut(s) 136, 194, 212, 223, 260, 281, 286, 300, 307
CviKI_1 RGCY 9 cut(s) 136, 194, 212, 223, 260, 281, 286, 300, 307
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 2 cut(s) 74, 83
DinI GGCGCC 2 cut(s) 18, 108
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 218
EgeI GGCGCC 2 cut(s) 18, 108
EheI GGCGCC 2 cut(s) 18, 108
FaeI CATG 3 cut(s) 203, 248, 296
FaiI YATR 8 cut(s) 69, 165, 183, 201, 246, 294, 324, 331
FatI CATG 3 cut(s) 199, 244, 292
Fnu4HI GCNGC 2 cut(s) 261, 305
FokI GGATG 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 261, 305
GlaI GCGC 5 cut(s) 12, 18, 61, 81, 108
GluI GCNGC 2 cut(s) 261, 305
HaeII RGCGCY 4 cut(s) 14, 20, 83, 110
HaeIII GGCC 2 cut(s) 223, 281
HapII CCGG 1 cut(s) 264
HgaI GACGC 1 cut(s) 73
HhaI GCGC 5 cut(s) 13, 19, 62, 82, 109
Hin1I GRCGYC 2 cut(s) 17, 107
Hin1II CATG 3 cut(s) 203, 248, 296
Hin6I GCGC 5 cut(s) 11, 17, 60, 80, 107
HinP1I GCGC 5 cut(s) 11, 17, 60, 80, 107
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 1 cut(s) 151
HpaII CCGG 1 cut(s) 264
Hpy166II GTNNAC 3 cut(s) 150, 175, 339
Hpy8I GTNNAC 3 cut(s) 150, 175, 339
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 200, 209, 257, 266
HpyF3I CTNAG 2 cut(s) 74, 83
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 2 cut(s) 17, 107
Hsp92II CATG 3 cut(s) 203, 248, 296
HspAI GCGC 5 cut(s) 11, 17, 60, 80, 107
KasI GGCGCC 2 cut(s) 16, 106
LmnI GCTCC 2 cut(s) 57, 199
LpnPI CCDG 5 cut(s) 205, 232, 237, 277, 286
Lsp1109I GCAGC 2 cut(s) 247, 316
MaeII ACGT 1 cut(s) 171
MboII GAAGA 3 cut(s) 171, 179, 233
MluCI AATT 1 cut(s) 271
Mly113I GGCGCC 2 cut(s) 17, 107
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 7 cut(s) 18, 22, 25, 78, 136, 178, 249
MseI TTAA 1 cut(s) 138
MspCI CTTAAG 1 cut(s) 137
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 220
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 5 cut(s) 113, 200, 209, 257, 266
NarI GGCGCC 2 cut(s) 17, 107
NlaIII CATG 3 cut(s) 203, 248, 296
NlaIV GGNNCC 2 cut(s) 18, 108
NspI RCATGY 1 cut(s) 203
NspV TTCGAA 1 cut(s) 275
PaeI GCATGC 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PkrI GCNGC 2 cut(s) 262, 306
PleI GAGTC 1 cut(s) 145
PluTI GGCGCC 2 cut(s) 20, 110
PpsI GAGTC 1 cut(s) 145
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 2 cut(s) 18, 108
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 2 cut(s) 261, 305
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 220
SetI ASST 5 cut(s) 174, 196, 288, 302, 309
SfoI GGCGCC 2 cut(s) 18, 108
SfuI TTCGAA 1 cut(s) 275
SmlI CTYRAG 2 cut(s) 137, 153
SmoI CTYRAG 2 cut(s) 137, 153
SphI GCATGC 1 cut(s) 203
Sse9I AATT 1 cut(s) 271
SsiI CCGC 2 cut(s) 101, 197
SspDI GGCGCC 2 cut(s) 16, 106
StyD4I CCNGG 1 cut(s) 218
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 275
TasI AATT 1 cut(s) 271
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TseI GCWGC 2 cut(s) 260, 304
TspDTI ATGAA 4 cut(s) 180, 233, 309, 318
TspGWI ACGGA 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 137
XapI RAATTY 1 cut(s) 271
XceI RCATGY 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.