FvH4_1g23880

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005435
Physical Location & Seq
Reverse (-)
15725520 .. 15726311
792 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23880.t1

Sequence Viewer

Length: 792 bp
ATGGACACCTTCTACATCTCCCACGGATCCCCAACCCTAAGCATCGACGAGACCATCCCCGCCGGCCAGTTCCTCCGCTCCTGGAAGCAAAACGGCTTCCCCCAAAAACCTAACTCCATCCTTGTCATCTCCGGCCACTGGGACACCTCCGTCCCCACCGTCAACTCCGTCTCCGGCACTCTCTCCACCATCTACGACTTCTACGGCTTCCCCAAATCCATGTACAAGCTCAAATACCCCGCCCCCGGCGCCCCCGCCCTCGCCTCCCGCGTCAAAGACCTCATCACCGCCGCCGGCATCCCCCGCGTCGACGTCGACCCCGCGCGCGGCCTCGACCACGGCGCGTGGGTCCCACTCATGTTCATGTACCCGGAGGCCGACATTCCGGTCTGCCAGCTGTCGGTGCAGACCGGCCGCGACGGGAGGTACCATTTCGACATGGGGAAGGCGCTGGCGCCGCTGAGGGAGGAGGGGGTGCTGATCGTGGGGTCCGGTAGCGCGACGCATAATCTGAGGGATCTGCGGAACACGGTGGACGGGGCGGTGGCGCCGTGGGCGTTGGAGTTTGATACTTGGCTCAAGGAGGCTTTGACGCAAGGGAAATATGAAGATGTGAATCGTTGGGAGGAGAAGGCTCCCCATGGGAAAAGGGCGCATCCTTGGCCGGATCATTTCTACCCGCTGCATGTGGCCATTGGGGCGGCCGGGGCGGATTCGAAGGCCAAGGTCATCCATGAGAGCTGGCAGCTTGGTACTCTTTCTTATGCTTCATACCAGTTTAGCACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

264

Amino Acids

29.01

Weight (kDa)

6.35

Isoelectric Point (pI)

31.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 3 - 260 3.4e-53 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 386
AatII GACGTC 1 cut(s) 315
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 4 cut(s) 248, 426, 454, 547
AccBSI CCGCTC 1 cut(s) 78
AccI GTMKAC 2 cut(s) 309, 315
AccII CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
AclWI GGATC 4 cut(s) 21, 34, 525, 675
AcoI YGGCCR 6 cut(s) 64, 133, 412, 662, 690, 702
AcyI GRCGYC 4 cut(s) 249, 312, 455, 548
AfaI GTAC 4 cut(s) 224, 368, 428, 754
AfiI CCNNNNNNNGG 4 cut(s) 138, 245, 326, 400
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 4 cut(s) 229, 397, 741, 748
AluI AGCT 4 cut(s) 229, 397, 741, 748
Alw26I GTCTC 2 cut(s) 44, 175
AlwI GGATC 4 cut(s) 21, 34, 525, 675
AoxI GGCC 9 cut(s) 64, 133, 328, 375, 412, 662, 690, 702, 720
ApeKI GCWGC 2 cut(s) 682, 745
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 9 cut(s) 251, 325, 327, 344, 451, 457, 500, 550, 655
AspS9I GGNCC 2 cut(s) 349, 489
AsuC2I CCSGG 3 cut(s) 246, 371, 706
AsuHPI GGTGA 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 716
AvaII GGWCC 2 cut(s) 349, 489
BalI TGGCCA 1 cut(s) 692
BamHI GGATCC 1 cut(s) 26
BanI GGYRCC 4 cut(s) 248, 426, 454, 547
BbvCI CCTCAGC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 669, 757
BccI CCATC 3 cut(s) 62, 125, 197
BceAI ACGGC 4 cut(s) 109, 220, 355, 535
BciT130I CCWGG 1 cut(s) 82
BcnI CCSGG 3 cut(s) 246, 371, 706
BcoDI GTCTC 2 cut(s) 44, 175
BfoI RGCGCY 4 cut(s) 252, 452, 458, 551
BglI GCCNNNNNGGC 1 cut(s) 698
BisI GCNGC 7 cut(s) 291, 328, 415, 458, 683, 702, 746
BlsI GCNGC 7 cut(s) 292, 329, 416, 459, 684, 703, 747
Bme1390I CCNGG 4 cut(s) 82, 246, 371, 706
Bme18I GGWCC 2 cut(s) 349, 489
BmgT120I GGNCC 2 cut(s) 349, 489
BmiI GGNNCC 9 cut(s) 28, 250, 350, 351, 428, 456, 490, 549, 636
BmrFI CCNGG 4 cut(s) 82, 246, 371, 706
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 3 cut(s) 51, 306, 664
BmuI ACTGGG 1 cut(s) 148
Bpu10I CCTNAGC 2 cut(s) 38, 461
Bpu14I TTCGAA 1 cut(s) 716
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 3 cut(s) 246, 371, 706
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 4 cut(s) 249, 312, 455, 548
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 8 cut(s) 22, 244, 337, 551, 640, 659, 705, 723
BsaWI WCCGGW 2 cut(s) 385, 491
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 245, 326, 400
Bse118I RCCGGY 3 cut(s) 62, 293, 410
Bse1I ACTGG 3 cut(s) 67, 143, 775
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 8 cut(s) 22, 244, 337, 551, 640, 659, 705, 723
BseGI GGATG 5 cut(s) 54, 117, 297, 655, 729
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 4 cut(s) 138, 245, 326, 400
BseMII CTCAG 2 cut(s) 452, 503
BseNI ACTGG 3 cut(s) 67, 143, 775
BsePI GCGCGC 1 cut(s) 323
BseRI GAGGAG 2 cut(s) 482, 641
BseX3I CGGCCG 2 cut(s) 412, 702
BseXI GCAGC 2 cut(s) 669, 757
BsgI GTGCAG 1 cut(s) 425
Bsh1236I CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
Bsh1285I CGRYCG 2 cut(s) 415, 705
BshFI GGCC 9 cut(s) 66, 135, 330, 377, 414, 664, 692, 704, 722
BshNI GGYRCC 4 cut(s) 248, 426, 454, 547
BsiEI CGRYCG 2 cut(s) 415, 705
BslFI GGGAC 3 cut(s) 137, 155, 335
BslI CCNNNNNNNGG 4 cut(s) 138, 245, 326, 400
BsmAI GTCTC 2 cut(s) 44, 175
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 3 cut(s) 137, 155, 335
BsnI GGCC 9 cut(s) 66, 135, 330, 377, 414, 664, 692, 704, 722
Bso31I GGTCTC 1 cut(s) 44
Bsp119I TTCGAA 1 cut(s) 716
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 4 cut(s) 26, 480, 517, 667
Bsp19I CCATGG 1 cut(s) 640
BspANI GGCC 9 cut(s) 66, 135, 330, 377, 414, 664, 692, 704, 722
BspCNI CTCAG 2 cut(s) 453, 504
BspFNI CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
BspLI GGNNCC 9 cut(s) 28, 250, 350, 351, 428, 456, 490, 549, 636
BspPI GGATC 4 cut(s) 21, 34, 525, 675
BspT104I TTCGAA 1 cut(s) 716
BspT107I GGYRCC 4 cut(s) 248, 426, 454, 547
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 78
BsrFI RCCGGY 3 cut(s) 62, 293, 410
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 3 cut(s) 67, 143, 775
BssAI RCCGGY 3 cut(s) 62, 293, 410
BssECI CCNNGG 8 cut(s) 22, 244, 337, 551, 640, 659, 705, 723
BssHII GCGCGC 1 cut(s) 323
BssMI GATC 4 cut(s) 26, 480, 517, 667
BssNI GRCGYC 4 cut(s) 249, 312, 455, 548
BssT1I CCWWGG 3 cut(s) 640, 659, 723
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 2 cut(s) 160, 532
BstACI GRCGYC 4 cut(s) 249, 312, 455, 548
BstAUI TGTACA 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 6 cut(s) 64, 295, 325, 395, 453, 743
BstDEI CTNAG 3 cut(s) 38, 461, 512
BstDSI CCRYGG 4 cut(s) 22, 337, 551, 640
BstF5I GGATG 5 cut(s) 54, 117, 297, 655, 729
BstFNI CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
BstH2I RGCGCY 4 cut(s) 252, 452, 458, 551
BstHHI GCGC 9 cut(s) 251, 325, 327, 344, 451, 457, 500, 550, 655
BstKTI GATC 4 cut(s) 29, 483, 520, 670
BstMAI GTCTC 2 cut(s) 44, 175
BstMBI GATC 4 cut(s) 26, 480, 517, 667
BstMCI CGRYCG 2 cut(s) 415, 705
BstMWI GCNNNNNNNGC 8 cut(s) 248, 303, 403, 457, 554, 661, 698, 707
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 80, 244, 369, 704
BstUI CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
BstV1I GCAGC 2 cut(s) 669, 757
BstX2I RGATCY 2 cut(s) 26, 517
BstYI RGATCY 2 cut(s) 26, 517
BstZI CGGCCG 2 cut(s) 412, 702
BsuRI GGCC 9 cut(s) 66, 135, 330, 377, 414, 664, 692, 704, 722
BtgI CCRYGG 4 cut(s) 22, 337, 551, 640
BtsCI GGATG 5 cut(s) 54, 117, 297, 655, 729
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 6 cut(s) 64, 295, 325, 395, 453, 743
CfoI GCGC 9 cut(s) 251, 325, 327, 344, 451, 457, 500, 550, 655
Cfr10I RCCGGY 3 cut(s) 62, 293, 410
Cfr13I GGNCC 2 cut(s) 349, 489
CseI GACGC 4 cut(s) 259, 295, 511, 601
Csp6I GTAC 4 cut(s) 223, 367, 427, 753
CviAII CATG 7 cut(s) 220, 358, 364, 439, 641, 686, 734
CviQI GTAC 4 cut(s) 223, 367, 427, 753
DdeI CTNAG 3 cut(s) 38, 461, 512
DinI GGCGCC 3 cut(s) 250, 456, 549
DpnI GATC 4 cut(s) 28, 482, 519, 669
DpnII GATC 4 cut(s) 26, 480, 517, 667
DrdI GACNNNNNNGTC 2 cut(s) 149, 386
DseDI GACNNNNNNGTC 2 cut(s) 149, 386
EaeI YGGCCR 6 cut(s) 64, 133, 412, 662, 690, 702
EagI CGGCCG 2 cut(s) 412, 702
EciI GGCGGA 1 cut(s) 725
EclXI CGGCCG 2 cut(s) 412, 702
Eco130I CCWWGG 3 cut(s) 640, 659, 723
Eco31I GGTCTC 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 349, 489
Eco52I CGGCCG 2 cut(s) 412, 702
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 3 cut(s) 640, 659, 723
EgeI GGCGCC 3 cut(s) 250, 456, 549
EheI GGCGCC 3 cut(s) 250, 456, 549
ErhI CCWWGG 3 cut(s) 640, 659, 723
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 7 cut(s) 223, 361, 367, 442, 644, 689, 737
FaqI GGGAC 3 cut(s) 137, 155, 335
FatI CATG 7 cut(s) 219, 357, 363, 438, 640, 685, 733
FauI CCCGC 7 cut(s) 67, 247, 262, 275, 311, 328, 687
FblI GTMKAC 2 cut(s) 309, 315
Fnu4HI GCNGC 7 cut(s) 291, 328, 415, 458, 683, 702, 746
FokI GGATG 5 cut(s) 41, 104, 284, 642, 716
Fsp4HI GCNGC 7 cut(s) 291, 328, 415, 458, 683, 702, 746
GlaI GCGC 9 cut(s) 250, 324, 326, 343, 450, 456, 499, 549, 654
GluI GCNGC 7 cut(s) 291, 328, 415, 458, 683, 702, 746
HaeII RGCGCY 4 cut(s) 252, 452, 458, 551
HaeIII GGCC 9 cut(s) 66, 135, 330, 377, 414, 664, 692, 704, 722
HgaI GACGC 4 cut(s) 259, 295, 511, 601
HhaI GCGC 9 cut(s) 251, 325, 327, 344, 451, 457, 500, 550, 655
Hin1I GRCGYC 4 cut(s) 249, 312, 455, 548
Hin1II CATG 7 cut(s) 223, 361, 367, 442, 644, 689, 737
Hin6I GCGC 9 cut(s) 249, 323, 325, 342, 449, 455, 498, 548, 653
HinP1I GCGC 9 cut(s) 249, 323, 325, 342, 449, 455, 498, 548, 653
HincII GTYRAC 3 cut(s) 163, 310, 316
HindII GTYRAC 3 cut(s) 163, 310, 316
HinfI GANTC 2 cut(s) 616, 713
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 4 cut(s) 163, 310, 316, 535
Hpy188I TCNGA 1 cut(s) 513
Hpy8I GTNNAC 4 cut(s) 163, 310, 316, 535
Hpy99I CGWCG 6 cut(s) 50, 311, 314, 317, 422, 505
HpyAV CCTTC 4 cut(s) 19, 439, 625, 712
HpyCH4III ACNGT 2 cut(s) 160, 532
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 406, 685
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 303, 403, 457, 554, 661, 698, 707
HpyF3I CTNAG 3 cut(s) 38, 461, 512
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 4 cut(s) 249, 312, 455, 548
Hsp92II CATG 7 cut(s) 223, 361, 367, 442, 644, 689, 737
HspAI GCGC 9 cut(s) 249, 323, 325, 342, 449, 455, 498, 548, 653
KasI GGCGCC 3 cut(s) 248, 454, 547
KflI GGGWCCC 1 cut(s) 349
KpnI GGTACC 1 cut(s) 430
KroI GCCGGC 2 cut(s) 62, 293
KroNI GCCGGC 2 cut(s) 64, 295
Kzo9I GATC 4 cut(s) 26, 480, 517, 667
LmnI GCTCC 2 cut(s) 83, 640
Lsp1109I GCAGC 2 cut(s) 669, 757
LweI GCATC 3 cut(s) 51, 306, 664
MaeII ACGT 1 cut(s) 312
MalI GATC 4 cut(s) 28, 482, 519, 669
MauBI CGCGCGCG 1 cut(s) 323
MbiI CCGCTC 1 cut(s) 78
MboI GATC 4 cut(s) 26, 480, 517, 667
MboII GAAGA 1 cut(s) 620
MfeI CAATTG 1 cut(s) 787
MflI RGATCY 2 cut(s) 26, 517
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 1 cut(s) 787
MluNI TGGCCA 1 cut(s) 692
Mly113I GGCGCC 3 cut(s) 249, 455, 548
MmeI TCCRAC 1 cut(s) 540
Mox20I TGGCCA 1 cut(s) 692
MroNI GCCGGC 2 cut(s) 62, 293
MscI TGGCCA 1 cut(s) 692
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 692
MspA1I CMGCKG 3 cut(s) 397, 460, 682
MspR9I CCNGG 4 cut(s) 82, 246, 371, 706
MunI CAATTG 1 cut(s) 787
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 8 cut(s) 270, 306, 323, 325, 327, 344, 417, 500
MwoI GCNNNNNNNGC 8 cut(s) 248, 303, 403, 457, 554, 661, 698, 707
NaeI GCCGGC 2 cut(s) 64, 295
NarI GGCGCC 3 cut(s) 249, 455, 548
NciI CCSGG 3 cut(s) 246, 371, 706
NcoI CCATGG 1 cut(s) 640
NdeII GATC 4 cut(s) 26, 480, 517, 667
NgoMIV GCCGGC 2 cut(s) 62, 293
NlaIII CATG 7 cut(s) 223, 361, 367, 442, 644, 689, 737
NlaIV GGNNCC 9 cut(s) 28, 250, 350, 351, 428, 456, 490, 549, 636
NspI RCATGY 1 cut(s) 689
NspV TTCGAA 1 cut(s) 716
PauI GCGCGC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 2 cut(s) 64, 295
PfeI GAWTC 2 cut(s) 616, 713
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 7 cut(s) 292, 329, 416, 459, 684, 703, 747
PluTI GGCGCC 3 cut(s) 252, 458, 551
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 9 cut(s) 28, 250, 350, 351, 428, 456, 490, 549, 636
PspPI GGNCC 2 cut(s) 349, 489
PspPPI RGGWCCY 1 cut(s) 349
PsuI RGATCY 2 cut(s) 26, 517
PteI GCGCGC 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 4 cut(s) 224, 368, 428, 754
RsaNI GTAC 4 cut(s) 223, 367, 427, 753
RseI CAYNNNNRTG 1 cut(s) 362
SalI GTCGAC 2 cut(s) 308, 314
SatI GCNGC 7 cut(s) 291, 328, 415, 458, 683, 702, 746
Sau3AI GATC 4 cut(s) 26, 480, 517, 667
Sau96I GGNCC 2 cut(s) 349, 489
ScrFI CCNGG 4 cut(s) 82, 246, 371, 706
SfaNI GCATC 3 cut(s) 51, 306, 664
SfoI GGCGCC 3 cut(s) 250, 456, 549
SfuI TTCGAA 1 cut(s) 716
SgrDI CGTCGACG 1 cut(s) 308
SinI GGWCC 2 cut(s) 349, 489
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 1 cut(s) 787
SspDI GGCGCC 3 cut(s) 248, 454, 547
StyD4I CCNGG 4 cut(s) 80, 244, 369, 704
StyI CCWWGG 3 cut(s) 640, 659, 723
TaaI ACNGT 2 cut(s) 160, 532
TaiI ACGT 1 cut(s) 315
TaqI TCGA 6 cut(s) 45, 309, 315, 333, 435, 716
TasI AATT 1 cut(s) 787
TatI WGTACW 1 cut(s) 222
TauI GCSGC 5 cut(s) 293, 330, 417, 460, 704
TfiI GAWTC 2 cut(s) 616, 713
TscAI CASTG 1 cut(s) 143
TseI GCWGC 2 cut(s) 682, 745
TspDTI ATGAA 3 cut(s) 352, 621, 759
TspGWI ACGGA 3 cut(s) 39, 139, 157
TspRI CASTG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 349, 489
XceI RCATGY 1 cut(s) 689
XmiI GTMKAC 2 cut(s) 309, 315
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.