Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0005435

Physical Location & Seq

Genomic Coordinates Forward (+)

21697108 .. 21699144

Gene Structure 2037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0223900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGGCCACCGCTCTCATCAACATATCATCAGTACCTATCAATATCCTCATTCTGCTCTTCATTCTGCCTTCAGCTTGCTCAACTAATTTCAAAATAGCACGATTTGATGCAACTGCAGCCAACATAGTCTATGAGGGAGATGCAGTCCCTTCTGTGGGAGTAATTGAACTCATTGACAAAGTCAATTATGTTTGCCGCGTTGGCAGGGCCACCTATGCTGAGAGAGTTCCATTGTGGGACTCTCATGCTGGGAAGGCTGCTGACTTCACAACCCATTTCTCATTCATCGTTGACACACAAGGTGCAGCTACTTATGGCAGTGGATTCGGATTCTTTCTCGCTCCTTTTGGATTTCAAATCCCGCTAAATTCAGCTGGTGGATTTCTTGGTCTATTTAACACCACAACCAGTGACTTTGCTGGGAACCATATTGTTCTAGTTGAGTTTGATTCCTACGTAAACCCTGACTGGGATCCTGCATATCAGCATGTGGGGATCAACAACAACTCAATTGCTTCGGTTGTGACGACCCCTTGGAATGTTAGCTTACACGATGGAGACACTACTGATGTATGGATTTCCTACAATGCTACAACAACAAGTCTAATTGTTCAGTGGAGCTACCAAAATACCCCTGCTTCAGCAGAGGAGACAACTAGCATCTCTTACCAAATTGATCTTATGGAAGTCCTGCCTGAGTGGATCGTAATTGGATTTTCTGCTGGTACTAGTCAATATATGGAGCGAAATAAGATTGTCTCATGGGAATTCAACTCAACTCTGGATAGAAAATATCCTGGTTCAAGTACCGGAAACCATGCAAAGAAGATGATGATGATAATAGTAATTACACTACTAGCAGTTTCAGTGGTTTCTCTGATAGTTGGGATGATTACAGCAAAAACTCTGGGGTTGTGTCGTTCCCGGTTGAAGAAAACAGAGAAAAAAGAAGAGGAGATAGAAGTACCTGCAGTAATAGCCACTGGAAACTTAGCACCAATCATTGAAGAAAATAGTGAAGGAGAAGCAGGACAGGGTTGGTTCACTTGTTCCAACATTGTTACAGCTACTAACAATTTCTCCAATGACAGGAAGTTGGGTGAAGGAGGGTCTGGTACTGTATACAGGGGTGAGTTTGCTGACATAAATATGACAGTAGCAGTGAAGAAAATTTTAAACAAGTCTGGTAAGGGAAGAAAGGAGTACATAACGGAGGTGAATCTAATTAGTAGCTTGAGGCACCCAAATCTGGTGCAACTCAAAGGATGGTGCCATGATGGAGGTCAGACTACATTCCTTCTTGTTTACGAGTTCATGCCAGAGGGTAGCCTCGATTCTCACCTCTTTGGGGCGAGGCCTGCTCTAACCTGGAGTGTGAGATACAAGATATATAAGGGACTGGCCTCAGCATTGCTTTATCTCCACGATGCATATCTTCATGAAGGGTGGAAGCAATGTGTGGTGCATCGAGATATTAAGTCGAGTAATATCCTGTTGGACTCTAGCTTTAATGTCAAGTTAGGCGACTTTGGGTTAGCTCGGATAATGTATCATGAGCAAGATCCCAGGACAACTGAGTTGGCTGGAACATTTGGCTATATGGATCCAGAATACATAATCACTGGCAAGCCTAGTAGAGAGTCAGATGTTTATAGCTTTGGGGTGGTGGCCTTAGAAATTGCTACTGGAAAAAAGTCGGTGTATCATATGGGAGACAATTCCAGAAAGGGACTCGTACAGTGGGTTTGGGATCTCTATGGACAAGGAGATCTTTTCTCTGCAGTGGATCAGAGGCTGCACTGGAACTATGATGAGAAACAAGTTGAGTGTTTGATGATTGTAGGACTGTGGTGCGCTCATCCTGATAAAAATCAAAGGCCCTTAATAAGGCAAGCAATTCAATATCTCAACTTCGATGTAGAATTGCCTAGACCTCCAATGACGATGCCTTGTCCGGTGTATCAGGTACCTATACCACTGCTCTCAATCATCTCCGGTGCATCGTCAAGTACTACAAGCCTTCAAGAGGGTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00965 map00965

Pfam Domains

Protein Families

LigB

Protein Analysis

678

Amino Acids

75.03

Weight (kDa)

5.63

Isoelectric Point (pI)

32.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	28 - 263	1.5e-65	Legume lectin domain
Pkinase	PF00069	361 - 634	2.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	363 - 572	2.1e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15093
fragaria_vesca	FvH4_1g23880
malus_domestica	MD00G1165300.v1.1 MD15G1340700.v1.1
prunus_persica	Prupe.6G161400_v2.0.a1
pyrus_communis	pycom15g30030
rosa_chinensis	RchiOBHm_Chr2g0119801 RchiOBHm_Chr2g0119841 RchiOBHm_Chr6g0259111 RchiOBHm_Chr6g0259131
rosa_laevigata	RLG00000014556 RLG00000014558 RLG00000018494 RLG00000018495
rosa_multiflora	Rmu_sc0001501.1_g000043
rosa_roxburghii	Rroxscaffold_2G00124060
rosa_rugosa	Rorug02G0223900 Rorug02G0224100
rosa_samantha	Rh2AG281600 Rh2AG281900 Rh2BG293200 Rh2BG293300 Rh2DG288000 Rh2DG288200 Rh2DG307300 Rh6AG102000 Rh6BG094200 Rh6CG091400
rosa_wichuraiana	Rw2G022510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	976
Acc65I	GGTACC	1 cut(s)	1966
AccB1I	GGYRCC	3 cut(s)	1239, 1269, 1966
AccBSI	CCGCTC	1 cut(s)	11
AccI	GTMKAC	1 cut(s)	1122
AccII	CGCG	1 cut(s)	198
AciI	CCGC	3 cut(s)	9, 196, 362
AclWI	GGATC	9 cut(s)	467, 480, 503, 710, 1556, 1598, 1611, 1758, 1794
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	367, 767, 1170
AcuI	CTGAAG	2 cut(s)	54, 624
AdeI	CACNNNGTG	1 cut(s)	302
AfaI	GTAC	9 cut(s)	33, 727, 808, 966, 1117, 1205, 1737, 1968, 2011
AfiI	CCNNNNNNNGG	8 cut(s)	154, 155, 469, 1089, 1249, 1348, 1887, 2026
AgsI	TTSAA	9 cut(s)	91, 167, 356, 772, 804, 931, 1007, 1901, 2024
AhlI	ACTAGT	1 cut(s)	728
AjnI	CCWGG	3 cut(s)	796, 1367, 1565
Alw26I	GTCTC	4 cut(s)	552, 644, 763, 1707
AlwI	GGATC	9 cut(s)	467, 480, 503, 710, 1556, 1598, 1611, 1758, 1794
AlwNI	CAGNNNCTG	1 cut(s)	1795
AoxI	GGCC	6 cut(s)	3, 207, 1355, 1401, 1668, 1877
ApeKI	GCWGC	4 cut(s)	116, 257, 305, 1795
ApoI	RAATTY	3 cut(s)	367, 767, 1170
Asp718I	GGTACC	1 cut(s)	1966
AspLEI	GCGC	1 cut(s)	1856
AspS9I	GGNCC	2 cut(s)	207, 1878
AsuC2I	CCSGG	1 cut(s)	925
AsuHPI	GGTGA	4 cut(s)	1112, 1142, 1228, 1331
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	472, 1603
BanI	GGYRCC	3 cut(s)	1239, 1269, 1966
BbvCI	CCTCAGC	1 cut(s)	1405
BbvI	GCAGC	4 cut(s)	128, 244, 317, 1782
BccI	CCATC	3 cut(s)	548, 1260, 1271
BcgI	CGANNNNNNTGC	2 cut(s)	307, 341
BciT130I	CCWGG	3 cut(s)	798, 1369, 1567
BcnI	CCSGG	1 cut(s)	925
BcoDI	GTCTC	4 cut(s)	552, 644, 763, 1707
BcuI	ACTAGT	1 cut(s)	728
BfaI	CTAG	7 cut(s)	437, 657, 729, 857, 1503, 1632, 1929
BfmI	CTRYAG	3 cut(s)	114, 969, 1779
BfuAI	ACCTGC	1 cut(s)	976
BglI	GCCNNNNNGGC	1 cut(s)	201
BglII	AGATCT	1 cut(s)	1768
BisI	GCNGC	5 cut(s)	117, 196, 258, 306, 1796
BlsI	GCNGC	5 cut(s)	118, 197, 259, 307, 1797
BmcAI	AGTACT	1 cut(s)	2011
Bme1390I	CCNGG	4 cut(s)	798, 925, 1369, 1567
BmgT120I	GGNCC	2 cut(s)	207, 1878
BmiI	GGNNCC	6 cut(s)	425, 474, 1241, 1271, 1605, 1968
BmrFI	CCNGG	4 cut(s)	798, 925, 1369, 1567
BmrI	ACTGGG	1 cut(s)	478
BmsI	GCATC	7 cut(s)	97, 130, 669, 1417, 1474, 1935, 2009
BmuI	ACTGGG	1 cut(s)	478
BplI	GAGNNNNNCTC	2 cut(s)	1345, 1377
BpmI	CTGGAG	1 cut(s)	1390
Bpu10I	CCTNAGC	1 cut(s)	1405
BpuEI	CTTGAG	1 cut(s)	1255
BpuMI	CCSGG	1 cut(s)	925
BsaAI	YACGTR	1 cut(s)	457
BsaBI	GATNNNNATC	2 cut(s)	1431, 1869
BsaJI	CCNNGG	2 cut(s)	533, 1565
BsaWI	WCCGGW	3 cut(s)	809, 1954, 1994
BsaXI	ACNNNNNCTCC	4 cut(s)	129, 159, 1064, 1094
Bsc4I	CCNNNNNNNGG	8 cut(s)	154, 155, 469, 1089, 1249, 1348, 1887, 2026
Bse1I	ACTGG	7 cut(s)	408, 473, 988, 1404, 1627, 1690, 1805
Bse3DI	GCAATG	2 cut(s)	1409, 1460
Bse8I	GATNNNNATC	2 cut(s)	1431, 1869
BseBI	CCWGG	3 cut(s)	798, 1369, 1567
BseDI	CCNNGG	2 cut(s)	533, 1565
BseGI	GGATG	3 cut(s)	894, 1271, 1858
BseJI	GATNNNNATC	2 cut(s)	1431, 1869
BseLI	CCNNNNNNNGG	8 cut(s)	154, 155, 469, 1089, 1249, 1348, 1887, 2026
BseMI	GCAATG	2 cut(s)	1409, 1460
BseMII	CTCAG	4 cut(s)	210, 687, 1419, 1566
BseNI	ACTGG	7 cut(s)	408, 473, 988, 1404, 1627, 1690, 1805
BseRI	GAGGAG	2 cut(s)	662, 968
BseXI	GCAGC	4 cut(s)	128, 244, 317, 1782
BseYI	CCCAGC	2 cut(s)	248, 419
BsgI	GTGCAG	2 cut(s)	324, 1781
Bsh1236I	CGCG	1 cut(s)	198
BshFI	GGCC	6 cut(s)	5, 209, 1357, 1403, 1670, 1879
BshNI	GGYRCC	3 cut(s)	1239, 1269, 1966
BsiSI	CCGG	4 cut(s)	810, 925, 1955, 1995
BslFI	GGGAC	4 cut(s)	131, 251, 1410, 1743
BslI	CCNNNNNNNGG	8 cut(s)	154, 155, 469, 1089, 1249, 1348, 1887, 2026
BsmAI	GTCTC	4 cut(s)	552, 644, 763, 1707
BsmFI	GGGAC	4 cut(s)	131, 251, 1410, 1743
BsnI	GGCC	6 cut(s)	5, 209, 1357, 1403, 1670, 1879
Bsp143I	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
BspACI	CCGC	3 cut(s)	9, 196, 362
BspANI	GGCC	6 cut(s)	5, 209, 1357, 1403, 1670, 1879
BspCNI	CTCAG	4 cut(s)	211, 688, 1418, 1567
BspFNI	CGCG	1 cut(s)	198
BspHI	TCATGA	2 cut(s)	1438, 1552
BspLI	GGNNCC	6 cut(s)	425, 474, 1241, 1271, 1605, 1968
BspMAI	CTGCAG	3 cut(s)	118, 973, 1783
BspMI	ACCTGC	1 cut(s)	976
BspPI	GGATC	9 cut(s)	467, 480, 503, 710, 1556, 1598, 1611, 1758, 1794
BspQI	GCTCTTC	1 cut(s)	62
BspT107I	GGYRCC	3 cut(s)	1239, 1269, 1966
BsrBI	CCGCTC	1 cut(s)	11
BsrDI	GCAATG	2 cut(s)	1409, 1460
BsrI	ACTGG	7 cut(s)	408, 473, 988, 1404, 1627, 1690, 1805
BssECI	CCNNGG	2 cut(s)	533, 1565
BssMI	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
BssNAI	GTATAC	1 cut(s)	1123
BssT1I	CCWWGG	1 cut(s)	533
Bst1107I	GTATAC	1 cut(s)	1123
Bst2UI	CCWGG	3 cut(s)	798, 1369, 1567
Bst4CI	ACNGT	4 cut(s)	1120, 1156, 1740, 1848
Bst6I	CTCTTC	2 cut(s)	62, 945
BstBAI	YACGTR	1 cut(s)	457
BstC8I	GCNNGC	4 cut(s)	76, 1359, 1628, 1893
BstDEI	CTNAG	6 cut(s)	219, 696, 991, 1405, 1575, 1672
BstENI	CCTNNNNNAGG	2 cut(s)	1885, 2024
BstF5I	GGATG	3 cut(s)	894, 1271, 1858
BstFNI	CGCG	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	1856
BstKTI	GATC	9 cut(s)	475, 498, 679, 705, 1564, 1606, 1753, 1771, 1789
BstMAI	GTCTC	4 cut(s)	552, 644, 763, 1707
BstMBI	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
BstMWI	GCNNNNNNNGC	6 cut(s)	116, 201, 215, 254, 977, 1358
BstNI	CCWGG	3 cut(s)	798, 1369, 1567
BstNSI	RCATGY	1 cut(s)	491
BstSCI	CCNGG	4 cut(s)	796, 923, 1367, 1565
BstSFI	CTRYAG	3 cut(s)	114, 969, 1779
BstSNI	TACGTA	1 cut(s)	457
BstUI	CGCG	1 cut(s)	198
BstV1I	GCAGC	4 cut(s)	128, 244, 317, 1782
BstX2I	RGATCY	5 cut(s)	472, 1561, 1603, 1750, 1768
BstYI	RGATCY	5 cut(s)	472, 1561, 1603, 1750, 1768
BstZ17I	GTATAC	1 cut(s)	1123
BsuRI	GGCC	6 cut(s)	5, 209, 1357, 1403, 1670, 1879
BtsCI	GGATG	3 cut(s)	894, 1271, 1858
BtsI	GCAGTG	4 cut(s)	325, 1167, 1788, 1976
BveI	ACCTGC	1 cut(s)	976
Cac8I	GCNNGC	4 cut(s)	76, 1359, 1628, 1893
CaiI	CAGNNNCTG	1 cut(s)	1795
CciI	TCATGA	2 cut(s)	1438, 1552
CfoI	GCGC	1 cut(s)	1856
Cfr13I	GGNCC	2 cut(s)	207, 1878
Csp6I	GTAC	9 cut(s)	32, 726, 807, 965, 1116, 1204, 1736, 1967, 2010
CviAII	CATG	8 cut(s)	245, 488, 762, 818, 1274, 1315, 1439, 1553
CviQI	GTAC	9 cut(s)	32, 726, 807, 965, 1116, 1204, 1736, 1967, 2010
DdeI	CTNAG	6 cut(s)	219, 696, 991, 1405, 1575, 1672
DpnI	GATC	9 cut(s)	474, 497, 678, 704, 1563, 1605, 1752, 1770, 1788
DpnII	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
DraI	TTTAAA	1 cut(s)	1176
DraIII	CACNNNGTG	1 cut(s)	302
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	62, 945
EarI	CTCTTC	2 cut(s)	62, 945
Eco105I	TACGTA	1 cut(s)	457
Eco130I	CCWWGG	1 cut(s)	533
Eco147I	AGGCCT	1 cut(s)	1357
Eco57I	CTGAAG	2 cut(s)	54, 624
EcoNI	CCTNNNNNAGG	2 cut(s)	1885, 2024
EcoO109I	RGGNCCY	1 cut(s)	1878
EcoRI	GAATTC	1 cut(s)	767
EcoRII	CCWGG	3 cut(s)	796, 1367, 1565
EcoT14I	CCWWGG	1 cut(s)	533
EcoT22I	ATGCAT	1 cut(s)	1432
ErhI	CCWWGG	1 cut(s)	533
FaeI	CATG	8 cut(s)	248, 491, 765, 821, 1277, 1318, 1442, 1556
FalI	AAGNNNNNCTT	2 cut(s)	1755, 1787
FaqI	GGGAC	4 cut(s)	131, 251, 1410, 1743
FatI	CATG	8 cut(s)	244, 487, 761, 817, 1273, 1314, 1438, 1552
FauI	CCCGC	1 cut(s)	369
FauNDI	CATATG	1 cut(s)	1707
FblI	GTMKAC	1 cut(s)	1122
Fnu4HI	GCNGC	5 cut(s)	117, 196, 258, 306, 1796
FokI	GGATG	3 cut(s)	901, 1278, 1845
Fsp4HI	GCNGC	5 cut(s)	117, 196, 258, 306, 1796
FspBI	CTAG	7 cut(s)	437, 657, 729, 857, 1503, 1632, 1929
GlaI	GCGC	1 cut(s)	1855
GluI	GCNGC	5 cut(s)	117, 196, 258, 306, 1796
GsaI	CCCAGC	2 cut(s)	252, 423
GsuI	CTGGAG	1 cut(s)	1390
HaeIII	GGCC	6 cut(s)	5, 209, 1357, 1403, 1670, 1879
HapII	CCGG	4 cut(s)	810, 925, 1955, 1995
HhaI	GCGC	1 cut(s)	1856
Hin1II	CATG	8 cut(s)	248, 491, 765, 821, 1277, 1318, 1442, 1556
Hin6I	GCGC	1 cut(s)	1854
HinP1I	GCGC	1 cut(s)	1854
HincII	GTYRAC	1 cut(s)	292
HindII	GTYRAC	1 cut(s)	292
HinfI	GANTC	9 cut(s)	239, 324, 330, 449, 1219, 1334, 1499, 1640, 1731
HpaII	CCGG	4 cut(s)	810, 925, 1955, 1995
HphI	GGTGA	4 cut(s)	1112, 1142, 1228, 1331
Hpy166II	GTNNAC	5 cut(s)	292, 460, 1044, 1123, 1306
Hpy188I	TCNGA	6 cut(s)	329, 879, 1287, 1542, 1645, 1791
Hpy188III	TCNNGA	8 cut(s)	782, 1439, 1469, 1553, 1607, 1722, 1862, 2024
Hpy8I	GTNNAC	5 cut(s)	292, 460, 1044, 1123, 1306
HpyAV	CCTTC	8 cut(s)	78, 159, 247, 1013, 1097, 1307, 1436, 2030
HpyCH4III	ACNGT	4 cut(s)	1120, 1156, 1740, 1848
HpyCH4IV	ACGT	1 cut(s)	456
HpyF10VI	GCNNNNNNNGC	6 cut(s)	116, 201, 215, 254, 977, 1358
HpyF3I	CTNAG	6 cut(s)	219, 696, 991, 1405, 1575, 1672
HpySE526I	ACGT	1 cut(s)	456
Hsp92II	CATG	8 cut(s)	248, 491, 765, 821, 1277, 1318, 1442, 1556
HspAI	GCGC	1 cut(s)	1854
KpnI	GGTACC	1 cut(s)	1970
Kzo9I	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
LguI	GCTCTTC	1 cut(s)	62
LmnI	GCTCC	3 cut(s)	346, 618, 742
Lsp1109I	GCAGC	4 cut(s)	128, 244, 317, 1782
LweI	GCATC	7 cut(s)	97, 130, 669, 1417, 1474, 1935, 2009
MaeI	CTAG	7 cut(s)	437, 657, 729, 857, 1503, 1632, 1929
MaeII	ACGT	1 cut(s)	456
MaeIII	GTNAC	3 cut(s)	410, 523, 1060
MalI	GATC	9 cut(s)	474, 497, 678, 704, 1563, 1605, 1752, 1770, 1788
MbiI	CCGCTC	1 cut(s)	11
MboI	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
MboII	GAAGA	8 cut(s)	49, 838, 943, 962, 1019, 1177, 1206, 1427
MfeI	CAATTG	1 cut(s)	510
MflI	RGATCY	5 cut(s)	472, 1561, 1603, 1750, 1768
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	233, 1493, 1649, 1725
MmeI	TCCRAC	2 cut(s)	1077, 1476
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	1432
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	396, 1175, 1476, 1509, 1883
MslI	CAYNNNNRTG	1 cut(s)	1148
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	374
MspI	CCGG	4 cut(s)	810, 925, 1955, 1995
MspR9I	CCNGG	4 cut(s)	798, 925, 1369, 1567
MunI	CAATTG	1 cut(s)	510
MvaI	CCWGG	3 cut(s)	798, 1369, 1567
MvnI	CGCG	1 cut(s)	198
MwoI	GCNNNNNNNGC	6 cut(s)	116, 201, 215, 254, 977, 1358
NciI	CCSGG	1 cut(s)	925
NdeI	CATATG	1 cut(s)	1707
NdeII	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
NlaIII	CATG	8 cut(s)	248, 491, 765, 821, 1277, 1318, 1442, 1556
NlaIV	GGNNCC	6 cut(s)	425, 474, 1241, 1271, 1605, 1968
NmuCI	GTSAC	2 cut(s)	410, 523
NsiI	ATGCAT	1 cut(s)	1432
NspI	RCATGY	1 cut(s)	491
PagI	TCATGA	2 cut(s)	1438, 1552
PceI	AGGCCT	1 cut(s)	1357
PciSI	GCTCTTC	1 cut(s)	62
PcsI	WCGNNNNNNNCGW	1 cut(s)	524
PfeI	GAWTC	5 cut(s)	324, 330, 449, 1219, 1334
PflFI	GACNNNGTC	1 cut(s)	179
PkrI	GCNGC	5 cut(s)	118, 197, 259, 307, 1797
PleI	GAGTC	4 cut(s)	233, 1493, 1648, 1725
PpsI	GAGTC	4 cut(s)	233, 1493, 1648, 1725
Ppu21I	YACGTR	1 cut(s)	457
Psp6I	CCWGG	3 cut(s)	796, 1367, 1565
PspFI	CCCAGC	2 cut(s)	248, 419
PspGI	CCWGG	3 cut(s)	796, 1367, 1565
PspN4I	GGNNCC	6 cut(s)	425, 474, 1241, 1271, 1605, 1968
PspPI	GGNCC	2 cut(s)	207, 1878
PstI	CTGCAG	3 cut(s)	118, 973, 1783
PstNI	CAGNNNCTG	1 cut(s)	1795
PsuI	RGATCY	5 cut(s)	472, 1561, 1603, 1750, 1768
PsyI	GACNNNGTC	1 cut(s)	179
PvuII	CAGCTG	1 cut(s)	374
RsaI	GTAC	9 cut(s)	33, 727, 808, 966, 1117, 1205, 1737, 1968, 2011
RsaNI	GTAC	9 cut(s)	32, 726, 807, 965, 1116, 1204, 1736, 1967, 2010
RseI	CAYNNNNRTG	1 cut(s)	1148
SapI	GCTCTTC	1 cut(s)	62
SaqAI	TTAA	5 cut(s)	396, 1175, 1476, 1509, 1883
SatI	GCNGC	5 cut(s)	117, 196, 258, 306, 1796
Sau3AI	GATC	9 cut(s)	472, 495, 676, 702, 1561, 1603, 1750, 1768, 1786
Sau96I	GGNCC	2 cut(s)	207, 1878
ScaI	AGTACT	1 cut(s)	2011
SchI	GAGTC	4 cut(s)	233, 1493, 1649, 1725
ScrFI	CCNGG	4 cut(s)	798, 925, 1369, 1567
SfaNI	GCATC	7 cut(s)	97, 130, 669, 1417, 1474, 1935, 2009
SfcI	CTRYAG	3 cut(s)	114, 969, 1779
SmiMI	CAYNNNNRTG	1 cut(s)	1148
SmlI	CTYRAG	1 cut(s)	1234
SmoI	CTYRAG	1 cut(s)	1234
SnaBI	TACGTA	1 cut(s)	457
SpeI	ACTAGT	1 cut(s)	728
SseBI	AGGCCT	1 cut(s)	1357
SsiI	CCGC	3 cut(s)	9, 196, 362
SspMI	CTAG	7 cut(s)	437, 657, 729, 857, 1503, 1632, 1929
StuI	AGGCCT	1 cut(s)	1357
StyD4I	CCNGG	4 cut(s)	796, 923, 1367, 1565
StyI	CCWWGG	1 cut(s)	533
TaaI	ACNGT	4 cut(s)	1120, 1156, 1740, 1848
TaiI	ACGT	1 cut(s)	459
TaqI	TCGA	4 cut(s)	1332, 1468, 1481, 1914
TatI	WGTACW	2 cut(s)	1203, 2009
TauI	GCSGC	1 cut(s)	198
TfiI	GAWTC	5 cut(s)	324, 330, 449, 1219, 1334
Tru1I	TTAA	5 cut(s)	396, 1175, 1476, 1509, 1883
Tru9I	TTAA	5 cut(s)	396, 1175, 1476, 1509, 1883
TseFI	GTSAC	2 cut(s)	410, 523
TseI	GCWGC	4 cut(s)	116, 257, 305, 1795
Tsp45I	GTSAC	2 cut(s)	410, 523
TspDTI	ATGAA	5 cut(s)	49, 274, 1303, 1427, 1455
TspGWI	ACGGA	1 cut(s)	1226
Tth111I	GACNNNGTC	1 cut(s)	179
XagI	CCTNNNNNAGG	2 cut(s)	1885, 2024
XapI	RAATTY	3 cut(s)	367, 767, 1170
XceI	RCATGY	1 cut(s)	491
XmiI	GTMKAC	1 cut(s)	1122
XspI	CTAG	7 cut(s)	437, 657, 729, 857, 1503, 1632, 1929
ZrmI	AGTACT	1 cut(s)	2011
Zsp2I	ATGCAT	1 cut(s)	1432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.