Rh2AG281600

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005435
Physical Location & Seq
Reverse (-)
34181694 .. 34182488
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG281600.1

Sequence Viewer

Length: 795 bp
ATGGCAGTAATGGACACGTTCTACATCTCCCATGGATCCCCAACCTTAAGTATTGACGAGACCATTCCCGCCGGACAGTTCCTCCGATCCTGGAAACAAAACGGGTTCCCCCAGAAACCCAACTCCATCCTCGTCATCTCCGGCCACTGGGAAACCTCCGTCCCCACCGTCAACTCCATCGCCGGCCCACACGACACCATCTACGACTTCTACGGCTTCCCCAAACCCATGTACAAGCTCAAGTACCCCGCCCCCGGGTCCCCCCGGCTCGCCTCCCGCGTCAAGGACCTCCTCACCGCCGCCGGGTTCCCCCGCGTCGACGTGGACACCAAGCGCGGCCTCGACCACGGCGCGTGGGTTCCGCTCATGTTCATGTACCCGGAGGCGGATGTTCCGGTGTGCCAGCTGTCCGTCCAGACTAACAGGGACGGGAGCTATCACTACGATATGGGGAAGGCGCTGGCGCCGTTGAGGGAGGAGGGTGTCCTTATTGTGGGGTCGGGGAGCGCGACGCATAATCTAAGGGCGCTGAGGAACACGGACGGGGCGGTGGCGCCGTGGGCGTTGGAGTTTGATACTTGGCTTAAGGAGGCGTTGACTCAAGGAAGATATGAAGACGTGAACCATTATGAGGAGAAAGCTCCGCATGCGAAAACGGCTCATCCCTGGCCTGACCATTTCTACCCTCTTCATGTGGCAATGGGGGCTGCCGGTGCGAATTCGAAGGCCAAGCTTATCCATGAAAGCTGGCAGCTTGGTACTCTTTCTTATGCTTCATACCAGTTTACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

264

Amino Acids

29.29

Weight (kDa)

6.45

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 6 - 262 2.4e-53 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 463, 553
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 318
AccII CGCG 5 cut(s) 279, 315, 336, 353, 509
AclWI GGATC 3 cut(s) 30, 43, 81
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 718
AcyI GRCGYC 2 cut(s) 464, 554
AfaI GTAC 4 cut(s) 233, 245, 377, 760
AfiI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 303, 385, 493, 631
AflII CTTAAG 2 cut(s) 46, 584
AflIII ACRYGT 1 cut(s) 15
AjiI CACGTC 2 cut(s) 322, 619
AjnI CCWGG 2 cut(s) 89, 665
AluBI AGCT 7 cut(s) 238, 406, 435, 641, 733, 747, 754
AluI AGCT 7 cut(s) 238, 406, 435, 641, 733, 747, 754
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 30, 43, 81
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 5 cut(s) 142, 184, 337, 668, 726
ApeKI GCWGC 2 cut(s) 707, 751
ApoI RAATTY 1 cut(s) 718
AspLEI GCGC 7 cut(s) 336, 353, 460, 466, 509, 529, 556
AspS9I GGNCC 3 cut(s) 185, 258, 286
AsuC2I CCSGG 5 cut(s) 255, 256, 265, 304, 380
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 2 cut(s) 258, 286
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 2 cut(s) 463, 553
BbsI GAAGAC 1 cut(s) 621
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 2 cut(s) 694, 763
BccI CCATC 3 cut(s) 134, 185, 206
BceAI ACGGC 5 cut(s) 229, 364, 451, 541, 672
BciT130I CCWGG 2 cut(s) 91, 667
BcnI CCSGG 5 cut(s) 255, 256, 265, 304, 380
BcoDI GTCTC 1 cut(s) 53
BfoI RGCGCY 4 cut(s) 461, 467, 530, 557
BfrI CTTAAG 2 cut(s) 46, 584
BisI GCNGC 4 cut(s) 300, 337, 708, 752
BlsI GCNGC 4 cut(s) 301, 338, 709, 753
Bme1390I CCNGG 7 cut(s) 91, 255, 256, 265, 304, 380, 667
Bme18I GGWCC 2 cut(s) 258, 286
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 2 cut(s) 322, 619
BmgT120I GGNCC 3 cut(s) 185, 258, 286
BmiI GGNNCC 8 cut(s) 37, 107, 259, 260, 308, 360, 465, 555
BmrFI CCNGG 7 cut(s) 91, 255, 256, 265, 304, 380, 667
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 621
Bpu10I CCTNAGC 1 cut(s) 530
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 2 cut(s) 224, 585
BpuMI CCSGG 5 cut(s) 255, 256, 265, 304, 380
BsaHI GRCGYC 2 cut(s) 464, 554
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 7 cut(s) 31, 253, 254, 263, 346, 557, 665
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 303, 385, 493, 631
Bse118I RCCGGY 2 cut(s) 182, 710
Bse1I ACTGG 3 cut(s) 152, 781, 789
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 2 cut(s) 91, 667
BseDI CCNNGG 7 cut(s) 31, 253, 254, 263, 346, 557, 665
BseGI GGATG 3 cut(s) 126, 394, 661
BseLI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 303, 385, 493, 631
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 3 cut(s) 152, 781, 789
BseRI GAGGAG 3 cut(s) 281, 491, 647
BseXI GCAGC 2 cut(s) 694, 763
Bsh1236I CGCG 5 cut(s) 279, 315, 336, 353, 509
BshFI GGCC 5 cut(s) 144, 186, 339, 670, 728
BshNI GGYRCC 2 cut(s) 463, 553
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 9 cut(s) 72, 141, 183, 255, 265, 303, 380, 395, 711
BslFI GGGAC 3 cut(s) 146, 244, 440
BslI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 303, 385, 493, 631
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 3 cut(s) 146, 244, 440
BsnI GGCC 5 cut(s) 144, 186, 339, 670, 728
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 722
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 35, 86
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 5 cut(s) 144, 186, 339, 670, 728
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 5 cut(s) 279, 315, 336, 353, 509
BspLI GGNNCC 8 cut(s) 37, 107, 259, 260, 308, 360, 465, 555
BspPI GGATC 3 cut(s) 30, 43, 81
BspT104I TTCGAA 1 cut(s) 722
BspT107I GGYRCC 2 cut(s) 463, 553
BspTI CTTAAG 2 cut(s) 46, 584
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 2 cut(s) 182, 710
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 152, 781, 789
BssAI RCCGGY 2 cut(s) 182, 710
BssECI CCNNGG 7 cut(s) 31, 253, 254, 263, 346, 557, 665
BssMI GATC 2 cut(s) 35, 86
BssNI GRCGYC 2 cut(s) 464, 554
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 91, 667
Bst4CI ACNGT 2 cut(s) 78, 169
Bst6I CTCTTC 1 cut(s) 693
BstACI GRCGYC 2 cut(s) 464, 554
BstAFI CTTAAG 2 cut(s) 46, 584
BstAUI TGTACA 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 722
BstC8I GCNNGC 6 cut(s) 184, 270, 404, 462, 648, 749
BstDEI CTNAG 2 cut(s) 521, 530
BstDSI CCRYGG 3 cut(s) 31, 346, 557
BstF5I GGATG 3 cut(s) 126, 394, 661
BstFNI CGCG 5 cut(s) 279, 315, 336, 353, 509
BstH2I RGCGCY 4 cut(s) 461, 467, 530, 557
BstHHI GCGC 7 cut(s) 336, 353, 460, 466, 509, 529, 556
BstKTI GATC 2 cut(s) 38, 89
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 35, 86
BstMWI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
BstNI CCWGG 2 cut(s) 91, 667
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 7 cut(s) 89, 253, 254, 263, 302, 378, 665
BstUI CGCG 5 cut(s) 279, 315, 336, 353, 509
BstV1I GCAGC 2 cut(s) 694, 763
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 5 cut(s) 144, 186, 339, 670, 728
BtgI CCRYGG 3 cut(s) 31, 346, 557
BtgZI GCGATG 1 cut(s) 163
BtrI CACGTC 2 cut(s) 322, 619
BtsCI GGATG 3 cut(s) 126, 394, 661
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 6 cut(s) 184, 270, 404, 462, 648, 749
CfoI GCGC 7 cut(s) 336, 353, 460, 466, 509, 529, 556
Cfr10I RCCGGY 2 cut(s) 182, 710
Cfr13I GGNCC 3 cut(s) 185, 258, 286
Cfr9I CCCGGG 1 cut(s) 254
CseI GACGC 3 cut(s) 268, 304, 520
Csp6I GTAC 4 cut(s) 232, 244, 376, 759
CviAII CATG 7 cut(s) 32, 229, 367, 373, 647, 692, 740
CviQI GTAC 4 cut(s) 232, 244, 376, 759
DdeI CTNAG 2 cut(s) 521, 530
DinI GGCGCC 2 cut(s) 465, 555
DpnI GATC 2 cut(s) 37, 88
DpnII GATC 2 cut(s) 35, 86
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EciI GGCGGA 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 258, 286
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 2 cut(s) 258, 286
EcoRI GAATTC 1 cut(s) 718
EcoRII CCWGG 2 cut(s) 89, 665
EcoT14I CCWWGG 1 cut(s) 31
EgeI GGCGCC 2 cut(s) 465, 555
EheI GGCGCC 2 cut(s) 465, 555
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
FaqI GGGAC 3 cut(s) 146, 244, 440
FatI CATG 7 cut(s) 31, 228, 366, 372, 646, 691, 739
FauI CCCGC 4 cut(s) 76, 256, 284, 320
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 4 cut(s) 300, 337, 708, 752
FokI GGATG 3 cut(s) 113, 401, 648
Fsp4HI GCNGC 4 cut(s) 300, 337, 708, 752
GlaI GCGC 7 cut(s) 335, 352, 459, 465, 508, 528, 555
GluI GCNGC 4 cut(s) 300, 337, 708, 752
HaeII RGCGCY 4 cut(s) 461, 467, 530, 557
HaeIII GGCC 5 cut(s) 144, 186, 339, 670, 728
HapII CCGG 9 cut(s) 72, 141, 183, 255, 265, 303, 380, 395, 711
HgaI GACGC 3 cut(s) 268, 304, 520
HhaI GCGC 7 cut(s) 336, 353, 460, 466, 509, 529, 556
Hin1I GRCGYC 2 cut(s) 464, 554
Hin1II CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
Hin6I GCGC 7 cut(s) 334, 351, 458, 464, 507, 527, 554
HinP1I GCGC 7 cut(s) 334, 351, 458, 464, 507, 527, 554
HincII GTYRAC 3 cut(s) 172, 319, 597
HindII GTYRAC 3 cut(s) 172, 319, 597
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 1 cut(s) 598
HpaII CCGG 9 cut(s) 72, 141, 183, 255, 265, 303, 380, 395, 711
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 6 cut(s) 172, 319, 325, 597, 622, 786
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 6 cut(s) 172, 319, 325, 597, 622, 786
Hpy99I CGWCG 3 cut(s) 320, 323, 514
HpyAV CCTTC 2 cut(s) 448, 718
HpyCH4III ACNGT 2 cut(s) 78, 169
HpyCH4IV ACGT 3 cut(s) 17, 321, 618
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
HpyF3I CTNAG 2 cut(s) 521, 530
HpySE526I ACGT 3 cut(s) 17, 321, 618
Hsp92I GRCGYC 2 cut(s) 464, 554
Hsp92II CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
HspAI GCGC 7 cut(s) 334, 351, 458, 464, 507, 527, 554
KasI GGCGCC 2 cut(s) 463, 553
KflI GGGWCCC 1 cut(s) 258
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 35, 86
LmnI GCTCC 3 cut(s) 432, 504, 646
Lsp1109I GCAGC 2 cut(s) 694, 763
MaeII ACGT 3 cut(s) 17, 321, 618
MalI GATC 2 cut(s) 37, 88
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 35, 86
MboII GAAGA 3 cut(s) 618, 626, 680
MflI RGATCY 1 cut(s) 35
MluCI AATT 1 cut(s) 718
Mly113I GGCGCC 2 cut(s) 464, 554
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 1 cut(s) 546
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 2 cut(s) 47, 585
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 406
MspCI CTTAAG 2 cut(s) 46, 584
MspI CCGG 9 cut(s) 72, 141, 183, 255, 265, 303, 380, 395, 711
MspR9I CCNGG 7 cut(s) 91, 255, 256, 265, 304, 380, 667
MvaI CCWGG 2 cut(s) 91, 667
MvnI CGCG 5 cut(s) 279, 315, 336, 353, 509
MwoI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
NaeI GCCGGC 1 cut(s) 184
NarI GGCGCC 2 cut(s) 464, 554
NciI CCSGG 5 cut(s) 255, 256, 265, 304, 380
NcoI CCATGG 1 cut(s) 31
NdeII GATC 2 cut(s) 35, 86
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
NlaIV GGNNCC 8 cut(s) 37, 107, 259, 260, 308, 360, 465, 555
NspI RCATGY 1 cut(s) 650
NspV TTCGAA 1 cut(s) 722
PaeI GCATGC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 506
PdiI GCCGGC 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 4 cut(s) 301, 338, 709, 753
PleI GAGTC 1 cut(s) 592
PluTI GGCGCC 2 cut(s) 467, 557
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 2 cut(s) 258, 286
Psp5II RGGWCCY 2 cut(s) 258, 286
Psp6I CCWGG 2 cut(s) 89, 665
PspGI CCWGG 2 cut(s) 89, 665
PspN4I GGNNCC 8 cut(s) 37, 107, 259, 260, 308, 360, 465, 555
PspPI GGNCC 3 cut(s) 185, 258, 286
PspPPI RGGWCCY 2 cut(s) 258, 286
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 406
RsaI GTAC 4 cut(s) 233, 245, 377, 760
RsaNI GTAC 4 cut(s) 232, 244, 376, 759
RseI CAYNNNNRTG 1 cut(s) 371
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 47, 585
SatI GCNGC 4 cut(s) 300, 337, 708, 752
Sau3AI GATC 2 cut(s) 35, 86
Sau96I GGNCC 3 cut(s) 185, 258, 286
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 7 cut(s) 91, 255, 256, 265, 304, 380, 667
SfoI GGCGCC 2 cut(s) 465, 555
SfuI TTCGAA 1 cut(s) 722
SgrDI CGTCGACG 1 cut(s) 317
SinI GGWCC 2 cut(s) 258, 286
SmaI CCCGGG 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 4 cut(s) 46, 239, 584, 600
SmoI CTYRAG 4 cut(s) 46, 239, 584, 600
SphI GCATGC 1 cut(s) 650
Sse9I AATT 1 cut(s) 718
SspDI GGCGCC 2 cut(s) 463, 553
StyD4I CCNGG 7 cut(s) 89, 253, 254, 263, 302, 378, 665
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 78, 169
TaiI ACGT 3 cut(s) 20, 324, 621
TaqI TCGA 3 cut(s) 318, 342, 722
TasI AATT 1 cut(s) 718
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 302, 339
Tru1I TTAA 2 cut(s) 47, 585
Tru9I TTAA 2 cut(s) 47, 585
TscAI CASTG 1 cut(s) 152
TseI GCWGC 2 cut(s) 707, 751
TspDTI ATGAA 5 cut(s) 361, 627, 680, 756, 765
TspGWI ACGGA 3 cut(s) 148, 400, 554
TspMI CCCGGG 1 cut(s) 254
TspRI CASTG 1 cut(s) 152
Vha464I CTTAAG 2 cut(s) 46, 584
VpaK11BI GGWCC 2 cut(s) 258, 286
XapI RAATTY 1 cut(s) 718
XceI RCATGY 1 cut(s) 650
XmaI CCCGGG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.