Rh6BG094200

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005435
Physical Location & Seq
Forward (+)
14876488 .. 14876889
402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG094200.1

Sequence Viewer

Length: 402 bp
ATGGCAGTAATGGACACGTTCTACATCTCCCATGGATCCCCAACCCTAAGTATTGACGAGACCATTCCCGCCGGACAGTTCCTCCGATCCTGGAAACAAAACGGGTTCCCCCAGAAACCCAACTCCATCCTCATCATCTCCGGCCACTGGGAAACCTCCGTCCCCACCGTCAACTCCATCGCCGGCCCACACGACACCATCTACGACTTCTACGGCTTCCCCAAACCCATGTACAAGCTCAAGTACCCCGCCCCCGGGTCCCCCCAGCTCGCCTCCCGCGTCAAGGACCTCCTCACCGCAGCCGGGTTCCCCCGCGTCGACGTGGACACCAAGCGCGGCCTCGACCACGGCGCGTGGGTCCCGCTCATGTTCATGCAGGGACGGAGCTACTCAAGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

133

Amino Acids

14.79

Weight (kDa)

9.26

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 6 - 127 3.3e-22 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 318
AccII CGCG 4 cut(s) 279, 315, 336, 353
AciI CCGC 7 cut(s) 69, 249, 277, 297, 313, 336, 362
AclWI GGATC 3 cut(s) 30, 43, 81
AcoI YGGCCR 1 cut(s) 142
AfaI GTAC 2 cut(s) 233, 245
AfiI CCNNNNNNNGG 5 cut(s) 147, 254, 255, 283, 303
AflIII ACRYGT 1 cut(s) 15
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 3 cut(s) 238, 268, 387
AluI AGCT 3 cut(s) 238, 268, 387
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 30, 43, 81
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 4 cut(s) 142, 184, 337, 396
ApeKI GCWGC 1 cut(s) 299
AspLEI GCGC 2 cut(s) 336, 353
AspS9I GGNCC 5 cut(s) 185, 258, 286, 358, 396
AsuC2I CCSGG 3 cut(s) 255, 256, 304
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 3 cut(s) 258, 286, 358
BamHI GGATCC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 311
BccI CCATC 3 cut(s) 134, 185, 206
BceAI ACGGC 2 cut(s) 229, 364
BciT130I CCWGG 1 cut(s) 91
BcnI CCSGG 3 cut(s) 255, 256, 304
BcoDI GTCTC 1 cut(s) 53
BisI GCNGC 2 cut(s) 300, 337
BlsI GCNGC 2 cut(s) 301, 338
Bme1390I CCNGG 4 cut(s) 91, 255, 256, 304
Bme18I GGWCC 3 cut(s) 258, 286, 358
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 5 cut(s) 185, 258, 286, 358, 396
BmiI GGNNCC 7 cut(s) 37, 107, 259, 260, 308, 359, 360
BmrFI CCNGG 4 cut(s) 91, 255, 256, 304
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
BpuEI CTTGAG 2 cut(s) 224, 376
BpuMI CCSGG 3 cut(s) 255, 256, 304
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 4 cut(s) 31, 253, 254, 346
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 254, 255, 283, 303
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 4 cut(s) 31, 253, 254, 346
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 5 cut(s) 147, 254, 255, 283, 303
BseNI ACTGG 1 cut(s) 152
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 311
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 4 cut(s) 279, 315, 336, 353
BshFI GGCC 4 cut(s) 144, 186, 339, 398
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 5 cut(s) 72, 141, 183, 255, 303
BslFI GGGAC 4 cut(s) 146, 244, 344, 393
BslI CCNNNNNNNGG 5 cut(s) 147, 254, 255, 283, 303
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 4 cut(s) 146, 244, 344, 393
BsnI GGCC 4 cut(s) 144, 186, 339, 398
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 35, 86
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 7 cut(s) 69, 249, 277, 297, 313, 336, 362
BspANI GGCC 4 cut(s) 144, 186, 339, 398
BspFNI CGCG 4 cut(s) 279, 315, 336, 353
BspLI GGNNCC 7 cut(s) 37, 107, 259, 260, 308, 359, 360
BspPI GGATC 3 cut(s) 30, 43, 81
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 31, 253, 254, 346
BssMI GATC 2 cut(s) 35, 86
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 2 cut(s) 78, 169
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 184, 270
BstDEI CTNAG 1 cut(s) 47
BstDSI CCRYGG 2 cut(s) 31, 346
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 4 cut(s) 279, 315, 336, 353
BstHHI GCGC 2 cut(s) 336, 353
BstKTI GATC 2 cut(s) 38, 89
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 35, 86
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 4 cut(s) 89, 253, 254, 302
BstUI CGCG 4 cut(s) 279, 315, 336, 353
BstV1I GCAGC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 4 cut(s) 144, 186, 339, 398
BtgI CCRYGG 2 cut(s) 31, 346
BtgZI GCGATG 1 cut(s) 163
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 184, 270
CfoI GCGC 2 cut(s) 336, 353
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 185, 258, 286, 358, 396
Cfr9I CCCGGG 1 cut(s) 254
CseI GACGC 2 cut(s) 268, 304
Csp6I GTAC 2 cut(s) 232, 244
CviAII CATG 4 cut(s) 32, 229, 367, 373
CviJI RGCY 9 cut(s) 144, 186, 216, 238, 268, 302, 339, 387, 398
CviKI_1 RGCY 9 cut(s) 144, 186, 216, 238, 268, 302, 339, 387, 398
CviQI GTAC 2 cut(s) 232, 244
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 37, 88
DpnII GATC 2 cut(s) 35, 86
EaeI YGGCCR 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 31
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 3 cut(s) 258, 286, 358
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 4 cut(s) 258, 286, 358, 396
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 4 cut(s) 35, 232, 370, 376
FaiI YATR 4 cut(s) 33, 230, 368, 374
FaqI GGGAC 4 cut(s) 146, 244, 344, 393
FatI CATG 4 cut(s) 31, 228, 366, 372
FauI CCCGC 5 cut(s) 76, 256, 284, 320, 369
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 2 cut(s) 300, 337
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 300, 337
GlaI GCGC 2 cut(s) 335, 352
GluI GCNGC 2 cut(s) 300, 337
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 4 cut(s) 144, 186, 339, 398
HapII CCGG 5 cut(s) 72, 141, 183, 255, 303
HgaI GACGC 2 cut(s) 268, 304
HhaI GCGC 2 cut(s) 336, 353
Hin1II CATG 4 cut(s) 35, 232, 370, 376
Hin6I GCGC 2 cut(s) 334, 351
HinP1I GCGC 2 cut(s) 334, 351
HincII GTYRAC 2 cut(s) 172, 319
HindII GTYRAC 2 cut(s) 172, 319
HpaII CCGG 5 cut(s) 72, 141, 183, 255, 303
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 3 cut(s) 172, 319, 325
Hpy188I TCNGA 1 cut(s) 86
Hpy8I GTNNAC 3 cut(s) 172, 319, 325
Hpy99I CGWCG 2 cut(s) 320, 323
HpyCH4III ACNGT 2 cut(s) 78, 169
HpyCH4IV ACGT 2 cut(s) 17, 321
HpyCH4V TGCA 1 cut(s) 376
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 2 cut(s) 17, 321
Hsp92II CATG 4 cut(s) 35, 232, 370, 376
HspAI GCGC 2 cut(s) 334, 351
KflI GGGWCCC 2 cut(s) 258, 358
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 35, 86
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 1 cut(s) 311
MaeII ACGT 2 cut(s) 17, 321
MalI GATC 2 cut(s) 37, 88
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 35, 86
MflI RGATCY 1 cut(s) 35
MnlI CCTC 7 cut(s) 92, 140, 166, 283, 299, 302, 350
MroNI GCCGGC 1 cut(s) 182
MslI CAYNNNNRTG 1 cut(s) 371
MspI CCGG 5 cut(s) 72, 141, 183, 255, 303
MspR9I CCNGG 4 cut(s) 91, 255, 256, 304
MvaI CCWGG 1 cut(s) 91
MvnI CGCG 4 cut(s) 279, 315, 336, 353
NaeI GCCGGC 1 cut(s) 184
NciI CCSGG 3 cut(s) 255, 256, 304
NcoI CCATGG 1 cut(s) 31
NdeII GATC 2 cut(s) 35, 86
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 4 cut(s) 35, 232, 370, 376
NlaIV GGNNCC 7 cut(s) 37, 107, 259, 260, 308, 359, 360
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 184
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 2 cut(s) 301, 338
PpuMI RGGWCCY 3 cut(s) 258, 286, 358
Psp5II RGGWCCY 3 cut(s) 258, 286, 358
Psp6I CCWGG 1 cut(s) 89
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 1 cut(s) 89
PspN4I GGNNCC 7 cut(s) 37, 107, 259, 260, 308, 359, 360
PspPI GGNCC 5 cut(s) 185, 258, 286, 358, 396
PspPPI RGGWCCY 3 cut(s) 258, 286, 358
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 2 cut(s) 233, 245
RsaNI GTAC 2 cut(s) 232, 244
RseI CAYNNNNRTG 1 cut(s) 371
SalI GTCGAC 1 cut(s) 317
SatI GCNGC 2 cut(s) 300, 337
Sau3AI GATC 2 cut(s) 35, 86
Sau96I GGNCC 5 cut(s) 185, 258, 286, 358, 396
ScrFI CCNGG 4 cut(s) 91, 255, 256, 304
SetI ASST 7 cut(s) 20, 158, 240, 270, 291, 324, 389
SgrDI CGTCGACG 1 cut(s) 317
SinI GGWCC 3 cut(s) 258, 286, 358
SmaI CCCGGG 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 2 cut(s) 239, 391
SmoI CTYRAG 2 cut(s) 239, 391
SsiI CCGC 7 cut(s) 69, 249, 277, 297, 313, 336, 362
StyD4I CCNGG 4 cut(s) 89, 253, 254, 302
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 78, 169
TaiI ACGT 2 cut(s) 20, 324
TaqI TCGA 2 cut(s) 318, 342
TatI WGTACW 1 cut(s) 231
TauI GCSGC 1 cut(s) 339
TscAI CASTG 1 cut(s) 152
TseI GCWGC 1 cut(s) 299
TspDTI ATGAA 1 cut(s) 361
TspGWI ACGGA 2 cut(s) 148, 397
TspMI CCCGGG 1 cut(s) 254
TspRI CASTG 1 cut(s) 152
VpaK11BI GGWCC 3 cut(s) 258, 286, 358
XmaI CCCGGG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.