Rroxscaffold_2G00124060

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005435
Physical Location & Seq
Forward (+)
58139883 .. 58140677
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124060.1

Sequence Viewer

Length: 795 bp
ATGGCGGTAATGGACACCTTCTACATCTCCCATGGATCCCCAACTCTAAGTATTGACGAGACCATTCCCGCCGGGCAGTTCCTCCGATCCTGGAAACAAAACGGGTTCCCCCAGAAACCTAACTCCATCCTTGTCATCTCCGGCCACTGGGAAACCTCCGTCCCCACCGTCAACTCCATCTCCGGCCCACACGACACCATCTACGACTTCTACGGCTTCCCCAAACCCATGTACAAGCTCAAGTACCCCGCCCCCGGGTCCCCCCAGCTCGCCTCCCGCGTCAAGGACCTCCTCACAGCCGCCGGGTTCCCCCGCGTCGACGTGGACACCAAGCGCGGGCTCGACCACGGCGCGTGGGTCCCGCTCATGTTCATGTACCCGGAGGCGGATATTCCGGTGTGCCAGCTGTCCGTCCAGACTAACAGAGACGGGAGCTATCACTACGATATGGGGAAGGCGCTGGCGCCGTTGAGGGAGGAGGGTGTCCTTATTGTGGGGTCGGGGAGCGCGACGCATAATCTAAGGTCGCTGAGGAACACGGACGGGGTGGTGGCGCCGTGGGCATTGGAGTTTGATACTTGGCTTAAGGAGGCGCTGACTCAAGGAAGATATGAAGATGTGAACCATTATGAGGAGAAAGCTCCGCATGCGAAAACGGCTCATCCCTGGCCTGACCATTTCTACCCTCTTCATGTGGCAATGGGGGCTGCCGGTGCGAATTCCAAGGCCAAGCTCATCCATGAAAGCTGGCAGCTTAGTACTCTTTCTTATGCTTCGTACCAGTTTACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

264

Amino Acids

29.36

Weight (kDa)

6.29

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 6 - 262 2.5e-53 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 463, 553
AccBSI CCGCTC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 318
AccII CGCG 5 cut(s) 279, 315, 336, 353, 509
AclWI GGATC 3 cut(s) 30, 43, 81
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 1 cut(s) 718
AcyI GRCGYC 2 cut(s) 464, 554
AfaI GTAC 5 cut(s) 233, 245, 377, 760, 779
AfiI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 336, 385, 493, 631
AflII CTTAAG 1 cut(s) 584
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 89, 665
AluBI AGCT 8 cut(s) 238, 268, 406, 435, 641, 733, 747, 754
AluI AGCT 8 cut(s) 238, 268, 406, 435, 641, 733, 747, 754
Alw26I GTCTC 2 cut(s) 53, 420
AlwI GGATC 3 cut(s) 30, 43, 81
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 4 cut(s) 142, 184, 668, 726
ApeKI GCWGC 2 cut(s) 707, 751
ApoI RAATTY 1 cut(s) 718
AspLEI GCGC 7 cut(s) 336, 353, 460, 466, 509, 556, 595
AspS9I GGNCC 4 cut(s) 185, 258, 286, 358
AsuC2I CCSGG 5 cut(s) 73, 255, 256, 304, 380
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 3 cut(s) 258, 286, 358
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 2 cut(s) 463, 553
BanII GRGCYC 1 cut(s) 342
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 2 cut(s) 694, 763
BccI CCATC 3 cut(s) 134, 185, 206
BceAI ACGGC 5 cut(s) 229, 364, 451, 541, 672
BciT130I CCWGG 2 cut(s) 91, 667
BcnI CCSGG 5 cut(s) 73, 255, 256, 304, 380
BcoDI GTCTC 2 cut(s) 53, 420
BfoI RGCGCY 4 cut(s) 461, 467, 557, 596
BfrI CTTAAG 1 cut(s) 584
BisI GCNGC 3 cut(s) 300, 708, 752
BlsI GCNGC 3 cut(s) 301, 709, 753
BmcAI AGTACT 1 cut(s) 760
Bme1390I CCNGG 7 cut(s) 73, 91, 255, 256, 304, 380, 667
Bme18I GGWCC 3 cut(s) 258, 286, 358
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 4 cut(s) 185, 258, 286, 358
BmiI GGNNCC 9 cut(s) 37, 107, 259, 260, 308, 359, 360, 465, 555
BmrFI CCNGG 7 cut(s) 73, 91, 255, 256, 304, 380, 667
BmrI ACTGGG 1 cut(s) 157
BmuI ACTGGG 1 cut(s) 157
Bpu10I CCTNAGC 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 224, 585
BpuMI CCSGG 5 cut(s) 73, 255, 256, 304, 380
BsaHI GRCGYC 2 cut(s) 464, 554
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 7 cut(s) 31, 253, 254, 346, 557, 665, 723
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 336, 385, 493, 631
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 3 cut(s) 152, 781, 789
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 2 cut(s) 91, 667
BseDI CCNNGG 7 cut(s) 31, 253, 254, 346, 557, 665, 723
BseGI GGATG 3 cut(s) 126, 661, 735
BseLI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 336, 385, 493, 631
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 3 cut(s) 152, 781, 789
BseRI GAGGAG 3 cut(s) 281, 491, 647
BseXI GCAGC 2 cut(s) 694, 763
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 5 cut(s) 279, 315, 336, 353, 509
BshFI GGCC 4 cut(s) 144, 186, 670, 728
BshNI GGYRCC 2 cut(s) 463, 553
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 8 cut(s) 72, 141, 183, 255, 303, 380, 395, 711
BslFI GGGAC 3 cut(s) 146, 244, 344
BslI CCNNNNNNNGG 8 cut(s) 147, 254, 255, 283, 336, 385, 493, 631
BsmAI GTCTC 2 cut(s) 53, 420
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 3 cut(s) 146, 244, 344
BsnI GGCC 4 cut(s) 144, 186, 670, 728
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 35, 86
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 4 cut(s) 144, 186, 670, 728
BspCNI CTCAG 1 cut(s) 522
BspFNI CGCG 5 cut(s) 279, 315, 336, 353, 509
BspLI GGNNCC 9 cut(s) 37, 107, 259, 260, 308, 359, 360, 465, 555
BspPI GGATC 3 cut(s) 30, 43, 81
BspT107I GGYRCC 2 cut(s) 463, 553
BspTI CTTAAG 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 710
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 3 cut(s) 152, 781, 789
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 7 cut(s) 31, 253, 254, 346, 557, 665, 723
BssMI GATC 2 cut(s) 35, 86
BssNI GRCGYC 2 cut(s) 464, 554
BssT1I CCWWGG 2 cut(s) 31, 723
Bst2UI CCWGG 2 cut(s) 91, 667
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 693
BstACI GRCGYC 2 cut(s) 464, 554
BstAFI CTTAAG 1 cut(s) 584
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 6 cut(s) 270, 338, 404, 462, 648, 749
BstDEI CTNAG 4 cut(s) 47, 521, 530, 755
BstDSI CCRYGG 3 cut(s) 31, 346, 557
BstF5I GGATG 3 cut(s) 126, 661, 735
BstFNI CGCG 5 cut(s) 279, 315, 336, 353, 509
BstH2I RGCGCY 4 cut(s) 461, 467, 557, 596
BstHHI GCGC 7 cut(s) 336, 353, 460, 466, 509, 556, 595
BstKTI GATC 2 cut(s) 38, 89
BstMAI GTCTC 2 cut(s) 53, 420
BstMBI GATC 2 cut(s) 35, 86
BstMWI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
BstNI CCWGG 2 cut(s) 91, 667
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 7 cut(s) 71, 89, 253, 254, 302, 378, 665
BstUI CGCG 5 cut(s) 279, 315, 336, 353, 509
BstV1I GCAGC 2 cut(s) 694, 763
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 4 cut(s) 144, 186, 670, 728
BtgI CCRYGG 3 cut(s) 31, 346, 557
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 3 cut(s) 126, 661, 735
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 6 cut(s) 270, 338, 404, 462, 648, 749
CfoI GCGC 7 cut(s) 336, 353, 460, 466, 509, 556, 595
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 4 cut(s) 185, 258, 286, 358
Cfr9I CCCGGG 1 cut(s) 254
CseI GACGC 3 cut(s) 268, 304, 520
Csp6I GTAC 5 cut(s) 232, 244, 376, 759, 778
CviAII CATG 7 cut(s) 32, 229, 367, 373, 647, 692, 740
CviQI GTAC 5 cut(s) 232, 244, 376, 759, 778
DdeI CTNAG 4 cut(s) 47, 521, 530, 755
DinI GGCGCC 2 cut(s) 465, 555
DpnI GATC 2 cut(s) 37, 88
DpnII GATC 2 cut(s) 35, 86
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 693
EarI CTCTTC 1 cut(s) 693
EciI GGCGGA 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 31, 723
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 53
Eco47I GGWCC 3 cut(s) 258, 286, 358
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 3 cut(s) 258, 286, 358
EcoRI GAATTC 1 cut(s) 718
EcoRII CCWGG 2 cut(s) 89, 665
EcoT14I CCWWGG 2 cut(s) 31, 723
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 2 cut(s) 465, 555
EheI GGCGCC 2 cut(s) 465, 555
ErhI CCWWGG 2 cut(s) 31, 723
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
FaqI GGGAC 3 cut(s) 146, 244, 344
FatI CATG 7 cut(s) 31, 228, 366, 372, 646, 691, 739
FauI CCCGC 6 cut(s) 76, 256, 284, 320, 329, 369
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 3 cut(s) 300, 708, 752
FokI GGATG 3 cut(s) 113, 648, 722
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 3 cut(s) 300, 708, 752
GlaI GCGC 7 cut(s) 335, 352, 459, 465, 508, 555, 594
GluI GCNGC 3 cut(s) 300, 708, 752
GsaI CCCAGC 1 cut(s) 268
HaeII RGCGCY 4 cut(s) 461, 467, 557, 596
HaeIII GGCC 4 cut(s) 144, 186, 670, 728
HapII CCGG 8 cut(s) 72, 141, 183, 255, 303, 380, 395, 711
HgaI GACGC 3 cut(s) 268, 304, 520
HhaI GCGC 7 cut(s) 336, 353, 460, 466, 509, 556, 595
Hin1I GRCGYC 2 cut(s) 464, 554
Hin1II CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
Hin6I GCGC 7 cut(s) 334, 351, 458, 464, 507, 554, 593
HinP1I GCGC 7 cut(s) 334, 351, 458, 464, 507, 554, 593
HincII GTYRAC 2 cut(s) 172, 319
HindII GTYRAC 2 cut(s) 172, 319
HinfI GANTC 1 cut(s) 598
HpaII CCGG 8 cut(s) 72, 141, 183, 255, 303, 380, 395, 711
Hpy166II GTNNAC 5 cut(s) 172, 319, 325, 622, 786
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 5 cut(s) 172, 319, 325, 622, 786
Hpy99I CGWCG 3 cut(s) 320, 323, 514
HpyAV CCTTC 2 cut(s) 28, 448
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 321
HpyF10VI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
HpyF3I CTNAG 4 cut(s) 47, 521, 530, 755
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 2 cut(s) 464, 554
Hsp92II CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
HspAI GCGC 7 cut(s) 334, 351, 458, 464, 507, 554, 593
KasI GGCGCC 2 cut(s) 463, 553
KflI GGGWCCC 2 cut(s) 258, 358
Kzo9I GATC 2 cut(s) 35, 86
LmnI GCTCC 3 cut(s) 432, 504, 646
Lsp1109I GCAGC 2 cut(s) 694, 763
MaeII ACGT 1 cut(s) 321
MalI GATC 2 cut(s) 37, 88
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 35, 86
MboII GAAGA 3 cut(s) 618, 626, 680
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 342
MluCI AATT 1 cut(s) 718
Mly113I GGCGCC 2 cut(s) 464, 554
MlyI GAGTC 1 cut(s) 592
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 406
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 8 cut(s) 72, 141, 183, 255, 303, 380, 395, 711
MspR9I CCNGG 7 cut(s) 73, 91, 255, 256, 304, 380, 667
MvaI CCWGG 2 cut(s) 91, 667
MvnI CGCG 5 cut(s) 279, 315, 336, 353, 509
MwoI GCNNNNNNNGC 5 cut(s) 560, 647, 656, 704, 713
NarI GGCGCC 2 cut(s) 464, 554
NciI CCSGG 5 cut(s) 73, 255, 256, 304, 380
NcoI CCATGG 1 cut(s) 31
NdeII GATC 2 cut(s) 35, 86
NlaIII CATG 7 cut(s) 35, 232, 370, 376, 650, 695, 743
NlaIV GGNNCC 9 cut(s) 37, 107, 259, 260, 308, 359, 360, 465, 555
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 506
PfoI TCCNGGA 1 cut(s) 89
PkrI GCNGC 3 cut(s) 301, 709, 753
PleI GAGTC 1 cut(s) 592
PluTI GGCGCC 2 cut(s) 467, 557
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 3 cut(s) 258, 286, 358
Psp5II RGGWCCY 3 cut(s) 258, 286, 358
Psp6I CCWGG 2 cut(s) 89, 665
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 89, 665
PspN4I GGNNCC 9 cut(s) 37, 107, 259, 260, 308, 359, 360, 465, 555
PspPI GGNCC 4 cut(s) 185, 258, 286, 358
PspPPI RGGWCCY 3 cut(s) 258, 286, 358
PsuI RGATCY 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 406
RsaI GTAC 5 cut(s) 233, 245, 377, 760, 779
RsaNI GTAC 5 cut(s) 232, 244, 376, 759, 778
RseI CAYNNNNRTG 1 cut(s) 371
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 3 cut(s) 300, 708, 752
Sau3AI GATC 2 cut(s) 35, 86
Sau96I GGNCC 4 cut(s) 185, 258, 286, 358
ScaI AGTACT 1 cut(s) 760
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 7 cut(s) 73, 91, 255, 256, 304, 380, 667
SduI GDGCHC 1 cut(s) 342
SfoI GGCGCC 2 cut(s) 465, 555
SgrDI CGTCGACG 1 cut(s) 317
SinI GGWCC 3 cut(s) 258, 286, 358
SmaI CCCGGG 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 3 cut(s) 239, 584, 600
SmoI CTYRAG 3 cut(s) 239, 584, 600
SphI GCATGC 1 cut(s) 650
Sse9I AATT 1 cut(s) 718
SspDI GGCGCC 2 cut(s) 463, 553
StyD4I CCNGG 7 cut(s) 71, 89, 253, 254, 302, 378, 665
StyI CCWWGG 2 cut(s) 31, 723
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 324
TaqI TCGA 2 cut(s) 318, 342
TasI AATT 1 cut(s) 718
TatI WGTACW 2 cut(s) 231, 758
TauI GCSGC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 152
TseI GCWGC 2 cut(s) 707, 751
TspDTI ATGAA 4 cut(s) 361, 627, 680, 756
TspGWI ACGGA 3 cut(s) 148, 400, 554
TspMI CCCGGG 1 cut(s) 254
TspRI CASTG 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 584
VpaK11BI GGWCC 3 cut(s) 258, 286, 358
XapI RAATTY 1 cut(s) 718
XceI RCATGY 1 cut(s) 650
XmaI CCCGGG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.