Rh2AG281900

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005435
Physical Location & Seq
Reverse (-)
34214398 .. 34214868
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG281900.1

Sequence Viewer

Length: 471 bp
ATGCCGATAATAGACACATTCTACATGTCGCACGGATCCCCAACTCTAAGCATCGATGAGACCATACCTACCCGAAAGTTCCTCCAATCTTGGAAACAAAACGGGTTCCCCCAGAAACCCAACTCTATCCTCACCATCTCTACACACTGGGAAGCCTCCGTCCCCATATTCAACTCTATTGTCGGCCTAAACGACACCATCTACTACTTCTACGGCTTTCCCAACTCCCTGTACAAGCTCAAGTACCCTGCCCCAAGGTCACCCCAACTAGCCACCCGAGTCAAGGATCTTCTCACTGCTGCTGGATTCCCTAGCGTTGATGTGGACAACAAGCGCGACCTCAACCACGGTGCCTGGGTACCACTCAAGTTCATGTACCCGGAGGCAGACATCCTGCTGTGCCAGCTGTCCGTCCTGACCGATAGTGACAGGAGCTATCAGTTCAATTTGGGGAAGGCATTGGCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

156

Amino Acids

17.69

Weight (kDa)

7.84

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 6 - 155 2.6e-21 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 358
AccB1I GGYRCC 2 cut(s) 350, 358
AccII CGCG 1 cut(s) 336
AclWI GGATC 3 cut(s) 30, 43, 294
AfaI GTAC 4 cut(s) 233, 245, 360, 377
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 2 cut(s) 172, 445
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 3 cut(s) 238, 406, 435
AluI AGCT 3 cut(s) 238, 406, 435
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 30, 43, 294
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 299
Asp718I GGTACC 1 cut(s) 358
AspLEI GCGC 1 cut(s) 336
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 124, 252
AvaI CYCGRG 1 cut(s) 276
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 2 cut(s) 350, 358
BbvI GCAGC 1 cut(s) 286
BccI CCATC 2 cut(s) 143, 206
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 269, 312
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
Bme1390I CCNGG 2 cut(s) 355, 380
BmeT110I CYCGRG 1 cut(s) 276
BmiI GGNNCC 4 cut(s) 37, 107, 352, 360
BmrFI CCNGG 2 cut(s) 355, 380
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 157
BpuEI CTTGAG 2 cut(s) 224, 350
BpuMI CCSGG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 3 cut(s) 254, 346, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 3 cut(s) 254, 346, 354
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 283
BseNI ACTGG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 286
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 186
BshNI GGYRCC 2 cut(s) 350, 358
BshVI ATCGAT 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 186
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 1 cut(s) 276
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 35, 286
BspANI GGCC 1 cut(s) 186
BspDI ATCGAT 1 cut(s) 54
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 4 cut(s) 37, 107, 352, 360
BspPI GGATC 3 cut(s) 30, 43, 294
BspT107I GGYRCC 2 cut(s) 350, 358
BspTNI GGTCTC 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 1 cut(s) 152
BssECI CCNNGG 3 cut(s) 254, 346, 354
BssMI GATC 2 cut(s) 35, 286
BssT1I CCWWGG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 47
BstDSI CCRYGG 1 cut(s) 346
BstEII GGTNACC 1 cut(s) 258
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 2 cut(s) 38, 289
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 2 cut(s) 35, 286
BstMWI GCNNNNNNNGC 1 cut(s) 403
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 28
BstPI GGTNACC 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 353, 378
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 286
BstX2I RGATCY 2 cut(s) 35, 286
BstYI RGATCY 2 cut(s) 35, 286
Bsu15I ATCGAT 1 cut(s) 54
BsuRI GGCC 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 346
BtsCI GGATG 1 cut(s) 390
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 145, 294
Cac8I GCNNGC 1 cut(s) 404
CfoI GCGC 1 cut(s) 336
ClaI ATCGAT 1 cut(s) 54
Csp6I GTAC 4 cut(s) 232, 244, 359, 376
CviAII CATG 2 cut(s) 25, 373
CviJI RGCY 7 cut(s) 155, 186, 216, 238, 272, 406, 435
CviKI_1 RGCY 7 cut(s) 155, 186, 216, 238, 272, 406, 435
CviQI GTAC 4 cut(s) 232, 244, 359, 376
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 37, 288
DpnII GATC 2 cut(s) 35, 286
Eco130I CCWWGG 1 cut(s) 254
Eco31I GGTCTC 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 2 cut(s) 28, 376
FaiI YATR 4 cut(s) 26, 65, 167, 374
FaqI GGGAC 1 cut(s) 146
FatI CATG 2 cut(s) 24, 372
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 2 cut(s) 269, 312
GlaI GCGC 1 cut(s) 335
GluI GCNGC 1 cut(s) 300
HaeIII GGCC 1 cut(s) 186
HapII CCGG 1 cut(s) 380
HhaI GCGC 1 cut(s) 336
Hin1II CATG 2 cut(s) 28, 376
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HinfI GANTC 2 cut(s) 279, 306
HpaII CCGG 1 cut(s) 380
HphI GGTGA 2 cut(s) 124, 252
Hpy166II GTNNAC 1 cut(s) 325
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 448
HpyCH4III ACNGT 1 cut(s) 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 403
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 2 cut(s) 28, 376
HspAI GCGC 1 cut(s) 334
KpnI GGTACC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 35, 286
LmnI GCTCC 1 cut(s) 432
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 1 cut(s) 60
MaeI CTAG 2 cut(s) 269, 312
MaeIII GTNAC 2 cut(s) 258, 425
MalI GATC 2 cut(s) 37, 288
MboI GATC 2 cut(s) 35, 286
MboII GAAGA 1 cut(s) 281
MflI RGATCY 2 cut(s) 35, 286
MluCI AATT 1 cut(s) 445
MlyI GAGTC 1 cut(s) 288
MnlI CCTC 5 cut(s) 92, 140, 166, 350, 376
MseI TTAA 1 cut(s) 469
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 2 cut(s) 355, 380
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 403
NciI CCSGG 1 cut(s) 380
NdeII GATC 2 cut(s) 35, 286
NlaIII CATG 2 cut(s) 28, 376
NlaIV GGNNCC 4 cut(s) 37, 107, 352, 360
NmuCI GTSAC 2 cut(s) 258, 425
NspI RCATGY 1 cut(s) 28
PciI ACATGT 1 cut(s) 24
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 1 cut(s) 306
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PscI ACATGT 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 353
PspEI GGTNACC 1 cut(s) 258
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 4 cut(s) 37, 107, 352, 360
PsuI RGATCY 2 cut(s) 35, 286
PvuII CAGCTG 1 cut(s) 406
RsaI GTAC 4 cut(s) 233, 245, 360, 377
RsaNI GTAC 4 cut(s) 232, 244, 359, 376
SaqAI TTAA 1 cut(s) 469
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 2 cut(s) 35, 286
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 355, 380
SetI ASST 6 cut(s) 70, 240, 260, 342, 408, 437
SfaNI GCATC 1 cut(s) 60
SmlI CTYRAG 2 cut(s) 239, 365
SmoI CTYRAG 2 cut(s) 239, 365
Sse9I AATT 1 cut(s) 445
SspMI CTAG 2 cut(s) 269, 312
StyD4I CCNGG 2 cut(s) 353, 378
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 54
TaqII GACCGA 1 cut(s) 434
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TscAI CASTG 2 cut(s) 152, 301
TseFI GTSAC 2 cut(s) 258, 425
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 2 cut(s) 258, 425
TspDTI ATGAA 1 cut(s) 361
TspGWI ACGGA 3 cut(s) 48, 148, 400
TspRI CASTG 2 cut(s) 152, 301
XceI RCATGY 1 cut(s) 28
XspI CTAG 2 cut(s) 269, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.