MD00G1165300.v1.1

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005435
Physical Location & Seq
Reverse (-)
37621031 .. 37621837
807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1165300.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGCATTGAAGGACACGTTCTACCTATCGCACGGATCCCCCACGCTCAGCATCGACGAGTCGATTCCGGCCAGAAAATTCCTCCAGTCTTGGAAGGACACGGTCTATCCTCACAAACCCACCTCCATTCTCATCATCTCAGGACACTGGGAGACCGCTGTCCCCACCGTTAACTCCATTTCCGGCCGCCACGACACCATCTACGACTTCTACGGCTTCCCCAAATCCATGTACCAGATCAAGTACCCTGCCCCCGGCTCGCCCCACCTCTCTAACCGCGTCAAGCAGCTGCTTACGTCGTCCAGCTTCGGCCCCGCGGATGTGGACGGTAAGCGGGGGCTCGACCACGGCGCATGGGTCCCGCTCATGTTGATGTACCCGGAGGGAGACGTACCGGTTTGCCAGTTGTCGGTTCAGACAGAGAGGGACGCCACCTACCACTACAACATGGGGAGGGCGCTGGCTCCCCTTAAGGAGGAGGGCGTCCTTATAGTGGGGTCAGGCAGCGCCACTCACAACTTAAGGGCGTTGAAGAGGGAGACAAAGAGCAATGGATGTGCCGTCCCTTGGGCTCTCGAGTTTGACACATGGATCAAGGAAGCTCTTCTGGAAGGAAGGTACGAGGATGTGAACGAGTATGTGGAGAAGGCGCCGCATGCGAAAATGGCGCACCCTTGGCCGGATCACTTTTACCCGCTGCATGTAGCGGTGGGGGCTGCTGGTCCGGATGCAAAGGCTAAGCAAATCCACGACAGCTGGGGGCTCGGTGCTCTTTCGTATGCCTCCTATCAGTTTACATCGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

269

Amino Acids

29.74

Weight (kDa)

6.49

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 6 - 265 3.9e-50 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 649
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 2 cut(s) 279, 317
AccIII TCCGGA 1 cut(s) 724
AclWI GGATC 4 cut(s) 30, 43, 599, 690
AcoI YGGCCR 3 cut(s) 69, 184, 677
AcsI RAATTY 1 cut(s) 77
AcyI GRCGYC 3 cut(s) 429, 483, 650
AfaI GTAC 5 cut(s) 233, 245, 377, 393, 620
AfiI CCNNNNNNNGG 6 cut(s) 254, 409, 475, 493, 567, 679
AflII CTTAAG 2 cut(s) 470, 520
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 2 cut(s) 10, 532
AluBI AGCT 4 cut(s) 289, 306, 602, 756
AluI AGCT 4 cut(s) 289, 306, 602, 756
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 3 cut(s) 146, 381, 533
AlwI GGATC 4 cut(s) 30, 43, 599, 690
Ama87I CYCGRG 1 cut(s) 575
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 4 cut(s) 69, 184, 310, 677
ApeKI GCWGC 5 cut(s) 286, 289, 504, 697, 716
ApoI RAATTY 1 cut(s) 77
AsiGI ACCGGT 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 5 cut(s) 353, 460, 509, 652, 670
AspS9I GGNCC 3 cut(s) 311, 358, 722
AsuC2I CCSGG 2 cut(s) 255, 380
AvaI CYCGRG 1 cut(s) 575
AvaII GGWCC 2 cut(s) 358, 722
BamHI GGATCC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 649
BanII GRGCYC 3 cut(s) 342, 574, 765
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 5 cut(s) 276, 298, 516, 684, 703
BccI CCATC 1 cut(s) 206
BceAI ACGGC 3 cut(s) 229, 364, 545
BcnI CCSGG 2 cut(s) 255, 380
BcoDI GTCTC 3 cut(s) 146, 381, 533
BfoI RGCGCY 3 cut(s) 461, 510, 653
BfrI CTTAAG 2 cut(s) 470, 520
BisI GCNGC 7 cut(s) 187, 287, 290, 505, 653, 698, 717
BlpI GCTNAGC 2 cut(s) 47, 738
BlsI GCNGC 7 cut(s) 188, 288, 291, 506, 654, 699, 718
Bme1390I CCNGG 2 cut(s) 255, 380
Bme18I GGWCC 2 cut(s) 358, 722
BmeT110I CYCGRG 1 cut(s) 575
BmgT120I GGNCC 3 cut(s) 311, 358, 722
BmiI GGNNCC 6 cut(s) 37, 313, 359, 360, 465, 651
BmrFI CCNGG 2 cut(s) 255, 380
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 2 cut(s) 60, 718
BmuI ACTGGG 1 cut(s) 157
BoxI GACNNNNGTC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 68
Bpu1102I GCTNAGC 2 cut(s) 47, 738
BpuMI CCSGG 2 cut(s) 255, 380
BsaHI GRCGYC 3 cut(s) 429, 483, 650
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 5 cut(s) 253, 315, 346, 566, 674
BsaWI WCCGGW 2 cut(s) 394, 724
Bsc4I CCNNNNNNNGG 6 cut(s) 254, 409, 475, 493, 567, 679
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 3 cut(s) 85, 152, 403
Bse3DI GCAATG 1 cut(s) 556
BseAI TCCGGA 1 cut(s) 724
BseDI CCNNGG 5 cut(s) 253, 315, 346, 566, 674
BseGI GGATG 4 cut(s) 325, 560, 631, 733
BseLI CCNNNNNNNGG 6 cut(s) 254, 409, 475, 493, 567, 679
BseMI GCAATG 1 cut(s) 556
BseMII CTCAG 2 cut(s) 61, 153
BseNI ACTGG 3 cut(s) 85, 152, 403
BseRI GAGGAG 1 cut(s) 491
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 5 cut(s) 276, 298, 516, 684, 703
BseYI CCCAGC 1 cut(s) 756
Bsh1236I CGCG 2 cut(s) 279, 317
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 4 cut(s) 71, 186, 312, 679
BshNI GGYRCC 1 cut(s) 649
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 575
BsiSI CCGG 7 cut(s) 68, 183, 255, 380, 395, 680, 725
BslFI GGGAC 4 cut(s) 146, 344, 440, 548
BslI CCNNNNNNNGG 6 cut(s) 254, 409, 475, 493, 567, 679
BsmAI GTCTC 3 cut(s) 146, 381, 533
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 4 cut(s) 146, 344, 440, 548
BsnI GGCC 4 cut(s) 71, 186, 312, 679
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 575
Bsp1286I GDGCHC 4 cut(s) 342, 574, 765, 772
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 4 cut(s) 35, 237, 591, 682
Bsp1720I GCTNAGC 2 cut(s) 47, 738
BspANI GGCC 4 cut(s) 71, 186, 312, 679
BspCNI CTCAG 2 cut(s) 60, 152
BspEI TCCGGA 1 cut(s) 724
BspFNI CGCG 2 cut(s) 279, 317
BspLI GGNNCC 6 cut(s) 37, 313, 359, 360, 465, 651
BspPI GGATC 4 cut(s) 30, 43, 599, 690
BspQI GCTCTTC 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 649
BspTI CTTAAG 2 cut(s) 470, 520
BspTNI GGTCTC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 556
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 3 cut(s) 85, 152, 403
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 5 cut(s) 253, 315, 346, 566, 674
BssMI GATC 4 cut(s) 35, 237, 591, 682
BssNI GRCGYC 3 cut(s) 429, 483, 650
BssT1I CCWWGG 2 cut(s) 566, 674
Bst4CI ACNGT 3 cut(s) 103, 169, 329
Bst6I CTCTTC 2 cut(s) 527, 609
BstACI GRCGYC 3 cut(s) 429, 483, 650
BstAFI CTTAAG 2 cut(s) 470, 520
BstC8I GCNNGC 3 cut(s) 260, 462, 657
BstDEI CTNAG 3 cut(s) 47, 139, 738
BstDSI CCRYGG 2 cut(s) 315, 346
BstENI CCTNNNNNAGG 1 cut(s) 473
BstF5I GGATG 4 cut(s) 325, 560, 631, 733
BstFNI CGCG 2 cut(s) 279, 317
BstH2I RGCGCY 3 cut(s) 461, 510, 653
BstHHI GCGC 5 cut(s) 353, 460, 509, 652, 670
BstKTI GATC 4 cut(s) 38, 240, 594, 685
BstMAI GTCTC 3 cut(s) 146, 381, 533
BstMBI GATC 4 cut(s) 35, 237, 591, 682
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 4 cut(s) 656, 665, 676, 713
BstNSI RCATGY 2 cut(s) 659, 704
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 253, 378
BstUI CGCG 2 cut(s) 279, 317
BstV1I GCAGC 5 cut(s) 276, 298, 516, 684, 703
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BstZI CGGCCG 1 cut(s) 184
BsuRI GGCC 4 cut(s) 71, 186, 312, 679
BtgI CCRYGG 2 cut(s) 315, 346
BtgZI GCGATG 1 cut(s) 783
BtsCI GGATG 4 cut(s) 325, 560, 631, 733
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 260, 462, 657
CfoI GCGC 5 cut(s) 353, 460, 509, 652, 670
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 3 cut(s) 311, 358, 722
Cfr42I CCGCGG 1 cut(s) 318
CseI GACGC 3 cut(s) 268, 437, 472
Csp6I GTAC 5 cut(s) 232, 244, 376, 392, 619
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 7 cut(s) 229, 354, 367, 448, 588, 656, 701
CviQI GTAC 5 cut(s) 232, 244, 376, 392, 619
DdeI CTNAG 3 cut(s) 47, 139, 738
DinI GGCGCC 1 cut(s) 651
DpnI GATC 4 cut(s) 37, 239, 593, 684
DpnII GATC 4 cut(s) 35, 237, 591, 682
EaeI YGGCCR 3 cut(s) 69, 184, 677
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 527, 609
EarI CTCTTC 2 cut(s) 527, 609
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 566, 674
Eco24I GRGCYC 3 cut(s) 342, 574, 765
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 2 cut(s) 358, 722
Eco52I CGGCCG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 575
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 566, 674
EcoT38I GRGCYC 3 cut(s) 342, 574, 765
EgeI GGCGCC 1 cut(s) 651
EheI GGCGCC 1 cut(s) 651
ErhI CCWWGG 2 cut(s) 566, 674
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 7 cut(s) 232, 357, 370, 451, 591, 659, 704
FaqI GGGAC 4 cut(s) 146, 344, 440, 548
FatI CATG 7 cut(s) 228, 353, 366, 447, 587, 655, 700
FauI CCCGC 4 cut(s) 322, 327, 369, 702
Fnu4HI GCNGC 7 cut(s) 187, 287, 290, 505, 653, 698, 717
FokI GGATG 4 cut(s) 332, 567, 638, 740
FriOI GRGCYC 3 cut(s) 342, 574, 765
Fsp4HI GCNGC 7 cut(s) 187, 287, 290, 505, 653, 698, 717
GlaI GCGC 5 cut(s) 352, 459, 508, 651, 669
GluI GCNGC 7 cut(s) 187, 287, 290, 505, 653, 698, 717
GsaI CCCAGC 1 cut(s) 760
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 3 cut(s) 461, 510, 653
HaeIII GGCC 4 cut(s) 71, 186, 312, 679
HapII CCGG 7 cut(s) 68, 183, 255, 380, 395, 680, 725
HgaI GACGC 3 cut(s) 268, 437, 472
HhaI GCGC 5 cut(s) 353, 460, 509, 652, 670
Hin1I GRCGYC 3 cut(s) 429, 483, 650
Hin1II CATG 7 cut(s) 232, 357, 370, 451, 591, 659, 704
Hin6I GCGC 5 cut(s) 351, 458, 507, 650, 668
HinP1I GCGC 5 cut(s) 351, 458, 507, 650, 668
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 2 cut(s) 59, 64
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 7 cut(s) 68, 183, 255, 380, 395, 680, 725
Hpy166II GTNNAC 4 cut(s) 172, 325, 631, 795
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 4 cut(s) 141, 575, 608, 725
Hpy8I GTNNAC 4 cut(s) 172, 325, 631, 795
Hpy99I CGWCG 2 cut(s) 59, 301
HpyAV CCTTC 5 cut(s) 4, 88, 605, 609, 640
HpyCH4III ACNGT 3 cut(s) 103, 169, 329
HpyCH4IV ACGT 3 cut(s) 17, 296, 390
HpyCH4V TGCA 2 cut(s) 700, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 656, 665, 676, 713
HpyF3I CTNAG 3 cut(s) 47, 139, 738
HpySE526I ACGT 3 cut(s) 17, 296, 390
Hsp92I GRCGYC 3 cut(s) 429, 483, 650
Hsp92II CATG 7 cut(s) 232, 357, 370, 451, 591, 659, 704
HspAI GCGC 5 cut(s) 351, 458, 507, 650, 668
KasI GGCGCC 1 cut(s) 649
KflI GGGWCCC 1 cut(s) 358
Kpn2I TCCGGA 1 cut(s) 724
KspAI GTTAAC 1 cut(s) 172
KspI CCGCGG 1 cut(s) 318
Kzo9I GATC 4 cut(s) 35, 237, 591, 682
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 5 cut(s) 276, 298, 516, 684, 703
LweI GCATC 2 cut(s) 60, 718
MaeII ACGT 3 cut(s) 17, 296, 390
MalI GATC 4 cut(s) 37, 239, 593, 684
MbiI CCGCTC 1 cut(s) 364
MboI GATC 4 cut(s) 35, 237, 591, 682
MboII GAAGA 2 cut(s) 544, 596
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 4 cut(s) 342, 574, 765, 772
MluCI AATT 1 cut(s) 77
Mly113I GGCGCC 1 cut(s) 650
MlyI GAGTC 1 cut(s) 68
MroI TCCGGA 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 603
MseI TTAA 3 cut(s) 171, 471, 521
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 5 cut(s) 158, 289, 317, 697, 756
MspCI CTTAAG 2 cut(s) 470, 520
MspI CCGG 7 cut(s) 68, 183, 255, 380, 395, 680, 725
MspR9I CCNGG 2 cut(s) 255, 380
MvnI CGCG 2 cut(s) 279, 317
MwoI GCNNNNNNNGC 4 cut(s) 656, 665, 676, 713
NarI GGCGCC 1 cut(s) 650
NciI CCSGG 2 cut(s) 255, 380
NdeII GATC 4 cut(s) 35, 237, 591, 682
NlaIII CATG 7 cut(s) 232, 357, 370, 451, 591, 659, 704
NlaIV GGNNCC 6 cut(s) 37, 313, 359, 360, 465, 651
NspI RCATGY 2 cut(s) 659, 704
PaeI GCATGC 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 1 cut(s) 64
PflFI GACNNNGTC 1 cut(s) 101
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 7 cut(s) 188, 288, 291, 506, 654, 699, 718
PleI GAGTC 1 cut(s) 67
PluTI GGCGCC 1 cut(s) 653
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 1 cut(s) 358
PshAI GACNNNNGTC 1 cut(s) 158
Psp5II RGGWCCY 1 cut(s) 358
PspFI CCCAGC 1 cut(s) 756
PspN4I GGNNCC 6 cut(s) 37, 313, 359, 360, 465, 651
PspPI GGNCC 3 cut(s) 311, 358, 722
PspPPI RGGWCCY 1 cut(s) 358
PsuI RGATCY 1 cut(s) 35
PsyI GACNNNGTC 1 cut(s) 101
PvuII CAGCTG 2 cut(s) 289, 756
RsaI GTAC 5 cut(s) 233, 245, 377, 393, 620
RsaNI GTAC 5 cut(s) 232, 244, 376, 392, 619
RseI CAYNNNNRTG 1 cut(s) 371
SacII CCGCGG 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 3 cut(s) 171, 471, 521
SatI GCNGC 7 cut(s) 187, 287, 290, 505, 653, 698, 717
Sau3AI GATC 4 cut(s) 35, 237, 591, 682
Sau96I GGNCC 3 cut(s) 311, 358, 722
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 255, 380
SduI GDGCHC 4 cut(s) 342, 574, 765, 772
SfaNI GCATC 2 cut(s) 60, 718
SfoI GGCGCC 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 575
Sfr303I CCGCGG 1 cut(s) 318
SgrBI CCGCGG 1 cut(s) 318
SinI GGWCC 2 cut(s) 358, 722
SlaI CTCGAG 1 cut(s) 575
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 3 cut(s) 470, 520, 575
SmoI CTYRAG 3 cut(s) 470, 520, 575
SphI GCATGC 1 cut(s) 659
Sse9I AATT 1 cut(s) 77
SspDI GGCGCC 1 cut(s) 649
StyD4I CCNGG 2 cut(s) 253, 378
StyI CCWWGG 2 cut(s) 566, 674
TaaI ACNGT 3 cut(s) 103, 169, 329
TaiI ACGT 3 cut(s) 20, 299, 393
TaqI TCGA 4 cut(s) 54, 62, 342, 576
TasI AATT 1 cut(s) 77
TauI GCSGC 2 cut(s) 189, 655
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 3 cut(s) 171, 471, 521
Tru9I TTAA 3 cut(s) 171, 471, 521
TscAI CASTG 1 cut(s) 152
TseI GCWGC 5 cut(s) 286, 289, 504, 697, 716
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 1 cut(s) 152
Tth111I GACNNNGTC 1 cut(s) 101
Vha464I CTTAAG 2 cut(s) 470, 520
VpaK11BI GGWCC 2 cut(s) 358, 722
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 1 cut(s) 77
XceI RCATGY 2 cut(s) 659, 704
XhoI CTCGAG 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.