pycom15g30030

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005435
Physical Location & Seq
Forward (+)
27287002 .. 27287847
846 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g30030.1

Sequence Viewer

Length: 846 bp
ATGTCTGGTAATTCAATTCTCAAAGGAAAAGGAAAAACCATTGTTATGGCAGTGAAAGACACCTTCTACCTGTCCCACGGATCGCCGACGCTGAGCATCGACGAGTCGCTTCCGGCCAGAAAATTCCTCCAGTCGTGGAAAGAAACGGTGTATTCTCAGAAACCCACCTGCATCCTCATCATCTCCGGCCACTGGGACACCGCTGTCCCCTCCGTCAGCTCCATTTCCGGCCGCTACAGCACCATCTACGACTTCTACGGCTTCCCCAGATCCATGTACCAGATCAAGTACCCCGCCCCCGGCTCCCCCCACCTCGCCAACCGCGTCAAGCAGCTGCTGACCTCGTCGGGCTTTGGACGCGTCGACGTGGACACCAAGCGGGGGCTGGACCACGGCTCCTGGGTCCCGCTCATGTTGATGTACCCGGAGGCAGACATCCCAGTTTGCCAGCTGTCGGTCCAGTCGGGGAGGGACGCCACTTATCACTACAACTTGGGGAAGGCGCTGGCCCCACTGAGGGACGAGGGTGTTCTTATAGTGGCGTCAGGCAGCGCCACCCACAACTTGGGGGCGATGAGGAAGACCAAGAACAATGAGGTCTTCCCTTGGGCTCGCGAGTTTGACACGTGGCTCAAGAGGGCTCTTCTGGAAGGAAGGTACGAGGATGTGAACCAGTGGGAGAAGAAGGCGCCGCATGCGAAAACGGCGCACCCTTGGCCGGACCACCTTTACCCGTTGCATATCGTGATGGGGGCTGCTGGTGCGGACGTAAAGGCTAAGCAAATCCACGACAGCTGGGACCTCGGTGCTGTTTCGTACGCAGCCTACCAGTTTACATCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

282

Amino Acids

31.27

Weight (kDa)

9.12

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 21 - 278 4.5e-53 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 176
AasI GACNNNNNNGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 565
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 363
AccII CGCG 3 cut(s) 324, 360, 615
AciI CCGC 8 cut(s) 201, 232, 294, 322, 379, 407, 692, 764
AclWI GGATC 2 cut(s) 88, 264
AcoI YGGCCR 4 cut(s) 114, 187, 229, 716
AcsI RAATTY 1 cut(s) 122
AcvI CACGTG 1 cut(s) 627
AcyI GRCGYC 3 cut(s) 474, 542, 689
AfaI GTAC 5 cut(s) 278, 290, 422, 659, 818
AfiI CCNNNNNNNGG 8 cut(s) 192, 299, 381, 454, 516, 517, 565, 718
AflIII ACRYGT 2 cut(s) 358, 624
AgsI TTSAA 1 cut(s) 15
AjiI CACGTC 1 cut(s) 367
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 4 cut(s) 219, 334, 451, 795
AluI AGCT 4 cut(s) 219, 334, 451, 795
AlwI GGATC 2 cut(s) 88, 264
AlwNI CAGNNNCTG 1 cut(s) 337
AoxI GGCC 5 cut(s) 114, 187, 229, 507, 716
ApeKI GCWGC 5 cut(s) 331, 334, 549, 755, 821
ApoI RAATTY 1 cut(s) 122
AspLEI GCGC 4 cut(s) 505, 554, 691, 709
AspS9I GGNCC 6 cut(s) 388, 403, 457, 508, 721, 799
AsuC2I CCSGG 2 cut(s) 300, 425
AsuHPI GGTGA 1 cut(s) 831
AvaII GGWCC 5 cut(s) 388, 403, 457, 721, 799
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 2 cut(s) 613, 643
BbrPI CACGTG 1 cut(s) 627
BbsI GAAGAC 2 cut(s) 587, 592
BbvI GCAGC 5 cut(s) 321, 343, 561, 742, 833
BccI CCATC 2 cut(s) 251, 742
BceAI ACGGC 3 cut(s) 274, 409, 720
BciT130I CCWGG 1 cut(s) 400
BcnI CCSGG 2 cut(s) 300, 425
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 3 cut(s) 506, 555, 692
BfuAI ACCTGC 1 cut(s) 176
BisI GCNGC 7 cut(s) 232, 332, 335, 550, 692, 756, 822
BlpI GCTNAGC 2 cut(s) 92, 777
BlsI GCNGC 7 cut(s) 233, 333, 336, 551, 693, 757, 823
Bme1390I CCNGG 3 cut(s) 300, 400, 425
Bme18I GGWCC 5 cut(s) 388, 403, 457, 721, 799
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 6 cut(s) 388, 403, 457, 508, 721, 799
BmiI GGNNCC 7 cut(s) 304, 397, 404, 405, 510, 690, 800
BmrFI CCNGG 3 cut(s) 300, 400, 425
BmrI ACTGGG 2 cut(s) 202, 434
BmsI GCATC 2 cut(s) 105, 180
BmuI ACTGGG 2 cut(s) 202, 434
BpiI GAAGAC 2 cut(s) 587, 592
BpmI CTGGAG 1 cut(s) 113
Bpu1102I GCTNAGC 2 cut(s) 92, 777
BpuEI CTTGAG 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 300, 425
BsaAI YACGTR 1 cut(s) 627
BsaHI GRCGYC 3 cut(s) 474, 542, 689
BsaJI CCNNGG 7 cut(s) 76, 298, 391, 399, 605, 713, 802
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 8 cut(s) 192, 299, 381, 454, 516, 517, 565, 718
Bse1I ACTGG 6 cut(s) 130, 197, 440, 460, 673, 829
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 7 cut(s) 76, 298, 391, 399, 605, 713, 802
BseGI GGATG 3 cut(s) 171, 435, 670
BseLI CCNNNNNNNGG 8 cut(s) 192, 299, 381, 454, 516, 517, 565, 718
BseMII CTCAG 3 cut(s) 83, 170, 506
BseNI ACTGG 6 cut(s) 130, 197, 440, 460, 673, 829
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 5 cut(s) 321, 343, 561, 742, 833
BseYI CCCAGC 1 cut(s) 795
Bsh1236I CGCG 3 cut(s) 324, 360, 615
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 5 cut(s) 116, 189, 231, 509, 718
BshNI GGYRCC 1 cut(s) 688
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 6 cut(s) 113, 186, 228, 300, 425, 719
BsiWI CGTACG 1 cut(s) 816
BslFI GGGAC 7 cut(s) 58, 191, 209, 389, 485, 533, 812
BslI CCNNNNNNNGG 8 cut(s) 192, 299, 381, 454, 516, 517, 565, 718
BsmFI GGGAC 7 cut(s) 58, 191, 209, 389, 485, 533, 812
BsnI GGCC 5 cut(s) 116, 189, 231, 509, 718
Bsp1286I GDGCHC 2 cut(s) 613, 643
Bsp143I GATC 3 cut(s) 80, 269, 282
Bsp1720I GCTNAGC 2 cut(s) 92, 777
Bsp68I TCGCGA 1 cut(s) 615
BspACI CCGC 8 cut(s) 201, 232, 294, 322, 379, 407, 692, 764
BspANI GGCC 5 cut(s) 116, 189, 231, 509, 718
BspCNI CTCAG 3 cut(s) 84, 169, 507
BspFNI CGCG 3 cut(s) 324, 360, 615
BspLI GGNNCC 7 cut(s) 304, 397, 404, 405, 510, 690, 800
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 2 cut(s) 88, 264
BspQI GCTCTTC 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 409
BsrI ACTGG 6 cut(s) 130, 197, 440, 460, 673, 829
BssECI CCNNGG 7 cut(s) 76, 298, 391, 399, 605, 713, 802
BssMI GATC 3 cut(s) 80, 269, 282
BssNI GRCGYC 3 cut(s) 474, 542, 689
BssT1I CCWWGG 2 cut(s) 605, 713
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 648
BstACI GRCGYC 3 cut(s) 474, 542, 689
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 4 cut(s) 449, 507, 613, 696
BstDEI CTNAG 4 cut(s) 92, 156, 515, 777
BstDSI CCRYGG 2 cut(s) 76, 391
BstF5I GGATG 3 cut(s) 171, 435, 670
BstFNI CGCG 3 cut(s) 324, 360, 615
BstH2I RGCGCY 3 cut(s) 506, 555, 692
BstHHI GCGC 4 cut(s) 505, 554, 691, 709
BstKTI GATC 3 cut(s) 83, 272, 285
BstMBI GATC 3 cut(s) 80, 269, 282
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 6 cut(s) 237, 357, 695, 704, 715, 761
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 698
BstSCI CCNGG 3 cut(s) 298, 398, 423
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 3 cut(s) 324, 360, 615
BstV1I GCAGC 5 cut(s) 321, 343, 561, 742, 833
BstV2I GAAGAC 2 cut(s) 587, 592
BstX2I RGATCY 1 cut(s) 269
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 269
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 5 cut(s) 116, 189, 231, 509, 718
BtgI CCRYGG 2 cut(s) 76, 391
BtgZI GCGATG 1 cut(s) 587
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 3 cut(s) 171, 435, 670
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 4 cut(s) 57, 190, 512, 680
BtuMI TCGCGA 1 cut(s) 615
BveI ACCTGC 1 cut(s) 176
Cac8I GCNNGC 4 cut(s) 449, 507, 613, 696
CaiI CAGNNNCTG 1 cut(s) 337
CfoI GCGC 4 cut(s) 505, 554, 691, 709
Cfr13I GGNCC 6 cut(s) 388, 403, 457, 508, 721, 799
CseI GACGC 6 cut(s) 97, 313, 349, 366, 482, 531
Csp6I GTAC 5 cut(s) 277, 289, 421, 658, 817
CviAII CATG 3 cut(s) 274, 412, 695
CviQI GTAC 5 cut(s) 277, 289, 421, 658, 817
DdeI CTNAG 4 cut(s) 92, 156, 515, 777
DinI GGCGCC 1 cut(s) 690
DpnI GATC 3 cut(s) 82, 271, 284
DpnII GATC 3 cut(s) 80, 269, 282
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 4 cut(s) 114, 187, 229, 716
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 605, 713
Eco24I GRGCYC 2 cut(s) 613, 643
Eco47I GGWCC 5 cut(s) 388, 403, 457, 721, 799
Eco52I CGGCCG 1 cut(s) 229
Eco72I CACGTG 1 cut(s) 627
EcoO109I RGGNCCY 2 cut(s) 403, 799
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 2 cut(s) 605, 713
EcoT38I GRGCYC 2 cut(s) 613, 643
EgeI GGCGCC 1 cut(s) 690
EheI GGCGCC 1 cut(s) 690
ErhI CCWWGG 2 cut(s) 605, 713
FaeI CATG 3 cut(s) 277, 415, 698
FaiI YATR 6 cut(s) 47, 275, 413, 536, 696, 741
FaqI GGGAC 7 cut(s) 58, 191, 209, 389, 485, 533, 812
FatI CATG 3 cut(s) 273, 411, 694
FauI CCCGC 3 cut(s) 301, 372, 414
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 7 cut(s) 232, 332, 335, 550, 692, 756, 822
FokI GGATG 3 cut(s) 158, 422, 677
FriOI GRGCYC 2 cut(s) 613, 643
Fsp4HI GCNGC 7 cut(s) 232, 332, 335, 550, 692, 756, 822
GlaI GCGC 4 cut(s) 504, 553, 690, 708
GluI GCNGC 7 cut(s) 232, 332, 335, 550, 692, 756, 822
GsaI CCCAGC 1 cut(s) 799
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 3 cut(s) 506, 555, 692
HaeIII GGCC 5 cut(s) 116, 189, 231, 509, 718
HapII CCGG 6 cut(s) 113, 186, 228, 300, 425, 719
HgaI GACGC 6 cut(s) 97, 313, 349, 366, 482, 531
HhaI GCGC 4 cut(s) 505, 554, 691, 709
Hin1I GRCGYC 3 cut(s) 474, 542, 689
Hin1II CATG 3 cut(s) 277, 415, 698
Hin6I GCGC 4 cut(s) 503, 552, 689, 707
HinP1I GCGC 4 cut(s) 503, 552, 689, 707
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 104
HpaII CCGG 6 cut(s) 113, 186, 228, 300, 425, 719
HphI GGTGA 1 cut(s) 831
Hpy166II GTNNAC 4 cut(s) 364, 370, 670, 834
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 4 cut(s) 614, 634, 647, 745
Hpy8I GTNNAC 4 cut(s) 364, 370, 670, 834
Hpy99I CGWCG 5 cut(s) 91, 104, 349, 365, 368
HpyAV CCTTC 5 cut(s) 73, 493, 644, 648, 679
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 3 cut(s) 366, 626, 768
HpyCH4V TGCA 2 cut(s) 171, 739
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 357, 695, 704, 715, 761
HpyF3I CTNAG 4 cut(s) 92, 156, 515, 777
HpySE526I ACGT 3 cut(s) 366, 626, 768
Hsp92I GRCGYC 3 cut(s) 474, 542, 689
Hsp92II CATG 3 cut(s) 277, 415, 698
HspAI GCGC 4 cut(s) 503, 552, 689, 707
KasI GGCGCC 1 cut(s) 688
KflI GGGWCCC 1 cut(s) 403
Kzo9I GATC 3 cut(s) 80, 269, 282
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 3 cut(s) 224, 308, 401
Lsp1109I GCAGC 5 cut(s) 321, 343, 561, 742, 833
LweI GCATC 2 cut(s) 105, 180
MaeII ACGT 3 cut(s) 366, 626, 768
MalI GATC 3 cut(s) 82, 271, 284
MbiI CCGCTC 1 cut(s) 409
MboI GATC 3 cut(s) 80, 269, 282
MboII GAAGA 4 cut(s) 592, 592, 635, 694
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 2 cut(s) 613, 643
MluCI AATT 3 cut(s) 10, 15, 122
MluI ACGCGT 1 cut(s) 358
Mly113I GGCGCC 1 cut(s) 689
MlyI GAGTC 1 cut(s) 113
MslI CAYNNNNRTG 2 cut(s) 44, 416
MspA1I CMGCKG 4 cut(s) 203, 334, 451, 795
MspI CCGG 6 cut(s) 113, 186, 228, 300, 425, 719
MspR9I CCNGG 3 cut(s) 300, 400, 425
MvaI CCWGG 1 cut(s) 400
MvnI CGCG 3 cut(s) 324, 360, 615
MwoI GCNNNNNNNGC 6 cut(s) 237, 357, 695, 704, 715, 761
NarI GGCGCC 1 cut(s) 689
NciI CCSGG 2 cut(s) 300, 425
NdeII GATC 3 cut(s) 80, 269, 282
NlaIII CATG 3 cut(s) 277, 415, 698
NlaIV GGNNCC 7 cut(s) 304, 397, 404, 405, 510, 690, 800
NruI TCGCGA 1 cut(s) 615
NspI RCATGY 1 cut(s) 698
PaeI GCATGC 1 cut(s) 698
PaqCI CACCTGC 1 cut(s) 176
PciSI GCTCTTC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 321
Pfl23II CGTACG 1 cut(s) 816
PflFI GACNNNGTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 565
PkrI GCNGC 7 cut(s) 233, 333, 336, 551, 693, 757, 823
PleI GAGTC 1 cut(s) 112
PluTI GGCGCC 1 cut(s) 692
PmaCI CACGTG 1 cut(s) 627
PmlI CACGTG 1 cut(s) 627
PpsI GAGTC 1 cut(s) 112
Ppu21I YACGTR 1 cut(s) 627
PpuMI RGGWCCY 2 cut(s) 403, 799
Psp5II RGGWCCY 2 cut(s) 403, 799
Psp6I CCWGG 1 cut(s) 398
PspCI CACGTG 1 cut(s) 627
PspFI CCCAGC 1 cut(s) 795
PspGI CCWGG 1 cut(s) 398
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 7 cut(s) 304, 397, 404, 405, 510, 690, 800
PspPI GGNCC 6 cut(s) 388, 403, 457, 508, 721, 799
PspPPI RGGWCCY 2 cut(s) 403, 799
PstNI CAGNNNCTG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 269
PsyI GACNNNGTC 1 cut(s) 343
PvuII CAGCTG 3 cut(s) 334, 451, 795
RruI TCGCGA 1 cut(s) 615
RsaI GTAC 5 cut(s) 278, 290, 422, 659, 818
RsaNI GTAC 5 cut(s) 277, 289, 421, 658, 817
RseI CAYNNNNRTG 2 cut(s) 44, 416
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 648
SatI GCNGC 7 cut(s) 232, 332, 335, 550, 692, 756, 822
Sau3AI GATC 3 cut(s) 80, 269, 282
Sau96I GGNCC 6 cut(s) 388, 403, 457, 508, 721, 799
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 3 cut(s) 300, 400, 425
SduI GDGCHC 2 cut(s) 613, 643
SfaNI GCATC 2 cut(s) 105, 180
SfcI CTRYAG 1 cut(s) 235
SfoI GGCGCC 1 cut(s) 690
SgrDI CGTCGACG 1 cut(s) 362
SinI GGWCC 5 cut(s) 388, 403, 457, 721, 799
SmiMI CAYNNNNRTG 2 cut(s) 44, 416
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
SphI GCATGC 1 cut(s) 698
Sse9I AATT 3 cut(s) 10, 15, 122
SsiI CCGC 8 cut(s) 201, 232, 294, 322, 379, 407, 692, 764
SspDI GGCGCC 1 cut(s) 688
StyD4I CCNGG 3 cut(s) 298, 398, 423
StyI CCWWGG 2 cut(s) 605, 713
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 3 cut(s) 369, 629, 771
TaqI TCGA 2 cut(s) 99, 363
TaqII GACCGA 1 cut(s) 445
TasI AATT 3 cut(s) 10, 15, 122
TauI GCSGC 2 cut(s) 234, 694
TscAI CASTG 4 cut(s) 57, 197, 519, 680
TseI GCWGC 5 cut(s) 331, 334, 549, 755, 821
TspGWI ACGGA 2 cut(s) 93, 202
TspRI CASTG 4 cut(s) 57, 197, 519, 680
Tth111I GACNNNGTC 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 5 cut(s) 388, 403, 457, 721, 799
XapI RAATTY 1 cut(s) 122
XceI RCATGY 1 cut(s) 698
XcmI CCANNNNNNNNNTGG 2 cut(s) 382, 562
XmiI GTMKAC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.