AT4G18330

Eukaryotic translation initiation factor 2 subunit gamma-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002084
Physical Location & Seq
Forward (+)
10126533 .. 10128734
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18330.1

Sequence Viewer

Length: 855 bp
ATGGTTCAATCTGTGTTAATTTCGCGTAACAAGGGTTTGGCGGAGCAAGATCTAACTAAGCTTGATGTAACAAAGTTACATCCTCTGTTTCCTGAAGTCATTTCTCGCCAAGCAACCATAAATATCGGAACCATTGGTCATGTCGCTCACGGTAAATCGACTATAGTGAAAGCTATCTCTGGTGTTCAGACTGTGCGTTTTAAGAATGAATTAGAGCGTAACATTACCATTAAGCTTGGTTATGCAAATGCAAAGATTTACAAGTGTGAGGATGACAAGTGCCCTAGACCAATGTGCTACAAGTCATATGGGAGTGGAAAAGAAGACAACCCAACTTGTGATGTCCCCGGATTTGAGAATTGCAAGATGACACTACGGAGACATGTCTCATGTGTCGATTGTCCGGGACATGATATTCTTATGGCGACAATGCTCAATGGAGCAGCAATCGTGGATGGTGCTTTACTTCTAATTGCTGCTAACGAGAGTTGTCCACAACCACAAACCGCTGAACATCTTGCTTCTGTTGATATGATGCGCCTTAAGCATATCATAATCCTTCAGAACAAGATTGATCTCATTAATGAGAAAGCCGCCACTGAACAGCACGAGGCTATTCAGAAATTTATAACGAACACGAATGCTGAGGATGCACCTATAGTTCCTGTCTCAGCACAACTGAAATACAACATTGATGTTGTGAGTGAATACATTGTCAAGAAGATTCCAATCCCTGTGAGAGACTTTGTGTCACCACCAAAGATGATTGTGATTCGTTCTTTCGATGTCAATAAACCTGGCTCGGCGGGTCATGAAATGAAAGGTGGTGTTGCTGGTGGAAGTATCCTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 eIF2_C

Protein Analysis

284

Amino Acids

31.08

Weight (kDa)

8.35

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 39 - 243 1.8e-27 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AccII CGCG 1 cut(s) 25
AciI CCGC 4 cut(s) 41, 507, 594, 806
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 114, 545
AflII CTTAAG 1 cut(s) 542
AflIII ACRYGT 1 cut(s) 382
AgsI TTSAA 1 cut(s) 8
AhdI GACNNNNNGTC 1 cut(s) 748
AjnI CCWGG 1 cut(s) 796
AluBI AGCT 3 cut(s) 61, 173, 235
AluI AGCT 3 cut(s) 61, 173, 235
Alw26I GTCTC 4 cut(s) 373, 391, 673, 735
ApeKI GCWGC 2 cut(s) 443, 476
ApoI RAATTY 1 cut(s) 623
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 540
AsuC2I CCSGG 2 cut(s) 348, 405
AsuHPI GGTGA 1 cut(s) 744
BaeGI GKGCMC 1 cut(s) 284
BauI CACGAG 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 645
BbvI GCAGC 2 cut(s) 455, 463
BccI CCATC 1 cut(s) 449
BciT130I CCWGG 1 cut(s) 798
BciVI GTATCC 1 cut(s) 854
BcnI CCSGG 2 cut(s) 348, 405
BcoDI GTCTC 4 cut(s) 373, 391, 673, 735
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 2 cut(s) 162, 657
BfrI CTTAAG 1 cut(s) 542
BfuI GTATCC 1 cut(s) 854
BglII AGATCT 1 cut(s) 49
BisI GCNGC 3 cut(s) 444, 477, 594
BlsI GCNGC 3 cut(s) 445, 478, 595
Bme1390I CCNGG 3 cut(s) 348, 405, 798
BmeRI GACNNNNNGTC 1 cut(s) 748
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 3 cut(s) 348, 405, 798
BmsI GCATC 2 cut(s) 525, 640
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 645
BpuMI CCSGG 2 cut(s) 348, 405
BsaBI GATNNNNATC 1 cut(s) 728
BsaJI CCNNGG 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 850
Bse8I GATNNNNATC 1 cut(s) 728
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 4 cut(s) 79, 277, 460, 655
BseJI GATNNNNATC 1 cut(s) 728
BseMII CTCAG 2 cut(s) 636, 684
BseNI ACTGG 1 cut(s) 850
BseSI GKGCMC 1 cut(s) 284
BseXI GCAGC 2 cut(s) 455, 463
Bsh1236I CGCG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 348, 404
BslFI GGGAC 2 cut(s) 329, 420
BsmAI GTCTC 4 cut(s) 373, 391, 673, 735
BsmFI GGGAC 2 cut(s) 329, 420
BsmI GAATGC 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 2 cut(s) 49, 574
BspACI CCGC 4 cut(s) 41, 507, 594, 806
BspCNI CTCAG 2 cut(s) 637, 683
BspFNI CGCG 1 cut(s) 25
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 1 cut(s) 130
BspTI CTTAAG 1 cut(s) 542
BsrI ACTGG 1 cut(s) 850
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 2 cut(s) 49, 574
BssSI CACGAG 1 cut(s) 608
Bst2BI CACGAG 1 cut(s) 608
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 2 cut(s) 152, 193
BstAFI CTTAAG 1 cut(s) 542
BstDEI CTNAG 3 cut(s) 57, 645, 670
BstF5I GGATG 4 cut(s) 79, 277, 460, 655
BstFNI CGCG 1 cut(s) 25
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 2 cut(s) 52, 577
BstMAI GTCTC 4 cut(s) 373, 391, 673, 735
BstMBI GATC 2 cut(s) 49, 574
BstMWI GCNNNNNNNGC 2 cut(s) 544, 650
BstNI CCWGG 1 cut(s) 798
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 346, 403, 796
BstSFI CTRYAG 2 cut(s) 162, 657
BstSLI GKGCMC 1 cut(s) 284
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 455, 463
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuI GTATCC 1 cut(s) 854
BtsCI GGATG 4 cut(s) 79, 277, 460, 655
BtsIMutI CAGTG 1 cut(s) 597
CciI TCATGA 1 cut(s) 811
CfoI GCGC 1 cut(s) 540
CviAII CATG 5 cut(s) 140, 383, 390, 410, 812
CviJI RGCY 6 cut(s) 61, 173, 235, 593, 614, 801
CviKI_1 RGCY 6 cut(s) 61, 173, 235, 593, 614, 801
DdeI CTNAG 3 cut(s) 57, 645, 670
DpnI GATC 2 cut(s) 51, 576
DpnII GATC 2 cut(s) 49, 574
DriI GACNNNNNGTC 1 cut(s) 748
Eam1105I GACNNNNNGTC 1 cut(s) 748
EciI GGCGGA 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 114, 545
EcoRII CCWGG 1 cut(s) 796
FaeI CATG 5 cut(s) 143, 386, 393, 413, 815
FaqI GGGAC 2 cut(s) 329, 420
FatI CATG 5 cut(s) 139, 382, 389, 409, 811
FauI CCCGC 1 cut(s) 799
FauNDI CATATG 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 444, 477, 594
FokI GGATG 4 cut(s) 66, 284, 467, 662
Fsp4HI GCNGC 3 cut(s) 444, 477, 594
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 539
GluI GCNGC 3 cut(s) 444, 477, 594
GsuI CTGGAG 1 cut(s) 833
HapII CCGG 2 cut(s) 348, 404
HhaI GCGC 1 cut(s) 540
Hin1II CATG 5 cut(s) 143, 386, 393, 413, 815
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HindIII AAGCTT 2 cut(s) 59, 233
HinfI GANTC 2 cut(s) 724, 772
HpaII CCGG 2 cut(s) 348, 404
HphI GGTGA 1 cut(s) 744
Hpy166II GTNNAC 1 cut(s) 494
Hpy188I TCNGA 4 cut(s) 128, 189, 564, 621
Hpy188III TCNNGA 3 cut(s) 92, 718, 812
Hpy8I GTNNAC 1 cut(s) 494
HpyAV CCTTC 1 cut(s) 569
HpyCH4III ACNGT 2 cut(s) 152, 193
HpyCH4V TGCA 4 cut(s) 245, 251, 363, 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 544, 650
HpyF3I CTNAG 3 cut(s) 57, 645, 670
Hsp92II CATG 5 cut(s) 143, 386, 393, 413, 815
HspAI GCGC 1 cut(s) 538
Kzo9I GATC 2 cut(s) 49, 574
LmnI GCTCC 2 cut(s) 43, 440
LpnPI CCDG 9 cut(s) 105, 165, 361, 417, 678, 747, 783, 810, 819
Lsp1109I GCAGC 2 cut(s) 455, 463
LweI GCATC 2 cut(s) 525, 640
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 5 cut(s) 26, 67, 75, 218, 750
MalI GATC 2 cut(s) 51, 576
MboI GATC 2 cut(s) 49, 574
MboII GAAGA 2 cut(s) 335, 733
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 5 cut(s) 18, 209, 358, 471, 623
MnlI CCTC 4 cut(s) 93, 262, 604, 640
MseI TTAA 5 cut(s) 17, 201, 231, 543, 582
MspA1I CMGCKG 1 cut(s) 509
MspCI CTTAAG 1 cut(s) 542
MspI CCGG 2 cut(s) 348, 404
MspR9I CCNGG 3 cut(s) 348, 405, 798
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 2 cut(s) 544, 650
NciI CCSGG 2 cut(s) 348, 405
NdeI CATATG 1 cut(s) 307
NdeII GATC 2 cut(s) 49, 574
NlaIII CATG 5 cut(s) 143, 386, 393, 413, 815
NlaIV GGNNCC 1 cut(s) 130
NmeAIII GCCGAG 1 cut(s) 782
NmuCI GTSAC 1 cut(s) 750
NspI RCATGY 1 cut(s) 386
PagI TCATGA 1 cut(s) 811
PciI ACATGT 1 cut(s) 382
PctI GAATGC 1 cut(s) 646
PfeI GAWTC 2 cut(s) 724, 772
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 3 cut(s) 445, 478, 595
PscI ACATGT 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 796
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 1 cut(s) 130
PsuI RGATCY 1 cut(s) 49
SaqAI TTAA 5 cut(s) 17, 201, 231, 543, 582
SatI GCNGC 3 cut(s) 444, 477, 594
Sau3AI GATC 2 cut(s) 49, 574
ScrFI CCNGG 3 cut(s) 348, 405, 798
SduI GDGCHC 1 cut(s) 284
SetI ASST 6 cut(s) 63, 175, 237, 658, 799, 826
SfaNI GCATC 2 cut(s) 525, 640
SfcI CTRYAG 2 cut(s) 162, 657
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 5 cut(s) 18, 209, 358, 471, 623
SsiI CCGC 4 cut(s) 41, 507, 594, 806
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 3 cut(s) 346, 403, 796
TaaI ACNGT 2 cut(s) 152, 193
TaqI TCGA 3 cut(s) 158, 396, 783
TasI AATT 5 cut(s) 18, 209, 358, 471, 623
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 2 cut(s) 724, 772
Tru1I TTAA 5 cut(s) 17, 201, 231, 543, 582
Tru9I TTAA 5 cut(s) 17, 201, 231, 543, 582
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 1 cut(s) 750
TseI GCWGC 2 cut(s) 443, 476
Tsp45I GTSAC 1 cut(s) 750
TspDTI ATGAA 3 cut(s) 222, 828, 833
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 1 cut(s) 604
Vha464I CTTAAG 1 cut(s) 542
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 386
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.