Rorug04G0182500

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000325
Physical Location & Seq
Forward (+)
31923248 .. 31928699
5452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0182500.1

Sequence Viewer

Length: 1551 bp
ATGGAGAGTGTGGTGAACTTCCCGTATAGCCATTGCAAGATAGCCCATACATGGAGCTTCAAACACAGCACCCTCCTCTTCCCCAAAACCCAAACCGCAAAACCCTCCACCAAAACCAAAACCAAATCCCTCCCCTCTACTCTTTCTCGTTGCCATGCCCAGTTCTTCTCACCACCGCATAACACCAGTTCCCCAAAGCACACCACCCTCCATTCCCATGAATACCACAAGCTCAAAGCCTTACTGGAGATTCTCATGGAGAAGGAGTGTTGCCCTTTGCAAGTGCTTAGAGATGATGGTGATTGGACCAGAGACCAATTCTGGGCTGTCATCAGATTCCTCATACATGCCTCCAGGCCCAAGGAAATCTTGCAGTTGTTTGATGTATGGAGGAACATTGAGAAATCGCGGATTAATGAATTCAACTACAGCAAGATAATAGGATTGTTGGTTGAGGAGGACCTGATTGAAGAGGCAGTGCTGTGCTTTCAGGATATGAAGAGTCATGGTCTTAGGCTGTCTGTGGAAGTATACAATTCAATTATTCATGGTCTTTCGAGAAAAGGAAACTTTGATGATGCTGAACTCTTCCTTAACGAGATGAAAGAAATGAATTTGGCACCAGACACTGATACTTATGATGGGCTCATTGAGGCTTATGGAAAATATAAAATGTATGATGAGATGGGTATGTGTCTGAAGAAAATGAGATTAAATGGCTGTTCACCAGACCAGATTACCTATAATTTGCTTATTCGAGAGTTTGCACGTGGAGGGTTACTGAAAAGGATGGAAAGAGTATATCAATCCATGGTTTCAAAAAGAATGGATTTGCAGGCTCCTACTTTGATTGCAATGCTGGAAGTTTATGCAAAATTTGGGATCTTGGAGAAGATGGAAGTGTTTTATAGAAGAGTTTTGAACTCGAGAGCAATTTTAAAGGAGGATTTGATTAAGAAAGTTGCAGAAGTTTATATTGAGAACTATATGTTTTCCAGATTAGAGAACTTGGGAGTTGATCTTTCACCAAGATTTGGTCAGACTGACCTTGTTTGGTGTCTGCGTCTCCTTTCTCATGCTGGTCTTTTGAGTCGAAGAGGTATGGATTCCATCATTCTAGAGATGGAAGAAAAAGGTGTCCCATGGAATGCAACGGTGGCAAACATAATAATGCTAGCTTATTTGAAGATGAAAGATTTCACCCGCCTGAGAATCATGTTCTCCCAATCACTAACCCATGGTGTGGAGCCCGATATTATCACTGTTGGAATTCTATTTGATGCAAATAGGATTGGCTATGATGGGTCTGCGACTTTAGATGCATGGAGGAAGCAGGGTTTTCTTTACAAAGCAGTTGAAATGAACACCGATCCTCTCGTTATTACTACATTTGGGAAGGGGCATTTCCTCAGGAACTGTGAAGCAGCATACTCCTCTCTTGAACCTGAAGTTAGAGAAAAGAAAACATGGACTTACCAAGATCTCATTGATTCAGTGTTCAAAGACAATCAGTGTAACCTCAGTAAGAGGGACTTAATGAAGTCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 eIF2_C

Protein Analysis

516

Amino Acids

59.85

Weight (kDa)

8.49

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 124 - 278 1.1e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 163 - 222 1.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 170 - 202 5.9e-07 PPR repeat
PPR_2 PF13041 175 - 221 1e-11 PPR repeat family
PPR PF01535 177 - 205 1e-06 PPR repeat
PPR_2 PF13041 215 - 257 2.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24391 FvH4_2g38071 FvH4_3g07131 FvH4_3g22481 FvH4_4g15809 FvH4_5g37230 FvH4_7g25201 FvH4_7g29092 FvH4_7g29093
malus_domestica MD01G1197600.v1.1 MD17G1186900.v1.1
prunus_persica Prupe.1G227400_v2.0.a1 Prupe.2G291000_v2.0.a1 Prupe.4G268600_v2.0.a1 Prupe.5G094100_v2.0.a1
pyrus_communis pycom01g20780 pycom17g19620
rosa_chinensis RchiOBHm_Chr1g0376821 RchiOBHm_Chr5g0061941 RchiOBHm_Chr5g0078991 RchiOBHm_Chr6g0276821 RchiOBHm_Chr7g0191321
rosa_laevigata RLG00000007883 RLG00000008804 RLG00000010632 RLG00000026567 RLG00000035244 RLG00000036018 RLG00000036909
rosa_multiflora Rmu_sc0001036.1_g000026 Rmu_sc0001556.1_g000021 Rmu_sc0001974.1_g000022 Rmu_sc0002113.1_g000006 Rmu_sc0002572.1_g000008 Rmu_sc0004808.1_g000011 Rmu_sc0005117.1_g000017 Rmu_sc0005319.1_g000016 Rmu_sc0008432.1_g000005 Rmu_sc0010475.1_g000001 Rmu_sc0015522.1_g000003 Rmu_ssc0000066.1_g000009 Rmu_ssc0000400.1_g000102 Rmu_ssc0000400.1_g000103
rosa_roxburghii Rroxscaffold_1G00071290 Rroxscaffold_2G00141280 Rroxscaffold_3G00233790 Rroxscaffold_4G00297530 Rroxscaffold_5G00368860 Rroxscaffold_5G00386220 Rroxscaffold_7G00159400
rosa_rugosa Rorug01G0146800.1 Rorug01G0162100.1 Rorug01G0162200.1 Rorug01G0398600 Rorug01G0398700 Rorug02G0344200 Rorug02G0344300 Rorug02G0344400 Rorug03G0046200 Rorug03G0153200 Rorug04G0182500 Rorug04G0182600 Rorug05G0257700 Rorug05G0386400 Rorug05G0386400 Rorug05G0386500 Rorug05G0460900.1 Rorug05G0467000 Rorug07G0110400
rosa_samantha Rh1AG183500 Rh1AG416000 Rh1BG023600 Rh1BG145400 Rh1BG145500 Rh1BG278800 Rh1BG375400 Rh1CG389200 Rh1DG166300 Rh1DG176900 Rh1DG309900 Rh1DG406000 Rh2BG439100 Rh2BG502200 Rh2CG314800 Rh2CG517400 Rh2CG532000 Rh2DG449100 Rh3BG109900 Rh3CG230400 Rh3CG246600 Rh3CG289400 Rh3CG289500 Rh3DG120600 Rh4AG144400 Rh4AG408200 Rh4BG419300 Rh4CG101900 Rh4CG151000 Rh4CG226900 Rh4DG235400 Rh5AG265900 Rh5AG375200 Rh5CG559600 Rh5DG218200 Rh6AG074500 Rh6BG291800 Rh6CG292800 Rh6DG284900
rosa_wichuraiana Rw0G013240 Rw1G036490 Rw5G024920 Rw5G030730 Rw5G033710 Rw6G033840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 619
AccB7I CCANNNNNTGG 2 cut(s) 1034, 1243
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 409
AciI CCGC 4 cut(s) 96, 176, 409, 1204
AclWI GGATC 2 cut(s) 890, 1364
AcsI RAATTY 4 cut(s) 419, 613, 875, 1269
AcuI CTGAAG 2 cut(s) 719, 1467
AcvI CACGTG 1 cut(s) 770
AfiI CCNNNNNNNGG 4 cut(s) 51, 322, 1034, 1243
AjnI CCWGG 1 cut(s) 353
AluBI AGCT 3 cut(s) 57, 232, 1178
AluI AGCT 3 cut(s) 57, 232, 1178
Alw26I GTCTC 2 cut(s) 306, 1070
AlwI GGATC 2 cut(s) 890, 1364
AlwNI CAGNNNCTG 2 cut(s) 629, 1416
Ama87I CYCGRG 1 cut(s) 925
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 1424
ApoI RAATTY 4 cut(s) 419, 613, 875, 1269
AseI ATTAAT 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 1196
AspS9I GGNCC 3 cut(s) 306, 357, 460
AsuHPI GGTGA 6 cut(s) 25, 162, 311, 717, 1017, 1192
AsuNHI GCTAGC 1 cut(s) 1174
AvaI CYCGRG 1 cut(s) 925
AvaII GGWCC 2 cut(s) 306, 460
AxyI CCTNAGG 1 cut(s) 1409
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 2 cut(s) 648, 1251
BbrPI CACGTG 1 cut(s) 770
BbvI GCAGC 1 cut(s) 1436
BccI CCATC 8 cut(s) 290, 635, 679, 784, 889, 1117, 1118, 1295
BciT130I CCWGG 1 cut(s) 355
BcoDI GTCTC 2 cut(s) 306, 1070
BfaI CTAG 2 cut(s) 1118, 1175
BfmI CTRYAG 1 cut(s) 427
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 1 cut(s) 1425
BlsI GCNGC 1 cut(s) 1426
Bme1390I CCNGG 1 cut(s) 355
Bme18I GGWCC 2 cut(s) 306, 460
BmeT110I CYCGRG 1 cut(s) 925
BmgT120I GGNCC 3 cut(s) 306, 357, 460
BmiI GGNNCC 3 cut(s) 621, 840, 1248
BmrFI CCNGG 1 cut(s) 355
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 3 cut(s) 568, 1270, 1309
BmtI GCTAGC 1 cut(s) 1178
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 2 cut(s) 266, 337
BsaAI YACGTR 1 cut(s) 770
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 360, 810, 1142, 1237
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 322, 1034, 1243
Bse1I ACTGG 3 cut(s) 160, 186, 249
Bse21I CCTNAGG 1 cut(s) 1409
Bse3DI GCAATG 2 cut(s) 31, 861
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 4 cut(s) 360, 810, 1142, 1237
BseGI GGATG 1 cut(s) 795
BseLI CCNNNNNNNGG 4 cut(s) 51, 322, 1034, 1243
BseMI GCAATG 2 cut(s) 31, 861
BseMII CTCAG 3 cut(s) 1199, 1423, 1534
BseNI ACTGG 3 cut(s) 160, 186, 249
BseRI GAGGAG 3 cut(s) 65, 470, 1423
BseXI GCAGC 1 cut(s) 1436
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 1 cut(s) 358
BshNI GGYRCC 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 925
BslFI GGGAC 2 cut(s) 1124, 1544
BslI CCNNNNNNNGG 4 cut(s) 51, 322, 1034, 1243
BsmAI GTCTC 2 cut(s) 306, 1070
BsmBI CGTCTC 1 cut(s) 1070
BsmFI GGGAC 2 cut(s) 1124, 1544
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 1 cut(s) 358
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 925
Bsp1286I GDGCHC 2 cut(s) 648, 1251
Bsp143I GATC 4 cut(s) 882, 1018, 1369, 1480
Bsp19I CCATGG 3 cut(s) 810, 1142, 1237
BspACI CCGC 4 cut(s) 96, 176, 409, 1204
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 3 cut(s) 1200, 1422, 1533
BspFNI CGCG 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 621, 840, 1248
BspOI GCTAGC 1 cut(s) 1178
BspPI GGATC 2 cut(s) 890, 1364
BspT107I GGYRCC 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 2 cut(s) 31, 861
BsrI ACTGG 3 cut(s) 160, 186, 249
BssECI CCNNGG 4 cut(s) 360, 810, 1142, 1237
BssMI GATC 4 cut(s) 882, 1018, 1369, 1480
BssNAI GTATAC 1 cut(s) 532
BssT1I CCWWGG 4 cut(s) 360, 810, 1142, 1237
Bst1107I GTATAC 1 cut(s) 532
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 3 cut(s) 1156, 1264, 1418
Bst6I CTCTTC 6 cut(s) 83, 465, 494, 593, 907, 1090
BstBAI YACGTR 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 837, 1176
BstDEI CTNAG 5 cut(s) 287, 512, 1208, 1409, 1520
BstDSI CCRYGG 3 cut(s) 810, 1142, 1237
BstF5I GGATG 1 cut(s) 795
BstFNI CGCG 1 cut(s) 409
BstKTI GATC 4 cut(s) 885, 1021, 1372, 1483
BstMAI GTCTC 2 cut(s) 306, 1070
BstMBI GATC 4 cut(s) 882, 1018, 1369, 1480
BstMWI GCNNNNNNNGC 1 cut(s) 1157
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 1 cut(s) 427
BstUI CGCG 1 cut(s) 409
BstV1I GCAGC 1 cut(s) 1436
BstX2I RGATCY 2 cut(s) 882, 1480
BstYI RGATCY 2 cut(s) 882, 1480
BstZ17I GTATAC 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 1409
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 3 cut(s) 810, 1142, 1237
BtsCI GGATG 1 cut(s) 795
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 5 cut(s) 483, 627, 1260, 1500, 1517
Cac8I GCNNGC 2 cut(s) 837, 1176
CaiI CAGNNNCTG 2 cut(s) 629, 1416
Cfr13I GGNCC 3 cut(s) 306, 357, 460
CseI GACGC 1 cut(s) 1052
DdeI CTNAG 5 cut(s) 287, 512, 1208, 1409, 1520
DpnI GATC 4 cut(s) 884, 1020, 1371, 1482
DpnII GATC 4 cut(s) 882, 1018, 1369, 1480
DraI TTTAAA 1 cut(s) 939
Eam1104I CTCTTC 6 cut(s) 83, 465, 494, 593, 907, 1090
EarI CTCTTC 6 cut(s) 83, 465, 494, 593, 907, 1090
Eco130I CCWWGG 4 cut(s) 360, 810, 1142, 1237
Eco24I GRGCYC 2 cut(s) 648, 1251
Eco31I GGTCTC 1 cut(s) 306
Eco47I GGWCC 2 cut(s) 306, 460
Eco57I CTGAAG 2 cut(s) 719, 1467
Eco72I CACGTG 1 cut(s) 770
Eco81I CCTNAGG 1 cut(s) 1409
Eco88I CYCGRG 1 cut(s) 925
EcoO109I RGGNCCY 1 cut(s) 460
EcoRI GAATTC 2 cut(s) 419, 1269
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 4 cut(s) 360, 810, 1142, 1237
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 2 cut(s) 648, 1251
ErhI CCWWGG 4 cut(s) 360, 810, 1142, 1237
Esp3I CGTCTC 1 cut(s) 1070
FalI AAGNNNNNCTT 4 cut(s) 353, 385, 1517, 1549
FaqI GGGAC 2 cut(s) 1124, 1544
FauI CCCGC 1 cut(s) 1211
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 1 cut(s) 1425
FokI GGATG 1 cut(s) 802
FriOI GRGCYC 2 cut(s) 648, 1251
Fsp4HI GCNGC 1 cut(s) 1425
FspBI CTAG 2 cut(s) 1118, 1175
GluI GCNGC 1 cut(s) 1425
GsuI CTGGAG 2 cut(s) 266, 337
HaeIII GGCC 1 cut(s) 358
HgaI GACGC 1 cut(s) 1052
HinfI GANTC 7 cut(s) 250, 336, 502, 1090, 1106, 1212, 1490
HphI GGTGA 6 cut(s) 25, 162, 311, 717, 1017, 1192
Hpy166II GTNNAC 3 cut(s) 16, 532, 725
Hpy188I TCNGA 3 cut(s) 335, 699, 1041
Hpy188III TCNNGA 9 cut(s) 491, 558, 758, 927, 996, 1118, 1411, 1439, 1545
Hpy8I GTNNAC 3 cut(s) 16, 532, 725
HpyAV CCTTC 2 cut(s) 256, 1390
HpyCH4III ACNGT 3 cut(s) 1156, 1264, 1418
HpyCH4IV ACGT 1 cut(s) 769
HpyF10VI GCNNNNNNNGC 1 cut(s) 1157
HpyF3I CTNAG 5 cut(s) 287, 512, 1208, 1409, 1520
HpySE526I ACGT 1 cut(s) 769
Kzo9I GATC 4 cut(s) 882, 1018, 1369, 1480
LmnI GCTCC 3 cut(s) 54, 844, 1246
Lsp1109I GCAGC 1 cut(s) 1436
LweI GCATC 3 cut(s) 568, 1270, 1309
MaeI CTAG 2 cut(s) 1118, 1175
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 3 cut(s) 777, 1514, 1542
MalI GATC 4 cut(s) 884, 1020, 1371, 1482
MboI GATC 4 cut(s) 882, 1018, 1369, 1480
MflI RGATCY 2 cut(s) 882, 1480
MhlI GDGCHC 2 cut(s) 648, 1251
MluCI AATT 9 cut(s) 317, 419, 535, 540, 613, 745, 875, 933, 1269
MlyI GAGTC 2 cut(s) 511, 1099
MmeI TCCRAC 1 cut(s) 1246
Mph1103I ATGCAT 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 1196
MseI TTAA 6 cut(s) 414, 594, 713, 938, 954, 1535
MslI CAYNNNNRTG 2 cut(s) 216, 1169
MspR9I CCNGG 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 1157
NcoI CCATGG 3 cut(s) 810, 1142, 1237
NdeII GATC 4 cut(s) 882, 1018, 1369, 1480
NheI GCTAGC 1 cut(s) 1174
NlaIV GGNNCC 3 cut(s) 621, 840, 1248
NmuCI GTSAC 1 cut(s) 1542
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 925
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 1 cut(s) 1196
PfeI GAWTC 5 cut(s) 250, 336, 1106, 1212, 1490
PflMI CCANNNNNTGG 2 cut(s) 1034, 1243
PkrI GCNGC 1 cut(s) 1426
PleI GAGTC 2 cut(s) 510, 1098
PmaCI CACGTG 1 cut(s) 770
PmlI CACGTG 1 cut(s) 770
PpsI GAGTC 2 cut(s) 510, 1098
Ppu21I YACGTR 1 cut(s) 770
PpuMI RGGWCCY 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 353
PspCI CACGTG 1 cut(s) 770
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 621, 840, 1248
PspPI GGNCC 3 cut(s) 306, 357, 460
PspPPI RGGWCCY 1 cut(s) 460
PstNI CAGNNNCTG 2 cut(s) 629, 1416
PsuI RGATCY 2 cut(s) 882, 1480
RseI CAYNNNNRTG 2 cut(s) 216, 1169
SaqAI TTAA 6 cut(s) 414, 594, 713, 938, 954, 1535
SatI GCNGC 1 cut(s) 1425
Sau3AI GATC 4 cut(s) 882, 1018, 1369, 1480
Sau96I GGNCC 3 cut(s) 306, 357, 460
SchI GAGTC 2 cut(s) 511, 1099
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 2 cut(s) 648, 1251
SfaNI GCATC 3 cut(s) 568, 1270, 1309
SfcI CTRYAG 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 925
SinI GGWCC 2 cut(s) 306, 460
SlaI CTCGAG 1 cut(s) 925
SmiMI CAYNNNNRTG 2 cut(s) 216, 1169
SmlI CTYRAG 1 cut(s) 925
SmoI CTYRAG 1 cut(s) 925
Sse9I AATT 9 cut(s) 317, 419, 535, 540, 613, 745, 875, 933, 1269
SsiI CCGC 4 cut(s) 96, 176, 409, 1204
SspMI CTAG 2 cut(s) 1118, 1175
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 4 cut(s) 360, 810, 1142, 1237
TaaI ACNGT 3 cut(s) 1156, 1264, 1418
TaiI ACGT 1 cut(s) 772
TaqI TCGA 4 cut(s) 557, 757, 926, 1093
TasI AATT 9 cut(s) 317, 419, 535, 540, 613, 745, 875, 933, 1269
TfiI GAWTC 5 cut(s) 250, 336, 1106, 1212, 1490
Tru1I TTAA 6 cut(s) 414, 594, 713, 938, 954, 1535
Tru9I TTAA 6 cut(s) 414, 594, 713, 938, 954, 1535
TscAI CASTG 5 cut(s) 483, 634, 1267, 1500, 1517
TseFI GTSAC 1 cut(s) 1542
TseI GCWGC 1 cut(s) 1424
Tsp45I GTSAC 1 cut(s) 1542
TspDTI ATGAA 8 cut(s) 234, 432, 512, 536, 617, 626, 1205, 1376
TspRI CASTG 5 cut(s) 483, 634, 1267, 1500, 1517
Van91I CCANNNNNTGG 2 cut(s) 1034, 1243
VpaK11BI GGWCC 2 cut(s) 306, 460
VspI ATTAAT 1 cut(s) 414
XapI RAATTY 4 cut(s) 419, 613, 875, 1269
XbaI TCTAGA 1 cut(s) 1117
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 1196
XspI CTAG 2 cut(s) 1118, 1175
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.