MD17G1186900.v1.1

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000325
Physical Location & Seq
Forward (+)
22340624 .. 22344843
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1186900.v1.1.491

Sequence Viewer

Length: 2397 bp
ATGACTGAAGATCTCAAACTTGGAATCGAGTTCAGTTCTCATGAGAGTGCATACGTAGCTTATGTCAAGTATGGTGCAAAACACGGTTTTGACGTGAGGAAGCATCATAGAAAGACAAATAAGATGGGTCTCGTATCAAGGGTAACTTATGTTTGTTCAAAACAAGGTCACCGGAGAAAGGATAATAAACGGTGTGAGAGTATAACTTATTCTTTACCAAGTTCAAGGGTTGGCTGTGAAGCGCATATGGTTTGCCTACTCATGAAAACTGGAAAGTTTAAGATTATATCGTTTAATGCAAGTCATAATCATGATTTGATTAAAGCACCCATGAAACACATGCTAAAGGTTAATCGTTGTTTATCTAGAGCTCAAAAGGGCCATGTTGCTGACGGGGAAAAGTCAAGGTCACCAATAAAAGAAACAGTGGAACTAGTGAGTAGAGAAGTTGAGGGGCAAGAGAATCTTGGTTCCGGGGATAACGATTATCAAAATTACCTACAGAGAAAGAGGAAGTTAAAAATGGAAAAGGGAGATGCTGGGGCCATATTGCAATACTTTCAAAATATGCGTGAAGATAACTCATCTTGTTTTTATTCAGTGCAGCTTGATGAGGATGATAGGATCACAAATATATTTTGGGCAGATGCACGTTCAGTTTGTGATTATAAAGCCTTCGGTGATGTTGTTTGTTTTGATACCACTTATCAGATAAACGAATATGGTAGGCCATTTGCACCATTGATTGGGGTTAATCACCATAAACAAATTGTAGTTTTTGGTGCAGCTTTACTATATGATGATAGCAGAGACTCTTTTAAGTGGCTGTTTGAGACTTTTCTTGCTGCAATGTCTGGGAAGCAACCGAAGACCATACTTACAGACCAATCTGCTGCAACGGCAGAGGCAATATTAGAAGTTTTTCCTGAAGCTAACCATCGGCTATGTGCTTGGCATATTTACCAGAAAGCTGCCAAGAATTTAAGCCATGTGTTTCATGCATCAAAGCAATTTGCTTGTGATCTTAGCAAGTGTGTATATGAATATGAGGATAAAGATGCATGGCTTTCTGCATGGAATGATATGCTTGAAAAGTATGATCTCAAGGAGGATAATTGGTTAAAAGAAATATATGAAGTGAGGGAAAAATGGGCATTGGTGTATGAACGACACACTTTCACTGCAGACTTGATGAGTATACAACACAAGGAAAGTATCACCAATGTGTTGAAAAAACACTTGATGCCAAGTCATGATCTTTTGTGCTTCTTTGAACATTTTGAGAATATTTTGGCAGATCGACGAGATAAGGAATTACAAGCTGATTTCAAGATGATGCAGACCGTACCTTCACCAGTGATTAATGTGGAGATGTTAGAGCATGCAGTGGAGGTTTATACCCCAGAAGTTTTTAGACTCTTTGAAAAAGAGTACATGGGAATTCTTAATTGCAGTGTCTATAAAATTGGTAAGTCTGGGACGACCAGTGAATACAAAGTATGTTATAGATCTCATGAGCATTTGGTTAAATATGATAGTTCAACACAAGCAGTTAATTGTAGTTGCATGAAGTTCACTTTTGTTGGGATTTTATGCTCTCATGCATTGAAAGTGCTAGATAAGAAGAATGTGAAAACAATCCCACCTGATTACATATTGAAGCGCTGGACAAGAGGTGCAAGGGATAGGAGCCTCACTAGTTACCATGGAATTGAAACTAGCGACAATCCAAAGGAGTCTCCTGGGAAGCGTTATAGCCATTTGTGCCATAATTTTCGTGAGATAGCATCCCTTGCAGCAGAAGATGAGAGGTTAACTGCATGTGCACACGATTGGTCAGTTCAGTTGCTTAAAAGTTTAGAGGAAATGAAGAAAAATCTTGGCCTAGATAATGTACCTGTTCATAAAAATTCAGAAGGCGAAGCTTTAGTTGGAGAAAGTGGTGGAACTAACCAGGTGAAAATGGTAAAGCACAATGATTGTGAAATAACAAAAGAACGTGGAGTTAAAAGAAAGTATACAGCGAGATGCCCATGTAGTAGACTGACTGAGCCAGCGGAAACTCAGAAGCATATACCACCAACCAAAGGCACACAATCTGCAGCAAGACATTGGCTGTTAGAGGGATCCTCTGTTGGAGCTAGAACCCAAGTATCATTACCAAGTCATAGATTTGATGCAAGTGCAACTGTCAATTTGCAGGGTACTCTGCCTTTCACTCAACGTCTTCAGGGGTCGTATGTTGATAATGGAAACCAAGCGTCATATTCAAGTAGAACTTTTGGCGCCACTCCAAGTTTCCTCAATAACATGCATTTCACTCAACTATGTCAGGAACCTTCGAGGCATCATAATCACTGTTCACCAAGTAAGGGAGGCAAAGAGTCAGGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

799

Amino Acids

91.07

Weight (kDa)

8.58

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 21 - 107 1.5e-15 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 190 - 312 6.8e-11 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 229 - 323 6.9e-28 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24391 FvH4_2g38071 FvH4_3g07131 FvH4_3g22481 FvH4_4g15809 FvH4_5g37230 FvH4_7g25201 FvH4_7g29092 FvH4_7g29093
malus_domestica MD01G1197600.v1.1 MD17G1186900.v1.1
prunus_persica Prupe.1G227400_v2.0.a1 Prupe.2G291000_v2.0.a1 Prupe.4G268600_v2.0.a1 Prupe.5G094100_v2.0.a1
pyrus_communis pycom01g20780 pycom17g19620
rosa_chinensis RchiOBHm_Chr1g0376821 RchiOBHm_Chr5g0061941 RchiOBHm_Chr5g0078991 RchiOBHm_Chr6g0276821 RchiOBHm_Chr7g0191321
rosa_laevigata RLG00000007883 RLG00000008804 RLG00000010632 RLG00000026567 RLG00000035244 RLG00000036018 RLG00000036909
rosa_multiflora Rmu_sc0001036.1_g000026 Rmu_sc0001556.1_g000021 Rmu_sc0001974.1_g000022 Rmu_sc0002113.1_g000006 Rmu_sc0002572.1_g000008 Rmu_sc0004808.1_g000011 Rmu_sc0005117.1_g000017 Rmu_sc0005319.1_g000016 Rmu_sc0008432.1_g000005 Rmu_sc0010475.1_g000001 Rmu_sc0015522.1_g000003 Rmu_ssc0000066.1_g000009 Rmu_ssc0000400.1_g000102 Rmu_ssc0000400.1_g000103
rosa_roxburghii Rroxscaffold_1G00071290 Rroxscaffold_2G00141280 Rroxscaffold_3G00233790 Rroxscaffold_4G00297530 Rroxscaffold_5G00368860 Rroxscaffold_5G00386220 Rroxscaffold_7G00159400
rosa_rugosa Rorug01G0146800.1 Rorug01G0162100.1 Rorug01G0162200.1 Rorug01G0398600 Rorug01G0398700 Rorug02G0344200 Rorug02G0344300 Rorug02G0344400 Rorug03G0046200 Rorug03G0153200 Rorug04G0182500 Rorug04G0182600 Rorug05G0257700 Rorug05G0386400 Rorug05G0386400 Rorug05G0386500 Rorug05G0460900.1 Rorug05G0467000 Rorug07G0110400
rosa_samantha Rh1AG183500 Rh1AG416000 Rh1BG023600 Rh1BG145400 Rh1BG145500 Rh1BG278800 Rh1BG375400 Rh1CG389200 Rh1DG166300 Rh1DG176900 Rh1DG309900 Rh1DG406000 Rh2BG439100 Rh2BG502200 Rh2CG314800 Rh2CG517400 Rh2CG532000 Rh2DG449100 Rh3BG109900 Rh3CG230400 Rh3CG246600 Rh3CG289400 Rh3CG289500 Rh3DG120600 Rh4AG144400 Rh4AG408200 Rh4BG419300 Rh4CG101900 Rh4CG151000 Rh4CG226900 Rh4DG235400 Rh5AG265900 Rh5AG375200 Rh5CG559600 Rh5DG218200 Rh6AG074500 Rh6BG291800 Rh6CG292800 Rh6DG284900
rosa_wichuraiana Rw0G013240 Rw1G036490 Rw5G024920 Rw5G030730 Rw5G033710 Rw6G033840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccB1I GGYRCC 1 cut(s) 2285
AccB7I CCANNNNNTGG 1 cut(s) 746
AccI GTMKAC 3 cut(s) 1198, 2018, 2041
AciI CCGC 1 cut(s) 2057
AclWI GGATC 3 cut(s) 632, 2121, 2134
AcsI RAATTY 3 cut(s) 979, 1440, 1909
AcuI CTGAAG 3 cut(s) 27, 948, 2213
AcyI GRCGYC 1 cut(s) 2286
AfaI GTAC 4 cut(s) 1347, 1433, 1896, 2206
AfeI AGCGCT 1 cut(s) 1664
AfiI CCNNNNNNNGG 4 cut(s) 178, 746, 2087, 2372
AhlI ACTAGT 2 cut(s) 433, 1697
AjiI CACGTC 1 cut(s) 94
AjnI CCWGG 2 cut(s) 1741, 1953
AjuI GAANNNNNNNTTGG 2 cut(s) 1914, 1946
AleI CACNNNNGTG 1 cut(s) 1223
AluBI AGCT 9 cut(s) 59, 371, 607, 788, 932, 971, 1322, 1925, 2141
AluI AGCT 9 cut(s) 59, 371, 607, 788, 932, 971, 1322, 1925, 2141
Alw21I GWGCWC 2 cut(s) 373, 1828
Alw26I GTCTC 4 cut(s) 134, 804, 827, 1743
Alw44I GTGCAC 1 cut(s) 1824
AlwI GGATC 3 cut(s) 632, 2121, 2134
Aor51HI AGCGCT 1 cut(s) 1664
AoxI GGCC 4 cut(s) 379, 543, 728, 1882
ApaLI GTGCAC 1 cut(s) 1824
ApeKI GCWGC 7 cut(s) 604, 785, 845, 893, 971, 1796, 2102
ApoI RAATTY 3 cut(s) 979, 1440, 1909
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 1 cut(s) 921
AspLEI GCGC 3 cut(s) 244, 1665, 2288
AspS9I GGNCC 2 cut(s) 379, 543
AsuC2I CCSGG 1 cut(s) 475
AsuHPI GGTGA 8 cut(s) 161, 402, 692, 749, 1210, 1344, 1969, 2355
BaeGI GKGCMC 1 cut(s) 1828
BaeI ACNNNNGTAYC 2 cut(s) 2136, 2169
BamHI GGATCC 1 cut(s) 2126
BanI GGYRCC 1 cut(s) 2285
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 2 cut(s) 875, 2219
Bbv12I GWGCWC 2 cut(s) 373, 1828
BbvI GCAGC 7 cut(s) 616, 797, 832, 880, 958, 1808, 2114
BccI CCATC 2 cut(s) 118, 945
BceAI ACGGC 1 cut(s) 915
BciT130I CCWGG 2 cut(s) 1743, 1955
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 4 cut(s) 134, 804, 827, 1743
BcuI ACTAGT 2 cut(s) 433, 1697
BfaI CTAG 7 cut(s) 366, 434, 1616, 1698, 1719, 1886, 2142
BfmI CTRYAG 3 cut(s) 500, 1182, 2100
BfoI RGCGCY 2 cut(s) 1666, 2289
BglII AGATCT 2 cut(s) 10, 1508
BisI GCNGC 7 cut(s) 605, 786, 846, 894, 972, 1797, 2103
BlsI GCNGC 7 cut(s) 606, 787, 847, 895, 973, 1798, 2104
Bme1390I CCNGG 3 cut(s) 475, 1743, 1955
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 379, 543
BmiI GGNNCC 6 cut(s) 472, 544, 1691, 2128, 2287, 2337
BmrFI CCNGG 3 cut(s) 475, 1743, 1955
BpiI GAAGAC 2 cut(s) 875, 2219
BplI GAGNNNNNCTC 2 cut(s) 2114, 2146
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 1 cut(s) 475
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 2286
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 474, 1705, 1742
BsaWI WCCGGW 1 cut(s) 171
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 746, 2087, 2372
Bse1I ACTGG 3 cut(s) 274, 1355, 1485
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 2 cut(s) 1743, 1955
BseDI CCNNGG 3 cut(s) 474, 1705, 1742
BseGI GGATG 2 cut(s) 622, 1787
BseLI CCNNNNNNNGG 4 cut(s) 178, 746, 2087, 2372
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 2040, 2078
BseNI ACTGG 3 cut(s) 274, 1355, 1485
BseSI GKGCMC 1 cut(s) 1828
BseXI GCAGC 7 cut(s) 616, 797, 832, 880, 958, 1808, 2114
BseYI CCCAGC 1 cut(s) 539
BsgI GTGCAG 2 cut(s) 623, 804
BshFI GGCC 4 cut(s) 381, 545, 730, 1884
BshNI GGYRCC 1 cut(s) 2285
BsiHKAI GWGCWC 2 cut(s) 373, 1828
BsiSI CCGG 2 cut(s) 172, 474
BslFI GGGAC 1 cut(s) 1492
BslI CCNNNNNNNGG 4 cut(s) 178, 746, 2087, 2372
BsmAI GTCTC 4 cut(s) 134, 804, 827, 1743
BsmFI GGGAC 1 cut(s) 1492
BsnI GGCC 4 cut(s) 381, 545, 730, 1884
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 373, 1828
Bsp143I GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
Bsp19I CCATGG 1 cut(s) 1705
BspACI CCGC 1 cut(s) 2057
BspANI GGCC 4 cut(s) 381, 545, 730, 1884
BspCNI CTCAG 2 cut(s) 2041, 2077
BspHI TCATGA 5 cut(s) 40, 261, 310, 1252, 1513
BspLI GGNNCC 6 cut(s) 472, 544, 1691, 2128, 2287, 2337
BspMAI CTGCAG 2 cut(s) 1186, 2104
BspPI GGATC 3 cut(s) 632, 2121, 2134
BspT107I GGYRCC 1 cut(s) 2285
BspTNI GGTCTC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 3 cut(s) 274, 1355, 1485
BssECI CCNNGG 3 cut(s) 474, 1705, 1742
BssMI GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
BssNAI GTATAC 2 cut(s) 1199, 2019
BssNI GRCGYC 1 cut(s) 2286
BssT1I CCWWGG 1 cut(s) 1705
Bst1107I GTATAC 2 cut(s) 1199, 2019
Bst2UI CCWGG 2 cut(s) 1743, 1955
Bst4CI ACNGT 6 cut(s) 86, 192, 427, 1345, 2191, 2360
BstACI GRCGYC 1 cut(s) 2286
BstAPI GCANNNNNTGC 1 cut(s) 1793
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 1383, 2055
BstDEI CTNAG 3 cut(s) 1025, 2049, 2064
BstDSI CCRYGG 1 cut(s) 1705
BstEII GGTNACC 2 cut(s) 167, 408
BstF5I GGATG 2 cut(s) 622, 1787
BstH2I RGCGCY 2 cut(s) 1666, 2289
BstHHI GCGC 3 cut(s) 244, 1665, 2288
BstKTI GATC 8 cut(s) 13, 627, 1024, 1102, 1258, 1300, 1511, 2129
BstMAI GTCTC 4 cut(s) 134, 804, 827, 1743
BstMBI GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
BstMWI GCNNNNNNNGC 4 cut(s) 56, 899, 1764, 1793
BstNI CCWGG 2 cut(s) 1743, 1955
BstNSI RCATGY 4 cut(s) 343, 1385, 1824, 2314
BstPI GGTNACC 2 cut(s) 167, 408
BstSCI CCNGG 3 cut(s) 473, 1741, 1953
BstSFI CTRYAG 3 cut(s) 500, 1182, 2100
BstSLI GKGCMC 1 cut(s) 1828
BstSNI TACGTA 1 cut(s) 55
BstV1I GCAGC 7 cut(s) 616, 797, 832, 880, 958, 1808, 2114
BstV2I GAAGAC 2 cut(s) 875, 2219
BstX2I RGATCY 3 cut(s) 10, 1508, 2126
BstYI RGATCY 3 cut(s) 10, 1508, 2126
BstZ17I GTATAC 2 cut(s) 1199, 2019
BsuRI GGCC 4 cut(s) 381, 545, 730, 1884
BtgI CCRYGG 1 cut(s) 1705
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 2 cut(s) 622, 1787
BtsI GCAGTG 3 cut(s) 1179, 1392, 1459
BtsIMutI CAGTG 8 cut(s) 432, 606, 1179, 1362, 1392, 1459, 1492, 2356
Cac8I GCNNGC 2 cut(s) 1383, 2055
CciI TCATGA 5 cut(s) 40, 261, 310, 1252, 1513
CfoI GCGC 3 cut(s) 244, 1665, 2288
Cfr13I GGNCC 2 cut(s) 379, 543
CseI GACGC 1 cut(s) 2250
CsiI ACCWGGT 1 cut(s) 1953
Csp6I GTAC 4 cut(s) 1346, 1432, 1895, 2205
CviQI GTAC 4 cut(s) 1346, 1432, 1895, 2205
DdeI CTNAG 3 cut(s) 1025, 2049, 2064
DinI GGCGCC 1 cut(s) 2287
DpnI GATC 8 cut(s) 12, 626, 1023, 1101, 1257, 1299, 1510, 2128
DpnII GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
Ecl136II GAGCTC 1 cut(s) 371
Eco105I TACGTA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 1705
Eco24I GRGCYC 1 cut(s) 373
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 1664
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 3 cut(s) 27, 948, 2213
Eco91I GGTNACC 2 cut(s) 167, 408
EcoICRI GAGCTC 1 cut(s) 371
EcoO65I GGTNACC 2 cut(s) 167, 408
EcoRI GAATTC 1 cut(s) 1440
EcoRII CCWGG 2 cut(s) 1741, 1953
EcoT14I CCWWGG 1 cut(s) 1705
EcoT22I ATGCAT 4 cut(s) 1003, 1063, 1606, 2316
EcoT38I GRGCYC 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 2287
EheI GGCGCC 1 cut(s) 2287
ErhI CCWWGG 1 cut(s) 1705
FalI AAGNNNNNCTT 6 cut(s) 130, 162, 450, 482, 2263, 2295
FaqI GGGAC 1 cut(s) 1492
FauNDI CATATG 1 cut(s) 246
FblI GTMKAC 3 cut(s) 1198, 2018, 2041
Fnu4HI GCNGC 7 cut(s) 605, 786, 846, 894, 972, 1797, 2103
FokI GGATG 2 cut(s) 629, 1774
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 7 cut(s) 605, 786, 846, 894, 972, 1797, 2103
FspBI CTAG 7 cut(s) 366, 434, 1616, 1698, 1719, 1886, 2142
GlaI GCGC 3 cut(s) 243, 1664, 2287
GluI GCNGC 7 cut(s) 605, 786, 846, 894, 972, 1797, 2103
GsaI CCCAGC 1 cut(s) 543
HaeII RGCGCY 2 cut(s) 1666, 2289
HaeIII GGCC 4 cut(s) 381, 545, 730, 1884
HapII CCGG 2 cut(s) 172, 474
HgaI GACGC 1 cut(s) 2250
HhaI GCGC 3 cut(s) 244, 1665, 2288
Hin1I GRCGYC 1 cut(s) 2286
Hin6I GCGC 3 cut(s) 242, 1663, 2286
HinP1I GCGC 3 cut(s) 242, 1663, 2286
HincII GTYRAC 1 cut(s) 1815
HindII GTYRAC 1 cut(s) 1815
HindIII AAGCTT 1 cut(s) 1923
HinfI GANTC 6 cut(s) 24, 463, 812, 1416, 1736, 2384
HpaI GTTAAC 1 cut(s) 1815
HpaII CCGG 2 cut(s) 172, 474
HphI GGTGA 8 cut(s) 161, 402, 692, 749, 1210, 1344, 1969, 2355
Hpy166II GTNNAC 7 cut(s) 1199, 1575, 1815, 1826, 2019, 2042, 2363
Hpy188I TCNGA 3 cut(s) 711, 1915, 2067
Hpy8I GTNNAC 7 cut(s) 1199, 1575, 1815, 1826, 2019, 2042, 2363
Hpy99I CGWCG 1 cut(s) 1305
HpyAV CCTTC 4 cut(s) 685, 1359, 1910, 2349
HpyCH4III ACNGT 6 cut(s) 86, 192, 427, 1345, 2191, 2360
HpyCH4IV ACGT 5 cut(s) 54, 93, 652, 1999, 2224
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 899, 1764, 1793
HpyF3I CTNAG 3 cut(s) 1025, 2049, 2064
HpySE526I ACGT 5 cut(s) 54, 93, 652, 1999, 2224
Hsp92I GRCGYC 1 cut(s) 2286
HspAI GCGC 3 cut(s) 242, 1663, 2286
KasI GGCGCC 1 cut(s) 2285
KspAI GTTAAC 1 cut(s) 1815
Kzo9I GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
LmnI GCTCC 2 cut(s) 1689, 2138
Lsp1109I GCAGC 7 cut(s) 616, 797, 832, 880, 958, 1808, 2114
MabI ACCWGGT 1 cut(s) 1953
MaeI CTAG 7 cut(s) 366, 434, 1616, 1698, 1719, 1886, 2142
MaeII ACGT 5 cut(s) 54, 93, 652, 1999, 2224
MaeIII GTNAC 4 cut(s) 142, 167, 408, 1700
MalI GATC 8 cut(s) 12, 626, 1023, 1101, 1257, 1299, 1510, 2128
MboI GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
MboII GAAGA 7 cut(s) 20, 587, 880, 1636, 1814, 1882, 2219
MflI RGATCY 3 cut(s) 10, 1508, 2126
MhlI GDGCHC 2 cut(s) 373, 1828
Mly113I GGCGCC 1 cut(s) 2286
MlyI GAGTC 4 cut(s) 806, 1410, 1745, 2393
MmeI TCCRAC 2 cut(s) 1912, 2116
Mph1103I ATGCAT 4 cut(s) 1003, 1063, 1606, 2316
MroXI GAANNNNTTC 1 cut(s) 921
MslI CAYNNNNRTG 3 cut(s) 45, 309, 1223
MspA1I CMGCKG 1 cut(s) 2057
MspI CCGG 2 cut(s) 172, 474
MspR9I CCNGG 3 cut(s) 475, 1743, 1955
MvaI CCWGG 2 cut(s) 1743, 1955
MwoI GCNNNNNNNGC 4 cut(s) 56, 899, 1764, 1793
NarI GGCGCC 1 cut(s) 2286
NciI CCSGG 1 cut(s) 475
NcoI CCATGG 1 cut(s) 1705
NdeI CATATG 1 cut(s) 246
NdeII GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
NlaIV GGNNCC 6 cut(s) 472, 544, 1691, 2128, 2287, 2337
NmuCI GTSAC 2 cut(s) 167, 408
NsiI ATGCAT 4 cut(s) 1003, 1063, 1606, 2316
NspI RCATGY 4 cut(s) 343, 1385, 1824, 2314
OliI CACNNNNGTG 1 cut(s) 1223
PaeI GCATGC 1 cut(s) 1385
PagI TCATGA 5 cut(s) 40, 261, 310, 1252, 1513
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 921
PfeI GAWTC 2 cut(s) 24, 463
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 7 cut(s) 606, 787, 847, 895, 973, 1798, 2104
PleI GAGTC 4 cut(s) 806, 1410, 1744, 2392
PluTI GGCGCC 1 cut(s) 2289
PpsI GAGTC 4 cut(s) 806, 1410, 1744, 2392
Ppu21I YACGTR 1 cut(s) 55
PshBI ATTAAT 1 cut(s) 1362
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 373
Psp6I CCWGG 2 cut(s) 1741, 1953
PspEI GGTNACC 2 cut(s) 167, 408
PspFI CCCAGC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 1741, 1953
PspN4I GGNNCC 6 cut(s) 472, 544, 1691, 2128, 2287, 2337
PspPI GGNCC 2 cut(s) 379, 543
PstI CTGCAG 2 cut(s) 1186, 2104
PsuI RGATCY 3 cut(s) 10, 1508, 2126
RsaI GTAC 4 cut(s) 1347, 1433, 1896, 2206
RsaNI GTAC 4 cut(s) 1346, 1432, 1895, 2205
RseI CAYNNNNRTG 3 cut(s) 45, 309, 1223
SacI GAGCTC 1 cut(s) 373
SatI GCNGC 7 cut(s) 605, 786, 846, 894, 972, 1797, 2103
Sau3AI GATC 8 cut(s) 10, 624, 1021, 1099, 1255, 1297, 1508, 2126
Sau96I GGNCC 2 cut(s) 379, 543
SchI GAGTC 4 cut(s) 806, 1410, 1745, 2393
ScrFI CCNGG 3 cut(s) 475, 1743, 1955
SduI GDGCHC 2 cut(s) 373, 1828
SexAI ACCWGGT 1 cut(s) 1953
SfcI CTRYAG 3 cut(s) 500, 1182, 2100
SfoI GGCGCC 1 cut(s) 2287
SmiMI CAYNNNNRTG 3 cut(s) 45, 309, 1223
SmlI CTYRAG 1 cut(s) 1103
SmoI CTYRAG 1 cut(s) 1103
SnaBI TACGTA 1 cut(s) 55
SpeI ACTAGT 2 cut(s) 433, 1697
SphI GCATGC 1 cut(s) 1385
SsiI CCGC 1 cut(s) 2057
SspDI GGCGCC 1 cut(s) 2285
SspI AATATT 2 cut(s) 912, 1288
SspMI CTAG 7 cut(s) 366, 434, 1616, 1698, 1719, 1886, 2142
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 3 cut(s) 473, 1741, 1953
StyI CCWWGG 1 cut(s) 1705
TaaI ACNGT 6 cut(s) 86, 192, 427, 1345, 2191, 2360
TaiI ACGT 5 cut(s) 57, 96, 655, 2002, 2227
TaqI TCGA 3 cut(s) 27, 1300, 2342
TatI WGTACW 1 cut(s) 1431
TfiI GAWTC 2 cut(s) 24, 463
TscAI CASTG 8 cut(s) 432, 606, 1186, 1362, 1392, 1459, 1492, 2363
TseFI GTSAC 2 cut(s) 167, 408
TseI GCWGC 7 cut(s) 604, 785, 845, 893, 971, 1796, 2102
Tsp45I GTSAC 2 cut(s) 167, 408
TspDTI ATGAA 9 cut(s) 278, 347, 986, 1056, 1149, 1179, 1583, 1883, 1892
TspRI CASTG 8 cut(s) 432, 606, 1186, 1362, 1392, 1459, 1492, 2363
Van91I CCANNNNNTGG 1 cut(s) 746
VneI GTGCAC 1 cut(s) 1824
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 3 cut(s) 979, 1440, 1909
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 4 cut(s) 343, 1385, 1824, 2314
XmiI GTMKAC 3 cut(s) 1198, 2018, 2041
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 7 cut(s) 366, 434, 1616, 1698, 1719, 1886, 2142
Zsp2I ATGCAT 4 cut(s) 1003, 1063, 1606, 2316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.