RchiOBHm_Chr6g0276821

Protein FAR1-RELATED SEQUENCE 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000325
Physical Location & Seq
Forward (+)
39144805 .. 39147035
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24833

Sequence Viewer

Length: 1824 bp
ATGGACCACAAATCTCCTGTGCCATCAACACCGGATGTGGAAGCTACGGATAGTTGTGATTGTCAAATATCTAGCAAATTGGAATTTGGGAGTGGTATAGAGTTGAATTGTTCTGTAGAAGTTGAAAATAATGAAGTTGGAGGTAGTGAAGAAAAGTCAGAAGAAGCCACAAAACCTATTGAAACGACTGATTCAATTCATTCTAGAATGCGTCAAGAATTGATTCCTGCAGTTGGTATGGAGTTTGAAACAGAACATGATGCAGATGCTTTTTACAATCAATATGCATATAGATTTGGTTTCAGTACCAGATTAAGTAAGGCACATAAATCCTCCAGTGGCCTATTGAGGGATAGGCTCTTTGTTTGCTCAGCCGAAGGTAAACGTGGAAGAGACAAGCGAAATCCCTATGTCAAATCTCATCGTGCTGAGACAAGATTTGGGTGTAGAGCTAAGATGAAAATTAGGTATGATCTGTTATCCAGAAAGTATAGTGTTGTGGAATTTGTTGCTGATCATACCCATGTGACTACAACTCCTAGTAAGACCCATCTTTTTAGGTCTCATAGGAAAATCTCTCTTGCACAAATTGTTCAAGCTGATATGGCTTACGATTCAGGAATCAATCCAAAAGAAACTCTTGAACTATTAAGTAGACAAGCTGGTGGACGTGAGCATTTGGGATTTATTCCAGAAGATTATAAGAATTATTTACGTTCAAAGCGAACAAGAGAAATGAAGTCAGGGGATACCGGTGGTGTTTTGGAATATCTACAAAGAATGCAGTTGAATGATCCTAATTTTGTTTATGCAATACAAGTGGATGAGGATGATTTGATAACCAATATTTTCTGGGCTGATGCAAAGATGATGGTAGACTATGATTACTTTGGTGATGTGGTAAGGTTTGATACCACTTACAGGAAAAATAAAGAAGGCAGACCTTTTGCGATGTTTGTTGGTGTCAATAATCATAAGCAAACACTTATATTTGGTGCTGCATTGTTATATGATGAGACTGCTGAAACTTTCATGTGGTTATTTGATACCTTTGCAAATACAATGTCTGGAAAGAAACCAAAGAGCATTTTGACTGACCAAGATCTTGCAATGGCTAAAGCATTAGCTTCACAATGGCCAGAAACACATCATCGACTATGCATTTGGCACATATATCAAAATGCAGCTAAGCATCTTAGTAGTGTGTTTGAGCAGTTTAAGAATTTTGCTGAAGACTTTAGCAGTATCATATATGATTATGAAGATGTTGAAGACTTTTTAATTGCTTGGAAGAAGATGCTTGAAAAATACAATCTCCAAGAAAATAAATGGTTAGGCCGGTTATTTGATTTGAAGGAGAAATGGGCCTTAGTTTATGGGCGAGAAACATTCTGCGCAGATATTACCACCACCCAACGCAGTGAAAGCATGAATAATTCTATCAAGAAGTATGTGAGTTACAAGTATGATTTGCTGCGATTTTTTCGTCATTTTCAGAGATTACTTGATGACCGTCGTTATAATGAGTCAGTAGCAAATTTTAAATCAAGTCAGACCACTCCTGCATTGTCATTCCCAGTGAAAATATTAAGGAATGCAGCAAAAGTGTACACACCAGCAGTGTTTAAATGGTTCCAAGTTGAGTTATGTAAAGCTCATGATTGTACCTTGAAGTTATTTGGTGAGATTGGAACGATGCGCATATATGAAGTTACTGCTCATGGAAAGCGTTTCCACCATACAGTAACATTTGACTCGGTTGATAATAATATCTCATGCACTTGTAAGAAGTTTGAGTTTGCTGGAATTTTGTACCATTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

607

Amino Acids

70.34

Weight (kDa)

7.3

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 91 - 176 1.8e-18 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 265 - 382 6.7e-13 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 300 - 394 2.2e-30 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24391 FvH4_2g38071 FvH4_3g07131 FvH4_3g22481 FvH4_4g15809 FvH4_5g37230 FvH4_7g25201 FvH4_7g29092 FvH4_7g29093
malus_domestica MD01G1197600.v1.1 MD17G1186900.v1.1
prunus_persica Prupe.1G227400_v2.0.a1 Prupe.2G291000_v2.0.a1 Prupe.4G268600_v2.0.a1 Prupe.5G094100_v2.0.a1
pyrus_communis pycom01g20780 pycom17g19620
rosa_chinensis RchiOBHm_Chr1g0376821 RchiOBHm_Chr5g0061941 RchiOBHm_Chr5g0078991 RchiOBHm_Chr6g0276821 RchiOBHm_Chr7g0191321
rosa_laevigata RLG00000007883 RLG00000008804 RLG00000010632 RLG00000026567 RLG00000035244 RLG00000036018 RLG00000036909
rosa_multiflora Rmu_sc0001036.1_g000026 Rmu_sc0001556.1_g000021 Rmu_sc0001974.1_g000022 Rmu_sc0002113.1_g000006 Rmu_sc0002572.1_g000008 Rmu_sc0004808.1_g000011 Rmu_sc0005117.1_g000017 Rmu_sc0005319.1_g000016 Rmu_sc0008432.1_g000005 Rmu_sc0010475.1_g000001 Rmu_sc0015522.1_g000003 Rmu_ssc0000066.1_g000009 Rmu_ssc0000400.1_g000102 Rmu_ssc0000400.1_g000103
rosa_roxburghii Rroxscaffold_1G00071290 Rroxscaffold_2G00141280 Rroxscaffold_3G00233790 Rroxscaffold_4G00297530 Rroxscaffold_5G00368860 Rroxscaffold_5G00386220 Rroxscaffold_7G00159400
rosa_rugosa Rorug01G0146800.1 Rorug01G0162100.1 Rorug01G0162200.1 Rorug01G0398600 Rorug01G0398700 Rorug02G0344200 Rorug02G0344300 Rorug02G0344400 Rorug03G0046200 Rorug03G0153200 Rorug04G0182500 Rorug04G0182600 Rorug05G0257700 Rorug05G0386400 Rorug05G0386400 Rorug05G0386500 Rorug05G0460900.1 Rorug05G0467000 Rorug07G0110400
rosa_samantha Rh1AG183500 Rh1AG416000 Rh1BG023600 Rh1BG145400 Rh1BG145500 Rh1BG278800 Rh1BG375400 Rh1CG389200 Rh1DG166300 Rh1DG176900 Rh1DG309900 Rh1DG406000 Rh2BG439100 Rh2BG502200 Rh2CG314800 Rh2CG517400 Rh2CG532000 Rh2DG449100 Rh3BG109900 Rh3CG230400 Rh3CG246600 Rh3CG289400 Rh3CG289500 Rh3DG120600 Rh4AG144400 Rh4AG408200 Rh4BG419300 Rh4CG101900 Rh4CG151000 Rh4CG226900 Rh4DG235400 Rh5AG265900 Rh5AG375200 Rh5CG559600 Rh5DG218200 Rh6AG074500 Rh6BG291800 Rh6CG292800 Rh6DG284900
rosa_wichuraiana Rw0G013240 Rw1G036490 Rw5G024920 Rw5G030730 Rw5G033710 Rw6G033840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 702, 1521
Acc16I TGCGCA 2 cut(s) 1396, 1700
AccI GTMKAC 2 cut(s) 655, 876
AclWI GGATC 1 cut(s) 788
AcoI YGGCCR 1 cut(s) 1136
AcsI RAATTY 5 cut(s) 83, 503, 1222, 1537, 1806
AcuI CTGAAG 1 cut(s) 1251
AfaI GTAC 4 cut(s) 307, 1610, 1666, 1814
AfiI CCNNNNNNNGG 3 cut(s) 233, 349, 921
AgeI ACCGGT 1 cut(s) 752
AjiI CACGTC 1 cut(s) 671
AluBI AGCT 7 cut(s) 44, 452, 599, 662, 1127, 1187, 1655
AluI AGCT 7 cut(s) 44, 452, 599, 662, 1127, 1187, 1655
Alw26I GTCTC 4 cut(s) 387, 425, 567, 1010
AlwI GGATC 1 cut(s) 788
AoxI GGCC 4 cut(s) 340, 1136, 1336, 1365
ApeKI GCWGC 4 cut(s) 998, 1184, 1474, 1598
ApoI RAATTY 5 cut(s) 83, 503, 1222, 1537, 1806
ArsI GACNNNNNNTTYG 4 cut(s) 1049, 1081, 1146, 1178
AsiGI ACCGGT 1 cut(s) 752
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 2 cut(s) 1397, 1701
AspS9I GGNCC 2 cut(s) 4, 1365
AsuHPI GGTGA 2 cut(s) 905, 1694
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 1138
BbsI GAAGAC 2 cut(s) 1239, 1278
BbvI GCAGC 4 cut(s) 985, 1196, 1461, 1610
BccI CCATC 3 cut(s) 31, 558, 865
BciVI GTATCC 1 cut(s) 742
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 4 cut(s) 387, 425, 567, 1010
BfaI CTAG 4 cut(s) 72, 204, 540, 1822
BfmI CTRYAG 2 cut(s) 114, 228
BfuI GTATCC 1 cut(s) 742
BglII AGATCT 1 cut(s) 1102
BisI GCNGC 4 cut(s) 999, 1185, 1475, 1599
BlpI GCTNAGC 2 cut(s) 370, 1188
BlsI GCNGC 4 cut(s) 1000, 1186, 1476, 1600
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 671
BmgT120I GGNCC 2 cut(s) 4, 1365
BmiI GGNNCC 1 cut(s) 1634
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 6 cut(s) 250, 256, 850, 1201, 1287, 1686
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 2 cut(s) 1239, 1278
BpmI CTGGAG 1 cut(s) 319
Bpu1102I GCTNAGC 2 cut(s) 370, 1188
BsaI GGTCTC 1 cut(s) 567
BsaWI WCCGGW 2 cut(s) 31, 752
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 349, 921
Bse118I RCCGGY 2 cut(s) 752, 1338
Bse1I ACTGG 2 cut(s) 336, 1577
Bse3DI GCAATG 1 cut(s) 1116
BseGI GGATG 3 cut(s) 40, 829, 835
BseLI CCNNNNNNNGG 3 cut(s) 233, 349, 921
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 2 cut(s) 384, 420
BseNI ACTGG 2 cut(s) 336, 1577
BseXI GCAGC 4 cut(s) 985, 1196, 1461, 1610
BshFI GGCC 4 cut(s) 342, 1138, 1338, 1367
BshTI ACCGGT 1 cut(s) 752
BsiSI CCGG 3 cut(s) 32, 753, 1339
BslI CCNNNNNNNGG 3 cut(s) 233, 349, 921
BsmAI GTCTC 4 cut(s) 387, 425, 567, 1010
BsmI GAATGC 3 cut(s) 213, 786, 1600
BsnI GGCC 4 cut(s) 342, 1138, 1338, 1367
Bso31I GGTCTC 1 cut(s) 567
Bsp1407I TGTACA 1 cut(s) 1608
Bsp143I GATC 4 cut(s) 472, 514, 793, 1102
Bsp1720I GCTNAGC 2 cut(s) 370, 1188
BspANI GGCC 4 cut(s) 342, 1138, 1338, 1367
BspCNI CTCAG 2 cut(s) 383, 421
BspHI TCATGA 1 cut(s) 1657
BspLI GGNNCC 1 cut(s) 1634
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 1116
BsrFI RCCGGY 2 cut(s) 752, 1338
BsrGI TGTACA 1 cut(s) 1608
BsrI ACTGG 2 cut(s) 336, 1577
BssAI RCCGGY 2 cut(s) 752, 1338
BssMI GATC 4 cut(s) 472, 514, 793, 1102
Bst4CI ACNGT 2 cut(s) 1514, 1744
Bst6I CTCTTC 1 cut(s) 385
BstAUI TGTACA 1 cut(s) 1608
BstDEI CTNAG 6 cut(s) 370, 429, 453, 1188, 1196, 1369
BstF5I GGATG 3 cut(s) 40, 829, 835
BstHHI GCGC 2 cut(s) 1397, 1701
BstKTI GATC 4 cut(s) 475, 517, 796, 1105
BstMAI GTCTC 4 cut(s) 387, 425, 567, 1010
BstMBI GATC 4 cut(s) 472, 514, 793, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 605, 1425
BstSFI CTRYAG 2 cut(s) 114, 228
BstV1I GCAGC 4 cut(s) 985, 1196, 1461, 1610
BstV2I GAAGAC 2 cut(s) 1239, 1278
BstX2I RGATCY 1 cut(s) 1102
BstYI RGATCY 1 cut(s) 1102
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 4 cut(s) 342, 1138, 1338, 1367
BtgZI GCGATG 1 cut(s) 965
BtrI CACGTC 1 cut(s) 671
BtsCI GGATG 3 cut(s) 40, 829, 835
BtsI GCAGTG 2 cut(s) 1426, 1626
BtsIMutI CAGTG 4 cut(s) 343, 1426, 1584, 1626
CciI TCATGA 1 cut(s) 1657
CfoI GCGC 2 cut(s) 1397, 1701
Cfr10I RCCGGY 2 cut(s) 752, 1338
Cfr13I GGNCC 2 cut(s) 4, 1365
CseI GACGC 1 cut(s) 200
Csp6I GTAC 4 cut(s) 306, 1609, 1665, 1813
CspAI ACCGGT 1 cut(s) 752
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 7 cut(s) 257, 524, 1033, 1429, 1658, 1721, 1776
CviQI GTAC 4 cut(s) 306, 1609, 1665, 1813
DdeI CTNAG 6 cut(s) 370, 429, 453, 1188, 1196, 1369
DpnI GATC 4 cut(s) 474, 516, 795, 1104
DpnII GATC 4 cut(s) 472, 514, 793, 1102
DraI TTTAAA 2 cut(s) 1543, 1627
EaeI YGGCCR 1 cut(s) 1136
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 1251
EcoT22I ATGCAT 2 cut(s) 289, 1163
FaeI CATG 7 cut(s) 260, 527, 1036, 1432, 1661, 1724, 1779
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FatI CATG 7 cut(s) 256, 523, 1032, 1428, 1657, 1720, 1775
FbaI TGATCA 1 cut(s) 514
FblI GTMKAC 2 cut(s) 655, 876
Fnu4HI GCNGC 4 cut(s) 999, 1185, 1475, 1599
FokI GGATG 3 cut(s) 47, 836, 842
Fsp4HI GCNGC 4 cut(s) 999, 1185, 1475, 1599
FspAI RTGCGCAY 1 cut(s) 1700
FspBI CTAG 4 cut(s) 72, 204, 540, 1822
FspI TGCGCA 2 cut(s) 1396, 1700
GlaI GCGC 2 cut(s) 1396, 1700
GluI GCNGC 4 cut(s) 999, 1185, 1475, 1599
GsuI CTGGAG 1 cut(s) 319
HaeIII GGCC 4 cut(s) 342, 1138, 1338, 1367
HapII CCGG 3 cut(s) 32, 753, 1339
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 1397, 1701
Hin1II CATG 7 cut(s) 260, 527, 1036, 1432, 1661, 1724, 1779
Hin6I GCGC 2 cut(s) 1395, 1699
HinP1I GCGC 2 cut(s) 1395, 1699
HinfI GANTC 6 cut(s) 191, 223, 614, 621, 1526, 1754
HpaII CCGG 3 cut(s) 32, 753, 1339
HphI GGTGA 2 cut(s) 905, 1694
Hpy166II GTNNAC 6 cut(s) 383, 656, 668, 877, 1609, 1611
Hpy188I TCNGA 3 cut(s) 160, 1497, 1554
Hpy188III TCNNGA 9 cut(s) 204, 215, 483, 618, 641, 692, 1068, 1444, 1658
Hpy8I GTNNAC 6 cut(s) 383, 656, 668, 877, 1609, 1611
Hpy99I CGWCG 1 cut(s) 1518
HpyAV CCTTC 3 cut(s) 371, 929, 1348
HpyCH4III ACNGT 2 cut(s) 1514, 1744
HpyCH4IV ACGT 3 cut(s) 385, 670, 715
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 1425
HpyF3I CTNAG 6 cut(s) 370, 429, 453, 1188, 1196, 1369
HpySE526I ACGT 3 cut(s) 385, 670, 715
Hsp92II CATG 7 cut(s) 260, 527, 1036, 1432, 1661, 1724, 1779
HspAI GCGC 2 cut(s) 1395, 1699
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 4 cut(s) 472, 514, 793, 1102
Lsp1109I GCAGC 4 cut(s) 985, 1196, 1461, 1610
LweI GCATC 6 cut(s) 250, 256, 850, 1201, 1287, 1686
MaeI CTAG 4 cut(s) 72, 204, 540, 1822
MaeII ACGT 3 cut(s) 385, 670, 715
MaeIII GTNAC 4 cut(s) 526, 1457, 1711, 1744
MalI GATC 4 cut(s) 474, 516, 795, 1104
MboI GATC 4 cut(s) 472, 514, 793, 1102
MboII GAAGA 9 cut(s) 161, 173, 402, 707, 1244, 1274, 1283, 1303, 1306
MflI RGATCY 1 cut(s) 1102
MlsI TGGCCA 1 cut(s) 1138
MluNI TGGCCA 1 cut(s) 1138
MlyI GAGTC 2 cut(s) 1535, 1748
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 4 cut(s) 134, 342, 343, 820
Mox20I TGGCCA 1 cut(s) 1138
Mph1103I ATGCAT 2 cut(s) 289, 1163
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 1138
MseI TTAA 7 cut(s) 314, 650, 1218, 1280, 1542, 1589, 1626
MslI CAYNNNNRTG 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 1138
MspI CCGG 3 cut(s) 32, 753, 1339
Mva1269I GAATGC 3 cut(s) 213, 786, 1600
MwoI GCNNNNNNNGC 2 cut(s) 605, 1425
NdeII GATC 4 cut(s) 472, 514, 793, 1102
NlaIII CATG 7 cut(s) 260, 527, 1036, 1432, 1661, 1724, 1779
NlaIV GGNNCC 1 cut(s) 1634
NmuCI GTSAC 1 cut(s) 526
NsbI TGCGCA 2 cut(s) 1396, 1700
NsiI ATGCAT 2 cut(s) 289, 1163
PagI TCATGA 1 cut(s) 1657
PcsI WCGNNNNNNNCGW 1 cut(s) 721
PctI GAATGC 3 cut(s) 213, 786, 1600
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 4 cut(s) 191, 223, 614, 621
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 4 cut(s) 1000, 1186, 1476, 1600
PleI GAGTC 2 cut(s) 1534, 1748
PpsI GAGTC 2 cut(s) 1534, 1748
PsiI TTATAA 2 cut(s) 702, 1521
PspN4I GGNNCC 1 cut(s) 1634
PspPI GGNCC 2 cut(s) 4, 1365
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 1102
RsaI GTAC 4 cut(s) 307, 1610, 1666, 1814
RsaNI GTAC 4 cut(s) 306, 1609, 1665, 1813
RseI CAYNNNNRTG 1 cut(s) 522
SaqAI TTAA 7 cut(s) 314, 650, 1218, 1280, 1542, 1589, 1626
SatI GCNGC 4 cut(s) 999, 1185, 1475, 1599
Sau3AI GATC 4 cut(s) 472, 514, 793, 1102
Sau96I GGNCC 2 cut(s) 4, 1365
SchI GAGTC 2 cut(s) 1535, 1748
SfaNI GCATC 6 cut(s) 250, 256, 850, 1201, 1287, 1686
SfcI CTRYAG 2 cut(s) 114, 228
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 522
SspI AATATT 2 cut(s) 847, 1587
SspMI CTAG 4 cut(s) 72, 204, 540, 1822
TaaI ACNGT 2 cut(s) 1514, 1744
TaiI ACGT 3 cut(s) 388, 673, 718
TaqI TCGA 1 cut(s) 1153
TatI WGTACW 1 cut(s) 1608
TfiI GAWTC 4 cut(s) 191, 223, 614, 621
Tru1I TTAA 7 cut(s) 314, 650, 1218, 1280, 1542, 1589, 1626
Tru9I TTAA 7 cut(s) 314, 650, 1218, 1280, 1542, 1589, 1626
TscAI CASTG 4 cut(s) 343, 1426, 1584, 1626
TseFI GTSAC 1 cut(s) 526
TseI GCWGC 4 cut(s) 998, 1184, 1474, 1598
Tsp45I GTSAC 1 cut(s) 526
TspDTI ATGAA 8 cut(s) 147, 188, 473, 752, 1021, 1275, 1445, 1722
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 4 cut(s) 343, 1426, 1584, 1626
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 5 cut(s) 83, 503, 1222, 1537, 1806
XbaI TCTAGA 1 cut(s) 203
XmiI GTMKAC 2 cut(s) 655, 876
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 4 cut(s) 72, 204, 540, 1822
Zsp2I ATGCAT 2 cut(s) 289, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.