pycom01g20780

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000325
Physical Location & Seq
Forward (+)
19122871 .. 19125763
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20780.3

Sequence Viewer

Length: 2529 bp
ATGGATGTCGGACTTCAAACGGCAAATAGGTTGGACTTGAATGTAGAGCAAGTTTTTTGCAGCCCAAAATTTGTATCTATCAATGCTACTCAGCCTAATATTTCTTCCATAGGTGCACACAGTATAGATTCAGTTTTAAAACTTGGTTCAGAATTCGAATCTGATGAACATGCTTACAAATCTTACAAGAACTATGCCAGGTTGGTGGGTTTTAATGTTCGAAAAGACTGGGTCAATAGGAGTAAGGTACATGGCCAGGTGGTATCGAGGAAGTTCACTTGTTCTAGAGAAGGTTATCGACAGAATGACAAAAGAGATGCTAATGTAAAGAAACGTCGCAAGGAAACAAGAACTGGTTGCTTGGCGCATGTTATTATTACTCGTCAACCTGATAGTAAATACCGGGTTACGCAATTTGAAGAACAACACAATCATGACAATATAAATCCAAGTATTGCTCAAATGTTACCACCACAAAGAGAATCCAGTGTTCCTCAAGCAGCTGACTTGGTAGAAGACTTCGGGCCACTGTCGAAATTGGCATTTGACTTGATGAGTAGACGACCTAGAATAAGGGAATCTGCTGATAGTTTTGCCTTTGATTTTGACAATCATCTTCAATCTGAAAGGACTCGAGATACGAAGGAGGGAGAGGTGGGACGTTTGCTGCATTACTTTCAAAGGCAGCACTTTGAAAACCCGTTGTTTTTTTATGCATTACAGGTTGATGCTGATGATAAAGTGAGTAACATATTTTGGGCTGATGATAATATGGTATCAGATTATGAACATTTTGGGGATGTGGTTTGTCTGGACTCAGTTTGCAGAACAGACACGAAGTGTCTGCCATTTGTACAGTTTATAGGCGTAAATCATCACAAACAGGTAGTTATTTTTTCTGCTGCACTTTTGTATGATGGCACTGTTCAATCTTTTGAGTGGCTATTTCAAACTTTCTTGGAGACAATGTCAGGGAAGAAACCAAAGGTCATTCATACCGATCAAGATCCAGCACTTGTTGAGGCAATAAATTCAGTCTTACCAGAAACAGACCATCGCATATGCACATGGCAAATGTGCCATAGTTCTCTCAAGCACCTTCACCACGTAGTAGAAGATACTGAGGCTTTTGTCAATGATTTTACGAGCTGTATATATGACCACAAAGATGAGGATGATTTTGTTCGTGCGTGGGGAGATATGCTGGATAATTATGGCCTTCGGAAGAATGACTGGTTAAAGTGGATGTTTAGGGAACGAGAAAAATGGGCTGTGGTATATAACAGGAATACTTTTTTTGTTGACATGAAAGGGTCACATCTGGTTGAGAGTTTATTCAATGATTTGAGAAAATACCTAAACTCTGACCCAGATGTGCTTCGTTTCTTTAGGCATTTTGAAAGGGTGGTAGATGAGCAACGTTGCAAAGAAATAGAAGCTAGTGTTGAAATGAACCGTTGTATTCCAAGACTGATGGGAAATGTGATTTTGTTAAGGCATGCAAGTAATGTTTACACTCCAAGAGCGTTTCAAGTATTTCAGCGAGGATACGAGAAGTCTTTAGATATTGTTGTTAATCAATGCAGTGAGAATGACTCATTGTTTGAGTACAAAACCAACATCTTTGGTAAAAGTACGGAGCACACAGTAACATTCGACTCTTCTGATGACATAGTCATTTGCAGTTGTAAGAAATTTGAGTCTGTTGGGTTTCTGTGTAGCCATGCTCTTAAAGTGCTTGATCACCGCAATATAAAGGTTCTCCCCTCCAAATATATCTTGAAGAGATGGACCAAAGATGCAAGGTTAGGGAGTGCAAGAGTGAGTGATGCCATCAGTGATAACCCTAAGCTTATTGTGGGAAGCCGTTACAAAATTCTGTGCCACCGGATTCTTATGTTACCAGCCAGGGCTTCTGAATCTGAGGAGGCTTTTCAGTTTGCTGTTAGACAACTTGATCAAGTGATGGAAGGTGTGGAGAAAATCTTAACATTAAGGCCTGAGGATGCTCAAGCTTTGACTTCAAGTAGCACGGCTGCAAATGTTTCTGATACTGAACATGCAAAGATTTTTCTAGATGAATTTGCAATCAATAATCAGGATGACAATAGAGTAAAAGGAGCCGAGGAAAAGGATGGAGCCGTTCTAGACAGAGGTCCATTAACAAATGTTAATGGCGATTACAATGCATCCATACAAAATACTGGTTCCTGCATTTCAAGTCCGCTATTGTACGCCTCACCTCAAGCTGCAGCGGAAAATGATATTATCCAGGGTTTGTACAATTTTGAAACGAACCAGGTGGTTCAGTGCATGCACGAACAAGATAGCCTGGTGATAAACCAGCAAACTGATCCTAACATTTTCTTTTCTAGCGAACATGATTCACCTGGTCATTCTCAACTACTGCAGGAACCTCTGATCAATGGTGTGTACCAAGAGCCCGTATCAAGCACCCCTGAAGTGAGGCAGGCAATGGATCTCGATGTCCAAACTCCATCGTCGTTCTTGGTTTTTGATGGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

843

Amino Acids

96.5

Weight (kDa)

5.66

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000325)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24391 FvH4_2g38071 FvH4_3g07131 FvH4_3g22481 FvH4_4g15809 FvH4_5g37230 FvH4_7g25201 FvH4_7g29092 FvH4_7g29093
malus_domestica MD01G1197600.v1.1 MD17G1186900.v1.1
prunus_persica Prupe.1G227400_v2.0.a1 Prupe.2G291000_v2.0.a1 Prupe.4G268600_v2.0.a1 Prupe.5G094100_v2.0.a1
pyrus_communis pycom01g20780 pycom17g19620
rosa_chinensis RchiOBHm_Chr1g0376821 RchiOBHm_Chr5g0061941 RchiOBHm_Chr5g0078991 RchiOBHm_Chr6g0276821 RchiOBHm_Chr7g0191321
rosa_laevigata RLG00000007883 RLG00000008804 RLG00000010632 RLG00000026567 RLG00000035244 RLG00000036018 RLG00000036909
rosa_multiflora Rmu_sc0001036.1_g000026 Rmu_sc0001556.1_g000021 Rmu_sc0001974.1_g000022 Rmu_sc0002113.1_g000006 Rmu_sc0002572.1_g000008 Rmu_sc0004808.1_g000011 Rmu_sc0005117.1_g000017 Rmu_sc0005319.1_g000016 Rmu_sc0008432.1_g000005 Rmu_sc0010475.1_g000001 Rmu_sc0015522.1_g000003 Rmu_ssc0000066.1_g000009 Rmu_ssc0000400.1_g000102 Rmu_ssc0000400.1_g000103
rosa_roxburghii Rroxscaffold_1G00071290 Rroxscaffold_2G00141280 Rroxscaffold_3G00233790 Rroxscaffold_4G00297530 Rroxscaffold_5G00368860 Rroxscaffold_5G00386220 Rroxscaffold_7G00159400
rosa_rugosa Rorug01G0146800.1 Rorug01G0162100.1 Rorug01G0162200.1 Rorug01G0398600 Rorug01G0398700 Rorug02G0344200 Rorug02G0344300 Rorug02G0344400 Rorug03G0046200 Rorug03G0153200 Rorug04G0182500 Rorug04G0182600 Rorug05G0257700 Rorug05G0386400 Rorug05G0386400 Rorug05G0386500 Rorug05G0460900.1 Rorug05G0467000 Rorug07G0110400
rosa_samantha Rh1AG183500 Rh1AG416000 Rh1BG023600 Rh1BG145400 Rh1BG145500 Rh1BG278800 Rh1BG375400 Rh1CG389200 Rh1DG166300 Rh1DG176900 Rh1DG309900 Rh1DG406000 Rh2BG439100 Rh2BG502200 Rh2CG314800 Rh2CG517400 Rh2CG532000 Rh2DG449100 Rh3BG109900 Rh3CG230400 Rh3CG246600 Rh3CG289400 Rh3CG289500 Rh3DG120600 Rh4AG144400 Rh4AG408200 Rh4BG419300 Rh4CG101900 Rh4CG151000 Rh4CG226900 Rh4DG235400 Rh5AG265900 Rh5AG375200 Rh5CG559600 Rh5DG218200 Rh6AG074500 Rh6BG291800 Rh6CG292800 Rh6DG284900
rosa_wichuraiana Rw0G013240 Rw1G036490 Rw5G024920 Rw5G030730 Rw5G033710 Rw6G033840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 559
AciI CCGC 3 cut(s) 1747, 2225, 2255
AclI AACGTT 1 cut(s) 1420
AclWI GGATC 3 cut(s) 1001, 2348, 2487
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 6 cut(s) 68, 152, 1030, 1694, 1875, 2081
AcuI CTGAAG 1 cut(s) 2481
AdeI CACNNNGTG 1 cut(s) 840
AfaI GTAC 7 cut(s) 249, 855, 1610, 1636, 2234, 2282, 2435
AjnI CCWGG 7 cut(s) 197, 255, 1907, 2271, 2298, 2331, 2389
AluBI AGCT 6 cut(s) 503, 1149, 1439, 1852, 2015, 2249
AluI AGCT 6 cut(s) 503, 1149, 1439, 1852, 2015, 2249
Alw21I GWGCWC 2 cut(s) 118, 1644
Alw26I GTCTC 1 cut(s) 956
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 3 cut(s) 1001, 2348, 2487
Ama87I CYCGRG 1 cut(s) 633
AoxI GGCC 4 cut(s) 253, 524, 1216, 1997
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 8 cut(s) 60, 500, 667, 685, 902, 2036, 2249, 2252
ApoI RAATTY 6 cut(s) 68, 152, 1030, 1694, 1875, 2081
ArsI GACNNNNNNTTYG 2 cut(s) 1586, 1618
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 3 cut(s) 524, 1791, 2156
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 5 cut(s) 1094, 1736, 2232, 2347, 2379
AsuII TTCGAA 2 cut(s) 156, 220
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 2 cut(s) 1791, 2156
AxyI CCTNAGG 1 cut(s) 2001
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 255
BanII GRGCYC 1 cut(s) 2445
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 2 cut(s) 118, 1644
BbvI GCAGC 8 cut(s) 72, 512, 654, 697, 889, 2023, 2236, 2264
BccI CCATC 9 cut(s) 911, 1062, 1468, 1782, 1841, 1960, 2129, 2506, 2513
BceAI ACGGC 4 cut(s) 36, 1851, 2049, 2126
BcgI CGANNNNNNTGC 4 cut(s) 826, 860, 2168, 2202
BciT130I CCWGG 7 cut(s) 199, 257, 1909, 2273, 2300, 2333, 2391
BciVI GTATCC 1 cut(s) 1541
BclI TGATCA 3 cut(s) 1741, 1957, 2421
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 6 cut(s) 285, 567, 1440, 2075, 2147, 2373
BfmI CTRYAG 2 cut(s) 2250, 2408
BfuI GTATCC 1 cut(s) 1541
BisI GCNGC 8 cut(s) 61, 501, 668, 686, 903, 2037, 2250, 2253
BlsI GCNGC 8 cut(s) 62, 502, 669, 687, 904, 2038, 2251, 2254
Bme1390I CCNGG 8 cut(s) 199, 257, 404, 1909, 2273, 2300, 2333, 2391
Bme18I GGWCC 2 cut(s) 1791, 2156
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 3 cut(s) 524, 1791, 2156
BmiI GGNNCC 4 cut(s) 2122, 2140, 2209, 2415
BmrFI CCNGG 8 cut(s) 199, 257, 404, 1909, 2273, 2300, 2333, 2391
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 6 cut(s) 307, 718, 1789, 1819, 1996, 2198
BmuI ACTGGG 1 cut(s) 238
BoxI GACNNNNGTC 1 cut(s) 2154
BpiI GAAGAC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 1580, 1612
Bpu10I CCTNAGC 1 cut(s) 1848
Bpu14I TTCGAA 2 cut(s) 156, 220
BpuEI CTTGAG 4 cut(s) 480, 1076, 1995, 2229
BpuMI CCSGG 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 1108
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 3 cut(s) 1908, 2124, 2272
BsaWI WCCGGW 1 cut(s) 1887
BsaXI ACNNNNNCTCC 2 cut(s) 953, 983
Bse1I ACTGG 5 cut(s) 233, 358, 486, 1238, 2209
Bse21I CCTNAGG 1 cut(s) 2001
Bse3DI GCAATG 1 cut(s) 2481
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 7 cut(s) 199, 257, 1909, 2273, 2300, 2333, 2391
BseDI CCNNGG 3 cut(s) 1908, 2124, 2272
BseGI GGATG 8 cut(s) 10, 805, 1180, 1251, 2011, 2107, 2140, 2189
BseJI GATNNNNATC 1 cut(s) 1005
BseMI GCAATG 1 cut(s) 2481
BseMII CTCAG 5 cut(s) 104, 831, 1113, 1914, 1992
BseNI ACTGG 5 cut(s) 233, 358, 486, 1238, 2209
BseRI GAGGAG 1 cut(s) 1940
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 8 cut(s) 72, 512, 654, 697, 889, 2023, 2236, 2264
BsgI GTGCAG 1 cut(s) 888
BshFI GGCC 4 cut(s) 255, 526, 1218, 1999
BsiHKAI GWGCWC 2 cut(s) 118, 1644
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 2 cut(s) 403, 1888
BslFI GGGAC 1 cut(s) 672
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 1 cut(s) 672
BsnI GGCC 4 cut(s) 255, 526, 1218, 1999
BsoBI CYCGRG 1 cut(s) 633
Bsp119I TTCGAA 2 cut(s) 156, 220
Bsp1286I GDGCHC 3 cut(s) 118, 1644, 2445
Bsp1407I TGTACA 2 cut(s) 853, 2280
Bsp143I GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
BspACI CCGC 3 cut(s) 1747, 2225, 2255
BspANI GGCC 4 cut(s) 255, 526, 1218, 1999
BspCNI CTCAG 5 cut(s) 103, 830, 1114, 1915, 1993
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 4 cut(s) 2122, 2140, 2209, 2415
BspMAI CTGCAG 2 cut(s) 2254, 2412
BspPI GGATC 3 cut(s) 1001, 2348, 2487
BspT104I TTCGAA 2 cut(s) 156, 220
BsrDI GCAATG 1 cut(s) 2481
BsrGI TGTACA 2 cut(s) 853, 2280
BsrI ACTGG 5 cut(s) 233, 358, 486, 1238, 2209
BssECI CCNNGG 3 cut(s) 1908, 2124, 2272
BssMI GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
Bst2UI CCWGG 7 cut(s) 199, 257, 1909, 2273, 2300, 2333, 2391
Bst4CI ACNGT 6 cut(s) 122, 531, 858, 925, 1457, 1648
Bst6I CTCTTC 2 cut(s) 1666, 1778
BstAUI TGTACA 2 cut(s) 853, 2280
BstBAI YACGTR 1 cut(s) 1108
BstBI TTCGAA 2 cut(s) 156, 220
BstC8I GCNNGC 3 cut(s) 1500, 2315, 2472
BstDEI CTNAG 6 cut(s) 90, 817, 1122, 1848, 1923, 2001
BstF5I GGATG 8 cut(s) 10, 805, 1180, 1251, 2011, 2107, 2140, 2189
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 7 cut(s) 1003, 1009, 1744, 1960, 2356, 2424, 2482
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
BstNI CCWGG 7 cut(s) 199, 257, 1909, 2273, 2300, 2333, 2391
BstNSI RCATGY 5 cut(s) 173, 371, 1502, 2063, 2317
BstPAI GACNNNNGTC 1 cut(s) 2154
BstSCI CCNGG 8 cut(s) 197, 255, 402, 1907, 2271, 2298, 2331, 2389
BstSFI CTRYAG 2 cut(s) 2250, 2408
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 8 cut(s) 72, 512, 654, 697, 889, 2023, 2236, 2264
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 2 cut(s) 1006, 2479
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 2 cut(s) 1006, 2479
Bsu36I CCTNAGG 1 cut(s) 2001
BsuI GTATCC 1 cut(s) 1541
BsuRI GGCC 4 cut(s) 255, 526, 1218, 1999
BtgZI GCGATG 1 cut(s) 1040
BtsCI GGATG 8 cut(s) 10, 805, 1180, 1251, 2011, 2107, 2140, 2189
BtsI GCAGTG 1 cut(s) 1591
BtsIMutI CAGTG 6 cut(s) 493, 527, 921, 1591, 1843, 2315
Cac8I GCNNGC 3 cut(s) 1500, 2315, 2472
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 524, 1791, 2156
CsiI ACCWGGT 2 cut(s) 2298, 2389
Csp6I GTAC 7 cut(s) 248, 854, 1609, 1635, 2233, 2281, 2434
CviQI GTAC 7 cut(s) 248, 854, 1609, 1635, 2233, 2281, 2434
DdeI CTNAG 6 cut(s) 90, 817, 1122, 1848, 1923, 2001
DpnI GATC 7 cut(s) 1002, 1008, 1743, 1959, 2355, 2423, 2481
DpnII GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
DraI TTTAAA 1 cut(s) 138
DraIII CACNNNGTG 1 cut(s) 840
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 1666, 1778
EarI CTCTTC 2 cut(s) 1666, 1778
Eco147I AGGCCT 1 cut(s) 1999
Eco24I GRGCYC 1 cut(s) 2445
Eco47I GGWCC 2 cut(s) 1791, 2156
Eco57I CTGAAG 1 cut(s) 2481
Eco81I CCTNAGG 1 cut(s) 2001
Eco88I CYCGRG 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 7 cut(s) 197, 255, 1907, 2271, 2298, 2331, 2389
EcoT22I ATGCAT 2 cut(s) 718, 2191
EcoT38I GRGCYC 1 cut(s) 2445
FaqI GGGAC 1 cut(s) 672
FauNDI CATATG 1 cut(s) 1061
FbaI TGATCA 3 cut(s) 1741, 1957, 2421
FblI GTMKAC 1 cut(s) 559
Fnu4HI GCNGC 8 cut(s) 61, 501, 668, 686, 903, 2037, 2250, 2253
FokI GGATG 8 cut(s) 17, 812, 1187, 1258, 2018, 2114, 2147, 2176
FriOI GRGCYC 1 cut(s) 2445
Fsp4HI GCNGC 8 cut(s) 61, 501, 668, 686, 903, 2037, 2250, 2253
FspBI CTAG 6 cut(s) 285, 567, 1440, 2075, 2147, 2373
GlaI GCGC 1 cut(s) 366
GluI GCNGC 8 cut(s) 61, 501, 668, 686, 903, 2037, 2250, 2253
HaeIII GGCC 4 cut(s) 255, 526, 1218, 1999
HapII CCGG 2 cut(s) 403, 1888
HhaI GCGC 1 cut(s) 367
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 2 cut(s) 386, 1303
HindII GTYRAC 2 cut(s) 386, 1303
HindIII AAGCTT 2 cut(s) 1850, 2013
HpaII CCGG 2 cut(s) 403, 1888
HphI GGTGA 5 cut(s) 1094, 1736, 2232, 2347, 2379
Hpy166II GTNNAC 7 cut(s) 116, 276, 386, 560, 1303, 1513, 2434
Hpy8I GTNNAC 7 cut(s) 116, 276, 386, 560, 1303, 1513, 2434
Hpy99I CGWCG 2 cut(s) 339, 2506
HpyAV CCTTC 5 cut(s) 284, 637, 1109, 1229, 1964
HpyCH4III ACNGT 6 cut(s) 122, 531, 858, 925, 1457, 1648
HpyCH4IV ACGT 4 cut(s) 334, 661, 1107, 1420
HpyF3I CTNAG 6 cut(s) 90, 817, 1122, 1848, 1923, 2001
HpySE526I ACGT 4 cut(s) 334, 661, 1107, 1420
HspAI GCGC 1 cut(s) 365
Ksp22I TGATCA 3 cut(s) 1741, 1957, 2421
Kzo9I GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
LmnI GCTCC 3 cut(s) 1639, 2120, 2138
Lsp1109I GCAGC 8 cut(s) 72, 512, 654, 697, 889, 2023, 2236, 2264
LweI GCATC 6 cut(s) 307, 718, 1789, 1819, 1996, 2198
MabI ACCWGGT 2 cut(s) 2298, 2389
MaeI CTAG 6 cut(s) 285, 567, 1440, 2075, 2147, 2373
MaeII ACGT 4 cut(s) 334, 661, 1107, 1420
MaeIII GTNAC 7 cut(s) 406, 465, 746, 1314, 1648, 1868, 1899
MalI GATC 7 cut(s) 1002, 1008, 1743, 1959, 2355, 2423, 2481
MboI GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
MboII GAAGA 9 cut(s) 96, 431, 527, 608, 988, 1127, 1237, 1653, 1795
MflI RGATCY 2 cut(s) 1006, 2479
MhlI GDGCHC 3 cut(s) 118, 1644, 2445
MlsI TGGCCA 1 cut(s) 255
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 5 cut(s) 625, 809, 1589, 1652, 1709
MmeI TCCRAC 1 cut(s) 12
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 2 cut(s) 718, 2191
MscI TGGCCA 1 cut(s) 255
MslI CAYNNNNRTG 2 cut(s) 432, 1167
Msp20I TGGCCA 1 cut(s) 255
MspA1I CMGCKG 2 cut(s) 503, 2255
MspI CCGG 2 cut(s) 403, 1888
MspR9I CCNGG 8 cut(s) 199, 257, 404, 1909, 2273, 2300, 2333, 2391
MvaI CCWGG 7 cut(s) 199, 257, 1909, 2273, 2300, 2333, 2391
NciI CCSGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 1061
NdeII GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
NlaIV GGNNCC 4 cut(s) 2122, 2140, 2209, 2415
NmeAIII GCCGAG 1 cut(s) 2149
NmuCI GTSAC 1 cut(s) 1314
NsiI ATGCAT 2 cut(s) 718, 2191
NspI RCATGY 5 cut(s) 173, 371, 1502, 2063, 2317
NspV TTCGAA 2 cut(s) 156, 220
PaeI GCATGC 2 cut(s) 1502, 2317
PaeR7I CTCGAG 1 cut(s) 633
PagI TCATGA 1 cut(s) 433
PceI AGGCCT 1 cut(s) 1999
PfeI GAWTC 7 cut(s) 128, 158, 482, 578, 1891, 1919, 2384
PflFI GACNNNGTC 3 cut(s) 230, 967, 1673
PkrI GCNGC 8 cut(s) 62, 502, 669, 687, 904, 2038, 2251, 2254
PleI GAGTC 5 cut(s) 625, 809, 1589, 1652, 1708
PpsI GAGTC 5 cut(s) 625, 809, 1589, 1652, 1708
Ppu21I YACGTR 1 cut(s) 1108
PshAI GACNNNNGTC 1 cut(s) 2154
Psp1406I AACGTT 1 cut(s) 1420
Psp6I CCWGG 7 cut(s) 197, 255, 1907, 2271, 2298, 2331, 2389
PspGI CCWGG 7 cut(s) 197, 255, 1907, 2271, 2298, 2331, 2389
PspN4I GGNNCC 4 cut(s) 2122, 2140, 2209, 2415
PspPI GGNCC 3 cut(s) 524, 1791, 2156
PstI CTGCAG 2 cut(s) 2254, 2412
PsuI RGATCY 2 cut(s) 1006, 2479
PsyI GACNNNGTC 3 cut(s) 230, 967, 1673
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 7 cut(s) 249, 855, 1610, 1636, 2234, 2282, 2435
RsaNI GTAC 7 cut(s) 248, 854, 1609, 1635, 2233, 2281, 2434
RseI CAYNNNNRTG 2 cut(s) 432, 1167
SatI GCNGC 8 cut(s) 61, 501, 668, 686, 903, 2037, 2250, 2253
Sau3AI GATC 7 cut(s) 1000, 1006, 1741, 1957, 2353, 2421, 2479
Sau96I GGNCC 3 cut(s) 524, 1791, 2156
SchI GAGTC 5 cut(s) 625, 809, 1589, 1652, 1709
ScrFI CCNGG 8 cut(s) 199, 257, 404, 1909, 2273, 2300, 2333, 2391
SduI GDGCHC 3 cut(s) 118, 1644, 2445
SexAI ACCWGGT 2 cut(s) 2298, 2389
SfaNI GCATC 6 cut(s) 307, 718, 1789, 1819, 1996, 2198
SfcI CTRYAG 2 cut(s) 2250, 2408
Sfr274I CTCGAG 1 cut(s) 633
SfuI TTCGAA 2 cut(s) 156, 220
SinI GGWCC 2 cut(s) 1791, 2156
SlaI CTCGAG 1 cut(s) 633
SmiMI CAYNNNNRTG 2 cut(s) 432, 1167
SmlI CTYRAG 5 cut(s) 495, 633, 1091, 2010, 2244
SmoI CTYRAG 5 cut(s) 495, 633, 1091, 2010, 2244
SphI GCATGC 2 cut(s) 1502, 2317
SseBI AGGCCT 1 cut(s) 1999
SsiI CCGC 3 cut(s) 1747, 2225, 2255
SspI AATATT 1 cut(s) 100
SspMI CTAG 6 cut(s) 285, 567, 1440, 2075, 2147, 2373
StuI AGGCCT 1 cut(s) 1999
StyD4I CCNGG 8 cut(s) 197, 255, 402, 1907, 2271, 2298, 2331, 2389
TaaI ACNGT 6 cut(s) 122, 531, 858, 925, 1457, 1648
TaiI ACGT 4 cut(s) 337, 664, 1110, 1423
TaqI TCGA 8 cut(s) 156, 220, 266, 298, 533, 634, 1656, 2484
TatI WGTACW 3 cut(s) 853, 1608, 2280
TfiI GAWTC 7 cut(s) 128, 158, 482, 578, 1891, 1919, 2384
TscAI CASTG 6 cut(s) 493, 534, 928, 1591, 1843, 2315
TseFI GTSAC 1 cut(s) 1314
TseI GCWGC 8 cut(s) 60, 500, 667, 685, 902, 2036, 2249, 2252
Tsp45I GTSAC 1 cut(s) 1314
TspDTI ATGAA 6 cut(s) 180, 801, 983, 1322, 1466, 2094
TspGWI ACGGA 1 cut(s) 1652
TspRI CASTG 6 cut(s) 493, 534, 928, 1591, 1843, 2315
Tth111I GACNNNGTC 3 cut(s) 230, 967, 1673
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 1791, 2156
XapI RAATTY 6 cut(s) 68, 152, 1030, 1694, 1875, 2081
XbaI TCTAGA 3 cut(s) 284, 2074, 2146
XceI RCATGY 5 cut(s) 173, 371, 1502, 2063, 2317
XhoI CTCGAG 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 559
XspI CTAG 6 cut(s) 285, 567, 1440, 2075, 2147, 2373
Zsp2I ATGCAT 2 cut(s) 718, 2191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.