DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 55 |
| AciI | CCGC | 2 cut(s) | 5, 8 |
| AclWI | GGATC | 2 cut(s) | 68, 81 |
| AcuI | CTGAAG | 1 cut(s) | 91 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 232 |
| AgsI | TTSAA | 1 cut(s) | 133 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 185, 217 |
| AluBI | AGCT | 2 cut(s) | 111, 299 |
| AluI | AGCT | 2 cut(s) | 111, 299 |
| AlwI | GGATC | 2 cut(s) | 68, 81 |
| AoxI | GGCC | 1 cut(s) | 84 |
| AspLEI | GCGC | 1 cut(s) | 98 |
| AspS9I | GGNCC | 1 cut(s) | 84 |
| AsuC2I | CCSGG | 1 cut(s) | 83 |
| AsuHPI | GGTGA | 3 cut(s) | 133, 215, 217 |
| BaeGI | GKGCMC | 1 cut(s) | 60 |
| BamHI | GGATCC | 1 cut(s) | 73 |
| BanI | GGYRCC | 1 cut(s) | 55 |
| BanII | GRGCYC | 1 cut(s) | 288 |
| BccI | CCATC | 1 cut(s) | 181 |
| BcnI | CCSGG | 1 cut(s) | 83 |
| BfmI | CTRYAG | 1 cut(s) | 49 |
| BisI | GCNGC | 1 cut(s) | 6 |
| BlsI | GCNGC | 1 cut(s) | 7 |
| Bme1390I | CCNGG | 1 cut(s) | 83 |
| BmgT120I | GGNCC | 1 cut(s) | 84 |
| BmiI | GGNNCC | 2 cut(s) | 57, 75 |
| BmrFI | CCNGG | 1 cut(s) | 83 |
| Bpu10I | CCTNAGC | 1 cut(s) | 287 |
| BpuMI | CCSGG | 1 cut(s) | 83 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 232 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 232 |
| BseMII | CTCAG | 1 cut(s) | 301 |
| BseSI | GKGCMC | 1 cut(s) | 60 |
| BshFI | GGCC | 1 cut(s) | 86 |
| BshNI | GGYRCC | 1 cut(s) | 55 |
| BsiSI | CCGG | 2 cut(s) | 24, 82 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 232 |
| BsnI | GGCC | 1 cut(s) | 86 |
| Bsp1286I | GDGCHC | 2 cut(s) | 60, 288 |
| Bsp143I | GATC | 2 cut(s) | 73, 123 |
| BspACI | CCGC | 2 cut(s) | 5, 8 |
| BspANI | GGCC | 1 cut(s) | 86 |
| BspCNI | CTCAG | 1 cut(s) | 300 |
| BspLI | GGNNCC | 2 cut(s) | 57, 75 |
| BspPI | GGATC | 2 cut(s) | 68, 81 |
| BspT107I | GGYRCC | 1 cut(s) | 55 |
| BssMI | GATC | 2 cut(s) | 73, 123 |
| BstDEI | CTNAG | 2 cut(s) | 61, 287 |
| BstHHI | GCGC | 1 cut(s) | 98 |
| BstKTI | GATC | 2 cut(s) | 76, 126 |
| BstMBI | GATC | 2 cut(s) | 73, 123 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 283 |
| BstSCI | CCNGG | 1 cut(s) | 81 |
| BstSFI | CTRYAG | 1 cut(s) | 49 |
| BstSLI | GKGCMC | 1 cut(s) | 60 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| BsuRI | GGCC | 1 cut(s) | 86 |
| BtsIMutI | CAGTG | 1 cut(s) | 150 |
| CfoI | GCGC | 1 cut(s) | 98 |
| Cfr13I | GGNCC | 1 cut(s) | 84 |
| CviJI | RGCY | 5 cut(s) | 86, 111, 272, 286, 299 |
| CviKI_1 | RGCY | 5 cut(s) | 86, 111, 272, 286, 299 |
| DdeI | CTNAG | 2 cut(s) | 61, 287 |
| DpnI | GATC | 2 cut(s) | 75, 125 |
| DpnII | GATC | 2 cut(s) | 73, 123 |
| Eco24I | GRGCYC | 1 cut(s) | 288 |
| Eco57I | CTGAAG | 1 cut(s) | 91 |
| EcoT38I | GRGCYC | 1 cut(s) | 288 |
| FaiI | YATR | 6 cut(s) | 36, 162, 179, 231, 266, 275 |
| Fnu4HI | GCNGC | 1 cut(s) | 6 |
| FriOI | GRGCYC | 1 cut(s) | 288 |
| Fsp4HI | GCNGC | 1 cut(s) | 6 |
| GlaI | GCGC | 1 cut(s) | 97 |
| GluI | GCNGC | 1 cut(s) | 6 |
| HaeIII | GGCC | 1 cut(s) | 86 |
| HapII | CCGG | 2 cut(s) | 24, 82 |
| HhaI | GCGC | 1 cut(s) | 98 |
| Hin6I | GCGC | 1 cut(s) | 96 |
| HinP1I | GCGC | 1 cut(s) | 96 |
| HinfI | GANTC | 4 cut(s) | 12, 20, 142, 255 |
| HpaII | CCGG | 2 cut(s) | 24, 82 |
| HphI | GGTGA | 3 cut(s) | 133, 215, 217 |
| Hpy188III | TCNNGA | 1 cut(s) | 294 |
| Hpy99I | CGWCG | 2 cut(s) | 71, 118 |
| HpyAV | CCTTC | 3 cut(s) | 97, 109, 139 |
| HpyCH4V | TGCA | 1 cut(s) | 34 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 283 |
| HpyF3I | CTNAG | 2 cut(s) | 61, 287 |
| HspAI | GCGC | 1 cut(s) | 96 |
| Kzo9I | GATC | 2 cut(s) | 73, 123 |
| LmnI | GCTCC | 1 cut(s) | 296 |
| LpnPI | CCDG | 7 cut(s) | 37, 95, 104, 106, 195, 274, 279 |
| MaeIII | GTNAC | 2 cut(s) | 148, 205 |
| MalI | GATC | 2 cut(s) | 75, 125 |
| MboI | GATC | 2 cut(s) | 73, 123 |
| MboII | GAAGA | 2 cut(s) | 94, 97 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MhlI | GDGCHC | 2 cut(s) | 60, 288 |
| MluCI | AATT | 1 cut(s) | 133 |
| MlyI | GAGTC | 1 cut(s) | 21 |
| MnlI | CCTC | 2 cut(s) | 87, 237 |
| MspI | CCGG | 2 cut(s) | 24, 82 |
| MspR9I | CCNGG | 1 cut(s) | 83 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 283 |
| NciI | CCSGG | 1 cut(s) | 83 |
| NdeII | GATC | 2 cut(s) | 73, 123 |
| NlaIV | GGNNCC | 2 cut(s) | 57, 75 |
| NmuCI | GTSAC | 2 cut(s) | 148, 205 |
| PfeI | GAWTC | 3 cut(s) | 20, 142, 255 |
| PkrI | GCNGC | 1 cut(s) | 7 |
| PleI | GAGTC | 1 cut(s) | 20 |
| PpsI | GAGTC | 1 cut(s) | 20 |
| PspN4I | GGNNCC | 2 cut(s) | 57, 75 |
| PspPI | GGNCC | 1 cut(s) | 84 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| SatI | GCNGC | 1 cut(s) | 6 |
| Sau3AI | GATC | 2 cut(s) | 73, 123 |
| Sau96I | GGNCC | 1 cut(s) | 84 |
| SchI | GAGTC | 1 cut(s) | 21 |
| ScrFI | CCNGG | 1 cut(s) | 83 |
| SduI | GDGCHC | 2 cut(s) | 60, 288 |
| SetI | ASST | 5 cut(s) | 113, 161, 214, 293, 301 |
| SfcI | CTRYAG | 1 cut(s) | 49 |
| Sse9I | AATT | 1 cut(s) | 133 |
| SsiI | CCGC | 2 cut(s) | 5, 8 |
| StyD4I | CCNGG | 1 cut(s) | 81 |
| TasI | AATT | 1 cut(s) | 133 |
| TauI | GCSGC | 1 cut(s) | 8 |
| TfiI | GAWTC | 3 cut(s) | 20, 142, 255 |
| TscAI | CASTG | 1 cut(s) | 157 |
| TseFI | GTSAC | 2 cut(s) | 148, 205 |
| Tsp45I | GTSAC | 2 cut(s) | 148, 205 |
| TspDTI | ATGAA | 1 cut(s) | 186 |
| TspRI | CASTG | 1 cut(s) | 157 |