AT4G20020

mitochondrial RNA modification

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005120
Physical Location & Seq
Reverse (-)
10844131 .. 10846163
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20020.1

Sequence Viewer

Length: 1260 bp
ATGGCTATGATATCTCACCGTCTCCGACGAGCTTTACTCACTGCGACTTCCTATGTTAACCGATCAATTAGCTCTTCGATCACACCAGCGTCTGATTTCCCTTCTGTGTCGGCGGCGGTGTTAAAGAGATCCGTTATAGGGCGTTCGACGGAAGTAGCTACTCGAGCACCGGCGAGACTGTTTTCAACGAGGCAGTACAAGCTTTACAAAGAAGGTGATGAGATCACGGAAGACACTGTGTTGTTCGAAGGATGTGATTATAATCACTGGCTTATCACTATGGATTTCTCCAAAGAGGAAACTCCTAAGTCTCCAGAAGAAATGGTCGCTGCTTATGAAGAAACTTGCGCCCAAGGACTTGGCATCAGCGTGGAAGAGGCTAAGCAAAGGATGTATGCTTGTAGCACAACTACTTACCAAGGATTTCAAGCAATCATGACTGAACAAGAATCAGAAAAGTTCAAGGATCTACCCGGAGTGGTTTTCATCTTGCCAGATTCCTACATTGATCCCCAGAACAAAGAGTATGGAGGAGATAAATACGAGAATGGAGTGATAACACACAGGCCACCACCGATTCAGAGTGGCAGAGCAAGACCTCGTCCTAGATTTGACCGTTCAGGAGGAGGAAGTGGTGGTCCCCAAAACTTCCAAAGAAACACACAGTATGGTCAGCAACCACCAATGCAAGGTGGTGGAGGGAGCTATGGTCCTCAACAGGGCTATGCCACTCCAGGACAAGGTCAGGGAACTCAAGCGCCTCCACCATTTCAAGGAGGTTATAACCAAGGCCCGAGATCTCCACCACCTCCATATCAGGCAGGTTACAATCAAGGCCAGGGATCTCCAGTGCCTCCATATCAGGCAGGTTACAATCAAGTCCAGGGATCTCCAGTGCCTCCATACCAAGGAACACAAAGCAGTTACGGTCAAGGTGGATCTGGGAACTATAGTCAAGGGCCACAAGGTGGTTACAACCAAGGAGGACCTAGGAATTACAATCCACAGGGAGCTGGAAACTTTGGTCCTGCATCTGGAGCTGGAAACTTAGGTCCTGCACCTGGAGCTGGAAATCCTGGCTATGGTCAGGGGTATTCTGGACCAGGACAAGAGCAGAATCAGACATTCCCACAGGCAGATCAGAGGAATCGAGACTGGAACAACAATAATCCAGCAGGCCAGCCCGGGTCTGACCAAGTAAGATCTCGATCAATAATGAATCTTGCATCTTTCTTTTTTGATATCCTCATCAGGCATTAA

Protein Analysis

419

Amino Acids

45.16

Weight (kDa)

7.65

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 87 - 181 3e-34 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 261, 783
Acc36I ACCTGC 2 cut(s) 812, 857
AccB7I CCANNNNNTGG 1 cut(s) 968
AciI CCGC 2 cut(s) 113, 116
AclWI GGATC 6 cut(s) 123, 474, 503, 850, 895, 946
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 1 cut(s) 197
AgsI TTSAA 4 cut(s) 186, 428, 463, 773
AjnI CCWGG 6 cut(s) 733, 837, 882, 1060, 1075, 1102
AluBI AGCT 8 cut(s) 32, 72, 158, 202, 705, 1013, 1040, 1067
AluI AGCT 8 cut(s) 32, 72, 158, 202, 705, 1013, 1040, 1067
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 4 cut(s) 26, 169, 315, 1146
AlwI GGATC 6 cut(s) 123, 474, 503, 850, 895, 946
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 3 cut(s) 162, 793, 1184
AoxI GGCC 5 cut(s) 566, 790, 835, 959, 1177
ApeKI GCWGC 1 cut(s) 329
AspA2I CCTAGG 1 cut(s) 989
AspLEI GCGC 2 cut(s) 350, 760
AspS9I GGNCC 8 cut(s) 638, 710, 791, 959, 986, 1025, 1052, 1100
AsuC2I CCSGG 3 cut(s) 474, 1185, 1186
AsuHPI GGTGA 2 cut(s) 8, 227
AsuII TTCGAA 1 cut(s) 246
AvaI CYCGRG 3 cut(s) 162, 793, 1184
AvaII GGWCC 6 cut(s) 638, 710, 986, 1025, 1052, 1100
AvrII CCTAGG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 316
BciT130I CCWGG 6 cut(s) 735, 839, 884, 1062, 1077, 1104
BcnI CCSGG 3 cut(s) 474, 1185, 1186
BcoDI GTCTC 4 cut(s) 26, 169, 315, 1146
BfaI CTAG 2 cut(s) 606, 990
BfmI CTRYAG 1 cut(s) 949
BfoI RGCGCY 1 cut(s) 761
BfuAI ACCTGC 2 cut(s) 812, 857
BglII AGATCT 2 cut(s) 797, 1202
BisI GCNGC 2 cut(s) 114, 330
BlnI CCTAGG 1 cut(s) 989
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 2 cut(s) 115, 331
Bme1390I CCNGG 9 cut(s) 474, 735, 839, 884, 1062, 1077, 1104, 1185, 1186
Bme18I GGWCC 6 cut(s) 638, 710, 986, 1025, 1052, 1100
BmeT110I CYCGRG 3 cut(s) 162, 793, 1184
BmgT120I GGNCC 8 cut(s) 638, 710, 791, 959, 986, 1025, 1052, 1100
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 9 cut(s) 474, 735, 839, 884, 1062, 1077, 1104, 1185, 1186
BmsI GCATC 3 cut(s) 372, 1040, 1235
BpiI GAAGAC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 21, 53
BpmI CTGGAG 6 cut(s) 297, 717, 831, 876, 1056, 1083
Bpu1102I GCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 738
BpuMI CCSGG 3 cut(s) 474, 1185, 1186
BsaBI GATNNNNATC 2 cut(s) 261, 1207
BsaJI CCNNGG 9 cut(s) 352, 418, 787, 838, 883, 907, 979, 989, 1184
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 4 cut(s) 272, 848, 893, 1160
Bse8I GATNNNNATC 2 cut(s) 261, 1207
BseBI CCWGG 6 cut(s) 735, 839, 884, 1062, 1077, 1104
BseDI CCNNGG 9 cut(s) 352, 418, 787, 838, 883, 907, 979, 989, 1184
BseGI GGATG 2 cut(s) 257, 396
BseJI GATNNNNATC 2 cut(s) 261, 1207
BseNI ACTGG 4 cut(s) 272, 848, 893, 1160
BseRI GAGGAG 2 cut(s) 546, 639
BseXI GCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 1041
BshFI GGCC 5 cut(s) 568, 792, 837, 961, 1179
BsiHKAI GWGCWC 1 cut(s) 169
BsiHKCI CYCGRG 3 cut(s) 162, 793, 1184
BsiSI CCGG 3 cut(s) 170, 474, 1185
BslFI GGGAC 1 cut(s) 624
BsmAI GTCTC 4 cut(s) 26, 169, 315, 1146
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 5 cut(s) 568, 792, 837, 961, 1179
BsoBI CYCGRG 3 cut(s) 162, 793, 1184
Bsp119I TTCGAA 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 169
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 2 cut(s) 113, 116
BspANI GGCC 5 cut(s) 568, 792, 837, 961, 1179
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 640
BspMI ACCTGC 2 cut(s) 812, 857
BspPI GGATC 6 cut(s) 123, 474, 503, 850, 895, 946
BspQI GCTCTTC 1 cut(s) 79
BspT104I TTCGAA 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 4 cut(s) 272, 848, 893, 1160
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 9 cut(s) 352, 418, 787, 838, 883, 907, 979, 989, 1184
BssT1I CCWWGG 6 cut(s) 352, 418, 787, 907, 979, 989
Bst2UI CCWGG 6 cut(s) 735, 839, 884, 1062, 1077, 1104
Bst4CI ACNGT 6 cut(s) 20, 180, 238, 617, 666, 929
Bst6I CTCTTC 2 cut(s) 79, 369
BstBI TTCGAA 1 cut(s) 246
BstC8I GCNNGC 2 cut(s) 1177, 1181
BstDEI CTNAG 3 cut(s) 306, 381, 1048
BstF5I GGATG 2 cut(s) 257, 396
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 2 cut(s) 350, 760
BstMAI GTCTC 4 cut(s) 26, 169, 315, 1146
BstMWI GCNNNNNNNGC 4 cut(s) 164, 199, 1037, 1064
BstNI CCWGG 6 cut(s) 735, 839, 884, 1062, 1077, 1104
BstSCI CCNGG 9 cut(s) 472, 733, 837, 882, 1060, 1075, 1102, 1183, 1184
BstSFI CTRYAG 1 cut(s) 949
BstV1I GCAGC 1 cut(s) 316
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 7 cut(s) 128, 466, 797, 842, 887, 938, 1202
BstXI CCANNNNNNTGG 1 cut(s) 359
BstYI RGATCY 7 cut(s) 128, 466, 797, 842, 887, 938, 1202
BsuRI GGCC 5 cut(s) 568, 792, 837, 961, 1179
BtsCI GGATG 2 cut(s) 257, 396
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 5 cut(s) 39, 234, 265, 855, 900
BveI ACCTGC 2 cut(s) 812, 857
Cac8I GCNNGC 2 cut(s) 1177, 1181
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 435
CfoI GCGC 2 cut(s) 350, 760
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 8 cut(s) 638, 710, 791, 959, 986, 1025, 1052, 1100
Cfr9I CCCGGG 1 cut(s) 1184
CseI GACGC 1 cut(s) 78
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 436
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 3 cut(s) 306, 381, 1048
DraIII CACNNNGTG 1 cut(s) 968
Eam1104I CTCTTC 2 cut(s) 79, 369
EarI CTCTTC 2 cut(s) 79, 369
Eco130I CCWWGG 6 cut(s) 352, 418, 787, 907, 979, 989
Eco32I GATATC 2 cut(s) 12, 1243
Eco47I GGWCC 6 cut(s) 638, 710, 986, 1025, 1052, 1100
Eco88I CYCGRG 3 cut(s) 162, 793, 1184
EcoO109I RGGNCCY 2 cut(s) 986, 1052
EcoRII CCWGG 6 cut(s) 733, 837, 882, 1060, 1075, 1102
EcoRV GATATC 2 cut(s) 12, 1243
EcoT14I CCWWGG 6 cut(s) 352, 418, 787, 907, 979, 989
ErhI CCWWGG 6 cut(s) 352, 418, 787, 907, 979, 989
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 1 cut(s) 439
FaqI GGGAC 1 cut(s) 624
FatI CATG 1 cut(s) 435
Fnu4HI GCNGC 2 cut(s) 114, 330
FokI GGATG 2 cut(s) 264, 403
Fsp4HI GCNGC 2 cut(s) 114, 330
FspBI CTAG 2 cut(s) 606, 990
GlaI GCGC 2 cut(s) 349, 759
GluI GCNGC 2 cut(s) 114, 330
GsuI CTGGAG 6 cut(s) 297, 717, 831, 876, 1056, 1083
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 5 cut(s) 568, 792, 837, 961, 1179
HapII CCGG 3 cut(s) 170, 474, 1185
HgaI GACGC 1 cut(s) 78
HhaI GCGC 2 cut(s) 350, 760
Hin1II CATG 1 cut(s) 439
Hin6I GCGC 2 cut(s) 348, 758
HinP1I GCGC 2 cut(s) 348, 758
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 6 cut(s) 449, 497, 577, 1117, 1147, 1219
HpaI GTTAAC 1 cut(s) 58
HpaII CCGG 3 cut(s) 170, 474, 1185
HphI GGTGA 2 cut(s) 8, 227
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 7 cut(s) 26, 94, 454, 582, 1122, 1143, 1192
Hpy188III TCNNGA 7 cut(s) 314, 436, 621, 1035, 1098, 1151, 1206
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 2 cut(s) 30, 151
HpyAV CCTTC 3 cut(s) 111, 206, 242
HpyCH4III ACNGT 6 cut(s) 20, 180, 238, 617, 666, 929
HpyCH4V TGCA 4 cut(s) 688, 1031, 1058, 1226
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 199, 1037, 1064
HpyF3I CTNAG 3 cut(s) 306, 381, 1048
Hsp92II CATG 1 cut(s) 439
HspAI GCGC 2 cut(s) 348, 758
KspAI GTTAAC 1 cut(s) 58
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 4 cut(s) 702, 1010, 1037, 1064
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 3 cut(s) 372, 1040, 1235
MaeI CTAG 2 cut(s) 606, 990
MaeIII GTNAC 4 cut(s) 824, 869, 923, 971
MboII GAAGA 5 cut(s) 66, 242, 329, 350, 386
MflI RGATCY 7 cut(s) 128, 466, 797, 842, 887, 938, 1202
MhlI GDGCHC 1 cut(s) 169
MluCI AATT 2 cut(s) 66, 994
MmeI TCCRAC 1 cut(s) 49
MseI TTAA 3 cut(s) 57, 122, 1258
MslI CAYNNNNRTG 1 cut(s) 368
MspI CCGG 3 cut(s) 170, 474, 1185
MspR9I CCNGG 9 cut(s) 474, 735, 839, 884, 1062, 1077, 1104, 1185, 1186
MvaI CCWGG 6 cut(s) 735, 839, 884, 1062, 1077, 1104
MwoI GCNNNNNNNGC 4 cut(s) 164, 199, 1037, 1064
NciI CCSGG 3 cut(s) 474, 1185, 1186
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 1 cut(s) 640
NspV TTCGAA 1 cut(s) 246
PaeR7I CTCGAG 1 cut(s) 162
PagI TCATGA 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 79
PfeI GAWTC 6 cut(s) 449, 497, 577, 1117, 1147, 1219
PflFI GACNNNGTC 2 cut(s) 600, 741
PflMI CCANNNNNTGG 1 cut(s) 968
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 2 cut(s) 115, 331
PpuMI RGGWCCY 2 cut(s) 986, 1052
PsiI TTATAA 2 cut(s) 261, 783
Psp5II RGGWCCY 2 cut(s) 986, 1052
Psp6I CCWGG 6 cut(s) 733, 837, 882, 1060, 1075, 1102
PspGI CCWGG 6 cut(s) 733, 837, 882, 1060, 1075, 1102
PspN4I GGNNCC 1 cut(s) 640
PspPI GGNCC 8 cut(s) 638, 710, 791, 959, 986, 1025, 1052, 1100
PspPPI RGGWCCY 2 cut(s) 986, 1052
PspXI VCTCGAGB 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 7 cut(s) 128, 466, 797, 842, 887, 938, 1202
PsyI GACNNNGTC 2 cut(s) 600, 741
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 3 cut(s) 57, 122, 1258
SatI GCNGC 2 cut(s) 114, 330
Sau96I GGNCC 8 cut(s) 638, 710, 791, 959, 986, 1025, 1052, 1100
ScrFI CCNGG 9 cut(s) 474, 735, 839, 884, 1062, 1077, 1104, 1185, 1186
SduI GDGCHC 1 cut(s) 169
SfaNI GCATC 3 cut(s) 372, 1040, 1235
SfcI CTRYAG 1 cut(s) 949
Sfr274I CTCGAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 246
SgrAI CRCCGGYG 1 cut(s) 169
SinI GGWCC 6 cut(s) 638, 710, 986, 1025, 1052, 1100
SlaI CTCGAG 1 cut(s) 162
SmaI CCCGGG 1 cut(s) 1186
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 162, 753
SmoI CTYRAG 2 cut(s) 162, 753
Sse9I AATT 2 cut(s) 66, 994
SsiI CCGC 2 cut(s) 113, 116
SspMI CTAG 2 cut(s) 606, 990
StyD4I CCNGG 9 cut(s) 472, 733, 837, 882, 1060, 1075, 1102, 1183, 1184
StyI CCWWGG 6 cut(s) 352, 418, 787, 907, 979, 989
TaaI ACNGT 6 cut(s) 20, 180, 238, 617, 666, 929
TaqI TCGA 6 cut(s) 77, 146, 163, 246, 1150, 1207
TasI AATT 2 cut(s) 66, 994
TatI WGTACW 1 cut(s) 195
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 6 cut(s) 449, 497, 577, 1117, 1147, 1219
Tru1I TTAA 3 cut(s) 57, 122, 1258
Tru9I TTAA 3 cut(s) 57, 122, 1258
TscAI CASTG 5 cut(s) 46, 241, 272, 855, 900
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 351, 475, 1232
TspGWI ACGGA 3 cut(s) 121, 164, 242
TspMI CCCGGG 1 cut(s) 1184
TspRI CASTG 5 cut(s) 46, 241, 272, 855, 900
Tth111I GACNNNGTC 2 cut(s) 600, 741
Van91I CCANNNNNTGG 1 cut(s) 968
VpaK11BI GGWCC 6 cut(s) 638, 710, 986, 1025, 1052, 1100
XhoI CTCGAG 1 cut(s) 162
XmaI CCCGGG 1 cut(s) 1184
XmaJI CCTAGG 1 cut(s) 989
XspI CTAG 2 cut(s) 606, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.