pycom08g20520

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005120
Physical Location & Seq
Forward (+)
20296164 .. 20297786
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20520.1

Sequence Viewer

Length: 630 bp
ATGGCGCTGAGCTCTCTCCGCCTCCGCCGAACCATAAGCGGTCTCTCCACCCTCCATCGCTCCCTCTCTGCAACCTCCGCACTCTCAGCTCCACACTCCTTTTCCTCTCCTCTGCTTCCCGCCTCCACGTCCGAAAAGTTCCCTGCGCTGCCGCCACAGTCCTGGAGGCTCCTCCTCCAGTCTCGGACGTTCAGGTCGTCGCCGTTCTCTTCCGCGCGACCGAATTCGTACGGCAACAACCAAAATGACGAGATTGGCCCGGATACGATACTGTTCGAAGGGTGTGACTACAACCACTGGCTGATCGTCATGGATAACCCGAGGGACAACAAGCCTCCGGCTGAGGAGATGATCAGGAATTATGAGGAGACTTGTGCTAAAGGCTTGAACATCAGTGTGGAAGAGGCAAAAAAGAAGATATATGCTTGTAGCACTACAACATATACAGGCTTTCAGGTTGTGATGTCTGAGGAAGAGTCACAGAAGTTCGAAGGAGACAAATACATTAATGGTACAATCATACCAAGACCACCCCCAGTTCAGTATGGAAGGCAGCAGGGTGGAAGATTCCGTGATAATAACAGGAATCGTGACCAACAAAGATATCGATCAGCAACGACCATCGTATAA

Protein Analysis

210

Amino Acids

23.6

Weight (kDa)

9.19

Isoelectric Point (pI)

68.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 97 - 164 2.3e-24 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
AccII CGCG 2 cut(s) 215, 217
AciI CCGC 7 cut(s) 19, 25, 39, 78, 120, 152, 213
AcsI RAATTY 1 cut(s) 223
AfaI GTAC 2 cut(s) 230, 514
AgsI TTSAA 1 cut(s) 388
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 2 cut(s) 12, 89
AluI AGCT 2 cut(s) 12, 89
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 4 cut(s) 47, 186, 362, 489
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 2 cut(s) 148, 553
ApoI RAATTY 1 cut(s) 223
AseI ATTAAT 1 cut(s) 507
AspLEI GCGC 3 cut(s) 7, 148, 217
AspS9I GGNCC 1 cut(s) 257
AsuC2I CCSGG 1 cut(s) 260
AsuII TTCGAA 2 cut(s) 276, 489
AvaI CYCGRG 1 cut(s) 319
BanII GRGCYC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 135, 565
BccI CCATC 2 cut(s) 63, 629
BceAI ACGGC 2 cut(s) 187, 247
BciT130I CCWGG 1 cut(s) 163
BciVI GTATCC 1 cut(s) 256
BclI TGATCA 1 cut(s) 351
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 4 cut(s) 47, 186, 362, 489
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 3 cut(s) 149, 152, 554
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 3 cut(s) 150, 153, 555
Bme1390I CCNGG 2 cut(s) 163, 260
BmeT110I CYCGRG 1 cut(s) 319
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 170
BmrFI CCNGG 2 cut(s) 163, 260
BmrI ACTGGG 1 cut(s) 530
BmuI ACTGGG 1 cut(s) 530
BpmI CTGGAG 2 cut(s) 161, 184
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 1 cut(s) 8
Bpu14I TTCGAA 2 cut(s) 276, 489
BpuMI CCSGG 1 cut(s) 260
Bsa29I ATCGAT 1 cut(s) 607
BsaBI GATNNNNATC 1 cut(s) 607
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 320
Bse1I ACTGG 3 cut(s) 178, 302, 536
Bse8I GATNNNNATC 1 cut(s) 607
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 1 cut(s) 320
BseJI GATNNNNATC 1 cut(s) 607
BseMII CTCAG 3 cut(s) 99, 333, 459
BseNI ACTGG 3 cut(s) 178, 302, 536
BseRI GAGGAG 5 cut(s) 99, 161, 164, 359, 380
BseXI GCAGC 2 cut(s) 135, 565
Bsh1236I CGCG 2 cut(s) 215, 217
Bsh1285I CGRYCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 258
BshVI ATCGAT 1 cut(s) 607
BsiEI CGRYCG 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 2 cut(s) 260, 338
BsiWI CGTACG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 4 cut(s) 47, 186, 362, 489
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 258
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 319
Bsp119I TTCGAA 2 cut(s) 276, 489
Bsp1286I GDGCHC 1 cut(s) 14
Bsp143I GATC 3 cut(s) 303, 351, 608
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 7 cut(s) 19, 25, 39, 78, 120, 152, 213
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 3 cut(s) 98, 334, 460
BspDI ATCGAT 1 cut(s) 607
BspFNI CGCG 2 cut(s) 215, 217
BspLI GGNNCC 1 cut(s) 170
BspT104I TTCGAA 2 cut(s) 276, 489
BspTNI GGTCTC 1 cut(s) 47
BsrI ACTGG 3 cut(s) 178, 302, 536
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 3 cut(s) 303, 351, 608
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 159, 273
Bst6I CTCTTC 3 cut(s) 214, 396, 468
BstBI TTCGAA 2 cut(s) 276, 489
BstDEI CTNAG 4 cut(s) 8, 85, 342, 468
BstFNI CGCG 2 cut(s) 215, 217
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 148, 217
BstKTI GATC 3 cut(s) 306, 354, 611
BstMAI GTCTC 4 cut(s) 47, 186, 362, 489
BstMBI GATC 3 cut(s) 303, 351, 608
BstMCI CGRYCG 1 cut(s) 221
BstMWI GCNNNNNNNGC 3 cut(s) 18, 77, 86
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 161, 258
BstUI CGCG 2 cut(s) 215, 217
BstV1I GCAGC 2 cut(s) 135, 565
BstXI CCANNNNNNTGG 1 cut(s) 162
Bsu15I ATCGAT 1 cut(s) 607
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 258
BsuTUI ATCGAT 1 cut(s) 607
BtgZI GCGATG 1 cut(s) 41
BtrI CACGTC 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 295, 400
CfoI GCGC 3 cut(s) 7, 148, 217
Cfr13I GGNCC 1 cut(s) 257
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 2 cut(s) 229, 513
CviAII CATG 1 cut(s) 310
CviJI RGCY 9 cut(s) 12, 89, 169, 258, 301, 334, 341, 384, 450
CviKI_1 RGCY 9 cut(s) 12, 89, 169, 258, 301, 334, 341, 384, 450
CviQI GTAC 2 cut(s) 229, 513
DdeI CTNAG 4 cut(s) 8, 85, 342, 468
DpnI GATC 3 cut(s) 305, 353, 610
DpnII GATC 3 cut(s) 303, 351, 608
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 3 cut(s) 214, 396, 468
EarI CTCTTC 3 cut(s) 214, 396, 468
EciI GGCGGA 2 cut(s) 8, 14
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 605
Eco53kI GAGCTC 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 161
EcoRV GATATC 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 14
FaeI CATG 1 cut(s) 313
FaqI GGGAC 1 cut(s) 338
FatI CATG 1 cut(s) 309
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 3 cut(s) 149, 152, 554
FriOI GRGCYC 1 cut(s) 14
Fsp4HI GCNGC 3 cut(s) 149, 152, 554
GlaI GCGC 3 cut(s) 6, 147, 216
GluI GCNGC 3 cut(s) 149, 152, 554
GsuI CTGGAG 2 cut(s) 161, 184
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 258
HapII CCGG 2 cut(s) 260, 338
HhaI GCGC 3 cut(s) 7, 148, 217
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 3 cut(s) 5, 146, 215
HinP1I GCGC 3 cut(s) 5, 146, 215
HinfI GANTC 3 cut(s) 476, 567, 586
HpaII CCGG 2 cut(s) 260, 338
Hpy188I TCNGA 3 cut(s) 133, 186, 469
Hpy188III TCNNGA 2 cut(s) 355, 590
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 3 cut(s) 272, 485, 543
HpyCH4III ACNGT 2 cut(s) 159, 273
HpyCH4IV ACGT 2 cut(s) 128, 188
HpyCH4V TGCA 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 77, 86
HpyF3I CTNAG 4 cut(s) 8, 85, 342, 468
HpySE526I ACGT 2 cut(s) 128, 188
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 3 cut(s) 5, 146, 215
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 3 cut(s) 303, 351, 608
LmnI GCTCC 3 cut(s) 65, 94, 174
Lsp1109I GCAGC 2 cut(s) 135, 565
MaeII ACGT 2 cut(s) 128, 188
MaeIII GTNAC 3 cut(s) 284, 477, 590
MalI GATC 3 cut(s) 305, 353, 610
MboI GATC 3 cut(s) 303, 351, 608
MboII GAAGA 5 cut(s) 201, 413, 427, 485, 576
MhlI GDGCHC 1 cut(s) 14
MluCI AATT 2 cut(s) 223, 358
MlyI GAGTC 1 cut(s) 485
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 2 cut(s) 260, 338
MspR9I CCNGG 2 cut(s) 163, 260
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 2 cut(s) 215, 217
MwoI GCNNNNNNNGC 3 cut(s) 18, 77, 86
NciI CCSGG 1 cut(s) 260
NdeII GATC 3 cut(s) 303, 351, 608
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 170
NmuCI GTSAC 3 cut(s) 284, 477, 590
NspV TTCGAA 2 cut(s) 276, 489
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PfeI GAWTC 2 cut(s) 567, 586
Pfl23II CGTACG 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 3 cut(s) 150, 153, 555
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PshBI ATTAAT 1 cut(s) 507
Psp124BI GAGCTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 170
PspPI GGNCC 1 cut(s) 257
RsaI GTAC 2 cut(s) 230, 514
RsaNI GTAC 2 cut(s) 229, 513
RseI CAYNNNNRTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 3 cut(s) 149, 152, 554
Sau3AI GATC 3 cut(s) 303, 351, 608
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 163, 260
SduI GDGCHC 1 cut(s) 14
SetI ASST 7 cut(s) 14, 77, 91, 131, 191, 197, 459
SfuI TTCGAA 2 cut(s) 276, 489
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 2 cut(s) 223, 358
SsiI CCGC 7 cut(s) 19, 25, 39, 78, 120, 152, 213
SstI GAGCTC 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 161, 258
TaaI ACNGT 2 cut(s) 159, 273
TaiI ACGT 2 cut(s) 131, 191
TaqI TCGA 3 cut(s) 276, 489, 607
TaqII GACCGA 1 cut(s) 235
TasI AATT 2 cut(s) 223, 358
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 2 cut(s) 567, 586
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 2 cut(s) 302, 400
TseFI GTSAC 3 cut(s) 284, 477, 590
TseI GCWGC 2 cut(s) 148, 553
Tsp45I GTSAC 3 cut(s) 284, 477, 590
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 2 cut(s) 302, 400
VspI ATTAAT 1 cut(s) 507
XapI RAATTY 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.