Rh5AG533900

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005120
Physical Location & Seq
Reverse (-)
91597078 .. 91655995
58918 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG533900.1

Sequence Viewer

Length: 1392 bp
ATGGCGCAGAACCTTCTCCGCCTCCGGCGAAGCCTAAGCAGCAGCAGCCTCGTCGCAAATCTCCACCGTTCTCTATCGGCCACCTCCGCACTCTCCTCTGTCCCACGCTCCTCTTCCCCTTCCCCCTCCTCCTCCCCCGCCGTTGTTCAATCTCTCCAATTCCGATTCCAATCTCGATCATTCAGGTCGTCCTCCTTCTCTTCTGCACGGCCGCCTTACGGTAGCAACAACCAAAATGACGAGATTGGCCCCGACACCATACTCTTCGAAGGCTGCGATTACAACCACTGGCTCATCGTCATGGATTTCCCCAGAGATAACAAGCCTTCTCCCGAAGAGATGGTCAGAACTTATGAGGAAACCTGTGCCAGAGGCTTGAATATCAGTGTGGAAGAGGCAAAAAAGAAAATTTATGCTTGTAGCACAACAACGTATACAGGCTTTCAGGTGTTGATGACTGAGGAAGAGTCTGAGAAGTTTAATGGTGTACCTGGAGTTATTTTTGTGTTGCCTGATTCTTACATTGATCCACAAAACAAGGAGTATGGAGGAGACAAATATGATAATGGGAAAATTACACCCAGACCGCCCCCATTTCAGTATGAGAGGCAGGGCTCAAGATACCGTGATCGTAACAGATACGAGCGACAAGGACCAATGCCAAATCAGCAAGGGAATCCCTCGTATGGCCAACAGGGTTCTCCACAAGGTGGAGGAAACTATAGGACTTCACAAAATTATCCACACCAGACTTATGCCCCACCAGGACAAGGGGAACACAGAGGTCATACACCAGTTAATTCTCCTGGAGGAAGGGACGCATATCAGCCAGGTGGAGGTCCAACTCCTTCATATCAGGGCAATTACAACCAACGGGTGCAAGGGAACAGACCACAAGGAGAGCAGAGTACATATGCTCCTCCTGGTCAAGGGGAATATAGGGGTAATGATGGGACTTATGGTTATGGGCAAGGTCCAAGCTCTGGAACTCATGGACATGGTTTGAGTGGGCTTCAAGGCCCGGGAACAGGTGGATCTTATGGGCAAGGACTGAGTGGTGGATCTTATGGACAAGGACCGAGTGGTGGAGGTTATGGGCAAGGACCGAGTGCTGCAGGTTATGGGCAAGGACCGAGTGCTGCAGGTTATGGGCAAGGACCGAGTGCCACAGGTTATGGTCAAGGACCGAGTGCCGGAAGTTATGGCCAAGGACCGGGAGCTGGAGCTTGTGGACAAGGACCCGTATCTAGTGCTCAAGTCCAAGGACCCAGTGCTGGCTACCCTGGGCATGGAGATCAGAGTTGCACCCAAGAGGAGCCAAGGAGAATGCAAGGAGAACAAACAAACTATGCATACATGGGACAGACAGGAACTGACCAAGGAAGATATTAG

Protein Analysis

463

Amino Acids

49.09

Weight (kDa)

7.63

Isoelectric Point (pI)

58.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 94 - 187 3.4e-37 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1106, 1133
AccB7I CCANNNNNTGG 2 cut(s) 710, 983
AccI GTMKAC 1 cut(s) 434
AciI CCGC 5 cut(s) 19, 87, 138, 212, 587
AclWI GGATC 3 cut(s) 521, 1042, 1069
AcoI YGGCCR 4 cut(s) 78, 209, 688, 1204
AcsI RAATTY 1 cut(s) 408
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 2 cut(s) 489, 910
AgsI TTSAA 3 cut(s) 149, 379, 1016
AjnI CCWGG 6 cut(s) 490, 763, 805, 829, 922, 1282
AluBI AGCT 3 cut(s) 981, 1220, 1226
AluI AGCT 3 cut(s) 981, 1220, 1226
Alw21I GWGCWC 1 cut(s) 1255
Alw26I GTCTC 1 cut(s) 546
AlwI GGATC 3 cut(s) 521, 1042, 1069
AlwNI CAGNNNCTG 1 cut(s) 1373
Ama87I CYCGRG 1 cut(s) 1021
AoxI GGCC 6 cut(s) 78, 209, 247, 688, 1018, 1204
ApeKI GCWGC 6 cut(s) 39, 42, 45, 273, 1112, 1139
ApoI RAATTY 1 cut(s) 408
ArsI GACNNNNNNTTYG 2 cut(s) 327, 359
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 3 cut(s) 1022, 1023, 1215
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 1021
BalI TGGCCA 2 cut(s) 690, 1206
BanII GRGCYC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 1255
BbvI GCAGC 6 cut(s) 51, 54, 57, 260, 1099, 1126
BccI CCATC 2 cut(s) 334, 944
BceAI ACGGC 2 cut(s) 125, 224
BcgI CGANNNNNNTGC 4 cut(s) 31, 34, 65, 68
BciT130I CCWGG 6 cut(s) 492, 765, 807, 831, 924, 1284
BcnI CCSGG 3 cut(s) 1022, 1023, 1215
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 1 cut(s) 1248
BfmI CTRYAG 3 cut(s) 721, 1113, 1140
BfuAI ACCTGC 2 cut(s) 1106, 1133
BisI GCNGC 7 cut(s) 40, 43, 46, 212, 274, 1113, 1140
BlsI GCNGC 7 cut(s) 41, 44, 47, 213, 275, 1114, 1141
Bme1390I CCNGG 9 cut(s) 492, 765, 807, 831, 924, 1022, 1023, 1215, 1284
BmeT110I CYCGRG 1 cut(s) 1021
BmiI GGNNCC 4 cut(s) 250, 1240, 1267, 1317
BmrFI CCNGG 9 cut(s) 492, 765, 807, 831, 924, 1022, 1023, 1215, 1284
BmrI ACTGGG 1 cut(s) 1263
BmuI ACTGGG 1 cut(s) 1263
BpmI CTGGAG 3 cut(s) 513, 828, 1242
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 601, 1239
BpuMI CCSGG 3 cut(s) 1022, 1023, 1215
BsaBI GATNNNNATC 1 cut(s) 169
BsaJI CCNNGG 7 cut(s) 1021, 1207, 1261, 1282, 1283, 1319, 1378
BsaXI ACNNNNNCTCC 2 cut(s) 901, 931
Bse1I ACTGG 3 cut(s) 293, 794, 1269
Bse8I GATNNNNATC 1 cut(s) 169
BseBI CCWGG 6 cut(s) 492, 765, 807, 831, 924, 1284
BseDI CCNNGG 7 cut(s) 1021, 1207, 1261, 1282, 1283, 1319, 1378
BseJI GATNNNNATC 1 cut(s) 169
BseMII CTCAG 3 cut(s) 450, 462, 1043
BseNI ACTGG 3 cut(s) 293, 794, 1269
BseRI GAGGAG 7 cut(s) 85, 100, 118, 121, 564, 909, 1328
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 6 cut(s) 51, 54, 57, 260, 1099, 1126
BsgI GTGCAG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 6 cut(s) 80, 211, 249, 690, 1020, 1206
BsiEI CGRYCG 1 cut(s) 212
BsiHKAI GWGCWC 1 cut(s) 1255
BsiHKCI CYCGRG 1 cut(s) 1021
BsiSI CCGG 4 cut(s) 25, 1022, 1194, 1214
BslFI GGGAC 4 cut(s) 86, 830, 967, 1374
BsmAI GTCTC 1 cut(s) 546
BsmFI GGGAC 4 cut(s) 86, 830, 967, 1374
BsmI GAATGC 1 cut(s) 1332
BsnI GGCC 6 cut(s) 80, 211, 249, 690, 1020, 1206
BsoBI CYCGRG 1 cut(s) 1021
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 617, 1255
Bsp143I GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
BspACI CCGC 5 cut(s) 19, 87, 138, 212, 587
BspANI GGCC 6 cut(s) 80, 211, 249, 690, 1020, 1206
BspCNI CTCAG 3 cut(s) 451, 463, 1044
BspLI GGNNCC 4 cut(s) 250, 1240, 1267, 1317
BspMAI CTGCAG 2 cut(s) 1117, 1144
BspMI ACCTGC 2 cut(s) 1106, 1133
BspPI GGATC 3 cut(s) 521, 1042, 1069
BspT104I TTCGAA 1 cut(s) 267
BsrI ACTGG 3 cut(s) 293, 794, 1269
BssECI CCNNGG 7 cut(s) 1021, 1207, 1261, 1282, 1283, 1319, 1378
BssMI GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
BssNAI GTATAC 1 cut(s) 435
BssT1I CCWWGG 4 cut(s) 1207, 1261, 1319, 1378
Bst1107I GTATAC 1 cut(s) 435
Bst2UI CCWGG 6 cut(s) 492, 765, 807, 831, 924, 1284
Bst4CI ACNGT 3 cut(s) 68, 221, 626
Bst6I CTCTTC 6 cut(s) 118, 205, 269, 330, 387, 459
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 1276
BstDEI CTNAG 4 cut(s) 35, 459, 471, 1052
BstENI CCTNNNNNAGG 1 cut(s) 927
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 179, 529, 631, 1037, 1064, 1297
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 4 cut(s) 39, 45, 86, 667
BstNI CCWGG 6 cut(s) 492, 765, 807, 831, 924, 1284
BstSCI CCNGG 9 cut(s) 490, 763, 805, 829, 922, 1020, 1021, 1213, 1282
BstSFI CTRYAG 3 cut(s) 721, 1113, 1140
BstV1I GCAGC 6 cut(s) 51, 54, 57, 260, 1099, 1126
BstX2I RGATCY 2 cut(s) 1034, 1061
BstYI RGATCY 2 cut(s) 1034, 1061
BstZ17I GTATAC 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 209
BsuRI GGCC 6 cut(s) 80, 211, 249, 690, 1020, 1206
BtsIMutI CAGTG 3 cut(s) 286, 391, 1276
BveI ACCTGC 2 cut(s) 1106, 1133
Cac8I GCNNGC 1 cut(s) 1276
CaiI CAGNNNCTG 1 cut(s) 1373
CfoI GCGC 1 cut(s) 7
Cfr9I CCCGGG 1 cut(s) 1021
CseI GACGC 1 cut(s) 827
Csp6I GTAC 2 cut(s) 488, 909
CviAII CATG 5 cut(s) 301, 992, 998, 1289, 1357
CviQI GTAC 2 cut(s) 488, 909
DdeI CTNAG 4 cut(s) 35, 459, 471, 1052
DpnI GATC 6 cut(s) 178, 528, 630, 1036, 1063, 1296
DpnII GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
DraIII CACNNNGTG 1 cut(s) 710
EaeI YGGCCR 4 cut(s) 78, 209, 688, 1204
EagI CGGCCG 1 cut(s) 209
Eam1104I CTCTTC 6 cut(s) 118, 205, 269, 330, 387, 459
EarI CTCTTC 6 cut(s) 118, 205, 269, 330, 387, 459
EciI GGCGGA 1 cut(s) 8
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 4 cut(s) 1207, 1261, 1319, 1378
Eco24I GRGCYC 1 cut(s) 617
Eco52I CGGCCG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 1021
EcoNI CCTNNNNNAGG 1 cut(s) 927
EcoO109I RGGNCCY 2 cut(s) 1238, 1265
EcoRII CCWGG 6 cut(s) 490, 763, 805, 829, 922, 1282
EcoT14I CCWWGG 4 cut(s) 1207, 1261, 1319, 1378
EcoT22I ATGCAT 1 cut(s) 1354
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 4 cut(s) 1207, 1261, 1319, 1378
FaeI CATG 5 cut(s) 304, 995, 1001, 1292, 1360
FaqI GGGAC 4 cut(s) 86, 830, 967, 1374
FatI CATG 5 cut(s) 300, 991, 997, 1288, 1356
FauI CCCGC 1 cut(s) 145
FauNDI CATATG 1 cut(s) 913
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 7 cut(s) 40, 43, 46, 212, 274, 1113, 1140
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 7 cut(s) 40, 43, 46, 212, 274, 1113, 1140
FspBI CTAG 1 cut(s) 1248
GlaI GCGC 1 cut(s) 6
GluI GCNGC 7 cut(s) 40, 43, 46, 212, 274, 1113, 1140
GsuI CTGGAG 3 cut(s) 513, 828, 1242
HaeIII GGCC 6 cut(s) 80, 211, 249, 690, 1020, 1206
HapII CCGG 4 cut(s) 25, 1022, 1194, 1214
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 304, 995, 1001, 1292, 1360
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 165, 467, 515, 676
HpaII CCGG 4 cut(s) 25, 1022, 1194, 1214
Hpy166II GTNNAC 3 cut(s) 435, 488, 1232
Hpy188I TCNGA 4 cut(s) 164, 347, 472, 1299
Hpy188III TCNNGA 4 cut(s) 174, 332, 618, 984
Hpy8I GTNNAC 3 cut(s) 435, 488, 1232
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 7 cut(s) 23, 129, 205, 263, 336, 807, 858
HpyCH4III ACNGT 3 cut(s) 68, 221, 626
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 7 cut(s) 206, 880, 1115, 1142, 1305, 1330, 1352
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 45, 86, 667
HpyF3I CTNAG 4 cut(s) 35, 459, 471, 1052
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 5 cut(s) 304, 995, 1001, 1292, 1360
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
LmnI GCTCC 5 cut(s) 113, 922, 1217, 1223, 1315
Lsp1109I GCAGC 6 cut(s) 51, 54, 57, 260, 1099, 1126
MaeI CTAG 1 cut(s) 1248
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 632
MalI GATC 6 cut(s) 178, 528, 630, 1036, 1063, 1296
MboI GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
MboII GAAGA 6 cut(s) 105, 192, 256, 347, 404, 476
MflI RGATCY 2 cut(s) 1034, 1061
MhlI GDGCHC 2 cut(s) 617, 1255
MlsI TGGCCA 2 cut(s) 690, 1206
MluCI AATT 6 cut(s) 158, 408, 573, 736, 799, 862
MluNI TGGCCA 2 cut(s) 690, 1206
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 866
Mox20I TGGCCA 2 cut(s) 690, 1206
Mph1103I ATGCAT 1 cut(s) 1354
MscI TGGCCA 2 cut(s) 690, 1206
MseI TTAA 2 cut(s) 480, 798
MslI CAYNNNNRTG 2 cut(s) 299, 996
Msp20I TGGCCA 2 cut(s) 690, 1206
MspI CCGG 4 cut(s) 25, 1022, 1194, 1214
MspR9I CCNGG 9 cut(s) 492, 765, 807, 831, 924, 1022, 1023, 1215, 1284
Mva1269I GAATGC 1 cut(s) 1332
MvaI CCWGG 6 cut(s) 492, 765, 807, 831, 924, 1284
MwoI GCNNNNNNNGC 4 cut(s) 39, 45, 86, 667
NciI CCSGG 3 cut(s) 1022, 1023, 1215
NdeI CATATG 1 cut(s) 913
NdeII GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
NlaIII CATG 5 cut(s) 304, 995, 1001, 1292, 1360
NlaIV GGNNCC 4 cut(s) 250, 1240, 1267, 1317
NsiI ATGCAT 1 cut(s) 1354
NspV TTCGAA 1 cut(s) 267
PasI CCCWGGG 1 cut(s) 1283
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 1 cut(s) 1332
PfeI GAWTC 3 cut(s) 165, 515, 676
PflMI CCANNNNNTGG 2 cut(s) 710, 983
PfoI TCCNGGA 1 cut(s) 805
PkrI GCNGC 7 cut(s) 41, 44, 47, 213, 275, 1114, 1141
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PpuMI RGGWCCY 2 cut(s) 1238, 1265
Psp5II RGGWCCY 2 cut(s) 1238, 1265
Psp6I CCWGG 6 cut(s) 490, 763, 805, 829, 922, 1282
PspGI CCWGG 6 cut(s) 490, 763, 805, 829, 922, 1282
PspN4I GGNNCC 4 cut(s) 250, 1240, 1267, 1317
PspPPI RGGWCCY 2 cut(s) 1238, 1265
PstI CTGCAG 2 cut(s) 1117, 1144
PstNI CAGNNNCTG 1 cut(s) 1373
PsuI RGATCY 2 cut(s) 1034, 1061
RsaI GTAC 2 cut(s) 489, 910
RsaNI GTAC 2 cut(s) 488, 909
RseI CAYNNNNRTG 2 cut(s) 299, 996
SaqAI TTAA 2 cut(s) 480, 798
SatI GCNGC 7 cut(s) 40, 43, 46, 212, 274, 1113, 1140
Sau3AI GATC 6 cut(s) 176, 526, 628, 1034, 1061, 1294
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 9 cut(s) 492, 765, 807, 831, 924, 1022, 1023, 1215, 1284
SduI GDGCHC 2 cut(s) 617, 1255
SfcI CTRYAG 3 cut(s) 721, 1113, 1140
SfuI TTCGAA 1 cut(s) 267
SmaI CCCGGG 1 cut(s) 1023
SmiMI CAYNNNNRTG 2 cut(s) 299, 996
SmlI CTYRAG 2 cut(s) 616, 1254
SmoI CTYRAG 2 cut(s) 616, 1254
Sse9I AATT 6 cut(s) 158, 408, 573, 736, 799, 862
SsiI CCGC 5 cut(s) 19, 87, 138, 212, 587
SspMI CTAG 1 cut(s) 1248
StyD4I CCNGG 9 cut(s) 490, 763, 805, 829, 922, 1020, 1021, 1213, 1282
StyI CCWWGG 4 cut(s) 1207, 1261, 1319, 1378
TaaI ACNGT 3 cut(s) 68, 221, 626
TaiI ACGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 175, 267
TaqII GACCGA 5 cut(s) 1093, 1120, 1147, 1174, 1201
TasI AATT 6 cut(s) 158, 408, 573, 736, 799, 862
TatI WGTACW 1 cut(s) 908
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 3 cut(s) 165, 515, 676
Tru1I TTAA 2 cut(s) 480, 798
Tru9I TTAA 2 cut(s) 480, 798
TscAI CASTG 3 cut(s) 293, 391, 1276
TseI GCWGC 6 cut(s) 39, 42, 45, 273, 1112, 1139
TspDTI ATGAA 1 cut(s) 840
TspMI CCCGGG 1 cut(s) 1021
TspRI CASTG 3 cut(s) 293, 391, 1276
Van91I CCANNNNNTGG 2 cut(s) 710, 983
XagI CCTNNNNNAGG 1 cut(s) 927
XapI RAATTY 1 cut(s) 408
XmaI CCCGGG 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 434
XspI CTAG 1 cut(s) 1248
Zsp2I ATGCAT 1 cut(s) 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.