RchiOBHm_Chr5g0083551

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005120
Physical Location & Seq
Reverse (-)
89258443 .. 89261021
2579 bp
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UTR
Exon/CDS
Intron
PRQ35769

Sequence Viewer

Length: 552 bp
ATGGCGCAGAACCTTCTCCGCCTCCGGCGAAGCCTAAGCAGCAGCCTCGTCGCAAATCTCCACCGTTCTCTATCGTCAACCTCCGCACTCTCCTCTGTCCCACGCTCCTCTTCCCCTTCCCCTTCCTCCTCCTCCTCCCCCGCCGTTGTTCAATCTCTCCAATTCCGATTCCAATCTCGATCATTCAGGTCGTCCTCCTTCTCTTCTGCACGGCCGCCTTACGGCAGCAACAACCAAAATGACGAGATTGGCCCCGACACCATACTCTTCGAAGGCTGCGATTACAACCACTGGCTCATCGTCATGGATTTCCCCAGAGATAACAAGCCTTCTCCCGAAGAGATGGTCAGAACTTATGAGGAAACCTGTGCTAGAGGCTTGAACATCAGTGTGGAAGAGGCAAAAAAGAAAATTTATGCTTGTAGCACAACAACGTATACAGGCTTTCAGGTGTTGATGACTGAGGAAGAGTCTGAGAAGTTTAATGGTGTACCTGGAGTTATTTTTGTGTTCCCGGATTCTTTATTTTTCTGCACGATACTGGAAAAATGA

Protein Analysis

183

Amino Acids

20.4

Weight (kDa)

6.14

Isoelectric Point (pI)

88.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 95 - 176 1.7e-31 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 437
AciI CCGC 4 cut(s) 19, 84, 141, 215
AcoI YGGCCR 1 cut(s) 212
AcsI RAATTY 1 cut(s) 411
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 2 cut(s) 152, 382
AjnI CCWGG 1 cut(s) 493
AoxI GGCC 2 cut(s) 212, 250
ApeKI GCWGC 4 cut(s) 39, 42, 225, 276
ApoI RAATTY 1 cut(s) 411
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 251
AsuC2I CCSGG 1 cut(s) 515
AsuII TTCGAA 1 cut(s) 270
BbvI GCAGC 4 cut(s) 51, 54, 237, 263
BccI CCATC 1 cut(s) 337
BceAI ACGGC 3 cut(s) 128, 227, 238
BcgI CGANNNNNNTGC 4 cut(s) 28, 31, 62, 65
BciT130I CCWGG 1 cut(s) 495
BcnI CCSGG 1 cut(s) 515
BfaI CTAG 1 cut(s) 372
BisI GCNGC 5 cut(s) 40, 43, 215, 226, 277
BlsI GCNGC 5 cut(s) 41, 44, 216, 227, 278
Bme1390I CCNGG 2 cut(s) 495, 515
BmgT120I GGNCC 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 2 cut(s) 495, 515
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 172
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 296, 546
Bse8I GATNNNNATC 1 cut(s) 172
BseBI CCWGG 1 cut(s) 495
BseJI GATNNNNATC 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 453, 465
BseNI ACTGG 2 cut(s) 296, 546
BseRI GAGGAG 5 cut(s) 82, 97, 118, 121, 124
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 4 cut(s) 51, 54, 237, 263
BsgI GTGCAG 2 cut(s) 192, 517
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 214, 252
BsiEI CGRYCG 1 cut(s) 215
BsiSI CCGG 2 cut(s) 25, 515
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 214, 252
Bsp119I TTCGAA 1 cut(s) 270
Bsp143I GATC 1 cut(s) 179
BspACI CCGC 4 cut(s) 19, 84, 141, 215
BspANI GGCC 2 cut(s) 214, 252
BspCNI CTCAG 2 cut(s) 454, 466
BspLI GGNNCC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 270
BsrI ACTGG 2 cut(s) 296, 546
BssMI GATC 1 cut(s) 179
BssNAI GTATAC 1 cut(s) 438
Bst1107I GTATAC 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 65
Bst6I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
BstBI TTCGAA 1 cut(s) 270
BstDEI CTNAG 3 cut(s) 35, 462, 474
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 182
BstMBI GATC 1 cut(s) 179
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstNI CCWGG 1 cut(s) 495
BstSCI CCNGG 2 cut(s) 493, 513
BstV1I GCAGC 4 cut(s) 51, 54, 237, 263
BstZ17I GTATAC 1 cut(s) 438
BstZI CGGCCG 1 cut(s) 212
BsuRI GGCC 2 cut(s) 214, 252
BtsIMutI CAGTG 2 cut(s) 289, 394
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 251
Csp6I GTAC 1 cut(s) 491
CviAII CATG 1 cut(s) 304
CviJI RGCY 9 cut(s) 33, 45, 214, 252, 276, 295, 328, 378, 444
CviKI_1 RGCY 9 cut(s) 33, 45, 214, 252, 276, 295, 328, 378, 444
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 3 cut(s) 35, 462, 474
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 212
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EarI CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EciI GGCGGA 1 cut(s) 8
EclXI CGGCCG 1 cut(s) 212
Eco52I CGGCCG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 493
FaeI CATG 1 cut(s) 307
FaiI YATR 5 cut(s) 263, 305, 357, 417, 438
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 148
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 5 cut(s) 40, 43, 215, 226, 277
Fsp4HI GCNGC 5 cut(s) 40, 43, 215, 226, 277
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 6
GluI GCNGC 5 cut(s) 40, 43, 215, 226, 277
GsuI CTGGAG 1 cut(s) 516
HaeIII GGCC 2 cut(s) 214, 252
HapII CCGG 2 cut(s) 25, 515
HhaI GCGC 1 cut(s) 7
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 3 cut(s) 168, 470, 518
HpaII CCGG 2 cut(s) 25, 515
Hpy166II GTNNAC 3 cut(s) 78, 438, 491
Hpy188I TCNGA 3 cut(s) 167, 350, 475
Hpy188III TCNNGA 2 cut(s) 177, 335
Hpy8I GTNNAC 3 cut(s) 78, 438, 491
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 6 cut(s) 23, 126, 132, 208, 266, 339
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 2 cut(s) 209, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 3 cut(s) 35, 462, 474
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 4 cut(s) 51, 54, 237, 263
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 434
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MboII GAAGA 6 cut(s) 102, 195, 259, 350, 407, 479
MluCI AATT 2 cut(s) 161, 411
MlyI GAGTC 1 cut(s) 479
MseI TTAA 1 cut(s) 483
MslI CAYNNNNRTG 2 cut(s) 302, 389
MspI CCGG 2 cut(s) 25, 515
MspR9I CCNGG 2 cut(s) 495, 515
MvaI CCWGG 1 cut(s) 495
MwoI GCNNNNNNNGC 1 cut(s) 39
NciI CCSGG 1 cut(s) 515
NdeII GATC 1 cut(s) 179
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PfeI GAWTC 2 cut(s) 168, 518
PfoI TCCNGGA 1 cut(s) 513
PkrI GCNGC 5 cut(s) 41, 44, 216, 227, 278
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 1 cut(s) 251
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 2 cut(s) 302, 389
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 5 cut(s) 40, 43, 215, 226, 277
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 1 cut(s) 251
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 495, 515
SetI ASST 7 cut(s) 15, 83, 191, 368, 437, 453, 496
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 302, 389
Sse9I AATT 2 cut(s) 161, 411
SsiI CCGC 4 cut(s) 19, 84, 141, 215
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 2 cut(s) 493, 513
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 437
TaqI TCGA 2 cut(s) 178, 270
TasI AATT 2 cut(s) 161, 411
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 2 cut(s) 168, 518
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 2 cut(s) 296, 394
TseI GCWGC 4 cut(s) 39, 42, 225, 276
TspRI CASTG 2 cut(s) 296, 394
XapI RAATTY 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.