Rorug05G0484500

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005120
Physical Location & Seq
Forward (+)
66772227 .. 66772700
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0484500.1

Sequence Viewer

Length: 474 bp
ATGTCGCTCATCCCCAGCTTCCGAAGAAGCAGCGTCTTTGATCTCTTTTCCCTCGATCTCTGGGACCCTTTCAAGGATTTCCAATTCCCTGCTTCATCGCTCTCCACTTTCCCTGAATTTCCTCTCGAAAATTCGGCTTTTGTTAACACCAGGATCGACTGGAAGGAGACTCCAGAAGCCCATGTGTTCAAGGCCGACATTCCGGGGCTGAAGAAAGAAGAGGTCAAGGTTGAGGTTGAAGATGACAGGGTGCTTCAGATTAGCGGAGAGAGGAAGATAGAGAAGGAGGACAAGAACGACACCTGGCACCGGGTCGAGCGTAGCAGCGGCAAGTTCTCCAGAAGGTTCAGGCTTCCTGAGAATGCCAAGATAGACGAGATCAAGGCTGCTATGGAGAATGGGGTTCTCAGCGTGACAATTCCTAAGGTGGAGGTGAATAAGCCCGATGTCAAAGCTATTGAAATCTCCGGTTGA

Protein Analysis

157

Amino Acids

18.07

Weight (kDa)

5.36

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 53 - 156 1.5e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 306
AciI CCGC 2 cut(s) 264, 327
AclWI GGATC 1 cut(s) 161
AcsI RAATTY 2 cut(s) 116, 130
AcuI CTGAAG 2 cut(s) 230, 239
AfiI CCNNNNNNNGG 2 cut(s) 73, 309
AgsI TTSAA 4 cut(s) 73, 190, 239, 461
AjnI CCWGG 2 cut(s) 149, 302
AluBI AGCT 2 cut(s) 18, 455
AluI AGCT 2 cut(s) 18, 455
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 161
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 3 cut(s) 30, 324, 386
ApoI RAATTY 2 cut(s) 116, 130
AspS9I GGNCC 1 cut(s) 64
AsuC2I CCSGG 2 cut(s) 204, 311
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 423
BanI GGYRCC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 42, 336, 373
BciT130I CCWGG 2 cut(s) 151, 304
BcnI CCSGG 2 cut(s) 204, 311
BcoDI GTCTC 1 cut(s) 161
BisI GCNGC 4 cut(s) 31, 325, 328, 387
BlsI GCNGC 4 cut(s) 32, 326, 329, 388
Bme1390I CCNGG 4 cut(s) 151, 204, 304, 311
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmiI GGNNCC 3 cut(s) 65, 66, 308
BmrFI CCNGG 4 cut(s) 151, 204, 304, 311
BpmI CTGGAG 2 cut(s) 156, 322
BpuMI CCSGG 2 cut(s) 204, 311
BsaJI CCNNGG 1 cut(s) 203
BsaWI WCCGGW 1 cut(s) 467
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 309
Bse1I ACTGG 1 cut(s) 164
Bse21I CCTNAGG 1 cut(s) 423
BseBI CCWGG 2 cut(s) 151, 304
BseDI CCNNGG 1 cut(s) 203
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 73, 309
BseMII CTCAG 2 cut(s) 348, 421
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 3 cut(s) 42, 336, 373
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 1 cut(s) 194
BshNI GGYRCC 1 cut(s) 306
BsiSI CCGG 3 cut(s) 203, 310, 468
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 73, 309
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 1 cut(s) 194
Bsp143I GATC 4 cut(s) 40, 55, 153, 378
BspACI CCGC 2 cut(s) 264, 327
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 2 cut(s) 349, 420
BspLI GGNNCC 3 cut(s) 65, 66, 308
BspPI GGATC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 306
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 203
BssMI GATC 4 cut(s) 40, 55, 153, 378
Bst2UI CCWGG 2 cut(s) 151, 304
Bst6I CTCTTC 1 cut(s) 213
BstDEI CTNAG 3 cut(s) 357, 407, 423
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 4 cut(s) 43, 58, 156, 381
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 4 cut(s) 40, 55, 153, 378
BstNI CCWGG 2 cut(s) 151, 304
BstSCI CCNGG 4 cut(s) 149, 202, 302, 309
BstV1I GCAGC 3 cut(s) 42, 336, 373
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 64
CseI GACGC 1 cut(s) 22
CviAII CATG 1 cut(s) 182
CviJI RGCY 9 cut(s) 18, 137, 179, 194, 208, 352, 386, 442, 455
CviKI_1 RGCY 9 cut(s) 18, 137, 179, 194, 208, 352, 386, 442, 455
DdeI CTNAG 3 cut(s) 357, 407, 423
DpnI GATC 4 cut(s) 42, 57, 155, 380
DpnII GATC 4 cut(s) 40, 55, 153, 378
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 230, 239
Eco81I CCTNAGG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 2 cut(s) 149, 302
FaeI CATG 1 cut(s) 185
FaiI YATR 2 cut(s) 183, 392
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 181
Fnu4HI GCNGC 4 cut(s) 31, 325, 328, 387
Fsp4HI GCNGC 4 cut(s) 31, 325, 328, 387
GluI GCNGC 4 cut(s) 31, 325, 328, 387
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 2 cut(s) 156, 322
HaeIII GGCC 1 cut(s) 194
HapII CCGG 3 cut(s) 203, 310, 468
HgaI GACGC 1 cut(s) 22
Hin1II CATG 1 cut(s) 185
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 1 cut(s) 169
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 3 cut(s) 203, 310, 468
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 23, 258
Hpy188III TCNNGA 4 cut(s) 125, 173, 339, 356
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 3 cut(s) 157, 277, 336
HpyF3I CTNAG 3 cut(s) 357, 407, 423
Hsp92II CATG 1 cut(s) 185
KflI GGGWCCC 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 4 cut(s) 40, 55, 153, 378
Lsp1109I GCAGC 3 cut(s) 42, 336, 373
MaeIII GTNAC 1 cut(s) 412
MalI GATC 4 cut(s) 42, 57, 155, 380
MboI GATC 4 cut(s) 40, 55, 153, 378
MboII GAAGA 5 cut(s) 36, 223, 230, 251, 286
MluCI AATT 4 cut(s) 83, 116, 130, 417
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 7 cut(s) 62, 132, 214, 226, 264, 280, 424
MseI TTAA 1 cut(s) 144
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 3 cut(s) 203, 310, 468
MspR9I CCNGG 4 cut(s) 151, 204, 304, 311
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 2 cut(s) 151, 304
NciI CCSGG 2 cut(s) 204, 311
NdeII GATC 4 cut(s) 40, 55, 153, 378
NlaIII CATG 1 cut(s) 185
NlaIV GGNNCC 3 cut(s) 65, 66, 308
NmuCI GTSAC 1 cut(s) 412
PctI GAATGC 1 cut(s) 367
PkrI GCNGC 4 cut(s) 32, 326, 329, 388
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 149, 302
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 2 cut(s) 149, 302
PspN4I GGNNCC 3 cut(s) 65, 66, 308
PspPI GGNCC 1 cut(s) 64
PspPPI RGGWCCY 1 cut(s) 64
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 4 cut(s) 31, 325, 328, 387
Sau3AI GATC 4 cut(s) 40, 55, 153, 378
Sau96I GGNCC 1 cut(s) 64
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 4 cut(s) 151, 204, 304, 311
SetI ASST 9 cut(s) 20, 225, 231, 237, 305, 347, 429, 435, 457
SinI GGWCC 1 cut(s) 64
Sse9I AATT 4 cut(s) 83, 116, 130, 417
SsiI CCGC 2 cut(s) 264, 327
StyD4I CCNGG 4 cut(s) 149, 202, 302, 309
TaqI TCGA 4 cut(s) 54, 126, 156, 315
TasI AATT 4 cut(s) 83, 116, 130, 417
TauI GCSGC 1 cut(s) 330
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 3 cut(s) 30, 324, 386
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 2 cut(s) 116, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.