Rw5G049890

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005120
Physical Location & Seq
Forward (+)
86482784 .. 86486238
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049890.1

Sequence Viewer

Length: 1425 bp
ATGGCGCAGAACCTTCTCCGCCTCCGGCGAAGCCTAAGCAGCAGCCTCGTCGCAAATCTCCACCGCTCTCTATCGTCCACCTCCGCACTCTCCTCTGTCCCACGCTCCTCTTCCCCTTCCCCTTCCTCCTCCTCCTCCTCCGCCGTTGTCCAATCTCTCCAATTCCGATTCCAATCTCGATCATTCAGGTCGTCCTCCTTCTCTTCTGCACGGCCGCCTTACGGTAGCAACAACCAAAATGACGAGATTGGCCCCGACACCATACTCTTCGAAGGCTGCGATTACAACCACTGGCTCATCGTCATGGATTTCCCCAGAGATAACAAGCCTTCTCCCGAAGAGATGGTCAGAACTTATGAGGAAACCTGTGCCAGAGGCTTGAATATCAGTGTGGAAGAGGCAAAAAAGAAAATTTATGCTTGTAGCACAACAACGTATACAGGCTTTCAGGTGTTGATGACTGAGGAAGAGTCTGAGAAGTTTAATGGTGTACCTGGAGTTATTTTTGTGTTGCCCGATTCTTACATTGATCCACAAAACAAGGAGTATGGAGGAGACAAATATGATAATGGGAAAATTACACCCAGACCGCCCCCATTTCAGTATGAGAGGCAGGGCTCAAGATACCGTGATCGTAACAGATACGAGCGACAAGGAGCAATGCCAAATCAGCAAGGGAATCCCTCATATGGCCAACAGGGTTCTCCACAAGGTGGAGGAAACTATAGGACTTCACAAAATTATCCACACCAGACTTATGCCCCACCAGGACAAGGGGAACACAGAGGTCATACACCAGTTAATTCTCCTGGAGGAAGGGACGCATATCAGCCAGGTAGAGGTCCAACTCCTTCATATCAGGGCAATTACAACCAACAGGTGCAAGGGAACAGACCACAAGGAGAGCAGAGTACATATGCTCCTCCTGATCAAGGGGAATATAGGGGTAATGATGGGACTTATGGTTATGGGCAAGCTCCAAGCTCTGGAACTCATGGACATGGTTTGAGTGGGCTTCAAGGCCCGGGAACAGGTGGATCTTATGGGCAAGGACCGAGTGGTGGATCTTATGGACAAGGACCGAGTGGTGGAGGTTATGGGCAAGGACCGAGTGCCCCAGGTTATGGGCAAGGACCGAGTGCCCCAGGTTATGGTCAAGGACCGAGTGCTGGAAGTTATGGCCAAGGATCGAGATCTGGAGCTTCTGGACAAGGACCTGTATCTAGTGCTCAAGTCCAAGGACCCAGTGCTGGCTACCCTGGGCATGGAGATCAGAGTTTCACCCAAGAGGAGCCAAGGAGAATGCAAGGAGAACAAACAAATTATGCATACATGGGACAGACAGGAACTGACCAATCTCTTATTAAAAATTATAAAAATTTGAACTTGCAATGCCCCAATGGTAGCAATTACTATAATGTTTAA

Protein Analysis

474

Amino Acids

50.82

Weight (kDa)

8.15

Isoelectric Point (pI)

59.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 95 - 188 3.6e-37 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1374
AccB7I CCANNNNNTGG 4 cut(s) 713, 986, 1124, 1151
AccBSI CCGCTC 1 cut(s) 66
AccI GTMKAC 1 cut(s) 437
AciI CCGC 6 cut(s) 19, 64, 84, 141, 215, 590
AclWI GGATC 4 cut(s) 524, 1045, 1072, 1195
AcoI YGGCCR 3 cut(s) 212, 691, 1180
AcsI RAATTY 2 cut(s) 411, 1378
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 2 cut(s) 492, 913
AgsI TTSAA 3 cut(s) 382, 1019, 1384
AjnI CCWGG 7 cut(s) 493, 766, 808, 832, 1117, 1144, 1258
AluBI AGCT 3 cut(s) 977, 984, 1202
AluI AGCT 3 cut(s) 977, 984, 1202
Alw21I GWGCWC 1 cut(s) 1231
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 4 cut(s) 524, 1045, 1072, 1195
AlwNI CAGNNNCTG 1 cut(s) 1349
Ama87I CYCGRG 1 cut(s) 1024
AoxI GGCC 5 cut(s) 212, 250, 691, 1021, 1180
ApeKI GCWGC 3 cut(s) 39, 42, 276
ApoI RAATTY 2 cut(s) 411, 1378
ArsI GACNNNNNNTTYG 2 cut(s) 330, 362
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 2 cut(s) 1025, 1026
AsuHPI GGTGA 1 cut(s) 1273
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 1024
AvaII GGWCC 8 cut(s) 842, 1052, 1079, 1106, 1133, 1160, 1214, 1241
BaeGI GKGCMC 2 cut(s) 1117, 1144
BalI TGGCCA 2 cut(s) 693, 1182
BanII GRGCYC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 1231
BbvI GCAGC 3 cut(s) 51, 54, 263
BccI CCATC 2 cut(s) 337, 947
BceAI ACGGC 2 cut(s) 128, 227
BcgI CGANNNNNNTGC 4 cut(s) 28, 31, 62, 65
BciT130I CCWGG 7 cut(s) 495, 768, 810, 834, 1119, 1146, 1260
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 2 cut(s) 1025, 1026
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 1 cut(s) 1224
BfmI CTRYAG 1 cut(s) 724
BglII AGATCT 1 cut(s) 1193
BisI GCNGC 4 cut(s) 40, 43, 215, 277
BlsI GCNGC 4 cut(s) 41, 44, 216, 278
Bme1390I CCNGG 9 cut(s) 495, 768, 810, 834, 1025, 1026, 1119, 1146, 1260
Bme18I GGWCC 8 cut(s) 842, 1052, 1079, 1106, 1133, 1160, 1214, 1241
BmeT110I CYCGRG 1 cut(s) 1024
BmiI GGNNCC 3 cut(s) 253, 1243, 1293
BmrFI CCNGG 9 cut(s) 495, 768, 810, 834, 1025, 1026, 1119, 1146, 1260
BmrI ACTGGG 1 cut(s) 1239
BmuI ACTGGG 1 cut(s) 1239
BpmI CTGGAG 3 cut(s) 516, 831, 1218
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 2 cut(s) 604, 1215
BpuMI CCSGG 2 cut(s) 1025, 1026
BsaBI GATNNNNATC 2 cut(s) 172, 1192
BsaJI CCNNGG 8 cut(s) 1024, 1117, 1144, 1183, 1237, 1258, 1259, 1295
BsaXI ACNNNNNCTCC 2 cut(s) 904, 934
Bse1I ACTGG 3 cut(s) 296, 797, 1245
Bse3DI GCAATG 2 cut(s) 666, 1397
Bse8I GATNNNNATC 2 cut(s) 172, 1192
BseBI CCWGG 7 cut(s) 495, 768, 810, 834, 1119, 1146, 1260
BseDI CCNNGG 8 cut(s) 1024, 1117, 1144, 1183, 1237, 1258, 1259, 1295
BseJI GATNNNNATC 2 cut(s) 172, 1192
BseMI GCAATG 2 cut(s) 666, 1397
BseMII CTCAG 2 cut(s) 453, 465
BseNI ACTGG 3 cut(s) 296, 797, 1245
BseRI GAGGAG 9 cut(s) 82, 97, 118, 121, 124, 127, 567, 912, 1304
BseSI GKGCMC 2 cut(s) 1117, 1144
BseX3I CGGCCG 1 cut(s) 212
BseXI GCAGC 3 cut(s) 51, 54, 263
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 215
BshFI GGCC 5 cut(s) 214, 252, 693, 1023, 1182
BsiEI CGRYCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 1231
BsiHKCI CYCGRG 1 cut(s) 1024
BsiSI CCGG 2 cut(s) 25, 1025
BslFI GGGAC 4 cut(s) 83, 833, 970, 1350
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 4 cut(s) 83, 833, 970, 1350
BsmI GAATGC 1 cut(s) 1308
BsnI GGCC 5 cut(s) 214, 252, 693, 1023, 1182
BsoBI CYCGRG 1 cut(s) 1024
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 4 cut(s) 620, 1117, 1144, 1231
Bsp143I GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
BspACI CCGC 6 cut(s) 19, 64, 84, 141, 215, 590
BspANI GGCC 5 cut(s) 214, 252, 693, 1023, 1182
BspCNI CTCAG 2 cut(s) 454, 466
BspLI GGNNCC 3 cut(s) 253, 1243, 1293
BspPI GGATC 4 cut(s) 524, 1045, 1072, 1195
BspT104I TTCGAA 1 cut(s) 270
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 666, 1397
BsrI ACTGG 3 cut(s) 296, 797, 1245
BssECI CCNNGG 8 cut(s) 1024, 1117, 1144, 1183, 1237, 1258, 1259, 1295
BssMI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
BssNAI GTATAC 1 cut(s) 438
BssT1I CCWWGG 3 cut(s) 1183, 1237, 1295
Bst1107I GTATAC 1 cut(s) 438
Bst2UI CCWGG 7 cut(s) 495, 768, 810, 834, 1119, 1146, 1260
Bst4CI ACNGT 2 cut(s) 224, 629
Bst6I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 975, 1252
BstDEI CTNAG 3 cut(s) 35, 462, 474
BstENI CCTNNNNNAGG 1 cut(s) 930
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 9 cut(s) 182, 532, 634, 931, 1040, 1067, 1190, 1196, 1273
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
BstMCI CGRYCG 1 cut(s) 215
BstMWI GCNNNNNNNGC 2 cut(s) 39, 670
BstNI CCWGG 7 cut(s) 495, 768, 810, 834, 1119, 1146, 1260
BstSCI CCNGG 9 cut(s) 493, 766, 808, 832, 1023, 1024, 1117, 1144, 1258
BstSFI CTRYAG 1 cut(s) 724
BstSLI GKGCMC 2 cut(s) 1117, 1144
BstV1I GCAGC 3 cut(s) 51, 54, 263
BstX2I RGATCY 3 cut(s) 1037, 1064, 1193
BstYI RGATCY 3 cut(s) 1037, 1064, 1193
BstZ17I GTATAC 1 cut(s) 438
BstZI CGGCCG 1 cut(s) 212
BsuRI GGCC 5 cut(s) 214, 252, 693, 1023, 1182
BtsIMutI CAGTG 3 cut(s) 289, 394, 1252
Cac8I GCNNGC 2 cut(s) 975, 1252
CaiI CAGNNNCTG 1 cut(s) 1349
CfoI GCGC 1 cut(s) 7
Cfr9I CCCGGG 1 cut(s) 1024
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 491, 912
CviAII CATG 5 cut(s) 304, 995, 1001, 1265, 1333
CviQI GTAC 2 cut(s) 491, 912
DdeI CTNAG 3 cut(s) 35, 462, 474
DpnI GATC 9 cut(s) 181, 531, 633, 930, 1039, 1066, 1189, 1195, 1272
DpnII GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 3 cut(s) 212, 691, 1180
EagI CGGCCG 1 cut(s) 212
Eam1104I CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EarI CTCTTC 6 cut(s) 115, 208, 272, 333, 390, 462
EciI GGCGGA 2 cut(s) 8, 130
EclXI CGGCCG 1 cut(s) 212
Eco130I CCWWGG 3 cut(s) 1183, 1237, 1295
Eco24I GRGCYC 1 cut(s) 620
Eco47I GGWCC 8 cut(s) 842, 1052, 1079, 1106, 1133, 1160, 1214, 1241
Eco52I CGGCCG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 1024
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoO109I RGGNCCY 2 cut(s) 1214, 1241
EcoRII CCWGG 7 cut(s) 493, 766, 808, 832, 1117, 1144, 1258
EcoT14I CCWWGG 3 cut(s) 1183, 1237, 1295
EcoT22I ATGCAT 1 cut(s) 1330
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 3 cut(s) 1183, 1237, 1295
FaeI CATG 5 cut(s) 307, 998, 1004, 1268, 1336
FaqI GGGAC 4 cut(s) 83, 833, 970, 1350
FatI CATG 5 cut(s) 303, 994, 1000, 1264, 1332
FauNDI CATATG 2 cut(s) 688, 916
FbaI TGATCA 1 cut(s) 928
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 4 cut(s) 40, 43, 215, 277
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 4 cut(s) 40, 43, 215, 277
FspBI CTAG 1 cut(s) 1224
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 40, 43, 215, 277
GsuI CTGGAG 3 cut(s) 516, 831, 1218
HaeIII GGCC 5 cut(s) 214, 252, 693, 1023, 1182
HapII CCGG 2 cut(s) 25, 1025
HgaI GACGC 1 cut(s) 830
HhaI GCGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 307, 998, 1004, 1268, 1336
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 168, 470, 518, 679
HpaII CCGG 2 cut(s) 25, 1025
HphI GGTGA 1 cut(s) 1273
Hpy166II GTNNAC 3 cut(s) 78, 438, 491
Hpy188I TCNGA 4 cut(s) 167, 350, 475, 1275
Hpy188III TCNNGA 8 cut(s) 177, 335, 621, 926, 987, 1191, 1197, 1206
Hpy8I GTNNAC 3 cut(s) 78, 438, 491
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 8 cut(s) 23, 126, 132, 208, 266, 339, 810, 861
HpyCH4III ACNGT 2 cut(s) 224, 629
HpyCH4IV ACGT 1 cut(s) 434
HpyCH4V TGCA 5 cut(s) 209, 883, 1306, 1328, 1390
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 670
HpyF3I CTNAG 3 cut(s) 35, 462, 474
HpySE526I ACGT 1 cut(s) 434
Hsp92II CATG 5 cut(s) 307, 998, 1004, 1268, 1336
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
LmnI GCTCC 6 cut(s) 110, 656, 925, 982, 1199, 1291
Lsp1109I GCAGC 3 cut(s) 51, 54, 263
MaeI CTAG 1 cut(s) 1224
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 1 cut(s) 635
MalI GATC 9 cut(s) 181, 531, 633, 930, 1039, 1066, 1189, 1195, 1272
MbiI CCGCTC 1 cut(s) 66
MboI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
MboII GAAGA 6 cut(s) 102, 195, 259, 350, 407, 479
MflI RGATCY 3 cut(s) 1037, 1064, 1193
MhlI GDGCHC 4 cut(s) 620, 1117, 1144, 1231
MlsI TGGCCA 2 cut(s) 693, 1182
MluNI TGGCCA 2 cut(s) 693, 1182
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 869
Mox20I TGGCCA 2 cut(s) 693, 1182
Mph1103I ATGCAT 1 cut(s) 1330
MscI TGGCCA 2 cut(s) 693, 1182
MseI TTAA 4 cut(s) 483, 801, 1365, 1423
MslI CAYNNNNRTG 2 cut(s) 302, 999
Msp20I TGGCCA 2 cut(s) 693, 1182
MspI CCGG 2 cut(s) 25, 1025
MspR9I CCNGG 9 cut(s) 495, 768, 810, 834, 1025, 1026, 1119, 1146, 1260
Mva1269I GAATGC 1 cut(s) 1308
MvaI CCWGG 7 cut(s) 495, 768, 810, 834, 1119, 1146, 1260
MwoI GCNNNNNNNGC 2 cut(s) 39, 670
NciI CCSGG 2 cut(s) 1025, 1026
NdeI CATATG 2 cut(s) 688, 916
NdeII GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
NlaIII CATG 5 cut(s) 307, 998, 1004, 1268, 1336
NlaIV GGNNCC 3 cut(s) 253, 1243, 1293
NsiI ATGCAT 1 cut(s) 1330
NspV TTCGAA 1 cut(s) 270
PasI CCCWGGG 1 cut(s) 1259
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 1308
PfeI GAWTC 3 cut(s) 168, 518, 679
PflMI CCANNNNNTGG 4 cut(s) 713, 986, 1124, 1151
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 4 cut(s) 41, 44, 216, 278
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PpuMI RGGWCCY 2 cut(s) 1214, 1241
PsiI TTATAA 1 cut(s) 1374
Psp5II RGGWCCY 2 cut(s) 1214, 1241
Psp6I CCWGG 7 cut(s) 493, 766, 808, 832, 1117, 1144, 1258
PspGI CCWGG 7 cut(s) 493, 766, 808, 832, 1117, 1144, 1258
PspN4I GGNNCC 3 cut(s) 253, 1243, 1293
PspPPI RGGWCCY 2 cut(s) 1214, 1241
PstNI CAGNNNCTG 1 cut(s) 1349
PsuI RGATCY 3 cut(s) 1037, 1064, 1193
RsaI GTAC 2 cut(s) 492, 913
RsaNI GTAC 2 cut(s) 491, 912
RseI CAYNNNNRTG 2 cut(s) 302, 999
SaqAI TTAA 4 cut(s) 483, 801, 1365, 1423
SatI GCNGC 4 cut(s) 40, 43, 215, 277
Sau3AI GATC 9 cut(s) 179, 529, 631, 928, 1037, 1064, 1187, 1193, 1270
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 9 cut(s) 495, 768, 810, 834, 1025, 1026, 1119, 1146, 1260
SduI GDGCHC 4 cut(s) 620, 1117, 1144, 1231
SfcI CTRYAG 1 cut(s) 724
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 8 cut(s) 842, 1052, 1079, 1106, 1133, 1160, 1214, 1241
SmaI CCCGGG 1 cut(s) 1026
SmiMI CAYNNNNRTG 2 cut(s) 302, 999
SmlI CTYRAG 2 cut(s) 619, 1230
SmoI CTYRAG 2 cut(s) 619, 1230
SsiI CCGC 6 cut(s) 19, 64, 84, 141, 215, 590
SspMI CTAG 1 cut(s) 1224
StyD4I CCNGG 9 cut(s) 493, 766, 808, 832, 1023, 1024, 1117, 1144, 1258
StyI CCWWGG 3 cut(s) 1183, 1237, 1295
TaaI ACNGT 2 cut(s) 224, 629
TaiI ACGT 1 cut(s) 437
TaqI TCGA 3 cut(s) 178, 270, 1190
TaqII GACCGA 5 cut(s) 1069, 1096, 1123, 1150, 1177
TatI WGTACW 1 cut(s) 911
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 3 cut(s) 168, 518, 679
Tru1I TTAA 4 cut(s) 483, 801, 1365, 1423
Tru9I TTAA 4 cut(s) 483, 801, 1365, 1423
TscAI CASTG 3 cut(s) 296, 394, 1252
TseI GCWGC 3 cut(s) 39, 42, 276
TspDTI ATGAA 1 cut(s) 843
TspMI CCCGGG 1 cut(s) 1024
TspRI CASTG 3 cut(s) 296, 394, 1252
Van91I CCANNNNNTGG 4 cut(s) 713, 986, 1124, 1151
VpaK11BI GGWCC 8 cut(s) 842, 1052, 1079, 1106, 1133, 1160, 1214, 1241
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 2 cut(s) 411, 1378
XmaI CCCGGG 1 cut(s) 1024
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 1224
Zsp2I ATGCAT 1 cut(s) 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.