AT4G23460

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006717
Physical Location & Seq
Reverse (-)
12243671 .. 12249018
5348 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23460.2

Sequence Viewer

Length: 2682 bp
ATGAGCGGTCATGATTCTAAATATTTTTCGACTACGAAGAAGGGAGAGATCCCTGAGCTCAAGGAAGAGCTGAATTCACAGTACAAGGATAAGAGGAAAGATGCTGTTAAGAAGGTTATTGCGGCAATGACTGTTGGAAAGGATGTTTCATCACTTTTCACTGATGTAGTCAATTGCATGCAAACTGAGAATCTGGAGTTGAAGAAGCTTGTTTACTTGTATCTGATAAACTATGCAAAAAGCCAGCCAGATCTTGCTATCCTCGCTGTAAATACTTTTGTTAAGGATTCACAAGACCCAAATCCATTGATCCGGGCTTTGGCTGTGCGAACGATGGGCTGTATTCGTGTTGATAAGATTACAGAATATTTGTGTGATCCTCTTCAGAAATGCCTAAAGGATGATGATCCATATGTCCGCAAGACAGCAGCTATTTGTGTTGCTAAACTGTTTGACATAAATGCTGAATTAGTTGAGGATAGGGGGTTCCTGGAAGCCTTAAAGGATTTAATATCAGACAATAATCCAATGGTTGTGGCAAATGCTGTAGCTGCCCTTGCAGAGATCCAAGAGAATAGTACTAGTCCCATATTTGAAATCAATAGTACCATCCTCACAAAGCTCCTTACAGCTTTGAATGAATGTACTGAATGGGGTCAAGTTTTTATATTAGATGCTTTGTCGAGGTATAAGGCATCTGATCCTCGTGAAGCTGAAAATATTGTTGAGAGAGTCACTCCAAGGTTGCAACATGCCAACTGTGCTGTTGTGCTTTCAGCTGTTAAGATGATCCTTCAGCAGATGGAGCTTATTACTAGCACAGATGTGATTCGAAATCTTTGCAAGAAGATGGCTCCTCCCCTAGTTACATTGCTTTCTGCAGAACCTGAGATACAGTATGTTGCACTTCGTAACATTAACCTTATTGTCCAAAAGAGACCCACTATTCTTGCCCATGAAATCAAGGTGTTCTTCTGCAAGTATAATGATCCGATATATGTTAAGATGGAGAAGTTAGAGATTATGATAAAACTTGCTTCTGACAGAAACATAGACCAGGTTCTTCTGGAGTTCAAAGAATATGCCACGGAAGTAGATGTTGATTTTGTTCGGAAGGCTGTTCGTGCTATAGGCCGATGTGCCATCAAACTAGAAAGAGCAGCTGAACGGTGCATCAGTGTTTTACTTGAGCTGATCAAGATCAAAGTAAACTACGTTGTGCAGGAGGCCATTATAGTCATCAAAGATATCTTTAGAAGATATCCAAATACGTATGAGTCCATAATCGCAACACTCTGTGAGAGTCTAGACACATTGGATGAACCAGAAGCTAAGGCATCTATGATTTGGATCATTGGTGAATATGCTGAAAGAATCGACAATGCGGATGAACTTCTTGAAAGCTTCCTGGAGAATTTCCCTGAGGAACCAGCACAAGTCCAACTGCAGCTTCTTACAGCTACAGTCAAACTATTTCTGAAGAAGCCAACTGAAGGCCCACAACAAATGATTCAGGTTGTCTTGAATAATGCTACTGTTGAAACAGATAATCCTGATCTCAGGGATCGTGCATACATCTACTGGCGTCTTCTATCGACTGATCCTGAGGCGGCCAAGGATGTTGTTTTAGCTGAAAAGCCTGTGATTACTGATGACTCAAACCAACTTGATCCGTCGCTCCTGGATGAGCTACTTGCAAATATATCCACCTTGTCCTCTGTGTATCACAAGCCGCCAGAGGCTTTTGTAACCCGCTTGAAAACCACAGTTCAGAAAACGGAGGATGAGGATTATGTAGAAGGGAGCGAAACAGGATATCCCGAGGCATCTGGTAATCCGGTTGATGGTGCAGCTTCCCCGTCAGCTACCACAGGCTATGTCACAAAGCTAGCTGCTGCCCCAGCTCCTGTACCCGATCTGCTAGGTGACCTAATGGGGTCAGATAATGCTGCAATCGTCCCTGTAGATGAACCCACAACTCCGTCAGGTCGTCCACTGCCTGTTGTCCTACCAGCATCAAAGGGTCAAGGTCTGCAGATTAGCGCTCAATTAACCCGACAAGATGGTCAAGTATTCTACAGTATGCTCCTCGAGAACAACTCGCAGTCACTTCTTGATGGCTTCATGATTCAGTTCAACAAAAACTCATTCGGCCTTGCAGCTGTCGGATCACTTCAGGTTCCGCCTCTGCAACCCGGAGCATCTGCCAGGACAATGATGCCAATGGTATTGTCCCAAAACATGTCTACTGGCTCCACCAGCTCGGTATTGCAAGTTGCCGTCAAAAACAACCAGCAGCCTGTCTGGTACTTCGAGGATAAGATTGTACTTAACGCTCTTTTCTCAGAAGATGGTCGGATGGAGCGCGGAACCTTCCTCGAGACATGGAAATCGTTACCGGATTCAAACGAGGTGCAGAAAGAATTCCCCGGGATAACCATCACGAGCGTGGAGTCAACACTTGATTTACTCGCCGCATCAAACATGTTCTTCATAGCAAAGCGTAAGAATGGGAACCAAGACGTCCTCTATCTTTCGGCCAAGGTACCAAGAGGCATACCGTTCCTGATCGAACTAACGGCCATAGTGGGTCAACCTGGTCTGAAATGTGCAGTCAAGACTCCAACCCCTGAGATCGCTCCTCTCTTCTTTGAAGCAGTCGAAATTCTCTTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

893

Amino Acids

99.1

Weight (kDa)

4.91

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 2 - 440 8.4e-139 Adaptin N terminal region
HEAT_2 PF13646 4 - 56 4.7e-06 HEAT repeats
Cnd1 PF12717 9 - 172 2.7e-66 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 573 - 677 6e-11 Adaptin C-terminal domain
B2-adapt-app_C PF09066 688 - 796 3.7e-30 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2064
AatII GACGTC 1 cut(s) 2526
Acc65I GGTACC 1 cut(s) 2545
AccB1I GGYRCC 1 cut(s) 2545
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 2246
AccII CGCG 1 cut(s) 2367
AcoI YGGCCR 3 cut(s) 1611, 2538, 2580
AcsI RAATTY 4 cut(s) 73, 1414, 2423, 2664
AcuI CTGAAG 5 cut(s) 368, 779, 1499, 1512, 2159
AcyI GRCGYC 2 cut(s) 1585, 2523
AdeI CACNNNGTG 1 cut(s) 1298
AfaI GTAC 8 cut(s) 83, 580, 607, 646, 1911, 2309, 2328, 2547
AfeI AGCGCT 1 cut(s) 2044
AfiI CCNNNNNNNGG 3 cut(s) 319, 1493, 1844
AflIII ACRYGT 2 cut(s) 2241, 2484
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 6 cut(s) 489, 1056, 1407, 1680, 2207, 2596
AjuI GAANNNNNNNTTGG 2 cut(s) 1434, 1466
AleI CACNNNNGTG 2 cut(s) 824, 2447
AloI GAACNNNNNNTCC 2 cut(s) 470, 502
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 931, 2375
AlwNI CAGNNNCTG 2 cut(s) 887, 1907
Ama87I CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
Aor51HI AGCGCT 1 cut(s) 2044
AoxI GGCC 7 cut(s) 1132, 1227, 1495, 1611, 2152, 2538, 2580
ApoI RAATTY 4 cut(s) 73, 1414, 2423, 2664
Asp700I GAANNNNTTC 1 cut(s) 2423
Asp718I GGTACC 1 cut(s) 2545
AspLEI GCGC 2 cut(s) 2045, 2367
AspS9I GGNCC 1 cut(s) 1496
AsuC2I CCSGG 4 cut(s) 314, 2196, 2430, 2431
AsuHPI GGTGA 2 cut(s) 1370, 1937
AsuII TTCGAA 1 cut(s) 832
AsuNHI GCTAGC 1 cut(s) 1888
AvaI CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
AxyI CCTNAGG 2 cut(s) 1422, 1605
BanI GGYRCC 1 cut(s) 2545
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BauI CACGAG 2 cut(s) 705, 2443
BbsI GAAGAC 1 cut(s) 1580
Bbv12I GWGCWC 1 cut(s) 60
BceAI ACGGC 2 cut(s) 2264, 2595
BcgI CGANNNNNNTGC 2 cut(s) 822, 856
BciT130I CCWGG 6 cut(s) 491, 1058, 1409, 1682, 2209, 2598
BclI TGATCA 1 cut(s) 1194
BcnI CCSGG 4 cut(s) 314, 2196, 2430, 2431
BcoDI GTCTC 2 cut(s) 931, 2375
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 7 cut(s) 582, 816, 863, 1151, 1307, 1889, 1922
BfmI CTRYAG 8 cut(s) 546, 879, 1128, 1445, 1461, 1962, 2033, 2077
BfoI RGCGCY 1 cut(s) 2046
BglII AGATCT 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 580
BmeT110I CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
BmgT120I GGNCC 1 cut(s) 1496
BmiI GGNNCC 8 cut(s) 488, 855, 1428, 2181, 2254, 2371, 2516, 2547
BmtI GCTAGC 1 cut(s) 1892
BpiI GAAGAC 1 cut(s) 1580
BplI GAGNNNNNCTC 4 cut(s) 721, 753, 2084, 2116
BpmI CTGGAG 3 cut(s) 215, 1088, 1430
Bpu10I CCTNAGC 2 cut(s) 54, 1332
Bpu14I TTCGAA 1 cut(s) 832
BpuEI CTTGAG 2 cut(s) 44, 1208
BpuMI CCSGG 4 cut(s) 314, 2196, 2430, 2431
BsaAI YACGTR 1 cut(s) 1272
BsaBI GATNNNNATC 4 cut(s) 405, 1199, 1349, 2438
BsaHI GRCGYC 2 cut(s) 1585, 2523
BsaI GGTCTC 1 cut(s) 931
BsaJI CCNNGG 7 cut(s) 740, 1086, 1614, 1821, 2428, 2429, 2541
BsaWI WCCGGW 2 cut(s) 1837, 2398
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 1493, 1844
Bse1I ACTGG 2 cut(s) 1586, 2254
Bse21I CCTNAGG 2 cut(s) 1422, 1605
Bse3DI GCAATG 2 cut(s) 132, 869
Bse8I GATNNNNATC 4 cut(s) 405, 1199, 1349, 2438
BseBI CCWGG 6 cut(s) 491, 1058, 1409, 1682, 2209, 2598
BseDI CCNNGG 7 cut(s) 740, 1086, 1614, 1821, 2428, 2429, 2541
BseGI GGATG 9 cut(s) 148, 406, 609, 1324, 1393, 1624, 1690, 1789, 2364
BseJI GATNNNNATC 4 cut(s) 405, 1199, 1349, 2438
BseLI CCNNNNNNNGG 3 cut(s) 319, 1493, 1844
BseMI GCAATG 2 cut(s) 132, 869
BseMII CTCAG 8 cut(s) 45, 177, 879, 1413, 1573, 1596, 2358, 2622
BseNI ACTGG 2 cut(s) 1586, 2254
BseRI GAGGAG 3 cut(s) 846, 2078, 2631
BseYI CCCAGC 1 cut(s) 1900
BsgI GTGCAG 4 cut(s) 1241, 1869, 2435, 2631
Bsh1236I CGCG 1 cut(s) 2367
BshFI GGCC 7 cut(s) 1134, 1229, 1497, 1613, 2154, 2540, 2582
BshNI GGYRCC 1 cut(s) 2545
BsiHKAI GWGCWC 1 cut(s) 60
BsiHKCI CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
BsiSI CCGG 5 cut(s) 313, 1838, 2196, 2399, 2430
BslFI GGGAC 3 cut(s) 570, 1943, 2218
BslI CCNNNNNNNGG 3 cut(s) 319, 1493, 1844
BsmAI GTCTC 2 cut(s) 931, 2375
BsmFI GGGAC 3 cut(s) 570, 1943, 2218
BsnI GGCC 7 cut(s) 1134, 1229, 1497, 1613, 2154, 2540, 2582
Bso31I GGTCTC 1 cut(s) 931
BsoBI CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
Bsp119I TTCGAA 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 60
BspANI GGCC 7 cut(s) 1134, 1229, 1497, 1613, 2154, 2540, 2582
BspCNI CTCAG 8 cut(s) 46, 178, 880, 1414, 1572, 1597, 2357, 2623
BspFNI CGCG 1 cut(s) 2367
BspHI TCATGA 2 cut(s) 10, 2124
BspLI GGNNCC 8 cut(s) 488, 855, 1428, 2181, 2254, 2371, 2516, 2547
BspMAI CTGCAG 3 cut(s) 883, 1449, 2037
BspOI GCTAGC 1 cut(s) 1892
BspQI GCTCTTC 1 cut(s) 60
BspT104I TTCGAA 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 2545
BspTNI GGTCTC 1 cut(s) 931
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 132, 869
BsrI ACTGG 2 cut(s) 1586, 2254
BssECI CCNNGG 7 cut(s) 740, 1086, 1614, 1821, 2428, 2429, 2541
BssNI GRCGYC 2 cut(s) 1585, 2523
BssSI CACGAG 2 cut(s) 705, 2443
BssT1I CCWWGG 3 cut(s) 740, 1614, 2541
Bst2BI CACGAG 2 cut(s) 705, 2443
Bst2UI CCWGG 6 cut(s) 491, 1058, 1409, 1682, 2209, 2598
Bst6I CTCTTC 4 cut(s) 60, 387, 2651, 2675
BstACI GRCGYC 2 cut(s) 1585, 2523
BstBAI YACGTR 1 cut(s) 1272
BstBI TTCGAA 1 cut(s) 832
BstC8I GCNNGC 3 cut(s) 179, 245, 1890
BstDEI CTNAG 9 cut(s) 54, 186, 888, 1332, 1422, 1559, 1605, 2344, 2631
BstDSI CCRYGG 1 cut(s) 1086
BstEII GGTNACC 1 cut(s) 1925
BstF5I GGATG 9 cut(s) 148, 406, 609, 1324, 1393, 1624, 1690, 1789, 2364
BstFNI CGCG 1 cut(s) 2367
BstH2I RGCGCY 1 cut(s) 2046
BstHHI GCGC 2 cut(s) 2045, 2367
BstMAI GTCTC 2 cut(s) 931, 2375
BstMWI GCNNNNNNNGC 8 cut(s) 263, 551, 557, 761, 805, 1124, 1901, 2259
BstNI CCWGG 6 cut(s) 491, 1058, 1409, 1682, 2209, 2598
BstNSI RCATGY 4 cut(s) 181, 755, 2245, 2488
BstPI GGTNACC 1 cut(s) 1925
BstSFI CTRYAG 8 cut(s) 546, 879, 1128, 1445, 1461, 1962, 2033, 2077
BstSNI TACGTA 1 cut(s) 1272
BstUI CGCG 1 cut(s) 2367
BstV2I GAAGAC 1 cut(s) 1580
BstX2I RGATCY 3 cut(s) 48, 250, 564
BstYI RGATCY 3 cut(s) 48, 250, 564
Bsu36I CCTNAGG 2 cut(s) 1422, 1605
BsuRI GGCC 7 cut(s) 1134, 1229, 1497, 1613, 2154, 2540, 2582
BtgI CCRYGG 1 cut(s) 1086
BtsCI GGATG 9 cut(s) 148, 406, 609, 1324, 1393, 1624, 1690, 1789, 2364
BtsI GCAGTG 1 cut(s) 1994
BtsIMutI CAGTG 3 cut(s) 159, 1183, 1994
Cac8I GCNNGC 3 cut(s) 179, 245, 1890
CaiI CAGNNNCTG 2 cut(s) 887, 1907
CciI TCATGA 2 cut(s) 10, 2124
CfoI GCGC 2 cut(s) 2045, 2367
Cfr13I GGNCC 1 cut(s) 1496
Cfr9I CCCGGG 1 cut(s) 2429
CseI GACGC 1 cut(s) 1574
CsiI ACCWGGT 2 cut(s) 1056, 2596
Csp6I GTAC 8 cut(s) 82, 579, 606, 645, 1910, 2308, 2327, 2546
CspCI CAANNNNNGTGG 4 cut(s) 516, 551, 931, 966
CviAII CATG 8 cut(s) 11, 178, 752, 956, 2125, 2242, 2385, 2485
CviQI GTAC 8 cut(s) 82, 579, 606, 645, 1910, 2308, 2327, 2546
DdeI CTNAG 9 cut(s) 54, 186, 888, 1332, 1422, 1559, 1605, 2344, 2631
DraIII CACNNNGTG 1 cut(s) 1298
DrdI GACNNNNNNGTC 1 cut(s) 2064
DseDI GACNNNNNNGTC 1 cut(s) 2064
EaeI YGGCCR 3 cut(s) 1611, 2538, 2580
Eam1104I CTCTTC 4 cut(s) 60, 387, 2651, 2675
EarI CTCTTC 4 cut(s) 60, 387, 2651, 2675
EciI GGCGGA 1 cut(s) 2172
Ecl136II GAGCTC 1 cut(s) 58
Eco105I TACGTA 1 cut(s) 1272
Eco130I CCWWGG 3 cut(s) 740, 1614, 2541
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 931
Eco32I GATATC 3 cut(s) 1249, 1262, 1817
Eco47III AGCGCT 1 cut(s) 2044
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 5 cut(s) 368, 779, 1499, 1512, 2159
Eco81I CCTNAGG 2 cut(s) 1422, 1605
Eco88I CYCGRG 4 cut(s) 1820, 2090, 2378, 2429
Eco91I GGTNACC 1 cut(s) 1925
EcoICRI GAGCTC 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 1925
EcoRI GAATTC 2 cut(s) 73, 2423
EcoRII CCWGG 6 cut(s) 489, 1056, 1407, 1680, 2207, 2596
EcoRV GATATC 3 cut(s) 1249, 1262, 1817
EcoT14I CCWWGG 3 cut(s) 740, 1614, 2541
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 3 cut(s) 740, 1614, 2541
FaeI CATG 8 cut(s) 14, 181, 755, 959, 2128, 2245, 2388, 2488
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 3 cut(s) 570, 1943, 2218
FatI CATG 8 cut(s) 10, 177, 751, 955, 2124, 2241, 2384, 2484
FauI CCCGC 1 cut(s) 1760
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 2246
FokI GGATG 9 cut(s) 155, 413, 596, 1331, 1400, 1631, 1697, 1796, 2371
FriOI GRGCYC 1 cut(s) 60
FspBI CTAG 7 cut(s) 582, 816, 863, 1151, 1307, 1889, 1922
GlaI GCGC 2 cut(s) 2044, 2366
GsaI CCCAGC 1 cut(s) 1904
GsuI CTGGAG 3 cut(s) 215, 1088, 1430
HaeII RGCGCY 1 cut(s) 2046
HaeIII GGCC 7 cut(s) 1134, 1229, 1497, 1613, 2154, 2540, 2582
HapII CCGG 5 cut(s) 313, 1838, 2196, 2399, 2430
HgaI GACGC 1 cut(s) 1574
HhaI GCGC 2 cut(s) 2045, 2367
Hin1I GRCGYC 2 cut(s) 1585, 2523
Hin1II CATG 8 cut(s) 14, 181, 755, 959, 2128, 2245, 2388, 2488
Hin6I GCGC 2 cut(s) 2043, 2365
HinP1I GCGC 2 cut(s) 2043, 2365
HincII GTYRAC 2 cut(s) 2457, 2594
HindII GTYRAC 2 cut(s) 2457, 2594
HindIII AAGCTT 2 cut(s) 206, 1402
HpaII CCGG 5 cut(s) 313, 1838, 2196, 2399, 2430
HphI GGTGA 2 cut(s) 1370, 1937
Hpy166II GTNNAC 6 cut(s) 214, 1210, 1994, 2247, 2457, 2594
Hpy8I GTNNAC 6 cut(s) 214, 1210, 1994, 2247, 2457, 2594
Hpy99I CGWCG 1 cut(s) 1678
HpyAV CCTTC 7 cut(s) 34, 106, 803, 1108, 1487, 1793, 2383
HpyCH4IV ACGT 3 cut(s) 1215, 1271, 2523
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 551, 557, 761, 805, 1124, 1901, 2259
HpyF3I CTNAG 9 cut(s) 54, 186, 888, 1332, 1422, 1559, 1605, 2344, 2631
HpySE526I ACGT 3 cut(s) 1215, 1271, 2523
Hsp92I GRCGYC 2 cut(s) 1585, 2523
Hsp92II CATG 8 cut(s) 14, 181, 755, 959, 2128, 2245, 2388, 2488
HspAI GCGC 2 cut(s) 2043, 2365
KpnI GGTACC 1 cut(s) 2549
Ksp22I TGATCA 1 cut(s) 1194
LguI GCTCTTC 1 cut(s) 60
MabI ACCWGGT 2 cut(s) 1056, 2596
MaeI CTAG 7 cut(s) 582, 816, 863, 1151, 1307, 1889, 1922
MaeII ACGT 3 cut(s) 1215, 1271, 2523
MaeIII GTNAC 8 cut(s) 733, 865, 911, 1747, 1879, 1925, 2106, 2394
MbiI CCGCTC 1 cut(s) 6
MfeI CAATTG 1 cut(s) 172
MflI RGATCY 3 cut(s) 48, 250, 564
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 7 cut(s) 73, 172, 467, 1414, 2048, 2423, 2664
MlyI GAGTC 6 cut(s) 741, 1286, 1312, 1649, 2462, 2614
MmeI TCCRAC 5 cut(s) 115, 1465, 2146, 2336, 2648
MroXI GAANNNNTTC 1 cut(s) 2423
MseI TTAA 9 cut(s) 108, 282, 500, 509, 783, 918, 1002, 2051, 2331
MslI CAYNNNNRTG 2 cut(s) 824, 2447
MspA1I CMGCKG 3 cut(s) 779, 1163, 2162
MspI CCGG 5 cut(s) 313, 1838, 2196, 2399, 2430
MunI CAATTG 1 cut(s) 172
MvaI CCWGG 6 cut(s) 491, 1058, 1409, 1682, 2209, 2598
MvnI CGCG 1 cut(s) 2367
MwoI GCNNNNNNNGC 8 cut(s) 263, 551, 557, 761, 805, 1124, 1901, 2259
NciI CCSGG 4 cut(s) 314, 2196, 2430, 2431
NdeI CATATG 1 cut(s) 412
NheI GCTAGC 1 cut(s) 1888
NlaIII CATG 8 cut(s) 14, 181, 755, 959, 2128, 2245, 2388, 2488
NlaIV GGNNCC 8 cut(s) 488, 855, 1428, 2181, 2254, 2371, 2516, 2547
NmuCI GTSAC 4 cut(s) 733, 1879, 1925, 2106
NspI RCATGY 4 cut(s) 181, 755, 2245, 2488
NspV TTCGAA 1 cut(s) 832
OliI CACNNNNGTG 2 cut(s) 824, 2447
PaeI GCATGC 1 cut(s) 181
PaeR7I CTCGAG 2 cut(s) 2090, 2378
PagI TCATGA 2 cut(s) 10, 2124
PciI ACATGT 2 cut(s) 2241, 2484
PciSI GCTCTTC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 2423
PfeI GAWTC 8 cut(s) 14, 190, 287, 829, 1374, 1510, 2128, 2402
PfoI TCCNGGA 3 cut(s) 489, 1407, 1680
PleI GAGTC 6 cut(s) 740, 1285, 1311, 1649, 2461, 2614
PpsI GAGTC 6 cut(s) 740, 1285, 1311, 1649, 2461, 2614
Ppu21I YACGTR 1 cut(s) 1272
PscI ACATGT 2 cut(s) 2241, 2484
Psp124BI GAGCTC 1 cut(s) 60
Psp6I CCWGG 6 cut(s) 489, 1056, 1407, 1680, 2207, 2596
PspEI GGTNACC 1 cut(s) 1925
PspFI CCCAGC 1 cut(s) 1900
PspGI CCWGG 6 cut(s) 489, 1056, 1407, 1680, 2207, 2596
PspN4I GGNNCC 8 cut(s) 488, 855, 1428, 2181, 2254, 2371, 2516, 2547
PspPI GGNCC 1 cut(s) 1496
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstI CTGCAG 3 cut(s) 883, 1449, 2037
PstNI CAGNNNCTG 2 cut(s) 887, 1907
PsuI RGATCY 3 cut(s) 48, 250, 564
PvuII CAGCTG 3 cut(s) 779, 1163, 2162
RsaI GTAC 8 cut(s) 83, 580, 607, 646, 1911, 2309, 2328, 2547
RsaNI GTAC 8 cut(s) 82, 579, 606, 645, 1910, 2308, 2327, 2546
RseI CAYNNNNRTG 2 cut(s) 824, 2447
SacI GAGCTC 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 9 cut(s) 108, 282, 500, 509, 783, 918, 1002, 2051, 2331
Sau96I GGNCC 1 cut(s) 1496
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 6 cut(s) 741, 1286, 1312, 1649, 2462, 2614
SduI GDGCHC 1 cut(s) 60
SexAI ACCWGGT 2 cut(s) 1056, 2596
SfcI CTRYAG 8 cut(s) 546, 879, 1128, 1445, 1461, 1962, 2033, 2077
Sfr274I CTCGAG 2 cut(s) 2090, 2378
SfuI TTCGAA 1 cut(s) 832
SlaI CTCGAG 2 cut(s) 2090, 2378
SmaI CCCGGG 1 cut(s) 2431
SmiMI CAYNNNNRTG 2 cut(s) 824, 2447
SmlI CTYRAG 4 cut(s) 59, 1187, 2090, 2378
SmoI CTYRAG 4 cut(s) 59, 1187, 2090, 2378
SnaBI TACGTA 1 cut(s) 1272
SpeI ACTAGT 1 cut(s) 581
SphI GCATGC 1 cut(s) 181
Sse9I AATT 7 cut(s) 73, 172, 467, 1414, 2048, 2423, 2664
SspI AATATT 3 cut(s) 23, 368, 721
SspMI CTAG 7 cut(s) 582, 816, 863, 1151, 1307, 1889, 1922
SstI GAGCTC 1 cut(s) 60
StyI CCWWGG 3 cut(s) 740, 1614, 2541
TaiI ACGT 3 cut(s) 1218, 1274, 2526
TasI AATT 7 cut(s) 73, 172, 467, 1414, 2048, 2423, 2664
TatI WGTACW 4 cut(s) 81, 578, 644, 2326
TauI GCSGC 4 cut(s) 125, 1613, 1735, 2477
TfiI GAWTC 8 cut(s) 14, 190, 287, 829, 1374, 1510, 2128, 2402
Tru1I TTAA 9 cut(s) 108, 282, 500, 509, 783, 918, 1002, 2051, 2331
Tru9I TTAA 9 cut(s) 108, 282, 500, 509, 783, 918, 1002, 2051, 2331
TscAI CASTG 3 cut(s) 166, 1183, 2001
TseFI GTSAC 4 cut(s) 733, 1879, 1925, 2106
Tsp45I GTSAC 4 cut(s) 733, 1879, 1925, 2106
TspDTI ATGAA 8 cut(s) 138, 654, 972, 1335, 1404, 1983, 2113, 2482
TspGWI ACGGA 4 cut(s) 1103, 1662, 1793, 1971
TspMI CCCGGG 1 cut(s) 2429
TspRI CASTG 3 cut(s) 166, 1183, 2001
XapI RAATTY 4 cut(s) 73, 1414, 2423, 2664
XbaI TCTAGA 1 cut(s) 1306
XceI RCATGY 4 cut(s) 181, 755, 2245, 2488
XcmI CCANNNNNNNNNTGG 1 cut(s) 2446
XhoI CTCGAG 2 cut(s) 2090, 2378
XmaI CCCGGG 1 cut(s) 2429
XmiI GTMKAC 1 cut(s) 2246
XmnI GAANNNNTTC 1 cut(s) 2423
XspI CTAG 7 cut(s) 582, 816, 863, 1151, 1307, 1889, 1922
ZraI GACGTC 1 cut(s) 2524
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.