RchiOBHm_Chr7g0197091

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006717
Physical Location & Seq
Forward (+)
15027626 .. 15034461
6836 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17631

Sequence Viewer

Length: 2706 bp
ATGAGCGGGCACGACTCCAAGTACTTCTCCACCACCAAGAAGGGTGAAATTCCCGAGCTCAAAGAAGAGCTCAATTCTCAATACAAGGATAAGAGGAAAGATGCTGTTAAGAAGGTGATTGCTGCAATGACAGTTGGAAAGGATGTCTCGTCGCTTTTCACGGATGTAGTGAATTGCATGCAAACAGAAAATTTGGAGCTGAAGAAGCTGGTTTATTTGTATCTGATAAATTATGCTAAAAGCCAGCCTGACCTAGCAATTCTTGCTGTGAATACATTTGTCAAGGATTCACAGGACCCAAATCCTTTGATTCGCGCTTTAGCCGTTCGGACAATGGGATGCATTCGTGTTGATAAAATTACAGAATATCTATGTGATCCCCTTCAGAGATGCCTTAAGGATGATGATCCATATGTTCGCAAGACAGCAGCCATATGTGTCGCCAAACTTTACGACATAAATGCTGAGTTAGTTGAGGATAGGGGTTTCCTGGAATCTCTCAAGGATTTGATTTCTGATAATAATCCTATGGTTGTTGCAAATGCTGTGGCTGCGCTTGCTGAAATTCAAGAGAATAGTAATAGACCCATCTTTGAGATGACCAGTCACACTCTGACAAAGCTCCTTACTGCATTGAATGAATGCACAGAGTGGGGACAAGTATTTATACTGGATGCTCTTTCTAGATACAAGGCAGCTGATGCCCGTGAAGCTGAAAATATAGTAGAGCGAGTTACCCCGCGACTACAGCATGCTAACTGTGCTGTCGTGCTTTCAGCTGTAAAGATGATCCTCCAACAAATGGAACTTATCACCAGTACCGATGTTGTCAGAAATCTTTGCAAAAAGATGGCTCCTCCACTTGTGACACTACTCTCTGCTGAGCCTGAGATACAATATGTTGCATTAAGAAACATCAACCTTATAGTACAAAGACGGCCCACAATTCTTGCTCATGAAATTAAGGTATTCTTTTGCAAGTACAATGATCCTATTTATGTAAAGATGGAAAAGTTAGAAATTATGATAAAGCTTGCTTCAGATCGAAACATAGACCAGGTTCTATTGGAGTTTAAAGAGTATGCTACTGAAGTAGATGTTGATTTTGTCCGAAAGGCTGTCCGTGCCATTGGTCGCTGTGCCATCAAATTAGAAAGAGCAGCTGAACGGTGCATTACTGTATTACTTGAGTTGATTAAGATCAAAGTCAATTACGTAGTTCAAGAGGCCATTATTGTCATCAAAGATATCTTTAGAAGATATCCCAATACCTATGAATCCATCATTGCAACTCTATGCGAAAGCCTAGACACTTTAGATGAACCAGAAGCCAAGGCATCGATGATCTGGATAATTGGTGAATATGCGGAAAGAATTGACAATGCTGATGAGCTCCTTGAAAGCTTTCTGGAAAGTTTCCCCGAAGAACCTGCACATGTCCAATTGCAGTTGCTGACTGCAACTGTCAAACTTTTTCTTAAAAAGCCCACCGAAGGACCACAACAGATGATTCAGGTTGTTCTGAATAATGCTACTGTGGAGACAGACAATCCTGATCTGAGAGATCGTGCATACATATATTGGCGCCTTCTATCAACTGATCCTGAGGCAGCCAAGGATGTTGTTTTAGCTGAGAAGCCAGTGATCAGCGACGATTCAAACCTGCTTGATTCATCACTTCTGGATGAACTTCTTGCAAACATTGCTACGTTATCCTCTGTGTATCACAAGCCCCCAGAAGCGTTTGTTACCCGTGTGAAGACCTCATCGCCGAGACCTGAAGATGAGGAGTATGGTAGCGAAACAGGAAATTCTGAATCAGCAGCTGATAGTGCTGCTACTGGTGCATCCCCACCACCAACTAGTTCAGGTAGTGTTCCATATGCTGCACAAAGGCAACCAGTCCCTGCACCAGCTTCGCCTGCTGCTCCAGTGCCAGACTTACTTGGTGATCTGATTGGCCTGGATAACAGTGCCATTGTCCCTGTGGATCAGCCTGCTGCTCCTGCTGGACCTCCGTTGCCTGTCTTACTACCAGCATCAACTGGTCAAGGTTTACAAATCAGTGCTCAGTTGACACGCAGGGATGGTCAAATATTTTACAGTATATTGTTTGAGAACAACACACAGGTCCCATTAGATGGGTTTATGATTCAGTTCAACAAAAATACTTTCGGTCTTGCAGCTGCTGGACCCCTTCAGGTTCCACAAATTCAACCTGGGACATCAGCAGGGACTCTCCTGCCTATGGTTACGTTCCAGAATATGTCTCAAGGTCCTCCAAGCTCATTATTGCAGGTTGCTGTGAAAAACAATCAACAACCTGTCTGGTACTTCAATGATAAAATCCCATTGCATGTCTTCTTTACCGAGGATGGCAGAATGGAGCGTGCAAATTTTCTTGAGACTTGGAGATCCCTTCCTGATTCAAATGAGATTACAAAAGACTTCCCTGGAATTGTGGTGGGTAATGTTGAAGCAACTCTGGACCGCCTGGCTGCTACAAACATGTTCTTTATTGCGAAGCGAAAACACGCCAACCAGGATGTCTTCTATTTCTCTGCAAAGATACCTCGAGGAATACCCTTCCTAATTGAACTCACTACAGTAGTAAATACCCCGGGGGTCAAGTGCGCAATCAAGACTCCAAGCCCAGAATCAGCACCCCTTTTCTTTGAAGCTGTGGAGACACTCCTGAAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

901

Amino Acids

100.01

Weight (kDa)

5.05

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 14 - 534 1.5e-169 Adaptin N terminal region
HEAT_2 PF13646 98 - 152 2.6e-06 HEAT repeats
Cnd1 PF12717 103 - 266 2e-65 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 680 - 779 1.5e-11 Adaptin C-terminal domain
B2-adapt-app_C PF09066 790 - 899 2.1e-31 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2574
Acc16I TGCGCA 1 cut(s) 2635
Acc36I ACCTGC 3 cut(s) 1438, 1671, 2287
AccB1I GGYRCC 1 cut(s) 1584
AccB7I CCANNNNNTGG 2 cut(s) 802, 2141
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 2 cut(s) 315, 742
AciI CCGC 4 cut(s) 6, 740, 1367, 2491
AclWI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 1998, 2409
AcsI RAATTY 6 cut(s) 48, 190, 564, 1810, 2211, 2395
AcuI CTGAAG 6 cut(s) 221, 368, 1023, 1110, 1800, 2183
AcyI GRCGYC 1 cut(s) 1585
AdeI CACNNNGTG 1 cut(s) 651
AfaI GTAC 5 cut(s) 23, 820, 930, 983, 2333
AfiI CCNNNNNNNGG 4 cut(s) 802, 1493, 2141, 2248
AflII CTTAAG 1 cut(s) 395
AflIII ACRYGT 2 cut(s) 1435, 2508
AhlI ACTAGT 1 cut(s) 1862
AjnI CCWGG 7 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541
Alw21I GWGCWC 4 cut(s) 60, 72, 1395, 2071
Alw26I GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2681
AlwI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 1998, 2409
AlwNI CAGNNNCTG 4 cut(s) 1453, 1826, 1907, 2189
Ama87I CYCGRG 3 cut(s) 53, 2574, 2620
AoxI GGCC 3 cut(s) 938, 1227, 1960
ApoI RAATTY 6 cut(s) 48, 190, 564, 1810, 2211, 2395
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 4 cut(s) 317, 556, 1587, 2636
AspS9I GGNCC 8 cut(s) 295, 939, 1496, 2012, 2131, 2192, 2276, 2488
AsuC2I CCSGG 2 cut(s) 2621, 2622
AsuHPI GGTGA 5 cut(s) 56, 127, 805, 1370, 1961
AvaI CYCGRG 3 cut(s) 53, 2574, 2620
AvaII GGWCC 7 cut(s) 295, 1496, 2012, 2131, 2192, 2276, 2488
AxyI CCTNAGG 1 cut(s) 1605
BaeGI GKGCMC 1 cut(s) 12
BanI GGYRCC 1 cut(s) 1584
BanII GRGCYC 3 cut(s) 60, 72, 1395
BbsI GAAGAC 3 cut(s) 1766, 2353, 2542
Bbv12I GWGCWC 4 cut(s) 60, 72, 1395, 2071
BccI CCATC 8 cut(s) 596, 844, 1000, 1151, 1289, 2081, 2135, 2369
BceAI ACGGC 2 cut(s) 308, 953
BcgI CGANNNNNNTGC 6 cut(s) 443, 477, 1412, 1446, 1899, 1933
BciT130I CCWGG 7 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543
BclI TGATCA 1 cut(s) 1644
BcnI CCSGG 2 cut(s) 2621, 2622
BcoDI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2681
BcuI ACTAGT 1 cut(s) 1862
BfaI CTAG 4 cut(s) 254, 684, 1307, 1863
BfmI CTRYAG 2 cut(s) 746, 2604
BfoI RGCGCY 1 cut(s) 1588
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 3 cut(s) 1438, 1671, 2287
BlpI GCTNAGC 1 cut(s) 882
BmcAI AGTACT 1 cut(s) 23
Bme1390I CCNGG 9 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543, 2621, 2622
Bme18I GGWCC 7 cut(s) 295, 1496, 2012, 2131, 2192, 2276, 2488
BmeT110I CYCGRG 3 cut(s) 53, 2574, 2620
BmgT120I GGNCC 8 cut(s) 295, 939, 1496, 2012, 2131, 2192, 2276, 2488
BmiI GGNNCC 6 cut(s) 297, 855, 1586, 2133, 2194, 2205
BmrFI CCNGG 9 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543, 2621, 2622
BmsI GCATC 8 cut(s) 91, 329, 380, 664, 691, 1346, 1856, 2048
BpiI GAAGAC 3 cut(s) 1766, 2353, 2542
BpmI CTGGAG 1 cut(s) 1914
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 4 cut(s) 485, 1208, 2256, 2423
BpuMI CCSGG 2 cut(s) 2621, 2622
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 1 cut(s) 1216
BsaBI GATNNNNATC 3 cut(s) 405, 522, 1199
BsaHI GRCGYC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 1768
BsaJI CCNNGG 8 cut(s) 1332, 1614, 2219, 2370, 2452, 2619, 2620, 2621
Bsc4I CCNNNNNNNGG 4 cut(s) 802, 1493, 2141, 2248
Bse1I ACTGG 8 cut(s) 603, 675, 816, 1640, 1846, 1901, 1931, 2050
Bse21I CCTNAGG 1 cut(s) 1605
Bse3DI GCAATG 4 cut(s) 132, 1284, 1701, 2351
Bse8I GATNNNNATC 3 cut(s) 405, 522, 1199
BseBI CCWGG 7 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543
BseCI ATCGAT 1 cut(s) 1340
BseDI CCNNGG 8 cut(s) 1332, 1614, 2219, 2370, 2452, 2619, 2620, 2621
BseJI GATNNNNATC 3 cut(s) 405, 522, 1199
BseLI CCNNNNNNNGG 4 cut(s) 802, 1493, 2141, 2248
BseMI GCAATG 4 cut(s) 132, 1284, 1701, 2351
BseMII CTCAG 7 cut(s) 456, 873, 879, 1550, 1596, 1623, 2084
BseNI ACTGG 8 cut(s) 603, 675, 816, 1640, 1846, 1901, 1931, 2050
BseRI GAGGAG 2 cut(s) 846, 1802
BseSI GKGCMC 1 cut(s) 12
BsgI GTGCAG 3 cut(s) 1416, 1872, 1893
Bsh1236I CGCG 2 cut(s) 315, 742
BshFI GGCC 3 cut(s) 940, 1229, 1962
BshNI GGYRCC 1 cut(s) 1584
BshVI ATCGAT 1 cut(s) 1340
BsiHKAI GWGCWC 4 cut(s) 60, 72, 1395, 2071
BsiHKCI CYCGRG 3 cut(s) 53, 2574, 2620
BsiSI CCGG 1 cut(s) 2621
BslFI GGGAC 6 cut(s) 669, 1889, 1967, 2117, 2236, 2248
BslI CCNNNNNNNGG 4 cut(s) 802, 1493, 2141, 2248
BsmAI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2681
BsmFI GGGAC 6 cut(s) 669, 1889, 1967, 2117, 2236, 2248
BsmI GAATGC 2 cut(s) 342, 647
BsnI GGCC 3 cut(s) 940, 1229, 1962
Bso31I GGTCTC 1 cut(s) 1768
BsoBI CYCGRG 3 cut(s) 53, 2574, 2620
Bsp1286I GDGCHC 5 cut(s) 12, 60, 72, 1395, 2071
Bsp1720I GCTNAGC 1 cut(s) 882
BspACI CCGC 4 cut(s) 6, 740, 1367, 2491
BspANI GGCC 3 cut(s) 940, 1229, 1962
BspCNI CTCAG 7 cut(s) 457, 874, 880, 1551, 1597, 1624, 2083
BspDI ATCGAT 1 cut(s) 1340
BspFNI CGCG 2 cut(s) 315, 742
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 6 cut(s) 297, 855, 1586, 2133, 2194, 2205
BspMI ACCTGC 3 cut(s) 1438, 1671, 2287
BspPI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 1998, 2409
BspQI GCTCTTC 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 1584
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 1768
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 132, 1284, 1701, 2351
BsrI ACTGG 8 cut(s) 603, 675, 816, 1640, 1846, 1901, 1931, 2050
BssECI CCNNGG 8 cut(s) 1332, 1614, 2219, 2370, 2452, 2619, 2620, 2621
BssNI GRCGYC 1 cut(s) 1585
BssT1I CCWWGG 2 cut(s) 1332, 1614
Bst2UI CCWGG 7 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543
Bst4CI ACNGT 9 cut(s) 133, 761, 1170, 1180, 1465, 1537, 1973, 2105, 2608
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 1585
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 3 cut(s) 263, 701, 1703
BstBAI YACGTR 1 cut(s) 1216
BstC8I GCNNGC 9 cut(s) 8, 179, 245, 558, 753, 1035, 1923, 1998, 2391
BstDEI CTNAG 7 cut(s) 465, 882, 888, 1559, 1605, 1632, 2070
BstFNI CGCG 2 cut(s) 315, 742
BstH2I RGCGCY 1 cut(s) 1588
BstHHI GCGC 4 cut(s) 317, 556, 1587, 2636
BstMAI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2681
BstNI CCWGG 7 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543
BstNSI RCATGY 5 cut(s) 181, 755, 1439, 2360, 2512
BstSCI CCNGG 9 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541, 2619, 2620
BstSFI CTRYAG 2 cut(s) 746, 2604
BstSLI GKGCMC 1 cut(s) 12
BstSNI TACGTA 1 cut(s) 1216
BstUI CGCG 2 cut(s) 315, 742
BstV2I GAAGAC 3 cut(s) 1766, 2353, 2542
BstX2I RGATCY 1 cut(s) 2414
BstYI RGATCY 1 cut(s) 2414
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 1605
BsuRI GGCC 3 cut(s) 940, 1229, 1962
BsuTUI ATCGAT 1 cut(s) 1340
BtgZI GCGATG 1 cut(s) 1752
BtsIMutI CAGTG 4 cut(s) 1647, 1938, 1978, 2071
BveI ACCTGC 3 cut(s) 1438, 1671, 2287
Cac8I GCNNGC 9 cut(s) 8, 179, 245, 558, 753, 1035, 1923, 1998, 2391
CaiI CAGNNNCTG 4 cut(s) 1453, 1826, 1907, 2189
CciI TCATGA 1 cut(s) 955
CfoI GCGC 4 cut(s) 317, 556, 1587, 2636
Cfr13I GGNCC 8 cut(s) 295, 939, 1496, 2012, 2131, 2192, 2276, 2488
Cfr9I CCCGGG 1 cut(s) 2620
ClaI ATCGAT 1 cut(s) 1340
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 5 cut(s) 22, 819, 929, 982, 2332
CspCI CAANNNNNGTGG 4 cut(s) 528, 563, 931, 966
CviAII CATG 6 cut(s) 178, 752, 956, 1436, 2357, 2509
CviQI GTAC 5 cut(s) 22, 819, 929, 982, 2332
DdeI CTNAG 7 cut(s) 465, 882, 888, 1559, 1605, 1632, 2070
DinI GGCGCC 1 cut(s) 1586
DraI TTTAAA 1 cut(s) 1075
DraIII CACNNNGTG 1 cut(s) 651
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Ecl136II GAGCTC 3 cut(s) 58, 70, 1393
Eco105I TACGTA 1 cut(s) 1216
Eco130I CCWWGG 2 cut(s) 1332, 1614
Eco24I GRGCYC 3 cut(s) 60, 72, 1395
Eco31I GGTCTC 1 cut(s) 1768
Eco32I GATATC 2 cut(s) 1249, 1262
Eco47I GGWCC 7 cut(s) 295, 1496, 2012, 2131, 2192, 2276, 2488
Eco53kI GAGCTC 3 cut(s) 58, 70, 1393
Eco57I CTGAAG 6 cut(s) 221, 368, 1023, 1110, 1800, 2183
Eco81I CCTNAGG 1 cut(s) 1605
Eco88I CYCGRG 3 cut(s) 53, 2574, 2620
EcoICRI GAGCTC 3 cut(s) 58, 70, 1393
EcoO109I RGGNCCY 3 cut(s) 295, 2131, 2276
EcoRII CCWGG 7 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541
EcoRV GATATC 2 cut(s) 1249, 1262
EcoT14I CCWWGG 2 cut(s) 1332, 1614
EcoT22I ATGCAT 1 cut(s) 344
EcoT38I GRGCYC 3 cut(s) 60, 72, 1395
EgeI GGCGCC 1 cut(s) 1586
EheI GGCGCC 1 cut(s) 1586
ErhI CCWWGG 2 cut(s) 1332, 1614
FaeI CATG 6 cut(s) 181, 755, 959, 1439, 2360, 2512
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 6 cut(s) 669, 1889, 1967, 2117, 2236, 2248
FatI CATG 6 cut(s) 177, 751, 955, 1435, 2356, 2508
FauI CCCGC 1 cut(s) 747
FauNDI CATATG 3 cut(s) 412, 434, 1882
FbaI TGATCA 1 cut(s) 1644
FriOI GRGCYC 3 cut(s) 60, 72, 1395
FspBI CTAG 4 cut(s) 254, 684, 1307, 1863
FspI TGCGCA 1 cut(s) 2635
GlaI GCGC 4 cut(s) 316, 555, 1586, 2635
GsuI CTGGAG 1 cut(s) 1914
HaeII RGCGCY 1 cut(s) 1588
HaeIII GGCC 3 cut(s) 940, 1229, 1962
HapII CCGG 1 cut(s) 2621
HhaI GCGC 4 cut(s) 317, 556, 1587, 2636
Hin1I GRCGYC 1 cut(s) 1585
Hin1II CATG 6 cut(s) 181, 755, 959, 1439, 2360, 2512
Hin6I GCGC 4 cut(s) 315, 554, 1585, 2634
HinP1I GCGC 4 cut(s) 315, 554, 1585, 2634
HincII GTYRAC 1 cut(s) 2076
HindII GTYRAC 1 cut(s) 2076
HindIII AAGCTT 2 cut(s) 1031, 1402
HpaII CCGG 1 cut(s) 2621
HphI GGTGA 5 cut(s) 56, 127, 805, 1370, 1961
Hpy166II GTNNAC 2 cut(s) 2057, 2076
Hpy8I GTNNAC 2 cut(s) 2057, 2076
Hpy99I CGWCG 2 cut(s) 154, 1655
HpyAV CCTTC 9 cut(s) 34, 106, 392, 1487, 1598, 2207, 2429, 2596, 2692
HpyCH4III ACNGT 9 cut(s) 133, 761, 1170, 1180, 1465, 1537, 1973, 2105, 2608
HpyCH4IV ACGT 3 cut(s) 1215, 1709, 2255
HpyF3I CTNAG 7 cut(s) 465, 882, 888, 1559, 1605, 1632, 2070
HpySE526I ACGT 3 cut(s) 1215, 1709, 2255
Hsp92I GRCGYC 1 cut(s) 1585
Hsp92II CATG 6 cut(s) 181, 755, 959, 1439, 2360, 2512
HspAI GCGC 4 cut(s) 315, 554, 1585, 2634
KasI GGCGCC 1 cut(s) 1584
Ksp22I TGATCA 1 cut(s) 1644
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 7 cut(s) 196, 627, 859, 1398, 1933, 2008, 2386
LweI GCATC 8 cut(s) 91, 329, 380, 664, 691, 1346, 1856, 2048
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 4 cut(s) 254, 684, 1307, 1863
MaeII ACGT 3 cut(s) 1215, 1709, 2255
MaeIII GTNAC 5 cut(s) 605, 733, 865, 1747, 2251
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 8 cut(s) 77, 214, 1269, 1436, 1771, 1793, 2353, 2542
MfeI CAATTG 1 cut(s) 1442
MflI RGATCY 1 cut(s) 2414
MhlI GDGCHC 5 cut(s) 12, 60, 72, 1395, 2071
Mly113I GGCGCC 1 cut(s) 1585
MlyI GAGTC 3 cut(s) 8, 2230, 2638
MmeI TCCRAC 2 cut(s) 115, 820
Mph1103I ATGCAT 1 cut(s) 344
MroXI GAANNNNTTC 1 cut(s) 1404
MseI TTAA 7 cut(s) 108, 396, 908, 963, 1074, 1197, 1479
MspA1I CMGCKG 5 cut(s) 698, 779, 1163, 1826, 2186
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 1 cut(s) 2621
MspR9I CCNGG 9 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543, 2621, 2622
MunI CAATTG 1 cut(s) 1442
Mva1269I GAATGC 2 cut(s) 342, 647
MvaI CCWGG 7 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543
MvnI CGCG 2 cut(s) 315, 742
NarI GGCGCC 1 cut(s) 1585
NciI CCSGG 2 cut(s) 2621, 2622
NdeI CATATG 3 cut(s) 412, 434, 1882
NlaIII CATG 6 cut(s) 181, 755, 959, 1439, 2360, 2512
NlaIV GGNNCC 6 cut(s) 297, 855, 1586, 2133, 2194, 2205
NmeAIII GCCGAG 1 cut(s) 1797
NmuCI GTSAC 2 cut(s) 605, 865
NsbI TGCGCA 1 cut(s) 2635
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 5 cut(s) 181, 755, 1439, 2360, 2512
PaeI GCATGC 2 cut(s) 181, 755
PaeR7I CTCGAG 1 cut(s) 2574
PagI TCATGA 1 cut(s) 955
PciI ACATGT 2 cut(s) 1435, 2508
PciSI GCTCTTC 1 cut(s) 60
PctI GAATGC 2 cut(s) 342, 647
PdmI GAANNNNTTC 1 cut(s) 1404
PflMI CCANNNNNTGG 2 cut(s) 802, 2141
PfoI TCCNGGA 1 cut(s) 489
PleI GAGTC 3 cut(s) 8, 2230, 2638
PluTI GGCGCC 1 cut(s) 1588
PpsI GAGTC 3 cut(s) 8, 2230, 2638
Ppu21I YACGTR 1 cut(s) 1216
PpuMI RGGWCCY 3 cut(s) 295, 2131, 2276
PscI ACATGT 2 cut(s) 1435, 2508
Psp124BI GAGCTC 3 cut(s) 60, 72, 1395
Psp5II RGGWCCY 3 cut(s) 295, 2131, 2276
Psp6I CCWGG 7 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541
PspGI CCWGG 7 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541
PspN4I GGNNCC 6 cut(s) 297, 855, 1586, 2133, 2194, 2205
PspPI GGNCC 8 cut(s) 295, 939, 1496, 2012, 2131, 2192, 2276, 2488
PspPPI RGGWCCY 3 cut(s) 295, 2131, 2276
PspXI VCTCGAGB 1 cut(s) 2574
PstNI CAGNNNCTG 4 cut(s) 1453, 1826, 1907, 2189
PsuI RGATCY 1 cut(s) 2414
PvuII CAGCTG 5 cut(s) 698, 779, 1163, 1826, 2186
RsaI GTAC 5 cut(s) 23, 820, 930, 983, 2333
RsaNI GTAC 5 cut(s) 22, 819, 929, 982, 2332
SacI GAGCTC 3 cut(s) 60, 72, 1395
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 7 cut(s) 108, 396, 908, 963, 1074, 1197, 1479
Sau96I GGNCC 8 cut(s) 295, 939, 1496, 2012, 2131, 2192, 2276, 2488
ScaI AGTACT 1 cut(s) 23
SchI GAGTC 3 cut(s) 8, 2230, 2638
ScrFI CCNGG 9 cut(s) 491, 1058, 1964, 2220, 2454, 2495, 2543, 2621, 2622
SduI GDGCHC 5 cut(s) 12, 60, 72, 1395, 2071
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 8 cut(s) 91, 329, 380, 664, 691, 1346, 1856, 2048
SfcI CTRYAG 2 cut(s) 746, 2604
SfoI GGCGCC 1 cut(s) 1586
Sfr274I CTCGAG 1 cut(s) 2574
SinI GGWCC 7 cut(s) 295, 1496, 2012, 2131, 2192, 2276, 2488
SlaI CTCGAG 1 cut(s) 2574
SmaI CCCGGG 1 cut(s) 2622
SmlI CTYRAG 6 cut(s) 395, 500, 1187, 2271, 2402, 2574
SmoI CTYRAG 6 cut(s) 395, 500, 1187, 2271, 2402, 2574
SnaBI TACGTA 1 cut(s) 1216
SpeI ACTAGT 1 cut(s) 1862
SphI GCATGC 2 cut(s) 181, 755
SsiI CCGC 4 cut(s) 6, 740, 1367, 2491
SspDI GGCGCC 1 cut(s) 1584
SspI AATATT 1 cut(s) 2097
SspMI CTAG 4 cut(s) 254, 684, 1307, 1863
SstI GAGCTC 3 cut(s) 60, 72, 1395
StyD4I CCNGG 9 cut(s) 489, 1056, 1962, 2218, 2452, 2493, 2541, 2619, 2620
StyI CCWWGG 2 cut(s) 1332, 1614
TaaI ACNGT 9 cut(s) 133, 761, 1170, 1180, 1465, 1537, 1973, 2105, 2608
TaiI ACGT 3 cut(s) 1218, 1712, 2258
TaqI TCGA 3 cut(s) 1045, 1340, 2575
TaqII GACCGA 1 cut(s) 2165
TatI WGTACW 3 cut(s) 21, 928, 981
Tru1I TTAA 7 cut(s) 108, 396, 908, 963, 1074, 1197, 1479
Tru9I TTAA 7 cut(s) 108, 396, 908, 963, 1074, 1197, 1479
TscAI CASTG 4 cut(s) 1647, 1938, 1978, 2071
TseFI GTSAC 2 cut(s) 605, 865
Tsp45I GTSAC 2 cut(s) 605, 865
TspDTI ATGAA 6 cut(s) 654, 972, 1290, 1335, 1662, 1701
TspGWI ACGGA 3 cut(s) 176, 1112, 2007
TspMI CCCGGG 1 cut(s) 2620
TspRI CASTG 4 cut(s) 1647, 1938, 1978, 2071
Van91I CCANNNNNTGG 2 cut(s) 802, 2141
Vha464I CTTAAG 1 cut(s) 395
VpaK11BI GGWCC 7 cut(s) 295, 1496, 2012, 2131, 2192, 2276, 2488
XapI RAATTY 6 cut(s) 48, 190, 564, 1810, 2211, 2395
XbaI TCTAGA 1 cut(s) 683
XceI RCATGY 5 cut(s) 181, 755, 1439, 2360, 2512
XcmI CCANNNNNNNNNTGG 1 cut(s) 1984
XhoI CTCGAG 1 cut(s) 2574
XmaI CCCGGG 1 cut(s) 2620
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 4 cut(s) 254, 684, 1307, 1863
ZrmI AGTACT 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.