MD14G1108600.v1.1

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006717
Physical Location & Seq
Reverse (-)
16922900 .. 16930198
7299 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1108600.v1.1.491

Sequence Viewer

Length: 2709 bp
ATGAGCGGTCACGACTCCAAGTACTTCTCCACCACCAAGAAGGGCGAAATCCCTGAGCTCAAAGAGGAGCTCAATTCTCAATACAAGGATAAGAGAAAAGATGCCGTCAAGAAGGTGATCGCAGCAATGACTGTTGGGAAGGATGTTTCATCACTTTTCACAGATGTAGTAAATTGCATGCAAACAGAAAACTTGGAGCTGAAGAAGCTAGTCTATTTGTATCTGATAAATTATGCTAAAAGCCAGCCTGACCTAGCTATACTTGCGGTGAATACATTTGTCAAGGATTCACAGGACCCAAATCCTTTGATTCGTGCTTTAGCCGTCCGGACAATGGGTTGTATTCGTGTTGATAAAATTACCGAATATCTATGTGATCCCCTTCAGAGGTGTCTTAAGGATGATGATCCATATGTTCGGAAGACAGCAGCCATATGTGTGGCCAAACTTTATGACATAAATGCAGAGTTAGTTGAGGATAGGGGTTTTCTGGAATCTCTCAAGGATTTGATATCTGATAATAATCCTATGGTTGTAGCCAATGCTGTGGCTGCTCTAGCCGAAATTCAAGAGAATAGTACTAGACCCATCTTTGAGATCACTAGTCACACTCTGTCAAAGCTCCTAACTGCCTTAAATGAATGCACAGAGTGGGGTCAAGTTTTCATATTGGACGCTCTTTCTAAATACAAGGCAGCAGACGCTCGTGAAGCTGAAAATATAGTAGAGCGAGTTACACCACGATTACAACACGCTAACTGTGCGGTTGTACTTTCAGCTGTTAAGATGATCCTCCAACAAATGGAACTTATAACTAGTACTGATGTCGTCAGAAATCTTTGCAAAAAGATGGCTCCTCCACTCGTGACACTGTTATCTGCTGAGCCTGAGATACAATATGTTGCATTGAGAAATATCAACCTTATAGTACAAAGACGACCCACAATCCTTGCTCATGAAATTAAGGTGTTCTTCTGCAAGTACAATGATCCAATTTATGTAAAGATGGAAAAGTTAGAAATCATGATCAAGCTTGCTTCAGACCGAAATATAGACCAGGTTTTGTTGGAGTTTAAAGAGTATGCTACGGAAGTAGATGTGGATTTTGTCCGAAAAGCTGTCCGCGCCATTGGTCGCTGTGCAATCAAATTAGAAAGAGCAGCTGAGCGGTGCATTACTGTTTTACTGGAGCTTATTAAGATCAAAGTAAATTATGTAGTTCAAGAGGCCATTATTGTCATCAAAGATATATTTAGAAGATATCCAAATACCTATGAGTCCATTATTGCCACTCTGTGCGAAAGCCTTGACACTTTAGATGAACCAGAAGCCAAGGCATCAATGATCTGGATAATTGGTGAATATGCAGAACGAATTGACAATGCTGATGAGCTACTTGAAAGTTTTCTGGAAAGTTTCCCTGAAGAGCCTGCACATGTCCAATTGCAATTGCTAACTGCAACTGTCAAACTCTTTCTTAAGAAGCCGACTGAAGGACCACAGCAGATGATACAGGTTGTTTTAAATAATGCTACTGTGGAAACAGACAATCCTGATTTGCGAGATCGGGCCTACATATATTGGCGTCTTTTATCAACTGACCCAGAGGCAGCCAAGGATGTGGTGTTAGCTGAGAAGCCAGTGATCAGTGACGATTCAAACCTGATTGATCCATCTCTTCTTGATGAGCTTCTTGCTAACATTGCTACGTTATCCTCTGTGTATCACAAGCCTCCAGAAGCATTTGTTACCCGTGTGAAGGCCACAGCCCAAAGAACTGAAGATGAGGACTATGGTAGTGAAACAGGAAATTCTGAATCACCTGCTCATGTTGCTGATAGCTCTGCATCCCCAACCGCAACTAGTTCAGGTGTTCCATATGCTGCAGCTAGACAACCTACACCAGTTTCGCCTGCACCTGCACCTGCTGCTCCAGTACCAGATTTGCTTGGTGACCTGATGGGCCTGGAAAACAGTGCTATTGTCCCTGTGGATCAACCTGCTTCTCCCGCTGGACCTCCGTTGCCTGTCGTTCTCCCAGCGTCAACTGGTCAAGGTTTACAAATCAGTGCTCAGTTGACATGTAGGGAAGGACAAATATTTTACAGTTTATTGTTCGAGAACAACACACAGGTTCCACTAGATGGGTTCATGATTCAGTTCAACAAAAATACATTTGGTCTTGCAGCTGCTGGACCCCTTCAGGTTCCACAATTGCAACCTGGGAAATCAGCGGGGACTCTCTTGCCTATGGTTACGTTCCAGAATATGTCTCAAGGTCCTCCAAGCTCACTATTGCAGGTTGCTGTGAAAAACAATCAACAACCAGTCTGGTACTTTAACGATAAAATCTCATTGCACGTCTTCTTTACTGATGATGGAAGAATGGAGCGTGCAAACTTTCTTGAGACATGGAGGTCTCTTCCGGACACAAATGAGATTACAAAGGACTTACCTGGAATTGTGGTGAGCAATGTTGAGGCGACTCTGGACCGCCTGGCTGCTACAAACATGTTCTTCATTGCGAAACGCAAACATGCCAACCAGGACGTGTTCTATTTCTCAGCAAAAATCCCTCGAGGAATACCGTTCTTGATTGAACTCACCACTGTCGTGAATAACCCCGGGGTCAAGATTGCAATCAAGACTCCAAGCCCGGAGACAGCACCTCTTTTCTTTGAAGCCATGGAGACTCTCCTCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

903

Amino Acids

100.16

Weight (kDa)

5.07

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 14 - 534 2.2e-169 Adaptin N terminal region
HEAT_2 PF13646 98 - 187 8.9e-09 HEAT repeats
Cnd1 PF12717 103 - 266 3.5e-66 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 676 - 780 1.2e-10 Adaptin C-terminal domain
B2-adapt-app_C PF09066 792 - 900 8.2e-31 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
AarI CACCTGC 3 cut(s) 1831, 1927, 1933
AasI GACNNNNNNGTC 1 cut(s) 2416
AbsI CCTCGAGG 1 cut(s) 2577
Acc36I ACCTGC 5 cut(s) 1831, 1927, 1933, 2008, 2290
AccB7I CCANNNNNTGG 2 cut(s) 802, 2144
AccBSI CCGCTC 2 cut(s) 6, 1168
AccII CGCG 1 cut(s) 1125
AccIII TCCGGA 2 cut(s) 327, 2425
AciI CCGC 9 cut(s) 6, 266, 764, 1123, 1168, 1857, 2010, 2234, 2494
AclWI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2001
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 2 cut(s) 564, 1810
AcuI CTGAAG 7 cut(s) 221, 368, 1023, 1443, 1512, 1800, 2186
AcyI GRCGYC 1 cut(s) 1585
AdeI CACNNNGTG 2 cut(s) 651, 1296
AfaI GTAC 8 cut(s) 23, 580, 771, 820, 930, 983, 1938, 2336
AfiI CCNNNNNNNGG 6 cut(s) 334, 387, 802, 1493, 1759, 2144
AflII CTTAAG 2 cut(s) 395, 1478
AflIII ACRYGT 4 cut(s) 1435, 2081, 2511, 2550
AgsI TTSAA 7 cut(s) 569, 1223, 1400, 1659, 2164, 2600, 2681
AhlI ACTAGT 3 cut(s) 602, 815, 1862
AjiI CACGTC 2 cut(s) 2362, 2551
AjnI CCWGG 6 cut(s) 1056, 1965, 2221, 2455, 2496, 2544
AleI CACNNNNGTG 1 cut(s) 2612
Alw21I GWGCWC 3 cut(s) 60, 72, 2074
Alw26I GTCTC 5 cut(s) 2277, 2402, 2424, 2654, 2684
AlwI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2001
AlwNI CAGNNNCTG 1 cut(s) 2192
Ama87I CYCGRG 2 cut(s) 2577, 2624
Aor13HI TCCGGA 2 cut(s) 327, 2425
AoxI GGCC 5 cut(s) 441, 1227, 1569, 1761, 1963
ApoI RAATTY 2 cut(s) 564, 1810
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 1127
AspS9I GGNCC 8 cut(s) 295, 1496, 1569, 1963, 2015, 2195, 2279, 2491
AsuC2I CCSGG 3 cut(s) 2625, 2626, 2657
AsuHPI GGTGA 7 cut(s) 127, 280, 1370, 1812, 1964, 2479, 2596
AvaI CYCGRG 2 cut(s) 2577, 2624
AvaII GGWCC 6 cut(s) 295, 1496, 2015, 2195, 2279, 2491
BalI TGGCCA 1 cut(s) 443
BanII GRGCYC 2 cut(s) 60, 72
BauI CACGAG 2 cut(s) 705, 863
BbsI GAAGAC 2 cut(s) 428, 2356
Bbv12I GWGCWC 3 cut(s) 60, 72, 2074
BccI CCATC 7 cut(s) 596, 844, 1000, 1681, 1954, 2138, 2372
BceAI ACGGC 2 cut(s) 89, 308
BciT130I CCWGG 6 cut(s) 1058, 1967, 2223, 2457, 2498, 2546
BclI TGATCA 2 cut(s) 1026, 1644
BcnI CCSGG 3 cut(s) 2625, 2626, 2657
BcoDI GTCTC 5 cut(s) 2277, 2402, 2424, 2654, 2684
BcuI ACTAGT 3 cut(s) 602, 815, 1862
BfaI CTAG 9 cut(s) 209, 254, 557, 582, 603, 816, 1863, 1890, 2141
BfmI CTRYAG 1 cut(s) 1884
BfrI CTTAAG 2 cut(s) 395, 1478
BfuAI ACCTGC 5 cut(s) 1831, 1927, 1933, 2008, 2290
BlpI GCTNAGC 2 cut(s) 882, 1164
BmcAI AGTACT 3 cut(s) 23, 580, 820
Bme1390I CCNGG 9 cut(s) 1058, 1967, 2223, 2457, 2498, 2546, 2625, 2626, 2657
Bme18I GGWCC 6 cut(s) 295, 1496, 2015, 2195, 2279, 2491
BmeT110I CYCGRG 2 cut(s) 2577, 2624
BmgBI CACGTC 2 cut(s) 2362, 2551
BmgT120I GGNCC 8 cut(s) 295, 1496, 1569, 1963, 2015, 2195, 2279, 2491
BmiI GGNNCC 5 cut(s) 297, 855, 2136, 2197, 2208
BmrFI CCNGG 9 cut(s) 1058, 1967, 2223, 2457, 2498, 2546, 2625, 2626, 2657
BmsI GCATC 3 cut(s) 91, 1346, 1856
BpiI GAAGAC 2 cut(s) 428, 2356
BpmI CTGGAG 3 cut(s) 1208, 1719, 1917
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 2 cut(s) 882, 1164
BpuEI CTTGAG 4 cut(s) 485, 2259, 2426, 2684
BpuMI CCSGG 3 cut(s) 2625, 2626, 2657
BsaBI GATNNNNATC 3 cut(s) 405, 522, 2639
BsaHI GRCGYC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 2424
BsaJI CCNNGG 7 cut(s) 1332, 1614, 2222, 2623, 2624, 2625, 2685
BsaWI WCCGGW 2 cut(s) 327, 2425
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 387, 802, 1493, 1759, 2144
Bse1I ACTGG 6 cut(s) 1191, 1640, 1904, 1934, 2053, 2327
Bse3DI GCAATG 5 cut(s) 132, 1701, 2354, 2479, 2520
Bse8I GATNNNNATC 3 cut(s) 405, 522, 2639
BseAI TCCGGA 2 cut(s) 327, 2425
BseBI CCWGG 6 cut(s) 1058, 1967, 2223, 2457, 2498, 2546
BseDI CCNNGG 7 cut(s) 1332, 1614, 2222, 2623, 2624, 2625, 2685
BseGI GGATG 4 cut(s) 148, 406, 1624, 1847
BseJI GATNNNNATC 3 cut(s) 405, 522, 2639
BseLI CCNNNNNNNGG 6 cut(s) 334, 387, 802, 1493, 1759, 2144
BseMI GCAATG 5 cut(s) 132, 1701, 2354, 2479, 2520
BseMII CTCAG 7 cut(s) 45, 873, 879, 1155, 1623, 2087, 2577
BseNI ACTGG 6 cut(s) 1191, 1640, 1904, 1934, 2053, 2327
BseRI GAGGAG 3 cut(s) 80, 846, 2687
BseYI CCCAGC 1 cut(s) 2038
BsgI GTGCAG 3 cut(s) 1416, 1899, 1905
Bsh1236I CGCG 1 cut(s) 1125
BshFI GGCC 5 cut(s) 443, 1229, 1571, 1763, 1965
BsiHKAI GWGCWC 3 cut(s) 60, 72, 2074
BsiHKCI CYCGRG 2 cut(s) 2577, 2624
BsiSI CCGG 4 cut(s) 328, 2426, 2625, 2657
BslFI GGGAC 2 cut(s) 1970, 2251
BslI CCNNNNNNNGG 6 cut(s) 334, 387, 802, 1493, 1759, 2144
BsmAI GTCTC 5 cut(s) 2277, 2402, 2424, 2654, 2684
BsmFI GGGAC 2 cut(s) 1970, 2251
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 5 cut(s) 443, 1229, 1571, 1763, 1965
Bso31I GGTCTC 1 cut(s) 2424
BsoBI CYCGRG 2 cut(s) 2577, 2624
Bsp1286I GDGCHC 3 cut(s) 60, 72, 2074
Bsp13I TCCGGA 2 cut(s) 327, 2425
Bsp1720I GCTNAGC 2 cut(s) 882, 1164
Bsp19I CCATGG 1 cut(s) 2685
BspACI CCGC 9 cut(s) 6, 266, 764, 1123, 1168, 1857, 2010, 2234, 2494
BspANI GGCC 5 cut(s) 443, 1229, 1571, 1763, 1965
BspCNI CTCAG 7 cut(s) 46, 874, 880, 1156, 1624, 2086, 2576
BspEI TCCGGA 2 cut(s) 327, 2425
BspFNI CGCG 1 cut(s) 1125
BspHI TCATGA 3 cut(s) 955, 1023, 2151
BspLI GGNNCC 5 cut(s) 297, 855, 2136, 2197, 2208
BspMAI CTGCAG 1 cut(s) 1888
BspMI ACCTGC 5 cut(s) 1831, 1927, 1933, 2008, 2290
BspPI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2001
BspQI GCTCTTC 1 cut(s) 1419
BspTI CTTAAG 2 cut(s) 395, 1478
BspTNI GGTCTC 1 cut(s) 2424
BsrBI CCGCTC 2 cut(s) 6, 1168
BsrDI GCAATG 5 cut(s) 132, 1701, 2354, 2479, 2520
BsrI ACTGG 6 cut(s) 1191, 1640, 1904, 1934, 2053, 2327
BssECI CCNNGG 7 cut(s) 1332, 1614, 2222, 2623, 2624, 2625, 2685
BssNI GRCGYC 1 cut(s) 1585
BssSI CACGAG 2 cut(s) 705, 863
BssT1I CCWWGG 3 cut(s) 1332, 1614, 2685
Bst2BI CACGAG 2 cut(s) 705, 863
Bst2UI CCWGG 6 cut(s) 1058, 1967, 2223, 2457, 2498, 2546
Bst6I CTCTTC 3 cut(s) 1419, 1683, 2427
BstACI GRCGYC 1 cut(s) 1585
BstAFI CTTAAG 2 cut(s) 395, 1478
BstAPI GCANNNNNTGC 1 cut(s) 1928
BstC8I GCNNGC 6 cut(s) 179, 245, 1035, 1431, 1914, 2394
BstDEI CTNAG 7 cut(s) 54, 882, 888, 1164, 1632, 2073, 2563
BstDSI CCRYGG 1 cut(s) 2685
BstEII GGTNACC 1 cut(s) 1952
BstF5I GGATG 4 cut(s) 148, 406, 1624, 1847
BstFNI CGCG 1 cut(s) 1125
BstHHI GCGC 1 cut(s) 1127
BstMAI GTCTC 5 cut(s) 2277, 2402, 2424, 2654, 2684
BstNI CCWGG 6 cut(s) 1058, 1967, 2223, 2457, 2498, 2546
BstNSI RCATGY 5 cut(s) 181, 1439, 2085, 2515, 2540
BstPI GGTNACC 1 cut(s) 1952
BstSCI CCNGG 9 cut(s) 1056, 1965, 2221, 2455, 2496, 2544, 2623, 2624, 2655
BstSFI CTRYAG 1 cut(s) 1884
BstUI CGCG 1 cut(s) 1125
BstV2I GAAGAC 2 cut(s) 428, 2356
BstXI CCANNNNNNTGG 3 cut(s) 439, 547, 1621
BsuRI GGCC 5 cut(s) 443, 1229, 1571, 1763, 1965
BtgI CCRYGG 1 cut(s) 2685
BtrI CACGTC 2 cut(s) 2362, 2551
BtsCI GGATG 4 cut(s) 148, 406, 1624, 1847
BtsIMutI CAGTG 6 cut(s) 869, 1647, 1654, 1981, 2074, 2607
BveI ACCTGC 5 cut(s) 1831, 1927, 1933, 2008, 2290
Cac8I GCNNGC 6 cut(s) 179, 245, 1035, 1431, 1914, 2394
CaiI CAGNNNCTG 1 cut(s) 2192
CciI TCATGA 3 cut(s) 955, 1023, 2151
CfoI GCGC 1 cut(s) 1127
Cfr13I GGNCC 8 cut(s) 295, 1496, 1569, 1963, 2015, 2195, 2279, 2491
Cfr9I CCCGGG 1 cut(s) 2624
CseI GACGC 4 cut(s) 683, 710, 1574, 2031
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 8 cut(s) 22, 579, 770, 819, 929, 982, 1937, 2335
CspCI CAANNNNNGTGG 2 cut(s) 931, 966
CviQI GTAC 8 cut(s) 22, 579, 770, 819, 929, 982, 1937, 2335
DdeI CTNAG 7 cut(s) 54, 882, 888, 1164, 1632, 2073, 2563
DraI TTTAAA 2 cut(s) 1075, 1524
DraIII CACNNNGTG 2 cut(s) 651, 1296
DrdI GACNNNNNNGTC 1 cut(s) 2416
DseDI GACNNNNNNGTC 1 cut(s) 2416
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 3 cut(s) 1419, 1683, 2427
EarI CTCTTC 3 cut(s) 1419, 1683, 2427
Ecl136II GAGCTC 2 cut(s) 58, 70
Eco130I CCWWGG 3 cut(s) 1332, 1614, 2685
Eco24I GRGCYC 2 cut(s) 60, 72
Eco31I GGTCTC 1 cut(s) 2424
Eco32I GATATC 2 cut(s) 513, 1262
Eco47I GGWCC 6 cut(s) 295, 1496, 2015, 2195, 2279, 2491
Eco53kI GAGCTC 2 cut(s) 58, 70
Eco57I CTGAAG 7 cut(s) 221, 368, 1023, 1443, 1512, 1800, 2186
Eco88I CYCGRG 2 cut(s) 2577, 2624
Eco91I GGTNACC 1 cut(s) 1952
EcoICRI GAGCTC 2 cut(s) 58, 70
EcoO109I RGGNCCY 2 cut(s) 295, 2279
EcoO65I GGTNACC 1 cut(s) 1952
EcoRII CCWGG 6 cut(s) 1056, 1965, 2221, 2455, 2496, 2544
EcoRV GATATC 2 cut(s) 513, 1262
EcoT14I CCWWGG 3 cut(s) 1332, 1614, 2685
EcoT38I GRGCYC 2 cut(s) 60, 72
ErhI CCWWGG 3 cut(s) 1332, 1614, 2685
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 2 cut(s) 1970, 2251
FauI CCCGC 2 cut(s) 2017, 2227
FauNDI CATATG 3 cut(s) 412, 434, 1879
FbaI TGATCA 2 cut(s) 1026, 1644
FokI GGATG 4 cut(s) 155, 413, 1631, 1834
FriOI GRGCYC 2 cut(s) 60, 72
FspBI CTAG 9 cut(s) 209, 254, 557, 582, 603, 816, 1863, 1890, 2141
GlaI GCGC 1 cut(s) 1126
GsaI CCCAGC 1 cut(s) 2042
GsuI CTGGAG 3 cut(s) 1208, 1719, 1917
HaeIII GGCC 5 cut(s) 443, 1229, 1571, 1763, 1965
HapII CCGG 4 cut(s) 328, 2426, 2625, 2657
HgaI GACGC 4 cut(s) 683, 710, 1574, 2031
HhaI GCGC 1 cut(s) 1127
Hin1I GRCGYC 1 cut(s) 1585
Hin6I GCGC 1 cut(s) 1125
HinP1I GCGC 1 cut(s) 1125
HincII GTYRAC 2 cut(s) 2046, 2079
HindII GTYRAC 2 cut(s) 2046, 2079
HindIII AAGCTT 1 cut(s) 1031
HpaII CCGG 4 cut(s) 328, 2426, 2625, 2657
HphI GGTGA 7 cut(s) 127, 280, 1370, 1812, 1964, 2479, 2596
Hpy166II GTNNAC 3 cut(s) 2046, 2060, 2079
Hpy188I TCNGA 8 cut(s) 225, 387, 420, 517, 833, 1042, 1112, 1816
Hpy8I GTNNAC 3 cut(s) 2046, 2060, 2079
HpyAV CCTTC 8 cut(s) 34, 106, 133, 392, 1487, 1753, 2084, 2210
HpyCH4IV ACGT 4 cut(s) 1709, 2258, 2361, 2550
HpyF3I CTNAG 7 cut(s) 54, 882, 888, 1164, 1632, 2073, 2563
HpySE526I ACGT 4 cut(s) 1709, 2258, 2361, 2550
Hsp92I GRCGYC 1 cut(s) 1585
HspAI GCGC 1 cut(s) 1125
Kpn2I TCCGGA 2 cut(s) 327, 2425
Ksp22I TGATCA 2 cut(s) 1026, 1644
LguI GCTCTTC 1 cut(s) 1419
LmnI GCTCC 7 cut(s) 67, 196, 627, 859, 1189, 1936, 2389
LweI GCATC 3 cut(s) 91, 1346, 1856
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 9 cut(s) 209, 254, 557, 582, 603, 816, 1863, 1890, 2141
MaeII ACGT 4 cut(s) 1709, 2258, 2361, 2550
MaeIII GTNAC 8 cut(s) 8, 605, 733, 865, 1649, 1747, 1952, 2254
MbiI CCGCTC 2 cut(s) 6, 1168
MfeI CAATTG 3 cut(s) 1442, 1448, 2213
MhlI GDGCHC 3 cut(s) 60, 72, 2074
MlsI TGGCCA 1 cut(s) 443
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 6 cut(s) 8, 1286, 2233, 2479, 2641, 2686
MmeI TCCRAC 2 cut(s) 820, 1047
Mox20I TGGCCA 1 cut(s) 443
MroI TCCGGA 2 cut(s) 327, 2425
MroXI GAANNNNTTC 1 cut(s) 1404
MscI TGGCCA 1 cut(s) 443
MseI TTAA 9 cut(s) 396, 635, 783, 963, 1074, 1197, 1479, 1523, 2340
MslI CAYNNNNRTG 2 cut(s) 437, 2612
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 5 cut(s) 779, 1163, 2012, 2189, 2234
MspCI CTTAAG 2 cut(s) 395, 1478
MspI CCGG 4 cut(s) 328, 2426, 2625, 2657
MspR9I CCNGG 9 cut(s) 1058, 1967, 2223, 2457, 2498, 2546, 2625, 2626, 2657
MunI CAATTG 3 cut(s) 1442, 1448, 2213
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 6 cut(s) 1058, 1967, 2223, 2457, 2498, 2546
MvnI CGCG 1 cut(s) 1125
NciI CCSGG 3 cut(s) 2625, 2626, 2657
NcoI CCATGG 1 cut(s) 2685
NdeI CATATG 3 cut(s) 412, 434, 1879
NlaIV GGNNCC 5 cut(s) 297, 855, 2136, 2197, 2208
NmuCI GTSAC 5 cut(s) 8, 605, 865, 1649, 1952
NspI RCATGY 5 cut(s) 181, 1439, 2085, 2515, 2540
OliI CACNNNNGTG 1 cut(s) 2612
PaeI GCATGC 1 cut(s) 181
PaeR7I CTCGAG 1 cut(s) 2577
PagI TCATGA 3 cut(s) 955, 1023, 2151
PaqCI CACCTGC 3 cut(s) 1831, 1927, 1933
PciI ACATGT 3 cut(s) 1435, 2081, 2511
PciSI GCTCTTC 1 cut(s) 1419
PctI GAATGC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 1404
PfeI GAWTC 6 cut(s) 287, 310, 494, 1655, 1817, 2155
PflMI CCANNNNNTGG 2 cut(s) 802, 2144
PleI GAGTC 6 cut(s) 8, 1285, 2233, 2479, 2641, 2686
PpsI GAGTC 6 cut(s) 8, 1285, 2233, 2479, 2641, 2686
PpuMI RGGWCCY 2 cut(s) 295, 2279
PscI ACATGT 3 cut(s) 1435, 2081, 2511
PsiI TTATAA 1 cut(s) 812
Psp124BI GAGCTC 2 cut(s) 60, 72
Psp5II RGGWCCY 2 cut(s) 295, 2279
Psp6I CCWGG 6 cut(s) 1056, 1965, 2221, 2455, 2496, 2544
PspEI GGTNACC 1 cut(s) 1952
PspFI CCCAGC 1 cut(s) 2038
PspGI CCWGG 6 cut(s) 1056, 1965, 2221, 2455, 2496, 2544
PspN4I GGNNCC 5 cut(s) 297, 855, 2136, 2197, 2208
PspPI GGNCC 8 cut(s) 295, 1496, 1569, 1963, 2015, 2195, 2279, 2491
PspPPI RGGWCCY 2 cut(s) 295, 2279
PspXI VCTCGAGB 1 cut(s) 2577
PstI CTGCAG 1 cut(s) 1888
PstNI CAGNNNCTG 1 cut(s) 2192
PvuII CAGCTG 3 cut(s) 779, 1163, 2189
RsaI GTAC 8 cut(s) 23, 580, 771, 820, 930, 983, 1938, 2336
RsaNI GTAC 8 cut(s) 22, 579, 770, 819, 929, 982, 1937, 2335
RseI CAYNNNNRTG 2 cut(s) 437, 2612
SacI GAGCTC 2 cut(s) 60, 72
SapI GCTCTTC 1 cut(s) 1419
SaqAI TTAA 9 cut(s) 396, 635, 783, 963, 1074, 1197, 1479, 1523, 2340
Sau96I GGNCC 8 cut(s) 295, 1496, 1569, 1963, 2015, 2195, 2279, 2491
ScaI AGTACT 3 cut(s) 23, 580, 820
SchI GAGTC 6 cut(s) 8, 1286, 2233, 2479, 2641, 2686
ScrFI CCNGG 9 cut(s) 1058, 1967, 2223, 2457, 2498, 2546, 2625, 2626, 2657
SduI GDGCHC 3 cut(s) 60, 72, 2074
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 3 cut(s) 91, 1346, 1856
SfcI CTRYAG 1 cut(s) 1884
Sfr274I CTCGAG 1 cut(s) 2577
SinI GGWCC 6 cut(s) 295, 1496, 2015, 2195, 2279, 2491
SlaI CTCGAG 1 cut(s) 2577
SmaI CCCGGG 1 cut(s) 2626
SmiMI CAYNNNNRTG 2 cut(s) 437, 2612
SmlI CTYRAG 7 cut(s) 395, 500, 1478, 2274, 2405, 2577, 2699
SmoI CTYRAG 7 cut(s) 395, 500, 1478, 2274, 2405, 2577, 2699
SpeI ACTAGT 3 cut(s) 602, 815, 1862
SphI GCATGC 1 cut(s) 181
SsiI CCGC 9 cut(s) 6, 266, 764, 1123, 1168, 1857, 2010, 2234, 2494
SspI AATATT 1 cut(s) 2100
SspMI CTAG 9 cut(s) 209, 254, 557, 582, 603, 816, 1863, 1890, 2141
SstI GAGCTC 2 cut(s) 60, 72
StyD4I CCNGG 9 cut(s) 1056, 1965, 2221, 2455, 2496, 2544, 2623, 2624, 2655
StyI CCWWGG 3 cut(s) 1332, 1614, 2685
TaiI ACGT 4 cut(s) 1712, 2261, 2364, 2553
TaqI TCGA 2 cut(s) 2118, 2578
TaqII GACCGA 1 cut(s) 1059
TatI WGTACW 6 cut(s) 21, 578, 769, 818, 928, 981
TfiI GAWTC 6 cut(s) 287, 310, 494, 1655, 1817, 2155
Tru1I TTAA 9 cut(s) 396, 635, 783, 963, 1074, 1197, 1479, 1523, 2340
Tru9I TTAA 9 cut(s) 396, 635, 783, 963, 1074, 1197, 1479, 1523, 2340
TscAI CASTG 6 cut(s) 876, 1647, 1654, 1981, 2074, 2614
TseFI GTSAC 5 cut(s) 8, 605, 865, 1649, 1952
Tsp45I GTSAC 5 cut(s) 8, 605, 865, 1649, 1952
TspDTI ATGAA 7 cut(s) 138, 654, 655, 972, 1335, 2140, 2509
TspGWI ACGGA 2 cut(s) 1103, 2010
TspMI CCCGGG 1 cut(s) 2624
TspRI CASTG 6 cut(s) 876, 1647, 1654, 1981, 2074, 2614
Van91I CCANNNNNTGG 2 cut(s) 802, 2144
Vha464I CTTAAG 2 cut(s) 395, 1478
VpaK11BI GGWCC 6 cut(s) 295, 1496, 2015, 2195, 2279, 2491
XapI RAATTY 2 cut(s) 564, 1810
XceI RCATGY 5 cut(s) 181, 1439, 2085, 2515, 2540
XhoI CTCGAG 1 cut(s) 2577
XmaI CCCGGG 1 cut(s) 2624
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 9 cut(s) 209, 254, 557, 582, 603, 816, 1863, 1890, 2141
ZrmI AGTACT 3 cut(s) 23, 580, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.