MD06G1087800.v1.1

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006717
Physical Location & Seq
Reverse (-)
21455764 .. 21462623
6860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1087800.v1.1.491

Sequence Viewer

Length: 2712 bp
ATGAGCGGGCACGACTCCAAGTACTTCTCCACCACCAAGAAGGGCGAAATCCCTGAGCTCAAAGAGGAGCTCAATTCTCAATACAAGGATAAGAGAAAAGATGCGGTCAAGAAGGTGATCGCCGCAATGACTGTGGGGAAGGATGTTTCGTCACTTTTCACAGATGTAGTGAATTGCATGCAAACAGAAAACTTGGAGCTGAAGAAGCTTGTCTATTTGTATCTGATAAATTATGCTAAAAGCCAGCCTGACCTAGCGATACTTGCAGTGAATACATTTGTCAAGGATTCACAGGACCCAAACCCTTTGATTCGTGCTTTAGCCGTCCGGACAATGGGTTGCATTCGTGTTGATAAAATTACAGAATATCTATGTGATCCCCTTCAGAGATGTCTCAAGGATGATGATCCTTATGTGCGGAAGACAGCAGCCATATGTGTGGCCAAACTTTATGACATAAATGCAGAGTTAGTTGAGGATAGGGGTTTTCTGGAATCCCTCAAGGATTTGATATCTGATAATAATCCTATGGTTGTAGCAAATGCTGTGGCTGCTCTAGCCGAAATTCAAGAGAATAGTAATAGACCCATCTTTGAGATCACTAGTCACACTCTGTCAAAGCTCCTTACCGCCTTAAATGAATGTACAGAGTGGGGTCAAGTTTTCATATTGGACGCTCTTTCTAAATACAAGGCAGCAGACGCTCGTGAAGCTGAAAATATAGTAGAGCGAGTTACACCACGATTACAACATGCTAACTGTGCGGTTGTACTTTCAGCTGTTAAGATGATCCTCCAACAGATGGAACTTATAACTAGTACTGATGTCGTCAGAAATCTTTGCAAAAAGATGGCTCCTCCACTCGTGACACTACTATCTGCTGAGCCTGAGATACAATATGTTGCATTGAGAAACATCAACCTTATAGTGCAGAGACGACCCACAATCCTCGCTCATGAAATTAAGGTGTTCTTCTGCAAGTACAATGATCCAATTTATGTAAAGATGGAAAAGTTAGAAATCATGATAAAGCTTGCTTCAGACCGAAATATAGACCAGGTTTTGTTGGAGTTTAAAGAGTATGCTACCGAAGTAGATGTGGATTTTGTCCGAAAAGCTGTCCGTGCCATTGGTCGCTGTGCCATCAAATTAGAAAGAGCAGCTGAGCGGTGCATTACTGTTTTACTGGAGCTTATTAAGATCAAAGTAAATTATGTAGTTCAAGAGGCCATTATTGTCATCAAAGATATATTTAGAAGATATCCAAATACCTACGAGTCCATTATTGCAACTCTGTGCGAAAGCCTTGACACTTTAGATGAACCAGAAGCCAAGGCATCCATGATCTGGATAATTGGTGAATATGCGGAACGAATTGACAATGCTGATGAGCTACTTGAAAGTTTTCTGGAAAGTTTCCCTGAAGAGCCTGCACATGTCCAATTGCAATTGCTGACAGCAACTGTTAAACTCTTTCTTAAGAAGCCAACTGAAGGACCACAGCAGATGATACAGGTTGTTTTAAATAATGCTACTGTGGAAACAGACAATCCTGATTTGCGAGATCGGGCCTACATATATTGGCGTCTTCTATCAACTGACCCAGAGGCAGCCAAGGATGTGGTGTTAGCTGAGAAGCCAGTGATCAGTGATGATTCAAACCTGATTGATCCATCTCTTCTTGATGAGCTTCTTGCTAACATTGCTACGTTATCCTCTGTGTATCACAAGCCTCCAGAAGCATTTGTTACCCGTGTGAAGACCACAGCCCAAAGAACTGAAGATGAGGACTATGGTAGTGAAACAGGAAATTCTGAATCACCTGCTCATGTTGCTGATAGTTCTGCATCCCCAACCGGCTCTAGTTCAGGTGTTCCATATGCTGCATCTAGACAACCTGCACCAGTTTCGCCTGCACCTGCACCTGCACCTGCTCCAGTGCCAGATTTACTTGGTGACCTGATCGGCCTGGAAAATAGTGCTATTGTCCCTGTGGATCAGCCTGCTTCTCCTGCTGGACCTCCGTTGCCTGTTGTGCTACCAGCATCTACTGGTCAAGGTTTACAAATCAGTGCTCAGTTGACACGTAGGGAAGGGCAAATATTTTACAGTTTATTGTTCGAGAACAACACACAGGTTCCGCTGGATGGGTTCATGATTCAGTTCAACAAAAATACATTTGGGCTTGCAGCGGCTGGACCCCTTCAGGTTCCACAATTGCAACCTGGGACATCTGCCGGGACTCTCTTGCCTATGGTTACGTTCCAGAATATGTCTCAAGGTCCTCCAAGCTCACTATTGCAGGTTGCTGTGAAAAACAGTCAACAACCAGTCTGGTACTTTAATGATAAAATATCATTGCATGTCTTCTTTACTGATGATGGAAGAATGGAGCGTGCAAATTTTCTTGAGACGTGGAGGTCTCTTCCGGATACAAATGAGATTACAAAGGACTTCCCTGGAATTGTGTTGAGAAATGTTGAAGCGACTGTGGACCGCCTGGCTGCTACAAACATGTTCTTTATTGCAAAGCGCAAACATGCCAACCAGGACGTGTTCTATTTCTCTGCAAAAATCCCTCGAGGAATACCTTTCTTGATTGAACTCACCACAGTCGTGAACAATCCCGGGGTCAAGATTGCGATCAAGACTCCAAGCCCGGAGACGGCACCTCTTTTCTTTGAAGCCATGGAGACTCTCCTCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

904

Amino Acids

100.34

Weight (kDa)

5.1

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 14 - 534 1.2e-169 Adaptin N terminal region
HEAT_2 PF13646 98 - 187 8.8e-09 HEAT repeats
Cnd1 PF12717 103 - 266 3.4e-66 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 677 - 781 2.5e-11 Adaptin C-terminal domain
B2-adapt-app_C PF09066 793 - 901 1.7e-30 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
AarI CACCTGC 4 cut(s) 1831, 1927, 1933, 1939
AasI GACNNNNNNGTC 1 cut(s) 2419
AbsI CCTCGAGG 1 cut(s) 2580
Acc36I ACCTGC 6 cut(s) 1831, 1906, 1927, 1933, 1939, 2293
AccB1I GGYRCC 1 cut(s) 2668
AccB7I CCANNNNNTGG 2 cut(s) 802, 1347
AccBSI CCGCTC 2 cut(s) 6, 1168
AccIII TCCGGA 2 cut(s) 327, 2428
AclWI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2004
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 3 cut(s) 564, 1810, 2401
AcuI CTGAAG 7 cut(s) 221, 368, 1023, 1443, 1512, 1800, 2189
AcyI GRCGYC 1 cut(s) 1585
AfaI GTAC 6 cut(s) 23, 646, 771, 820, 983, 2339
AfiI CCNNNNNNNGG 6 cut(s) 334, 802, 1347, 1493, 2147, 2665
AflII CTTAAG 1 cut(s) 1478
AflIII ACRYGT 4 cut(s) 1435, 2084, 2514, 2553
AgsI TTSAA 8 cut(s) 569, 1223, 1400, 1659, 2167, 2483, 2603, 2684
AhlI ACTAGT 2 cut(s) 602, 815
AjiI CACGTC 2 cut(s) 2415, 2554
AjnI CCWGG 6 cut(s) 1056, 1968, 2224, 2458, 2499, 2547
AleI CACNNNNGTG 1 cut(s) 2615
Alw21I GWGCWC 3 cut(s) 60, 72, 2077
Alw26I GTCTC 7 cut(s) 398, 928, 2280, 2405, 2427, 2657, 2687
AlwI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2004
AlwNI CAGNNNCTG 2 cut(s) 1463, 2195
Ama87I CYCGRG 2 cut(s) 2580, 2627
Aor13HI TCCGGA 2 cut(s) 327, 2428
AoxI GGCC 4 cut(s) 441, 1227, 1569, 1966
ApeKI GCWGC 8 cut(s) 428, 551, 695, 1160, 1610, 1883, 2189, 2504
ApoI RAATTY 3 cut(s) 564, 1810, 2401
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 1 cut(s) 2535
AspS9I GGNCC 7 cut(s) 295, 1496, 1569, 2018, 2198, 2282, 2494
AsuC2I CCSGG 4 cut(s) 2239, 2628, 2629, 2660
AsuHPI GGTGA 5 cut(s) 127, 1370, 1812, 1967, 2599
AvaI CYCGRG 2 cut(s) 2580, 2627
AvaII GGWCC 6 cut(s) 295, 1496, 2018, 2198, 2282, 2494
BaeGI GKGCMC 1 cut(s) 12
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 1 cut(s) 2668
BanII GRGCYC 2 cut(s) 60, 72
BauI CACGAG 2 cut(s) 705, 863
BbsI GAAGAC 4 cut(s) 428, 1580, 1766, 2359
Bbv12I GWGCWC 3 cut(s) 60, 72, 2077
BbvI GCAGC 8 cut(s) 440, 538, 707, 1172, 1622, 1870, 2201, 2491
BccI CCATC 8 cut(s) 596, 796, 844, 1000, 1151, 1681, 2141, 2375
BceAI ACGGC 2 cut(s) 308, 2682
BcgI CGANNNNNNTGC 2 cut(s) 1890, 1924
BciT130I CCWGG 6 cut(s) 1058, 1970, 2226, 2460, 2501, 2549
BciVI GTATCC 1 cut(s) 2425
BclI TGATCA 1 cut(s) 1644
BcnI CCSGG 4 cut(s) 2239, 2628, 2629, 2660
BcoDI GTCTC 7 cut(s) 398, 928, 2280, 2405, 2427, 2657, 2687
BcuI ACTAGT 2 cut(s) 602, 815
BfaI CTAG 6 cut(s) 254, 557, 603, 816, 1863, 1890
BfrI CTTAAG 1 cut(s) 1478
BfuAI ACCTGC 6 cut(s) 1831, 1906, 1927, 1933, 1939, 2293
BfuI GTATCC 1 cut(s) 2425
BlpI GCTNAGC 2 cut(s) 882, 1164
BmcAI AGTACT 2 cut(s) 23, 820
Bme18I GGWCC 6 cut(s) 295, 1496, 2018, 2198, 2282, 2494
BmeT110I CYCGRG 2 cut(s) 2580, 2627
BmgBI CACGTC 2 cut(s) 2415, 2554
BmgT120I GGNCC 7 cut(s) 295, 1496, 1569, 2018, 2198, 2282, 2494
BmiI GGNNCC 6 cut(s) 297, 855, 2139, 2200, 2211, 2670
BmsI GCATC 5 cut(s) 91, 1346, 1856, 1895, 2054
BpiI GAAGAC 4 cut(s) 428, 1580, 1766, 2359
BpmI CTGGAG 3 cut(s) 1208, 1719, 1920
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 2 cut(s) 882, 1164
BpuEI CTTGAG 5 cut(s) 380, 485, 2262, 2429, 2687
BpuMI CCSGG 4 cut(s) 2239, 2628, 2629, 2660
BsaAI YACGTR 1 cut(s) 2087
BsaBI GATNNNNATC 3 cut(s) 405, 522, 2642
BsaHI GRCGYC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 2427
BsaJI CCNNGG 7 cut(s) 1332, 1614, 2225, 2458, 2627, 2628, 2688
BsaWI WCCGGW 2 cut(s) 327, 2428
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 802, 1347, 1493, 2147, 2665
Bse118I RCCGGY 1 cut(s) 1856
Bse1I ACTGG 6 cut(s) 1191, 1640, 1904, 1937, 2056, 2330
Bse3DI GCAATG 3 cut(s) 132, 1701, 2357
Bse8I GATNNNNATC 3 cut(s) 405, 522, 2642
BseAI TCCGGA 2 cut(s) 327, 2428
BseBI CCWGG 6 cut(s) 1058, 1970, 2226, 2460, 2501, 2549
BseDI CCNNGG 7 cut(s) 1332, 1614, 2225, 2458, 2627, 2628, 2688
BseGI GGATG 6 cut(s) 148, 406, 1337, 1624, 1847, 2152
BseJI GATNNNNATC 3 cut(s) 405, 522, 2642
BseLI CCNNNNNNNGG 6 cut(s) 334, 802, 1347, 1493, 2147, 2665
BseMI GCAATG 3 cut(s) 132, 1701, 2357
BseMII CTCAG 6 cut(s) 45, 873, 879, 1155, 1623, 2090
BseNI ACTGG 6 cut(s) 1191, 1640, 1904, 1937, 2056, 2330
BseRI GAGGAG 3 cut(s) 80, 846, 2690
BseSI GKGCMC 1 cut(s) 12
BseXI GCAGC 8 cut(s) 440, 538, 707, 1172, 1622, 1870, 2201, 2491
BsgI GTGCAG 6 cut(s) 950, 1416, 1884, 1899, 1905, 1911
BshFI GGCC 4 cut(s) 443, 1229, 1571, 1968
BshNI GGYRCC 1 cut(s) 2668
BsiHKAI GWGCWC 3 cut(s) 60, 72, 2077
BsiHKCI CYCGRG 2 cut(s) 2580, 2627
BsiSI CCGG 6 cut(s) 328, 1857, 2238, 2429, 2628, 2660
BslFI GGGAC 3 cut(s) 1973, 2242, 2254
BslI CCNNNNNNNGG 6 cut(s) 334, 802, 1347, 1493, 2147, 2665
BsmAI GTCTC 7 cut(s) 398, 928, 2280, 2405, 2427, 2657, 2687
BsmBI CGTCTC 3 cut(s) 928, 2405, 2657
BsmFI GGGAC 3 cut(s) 1973, 2242, 2254
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 4 cut(s) 443, 1229, 1571, 1968
Bso31I GGTCTC 1 cut(s) 2427
BsoBI CYCGRG 2 cut(s) 2580, 2627
Bsp1286I GDGCHC 4 cut(s) 12, 60, 72, 2077
Bsp13I TCCGGA 2 cut(s) 327, 2428
Bsp1407I TGTACA 1 cut(s) 644
Bsp1720I GCTNAGC 2 cut(s) 882, 1164
Bsp19I CCATGG 1 cut(s) 2688
BspANI GGCC 4 cut(s) 443, 1229, 1571, 1968
BspCNI CTCAG 6 cut(s) 46, 874, 880, 1156, 1624, 2089
BspEI TCCGGA 2 cut(s) 327, 2428
BspHI TCATGA 3 cut(s) 955, 1023, 2154
BspLI GGNNCC 6 cut(s) 297, 855, 2139, 2200, 2211, 2670
BspMI ACCTGC 6 cut(s) 1831, 1906, 1927, 1933, 1939, 2293
BspPI GGATC 6 cut(s) 371, 401, 784, 983, 1664, 2004
BspQI GCTCTTC 1 cut(s) 1419
BspT107I GGYRCC 1 cut(s) 2668
BspTI CTTAAG 1 cut(s) 1478
BspTNI GGTCTC 1 cut(s) 2427
BsrBI CCGCTC 2 cut(s) 6, 1168
BsrDI GCAATG 3 cut(s) 132, 1701, 2357
BsrFI RCCGGY 1 cut(s) 1856
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 6 cut(s) 1191, 1640, 1904, 1937, 2056, 2330
BssAI RCCGGY 1 cut(s) 1856
BssECI CCNNGG 7 cut(s) 1332, 1614, 2225, 2458, 2627, 2628, 2688
BssNI GRCGYC 1 cut(s) 1585
BssSI CACGAG 2 cut(s) 705, 863
BssT1I CCWWGG 3 cut(s) 1332, 1614, 2688
Bst2BI CACGAG 2 cut(s) 705, 863
Bst2UI CCWGG 6 cut(s) 1058, 1970, 2226, 2460, 2501, 2549
Bst4CI ACNGT 9 cut(s) 133, 761, 1180, 1465, 1537, 2111, 2321, 2491, 2614
Bst6I CTCTTC 3 cut(s) 1419, 1683, 2430
BstACI GRCGYC 1 cut(s) 1585
BstAFI CTTAAG 1 cut(s) 1478
BstAUI TGTACA 1 cut(s) 644
BstBAI YACGTR 1 cut(s) 2087
BstC8I GCNNGC 9 cut(s) 8, 179, 245, 1035, 1431, 1914, 2004, 2187, 2397
BstDEI CTNAG 6 cut(s) 54, 882, 888, 1164, 1632, 2076
BstDSI CCRYGG 1 cut(s) 2688
BstEII GGTNACC 1 cut(s) 1955
BstF5I GGATG 6 cut(s) 148, 406, 1337, 1624, 1847, 2152
BstHHI GCGC 1 cut(s) 2535
BstMAI GTCTC 7 cut(s) 398, 928, 2280, 2405, 2427, 2657, 2687
BstNI CCWGG 6 cut(s) 1058, 1970, 2226, 2460, 2501, 2549
BstNSI RCATGY 6 cut(s) 181, 755, 1439, 2366, 2518, 2543
BstPI GGTNACC 1 cut(s) 1955
BstSLI GKGCMC 1 cut(s) 12
BstV1I GCAGC 8 cut(s) 440, 538, 707, 1172, 1622, 1870, 2201, 2491
BstV2I GAAGAC 4 cut(s) 428, 1580, 1766, 2359
BstXI CCANNNNNNTGG 2 cut(s) 439, 1621
BsuI GTATCC 1 cut(s) 2425
BsuRI GGCC 4 cut(s) 443, 1229, 1571, 1968
BtgI CCRYGG 1 cut(s) 2688
BtrI CACGTC 2 cut(s) 2415, 2554
BtsCI GGATG 6 cut(s) 148, 406, 1337, 1624, 1847, 2152
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 5 cut(s) 273, 1647, 1654, 1944, 2077
BveI ACCTGC 6 cut(s) 1831, 1906, 1927, 1933, 1939, 2293
Cac8I GCNNGC 9 cut(s) 8, 179, 245, 1035, 1431, 1914, 2004, 2187, 2397
CaiI CAGNNNCTG 2 cut(s) 1463, 2195
CciI TCATGA 3 cut(s) 955, 1023, 2154
CfoI GCGC 1 cut(s) 2535
Cfr10I RCCGGY 1 cut(s) 1856
Cfr13I GGNCC 7 cut(s) 295, 1496, 1569, 2018, 2198, 2282, 2494
Cfr9I CCCGGG 1 cut(s) 2627
CseI GACGC 3 cut(s) 683, 710, 1574
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 6 cut(s) 22, 645, 770, 819, 982, 2338
CspCI CAANNNNNGTGG 4 cut(s) 114, 149, 528, 563
CviQI GTAC 6 cut(s) 22, 645, 770, 819, 982, 2338
DdeI CTNAG 6 cut(s) 54, 882, 888, 1164, 1632, 2076
DraI TTTAAA 2 cut(s) 1075, 1524
DrdI GACNNNNNNGTC 1 cut(s) 2419
DseDI GACNNNNNNGTC 1 cut(s) 2419
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 3 cut(s) 1419, 1683, 2430
EarI CTCTTC 3 cut(s) 1419, 1683, 2430
Ecl136II GAGCTC 2 cut(s) 58, 70
Eco130I CCWWGG 3 cut(s) 1332, 1614, 2688
Eco24I GRGCYC 2 cut(s) 60, 72
Eco31I GGTCTC 1 cut(s) 2427
Eco32I GATATC 2 cut(s) 513, 1262
Eco47I GGWCC 6 cut(s) 295, 1496, 2018, 2198, 2282, 2494
Eco53kI GAGCTC 2 cut(s) 58, 70
Eco57I CTGAAG 7 cut(s) 221, 368, 1023, 1443, 1512, 1800, 2189
Eco88I CYCGRG 2 cut(s) 2580, 2627
Eco91I GGTNACC 1 cut(s) 1955
EcoICRI GAGCTC 2 cut(s) 58, 70
EcoO109I RGGNCCY 2 cut(s) 295, 2282
EcoO65I GGTNACC 1 cut(s) 1955
EcoRII CCWGG 6 cut(s) 1056, 1968, 2224, 2458, 2499, 2547
EcoRV GATATC 2 cut(s) 513, 1262
EcoT14I CCWWGG 3 cut(s) 1332, 1614, 2688
EcoT38I GRGCYC 2 cut(s) 60, 72
ErhI CCWWGG 3 cut(s) 1332, 1614, 2688
Esp3I CGTCTC 3 cut(s) 928, 2405, 2657
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 3 cut(s) 1973, 2242, 2254
FauNDI CATATG 2 cut(s) 434, 1879
FbaI TGATCA 1 cut(s) 1644
FokI GGATG 6 cut(s) 155, 413, 1324, 1631, 1834, 2159
FriOI GRGCYC 2 cut(s) 60, 72
FspBI CTAG 6 cut(s) 254, 557, 603, 816, 1863, 1890
GlaI GCGC 1 cut(s) 2534
GsuI CTGGAG 3 cut(s) 1208, 1719, 1920
HaeIII GGCC 4 cut(s) 443, 1229, 1571, 1968
HapII CCGG 6 cut(s) 328, 1857, 2238, 2429, 2628, 2660
HgaI GACGC 3 cut(s) 683, 710, 1574
HhaI GCGC 1 cut(s) 2535
Hin1I GRCGYC 1 cut(s) 1585
Hin6I GCGC 1 cut(s) 2533
HinP1I GCGC 1 cut(s) 2533
HincII GTYRAC 2 cut(s) 2082, 2324
HindII GTYRAC 2 cut(s) 2082, 2324
HindIII AAGCTT 2 cut(s) 206, 1031
HpaII CCGG 6 cut(s) 328, 1857, 2238, 2429, 2628, 2660
HphI GGTGA 5 cut(s) 127, 1370, 1812, 1967, 2599
Hpy166II GTNNAC 5 cut(s) 2063, 2082, 2324, 2494, 2620
Hpy188I TCNGA 7 cut(s) 225, 387, 517, 833, 1042, 1112, 1816
Hpy8I GTNNAC 5 cut(s) 2063, 2082, 2324, 2494, 2620
HpyAV CCTTC 7 cut(s) 34, 106, 133, 392, 1487, 2087, 2213
HpyCH4III ACNGT 9 cut(s) 133, 761, 1180, 1465, 1537, 2111, 2321, 2491, 2614
HpyCH4IV ACGT 5 cut(s) 1709, 2086, 2261, 2414, 2553
HpyF3I CTNAG 6 cut(s) 54, 882, 888, 1164, 1632, 2076
HpySE526I ACGT 5 cut(s) 1709, 2086, 2261, 2414, 2553
Hsp92I GRCGYC 1 cut(s) 1585
HspAI GCGC 1 cut(s) 2533
Kpn2I TCCGGA 2 cut(s) 327, 2428
Ksp22I TGATCA 1 cut(s) 1644
LguI GCTCTTC 1 cut(s) 1419
LmnI GCTCC 7 cut(s) 67, 196, 627, 859, 1189, 1939, 2392
Lsp1109I GCAGC 8 cut(s) 440, 538, 707, 1172, 1622, 1870, 2201, 2491
LweI GCATC 5 cut(s) 91, 1346, 1856, 1895, 2054
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 6 cut(s) 254, 557, 603, 816, 1863, 1890
MaeII ACGT 5 cut(s) 1709, 2086, 2261, 2414, 2553
MaeIII GTNAC 7 cut(s) 150, 605, 733, 865, 1747, 1955, 2257
MbiI CCGCTC 2 cut(s) 6, 1168
MfeI CAATTG 3 cut(s) 1442, 1448, 2216
MhlI GDGCHC 4 cut(s) 12, 60, 72, 2077
MlsI TGGCCA 1 cut(s) 443
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 5 cut(s) 8, 1286, 2236, 2644, 2689
MmeI TCCRAC 2 cut(s) 820, 1047
Mox20I TGGCCA 1 cut(s) 443
MroI TCCGGA 2 cut(s) 327, 2428
MroXI GAANNNNTTC 1 cut(s) 1404
MscI TGGCCA 1 cut(s) 443
MseI TTAA 9 cut(s) 635, 783, 963, 1074, 1197, 1467, 1479, 1523, 2343
MslI CAYNNNNRTG 2 cut(s) 437, 2615
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 4 cut(s) 779, 1163, 2143, 2192
MspCI CTTAAG 1 cut(s) 1478
MspI CCGG 6 cut(s) 328, 1857, 2238, 2429, 2628, 2660
MunI CAATTG 3 cut(s) 1442, 1448, 2216
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 6 cut(s) 1058, 1970, 2226, 2460, 2501, 2549
NciI CCSGG 4 cut(s) 2239, 2628, 2629, 2660
NcoI CCATGG 1 cut(s) 2688
NdeI CATATG 2 cut(s) 434, 1879
NlaIV GGNNCC 6 cut(s) 297, 855, 2139, 2200, 2211, 2670
NmuCI GTSAC 4 cut(s) 150, 605, 865, 1955
NspI RCATGY 6 cut(s) 181, 755, 1439, 2366, 2518, 2543
OliI CACNNNNGTG 1 cut(s) 2615
PaeI GCATGC 1 cut(s) 181
PaeR7I CTCGAG 1 cut(s) 2580
PagI TCATGA 3 cut(s) 955, 1023, 2154
PaqCI CACCTGC 4 cut(s) 1831, 1927, 1933, 1939
PciI ACATGT 2 cut(s) 1435, 2514
PciSI GCTCTTC 1 cut(s) 1419
PctI GAATGC 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 1404
PfeI GAWTC 6 cut(s) 287, 310, 494, 1655, 1817, 2158
PflMI CCANNNNNTGG 2 cut(s) 802, 1347
PleI GAGTC 5 cut(s) 8, 1285, 2236, 2644, 2689
PpsI GAGTC 5 cut(s) 8, 1285, 2236, 2644, 2689
Ppu21I YACGTR 1 cut(s) 2087
PpuMI RGGWCCY 2 cut(s) 295, 2282
PscI ACATGT 2 cut(s) 1435, 2514
PsiI TTATAA 1 cut(s) 812
Psp124BI GAGCTC 2 cut(s) 60, 72
Psp5II RGGWCCY 2 cut(s) 295, 2282
Psp6I CCWGG 6 cut(s) 1056, 1968, 2224, 2458, 2499, 2547
PspEI GGTNACC 1 cut(s) 1955
PspGI CCWGG 6 cut(s) 1056, 1968, 2224, 2458, 2499, 2547
PspN4I GGNNCC 6 cut(s) 297, 855, 2139, 2200, 2211, 2670
PspPI GGNCC 7 cut(s) 295, 1496, 1569, 2018, 2198, 2282, 2494
PspPPI RGGWCCY 2 cut(s) 295, 2282
PspXI VCTCGAGB 1 cut(s) 2580
PstNI CAGNNNCTG 2 cut(s) 1463, 2195
PvuII CAGCTG 2 cut(s) 779, 1163
RsaI GTAC 6 cut(s) 23, 646, 771, 820, 983, 2339
RsaNI GTAC 6 cut(s) 22, 645, 770, 819, 982, 2338
RseI CAYNNNNRTG 2 cut(s) 437, 2615
SacI GAGCTC 2 cut(s) 60, 72
SapI GCTCTTC 1 cut(s) 1419
SaqAI TTAA 9 cut(s) 635, 783, 963, 1074, 1197, 1467, 1479, 1523, 2343
Sau96I GGNCC 7 cut(s) 295, 1496, 1569, 2018, 2198, 2282, 2494
ScaI AGTACT 2 cut(s) 23, 820
SchI GAGTC 5 cut(s) 8, 1286, 2236, 2644, 2689
SduI GDGCHC 4 cut(s) 12, 60, 72, 2077
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 5 cut(s) 91, 1346, 1856, 1895, 2054
Sfr274I CTCGAG 1 cut(s) 2580
SinI GGWCC 6 cut(s) 295, 1496, 2018, 2198, 2282, 2494
SlaI CTCGAG 1 cut(s) 2580
SmaI CCCGGG 1 cut(s) 2629
SmiMI CAYNNNNRTG 2 cut(s) 437, 2615
SmlI CTYRAG 7 cut(s) 395, 500, 1478, 2277, 2408, 2580, 2702
SmoI CTYRAG 7 cut(s) 395, 500, 1478, 2277, 2408, 2580, 2702
SpeI ACTAGT 2 cut(s) 602, 815
SphI GCATGC 1 cut(s) 181
SspI AATATT 1 cut(s) 2103
SspMI CTAG 6 cut(s) 254, 557, 603, 816, 1863, 1890
SstI GAGCTC 2 cut(s) 60, 72
StyI CCWWGG 3 cut(s) 1332, 1614, 2688
TaaI ACNGT 9 cut(s) 133, 761, 1180, 1465, 1537, 2111, 2321, 2491, 2614
TaiI ACGT 5 cut(s) 1712, 2089, 2264, 2417, 2556
TaqI TCGA 2 cut(s) 2121, 2581
TaqII GACCGA 1 cut(s) 1059
TatI WGTACW 5 cut(s) 21, 644, 769, 818, 981
TauI GCSGC 2 cut(s) 125, 2195
TfiI GAWTC 6 cut(s) 287, 310, 494, 1655, 1817, 2158
Tru1I TTAA 9 cut(s) 635, 783, 963, 1074, 1197, 1467, 1479, 1523, 2343
Tru9I TTAA 9 cut(s) 635, 783, 963, 1074, 1197, 1467, 1479, 1523, 2343
TscAI CASTG 5 cut(s) 273, 1647, 1654, 1944, 2077
TseFI GTSAC 4 cut(s) 150, 605, 865, 1955
TseI GCWGC 8 cut(s) 428, 551, 695, 1160, 1610, 1883, 2189, 2504
Tsp45I GTSAC 4 cut(s) 150, 605, 865, 1955
TspDTI ATGAA 5 cut(s) 654, 655, 972, 1335, 2143
TspGWI ACGGA 2 cut(s) 1112, 2013
TspMI CCCGGG 1 cut(s) 2627
TspRI CASTG 5 cut(s) 273, 1647, 1654, 1944, 2077
Van91I CCANNNNNTGG 2 cut(s) 802, 1347
Vha464I CTTAAG 1 cut(s) 1478
VpaK11BI GGWCC 6 cut(s) 295, 1496, 2018, 2198, 2282, 2494
XapI RAATTY 3 cut(s) 564, 1810, 2401
XbaI TCTAGA 1 cut(s) 1889
XceI RCATGY 6 cut(s) 181, 755, 1439, 2366, 2518, 2543
XhoI CTCGAG 1 cut(s) 2580
XmaI CCCGGG 1 cut(s) 2627
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 6 cut(s) 254, 557, 603, 816, 1863, 1890
ZrmI AGTACT 2 cut(s) 23, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.