RLG00000003961

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006717
Physical Location & Seq
Reverse (-)
54740395 .. 54751729
11335 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003961

Sequence Viewer

Length: 3354 bp
ATGAGCGGGCACGACTCCAAGTACTTCTCCACCACCAAGAAGGGTGAAATTCCCGAGCTCAAAGAAGAGCTCAATTCTCAATACAAGGATAAGAGGAAAGATGCTGTTAAGAAGGTGATTGCTGCAATGACAGTTGGGAAGGATGTCTCATCTCTTTTCACGGATGTAGTGAATTGCATGCAAACAGAAAATTTGGAGCTGAAGAAGCTGGTTTATTTGTATCTGATAAATTATGCTAAAAGCCAGCCTGACCTAGCAATTCTTGCTGTGAATACATTTGTCAAGGATTCACAGGACCCAAATCCTTTGATTCGTGCGTTAGCCGTTCGGACAATGGGATGCATTCGTGTTGATAAAATTACAGAATATCTGTGTGATCCCCTTCAGAGATGCCTTAAGGATGATGATCCATATGTTCGCAAGACAGCAGCCATATGTGTCGCCAAACTTTACGACATAAATGCTGAGTTAGTTGAGGATAGGGGTTTCCTGGAATCTCTGAAGGATTTGATTTCTGATAATAATCCTATGGTTGTTGCAAATGCTGTGGCTGCGCTTGCTGAAATTCAAGAGAATAGTAATAGACCCATCTTTGAAATGACCAGTCACACTCTGACAAAGCTCCTTACTGCCTTGAATGAATGCACAGAGTGGGGACAAGTATTTATACTGGATGCTCTTTCTAGATACAAGGCAGCTGATGCCCGTGAAGCTGAAAATATAGTAGAGCGAGTTACCCCGCGACTACAGCATGCTAACTGTGCTGTCGTGCTTTCAGCTGTAAAGATGATCCTCCAACAAATGGAACTTATCACCAGTACCGATGTTGTCAGAAATCTTTGCAAAAAGATGGCTCCTCCACTCGTGACACTACTCTCTGCTGAGCCTGAGATACAATATGTGGCATTGAGAAACATCAACCTTATAGTTCAAAGACGGCCCACAATTCTTGCTCATGAAATTAAGGTATTCTTTTGCAAGTACAATGATCCTATTTATGTAAAGATGGAAAAGTTAGAAATTATGATAAAGCTTGCTTCAGATCGAAACATAGACCAGGTTCTATTGGAGTTTAAAGAGTATGCTACTGAAGTAGATGTTGATTTTGTCCGAAAGGCTGTCCGTGCCATTGGTCGCTGTGCCATCAAATTAGAAAGAGCAGCTGAACGGTGCATTACTGTATTACTTGAGTTGATTAAGATCAAAGTCAATTACGTAGTTCAAGAGGCCATTATTGTCATCAAAGATATCTTTAGAAGATATCCCAATACCTATGAATCCATCATTGCAACTCTATGCGAAAGCCTAGACACTTTAGATGAACCAGAAGCCAAGGCATCGATGATCTGGATAATTGGTGAATATGCGGAAAGAATTGACAATGCTGATGAGCTCCTTGAAAGCTTTCTGGAAAGTTTCCCTGAAGAACCTGCACATGTCCAGTTGCAGTTGCTGACTGCAACTGTCAAACTTTTTCTTAAAAAGCCCACCGAAGGACCACAACAGATGATTCAGGTTGTTCTGAATAATGCTACTGTGGAGACAGACAATCCTGATCTGAGAGATCGTGCATACATATATTGGCGCCTTCTATCAACTGATCCTGAGGCAGCCAAGGATGTTGTTTTAGCTGAAAAGCCAGTGATCAGTGACGATTCAAACCTGCTTGATTCATCACTTCTGGATGAACTTCTTGCAAACATTGCTACGTTATCCTCTGTGTATCACAAGCCCCCAGAAGCATTTGTTACCCGTGTGAAGACCTCAACACCGAGACCTGAAGATGAGGAGTATGGTAGCGAAACAGGAAATTCTGAATCAGCTGCTGATAGTGCAGCTACTGGTGCATCCCCACCACCAACTAGTTCAGGTAGTGTTCCATATGCTGCACAAAGGCAACCAGTCCCTGCACCAGCTTCGCCTGCTGCTCCAGTGCCAGATTTACTTGGTGATCTGATTGGCCTGGATAACAGTGCCATTGTCCCTGTGGATCAGCCTGCTGCTCCTACTGGACCTCCGTTGCCTGTCCTACTACCAGCATCAACCGGTCAAGGTTTGCAAATCAGTGCTCAGTTGACACGCAGGGATGGTCAAATATTTTACAGTATATTGTTTGAGAACAACACACATGTCCCATTAGATGGGTTTATGATTCAGTTCAACAAAAATACTTTCGGTCTTGCAGCTGCTGGACCCCTTCAGGTTCCACAAATTCAACCTGGGACATCAGCAGGGACTCTCCTGCCTATGGTTACGTTCCAGAATATGTCTCAAGGTCCTCCAAGCTCATTATTGCAGGTTGCTGTGAAAAACAATCAACAACCTGTCTGGTACTTCAACGATAAAATCCCATTGCATGCCTTCTTTACCGAGGATGGCAGAATGGAGCGTGCAAATTTTCTTGAGACTTGGAGATCCCTTCCTGATTCAAATGAGATTACAAAAGACTTCCCTGGAATTGTGGTGGGTAATGTTGAAGCAACTCTGGACCGCCTGGCTGCTACAAACATGTTCTTTATTGCGAAGCGAAAACACGCCAACCAGGATGTCTTCTATTTCTCTGCAAAGATACCCCGAGGAATACCCTTCCTAATTGAACTCACTACAGTAGTAAATACCCCTGGGGTCAAGTGCGCAATCAAGACTCCAAGCCCAGAATCAGCACCCCTTTTCTTTGAAGCTATTCCACCCACCCCAAAGATGCCATACACGAGAGCAAAAAGGTCTGCTTCACCACAGACCTCTGTCTCACCTGTCAAAGGCCGCCGATCGAGATCATTGTCGAGGTCTAGAAGGAGTCGTTCTAGGAGCCCATCTGTTGATGCAACCAATCCTGGAAATAACTTGTATGTAACAGGCTTATCCACAAGGGTTACCTCTGCTGATGTTGAGAAATTCTTTAACAAAGAAGGCAAGGTGTTGGAGTGCCATCTTGTAACTGACCCTCGCTCCAGAGAATCTCGTGGATTTGCATTTGTCACAATGGAAACTGTAGAGGATGCAGAGCGATGCATTAAGTACTTGAATCGTTCTGTGCTTGAAGGTCGACTAGTCACTGTGGAAAAGGCGAAGAGGAAGCGAGGGAGGACACCTACTCCAGGAAGGTATCAAGGCTTGAGAGATAAGCGAGATGCAGGACGTGATCGTGGAGATCGTGATTATGGTGATCGTGACCGTGACCGTGACCGTGATCGTAGTCGTAGAAGATCTCGTAGCTATTCACCTCGTAGGCGGAAAGACAGAGAGCCTCCTTCCAGGGACAACCGCCGAGGAAGATCACGTTCTCCACCAGCTAGGGATAGGAGGGTGGATGAACATTCAGACTCGTATAGGAGGCGCATGGATCACTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

1118

Amino Acids

125.22

Weight (kDa)

8.46

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 14 - 534 2.7e-169 Adaptin N terminal region
HEAT_2 PF13646 98 - 152 3e-06 HEAT repeats
Cnd1 PF12717 103 - 266 2.8e-65 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 680 - 779 5e-11 Adaptin C-terminal domain
B2-adapt-app_C PF09066 790 - 895 1.4e-29 Beta2-adaptin appendage, C-terminal sub-domain
RRM_1 PF00076 949 - 1018 1.1e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2635
Acc36I ACCTGC 3 cut(s) 1438, 1671, 2287
AccB1I GGYRCC 1 cut(s) 1584
AccB7I CCANNNNNTGG 2 cut(s) 802, 2141
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 3046
AccII CGCG 1 cut(s) 742
AciI CCGC 7 cut(s) 6, 740, 1367, 2491, 2764, 3232, 3265
AclWI GGATC 8 cut(s) 371, 401, 784, 983, 1595, 1998, 2409, 3351
AcsI RAATTY 7 cut(s) 48, 190, 564, 1810, 2211, 2395, 2894
AcuI CTGAAG 8 cut(s) 221, 368, 521, 1023, 1110, 1443, 1800, 2183
AcyI GRCGYC 1 cut(s) 1585
AdeI CACNNNGTG 1 cut(s) 651
AfaI GTAC 5 cut(s) 23, 820, 983, 2333, 3020
AfiI CCNNNNNNNGG 6 cut(s) 802, 1493, 2141, 2248, 2759, 3098
AflII CTTAAG 1 cut(s) 395
AflIII ACRYGT 3 cut(s) 1435, 2128, 2508
AgeI ACCGGT 1 cut(s) 2045
AhlI ACTAGT 2 cut(s) 1862, 3049
AjiI CACGTC 1 cut(s) 3140
Alw21I GWGCWC 4 cut(s) 60, 72, 1395, 2071
Alw26I GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2752
AlwI GGATC 8 cut(s) 371, 401, 784, 983, 1595, 1998, 2409, 3351
AlwNI CAGNNNCTG 5 cut(s) 1453, 1826, 1841, 1907, 2189
Ama87I CYCGRG 2 cut(s) 53, 2574
AoxI GGCC 4 cut(s) 938, 1227, 1960, 2761
ApoI RAATTY 7 cut(s) 48, 190, 564, 1810, 2211, 2395, 2894
AsiGI ACCGGT 1 cut(s) 2045
Asp700I GAANNNNTTC 2 cut(s) 1404, 2682
AspLEI GCGC 4 cut(s) 556, 1587, 2636, 3339
AspS9I GGNCC 7 cut(s) 295, 939, 1496, 2012, 2192, 2276, 2488
AsuHPI GGTGA 9 cut(s) 56, 127, 805, 1370, 1961, 2724, 2742, 3176, 3213
AvaI CYCGRG 2 cut(s) 53, 2574
AvaII GGWCC 6 cut(s) 295, 1496, 2012, 2192, 2276, 2488
AxyI CCTNAGG 1 cut(s) 1605
BaeGI GKGCMC 1 cut(s) 12
BanI GGYRCC 1 cut(s) 1584
BanII GRGCYC 4 cut(s) 60, 72, 1395, 2813
BauI CACGAG 3 cut(s) 863, 2710, 2961
BbsI GAAGAC 2 cut(s) 1766, 2542
Bbv12I GWGCWC 4 cut(s) 60, 72, 1395, 2071
BceAI ACGGC 2 cut(s) 308, 953
BcgI CGANNNNNNTGC 4 cut(s) 443, 477, 1899, 1933
BclI TGATCA 1 cut(s) 1644
BcoDI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2752
BcuI ACTAGT 2 cut(s) 1862, 3049
BfaI CTAG 8 cut(s) 254, 684, 1307, 1863, 2790, 2805, 3050, 3294
BfmI CTRYAG 3 cut(s) 746, 2604, 2991
BfoI RGCGCY 1 cut(s) 1588
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 3 cut(s) 1438, 1671, 2287
BglII AGATCT 1 cut(s) 3206
BlpI GCTNAGC 1 cut(s) 882
BmcAI AGTACT 2 cut(s) 23, 3020
Bme18I GGWCC 6 cut(s) 295, 1496, 2012, 2192, 2276, 2488
BmeT110I CYCGRG 2 cut(s) 53, 2574
BmgBI CACGTC 1 cut(s) 3140
BmgT120I GGNCC 7 cut(s) 295, 939, 1496, 2012, 2192, 2276, 2488
BmiI GGNNCC 6 cut(s) 297, 855, 1586, 2194, 2205, 2810
BoxI GACNNNNGTC 1 cut(s) 2744
BpiI GAAGAC 2 cut(s) 1766, 2542
BpmI CTGGAG 3 cut(s) 1914, 2935, 3081
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 4 cut(s) 1208, 2256, 2423, 3136
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 1 cut(s) 1216
BsaBI GATNNNNATC 4 cut(s) 405, 522, 1199, 2773
BsaHI GRCGYC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 1768
BsaWI WCCGGW 1 cut(s) 2045
BsaXI ACNNNNNCTCC 6 cut(s) 1999, 2029, 2933, 2963, 3079, 3109
Bsc4I CCNNNNNNNGG 6 cut(s) 802, 1493, 2141, 2248, 2759, 3098
Bse118I RCCGGY 1 cut(s) 2045
Bse1I ACTGG 9 cut(s) 603, 675, 816, 1441, 1640, 1846, 1901, 1931, 2014
Bse21I CCTNAGG 1 cut(s) 1605
Bse3DI GCAATG 4 cut(s) 132, 1284, 1701, 2351
Bse8I GATNNNNATC 4 cut(s) 405, 522, 1199, 2773
BseCI ATCGAT 1 cut(s) 1340
BseJI GATNNNNATC 4 cut(s) 405, 522, 1199, 2773
BseLI CCNNNNNNNGG 6 cut(s) 802, 1493, 2141, 2248, 2759, 3098
BseMI GCAATG 4 cut(s) 132, 1284, 1701, 2351
BseMII CTCAG 6 cut(s) 456, 873, 879, 1550, 1596, 2084
BseNI ACTGG 9 cut(s) 603, 675, 816, 1441, 1640, 1846, 1901, 1931, 2014
BseRI GAGGAG 2 cut(s) 846, 1802
BseSI GKGCMC 1 cut(s) 12
BsgI GTGCAG 4 cut(s) 1416, 1854, 1872, 1893
Bsh1236I CGCG 1 cut(s) 742
Bsh1285I CGRYCG 1 cut(s) 2771
BshFI GGCC 4 cut(s) 940, 1229, 1962, 2763
BshNI GGYRCC 1 cut(s) 1584
BshTI ACCGGT 1 cut(s) 2045
BshVI ATCGAT 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 2771
BsiHKAI GWGCWC 4 cut(s) 60, 72, 1395, 2071
BsiHKCI CYCGRG 2 cut(s) 53, 2574
BsiSI CCGG 1 cut(s) 2046
BslFI GGGAC 7 cut(s) 669, 1889, 1967, 2117, 2236, 2248, 3272
BslI CCNNNNNNNGG 6 cut(s) 802, 1493, 2141, 2248, 2759, 3098
BsmAI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2752
BsmFI GGGAC 7 cut(s) 669, 1889, 1967, 2117, 2236, 2248, 3272
BsmI GAATGC 2 cut(s) 342, 647
BsnI GGCC 4 cut(s) 940, 1229, 1962, 2763
Bso31I GGTCTC 1 cut(s) 1768
BsoBI CYCGRG 2 cut(s) 53, 2574
Bsp1286I GDGCHC 6 cut(s) 12, 60, 72, 1395, 2071, 2813
Bsp1720I GCTNAGC 1 cut(s) 882
BspACI CCGC 7 cut(s) 6, 740, 1367, 2491, 2764, 3232, 3265
BspANI GGCC 4 cut(s) 940, 1229, 1962, 2763
BspCNI CTCAG 6 cut(s) 457, 874, 880, 1551, 1597, 2083
BspDI ATCGAT 1 cut(s) 1340
BspFNI CGCG 1 cut(s) 742
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 6 cut(s) 297, 855, 1586, 2194, 2205, 2810
BspMI ACCTGC 3 cut(s) 1438, 1671, 2287
BspPI GGATC 8 cut(s) 371, 401, 784, 983, 1595, 1998, 2409, 3351
BspQI GCTCTTC 1 cut(s) 60
BspT107I GGYRCC 1 cut(s) 1584
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 1768
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 132, 1284, 1701, 2351
BsrFI RCCGGY 1 cut(s) 2045
BsrI ACTGG 9 cut(s) 603, 675, 816, 1441, 1640, 1846, 1901, 1931, 2014
BssAI RCCGGY 1 cut(s) 2045
BssNI GRCGYC 1 cut(s) 1585
BssSI CACGAG 3 cut(s) 863, 2710, 2961
BssT1I CCWWGG 2 cut(s) 1332, 1614
Bst2BI CACGAG 3 cut(s) 863, 2710, 2961
Bst6I CTCTTC 2 cut(s) 60, 3065
BstACI GRCGYC 1 cut(s) 1585
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 3 cut(s) 263, 701, 1703
BstBAI YACGTR 1 cut(s) 1216
BstDEI CTNAG 6 cut(s) 465, 882, 888, 1559, 1605, 2070
BstEII GGTNACC 1 cut(s) 2872
BstENI CCTNNNNNAGG 2 cut(s) 2757, 3096
BstFNI CGCG 1 cut(s) 742
BstH2I RGCGCY 1 cut(s) 1588
BstHHI GCGC 4 cut(s) 556, 1587, 2636, 3339
BstMAI GTCTC 6 cut(s) 151, 1535, 1768, 2274, 2399, 2752
BstMCI CGRYCG 1 cut(s) 2771
BstNSI RCATGY 6 cut(s) 181, 755, 1439, 2132, 2360, 2512
BstPAI GACNNNNGTC 1 cut(s) 2744
BstPI GGTNACC 1 cut(s) 2872
BstSFI CTRYAG 3 cut(s) 746, 2604, 2991
BstSLI GKGCMC 1 cut(s) 12
BstSNI TACGTA 1 cut(s) 1216
BstUI CGCG 1 cut(s) 742
BstV2I GAAGAC 2 cut(s) 1766, 2542
BstX2I RGATCY 2 cut(s) 2414, 3206
BstYI RGATCY 2 cut(s) 2414, 3206
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 1605
BsuRI GGCC 4 cut(s) 940, 1229, 1962, 2763
BsuTUI ATCGAT 1 cut(s) 1340
BtgZI GCGATG 1 cut(s) 3022
BtrI CACGTC 1 cut(s) 3140
BtsIMutI CAGTG 6 cut(s) 1647, 1654, 1938, 1978, 2071, 3054
BveI ACCTGC 3 cut(s) 1438, 1671, 2287
CaiI CAGNNNCTG 5 cut(s) 1453, 1826, 1841, 1907, 2189
CciI TCATGA 1 cut(s) 955
CfoI GCGC 4 cut(s) 556, 1587, 2636, 3339
Cfr10I RCCGGY 1 cut(s) 2045
Cfr13I GGNCC 7 cut(s) 295, 939, 1496, 2012, 2192, 2276, 2488
ClaI ATCGAT 1 cut(s) 1340
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 5 cut(s) 22, 819, 982, 2332, 3019
CspAI ACCGGT 1 cut(s) 2045
CspCI CAANNNNNGTGG 4 cut(s) 528, 563, 931, 966
CviAII CATG 8 cut(s) 178, 752, 956, 1436, 2129, 2357, 2509, 3340
CviQI GTAC 5 cut(s) 22, 819, 982, 2332, 3019
DdeI CTNAG 6 cut(s) 465, 882, 888, 1559, 1605, 2070
DinI GGCGCC 1 cut(s) 1586
DraI TTTAAA 1 cut(s) 1075
DraIII CACNNNGTG 1 cut(s) 651
Eam1104I CTCTTC 2 cut(s) 60, 3065
EarI CTCTTC 2 cut(s) 60, 3065
EciI GGCGGA 1 cut(s) 3247
Ecl136II GAGCTC 3 cut(s) 58, 70, 1393
Eco105I TACGTA 1 cut(s) 1216
Eco130I CCWWGG 2 cut(s) 1332, 1614
Eco24I GRGCYC 4 cut(s) 60, 72, 1395, 2813
Eco31I GGTCTC 1 cut(s) 1768
Eco32I GATATC 2 cut(s) 1249, 1262
Eco47I GGWCC 6 cut(s) 295, 1496, 2012, 2192, 2276, 2488
Eco53kI GAGCTC 3 cut(s) 58, 70, 1393
Eco57I CTGAAG 8 cut(s) 221, 368, 521, 1023, 1110, 1443, 1800, 2183
Eco81I CCTNAGG 1 cut(s) 1605
Eco88I CYCGRG 2 cut(s) 53, 2574
Eco91I GGTNACC 1 cut(s) 2872
EcoICRI GAGCTC 3 cut(s) 58, 70, 1393
EcoNI CCTNNNNNAGG 2 cut(s) 2757, 3096
EcoO109I RGGNCCY 2 cut(s) 295, 2276
EcoO65I GGTNACC 1 cut(s) 2872
EcoRV GATATC 2 cut(s) 1249, 1262
EcoT14I CCWWGG 2 cut(s) 1332, 1614
EcoT22I ATGCAT 2 cut(s) 344, 3014
EcoT38I GRGCYC 4 cut(s) 60, 72, 1395, 2813
EgeI GGCGCC 1 cut(s) 1586
EheI GGCGCC 1 cut(s) 1586
ErhI CCWWGG 2 cut(s) 1332, 1614
FaeI CATG 8 cut(s) 181, 755, 959, 1439, 2132, 2360, 2512, 3343
FalI AAGNNNNNCTT 4 cut(s) 956, 988, 2713, 2745
FaqI GGGAC 7 cut(s) 669, 1889, 1967, 2117, 2236, 2248, 3272
FatI CATG 8 cut(s) 177, 751, 955, 1435, 2128, 2356, 2508, 3339
FauI CCCGC 1 cut(s) 747
FauNDI CATATG 3 cut(s) 412, 434, 1882
FbaI TGATCA 1 cut(s) 1644
FblI GTMKAC 1 cut(s) 3046
FriOI GRGCYC 4 cut(s) 60, 72, 1395, 2813
FspBI CTAG 8 cut(s) 254, 684, 1307, 1863, 2790, 2805, 3050, 3294
FspI TGCGCA 1 cut(s) 2635
GlaI GCGC 4 cut(s) 555, 1586, 2635, 3338
GsuI CTGGAG 3 cut(s) 1914, 2935, 3081
HaeII RGCGCY 1 cut(s) 1588
HaeIII GGCC 4 cut(s) 940, 1229, 1962, 2763
HapII CCGG 1 cut(s) 2046
HhaI GCGC 4 cut(s) 556, 1587, 2636, 3339
Hin1I GRCGYC 1 cut(s) 1585
Hin1II CATG 8 cut(s) 181, 755, 959, 1439, 2132, 2360, 2512, 3343
Hin6I GCGC 4 cut(s) 554, 1585, 2634, 3337
HinP1I GCGC 4 cut(s) 554, 1585, 2634, 3337
HincII GTYRAC 2 cut(s) 2076, 3047
HindII GTYRAC 2 cut(s) 2076, 3047
HindIII AAGCTT 2 cut(s) 1031, 1402
HpaII CCGG 1 cut(s) 2046
HphI GGTGA 9 cut(s) 56, 127, 805, 1370, 1961, 2724, 2742, 3176, 3213
Hpy166II GTNNAC 2 cut(s) 2076, 3047
Hpy8I GTNNAC 2 cut(s) 2076, 3047
HpyCH4IV ACGT 5 cut(s) 1215, 1709, 2255, 3139, 3280
HpyF3I CTNAG 6 cut(s) 465, 882, 888, 1559, 1605, 2070
HpySE526I ACGT 5 cut(s) 1215, 1709, 2255, 3139, 3280
Hsp92I GRCGYC 1 cut(s) 1585
Hsp92II CATG 8 cut(s) 181, 755, 959, 1439, 2132, 2360, 2512, 3343
HspAI GCGC 4 cut(s) 554, 1585, 2634, 3337
KasI GGCGCC 1 cut(s) 1584
Ksp22I TGATCA 1 cut(s) 1644
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 9 cut(s) 196, 627, 859, 1398, 1933, 2008, 2386, 2808, 2954
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 8 cut(s) 254, 684, 1307, 1863, 2790, 2805, 3050, 3294
MaeII ACGT 5 cut(s) 1215, 1709, 2255, 3139, 3280
MbiI CCGCTC 1 cut(s) 6
MflI RGATCY 2 cut(s) 2414, 3206
MhlI GDGCHC 6 cut(s) 12, 60, 72, 1395, 2071, 2813
Mly113I GGCGCC 1 cut(s) 1585
MlyI GAGTC 5 cut(s) 8, 2230, 2638, 2806, 3317
MmeI TCCRAC 2 cut(s) 820, 2901
Mph1103I ATGCAT 2 cut(s) 344, 3014
MroXI GAANNNNTTC 2 cut(s) 1404, 2682
MseI TTAA 8 cut(s) 108, 396, 963, 1074, 1197, 1479, 2901, 3015
MspA1I CMGCKG 5 cut(s) 698, 779, 1163, 1823, 2186
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 1 cut(s) 2046
Mva1269I GAATGC 2 cut(s) 342, 647
MvnI CGCG 1 cut(s) 742
NarI GGCGCC 1 cut(s) 1585
NdeI CATATG 3 cut(s) 412, 434, 1882
NlaIII CATG 8 cut(s) 181, 755, 959, 1439, 2132, 2360, 2512, 3343
NlaIV GGNNCC 6 cut(s) 297, 855, 1586, 2194, 2205, 2810
NmeAIII GCCGAG 1 cut(s) 3293
NmuCI GTSAC 8 cut(s) 605, 865, 1649, 2977, 3052, 3170, 3176, 3182
NsbI TGCGCA 1 cut(s) 2635
NsiI ATGCAT 2 cut(s) 344, 3014
NspI RCATGY 6 cut(s) 181, 755, 1439, 2132, 2360, 2512
PaeI GCATGC 3 cut(s) 181, 755, 2360
PagI TCATGA 1 cut(s) 955
PasI CCCWGGG 1 cut(s) 2621
PciI ACATGT 3 cut(s) 1435, 2128, 2508
PciSI GCTCTTC 1 cut(s) 60
PcsI WCGNNNNNNNCGW 1 cut(s) 3151
PctI GAATGC 2 cut(s) 342, 647
PdmI GAANNNNTTC 2 cut(s) 1404, 2682
PflMI CCANNNNNTGG 2 cut(s) 802, 2141
PfoI TCCNGGA 3 cut(s) 489, 2833, 3097
PinAI ACCGGT 1 cut(s) 2045
Ple19I CGATCG 1 cut(s) 2771
PleI GAGTC 5 cut(s) 8, 2230, 2638, 2805, 3317
PluTI GGCGCC 1 cut(s) 1588
PpsI GAGTC 5 cut(s) 8, 2230, 2638, 2805, 3317
Ppu21I YACGTR 1 cut(s) 1216
PpuMI RGGWCCY 2 cut(s) 295, 2276
PscI ACATGT 3 cut(s) 1435, 2128, 2508
PshAI GACNNNNGTC 1 cut(s) 2744
Psp124BI GAGCTC 3 cut(s) 60, 72, 1395
Psp5II RGGWCCY 2 cut(s) 295, 2276
PspEI GGTNACC 1 cut(s) 2872
PspN4I GGNNCC 6 cut(s) 297, 855, 1586, 2194, 2205, 2810
PspPI GGNCC 7 cut(s) 295, 939, 1496, 2012, 2192, 2276, 2488
PspPPI RGGWCCY 2 cut(s) 295, 2276
PstNI CAGNNNCTG 5 cut(s) 1453, 1826, 1841, 1907, 2189
PsuI RGATCY 2 cut(s) 2414, 3206
PvuI CGATCG 1 cut(s) 2771
PvuII CAGCTG 5 cut(s) 698, 779, 1163, 1823, 2186
RsaI GTAC 5 cut(s) 23, 820, 983, 2333, 3020
RsaNI GTAC 5 cut(s) 22, 819, 982, 2332, 3019
SacI GAGCTC 3 cut(s) 60, 72, 1395
SalI GTCGAC 1 cut(s) 3045
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 8 cut(s) 108, 396, 963, 1074, 1197, 1479, 2901, 3015
Sau96I GGNCC 7 cut(s) 295, 939, 1496, 2012, 2192, 2276, 2488
ScaI AGTACT 2 cut(s) 23, 3020
SchI GAGTC 5 cut(s) 8, 2230, 2638, 2806, 3317
SduI GDGCHC 6 cut(s) 12, 60, 72, 1395, 2071, 2813
SexAI ACCWGGT 1 cut(s) 1056
SfcI CTRYAG 3 cut(s) 746, 2604, 2991
SfoI GGCGCC 1 cut(s) 1586
SinI GGWCC 6 cut(s) 295, 1496, 2012, 2192, 2276, 2488
SmlI CTYRAG 5 cut(s) 395, 1187, 2271, 2402, 3115
SmoI CTYRAG 5 cut(s) 395, 1187, 2271, 2402, 3115
SnaBI TACGTA 1 cut(s) 1216
SpeI ACTAGT 2 cut(s) 1862, 3049
SphI GCATGC 3 cut(s) 181, 755, 2360
SsiI CCGC 7 cut(s) 6, 740, 1367, 2491, 2764, 3232, 3265
SspDI GGCGCC 1 cut(s) 1584
SspI AATATT 1 cut(s) 2097
SspMI CTAG 8 cut(s) 254, 684, 1307, 1863, 2790, 2805, 3050, 3294
SstI GAGCTC 3 cut(s) 60, 72, 1395
StyI CCWWGG 2 cut(s) 1332, 1614
TaiI ACGT 5 cut(s) 1218, 1712, 2258, 3142, 3283
TaqI TCGA 5 cut(s) 1045, 1340, 2771, 2783, 3046
TaqII GACCGA 1 cut(s) 2165
TatI WGTACW 3 cut(s) 21, 981, 3018
TauI GCSGC 1 cut(s) 2766
Tru1I TTAA 8 cut(s) 108, 396, 963, 1074, 1197, 1479, 2901, 3015
Tru9I TTAA 8 cut(s) 108, 396, 963, 1074, 1197, 1479, 2901, 3015
TscAI CASTG 6 cut(s) 1647, 1654, 1938, 1978, 2071, 3061
TseFI GTSAC 8 cut(s) 605, 865, 1649, 2977, 3052, 3170, 3176, 3182
Tsp45I GTSAC 8 cut(s) 605, 865, 1649, 2977, 3052, 3170, 3176, 3182
TspDTI ATGAA 7 cut(s) 654, 972, 1290, 1335, 1662, 1701, 3327
TspGWI ACGGA 3 cut(s) 176, 1112, 2007
TspRI CASTG 6 cut(s) 1647, 1654, 1938, 1978, 2071, 3061
Van91I CCANNNNNTGG 2 cut(s) 802, 2141
Vha464I CTTAAG 1 cut(s) 395
VpaK11BI GGWCC 6 cut(s) 295, 1496, 2012, 2192, 2276, 2488
XagI CCTNNNNNAGG 2 cut(s) 2757, 3096
XapI RAATTY 7 cut(s) 48, 190, 564, 1810, 2211, 2395, 2894
XbaI TCTAGA 2 cut(s) 683, 2789
XceI RCATGY 6 cut(s) 181, 755, 1439, 2132, 2360, 2512
XcmI CCANNNNNNNNNTGG 1 cut(s) 1984
XmiI GTMKAC 1 cut(s) 3046
XmnI GAANNNNTTC 2 cut(s) 1404, 2682
XspI CTAG 8 cut(s) 254, 684, 1307, 1863, 2790, 2805, 3050, 3294
ZrmI AGTACT 2 cut(s) 23, 3020
Zsp2I ATGCAT 2 cut(s) 344, 3014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.