Rorug07G0037500

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006717
Physical Location & Seq
Reverse (-)
2637496 .. 2653186
15691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0037500.1

Sequence Viewer

Length: 699 bp
ATGGCAGAGCAAGACAAGGTGATCCTACACGGAATGTGGGCTAGTCCCTTAACAAAGAGGGTGGAATTTGCCCTCAAAGTGAAGGGTATACCCTATGAATATGTGGAAGAAGATTTGAGTAACAAAAGTCCATTGCTCCTCAAGTTCAACCCTGTTCATAAGAAGGTTCCTGTGCTTGTCCACAATGGGAAAGCCATTGCTGAGTCTCTTGTGATCATTGAATATATTGATGAAACCTGGAAAACTGGCCCTCAACTTTTACCAGAAGATCCATACAAACGATCCCAAATTCGTTTCTGGGCTAGCTATATGCAGCAGGTGTTTGAGTCCTTGGTGTTAGTGATCAAAAGCAGTGGAGAAGCACAAGAGAAAGCCATCAAAGAAGTGTCTGAGAAGGTAAAATTACTTGAAACCGGACTCAAGGGTTTGTTCCCAGATGGCATTCCTTCAGTAGATGAATTCAGCAAAAATGTGACACTACTAGATTTGTTCGTTCTCGCACACTTTGGCGAATATGAAGCTCAGGAAGAAGTCCTTGGCCTAAAGCTTATCGACACCGATAAGACTCCACTGATAGTTTCCTGCATAACAGCTCTGATTGAGATCCCTGCGGTGAAAAAGTCGCGGATTCCTCATGAAAAGATGGTGGCCTTCCTCAAGTCCTATAGAGAAGATGCCCTCAAGTCTGCTACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

232

Amino Acids

26.15

Weight (kDa)

6.02

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 7 - 78 1.9e-18 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 9 - 79 2.6e-17 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 79 1.4e-15 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 88
AccII CGCG 1 cut(s) 625
AciI CCGC 2 cut(s) 611, 625
AclWI GGATC 4 cut(s) 16, 263, 276, 598
AcsI RAATTY 3 cut(s) 65, 288, 458
AcuI CTGAAG 1 cut(s) 432
AgsI TTSAA 3 cut(s) 148, 221, 410
AjnI CCWGG 1 cut(s) 236
AjuI GAANNNNNNNTTGG 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 306, 521, 547, 593, 695
AluI AGCT 5 cut(s) 306, 521, 547, 593, 695
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 4 cut(s) 16, 263, 276, 598
AoxI GGCC 3 cut(s) 247, 538, 648
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 3 cut(s) 65, 288, 458
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 31, 625
AsuNHI GCTAGC 1 cut(s) 302
BbvI GCAGC 1 cut(s) 325
BccI CCATC 3 cut(s) 383, 431, 637
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 2 cut(s) 213, 342
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 3 cut(s) 42, 303, 482
BfmI CTRYAG 2 cut(s) 664, 690
BfuAI ACCTGC 1 cut(s) 307
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 664
BmtI GCTAGC 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 4 cut(s) 125, 404, 641, 665
BsaBI GATNNNNATC 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 330, 535
BsaWI WCCGGW 1 cut(s) 413
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 2 cut(s) 131, 195
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 2 cut(s) 330, 535
BseJI GATNNNNATC 1 cut(s) 602
BseMI GCAATG 2 cut(s) 131, 195
BseMII CTCAG 3 cut(s) 192, 381, 536
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 625
BshFI GGCC 3 cut(s) 249, 540, 650
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 3 cut(s) 249, 540, 650
Bsp143I GATC 6 cut(s) 21, 213, 268, 281, 342, 603
BspACI CCGC 2 cut(s) 611, 625
BspANI GGCC 3 cut(s) 249, 540, 650
BspCNI CTCAG 3 cut(s) 193, 382, 535
BspFNI CGCG 1 cut(s) 625
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 168
BspMI ACCTGC 1 cut(s) 307
BspOI GCTAGC 1 cut(s) 306
BspPI GGATC 4 cut(s) 16, 263, 276, 598
BsrDI GCAATG 2 cut(s) 131, 195
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 2 cut(s) 330, 535
BssMI GATC 6 cut(s) 21, 213, 268, 281, 342, 603
BssNAI GTATAC 1 cut(s) 89
BssT1I CCWWGG 2 cut(s) 330, 535
Bst1107I GTATAC 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 201, 390, 522
BstFNI CGCG 1 cut(s) 625
BstKTI GATC 6 cut(s) 24, 216, 271, 284, 345, 606
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 6 cut(s) 21, 213, 268, 281, 342, 603
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstSFI CTRYAG 2 cut(s) 664, 690
BstUI CGCG 1 cut(s) 625
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 2 cut(s) 268, 603
BstYI RGATCY 2 cut(s) 268, 603
BstZ17I GTATAC 1 cut(s) 89
BsuRI GGCC 3 cut(s) 249, 540, 650
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 358, 569
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 304
CciI TCATGA 1 cut(s) 634
Cfr13I GGNCC 1 cut(s) 248
CspCI CAANNNNNGTGG 4 cut(s) 42, 77, 334, 369
CviAII CATG 1 cut(s) 635
DdeI CTNAG 3 cut(s) 201, 390, 522
DpnI GATC 6 cut(s) 23, 215, 270, 283, 344, 605
DpnII GATC 6 cut(s) 21, 213, 268, 281, 342, 603
Eco130I CCWWGG 2 cut(s) 330, 535
Eco57I CTGAAG 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 2 cut(s) 330, 535
ErhI CCWWGG 2 cut(s) 330, 535
FaeI CATG 1 cut(s) 638
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 30
FatI CATG 1 cut(s) 634
FbaI TGATCA 2 cut(s) 213, 342
FblI GTMKAC 1 cut(s) 88
Fnu4HI GCNGC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 3 cut(s) 42, 303, 482
GluI GCNGC 1 cut(s) 314
HaeIII GGCC 3 cut(s) 249, 540, 650
HapII CCGG 1 cut(s) 414
Hin1II CATG 1 cut(s) 638
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 5 cut(s) 203, 326, 417, 565, 628
HpaII CCGG 1 cut(s) 414
HphI GGTGA 2 cut(s) 31, 625
Hpy166II GTNNAC 2 cut(s) 89, 181
Hpy188I TCNGA 2 cut(s) 391, 597
Hpy188III TCNNGA 2 cut(s) 524, 635
Hpy8I GTNNAC 2 cut(s) 89, 181
HpyAV CCTTC 5 cut(s) 76, 157, 388, 456, 661
HpyCH4V TGCA 2 cut(s) 313, 585
HpyF3I CTNAG 3 cut(s) 201, 390, 522
Hsp92II CATG 1 cut(s) 638
Ksp22I TGATCA 2 cut(s) 213, 342
Kzo9I GATC 6 cut(s) 21, 213, 268, 281, 342, 603
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 664
MaeI CTAG 3 cut(s) 42, 303, 482
MaeIII GTNAC 2 cut(s) 119, 472
MalI GATC 6 cut(s) 23, 215, 270, 283, 344, 605
MboI GATC 6 cut(s) 21, 213, 268, 281, 342, 603
MboII GAAGA 5 cut(s) 119, 122, 278, 539, 683
MflI RGATCY 2 cut(s) 268, 603
MluCI AATT 4 cut(s) 65, 288, 401, 458
MlyI GAGTC 4 cut(s) 212, 335, 411, 559
MnlI CCTC 7 cut(s) 51, 83, 149, 261, 642, 665, 689
MseI TTAA 1 cut(s) 50
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 1 cut(s) 238
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 1 cut(s) 625
NdeII GATC 6 cut(s) 21, 213, 268, 281, 342, 603
NheI GCTAGC 1 cut(s) 302
NlaIII CATG 1 cut(s) 638
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 472
PagI TCATGA 1 cut(s) 634
PaqCI CACCTGC 1 cut(s) 307
PctI GAATGC 1 cut(s) 441
PfeI GAWTC 1 cut(s) 628
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 4 cut(s) 211, 334, 411, 559
PpsI GAGTC 4 cut(s) 211, 334, 411, 559
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 2 cut(s) 268, 603
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 6 cut(s) 21, 213, 268, 281, 342, 603
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 4 cut(s) 212, 335, 411, 559
ScrFI CCNGG 1 cut(s) 238
SfaNI GCATC 1 cut(s) 664
SfcI CTRYAG 2 cut(s) 664, 690
SmlI CTYRAG 4 cut(s) 140, 419, 656, 680
SmoI CTYRAG 4 cut(s) 140, 419, 656, 680
Sse9I AATT 4 cut(s) 65, 288, 401, 458
SsiI CCGC 2 cut(s) 611, 625
SspMI CTAG 3 cut(s) 42, 303, 482
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 2 cut(s) 330, 535
TaqI TCGA 1 cut(s) 552
TasI AATT 4 cut(s) 65, 288, 401, 458
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 358, 576
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 6 cut(s) 111, 146, 246, 471, 531, 651
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 358, 576
XapI RAATTY 3 cut(s) 65, 288, 458
XmiI GTMKAC 1 cut(s) 88
XspI CTAG 3 cut(s) 42, 303, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.