FvH4_5g02830

Subunit of clathrin-associated adaptor protein complex that plays a role in protein sorting in the late-Golgi trans-Golgi network (TGN) and or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006717
Physical Location & Seq
Reverse (-)
1698527 .. 1705210
6684 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g02830.t1

Sequence Viewer

Length: 2715 bp
ATGAGCGGCCACGACTCCAAGTACTTCTCCACCACCAAGAAGGGTGAAATCCCCGAGCTCAAAGAAGAGCTCAATTCTCAATACAAGGACAAGAGGAAAGATGCGGTTAAGAAGGTGATTGCTGCAATGACAGTTGGGAAGGACGTCTCGTCGCTTTTCACGGATGTTGTCAATTGCATGCAAACCGAAAATTTGGAGCTCAAGAAGCTGGTTTATCTGTATCTGATCAATTATGCTAAAAGCCAGCCTGACCTGGCAATTCTTGCTGTCAATACATTTGTCAAGGATTCACAGGACCCAAATCCTTTGATTCGTGCTTTAGCCGTTCGGACCATGGGTTGCATTCGTGTTGATAAAATTACAGAATATTTATGTGATCCCCTTCAGAGATGCCTTAAGGATGATGATCCATATGTTCGCAAGACAGCAGCCATATGTGTCGCCAAACTTTATGACATAAATGCTGAGTTAGTTGAGGATAGGGGATTCCTGGAATCTCTCAAGGATTTGATTTCTGATAATAATCCTATGGTTGTAGCAAATGCTGTGGCTGCGCTTGCTGAAATTCAAGAGAATAGTAATAGACCCATCTTTGAGATGACCAGCCACACTCTGACAAAGCTCCTTACTGCCTTGAATGAATGCACAGAGTGGGGACAAGTATTTATACTGGACGCTCTTTCTAGATACAAGGCTGCTGATGCCCGTGAAGCTGAAAATATAGTAGAGCGAGTTACCCCACGACTACAGCATGCTAACTGTGCTGTTGTGCTTTCAGCTGTGAAGATGATCCTCCAACAAATGGAACTCATCACCAGTACTGATGTCGTCAGAAATCTTTGTAAAAAGATGGCTCCTCCACTTGTGACACTACTCTCTGCTGAGCCTGAGATACAATATGTTGCATTGAGAAACATCAACCTTATAGTACAGAGACGGCCCACAATTCTTGCTCATGAAATTAAGGTATTCTTTTGCAAGTACAATGATCCTATTTATGTAAAGATGGAAAAGTTAGAAATTATGATAAAGCTTGCTTCAGATAGAAACATAGACCAGGTTCTGTTGGAGTTTAAAGAGTATGCTACTGAAGTAGATGTGGATTTTGTCAGAAAGGCTGTCCGTGCCATTGGTCGCTGTGCCATCAAATTAGAAAGAGCAGCTGAACGGTGCATTACTGTATTACTTGAGTTGATTAAGATCAAAGTCAATTACGTAGTTCAAGAGGCCATTATTGTCATCAAAGACATCTTTAGAAGATATCCCAATACCTATGAATCCATCATCGCTACTCTATGTGAAAGCCTAGACACTTTAGATGAACCAGAAGCCAAGGCATCGATGATCTGGATAATTGGTGAATATGCGGAAAGAATTGACAATGCCGATGAGCTACTTGAAAGCTTTCTGGAAAGTTTCCCTGAAGAACCTGCACATGTCCAGTTGCAGTTGCTGACTGCAACTGTCAAACTTTTTCTTAAAAAGCCTACCGAAGGACCACAACAGATGATTCAGGTTGTTTTGAATAATGCTACTGTGGAGACAGACAATCCTGATCTGAGAGATCGTGCATACATCTATTGGCGCCTTCTATCAACTGATCCTGAGGCAGCAAAGGATGTTGTTTTAGCTGAGAAGCCAGTGATCAGTGACGATTCAAACCTCCTTGATTCCTCACTTCTTGATGAACTTCTTGCAAACATTGCTACATTGTCCTCTGTGTATCACAAGCCCCCAGAAGCATTTGTTACTCGTGTGAAGACCTCAACATCGAGACCTGAAGATGAGGAGTATGGTAGTGAAACAGGAAATTCTGAATCACCTGCTCATCTTGCTGATAGCGCTGCTACTGGTTCATCCCCACCACCAACTAGTTCAGGTAGTGTTCCATATGGTGCACAAAGGCAACCAGCCCCTGCACCAGCTTCAGCTGCTGCTCCAGTGCCAGATTTACTTGGTGATCTGATTGGCCTGGATAATAGCGCTATTGTCCCTGTGGATCAGCCTGCTGCTCCTACCGGGCCTCCATTGCCTGTCTTAGTACCAGCATCAACTGGTCAAGGTTTACAAATCAGTGCTCAGTTGACACGCAGGGATGCTCAAATTTATTACAGTATATTGTTTGAGAACAACACACAAGTCCCATTAGATGGGTTCATGATTCAGTTCAACAAAAATACTTTTGGTCTTGCGGCTGCTGGTCCCCTTCAGGTTCCACAAATTCAACCCGGGACATCAGCAGGGACTCTCCTGCCTATGGTTACATTCCAGAATATGTCTCAAGGTCCTCCAAGCTCATTATTGCAGGTTGCTGTGAAAAACAATCAACAACCTGTGTGGTACTTCAATGATAAAATCCCACTGCATATCTTCTTTACCGAGGATGGCAGAATGGAGCGTGCAAACTTCCTTGAGACATGGAGGTCCCTTCCTGATTCAAATGAGATTACTAAAGAGTTCCCTGGAATTGTGGTGAGCAATGTTGAAGCAACTCTGGATCGCCTGGCTGCTACAAACATGTTTTTTATTGCAAAGCGAAAACACGCCAACCAGGATGTCTTTTATTTCTCTGCAAATATACCCAGAGGAATACCATTCCTAATTGAAATGACTACAGTAGTCAATACCCCTGGGGTCAAGTGCGCAATCAAGACTCCAAGCCCAGAGTCGGCACCCCTTTTCTTTGAAGCTGTGGAGACTCTCCTGAAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N Alpha_adaptinC2 B2-adapt-app_C

Protein Analysis

905

Amino Acids

100.39

Weight (kDa)

5.07

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 14 - 534 1.6e-169 Adaptin N terminal region
HEAT_2 PF13646 98 - 152 2.6e-06 HEAT repeats
Cnd1 PF12717 103 - 266 2e-65 non-SMC mitotic condensation complex subunit 1
Alpha_adaptinC2 PF02883 681 - 782 1.4e-10 Adaptin C-terminal domain
B2-adapt-app_C PF09066 793 - 902 1.8e-30 Beta2-adaptin appendage, C-terminal sub-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1831
AasI GACNNNNNNGTC 1 cut(s) 2422
AatII GACGTC 1 cut(s) 147
Acc16I TGCGCA 1 cut(s) 2644
Acc36I ACCTGC 3 cut(s) 1438, 1831, 2296
AccB1I GGYRCC 2 cut(s) 1584, 2671
AccB7I CCANNNNNTGG 2 cut(s) 802, 2150
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 4 cut(s) 6, 104, 1367, 2192
AclWI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 2007, 2505
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 5 cut(s) 190, 564, 1810, 2103, 2220
AcuI CTGAAG 7 cut(s) 368, 1023, 1110, 1443, 1800, 1911, 2192
AcyI GRCGYC 2 cut(s) 144, 1585
AdeI CACNNNGTG 1 cut(s) 651
AfaI GTAC 6 cut(s) 23, 820, 930, 983, 2043, 2342
AfeI AGCGCT 2 cut(s) 1843, 1984
AfiI CCNNNNNNNGG 5 cut(s) 802, 1493, 2150, 2257, 2668
AflII CTTAAG 1 cut(s) 395
AflIII ACRYGT 2 cut(s) 1435, 2517
AhdI GACNNNNNGTC 1 cut(s) 148
AhlI ACTAGT 1 cut(s) 1871
AjnI CCWGG 8 cut(s) 252, 489, 1056, 1971, 2461, 2502, 2550, 2629
Alw21I GWGCWC 5 cut(s) 60, 72, 201, 1900, 2080
Alw26I GTCTC 7 cut(s) 151, 928, 1535, 1768, 2283, 2408, 2690
Alw44I GTGCAC 1 cut(s) 1896
AlwI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 2007, 2505
AlwNI CAGNNNCTG 4 cut(s) 1063, 1453, 1916, 1934
Ama87I CYCGRG 2 cut(s) 53, 2228
Aor51HI AGCGCT 2 cut(s) 1843, 1984
AoxI GGCC 5 cut(s) 7, 938, 1227, 1969, 2021
ApaLI GTGCAC 1 cut(s) 1896
ApoI RAATTY 5 cut(s) 190, 564, 1810, 2103, 2220
Asp700I GAANNNNTTC 1 cut(s) 1404
AspLEI GCGC 5 cut(s) 556, 1587, 1844, 1985, 2645
AspS9I GGNCC 8 cut(s) 295, 330, 939, 1496, 2021, 2201, 2285, 2424
AsuC2I CCSGG 3 cut(s) 2020, 2229, 2230
AsuHPI GGTGA 7 cut(s) 56, 127, 805, 1370, 1812, 1970, 2485
AvaI CYCGRG 2 cut(s) 53, 2228
AvaII GGWCC 6 cut(s) 295, 330, 1496, 2201, 2285, 2424
AxyI CCTNAGG 1 cut(s) 1605
BaeGI GKGCMC 1 cut(s) 1900
BanI GGYRCC 2 cut(s) 1584, 2671
BanII GRGCYC 3 cut(s) 60, 72, 201
BauI CACGAG 1 cut(s) 1752
BbsI GAAGAC 1 cut(s) 1766
Bbv12I GWGCWC 5 cut(s) 60, 72, 201, 1900, 2080
BccI CCATC 7 cut(s) 596, 844, 1000, 1151, 1289, 2144, 2378
BceAI ACGGC 2 cut(s) 308, 953
BciT130I CCWGG 8 cut(s) 254, 491, 1058, 1973, 2463, 2504, 2552, 2631
BclI TGATCA 2 cut(s) 225, 1644
BcnI CCSGG 3 cut(s) 2020, 2229, 2230
BcoDI GTCTC 7 cut(s) 151, 928, 1535, 1768, 2283, 2408, 2690
BcuI ACTAGT 1 cut(s) 1871
BfaI CTAG 3 cut(s) 684, 1307, 1872
BfmI CTRYAG 2 cut(s) 746, 2613
BfoI RGCGCY 3 cut(s) 1588, 1845, 1986
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 3 cut(s) 1438, 1831, 2296
BlpI GCTNAGC 1 cut(s) 882
BmcAI AGTACT 2 cut(s) 23, 820
Bme18I GGWCC 6 cut(s) 295, 330, 1496, 2201, 2285, 2424
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 2 cut(s) 53, 2228
BmgT120I GGNCC 8 cut(s) 295, 330, 939, 1496, 2021, 2201, 2285, 2424
BmiI GGNNCC 7 cut(s) 297, 855, 1586, 2203, 2214, 2426, 2673
BmsI GCATC 6 cut(s) 91, 380, 691, 1346, 2057, 2086
BpiI GAAGAC 1 cut(s) 1766
BpmI CTGGAG 1 cut(s) 1923
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 5 cut(s) 185, 485, 1208, 2265, 2432
BpuMI CCSGG 3 cut(s) 2020, 2229, 2230
Bsa29I ATCGAT 1 cut(s) 1340
BsaAI YACGTR 1 cut(s) 1216
BsaBI GATNNNNATC 3 cut(s) 405, 522, 1199
BsaHI GRCGYC 2 cut(s) 144, 1585
BsaI GGTCTC 1 cut(s) 1768
BsaJI CCNNGG 7 cut(s) 333, 1332, 2228, 2379, 2461, 2629, 2630
BsaXI ACNNNNNCTCC 2 cut(s) 2008, 2038
Bsc4I CCNNNNNNNGG 5 cut(s) 802, 1493, 2150, 2257, 2668
Bse1I ACTGG 7 cut(s) 675, 816, 1441, 1640, 1855, 1940, 2059
Bse21I CCTNAGG 1 cut(s) 1605
Bse3DI GCAATG 4 cut(s) 132, 1701, 2027, 2485
Bse8I GATNNNNATC 3 cut(s) 405, 522, 1199
BseBI CCWGG 8 cut(s) 254, 491, 1058, 1973, 2463, 2504, 2552, 2631
BseCI ATCGAT 1 cut(s) 1340
BseDI CCNNGG 7 cut(s) 333, 1332, 2228, 2379, 2461, 2629, 2630
BseGI GGATG 7 cut(s) 169, 406, 1624, 1856, 2101, 2389, 2560
BseJI GATNNNNATC 3 cut(s) 405, 522, 1199
BseLI CCNNNNNNNGG 5 cut(s) 802, 1493, 2150, 2257, 2668
BseMI GCAATG 4 cut(s) 132, 1701, 2027, 2485
BseMII CTCAG 7 cut(s) 456, 873, 879, 1550, 1596, 1623, 2093
BseNI ACTGG 7 cut(s) 675, 816, 1441, 1640, 1855, 1940, 2059
BseRI GAGGAG 2 cut(s) 846, 1802
BseSI GKGCMC 1 cut(s) 1900
BsgI GTGCAG 2 cut(s) 1416, 1902
BshFI GGCC 5 cut(s) 9, 940, 1229, 1971, 2023
BshNI GGYRCC 2 cut(s) 1584, 2671
BshVI ATCGAT 1 cut(s) 1340
BsiHKAI GWGCWC 5 cut(s) 60, 72, 201, 1900, 2080
BsiHKCI CYCGRG 2 cut(s) 53, 2228
BsiSI CCGG 2 cut(s) 2019, 2229
BslFI GGGAC 7 cut(s) 669, 1976, 2126, 2187, 2245, 2257, 2410
BslI CCNNNNNNNGG 5 cut(s) 802, 1493, 2150, 2257, 2668
BsmAI GTCTC 7 cut(s) 151, 928, 1535, 1768, 2283, 2408, 2690
BsmBI CGTCTC 2 cut(s) 151, 928
BsmFI GGGAC 7 cut(s) 669, 1976, 2126, 2187, 2245, 2257, 2410
BsmI GAATGC 2 cut(s) 342, 647
BsnI GGCC 5 cut(s) 9, 940, 1229, 1971, 2023
Bso31I GGTCTC 1 cut(s) 1768
BsoBI CYCGRG 2 cut(s) 53, 2228
Bsp1286I GDGCHC 5 cut(s) 60, 72, 201, 1900, 2080
Bsp1720I GCTNAGC 1 cut(s) 882
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 4 cut(s) 6, 104, 1367, 2192
BspANI GGCC 5 cut(s) 9, 940, 1229, 1971, 2023
BspCNI CTCAG 7 cut(s) 457, 874, 880, 1551, 1597, 1624, 2092
BspDI ATCGAT 1 cut(s) 1340
BspHI TCATGA 2 cut(s) 955, 2157
BspLI GGNNCC 7 cut(s) 297, 855, 1586, 2203, 2214, 2426, 2673
BspMI ACCTGC 3 cut(s) 1438, 1831, 2296
BspPI GGATC 7 cut(s) 371, 401, 784, 983, 1595, 2007, 2505
BspQI GCTCTTC 2 cut(s) 60, 2702
BspT107I GGYRCC 2 cut(s) 1584, 2671
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 1768
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 132, 1701, 2027, 2485
BsrI ACTGG 7 cut(s) 675, 816, 1441, 1640, 1855, 1940, 2059
BssECI CCNNGG 7 cut(s) 333, 1332, 2228, 2379, 2461, 2629, 2630
BssNI GRCGYC 2 cut(s) 144, 1585
BssSI CACGAG 1 cut(s) 1752
BssT1I CCWWGG 2 cut(s) 333, 1332
Bst2BI CACGAG 1 cut(s) 1752
Bst2UI CCWGG 8 cut(s) 254, 491, 1058, 1973, 2463, 2504, 2552, 2631
Bst4CI ACNGT 8 cut(s) 133, 761, 1170, 1180, 1465, 1537, 2114, 2617
Bst6I CTCTTC 2 cut(s) 60, 2702
BstACI GRCGYC 2 cut(s) 144, 1585
BstAFI CTTAAG 1 cut(s) 395
BstAPI GCANNNNNTGC 2 cut(s) 263, 1703
BstBAI YACGTR 1 cut(s) 1216
BstC8I GCNNGC 7 cut(s) 179, 245, 558, 753, 1035, 2007, 2400
BstDEI CTNAG 8 cut(s) 465, 882, 888, 1559, 1605, 1632, 2038, 2079
BstDSI CCRYGG 1 cut(s) 333
BstENI CCTNNNNNAGG 1 cut(s) 1491
BstF5I GGATG 7 cut(s) 169, 406, 1624, 1856, 2101, 2389, 2560
BstH2I RGCGCY 3 cut(s) 1588, 1845, 1986
BstHHI GCGC 5 cut(s) 556, 1587, 1844, 1985, 2645
BstMAI GTCTC 7 cut(s) 151, 928, 1535, 1768, 2283, 2408, 2690
BstNI CCWGG 8 cut(s) 254, 491, 1058, 1973, 2463, 2504, 2552, 2631
BstNSI RCATGY 4 cut(s) 181, 755, 1439, 2521
BstSFI CTRYAG 2 cut(s) 746, 2613
BstSLI GKGCMC 1 cut(s) 1900
BstSNI TACGTA 1 cut(s) 1216
BstV2I GAAGAC 1 cut(s) 1766
Bsu15I ATCGAT 1 cut(s) 1340
Bsu36I CCTNAGG 1 cut(s) 1605
BsuRI GGCC 5 cut(s) 9, 940, 1229, 1971, 2023
BsuTUI ATCGAT 1 cut(s) 1340
BtgI CCRYGG 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 1270
BtsCI GGATG 7 cut(s) 169, 406, 1624, 1856, 2101, 2389, 2560
BtsI GCAGTG 1 cut(s) 2360
BtsIMutI CAGTG 5 cut(s) 1647, 1654, 1947, 2080, 2360
BveI ACCTGC 3 cut(s) 1438, 1831, 2296
Cac8I GCNNGC 7 cut(s) 179, 245, 558, 753, 1035, 2007, 2400
CaiI CAGNNNCTG 4 cut(s) 1063, 1453, 1916, 1934
CciI TCATGA 2 cut(s) 955, 2157
CfoI GCGC 5 cut(s) 556, 1587, 1844, 1985, 2645
Cfr13I GGNCC 8 cut(s) 295, 330, 939, 1496, 2021, 2201, 2285, 2424
Cfr9I CCCGGG 1 cut(s) 2228
ClaI ATCGAT 1 cut(s) 1340
CseI GACGC 1 cut(s) 683
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 6 cut(s) 22, 819, 929, 982, 2042, 2341
CspCI CAANNNNNGTGG 6 cut(s) 528, 563, 931, 966, 2318, 2353
CviAII CATG 8 cut(s) 178, 334, 752, 956, 1436, 2158, 2418, 2518
CviQI GTAC 6 cut(s) 22, 819, 929, 982, 2042, 2341
DdeI CTNAG 8 cut(s) 465, 882, 888, 1559, 1605, 1632, 2038, 2079
DinI GGCGCC 1 cut(s) 1586
DraI TTTAAA 1 cut(s) 1075
DraIII CACNNNGTG 1 cut(s) 651
DrdI GACNNNNNNGTC 1 cut(s) 2422
DriI GACNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 2422
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 2 cut(s) 60, 2702
Eam1105I GACNNNNNGTC 1 cut(s) 148
EarI CTCTTC 2 cut(s) 60, 2702
Ecl136II GAGCTC 3 cut(s) 58, 70, 199
Eco105I TACGTA 1 cut(s) 1216
Eco130I CCWWGG 2 cut(s) 333, 1332
Eco24I GRGCYC 3 cut(s) 60, 72, 201
Eco31I GGTCTC 1 cut(s) 1768
Eco32I GATATC 1 cut(s) 1262
Eco47I GGWCC 6 cut(s) 295, 330, 1496, 2201, 2285, 2424
Eco47III AGCGCT 2 cut(s) 1843, 1984
Eco53kI GAGCTC 3 cut(s) 58, 70, 199
Eco57I CTGAAG 7 cut(s) 368, 1023, 1110, 1443, 1800, 1911, 2192
Eco81I CCTNAGG 1 cut(s) 1605
Eco88I CYCGRG 2 cut(s) 53, 2228
EcoICRI GAGCTC 3 cut(s) 58, 70, 199
EcoNI CCTNNNNNAGG 1 cut(s) 1491
EcoO109I RGGNCCY 3 cut(s) 295, 2285, 2424
EcoRII CCWGG 8 cut(s) 252, 489, 1056, 1971, 2461, 2502, 2550, 2629
EcoRV GATATC 1 cut(s) 1262
EcoT14I CCWWGG 2 cut(s) 333, 1332
EcoT38I GRGCYC 3 cut(s) 60, 72, 201
EgeI GGCGCC 1 cut(s) 1586
EheI GGCGCC 1 cut(s) 1586
ErhI CCWWGG 2 cut(s) 333, 1332
Esp3I CGTCTC 2 cut(s) 151, 928
FaeI CATG 8 cut(s) 181, 337, 755, 959, 1439, 2161, 2421, 2521
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 7 cut(s) 669, 1976, 2126, 2187, 2245, 2257, 2410
FatI CATG 8 cut(s) 177, 333, 751, 955, 1435, 2157, 2417, 2517
FauNDI CATATG 3 cut(s) 412, 434, 1891
FbaI TGATCA 2 cut(s) 225, 1644
FokI GGATG 7 cut(s) 176, 413, 1631, 1843, 2108, 2396, 2567
FriOI GRGCYC 3 cut(s) 60, 72, 201
FspBI CTAG 3 cut(s) 684, 1307, 1872
FspI TGCGCA 1 cut(s) 2644
GlaI GCGC 5 cut(s) 555, 1586, 1843, 1984, 2644
GsuI CTGGAG 1 cut(s) 1923
HaeII RGCGCY 3 cut(s) 1588, 1845, 1986
HaeIII GGCC 5 cut(s) 9, 940, 1229, 1971, 2023
HapII CCGG 2 cut(s) 2019, 2229
HgaI GACGC 1 cut(s) 683
HhaI GCGC 5 cut(s) 556, 1587, 1844, 1985, 2645
Hin1I GRCGYC 2 cut(s) 144, 1585
Hin1II CATG 8 cut(s) 181, 337, 755, 959, 1439, 2161, 2421, 2521
Hin6I GCGC 5 cut(s) 554, 1585, 1842, 1983, 2643
HinP1I GCGC 5 cut(s) 554, 1585, 1842, 1983, 2643
HincII GTYRAC 1 cut(s) 2085
HindII GTYRAC 1 cut(s) 2085
HindIII AAGCTT 2 cut(s) 1031, 1402
HpaII CCGG 2 cut(s) 2019, 2229
HphI GGTGA 7 cut(s) 56, 127, 805, 1370, 1812, 1970, 2485
Hpy166II GTNNAC 3 cut(s) 1898, 2066, 2085
Hpy8I GTNNAC 3 cut(s) 1898, 2066, 2085
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 8 cut(s) 34, 106, 133, 392, 1487, 1598, 2216, 2438
HpyCH4III ACNGT 8 cut(s) 133, 761, 1170, 1180, 1465, 1537, 2114, 2617
HpyCH4IV ACGT 2 cut(s) 144, 1215
HpyF3I CTNAG 8 cut(s) 465, 882, 888, 1559, 1605, 1632, 2038, 2079
HpySE526I ACGT 2 cut(s) 144, 1215
Hsp92I GRCGYC 2 cut(s) 144, 1585
Hsp92II CATG 8 cut(s) 181, 337, 755, 959, 1439, 2161, 2421, 2521
HspAI GCGC 5 cut(s) 554, 1585, 1842, 1983, 2643
KasI GGCGCC 1 cut(s) 1584
Ksp22I TGATCA 2 cut(s) 225, 1644
LguI GCTCTTC 2 cut(s) 60, 2702
LmnI GCTCC 6 cut(s) 196, 627, 859, 1942, 2017, 2395
LweI GCATC 6 cut(s) 91, 380, 691, 1346, 2057, 2086
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 3 cut(s) 684, 1307, 1872
MaeII ACGT 2 cut(s) 144, 1215
MaeIII GTNAC 5 cut(s) 733, 865, 1649, 1747, 2260
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 7 cut(s) 77, 796, 1269, 1436, 1771, 1793, 2362
MfeI CAATTG 1 cut(s) 172
MhlI GDGCHC 5 cut(s) 60, 72, 201, 1900, 2080
Mly113I GGCGCC 1 cut(s) 1585
MlyI GAGTC 5 cut(s) 8, 2239, 2647, 2675, 2692
MmeI TCCRAC 2 cut(s) 820, 1047
MroXI GAANNNNTTC 1 cut(s) 1404
MseI TTAA 6 cut(s) 108, 396, 963, 1074, 1197, 1479
MspA1I CMGCKG 3 cut(s) 779, 1163, 1931
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 2 cut(s) 2019, 2229
MunI CAATTG 1 cut(s) 172
Mva1269I GAATGC 2 cut(s) 342, 647
MvaI CCWGG 8 cut(s) 254, 491, 1058, 1973, 2463, 2504, 2552, 2631
NarI GGCGCC 1 cut(s) 1585
NciI CCSGG 3 cut(s) 2020, 2229, 2230
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 3 cut(s) 412, 434, 1891
NlaIII CATG 8 cut(s) 181, 337, 755, 959, 1439, 2161, 2421, 2521
NlaIV GGNNCC 7 cut(s) 297, 855, 1586, 2203, 2214, 2426, 2673
NmuCI GTSAC 2 cut(s) 865, 1649
NsbI TGCGCA 1 cut(s) 2644
NspI RCATGY 4 cut(s) 181, 755, 1439, 2521
PaeI GCATGC 2 cut(s) 181, 755
PagI TCATGA 2 cut(s) 955, 2157
PaqCI CACCTGC 1 cut(s) 1831
PasI CCCWGGG 1 cut(s) 2630
PciI ACATGT 2 cut(s) 1435, 2517
PciSI GCTCTTC 2 cut(s) 60, 2702
PctI GAATGC 2 cut(s) 342, 647
PdmI GAANNNNTTC 1 cut(s) 1404
PflMI CCANNNNNTGG 2 cut(s) 802, 2150
PfoI TCCNGGA 1 cut(s) 489
PleI GAGTC 5 cut(s) 8, 2239, 2647, 2674, 2692
PluTI GGCGCC 1 cut(s) 1588
PpsI GAGTC 5 cut(s) 8, 2239, 2647, 2674, 2692
Ppu21I YACGTR 1 cut(s) 1216
PpuMI RGGWCCY 3 cut(s) 295, 2285, 2424
PscI ACATGT 2 cut(s) 1435, 2517
Psp124BI GAGCTC 3 cut(s) 60, 72, 201
Psp5II RGGWCCY 3 cut(s) 295, 2285, 2424
Psp6I CCWGG 8 cut(s) 252, 489, 1056, 1971, 2461, 2502, 2550, 2629
PspGI CCWGG 8 cut(s) 252, 489, 1056, 1971, 2461, 2502, 2550, 2629
PspN4I GGNNCC 7 cut(s) 297, 855, 1586, 2203, 2214, 2426, 2673
PspPI GGNCC 8 cut(s) 295, 330, 939, 1496, 2021, 2201, 2285, 2424
PspPPI RGGWCCY 3 cut(s) 295, 2285, 2424
PstNI CAGNNNCTG 4 cut(s) 1063, 1453, 1916, 1934
PvuII CAGCTG 3 cut(s) 779, 1163, 1931
RsaI GTAC 6 cut(s) 23, 820, 930, 983, 2043, 2342
RsaNI GTAC 6 cut(s) 22, 819, 929, 982, 2042, 2341
SacI GAGCTC 3 cut(s) 60, 72, 201
SapI GCTCTTC 2 cut(s) 60, 2702
SaqAI TTAA 6 cut(s) 108, 396, 963, 1074, 1197, 1479
Sau96I GGNCC 8 cut(s) 295, 330, 939, 1496, 2021, 2201, 2285, 2424
ScaI AGTACT 2 cut(s) 23, 820
SchI GAGTC 5 cut(s) 8, 2239, 2647, 2675, 2692
SduI GDGCHC 5 cut(s) 60, 72, 201, 1900, 2080
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 6 cut(s) 91, 380, 691, 1346, 2057, 2086
SfcI CTRYAG 2 cut(s) 746, 2613
SfoI GGCGCC 1 cut(s) 1586
SinI GGWCC 6 cut(s) 295, 330, 1496, 2201, 2285, 2424
SmaI CCCGGG 1 cut(s) 2230
SmlI CTYRAG 6 cut(s) 200, 395, 500, 1187, 2280, 2411
SmoI CTYRAG 6 cut(s) 200, 395, 500, 1187, 2280, 2411
SnaBI TACGTA 1 cut(s) 1216
SpeI ACTAGT 1 cut(s) 1871
SphI GCATGC 2 cut(s) 181, 755
SsiI CCGC 4 cut(s) 6, 104, 1367, 2192
SspDI GGCGCC 1 cut(s) 1584
SspI AATATT 1 cut(s) 368
SspMI CTAG 3 cut(s) 684, 1307, 1872
SstI GAGCTC 3 cut(s) 60, 72, 201
StyI CCWWGG 2 cut(s) 333, 1332
TaaI ACNGT 8 cut(s) 133, 761, 1170, 1180, 1465, 1537, 2114, 2617
TaiI ACGT 2 cut(s) 147, 1218
TaqI TCGA 2 cut(s) 1340, 1772
TatI WGTACW 4 cut(s) 21, 818, 928, 981
TauI GCSGC 2 cut(s) 9, 2195
Tru1I TTAA 6 cut(s) 108, 396, 963, 1074, 1197, 1479
Tru9I TTAA 6 cut(s) 108, 396, 963, 1074, 1197, 1479
TscAI CASTG 5 cut(s) 1647, 1654, 1947, 2080, 2367
TseFI GTSAC 2 cut(s) 865, 1649
Tsp45I GTSAC 2 cut(s) 865, 1649
TspDTI ATGAA 7 cut(s) 654, 972, 1290, 1335, 1701, 1845, 2146
TspGWI ACGGA 2 cut(s) 176, 1112
TspMI CCCGGG 1 cut(s) 2228
TspRI CASTG 5 cut(s) 1647, 1654, 1947, 2080, 2367
Van91I CCANNNNNTGG 2 cut(s) 802, 2150
Vha464I CTTAAG 1 cut(s) 395
VneI GTGCAC 1 cut(s) 1896
VpaK11BI GGWCC 6 cut(s) 295, 330, 1496, 2201, 2285, 2424
XagI CCTNNNNNAGG 1 cut(s) 1491
XapI RAATTY 5 cut(s) 190, 564, 1810, 2103, 2220
XbaI TCTAGA 1 cut(s) 683
XceI RCATGY 4 cut(s) 181, 755, 1439, 2521
XmaI CCCGGG 1 cut(s) 2228
XmnI GAANNNNTTC 1 cut(s) 1404
XspI CTAG 3 cut(s) 684, 1307, 1872
ZraI GACGTC 1 cut(s) 145
ZrmI AGTACT 2 cut(s) 23, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.