AT4G25190

QWRF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010964
Physical Location & Seq
Reverse (-)
12914183 .. 12916156
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25190.2

Sequence Viewer

Length: 1185 bp
ATGGCAACCACCGGTCGGAGACTCCGTCCTCCATCGCCCAATAATAACCGGAGCCGGACTATAAGCTCTTCAATCTCTCTCCCAGTGTCCTTAAACGCCTCGTTATCGTCTTCTACTTCTTCATCTTCTTCCTCTTCGCCTTCCAATTCAAGCAAACGAGTGATGATAACCAGATCACAGTCCACAACAAGATCCTCCAGACCCATCGGATCATCCGACTCTAAATCCGGCGAGAACATAATCCCGGCGAGAAACAGCGCATCAAGATCTCAAGAGATAAACAACGGTAGAAGCAGAGAGTCGTTTGCTCGGTACTTGGAGCAACGTACGCGTGGTAGTCCTCGTAGTAACGCTTCATCGAGAGGGGTAAAGCCGGGAGCCAGCTCACCATCGGCGTGGGCGTTGTCTCCTGGAAGACTTTCGACTATGAAGACGCCTCTGTCCAGCTCAGCTCCGACGACTTCAATGTGCATGACGCCGCCTGAGTCACCCGTAAGCAAGGCCAAGATAAGAAGCGGTGGCGGTGGAGCGGTGGCAGGAGTTTTGAAGTACTTCATGGCGCAGAAGAAAGTATCTCCGGTACAAGAGGAGGATTATCACCGGTTTAGGATTTTTCAAAACAGATTACTTCAATGGAGGTTTGTTAACGCAAGAACTGAAGCCACTATGGCAAACCTTAAGATCAACGTTGAGGATCAGTTGTTTTGGGTTTGGCTTAGGATATACAAAATGAGGAACTACGTTGTAGAAAATTTGATTGAAATTCAAAGGCTCCGTCAAGATATCAAAGTACGTGAAGTTCTCAGCCTCCAAATGCCTTTACTTAATGAGTGGTCAAAAATAGACGCAAAAAACTCCGAAGCGTTGAGCAAGCTAACTCGAAAACTCCATGCTTTGTCTGTCCGATTACCCCTCGTACATGGTGCCACGATAGATATGGTGTCGATACATGAAGAAATGGTCATAGCTATAGAAGTCATGGACGAGATCGAAGACGTCATCATCAAGTTTCTCCCACGAGTTGAAATAATCCTCTATGAGCTGACAGAATTGATCGGCATGTTCAATCAAGAATTGTTATATTTTGAAGAAATGGACGAGAGCCTTTTATCCATTCCTCTCTTCACGGCAAAGGAGAGTAGCTTGAGGGTCCATATTCTCCAAAAGACTGAAGAACAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

394

Amino Acids

44.22

Weight (kDa)

10.05

Isoelectric Point (pI)

73.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 38 - 360 1.4e-87 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 439
AatII GACGTC 1 cut(s) 999
AccB1I GGYRCC 1 cut(s) 923
AccBSI CCGCTC 1 cut(s) 530
AccII CGCG 1 cut(s) 331
AciI CCGC 4 cut(s) 479, 516, 522, 530
AclI AACGTT 1 cut(s) 687
AclWI GGATC 3 cut(s) 186, 217, 702
AcsI RAATTY 2 cut(s) 751, 762
AcuI CTGAAG 1 cut(s) 678
AcyI GRCGYC 3 cut(s) 434, 476, 996
AfaI GTAC 6 cut(s) 314, 328, 551, 582, 792, 918
AfiI CCNNNNNNNGG 1 cut(s) 15
AflII CTTAAG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 329
AgeI ACCGGT 2 cut(s) 11, 600
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 8 cut(s) 66, 384, 447, 452, 874, 968, 1042, 1143
AluI AGCT 8 cut(s) 66, 384, 447, 452, 874, 968, 1042, 1143
Alw26I GTCTC 2 cut(s) 13, 411
AlwI GGATC 3 cut(s) 186, 217, 702
AoxI GGCC 1 cut(s) 501
ApoI RAATTY 2 cut(s) 751, 762
ArsI GACNNNNNNTTYG 2 cut(s) 760, 792
AsiGI ACCGGT 2 cut(s) 11, 600
Asp700I GAANNNNTTC 2 cut(s) 418, 551
AspLEI GCGC 2 cut(s) 260, 562
AspS9I GGNCC 1 cut(s) 1150
AsuC2I CCSGG 2 cut(s) 245, 375
AsuHPI GGTGA 3 cut(s) 378, 480, 590
AvaII GGWCC 1 cut(s) 1150
BanI GGYRCC 1 cut(s) 923
BarI GAAGNNNNNNTAC 2 cut(s) 337, 369
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 4 cut(s) 102, 421, 437, 999
BccI CCATC 3 cut(s) 40, 212, 397
BceAI ACGGC 1 cut(s) 1143
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 2 cut(s) 245, 375
BcoDI GTCTC 2 cut(s) 13, 411
BfmI CTRYAG 1 cut(s) 969
BfrI CTTAAG 1 cut(s) 677
BglI GCCNNNNNGGC 1 cut(s) 668
BglII AGATCT 1 cut(s) 266
BisI GCNGC 1 cut(s) 479
BlpI GCTNAGC 1 cut(s) 448
BlsI GCNGC 1 cut(s) 480
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 3 cut(s) 245, 375, 411
Bme18I GGWCC 1 cut(s) 1150
BmgT120I GGNCC 1 cut(s) 1150
BmiI GGNNCC 5 cut(s) 53, 379, 773, 925, 1151
BmrFI CCNGG 3 cut(s) 245, 375, 411
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 77
BpiI GAAGAC 4 cut(s) 102, 421, 437, 999
BpmI CTGGAG 1 cut(s) 181
Bpu10I CCTNAGC 1 cut(s) 716
Bpu1102I GCTNAGC 1 cut(s) 448
BpuEI CTTGAG 2 cut(s) 255, 1165
BpuMI CCSGG 2 cut(s) 245, 375
BsaAI YACGTR 1 cut(s) 794
BsaHI GRCGYC 3 cut(s) 434, 476, 996
BsaWI WCCGGW 4 cut(s) 11, 48, 577, 600
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 2 cut(s) 11, 600
Bse1I ACTGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 411
BseGI GGATG 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 462, 474, 817
BseNI ACTGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 503
BshNI GGYRCC 1 cut(s) 923
BshTI ACCGGT 2 cut(s) 11, 600
BsiEI CGRYCG 1 cut(s) 16
BsiSI CCGG 8 cut(s) 12, 49, 55, 228, 245, 374, 578, 601
BsiWI CGTACG 1 cut(s) 326
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 13, 411
BsnI GGCC 1 cut(s) 503
Bsp143I GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
Bsp1720I GCTNAGC 1 cut(s) 448
BspACI CCGC 4 cut(s) 479, 516, 522, 530
BspANI GGCC 1 cut(s) 503
BspCNI CTCAG 3 cut(s) 461, 475, 816
BspFNI CGCG 1 cut(s) 331
BspLI GGNNCC 5 cut(s) 53, 379, 773, 925, 1151
BspPI GGATC 3 cut(s) 186, 217, 702
BspQI GCTCTTC 1 cut(s) 73
BspT107I GGYRCC 1 cut(s) 923
BspTI CTTAAG 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 530
BsrFI RCCGGY 2 cut(s) 11, 600
BsrI ACTGG 1 cut(s) 83
BssAI RCCGGY 2 cut(s) 11, 600
BssMI GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
BssNI GRCGYC 3 cut(s) 434, 476, 996
BssSI CACGAG 1 cut(s) 1017
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 180, 287
Bst6I CTCTTC 3 cut(s) 73, 139, 1127
BstACI GRCGYC 3 cut(s) 434, 476, 996
BstAFI CTTAAG 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 794
BstC8I GCNNGC 2 cut(s) 382, 872
BstDEI CTNAG 4 cut(s) 448, 483, 716, 803
BstF5I GGATG 1 cut(s) 212
BstFNI CGCG 1 cut(s) 331
BstHHI GCGC 2 cut(s) 260, 562
BstKTI GATC 8 cut(s) 176, 194, 212, 269, 684, 697, 990, 1056
BstMAI GTCTC 2 cut(s) 13, 411
BstMBI GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 328, 668
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 1063
BstSCI CCNGG 3 cut(s) 243, 373, 409
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 1 cut(s) 331
BstV2I GAAGAC 4 cut(s) 102, 421, 437, 999
BstX2I RGATCY 2 cut(s) 191, 266
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 2 cut(s) 191, 266
BsuRI GGCC 1 cut(s) 503
BtgZI GCGATG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 212
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 382, 872
CfoI GCGC 2 cut(s) 260, 562
Cfr10I RCCGGY 2 cut(s) 11, 600
Cfr13I GGNCC 1 cut(s) 1150
CseI GACGC 3 cut(s) 442, 484, 854
Csp6I GTAC 6 cut(s) 313, 327, 550, 581, 791, 917
CspAI ACCGGT 2 cut(s) 11, 600
CviAII CATG 7 cut(s) 472, 556, 890, 920, 950, 979, 1060
CviQI GTAC 6 cut(s) 313, 327, 550, 581, 791, 917
DdeI CTNAG 4 cut(s) 448, 483, 716, 803
DpnI GATC 8 cut(s) 175, 193, 211, 268, 683, 696, 989, 1055
DpnII GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
DrdI GACNNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 439
Eam1104I CTCTTC 3 cut(s) 73, 139, 1127
EarI CTCTTC 3 cut(s) 73, 139, 1127
Eco32I GATATC 1 cut(s) 784
Eco47I GGWCC 1 cut(s) 1150
Eco57I CTGAAG 1 cut(s) 678
EcoRII CCWGG 1 cut(s) 409
EcoRV GATATC 1 cut(s) 784
FaeI CATG 7 cut(s) 475, 559, 893, 923, 953, 982, 1063
FatI CATG 7 cut(s) 471, 555, 889, 919, 949, 978, 1059
Fnu4HI GCNGC 1 cut(s) 479
FokI GGATG 1 cut(s) 199
Fsp4HI GCNGC 1 cut(s) 479
GlaI GCGC 2 cut(s) 259, 561
GluI GCNGC 1 cut(s) 479
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 1 cut(s) 503
HapII CCGG 8 cut(s) 12, 49, 55, 228, 245, 374, 578, 601
HgaI GACGC 3 cut(s) 442, 484, 854
HhaI GCGC 2 cut(s) 260, 562
Hin1I GRCGYC 3 cut(s) 434, 476, 996
Hin1II CATG 7 cut(s) 475, 559, 893, 923, 953, 982, 1063
Hin6I GCGC 2 cut(s) 258, 560
HinP1I GCGC 2 cut(s) 258, 560
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 4 cut(s) 21, 218, 299, 485
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 8 cut(s) 12, 49, 55, 228, 245, 374, 578, 601
HphI GGTGA 3 cut(s) 378, 480, 590
Hpy166II GTNNAC 2 cut(s) 183, 646
Hpy188I TCNGA 6 cut(s) 18, 209, 217, 456, 859, 905
Hpy188III TCNNGA 6 cut(s) 198, 264, 272, 360, 779, 1070
Hpy8I GTNNAC 2 cut(s) 183, 646
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 150
HpyCH4III ACNGT 2 cut(s) 180, 287
HpyCH4IV ACGT 5 cut(s) 325, 687, 741, 793, 996
HpyCH4V TGCA 1 cut(s) 471
HpyF10VI GCNNNNNNNGC 2 cut(s) 328, 668
HpyF3I CTNAG 4 cut(s) 448, 483, 716, 803
HpySE526I ACGT 5 cut(s) 325, 687, 741, 793, 996
Hsp92I GRCGYC 3 cut(s) 434, 476, 996
Hsp92II CATG 7 cut(s) 475, 559, 893, 923, 953, 982, 1063
HspAI GCGC 2 cut(s) 258, 560
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
LguI GCTCTTC 1 cut(s) 73
LmnI GCTCC 6 cut(s) 51, 319, 377, 457, 527, 777
LweI GCATC 1 cut(s) 269
MaeII ACGT 5 cut(s) 325, 687, 741, 793, 996
MaeIII GTNAC 2 cut(s) 347, 486
MalI GATC 8 cut(s) 175, 193, 211, 268, 683, 696, 989, 1055
MbiI CCGCTC 1 cut(s) 530
MboI GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
MflI RGATCY 2 cut(s) 191, 266
MluCI AATT 5 cut(s) 145, 751, 762, 1049, 1073
MluI ACGCGT 1 cut(s) 329
MlyI GAGTC 4 cut(s) 15, 212, 308, 494
MmeI TCCRAC 2 cut(s) 240, 479
MroXI GAANNNNTTC 2 cut(s) 418, 551
MseI TTAA 4 cut(s) 92, 645, 678, 825
MslI CAYNNNNRTG 1 cut(s) 394
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 8 cut(s) 12, 49, 55, 228, 245, 374, 578, 601
MspR9I CCNGG 3 cut(s) 245, 375, 411
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 2 cut(s) 328, 668
NciI CCSGG 2 cut(s) 245, 375
NdeII GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
NlaIII CATG 7 cut(s) 475, 559, 893, 923, 953, 982, 1063
NlaIV GGNNCC 5 cut(s) 53, 379, 773, 925, 1151
NmuCI GTSAC 1 cut(s) 486
NspI RCATGY 1 cut(s) 1063
PciSI GCTCTTC 1 cut(s) 73
PcsI WCGNNNNNNNCGW 3 cut(s) 22, 213, 398
PdmI GAANNNNTTC 2 cut(s) 418, 551
Pfl23II CGTACG 1 cut(s) 326
PflFI GACNNNGTC 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 409
PinAI ACCGGT 2 cut(s) 11, 600
PkrI GCNGC 1 cut(s) 480
PleI GAGTC 4 cut(s) 15, 212, 307, 493
PpsI GAGTC 4 cut(s) 15, 212, 307, 493
Ppu21I YACGTR 1 cut(s) 794
Psp1406I AACGTT 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspLI CGTACG 1 cut(s) 326
PspN4I GGNNCC 5 cut(s) 53, 379, 773, 925, 1151
PspPI GGNCC 1 cut(s) 1150
PsrI GAACNNNNNNTAC 2 cut(s) 783, 815
PsuI RGATCY 2 cut(s) 191, 266
PsyI GACNNNGTC 1 cut(s) 24
RsaI GTAC 6 cut(s) 314, 328, 551, 582, 792, 918
RsaNI GTAC 6 cut(s) 313, 327, 550, 581, 791, 917
RseI CAYNNNNRTG 1 cut(s) 394
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 4 cut(s) 92, 645, 678, 825
SatI GCNGC 1 cut(s) 479
Sau3AI GATC 8 cut(s) 173, 191, 209, 266, 681, 694, 987, 1053
Sau96I GGNCC 1 cut(s) 1150
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 4 cut(s) 15, 212, 308, 494
ScrFI CCNGG 3 cut(s) 245, 375, 411
SfaNI GCATC 1 cut(s) 269
SfcI CTRYAG 1 cut(s) 969
SinI GGWCC 1 cut(s) 1150
SmiMI CAYNNNNRTG 1 cut(s) 394
SmlI CTYRAG 3 cut(s) 270, 677, 1144
SmoI CTYRAG 3 cut(s) 270, 677, 1144
Sse9I AATT 5 cut(s) 145, 751, 762, 1049, 1073
SsiI CCGC 4 cut(s) 479, 516, 522, 530
StyD4I CCNGG 3 cut(s) 243, 373, 409
TaaI ACNGT 2 cut(s) 180, 287
TaiI ACGT 5 cut(s) 328, 690, 744, 796, 999
TaqI TCGA 5 cut(s) 359, 422, 880, 944, 990
TasI AATT 5 cut(s) 145, 751, 762, 1049, 1073
TatI WGTACW 1 cut(s) 549
TauI GCSGC 1 cut(s) 481
Tru1I TTAA 4 cut(s) 92, 645, 678, 825
Tru9I TTAA 4 cut(s) 92, 645, 678, 825
TscAI CASTG 1 cut(s) 90
TseFI GTSAC 1 cut(s) 486
Tsp45I GTSAC 1 cut(s) 486
TspDTI ATGAA 5 cut(s) 111, 345, 443, 544, 966
TspGWI ACGGA 2 cut(s) 14, 764
TspRI CASTG 1 cut(s) 90
Tth111I GACNNNGTC 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 1150
XapI RAATTY 2 cut(s) 751, 762
XceI RCATGY 1 cut(s) 1063
XcmI CCANNNNNNNNNTGG 1 cut(s) 934
XmnI GAANNNNTTC 2 cut(s) 418, 551
ZraI GACGTC 1 cut(s) 997
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.