pycom01g19170

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010964
Physical Location & Seq
Reverse (-)
17962825 .. 17966128
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g19170.1

Sequence Viewer

Length: 1152 bp
ATGAACAACACTTACATGTCACGCAGGCAGCAGCAGCAATCCAGCCCTCGACTTCTCAGAAGTAGAAGTGGAACTACTTCTCCGACCACAACCCTAACCCTAACCATACCGCAAAACATTTCAAGTTCATCATGCACTACCACGCCATGCAACAGCCAAAGATTCACCACCCCCTGTCGCTCGAAATCCATCGTAAAATCAAGGGGCAATAAGGATCAGAAGGAAGAGAAAGTACATGCACTCAAGCAAAAGAAGGGCGGCCAAGAAAGTATCAGTAGAAAACAAGGTACTAATAATTTAGGTTTAAATACAAGTTTTGCTAAGTTGCAGGGTGGGAGTACTAGTCCAAGTCCACGCCCTAGGATATCAGTGTCCGTGCCACCTCCGAGGTCTCCGTCCGCATGGGCGTTGTCCCCGGGACGGACTTTGCCGTGGAAGGTGGCGCCGGAGTCCCCGGCAGCTGTGCCTTCCACAAGAGCATCTAAGGTTAAGTCTAGTGGTGGCGGGGTTGGTGGGGTTTTGAAGTATTTTAGGCTGCAGAAGAAAGTGTCGCCGGTACAAGAGGAGGACTTCCACCGGTTTCGGCTTTTGCATAATAGGCTACTGCAGTGGAGGTTTGCAAATGCCAGAGCTGAGGCTGCTAAGATTGCTGCACAGAAAAGTTCACAGGATAAAATATTTAGTGTGTGGCTTAGAACATTGAAGGTGAGAAACTTCATCCTAGACAAAAGAATGCAAGTGCAAAAGCTTAAGCATGAGATTAAGGTGTACCAGATTTTGAACCCTCAAATTATTCTATTGAATGAATGGGGAAAACTGGATAAAAAAAATCAAGAATCCGTCAGCAGGTTGGTGAGAAAATTGTCTGGAATTTCAAACACAATCCCTTTGGTACATGATGCGGAGGCAGATGTGGAGGCTGTGTACGAAGCCATGACCACGGCCATGGCTATGATGGAAGGTATTTTAGGAATGGTCTCTAAACTGTTGCCGCAGCTGGAAAAGGTGCTTTACATGGTTACAGAGCTTTTAATTGCACAGAAACAACAGAGAGAATTGGAGAATAATTTAGCCTTGGTTTCCGCACTTGCGGTGAGTAACATAATTTCTTGCTGTCAAGCTCAACCATTTTTCTTAGTGATAAATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

384

Amino Acids

42.67

Weight (kDa)

10.4

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 52 - 352 7.2e-66 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 442
AciI CCGC 8 cut(s) 110, 258, 399, 504, 902, 992, 1083, 1091
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 2 cut(s) 259, 942
AcsI RAATTY 1 cut(s) 870
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 7 cut(s) 234, 289, 340, 558, 770, 894, 926
AfiI CCNNNNNNNGG 2 cut(s) 420, 846
AflII CTTAAG 1 cut(s) 749
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 576
AgsI TTSAA 6 cut(s) 123, 523, 703, 781, 802, 876
AhlI ACTAGT 1 cut(s) 341
AloI GAACNNNNNNTCC 2 cut(s) 64, 96
AluBI AGCT 6 cut(s) 461, 632, 748, 997, 1027, 1121
AluI AGCT 6 cut(s) 461, 632, 748, 997, 1027, 1121
Alw26I GTCTC 2 cut(s) 396, 982
AlwI GGATC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 415
AoxI GGCC 2 cut(s) 259, 942
ApeKI GCWGC 8 cut(s) 28, 31, 34, 458, 535, 638, 650, 994
ApoI RAATTY 1 cut(s) 870
AsiGI ACCGGT 1 cut(s) 576
Asp700I GAANNNNTTC 1 cut(s) 76
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 1 cut(s) 445
AsuC2I CCSGG 3 cut(s) 416, 417, 455
AsuHPI GGTGA 4 cut(s) 157, 718, 865, 1105
AvaI CYCGRG 1 cut(s) 415
AvrII CCTAGG 1 cut(s) 359
BanI GGYRCC 1 cut(s) 442
BarI GAAGNNNNNNTAC 4 cut(s) 58, 90, 216, 248
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 8 cut(s) 40, 43, 46, 470, 522, 625, 637, 1006
BccI CCATC 2 cut(s) 197, 949
BceAI ACGGC 2 cut(s) 415, 957
BcnI CCSGG 3 cut(s) 416, 417, 455
BcoDI GTCTC 2 cut(s) 396, 982
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 4 cut(s) 342, 360, 495, 722
BfmI CTRYAG 2 cut(s) 536, 605
BfoI RGCGCY 1 cut(s) 446
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 837
BlnI CCTAGG 1 cut(s) 359
BmcAI AGTACT 1 cut(s) 340
Bme1390I CCNGG 3 cut(s) 416, 417, 455
BmeT110I CYCGRG 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 444
BmrFI CCNGG 3 cut(s) 416, 417, 455
BmsI GCATC 2 cut(s) 488, 889
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 3 cut(s) 416, 417, 455
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 2 cut(s) 396, 982
BsaJI CCNNGG 9 cut(s) 359, 386, 414, 415, 431, 453, 939, 945, 1074
BsaWI WCCGGW 1 cut(s) 576
BsaXI ACNNNNNCTCC 6 cut(s) 64, 94, 328, 358, 908, 938
Bsc4I CCNNNNNNNGG 2 cut(s) 420, 846
Bse118I RCCGGY 2 cut(s) 553, 576
Bse1I ACTGG 1 cut(s) 822
BseDI CCNNGG 9 cut(s) 359, 386, 414, 415, 431, 453, 939, 945, 1074
BseGI GGATG 1 cut(s) 717
BseLI CCNNNNNNNGG 2 cut(s) 420, 846
BseMII CTCAG 2 cut(s) 70, 624
BseNI ACTGG 1 cut(s) 822
BseRI GAGGAG 1 cut(s) 578
BseXI GCAGC 8 cut(s) 40, 43, 46, 470, 522, 625, 637, 1006
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 2 cut(s) 261, 944
BshNI GGYRCC 1 cut(s) 442
BshTI ACCGGT 1 cut(s) 576
BsiHKCI CYCGRG 1 cut(s) 415
BsiSI CCGG 5 cut(s) 416, 446, 455, 554, 577
BslFI GGGAC 3 cut(s) 397, 432, 436
BslI CCNNNNNNNGG 2 cut(s) 420, 846
BsmAI GTCTC 2 cut(s) 396, 982
BsmFI GGGAC 3 cut(s) 397, 432, 436
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 2 cut(s) 261, 944
Bso31I GGTCTC 2 cut(s) 396, 982
BsoBI CYCGRG 1 cut(s) 415
Bsp143I GATC 1 cut(s) 214
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 8 cut(s) 110, 258, 399, 504, 902, 992, 1083, 1091
BspANI GGCC 2 cut(s) 261, 944
BspCNI CTCAG 2 cut(s) 69, 625
BspLI GGNNCC 1 cut(s) 444
BspMAI CTGCAG 2 cut(s) 540, 609
BspMI ACCTGC 1 cut(s) 837
BspPI GGATC 1 cut(s) 222
BspT107I GGYRCC 1 cut(s) 442
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 2 cut(s) 396, 982
BsrFI RCCGGY 2 cut(s) 553, 576
BsrI ACTGG 1 cut(s) 822
BssAI RCCGGY 2 cut(s) 553, 576
BssECI CCNNGG 9 cut(s) 359, 386, 414, 415, 431, 453, 939, 945, 1074
BssMI GATC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 443
BssT1I CCWWGG 3 cut(s) 359, 945, 1074
Bst4CI ACNGT 1 cut(s) 987
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 749
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 7 cut(s) 56, 321, 483, 633, 642, 692, 1135
BstDSI CCRYGG 3 cut(s) 431, 939, 945
BstF5I GGATG 1 cut(s) 717
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 2 cut(s) 396, 982
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 4 cut(s) 34, 598, 638, 647
BstNSI RCATGY 2 cut(s) 19, 239
BstSCI CCNGG 3 cut(s) 414, 415, 453
BstSFI CTRYAG 2 cut(s) 536, 605
BstV1I GCAGC 8 cut(s) 40, 43, 46, 470, 522, 625, 637, 1006
BstXI CCANNNNNNTGG 1 cut(s) 946
BsuRI GGCC 2 cut(s) 261, 944
BtgI CCRYGG 3 cut(s) 431, 939, 945
BtsCI GGATG 1 cut(s) 717
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 2 cut(s) 375, 614
BveI ACCTGC 1 cut(s) 837
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 2 cut(s) 553, 576
Cfr9I CCCGGG 1 cut(s) 415
Csp6I GTAC 7 cut(s) 233, 288, 339, 557, 769, 893, 925
CspAI ACCGGT 1 cut(s) 576
CviQI GTAC 7 cut(s) 233, 288, 339, 557, 769, 893, 925
DdeI CTNAG 7 cut(s) 56, 321, 483, 633, 642, 692, 1135
DinI GGCGCC 1 cut(s) 444
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
DraI TTTAAA 1 cut(s) 306
EaeI YGGCCR 2 cut(s) 259, 942
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 3 cut(s) 359, 945, 1074
Eco31I GGTCTC 2 cut(s) 396, 982
Eco32I GATATC 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 415
EcoRV GATATC 1 cut(s) 366
EcoT14I CCWWGG 3 cut(s) 359, 945, 1074
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
ErhI CCWWGG 3 cut(s) 359, 945, 1074
FaqI GGGAC 3 cut(s) 397, 432, 436
FauI CCCGC 1 cut(s) 497
FokI GGATG 1 cut(s) 704
FspBI CTAG 4 cut(s) 342, 360, 495, 722
GlaI GCGC 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 2 cut(s) 261, 944
HapII CCGG 5 cut(s) 416, 446, 455, 554, 577
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 443
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 3 cut(s) 162, 449, 836
HpaII CCGG 5 cut(s) 416, 446, 455, 554, 577
HphI GGTGA 4 cut(s) 157, 718, 865, 1105
Hpy166II GTNNAC 4 cut(s) 353, 665, 769, 925
Hpy188I TCNGA 4 cut(s) 59, 84, 219, 387
Hpy188III TCNNGA 2 cut(s) 833, 867
Hpy8I GTNNAC 4 cut(s) 353, 665, 769, 925
HpyAV CCTTC 6 cut(s) 214, 247, 430, 477, 697, 953
HpyCH4III ACNGT 1 cut(s) 987
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 598, 638, 647
HpyF3I CTNAG 7 cut(s) 56, 321, 483, 633, 642, 692, 1135
Hsp92I GRCGYC 1 cut(s) 443
HspAI GCGC 1 cut(s) 443
KasI GGCGCC 1 cut(s) 442
Kzo9I GATC 1 cut(s) 214
Lsp1109I GCAGC 8 cut(s) 40, 43, 46, 470, 522, 625, 637, 1006
LweI GCATC 2 cut(s) 488, 889
MaeI CTAG 4 cut(s) 342, 360, 495, 722
MaeIII GTNAC 3 cut(s) 18, 1018, 1097
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 2 cut(s) 236, 553
MluCI AATT 8 cut(s) 295, 789, 860, 870, 1032, 1055, 1066, 1104
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 76
MseI TTAA 5 cut(s) 305, 489, 750, 762, 1031
MslI CAYNNNNRTG 3 cut(s) 14, 944, 950
MspA1I CMGCKG 2 cut(s) 461, 997
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 5 cut(s) 416, 446, 455, 554, 577
MspR9I CCNGG 3 cut(s) 416, 417, 455
Mva1269I GAATGC 1 cut(s) 738
MwoI GCNNNNNNNGC 4 cut(s) 34, 598, 638, 647
NarI GGCGCC 1 cut(s) 443
NciI CCSGG 3 cut(s) 416, 417, 455
NcoI CCATGG 1 cut(s) 945
NdeII GATC 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 444
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 2 cut(s) 19, 239
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 76
PfeI GAWTC 2 cut(s) 162, 836
PinAI ACCGGT 1 cut(s) 576
PleI GAGTC 1 cut(s) 457
PluTI GGCGCC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 457
PscI ACATGT 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 444
PsrI GAACNNNNNNTAC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 540, 609
PvuII CAGCTG 2 cut(s) 461, 997
RsaI GTAC 7 cut(s) 234, 289, 340, 558, 770, 894, 926
RsaNI GTAC 7 cut(s) 233, 288, 339, 557, 769, 893, 925
RseI CAYNNNNRTG 3 cut(s) 14, 944, 950
SaqAI TTAA 5 cut(s) 305, 489, 750, 762, 1031
Sau3AI GATC 1 cut(s) 214
ScaI AGTACT 1 cut(s) 340
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 416, 417, 455
SfaNI GCATC 2 cut(s) 488, 889
SfcI CTRYAG 2 cut(s) 536, 605
SfoI GGCGCC 1 cut(s) 444
SmaI CCCGGG 1 cut(s) 417
SmiMI CAYNNNNRTG 3 cut(s) 14, 944, 950
SmlI CTYRAG 2 cut(s) 242, 749
SmoI CTYRAG 2 cut(s) 242, 749
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 8 cut(s) 295, 789, 860, 870, 1032, 1055, 1066, 1104
SsiI CCGC 8 cut(s) 110, 258, 399, 504, 902, 992, 1083, 1091
SspDI GGCGCC 1 cut(s) 442
SspI AATATT 1 cut(s) 678
SspMI CTAG 4 cut(s) 342, 360, 495, 722
StyD4I CCNGG 3 cut(s) 414, 415, 453
StyI CCWWGG 3 cut(s) 359, 945, 1074
TaaI ACNGT 1 cut(s) 987
TaqI TCGA 2 cut(s) 49, 182
TasI AATT 8 cut(s) 295, 789, 860, 870, 1032, 1055, 1066, 1104
TatI WGTACW 2 cut(s) 232, 338
TauI GCSGC 2 cut(s) 261, 994
TfiI GAWTC 2 cut(s) 162, 836
Tru1I TTAA 5 cut(s) 305, 489, 750, 762, 1031
Tru9I TTAA 5 cut(s) 305, 489, 750, 762, 1031
TscAI CASTG 2 cut(s) 375, 614
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 8 cut(s) 28, 31, 34, 458, 535, 638, 650, 994
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 17, 117, 706, 819
TspGWI ACGGA 4 cut(s) 364, 384, 436, 829
TspMI CCCGGG 1 cut(s) 415
TspRI CASTG 2 cut(s) 375, 614
Vha464I CTTAAG 1 cut(s) 749
XapI RAATTY 1 cut(s) 870
XceI RCATGY 2 cut(s) 19, 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 952
XmaI CCCGGG 1 cut(s) 415
XmaJI CCTAGG 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 76
XspI CTAG 4 cut(s) 342, 360, 495, 722
ZrmI AGTACT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.