MD01G1181900.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010964
Physical Location & Seq
Reverse (-)
28078720 .. 28082280
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1181900.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGAACGACACTTACATGTCACGCAGGCAGCAGCAGCAGCAATCCAGCCCTCGACTTCTCAGAAGCAGAAGTGGAACTACTTCTCCGACCACAACCCTAACCCTAACCATACCGCAAAACATTTCAAGTTCATCATGCACTACCACGCCATGCAACAGCCAAAGATTCACCACCCCCTGCCGCTCGAAATCTATCGTAAAATCAAGAGGCAATAAGGATCACAAGGAAGAGGAAGTACATGCACTCAAGCAAAAGAAGGGCGGCCAAGAAAATATCAGTAGAAAACAAGGTACTAATAATTTAGGTTTAAATACAAGTTTTGCTAAGTTGCAGGGTGGGAGTACTAGTCCAAGTCCACGCCCTCGGATATCAGTGTCCGTGCCACCTCCGAGGTCTCCGTCCGCATGGGCGTTGTCGCCGGGACGGTCTTTGCCATGGAAGGTGGCGCCGGAGTCCCCGGCGGCTGTGCCTTCCACAAGAGCATCTAGGGTTAAGTCTAGTGGTGGCGGGGTTGGTGGGGTTTTGAAGTATTTTAGGCTGCAGAAGAAAGTGTCGCCGGTACAAGAGGAGGACTTCCACCGGTTTCGGCTTTTGCATAATAGGCTACTGCAGTGGAGGTTTGCAAATGCCAGAGCTGAGGCTGCTAAGATTGCTGCACAGAAAAGTTCACAGGATAAAATATTTAGTGTGTGGCTTAGAACATTGAAGGTGAGAAACTTCATCCTAGACAAAAGAATGCAAGTGCAAAAGCTAAAGCATGAGATTAAGGTGTACCAGATTTTGAACCCACAAATTATTCTATTGAATGAATGGGGAAAACTAGATAAAAAAAATCAAGAATCCGTCTGCAGGTTGGTGAGAAAATTGTCTGGAATTTCAAACACAATCCCTTTGGTACATGACGCGGAGGTAGATGTGGAGTCTCTGTACGAATCCATGACCACGGCCATGGCTATGATGGAAGGTATTTTAGGAATGGTCTCTAAACTGTTGCCGCAGCTGGAAAAGGTGCTTTACATGGTTACGGAGCTTTTAATCGCACAAAAACAACAGAGAGAATTGGAGAATAATTTAGCCTTGGTTTCTGCACTTGCGGAGGAGGAGGCAAGTGTAAGAGCACATCTAATCCAAATGGAGTCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

382

Amino Acids

42.68

Weight (kDa)

10.2

Isoelectric Point (pI)

63.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 53 - 353 1.8e-64 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 840
AccB1I GGYRCC 1 cut(s) 445
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 1 cut(s) 905
AciI CCGC 9 cut(s) 113, 181, 261, 402, 461, 507, 905, 995, 1094
AclWI GGATC 1 cut(s) 225
AcoI YGGCCR 2 cut(s) 262, 945
AcsI RAATTY 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 7 cut(s) 237, 292, 343, 561, 773, 897, 929
AfiI CCNNNNNNNGG 1 cut(s) 849
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 579
AgsI TTSAA 6 cut(s) 126, 526, 706, 784, 805, 879
AhlI ACTAGT 1 cut(s) 344
AloI GAACNNNNNNTCC 2 cut(s) 67, 99
AluBI AGCT 4 cut(s) 635, 751, 1000, 1030
AluI AGCT 4 cut(s) 635, 751, 1000, 1030
Alw21I GWGCWC 1 cut(s) 1120
Alw26I GTCTC 3 cut(s) 399, 927, 985
AlwI GGATC 1 cut(s) 225
AoxI GGCC 2 cut(s) 262, 945
ApeKI GCWGC 8 cut(s) 28, 31, 34, 37, 538, 641, 653, 997
ApoI RAATTY 1 cut(s) 873
AsiGI ACCGGT 1 cut(s) 579
Asp700I GAANNNNTTC 1 cut(s) 79
AspLEI GCGC 1 cut(s) 448
AsuC2I CCSGG 2 cut(s) 420, 458
AsuHPI GGTGA 3 cut(s) 160, 721, 868
BanI GGYRCC 1 cut(s) 445
BarI GAAGNNNNNNTAC 4 cut(s) 61, 93, 219, 251
Bbv12I GWGCWC 1 cut(s) 1120
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 8 cut(s) 40, 43, 46, 49, 525, 628, 640, 1009
BccI CCATC 1 cut(s) 952
BceAI ACGGC 1 cut(s) 960
BcnI CCSGG 2 cut(s) 420, 458
BcoDI GTCTC 3 cut(s) 399, 927, 985
BcuI ACTAGT 1 cut(s) 344
BfaI CTAG 5 cut(s) 345, 486, 498, 725, 821
BfmI CTRYAG 3 cut(s) 539, 608, 847
BfoI RGCGCY 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 840
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 2 cut(s) 420, 458
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 2 cut(s) 420, 458
BmsI GCATC 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 420, 458
BsaHI GRCGYC 1 cut(s) 446
BsaI GGTCTC 2 cut(s) 399, 985
BsaJI CCNNGG 7 cut(s) 362, 389, 434, 456, 942, 948, 1077
BsaWI WCCGGW 1 cut(s) 579
BsaXI ACNNNNNCTCC 7 cut(s) 67, 97, 331, 361, 911, 941, 1127
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse118I RCCGGY 2 cut(s) 556, 579
BseDI CCNNGG 7 cut(s) 362, 389, 434, 456, 942, 948, 1077
BseGI GGATG 1 cut(s) 720
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMII CTCAG 2 cut(s) 73, 627
BseRI GAGGAG 3 cut(s) 581, 1112, 1115
BseXI GCAGC 8 cut(s) 40, 43, 46, 49, 525, 628, 640, 1009
BsgI GTGCAG 2 cut(s) 639, 1071
Bsh1236I CGCG 1 cut(s) 905
BshFI GGCC 2 cut(s) 264, 947
BshNI GGYRCC 1 cut(s) 445
BshTI ACCGGT 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 1120
BsiSI CCGG 5 cut(s) 419, 449, 458, 557, 580
BslFI GGGAC 2 cut(s) 435, 439
BslI CCNNNNNNNGG 1 cut(s) 849
BsmAI GTCTC 3 cut(s) 399, 927, 985
BsmFI GGGAC 2 cut(s) 435, 439
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 2 cut(s) 264, 947
Bso31I GGTCTC 2 cut(s) 399, 985
Bsp1286I GDGCHC 1 cut(s) 1120
Bsp143I GATC 1 cut(s) 217
Bsp19I CCATGG 2 cut(s) 434, 948
BspACI CCGC 9 cut(s) 113, 181, 261, 402, 461, 507, 905, 995, 1094
BspANI GGCC 2 cut(s) 264, 947
BspCNI CTCAG 2 cut(s) 72, 628
BspFNI CGCG 1 cut(s) 905
BspLI GGNNCC 1 cut(s) 447
BspMAI CTGCAG 3 cut(s) 543, 612, 851
BspMI ACCTGC 1 cut(s) 840
BspPI GGATC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 445
BspTNI GGTCTC 2 cut(s) 399, 985
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 2 cut(s) 556, 579
BssAI RCCGGY 2 cut(s) 556, 579
BssECI CCNNGG 7 cut(s) 362, 389, 434, 456, 942, 948, 1077
BssMI GATC 1 cut(s) 217
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 3 cut(s) 434, 948, 1077
Bst4CI ACNGT 2 cut(s) 426, 990
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 5 cut(s) 59, 324, 636, 645, 695
BstDSI CCRYGG 3 cut(s) 434, 942, 948
BstF5I GGATG 1 cut(s) 720
BstFNI CGCG 1 cut(s) 905
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 1 cut(s) 220
BstMAI GTCTC 3 cut(s) 399, 927, 985
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 5 cut(s) 34, 37, 601, 641, 650
BstNSI RCATGY 2 cut(s) 19, 242
BstSCI CCNGG 2 cut(s) 418, 456
BstSFI CTRYAG 3 cut(s) 539, 608, 847
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 8 cut(s) 40, 43, 46, 49, 525, 628, 640, 1009
BstXI CCANNNNNNTGG 1 cut(s) 949
BsuRI GGCC 2 cut(s) 264, 947
BtgI CCRYGG 3 cut(s) 434, 942, 948
BtsCI GGATG 1 cut(s) 720
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 2 cut(s) 378, 617
BveI ACCTGC 1 cut(s) 840
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 2 cut(s) 556, 579
CseI GACGC 1 cut(s) 911
Csp6I GTAC 7 cut(s) 236, 291, 342, 560, 772, 896, 928
CspAI ACCGGT 1 cut(s) 579
CviQI GTAC 7 cut(s) 236, 291, 342, 560, 772, 896, 928
DdeI CTNAG 5 cut(s) 59, 324, 636, 645, 695
DinI GGCGCC 1 cut(s) 447
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
DraI TTTAAA 1 cut(s) 309
EaeI YGGCCR 2 cut(s) 262, 945
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco130I CCWWGG 3 cut(s) 434, 948, 1077
Eco31I GGTCTC 2 cut(s) 399, 985
Eco32I GATATC 1 cut(s) 369
EcoRV GATATC 1 cut(s) 369
EcoT14I CCWWGG 3 cut(s) 434, 948, 1077
EgeI GGCGCC 1 cut(s) 447
EheI GGCGCC 1 cut(s) 447
ErhI CCWWGG 3 cut(s) 434, 948, 1077
FaqI GGGAC 2 cut(s) 435, 439
FauI CCCGC 1 cut(s) 500
FokI GGATG 1 cut(s) 707
FspBI CTAG 5 cut(s) 345, 486, 498, 725, 821
GlaI GCGC 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 2 cut(s) 264, 947
HapII CCGG 5 cut(s) 419, 449, 458, 557, 580
HgaI GACGC 1 cut(s) 911
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 446
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 6 cut(s) 165, 452, 839, 920, 932, 1136
HpaII CCGG 5 cut(s) 419, 449, 458, 557, 580
HphI GGTGA 3 cut(s) 160, 721, 868
Hpy166II GTNNAC 3 cut(s) 356, 668, 772
Hpy188I TCNGA 4 cut(s) 62, 87, 366, 390
Hpy188III TCNNGA 3 cut(s) 204, 836, 870
Hpy8I GTNNAC 3 cut(s) 356, 668, 772
HpyAV CCTTC 5 cut(s) 250, 433, 480, 700, 956
HpyCH4III ACNGT 2 cut(s) 426, 990
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 37, 601, 641, 650
HpyF3I CTNAG 5 cut(s) 59, 324, 636, 645, 695
Hsp92I GRCGYC 1 cut(s) 446
HspAI GCGC 1 cut(s) 446
KasI GGCGCC 1 cut(s) 445
Kzo9I GATC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 1027
Lsp1109I GCAGC 8 cut(s) 40, 43, 46, 49, 525, 628, 640, 1009
LweI GCATC 1 cut(s) 491
MaeI CTAG 5 cut(s) 345, 486, 498, 725, 821
MaeIII GTNAC 2 cut(s) 18, 1021
MalI GATC 1 cut(s) 219
MbiI CCGCTC 1 cut(s) 183
MboI GATC 1 cut(s) 217
MboII GAAGA 2 cut(s) 239, 556
MhlI GDGCHC 1 cut(s) 1120
MluCI AATT 6 cut(s) 298, 792, 863, 873, 1058, 1069
Mly113I GGCGCC 1 cut(s) 446
MlyI GAGTC 3 cut(s) 461, 929, 1145
MmeI TCCRAC 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 79
MseI TTAA 4 cut(s) 308, 492, 765, 1034
MslI CAYNNNNRTG 3 cut(s) 14, 947, 953
MspA1I CMGCKG 1 cut(s) 1000
MspI CCGG 5 cut(s) 419, 449, 458, 557, 580
MspR9I CCNGG 2 cut(s) 420, 458
Mva1269I GAATGC 1 cut(s) 741
MvnI CGCG 1 cut(s) 905
MwoI GCNNNNNNNGC 5 cut(s) 34, 37, 601, 641, 650
NarI GGCGCC 1 cut(s) 446
NciI CCSGG 2 cut(s) 420, 458
NcoI CCATGG 2 cut(s) 434, 948
NdeII GATC 1 cut(s) 217
NlaIV GGNNCC 1 cut(s) 447
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 2 cut(s) 19, 242
PciI ACATGT 1 cut(s) 15
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 165, 839, 932
PinAI ACCGGT 1 cut(s) 579
PleI GAGTC 3 cut(s) 460, 928, 1144
PluTI GGCGCC 1 cut(s) 449
PpsI GAGTC 3 cut(s) 460, 928, 1144
PscI ACATGT 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 447
PsrI GAACNNNNNNTAC 1 cut(s) 28
PstI CTGCAG 3 cut(s) 543, 612, 851
PvuII CAGCTG 1 cut(s) 1000
RsaI GTAC 7 cut(s) 237, 292, 343, 561, 773, 897, 929
RsaNI GTAC 7 cut(s) 236, 291, 342, 560, 772, 896, 928
RseI CAYNNNNRTG 3 cut(s) 14, 947, 953
SaqAI TTAA 4 cut(s) 308, 492, 765, 1034
Sau3AI GATC 1 cut(s) 217
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 3 cut(s) 461, 929, 1145
ScrFI CCNGG 2 cut(s) 420, 458
SduI GDGCHC 1 cut(s) 1120
SfaNI GCATC 1 cut(s) 491
SfcI CTRYAG 3 cut(s) 539, 608, 847
SfoI GGCGCC 1 cut(s) 447
SmiMI CAYNNNNRTG 3 cut(s) 14, 947, 953
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SpeI ACTAGT 1 cut(s) 344
Sse9I AATT 6 cut(s) 298, 792, 863, 873, 1058, 1069
SsiI CCGC 9 cut(s) 113, 181, 261, 402, 461, 507, 905, 995, 1094
SspDI GGCGCC 1 cut(s) 445
SspI AATATT 1 cut(s) 681
SspMI CTAG 5 cut(s) 345, 486, 498, 725, 821
StyD4I CCNGG 2 cut(s) 418, 456
StyI CCWWGG 3 cut(s) 434, 948, 1077
TaaI ACNGT 2 cut(s) 426, 990
TaqI TCGA 2 cut(s) 52, 185
TasI AATT 6 cut(s) 298, 792, 863, 873, 1058, 1069
TatI WGTACW 2 cut(s) 235, 341
TauI GCSGC 4 cut(s) 183, 264, 464, 997
TfiI GAWTC 3 cut(s) 165, 839, 932
Tru1I TTAA 4 cut(s) 308, 492, 765, 1034
Tru9I TTAA 4 cut(s) 308, 492, 765, 1034
TscAI CASTG 2 cut(s) 378, 617
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 8 cut(s) 28, 31, 34, 37, 538, 641, 653, 997
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 17, 120, 709, 822
TspGWI ACGGA 4 cut(s) 367, 387, 832, 1040
TspRI CASTG 2 cut(s) 378, 617
XapI RAATTY 1 cut(s) 873
XceI RCATGY 2 cut(s) 19, 242
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmnI GAANNNNTTC 1 cut(s) 79
XspI CTAG 5 cut(s) 345, 486, 498, 725, 821
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.