FvH4_7g27280

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010964
Physical Location & Seq
Reverse (-)
20423796 .. 20426367
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27280.t1

Sequence Viewer

Length: 1161 bp
ATGGAAAACACATACCCGCGGAGGCACCAATCACACACCATGCACACCACGTCACAGCAGCACTCTTCTGGTCCTGATCGTCTTCTCCGCAGCAGAAGCGGAAGCAGTACTTCTCCGGCGATGAACAACTTGTTATACACTCCGAGCGCCAGCCAGAGATTCACCACTCCACGGTCGAAGTCCACCGCCAAATCCAGAACCAATTACAGAGATGAGGAGAAAACGGTAATTTCGAGCATGAAAAAGATGGGACAAGAGAATAGAGAAGGTAATACTCAGCATGTTACTTTTGCTAGCCCTAAGTTGATGCAACGCGGGAACACTAGCCCTAGTCCGAGTCCACGCCCTAGCTCAACAAAGGGGAGGAAGTCGTCGACGTCTCCTTCGGCTTGGGCGTTATCTCCCGGAAGGTCTTTGCCGTTCGCGGTGGGGCCGGAGGCGACTGCGGCGGCAGTAGTGGCTTCTCCGAGACCGAGGGTGAAGTCCAGTGGGGGTGGAGTTGGTGGGGTGTTGAAGTATTTTAGGCTGCAGAAGAAAGGGTCGAGTATACAAGAAGAGGAGTATCATCAGTTTCGGGTTTTGCATAATAGGTTGCTCCAATGGAGGTTTGCCAATGCTAGGGCAAGGGCTGCCATGGTCGCATCACACAAAGTTGCACGGGATAGAATGTTCAGCGTGTGGCTTAGAATTTCCAAAATGAGAAACTATGTTTTAGATAAGCAACTACAAGTGGAGAAGCTCAAGCATCAGATGAAGATTTATCAAATTGTGAACTCTCAAATTTCTCTACTGAATGAATGGGGAAAATTGGAGAAGAAAAATATAGAATCTGTGAGCAGGATCGTGAGGAAGTTATCTGGAATTTCAAACACAATTCCTTTGGTAAATGACGCTAAGGGAGATGCGGCGTCTATACATGAAGCAATGATCACAGCCATGGATGTCATGGCTGGTATTCAACCAACGGTCACCAAATTACTCCCACAGCTCGAAAAGCTACTTTACATGGTTACGGAGCTTCTAATCATGCAGAAGCAGCAAAGGGAACTGGAGAAGTATATGACATCGGTCGTTGGTCTTATGAAAGAGGAGACGAGCGTAAGAGTACAACTCATCCAAGTAGCGAGTGAAGGAGAGGCCCGGATTCATATGTTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

387

Amino Acids

43.36

Weight (kDa)

10.5

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 38 - 350 5.2e-69 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 24
AccI GTMKAC 2 cut(s) 374, 547
AccII CGCG 3 cut(s) 19, 315, 425
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 3 cut(s) 687, 780, 861
AcyI GRCGYC 2 cut(s) 377, 908
AfaI GTAC 2 cut(s) 109, 1107
AfiI CCNNNNNNNGG 2 cut(s) 171, 618
AgsI TTSAA 4 cut(s) 514, 867, 959, 1156
AjiI CACGTC 1 cut(s) 51
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 5 cut(s) 351, 739, 988, 997, 1018
AluI AGCT 5 cut(s) 351, 739, 988, 997, 1018
Alw26I GTCTC 3 cut(s) 384, 463, 1085
AlwI GGATC 1 cut(s) 848
AoxI GGCC 2 cut(s) 431, 1137
ApeKI GCWGC 5 cut(s) 58, 90, 526, 629, 1036
ApoI RAATTY 3 cut(s) 687, 780, 861
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 3 cut(s) 71, 431, 1138
AsuC2I CCSGG 2 cut(s) 405, 1141
AsuHPI GGTGA 3 cut(s) 154, 490, 961
AsuNHI GCTAGC 1 cut(s) 293
AvaII GGWCC 1 cut(s) 71
BanI GGYRCC 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 74
BbvI GCAGC 5 cut(s) 70, 102, 513, 616, 1048
BccI CCATC 1 cut(s) 241
BceAI ACGGC 1 cut(s) 403
BclI TGATCA 1 cut(s) 927
BcnI CCSGG 2 cut(s) 405, 1141
BcoDI GTCTC 3 cut(s) 384, 463, 1085
BfaI CTAG 5 cut(s) 294, 324, 330, 348, 618
BfmI CTRYAG 1 cut(s) 527
BfoI RGCGCY 1 cut(s) 150
BisI GCNGC 8 cut(s) 59, 91, 447, 450, 527, 630, 906, 1037
BlsI GCNGC 8 cut(s) 60, 92, 448, 451, 528, 631, 907, 1038
BmcAI AGTACT 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 405, 1141
Bme18I GGWCC 1 cut(s) 71
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 71, 431, 1138
BmiI GGNNCC 2 cut(s) 26, 432
BmrFI CCNGG 2 cut(s) 405, 1141
BmsI GCATC 4 cut(s) 297, 650, 754, 892
BmtI GCTAGC 1 cut(s) 297
BoxI GACNNNNGTC 1 cut(s) 1067
BpiI GAAGAC 1 cut(s) 74
BplI GAGNNNNNCTC 2 cut(s) 1095, 1127
BpmI CTGGAG 1 cut(s) 1070
Bpu10I CCTNAGC 1 cut(s) 894
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 2 cut(s) 405, 1141
BsaHI GRCGYC 2 cut(s) 377, 908
BsaI GGTCTC 1 cut(s) 463
BsaJI CCNNGG 5 cut(s) 17, 170, 473, 633, 936
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 618
Bse1I ACTGG 2 cut(s) 486, 1053
Bse3DI GCAATG 1 cut(s) 930
BseDI CCNNGG 5 cut(s) 17, 170, 473, 633, 936
BseGI GGATG 2 cut(s) 946, 1113
BseLI CCNNNNNNNGG 2 cut(s) 171, 618
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 2 cut(s) 486, 1053
BseRI GAGGAG 3 cut(s) 230, 572, 1103
BseXI GCAGC 5 cut(s) 70, 102, 513, 616, 1048
Bsh1236I CGCG 3 cut(s) 19, 315, 425
Bsh1285I CGRYCG 2 cut(s) 176, 1071
BshFI GGCC 2 cut(s) 433, 1139
BshNI GGYRCC 1 cut(s) 24
BsiEI CGRYCG 2 cut(s) 176, 1071
BsiSI CCGG 4 cut(s) 116, 405, 434, 1141
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 171, 618
BsmAI GTCTC 3 cut(s) 384, 463, 1085
BsmBI CGTCTC 2 cut(s) 384, 1085
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 2 cut(s) 433, 1139
Bso31I GGTCTC 1 cut(s) 463
Bsp143I GATC 3 cut(s) 76, 840, 927
Bsp19I CCATGG 2 cut(s) 633, 936
BspANI GGCC 2 cut(s) 433, 1139
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 3 cut(s) 19, 315, 425
BspLI GGNNCC 2 cut(s) 26, 432
BspMAI CTGCAG 1 cut(s) 531
BspOI GCTAGC 1 cut(s) 297
BspPI GGATC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 24
BspTNI GGTCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 930
BsrI ACTGG 2 cut(s) 486, 1053
BssECI CCNNGG 5 cut(s) 17, 170, 473, 633, 936
BssMI GATC 3 cut(s) 76, 840, 927
BssNAI GTATAC 1 cut(s) 548
BssNI GRCGYC 2 cut(s) 377, 908
BssT1I CCWWGG 2 cut(s) 633, 936
Bst1107I GTATAC 1 cut(s) 548
Bst4CI ACNGT 3 cut(s) 174, 226, 967
Bst6I CTCTTC 2 cut(s) 70, 549
BstACI GRCGYC 2 cut(s) 377, 908
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 151, 295
BstDEI CTNAG 4 cut(s) 276, 300, 683, 894
BstDSI CCRYGG 4 cut(s) 17, 170, 633, 936
BstEII GGTNACC 1 cut(s) 967
BstF5I GGATG 2 cut(s) 946, 1113
BstFNI CGCG 3 cut(s) 19, 315, 425
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 79, 843, 930
BstMAI GTCTC 3 cut(s) 384, 463, 1085
BstMBI GATC 3 cut(s) 76, 840, 927
BstMCI CGRYCG 2 cut(s) 176, 1071
BstMWI GCNNNNNNNGC 7 cut(s) 96, 446, 458, 629, 638, 994, 1036
BstNSI RCATGY 1 cut(s) 284
BstPAI GACNNNNGTC 1 cut(s) 1067
BstPI GGTNACC 1 cut(s) 967
BstSCI CCNGG 2 cut(s) 403, 1139
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 3 cut(s) 19, 315, 425
BstV1I GCAGC 5 cut(s) 70, 102, 513, 616, 1048
BstV2I GAAGAC 1 cut(s) 74
BstZ17I GTATAC 1 cut(s) 548
BsuRI GGCC 2 cut(s) 433, 1139
BtgI CCRYGG 4 cut(s) 17, 170, 633, 936
BtgZI GCGATG 1 cut(s) 134
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 2 cut(s) 946, 1113
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 2 cut(s) 151, 295
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 3 cut(s) 71, 431, 1138
Cfr42I CCGCGG 1 cut(s) 20
CseI GACGC 2 cut(s) 897, 899
Csp6I GTAC 2 cut(s) 108, 1106
CviAII CATG 9 cut(s) 40, 238, 281, 634, 917, 937, 946, 1006, 1027
CviQI GTAC 2 cut(s) 108, 1106
DdeI CTNAG 4 cut(s) 276, 300, 683, 894
DpnI GATC 3 cut(s) 78, 842, 929
DpnII GATC 3 cut(s) 76, 840, 927
Eam1104I CTCTTC 2 cut(s) 70, 549
EarI CTCTTC 2 cut(s) 70, 549
Eco130I CCWWGG 2 cut(s) 633, 936
Eco31I GGTCTC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 71
Eco91I GGTNACC 1 cut(s) 967
EcoO65I GGTNACC 1 cut(s) 967
EcoT14I CCWWGG 2 cut(s) 633, 936
ErhI CCWWGG 2 cut(s) 633, 936
Esp3I CGTCTC 2 cut(s) 384, 1085
FaeI CATG 9 cut(s) 43, 241, 284, 637, 920, 940, 949, 1009, 1030
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FaqI GGGAC 1 cut(s) 264
FatI CATG 9 cut(s) 39, 237, 280, 633, 916, 936, 945, 1005, 1026
FauI CCCGC 2 cut(s) 24, 308
FauNDI CATATG 1 cut(s) 1149
FbaI TGATCA 1 cut(s) 927
FblI GTMKAC 2 cut(s) 374, 547
Fnu4HI GCNGC 8 cut(s) 59, 91, 447, 450, 527, 630, 906, 1037
FokI GGATG 2 cut(s) 953, 1100
Fsp4HI GCNGC 8 cut(s) 59, 91, 447, 450, 527, 630, 906, 1037
FspBI CTAG 5 cut(s) 294, 324, 330, 348, 618
GlaI GCGC 1 cut(s) 148
GluI GCNGC 8 cut(s) 59, 91, 447, 450, 527, 630, 906, 1037
GsuI CTGGAG 1 cut(s) 1070
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 433, 1139
HapII CCGG 4 cut(s) 116, 405, 434, 1141
HgaI GACGC 2 cut(s) 897, 899
HhaI GCGC 1 cut(s) 149
Hin1I GRCGYC 2 cut(s) 377, 908
Hin1II CATG 9 cut(s) 43, 241, 284, 637, 920, 940, 949, 1009, 1030
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 4 cut(s) 159, 337, 827, 1144
HpaII CCGG 4 cut(s) 116, 405, 434, 1141
HphI GGTGA 3 cut(s) 154, 490, 961
Hpy166II GTNNAC 5 cut(s) 183, 341, 375, 548, 772
Hpy188I TCNGA 4 cut(s) 144, 336, 468, 750
Hpy188III TCNNGA 4 cut(s) 74, 195, 844, 858
Hpy8I GTNNAC 5 cut(s) 183, 341, 375, 548, 772
Hpy99I CGWCG 2 cut(s) 376, 379
HpyAV CCTTC 4 cut(s) 260, 393, 402, 1124
HpyCH4III ACNGT 3 cut(s) 174, 226, 967
HpyCH4IV ACGT 2 cut(s) 50, 377
HpyCH4V TGCA 6 cut(s) 43, 310, 529, 583, 656, 1030
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 446, 458, 629, 638, 994, 1036
HpyF3I CTNAG 4 cut(s) 276, 300, 683, 894
HpySE526I ACGT 2 cut(s) 50, 377
Hsp92I GRCGYC 2 cut(s) 377, 908
Hsp92II CATG 9 cut(s) 43, 241, 284, 637, 920, 940, 949, 1009, 1030
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 927
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 3 cut(s) 76, 840, 927
LmnI GCTCC 2 cut(s) 600, 1015
Lsp1109I GCAGC 5 cut(s) 70, 102, 513, 616, 1048
LweI GCATC 4 cut(s) 297, 650, 754, 892
MaeI CTAG 5 cut(s) 294, 324, 330, 348, 618
MaeII ACGT 2 cut(s) 50, 377
MaeIII GTNAC 4 cut(s) 51, 283, 967, 1009
MalI GATC 3 cut(s) 78, 842, 929
MboI GATC 3 cut(s) 76, 840, 927
MboII GAAGA 6 cut(s) 57, 74, 544, 566, 766, 826
MlyI GAGTC 1 cut(s) 346
MslI CAYNNNNRTG 1 cut(s) 935
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 4 cut(s) 116, 405, 434, 1141
MspR9I CCNGG 2 cut(s) 405, 1141
MvnI CGCG 3 cut(s) 19, 315, 425
MwoI GCNNNNNNNGC 7 cut(s) 96, 446, 458, 629, 638, 994, 1036
NciI CCSGG 2 cut(s) 405, 1141
NcoI CCATGG 2 cut(s) 633, 936
NdeI CATATG 1 cut(s) 1149
NdeII GATC 3 cut(s) 76, 840, 927
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 9 cut(s) 43, 241, 284, 637, 920, 940, 949, 1009, 1030
NlaIV GGNNCC 2 cut(s) 26, 432
NmuCI GTSAC 2 cut(s) 51, 967
NspI RCATGY 1 cut(s) 284
PcsI WCGNNNNNNNCGW 2 cut(s) 230, 392
PfeI GAWTC 3 cut(s) 159, 827, 1144
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 8 cut(s) 60, 92, 448, 451, 528, 631, 907, 1038
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 1067
PspEI GGTNACC 1 cut(s) 967
PspN4I GGNNCC 2 cut(s) 26, 432
PspPI GGNCC 3 cut(s) 71, 431, 1138
PstI CTGCAG 1 cut(s) 531
RsaI GTAC 2 cut(s) 109, 1107
RsaNI GTAC 2 cut(s) 108, 1106
RseI CAYNNNNRTG 1 cut(s) 935
SacII CCGCGG 1 cut(s) 20
SalI GTCGAC 1 cut(s) 373
SatI GCNGC 8 cut(s) 59, 91, 447, 450, 527, 630, 906, 1037
Sau3AI GATC 3 cut(s) 76, 840, 927
Sau96I GGNCC 3 cut(s) 71, 431, 1138
ScaI AGTACT 1 cut(s) 109
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 405, 1141
SfaNI GCATC 4 cut(s) 297, 650, 754, 892
SfcI CTRYAG 1 cut(s) 527
Sfr303I CCGCGG 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 20
SgrDI CGTCGACG 1 cut(s) 373
SinI GGWCC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
SspMI CTAG 5 cut(s) 294, 324, 330, 348, 618
StyD4I CCNGG 2 cut(s) 403, 1139
StyI CCWWGG 2 cut(s) 633, 936
TaaI ACNGT 3 cut(s) 174, 226, 967
TaiI ACGT 2 cut(s) 53, 380
TaqI TCGA 5 cut(s) 176, 233, 374, 542, 990
TaqII GACCGA 2 cut(s) 487, 1057
TatI WGTACW 2 cut(s) 107, 1105
TauI GCSGC 3 cut(s) 449, 452, 908
TfiI GAWTC 3 cut(s) 159, 827, 1144
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 2 cut(s) 51, 967
TseI GCWGC 5 cut(s) 58, 90, 526, 629, 1036
Tsp45I GTSAC 2 cut(s) 51, 967
TspDTI ATGAA 7 cut(s) 137, 254, 767, 810, 933, 1097, 1136
TspGWI ACGGA 1 cut(s) 1028
TspRI CASTG 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 3 cut(s) 687, 780, 861
XceI RCATGY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XmiI GTMKAC 2 cut(s) 374, 547
XspI CTAG 5 cut(s) 294, 324, 330, 348, 618
ZraI GACGTC 1 cut(s) 378
ZrmI AGTACT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.