Rw1G034400

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010964
Physical Location & Seq
Forward (+)
62307511 .. 62318403
10893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034400.1

Sequence Viewer

Length: 1233 bp
ATGGAAAACACATACCCGCGGAGGCTCCAATCACATACAGCCACGTCACAACAGCACTCTTCTGGGCCTCGTCTTCTACGCAGCAGAAGCGGAAGTAGTACTTCTCCGGCGATAAACAACTCGTTATACTCTCCGAGCCCCAGCCACAGATTCACCACTCCCCGGTCGAAGTCCACCGCCAAATCCAGAACCGGTTACAGAGATGAAGAGAAAAATGTAACTTCCAGCATGCAAAAGAAGGGGCAAGAAAATAGAGAAGGTAAAAATAATAGTCAGAATACTACTTTTGCTATCCCTAGGTTGTTGCTGCAGCGCGGGAATACTAGCCCTAGTCCTAATCCGCGCCCTTGCTTTGGCTCGACGAAGGGGAGGAAGTCGCCGGTGTCTCCTTCGGCTTGGGCGTTGTCTCCGGGACGGTCTTTGCCATGCACGGTGGGACCGGAGCTGACTGCGGCGGCAGTGGCTTCGCCGAGACCGAGGGTGAAGTCTAGTGGGGGTGGAGTTGGTGGGGTTTTGAAGTATTTTAGGCTTCCGAAGAAAGGGATGACTATACAAGAGGAGGAGTTTCATCAGTTTCGGATTTTGCATAATAGGTTGCTCCAATGGAGGTTTGCCAATGCTCGAGCTCAAGCTGCCATAGTTGCTTCACACAAAGTTGCAAAGGACAGAATGTTCAGTGTGTGGCTTAGAATTTTCAAAATGAGGAACTACATCCTAGATAAGCAACTACAACTGCAAAAGCTCAAGCATCAAGTGAAGGTGTATCAAATTGTGTACCCTCAAATTTCTCTACTGAACGAATGGGGGAAATTGGAGAGAAAAAATCAAGAATCTGTGAGCAGGATGGTGAGGAAGTTATCCGGAATTTCAAATACAATTCCTTTGGTAAATGATGCTAAGGGAGATGTGGCGTCTATATACGAAGCGATGATCACGGCCATGGATGTCATGGCCGGTATTCAAGCAACGGTCACTAAATTGCTCCCGCAGCTCGAAAAGCTGCTCTACATGGTTACGGAGCTTTTGATCATGCAGAAGCAACAAAGAGAATTAGAGAAATATATTACATCGGTCGTTGGTCTTATGCTCTTGATAGCAATCTACCTAGCCTTTGGTGTGAAATGTGAACTCAACCATTTACTCATAAGTCTAAAAGACTTGACCCTTCCCCTTTCTATGCTTAATTCTTCAAATCTTCGAAGATTGGAGGTACTATTGCTCACCAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

410

Amino Acids

46.01

Weight (kDa)

10.44

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 32 - 351 2.1e-68 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 19, 315, 343
AccIII TCCGGA 1 cut(s) 860
AciI CCGC 9 cut(s) 17, 19, 90, 177, 315, 341, 452, 455, 986
AcoI YGGCCR 2 cut(s) 936, 951
AcsI RAATTY 3 cut(s) 690, 783, 864
AcyI GRCGYC 1 cut(s) 911
AfaI GTAC 3 cut(s) 100, 776, 1212
AfiI CCNNNNNNNGG 3 cut(s) 162, 353, 539
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 5 cut(s) 517, 697, 870, 962, 1191
AjiI CACGTC 1 cut(s) 45
AloI GAACNNNNNNTCC 2 cut(s) 656, 688
AluBI AGCT 7 cut(s) 445, 626, 632, 742, 991, 1000, 1021
AluI AGCT 7 cut(s) 445, 626, 632, 742, 991, 1000, 1021
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 3 cut(s) 390, 411, 466
Ama87I CYCGRG 1 cut(s) 621
Aor13HI TCCGGA 1 cut(s) 860
AoxI GGCC 3 cut(s) 65, 936, 951
ApeKI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
ApoI RAATTY 3 cut(s) 690, 783, 864
AsiGI ACCGGT 1 cut(s) 191
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 2 cut(s) 315, 345
AspS9I GGNCC 2 cut(s) 65, 437
AsuC2I CCSGG 2 cut(s) 163, 411
AsuHPI GGTGA 4 cut(s) 145, 493, 859, 1213
AsuII TTCGAA 1 cut(s) 1198
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 296
BanII GRGCYC 2 cut(s) 140, 628
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BccI CCATC 1 cut(s) 838
BceAI ACGGC 1 cut(s) 951
BcgI CGANNNNNNTGC 2 cut(s) 447, 481
BclI TGATCA 2 cut(s) 930, 1026
BcnI CCSGG 2 cut(s) 163, 411
BcoDI GTCTC 3 cut(s) 390, 411, 466
BfaI CTAG 6 cut(s) 297, 324, 330, 489, 716, 1106
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
BlnI CCTAGG 1 cut(s) 296
BlsI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
BmcAI AGTACT 1 cut(s) 100
Bme1390I CCNGG 2 cut(s) 163, 411
Bme18I GGWCC 1 cut(s) 437
BmeT110I CYCGRG 1 cut(s) 621
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 65, 437
BmiI GGNNCC 2 cut(s) 26, 438
BmrFI CCNGG 2 cut(s) 163, 411
BmsI GCATC 2 cut(s) 757, 883
BpiI GAAGAC 1 cut(s) 65
Bpu10I CCTNAGC 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 1198
BpuEI CTTGAG 2 cut(s) 612, 728
BpuMI CCSGG 2 cut(s) 163, 411
BsaBI GATNNNNATC 1 cut(s) 1097
BsaHI GRCGYC 1 cut(s) 911
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 5 cut(s) 17, 161, 296, 476, 939
BsaWI WCCGGW 3 cut(s) 191, 439, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 162, 353, 539
Bse118I RCCGGY 3 cut(s) 191, 379, 953
Bse8I GATNNNNATC 1 cut(s) 1097
BseAI TCCGGA 1 cut(s) 860
BseDI CCNNGG 5 cut(s) 17, 161, 296, 476, 939
BseGI GGATG 4 cut(s) 549, 711, 849, 949
BseJI GATNNNNATC 1 cut(s) 1097
BseLI CCNNNNNNNGG 3 cut(s) 162, 353, 539
BseRI GAGGAG 2 cut(s) 572, 575
BseXI GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 3 cut(s) 19, 315, 343
Bsh1285I CGRYCG 2 cut(s) 167, 1074
BshFI GGCC 3 cut(s) 67, 938, 953
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 2 cut(s) 167, 1074
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 8 cut(s) 107, 163, 192, 380, 410, 440, 861, 954
BslFI GGGAC 2 cut(s) 426, 450
BslI CCNNNNNNNGG 3 cut(s) 162, 353, 539
BsmAI GTCTC 3 cut(s) 390, 411, 466
BsmFI GGGAC 2 cut(s) 426, 450
BsnI GGCC 3 cut(s) 67, 938, 953
Bso31I GGTCTC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 621
Bsp119I TTCGAA 1 cut(s) 1198
Bsp1286I GDGCHC 2 cut(s) 140, 628
Bsp13I TCCGGA 1 cut(s) 860
Bsp143I GATC 2 cut(s) 930, 1026
Bsp19I CCATGG 1 cut(s) 939
BspACI CCGC 9 cut(s) 17, 19, 90, 177, 315, 341, 452, 455, 986
BspANI GGCC 3 cut(s) 67, 938, 953
BspEI TCCGGA 1 cut(s) 860
BspFNI CGCG 3 cut(s) 19, 315, 343
BspHI TCATGA 1 cut(s) 1229
BspLI GGNNCC 2 cut(s) 26, 438
BspMAI CTGCAG 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 1198
BspTNI GGTCTC 1 cut(s) 466
BsrFI RCCGGY 3 cut(s) 191, 379, 953
BssAI RCCGGY 3 cut(s) 191, 379, 953
BssECI CCNNGG 5 cut(s) 17, 161, 296, 476, 939
BssMI GATC 2 cut(s) 930, 1026
BssNI GRCGYC 1 cut(s) 911
BssT1I CCWWGG 2 cut(s) 296, 939
Bst4CI ACNGT 3 cut(s) 417, 433, 970
Bst6I CTCTTC 2 cut(s) 64, 201
BstACI GRCGYC 1 cut(s) 911
BstBI TTCGAA 1 cut(s) 1198
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 2 cut(s) 686, 897
BstDSI CCRYGG 2 cut(s) 17, 939
BstF5I GGATG 4 cut(s) 549, 711, 849, 949
BstFNI CGCG 3 cut(s) 19, 315, 343
BstHHI GCGC 2 cut(s) 315, 345
BstKTI GATC 2 cut(s) 933, 1029
BstMAI GTCTC 3 cut(s) 390, 411, 466
BstMBI GATC 2 cut(s) 930, 1026
BstMCI CGRYCG 2 cut(s) 167, 1074
BstMWI GCNNNNNNNGC 6 cut(s) 87, 461, 632, 641, 988, 997
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 161, 409
BstSFI CTRYAG 1 cut(s) 308
BstUI CGCG 3 cut(s) 19, 315, 343
BstV1I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
BstV2I GAAGAC 1 cut(s) 65
BsuRI GGCC 3 cut(s) 67, 938, 953
BtgI CCRYGG 2 cut(s) 17, 939
BtgZI GCGATG 1 cut(s) 941
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 549, 711, 849, 949
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 465, 682
Cac8I GCNNGC 1 cut(s) 230
CciI TCATGA 1 cut(s) 1229
CfoI GCGC 2 cut(s) 315, 345
Cfr10I RCCGGY 3 cut(s) 191, 379, 953
Cfr13I GGNCC 2 cut(s) 65, 437
Cfr42I CCGCGG 1 cut(s) 20
CseI GACGC 1 cut(s) 900
Csp6I GTAC 3 cut(s) 99, 775, 1211
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 7 cut(s) 229, 426, 940, 949, 1009, 1030, 1230
CviQI GTAC 3 cut(s) 99, 775, 1211
DdeI CTNAG 2 cut(s) 686, 897
DpnI GATC 2 cut(s) 932, 1028
DpnII GATC 2 cut(s) 930, 1026
EaeI YGGCCR 2 cut(s) 936, 951
Eam1104I CTCTTC 2 cut(s) 64, 201
EarI CTCTTC 2 cut(s) 64, 201
Ecl136II GAGCTC 1 cut(s) 626
Eco130I CCWWGG 2 cut(s) 296, 939
Eco24I GRGCYC 2 cut(s) 140, 628
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 621
EcoICRI GAGCTC 1 cut(s) 626
EcoT14I CCWWGG 2 cut(s) 296, 939
EcoT38I GRGCYC 2 cut(s) 140, 628
ErhI CCWWGG 2 cut(s) 296, 939
FaeI CATG 7 cut(s) 232, 429, 943, 952, 1012, 1033, 1233
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FaqI GGGAC 2 cut(s) 426, 450
FatI CATG 7 cut(s) 228, 425, 939, 948, 1008, 1029, 1229
FauI CCCGC 3 cut(s) 24, 308, 993
FbaI TGATCA 2 cut(s) 930, 1026
Fnu4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FokI GGATG 4 cut(s) 556, 698, 856, 956
FriOI GRGCYC 2 cut(s) 140, 628
Fsp4HI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
FspBI CTAG 6 cut(s) 297, 324, 330, 489, 716, 1106
GlaI GCGC 2 cut(s) 314, 344
GluI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 3 cut(s) 67, 938, 953
HapII CCGG 8 cut(s) 107, 163, 192, 380, 410, 440, 861, 954
HgaI GACGC 1 cut(s) 900
HhaI GCGC 2 cut(s) 315, 345
Hin1I GRCGYC 1 cut(s) 911
Hin1II CATG 7 cut(s) 232, 429, 943, 952, 1012, 1033, 1233
Hin6I GCGC 2 cut(s) 313, 343
HinP1I GCGC 2 cut(s) 313, 343
HinfI GANTC 2 cut(s) 150, 830
HpaII CCGG 8 cut(s) 107, 163, 192, 380, 410, 440, 861, 954
HphI GGTGA 4 cut(s) 145, 493, 859, 1213
Hpy166II GTNNAC 3 cut(s) 174, 775, 1127
Hpy188I TCNGA 4 cut(s) 135, 276, 534, 579
Hpy188III TCNNGA 5 cut(s) 186, 827, 861, 1090, 1230
Hpy8I GTNNAC 3 cut(s) 174, 775, 1127
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 6 cut(s) 232, 251, 358, 399, 751, 1175
HpyCH4III ACNGT 3 cut(s) 417, 433, 970
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 7 cut(s) 232, 310, 429, 586, 659, 736, 1033
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 461, 632, 641, 988, 997
HpyF3I CTNAG 2 cut(s) 686, 897
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 911
Hsp92II CATG 7 cut(s) 232, 429, 943, 952, 1012, 1033, 1233
HspAI GCGC 2 cut(s) 313, 343
Kpn2I TCCGGA 1 cut(s) 860
Ksp22I TGATCA 2 cut(s) 930, 1026
KspI CCGCGG 1 cut(s) 20
Kzo9I GATC 2 cut(s) 930, 1026
LmnI GCTCC 5 cut(s) 30, 442, 603, 987, 1018
Lsp1109I GCAGC 6 cut(s) 93, 294, 322, 619, 987, 1000
LweI GCATC 2 cut(s) 757, 883
MaeI CTAG 6 cut(s) 297, 324, 330, 489, 716, 1106
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 5 cut(s) 45, 194, 217, 970, 1012
MalI GATC 2 cut(s) 932, 1028
MboI GATC 2 cut(s) 930, 1026
MboII GAAGA 7 cut(s) 51, 65, 218, 547, 1179, 1187, 1212
MhlI GDGCHC 2 cut(s) 140, 628
MroI TCCGGA 1 cut(s) 860
MseI TTAA 1 cut(s) 1182
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 8 cut(s) 107, 163, 192, 380, 410, 440, 861, 954
MspR9I CCNGG 2 cut(s) 163, 411
MvnI CGCG 3 cut(s) 19, 315, 343
MwoI GCNNNNNNNGC 6 cut(s) 87, 461, 632, 641, 988, 997
NciI CCSGG 2 cut(s) 163, 411
NcoI CCATGG 1 cut(s) 939
NdeII GATC 2 cut(s) 930, 1026
NlaIII CATG 7 cut(s) 232, 429, 943, 952, 1012, 1033, 1233
NlaIV GGNNCC 2 cut(s) 26, 438
NmeAIII GCCGAG 1 cut(s) 495
NmuCI GTSAC 2 cut(s) 45, 970
NspI RCATGY 1 cut(s) 232
NspV TTCGAA 1 cut(s) 1198
PaeI GCATGC 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 621
PagI TCATGA 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 2 cut(s) 398, 473
PfeI GAWTC 2 cut(s) 150, 830
PfoI TCCNGGA 1 cut(s) 409
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 8 cut(s) 83, 309, 312, 454, 457, 634, 990, 1002
Psp124BI GAGCTC 1 cut(s) 628
PspFI CCCAGC 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 26, 438
PspPI GGNCC 2 cut(s) 65, 437
PspXI VCTCGAGB 1 cut(s) 621
PstI CTGCAG 1 cut(s) 312
RsaI GTAC 3 cut(s) 100, 776, 1212
RsaNI GTAC 3 cut(s) 99, 775, 1211
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 1 cut(s) 628
SacII CCGCGG 1 cut(s) 20
SaqAI TTAA 1 cut(s) 1182
SatI GCNGC 8 cut(s) 82, 308, 311, 453, 456, 633, 989, 1001
Sau3AI GATC 2 cut(s) 930, 1026
Sau96I GGNCC 2 cut(s) 65, 437
ScaI AGTACT 1 cut(s) 100
ScrFI CCNGG 2 cut(s) 163, 411
SduI GDGCHC 2 cut(s) 140, 628
SfaNI GCATC 2 cut(s) 757, 883
SfcI CTRYAG 1 cut(s) 308
Sfr274I CTCGAG 1 cut(s) 621
Sfr303I CCGCGG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 1198
SgrAI CRCCGGYG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 20
SinI GGWCC 1 cut(s) 437
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 621, 627, 743
SmoI CTYRAG 3 cut(s) 621, 627, 743
SphI GCATGC 1 cut(s) 232
SsiI CCGC 9 cut(s) 17, 19, 90, 177, 315, 341, 452, 455, 986
SspMI CTAG 6 cut(s) 297, 324, 330, 489, 716, 1106
SstI GAGCTC 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 161, 409
StyI CCWWGG 2 cut(s) 296, 939
TaaI ACNGT 3 cut(s) 417, 433, 970
TaiI ACGT 1 cut(s) 47
TaqI TCGA 5 cut(s) 167, 359, 622, 993, 1198
TaqII GACCGA 2 cut(s) 490, 1060
TatI WGTACW 1 cut(s) 98
TauI GCSGC 2 cut(s) 455, 458
TfiI GAWTC 2 cut(s) 150, 830
Tru1I TTAA 1 cut(s) 1182
Tru9I TTAA 1 cut(s) 1182
TscAI CASTG 2 cut(s) 465, 682
TseFI GTSAC 2 cut(s) 45, 970
TseI GCWGC 6 cut(s) 81, 307, 310, 632, 988, 1000
Tsp45I GTSAC 2 cut(s) 45, 970
TspDTI ATGAA 3 cut(s) 219, 557, 1218
TspGWI ACGGA 1 cut(s) 1031
TspRI CASTG 2 cut(s) 465, 682
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 3 cut(s) 690, 783, 864
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XhoI CTCGAG 1 cut(s) 621
XmaJI CCTAGG 1 cut(s) 296
XspI CTAG 6 cut(s) 297, 324, 330, 489, 716, 1106
ZrmI AGTACT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.