pycom07g22450

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010964
Physical Location & Seq
Reverse (-)
23794144 .. 23794766
623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22450.1

Sequence Viewer

Length: 321 bp
ATGGAGTCTGCTATTGTGCTTATTTGTGTTCAAGATAAATTGTTTAGTGTGTGGCTTAGAACATTGAAAGTGAGAAACTTCATCCTAGACAAAAGAATACAAGTGCAAAAGCTCAAGCATGAGATTAAGAATCAAGAATCCATCAGCAGGTTGGTGAAAAAATTGTCAGGAATTTCAAACACAATCCCTTTGGTCAATGATGCTAACGCAGATGTAGCATCTGTATACGAAACCATGACCGCGGCCATGGCTGTGATGGAAGGTAATTTAGGAATGGTCTCTAAACTGCTGCCGCAGGTTAGTTCCTCTTTATACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

107

Amino Acids

11.81

Weight (kDa)

9.42

Isoelectric Point (pI)

19.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 10 - 43 1.6e-06 QWRF family
QWRF PF04484 41 - 102 3.2e-08 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 138, 286
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 242
AciI CCGC 3 cut(s) 240, 242, 293
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 3 cut(s) 32, 67, 177
AluBI AGCT 1 cut(s) 112
AluI AGCT 1 cut(s) 112
Alw26I GTCTC 1 cut(s) 283
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 1 cut(s) 171
AsuHPI GGTGA 1 cut(s) 166
BbvI GCAGC 1 cut(s) 276
BccI CCATC 2 cut(s) 149, 250
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 1 cut(s) 86
BfuAI ACCTGC 2 cut(s) 138, 286
BisI GCNGC 3 cut(s) 243, 290, 293
BlsI GCNGC 3 cut(s) 244, 291, 294
BmsI GCATC 2 cut(s) 190, 227
BpuEI CTTGAG 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 283
BsaJI CCNNGG 2 cut(s) 240, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 240, 246
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 147
BseXI GCAGC 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 242
BshFI GGCC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 283
BsnI GGCC 1 cut(s) 245
Bso31I GGTCTC 1 cut(s) 283
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 3 cut(s) 240, 242, 293
BspANI GGCC 1 cut(s) 245
BspFNI CGCG 1 cut(s) 242
BspMI ACCTGC 2 cut(s) 138, 286
BspTNI GGTCTC 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 240, 246
BssNAI GTATAC 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 246
Bst1107I GTATAC 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 317
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 2 cut(s) 240, 246
BstF5I GGATG 1 cut(s) 81
BstFNI CGCG 1 cut(s) 242
BstMAI GTCTC 1 cut(s) 283
BstMWI GCNNNNNNNGC 2 cut(s) 215, 248
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 276
BstZ17I GTATAC 1 cut(s) 226
BsuRI GGCC 1 cut(s) 245
BtgI CCRYGG 2 cut(s) 240, 246
BtsCI GGATG 1 cut(s) 81
BveI ACCTGC 2 cut(s) 138, 286
Cfr42I CCGCGG 1 cut(s) 243
CviAII CATG 3 cut(s) 119, 235, 247
CviJI RGCY 4 cut(s) 55, 112, 245, 251
CviKI_1 RGCY 4 cut(s) 55, 112, 245, 251
DdeI CTNAG 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 246
FaeI CATG 3 cut(s) 122, 238, 250
FaiI YATR 5 cut(s) 120, 226, 236, 248, 313
FatI CATG 3 cut(s) 118, 234, 246
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 243, 290, 293
FokI GGATG 1 cut(s) 68
Fsp4HI GCNGC 3 cut(s) 243, 290, 293
FspBI CTAG 1 cut(s) 86
GluI GCNGC 3 cut(s) 243, 290, 293
HaeIII GGCC 1 cut(s) 245
Hin1II CATG 3 cut(s) 122, 238, 250
HinfI GANTC 3 cut(s) 5, 130, 137
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 226
Hpy188III TCNNGA 3 cut(s) 32, 134, 168
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 248
HpyF3I CTNAG 1 cut(s) 56
Hsp92II CATG 3 cut(s) 122, 238, 250
KspI CCGCGG 1 cut(s) 243
LpnPI CCDG 3 cut(s) 133, 153, 281
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 2 cut(s) 190, 227
MaeI CTAG 1 cut(s) 86
MluCI AATT 4 cut(s) 38, 161, 171, 265
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 1 cut(s) 316
MseI TTAA 2 cut(s) 126, 319
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 242
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 215, 248
NcoI CCATGG 1 cut(s) 246
NlaIII CATG 3 cut(s) 122, 238, 250
PfeI GAWTC 2 cut(s) 130, 137
PkrI GCNGC 3 cut(s) 244, 291, 294
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
RseI CAYNNNNRTG 1 cut(s) 251
SacII CCGCGG 1 cut(s) 243
SaqAI TTAA 2 cut(s) 126, 319
SatI GCNGC 3 cut(s) 243, 290, 293
SchI GAGTC 1 cut(s) 14
SetI ASST 4 cut(s) 114, 152, 265, 300
SfaNI GCATC 2 cut(s) 190, 227
Sfr303I CCGCGG 1 cut(s) 243
SgrBI CCGCGG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
Sse9I AATT 4 cut(s) 38, 161, 171, 265
SsiI CCGC 3 cut(s) 240, 242, 293
SspMI CTAG 1 cut(s) 86
StyI CCWWGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 317
TasI AATT 4 cut(s) 38, 161, 171, 265
TauI GCSGC 2 cut(s) 245, 295
TfiI GAWTC 2 cut(s) 130, 137
Tru1I TTAA 2 cut(s) 126, 319
Tru9I TTAA 2 cut(s) 126, 319
TseI GCWGC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 70
XapI RAATTY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 2 cut(s) 148, 253
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.