Prupe.2G275600_v2.0.a1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010964
Physical Location & Seq
Reverse (-)
27748709 .. 27750852
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G275600.1

Sequence Viewer

Length: 1176 bp
ATGGAAAACACATTCACACGCAGGCAGCAGCAGCAATCCAGTCCTCGCCTTCTACGAAGCAGAAGTGGAACAATTATTCCGACCATAGCCTTGCCACCAAACAACAGCCAAAGATTCACCACCCCCCGCTCAAAATCCACCACCAAATCACGAACCACCAGTAAGAATCACGAGGAAGAGAAAGTTTTAGTTCTAACGCAAAACAAGGGTCTAGAAAATGCTATTAGACAACAAGGTACTAATTTAAATGTAACTACTAGTAGTTTTGGTAAGTTGTTGCCTCGTGGGAGAACTAGTCCTAGTCCCCGCCCTAGAATATCATCGGTGCCACCTCCACGGTCGCCGTCTGCGTGGGCATTATCTCCGGGACGATATTTGCCGTGCAAGGTGGCACCGGAGTCACCAGCTGTTTCAACAAAAGCAGCGAGGGTTAAATCCAGTCATAGCGGTGGCGGTGGTGTGAGTGGGGTTTTGAAGTATTTTAGGCTGCAGAAGAAAGTGTCACCAATACAAGAGGAGGACTTCCACCGGTTTCGTCTTCTGCATAATAGGCTGCTTCAGTGGAGATTTGCCAACGCCAGAGCTGAGGCTTCCCTGCTCGTCGCAAAAAGAGTTTCACGGGCTAAAATATTTAGCGTGTGCCTTAGAACATTGAAAGTGAGAAACTTCATCCTAGAGAAGCGAATGCAAATGCAAAAGCTCAAGCATGAGATAAAGGTGTACCAGATTCTGAACCCACAAATTTTCCTACTGAATGAATGGGGGAAATTGGATAGAAAAAATCAAGAGTCTGTGAGCAGGTTGGTCCGAAAATTGTCTGGAATTTCGAATACCCTCCCTTTGGTCCATGATGCTAAGGCTGACGTGGCGTCTGTATGCGAAGCCATGACCACGGCCATGCTTGTCATGGAAGGGATTGAAGCAATGGCCACTGAGTTGCTCCCGCAGCTCGAAAAGGTACTTTACATGGTGACGGAGCTCCTAATTGCGCAGAAACAACAGAGAGACTTGGAGGAGAACATAACTACGGTTGCTGCACTTGTGGAGGAAGAGGCAAGTGTAAGAGCACATCTCATCCAACTAGAGAGTCAAGGAGCTGAGGCCAACATACCATCGACATATAAAATCCGAATAAACAATACTGATATTAACGTGGTTAATGTATATGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

392

Amino Acids

43.96

Weight (kDa)

10.31

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 990
Acc36I ACCTGC 1 cut(s) 789
AccB1I GGYRCC 2 cut(s) 325, 391
AccBSI CCGCTC 1 cut(s) 129
AciI CCGC 5 cut(s) 127, 307, 447, 453, 944
AcoI YGGCCR 2 cut(s) 894, 927
AcsI RAATTY 2 cut(s) 741, 822
AcuI CTGAAG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 869
AfaI GTAC 3 cut(s) 238, 722, 960
AfiI CCNNNNNNNGG 1 cut(s) 841
AgeI ACCGGT 1 cut(s) 528
AgsI TTSAA 4 cut(s) 414, 475, 655, 920
AhdI GACNNNNNGTC 1 cut(s) 868
AhlI ACTAGT 2 cut(s) 257, 293
AjiI CACGTC 1 cut(s) 865
AloI GAACNNNNNNTCC 2 cut(s) 61, 93
AluBI AGCT 6 cut(s) 407, 584, 700, 949, 979, 1097
AluI AGCT 6 cut(s) 407, 584, 700, 949, 979, 1097
Alw21I GWGCWC 2 cut(s) 981, 1069
Alw26I GTCTC 1 cut(s) 999
AlwNI CAGNNNCTG 1 cut(s) 730
AoxI GGCC 3 cut(s) 894, 927, 1101
ApeKI GCWGC 8 cut(s) 25, 28, 31, 422, 487, 553, 946, 1034
ApoI RAATTY 2 cut(s) 741, 822
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AsiGI ACCGGT 1 cut(s) 528
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 2 cut(s) 805, 844
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 4 cut(s) 109, 393, 495, 982
AsuII TTCGAA 1 cut(s) 827
AvaII GGWCC 2 cut(s) 805, 844
BalI TGGCCA 1 cut(s) 929
BanI GGYRCC 2 cut(s) 325, 391
BanII GRGCYC 1 cut(s) 981
BauI CACGAG 2 cut(s) 170, 282
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 981, 1069
BbvCI CCTCAGC 2 cut(s) 585, 1098
BbvI GCAGC 8 cut(s) 37, 40, 43, 434, 474, 540, 958, 1021
BccI CCATC 1 cut(s) 1120
BceAI ACGGC 3 cut(s) 328, 364, 909
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 999
BcuI ACTAGT 2 cut(s) 257, 293
BfaI CTAG 8 cut(s) 212, 258, 294, 300, 312, 674, 1082, 1174
BfmI CTRYAG 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 8 cut(s) 26, 29, 32, 423, 488, 554, 947, 1035
BlsI GCNGC 8 cut(s) 27, 30, 33, 424, 489, 555, 948, 1036
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 805, 844
BmeRI GACNNNNNGTC 1 cut(s) 868
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 2 cut(s) 805, 844
BmiI GGNNCC 2 cut(s) 327, 393
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 841
BpiI GAAGAC 1 cut(s) 530
BplI GAGNNNNNCTC 2 cut(s) 1056, 1088
Bpu10I CCTNAGC 3 cut(s) 585, 855, 1098
Bpu14I TTCGAA 1 cut(s) 827
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 335, 891
BsaWI WCCGGW 2 cut(s) 394, 528
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 841
Bse118I RCCGGY 1 cut(s) 528
Bse1I ACTGG 3 cut(s) 39, 159, 438
Bse3DI GCAATG 1 cut(s) 930
BseDI CCNNGG 2 cut(s) 335, 891
BseGI GGATG 2 cut(s) 669, 1074
BseLI CCNNNNNNNGG 1 cut(s) 841
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 3 cut(s) 576, 924, 1089
BseNI ACTGG 3 cut(s) 39, 159, 438
BseRI GAGGAG 2 cut(s) 530, 1028
BseXI GCAGC 8 cut(s) 37, 40, 43, 434, 474, 540, 958, 1021
BsgI GTGCAG 1 cut(s) 1020
Bsh1285I CGRYCG 1 cut(s) 341
BshFI GGCC 3 cut(s) 896, 929, 1103
BshNI GGYRCC 2 cut(s) 325, 391
BshTI ACCGGT 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 341
BsiHKAI GWGCWC 2 cut(s) 981, 1069
BsiSI CCGG 3 cut(s) 365, 395, 529
BslFI GGGAC 2 cut(s) 288, 381
BslI CCNNNNNNNGG 1 cut(s) 841
BsmAI GTCTC 1 cut(s) 999
BsmFI GGGAC 2 cut(s) 288, 381
BsmI GAATGC 1 cut(s) 690
BsnI GGCC 3 cut(s) 896, 929, 1103
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 2 cut(s) 981, 1069
BspACI CCGC 5 cut(s) 127, 307, 447, 453, 944
BspANI GGCC 3 cut(s) 896, 929, 1103
BspCNI CTCAG 3 cut(s) 577, 925, 1090
BspLI GGNNCC 2 cut(s) 327, 393
BspMAI CTGCAG 1 cut(s) 492
BspMI ACCTGC 1 cut(s) 789
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 2 cut(s) 325, 391
BsrBI CCGCTC 1 cut(s) 129
BsrDI GCAATG 1 cut(s) 930
BsrFI RCCGGY 1 cut(s) 528
BsrI ACTGG 3 cut(s) 39, 159, 438
BssAI RCCGGY 1 cut(s) 528
BssECI CCNNGG 2 cut(s) 335, 891
BssNI GRCGYC 1 cut(s) 869
BssSI CACGAG 2 cut(s) 170, 282
Bst2BI CACGAG 2 cut(s) 170, 282
Bst4CI ACNGT 2 cut(s) 339, 1030
Bst6I CTCTTC 2 cut(s) 171, 1044
BstACI GRCGYC 1 cut(s) 869
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 5 cut(s) 585, 644, 855, 933, 1098
BstDSI CCRYGG 2 cut(s) 335, 891
BstF5I GGATG 2 cut(s) 669, 1074
BstHHI GCGC 1 cut(s) 991
BstMAI GTCTC 1 cut(s) 999
BstMCI CGRYCG 1 cut(s) 341
BstMWI GCNNNNNNNGC 4 cut(s) 31, 550, 866, 946
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 488
BstV1I GCAGC 8 cut(s) 37, 40, 43, 434, 474, 540, 958, 1021
BstV2I GAAGAC 1 cut(s) 530
BsuRI GGCC 3 cut(s) 896, 929, 1103
BtgI CCRYGG 2 cut(s) 335, 891
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 2 cut(s) 669, 1074
BtsIMutI CAGTG 2 cut(s) 566, 930
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 730
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 528
Cfr13I GGNCC 2 cut(s) 805, 844
CseI GACGC 1 cut(s) 858
Csp6I GTAC 3 cut(s) 237, 721, 959
CspAI ACCGGT 1 cut(s) 528
CviAII CATG 6 cut(s) 707, 848, 886, 898, 907, 967
CviQI GTAC 3 cut(s) 237, 721, 959
DdeI CTNAG 5 cut(s) 585, 644, 855, 933, 1098
DraI TTTAAA 1 cut(s) 246
DriI GACNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 2 cut(s) 894, 927
Eam1104I CTCTTC 2 cut(s) 171, 1044
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 2 cut(s) 171, 1044
Ecl136II GAGCTC 1 cut(s) 979
Eco24I GRGCYC 1 cut(s) 981
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 2 cut(s) 805, 844
Eco53kI GAGCTC 1 cut(s) 979
Eco57I CTGAAG 1 cut(s) 542
EcoICRI GAGCTC 1 cut(s) 979
EcoRV GATATC 1 cut(s) 1171
EcoT38I GRGCYC 1 cut(s) 981
FaeI CATG 6 cut(s) 710, 851, 889, 901, 910, 970
FaqI GGGAC 2 cut(s) 288, 381
FatI CATG 6 cut(s) 706, 847, 885, 897, 906, 966
FauI CCCGC 3 cut(s) 134, 314, 951
Fnu4HI GCNGC 8 cut(s) 26, 29, 32, 423, 488, 554, 947, 1035
FokI GGATG 2 cut(s) 656, 1061
FriOI GRGCYC 1 cut(s) 981
Fsp4HI GCNGC 8 cut(s) 26, 29, 32, 423, 488, 554, 947, 1035
FspBI CTAG 8 cut(s) 212, 258, 294, 300, 312, 674, 1082, 1174
FspI TGCGCA 1 cut(s) 990
GlaI GCGC 1 cut(s) 990
GluI GCNGC 8 cut(s) 26, 29, 32, 423, 488, 554, 947, 1035
HaeIII GGCC 3 cut(s) 896, 929, 1103
HapII CCGG 3 cut(s) 365, 395, 529
HgaI GACGC 1 cut(s) 858
HhaI GCGC 1 cut(s) 991
Hin1I GRCGYC 1 cut(s) 869
Hin1II CATG 6 cut(s) 710, 851, 889, 901, 910, 970
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HinfI GANTC 6 cut(s) 114, 166, 398, 727, 788, 1087
HpaII CCGG 3 cut(s) 365, 395, 529
HphI GGTGA 4 cut(s) 109, 393, 495, 982
Hpy166II GTNNAC 1 cut(s) 721
Hpy188I TCNGA 4 cut(s) 81, 732, 809, 1130
Hpy188III TCNNGA 5 cut(s) 150, 170, 212, 785, 819
Hpy8I GTNNAC 1 cut(s) 721
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 2 cut(s) 59, 905
HpyCH4III ACNGT 2 cut(s) 339, 1030
HpyCH4IV ACGT 2 cut(s) 864, 1152
HpyCH4V TGCA 6 cut(s) 384, 490, 544, 688, 694, 1037
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 550, 866, 946
HpyF3I CTNAG 5 cut(s) 585, 644, 855, 933, 1098
HpySE526I ACGT 2 cut(s) 864, 1152
Hsp92I GRCGYC 1 cut(s) 869
Hsp92II CATG 6 cut(s) 710, 851, 889, 901, 910, 970
HspAI GCGC 1 cut(s) 989
LmnI GCTCC 4 cut(s) 945, 976, 984, 1094
Lsp1109I GCAGC 8 cut(s) 37, 40, 43, 434, 474, 540, 958, 1021
LweI GCATC 1 cut(s) 841
MaeI CTAG 8 cut(s) 212, 258, 294, 300, 312, 674, 1082, 1174
MaeII ACGT 2 cut(s) 864, 1152
MaeIII GTNAC 4 cut(s) 250, 399, 501, 970
MbiI CCGCTC 1 cut(s) 129
MboII GAAGA 4 cut(s) 188, 505, 530, 1061
MhlI GDGCHC 2 cut(s) 981, 1069
MlsI TGGCCA 1 cut(s) 929
MluCI AATT 7 cut(s) 72, 241, 741, 767, 812, 822, 984
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 3 cut(s) 407, 797, 1096
MmeI TCCRAC 2 cut(s) 104, 1102
Mox20I TGGCCA 1 cut(s) 929
MscI TGGCCA 1 cut(s) 929
MseI TTAA 4 cut(s) 245, 432, 1149, 1158
MslI CAYNNNNRTG 2 cut(s) 447, 896
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 3 cut(s) 365, 395, 529
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 690
MwoI GCNNNNNNNGC 4 cut(s) 31, 550, 866, 946
NciI CCSGG 1 cut(s) 366
NlaIII CATG 6 cut(s) 710, 851, 889, 901, 910, 970
NlaIV GGNNCC 2 cut(s) 327, 393
NmuCI GTSAC 3 cut(s) 399, 501, 970
NsbI TGCGCA 1 cut(s) 990
NspV TTCGAA 1 cut(s) 827
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 347
PctI GAATGC 1 cut(s) 690
PfeI GAWTC 3 cut(s) 114, 166, 727
PfoI TCCNGGA 1 cut(s) 364
PinAI ACCGGT 1 cut(s) 528
PkrI GCNGC 8 cut(s) 27, 30, 33, 424, 489, 555, 948, 1036
PleI GAGTC 3 cut(s) 406, 796, 1095
PpsI GAGTC 3 cut(s) 406, 796, 1095
Psp124BI GAGCTC 1 cut(s) 981
PspN4I GGNNCC 2 cut(s) 327, 393
PspPI GGNCC 2 cut(s) 805, 844
PstI CTGCAG 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 730
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 238, 722, 960
RsaNI GTAC 3 cut(s) 237, 721, 959
RseI CAYNNNNRTG 2 cut(s) 447, 896
SacI GAGCTC 1 cut(s) 981
SaqAI TTAA 4 cut(s) 245, 432, 1149, 1158
SatI GCNGC 8 cut(s) 26, 29, 32, 423, 488, 554, 947, 1035
Sau96I GGNCC 2 cut(s) 805, 844
SchI GAGTC 3 cut(s) 407, 797, 1096
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 2 cut(s) 981, 1069
SfaNI GCATC 1 cut(s) 841
SfcI CTRYAG 1 cut(s) 488
SfuI TTCGAA 1 cut(s) 827
SinI GGWCC 2 cut(s) 805, 844
SmiI ATTTAAAT 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 447, 896
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SpeI ACTAGT 2 cut(s) 257, 293
Sse9I AATT 7 cut(s) 72, 241, 741, 767, 812, 822, 984
SsiI CCGC 5 cut(s) 127, 307, 447, 453, 944
SspI AATATT 1 cut(s) 630
SspMI CTAG 8 cut(s) 212, 258, 294, 300, 312, 674, 1082, 1174
SstI GAGCTC 1 cut(s) 981
StyD4I CCNGG 1 cut(s) 364
SwaI ATTTAAAT 1 cut(s) 246
TaaI ACNGT 2 cut(s) 339, 1030
TaiI ACGT 2 cut(s) 867, 1155
TaqI TCGA 3 cut(s) 827, 951, 1115
TasI AATT 7 cut(s) 72, 241, 741, 767, 812, 822, 984
TfiI GAWTC 3 cut(s) 114, 166, 727
Tru1I TTAA 4 cut(s) 245, 432, 1149, 1158
Tru9I TTAA 4 cut(s) 245, 432, 1149, 1158
TscAI CASTG 2 cut(s) 566, 937
TseFI GTSAC 3 cut(s) 399, 501, 970
TseI GCWGC 8 cut(s) 25, 28, 31, 422, 487, 553, 946, 1034
Tsp45I GTSAC 3 cut(s) 399, 501, 970
TspDTI ATGAA 2 cut(s) 658, 771
TspGWI ACGGA 1 cut(s) 989
TspRI CASTG 2 cut(s) 566, 937
VpaK11BI GGWCC 2 cut(s) 805, 844
XapI RAATTY 2 cut(s) 741, 822
XbaI TCTAGA 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XspI CTAG 8 cut(s) 212, 258, 294, 300, 312, 674, 1082, 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.