MD07G1252100.v1.1

QWRF motif-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010964
Physical Location & Seq
Reverse (-)
32000247 .. 32002679
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1252100.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGAAAACACATACATGTCACGCAGGCAGCAGCAGCAATCCAGCCCTCGACTTCTCAGAAGCAGAAGTGGAACTTCTTCGACCCCGACCCTAACCCTACCGCAAAACGTTTCACGTTCATGCACTACCACGCCAAACAACAGCCAAAGATTCACCAGCCCCAAGCGCTCGAAATCCACCGTGAAATCAAGAACCAATAAGAATCACGAGGAAGAGAAAGTACATGCACTCAAGCAAAAGAAGGGTGGCCAAGAAGATATCATTGGACAACAAGGTACTAATAATTTAGGTATAAATACGAGTTTTGCTAAGTTGCAGCGAGGGAGTACTAGTCCTAGTCCAAGTCCACGCCCTAGAAAATCAGTGTCCATGCCGTCTCCGCCGTCTCCTTCAGCATGGGCTTTGTCCCCGGGACGGTCTTTGCCGCGCAAGGTGGCACCGGATCCCCCAGCGGCGGCGCCTTCGACAAGGGCAGCCAGGGTTAAGTCTAGTGGTGGCGGGGTTGGTGGGGTTTTAAAGTATTTTAGGCTGCAGAAGAAAGTGCCGCCGGTACAAGAGGAGGACTTCCACCGGTTTCCCCTTTTGCATAATAGGCTGCTGCAGTGGAGGTTTGCAAACGCCAGAGCTGAGGCTGCCAAGCTTGATGCACAGAGAATTTCACAGGATAAATTATTTAGTGTGTGGCTTAGAACATTGAAAGTGAGAAAGTTCATCCTAGACAAAAGAATACAAGTGCAAAAGCTCAAGCATGAGATTAAGGTGTACCAGATTTTGAACCCACAAATTTTCCTACTGAATGAATGGGGAAAATTGGATAAAAAAAATCAAGAATCCATCAGCAGGTTGGTGAGAAAATTGTCAGGAATGTCAAACACAATCCCTTTGGTCAATGATGCTAAGACAGATGTGGCATCTGTATATGAAGCCATGACCTCGGCCATGGCTGCGATGGAAGATAATTTAGGAATGGTCTCTAAACTGCTGCCGGAGCTGGAAAAAGTGCTTTACATGGTTACGGAGCTTTTAATCTCACAGAAACAACAGAGAGACTTGGAGAGTAATTTAGCCTTGGTTTCTGCACTTGCGGAGGAGGAGGCAAGTCTAAGAGCACATCTCATCCAATTGGAATCAATGTACTTCTTGCCCCCAACAAATTCTTATAAATATGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

392

Amino Acids

43.93

Weight (kDa)

10.25

Isoelectric Point (pI)

65.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 33 - 349 3.1e-61 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
Acc36I ACCTGC 1 cut(s) 831
AccB1I GGYRCC 2 cut(s) 436, 457
AccII CGCG 1 cut(s) 427
AciI CCGC 8 cut(s) 101, 380, 425, 452, 455, 498, 545, 1085
AclI AACGTT 1 cut(s) 108
AclWI GGATC 2 cut(s) 437, 450
AcoI YGGCCR 2 cut(s) 247, 936
AcsI RAATTY 3 cut(s) 654, 783, 1153
AcuI CTGAAG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 458
AfaI GTAC 6 cut(s) 222, 277, 328, 552, 764, 1136
AfeI AGCGCT 1 cut(s) 167
AfiI CCNNNNNNNGG 3 cut(s) 414, 454, 840
AflIII ACRYGT 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 2 cut(s) 697, 775
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 1 cut(s) 476
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 5 cut(s) 626, 640, 742, 991, 1021
AluI AGCT 5 cut(s) 626, 640, 742, 991, 1021
Alw21I GWGCWC 1 cut(s) 1111
Alw26I GTCTC 4 cut(s) 381, 390, 976, 1041
AlwI GGATC 2 cut(s) 437, 450
Ama87I CYCGRG 1 cut(s) 409
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 2 cut(s) 247, 936
ApoI RAATTY 3 cut(s) 654, 783, 1153
AsiGI ACCGGT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 76
AspLEI GCGC 3 cut(s) 168, 429, 460
AsuC2I CCSGG 2 cut(s) 410, 411
AsuHPI GGTGA 2 cut(s) 145, 859
AvaI CYCGRG 1 cut(s) 409
BalI TGGCCA 1 cut(s) 249
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 2 cut(s) 436, 457
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 1111
BbvCI CCTCAGC 1 cut(s) 627
BccI CCATC 2 cut(s) 842, 943
BceAI ACGGC 2 cut(s) 358, 367
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 1 cut(s) 1168
BcnI CCSGG 2 cut(s) 410, 411
BcoDI GTCTC 4 cut(s) 381, 390, 976, 1041
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 5 cut(s) 330, 336, 354, 489, 716
BfmI CTRYAG 2 cut(s) 530, 599
BfoI RGCGCY 2 cut(s) 169, 461
BfuAI ACCTGC 1 cut(s) 831
BmcAI AGTACT 1 cut(s) 328
Bme1390I CCNGG 3 cut(s) 410, 411, 478
BmeT110I CYCGRG 1 cut(s) 409
BmiI GGNNCC 3 cut(s) 438, 444, 459
BmrFI CCNGG 3 cut(s) 410, 411, 478
BmsI GCATC 3 cut(s) 634, 883, 920
BplI GAGNNNNNCTC 2 cut(s) 1098, 1130
Bpu10I CCTNAGC 1 cut(s) 627
BpuEI CTTGAG 2 cut(s) 215, 728
BpuMI CCSGG 2 cut(s) 410, 411
BsaHI GRCGYC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 976
BsaJI CCNNGG 6 cut(s) 408, 409, 477, 933, 939, 1068
BsaWI WCCGGW 2 cut(s) 439, 570
BsaXI ACNNNNNCTCC 2 cut(s) 316, 346
Bsc4I CCNNNNNNNGG 3 cut(s) 414, 454, 840
Bse118I RCCGGY 2 cut(s) 547, 570
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 6 cut(s) 408, 409, 477, 933, 939, 1068
BseGI GGATG 2 cut(s) 711, 1116
BseLI CCNNNNNNNGG 3 cut(s) 414, 454, 840
BseMII CTCAG 2 cut(s) 70, 618
BseRI GAGGAG 3 cut(s) 572, 1103, 1106
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 1 cut(s) 1062
Bsh1236I CGCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 249, 938
BshNI GGYRCC 2 cut(s) 436, 457
BshTI ACCGGT 1 cut(s) 570
BsiHKAI GWGCWC 1 cut(s) 1111
BsiHKCI CYCGRG 1 cut(s) 409
BsiSI CCGG 5 cut(s) 410, 440, 548, 571, 986
BslFI GGGAC 2 cut(s) 391, 426
BslI CCNNNNNNNGG 3 cut(s) 414, 454, 840
BsmAI GTCTC 4 cut(s) 381, 390, 976, 1041
BsmBI CGTCTC 2 cut(s) 381, 390
BsmFI GGGAC 2 cut(s) 391, 426
BsnI GGCC 2 cut(s) 249, 938
Bso31I GGTCTC 1 cut(s) 976
BsoBI CYCGRG 1 cut(s) 409
Bsp1286I GDGCHC 1 cut(s) 1111
Bsp143I GATC 2 cut(s) 442, 1168
Bsp19I CCATGG 1 cut(s) 939
BspACI CCGC 8 cut(s) 101, 380, 425, 452, 455, 498, 545, 1085
BspANI GGCC 2 cut(s) 249, 938
BspCNI CTCAG 2 cut(s) 69, 619
BspFNI CGCG 1 cut(s) 427
BspLI GGNNCC 3 cut(s) 438, 444, 459
BspMAI CTGCAG 2 cut(s) 534, 603
BspMI ACCTGC 1 cut(s) 831
BspPI GGATC 2 cut(s) 437, 450
BspT107I GGYRCC 2 cut(s) 436, 457
BspTNI GGTCTC 1 cut(s) 976
BsrFI RCCGGY 2 cut(s) 547, 570
BssAI RCCGGY 2 cut(s) 547, 570
BssECI CCNNGG 6 cut(s) 408, 409, 477, 933, 939, 1068
BssMI GATC 2 cut(s) 442, 1168
BssNI GRCGYC 1 cut(s) 458
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 939, 1068
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 181, 417
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 6 cut(s) 56, 309, 627, 686, 897, 1103
BstDSI CCRYGG 1 cut(s) 939
BstF5I GGATG 2 cut(s) 711, 1116
BstFNI CGCG 1 cut(s) 427
BstH2I RGCGCY 2 cut(s) 169, 461
BstHHI GCGC 3 cut(s) 168, 429, 460
BstKTI GATC 2 cut(s) 445, 1171
BstMAI GTCTC 4 cut(s) 381, 390, 976, 1041
BstMBI GATC 2 cut(s) 442, 1168
BstMWI GCNNNNNNNGC 7 cut(s) 34, 165, 379, 592, 632, 944, 988
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 2 cut(s) 19, 227
BstSCI CCNGG 3 cut(s) 408, 409, 476
BstSFI CTRYAG 2 cut(s) 530, 599
BstUI CGCG 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 2 cut(s) 249, 938
BtgI CCRYGG 1 cut(s) 939
BtgZI GCGATG 1 cut(s) 962
BtsCI GGATG 2 cut(s) 711, 1116
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 2 cut(s) 369, 608
BveI ACCTGC 1 cut(s) 831
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 3 cut(s) 168, 429, 460
Cfr10I RCCGGY 2 cut(s) 547, 570
Cfr9I CCCGGG 1 cut(s) 409
Csp6I GTAC 6 cut(s) 221, 276, 327, 551, 763, 1135
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 9 cut(s) 16, 120, 224, 370, 396, 749, 928, 940, 1009
CviQI GTAC 6 cut(s) 221, 276, 327, 551, 763, 1135
DdeI CTNAG 6 cut(s) 56, 309, 627, 686, 897, 1103
DinI GGCGCC 1 cut(s) 459
DpnI GATC 2 cut(s) 444, 1170
DpnII GATC 2 cut(s) 442, 1168
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 2 cut(s) 247, 936
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EciI GGCGGA 1 cut(s) 369
Eco130I CCWWGG 2 cut(s) 939, 1068
Eco31I GGTCTC 1 cut(s) 976
Eco32I GATATC 1 cut(s) 259
Eco47III AGCGCT 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 2 cut(s) 939, 1068
EgeI GGCGCC 1 cut(s) 459
EheI GGCGCC 1 cut(s) 459
ErhI CCWWGG 2 cut(s) 939, 1068
Esp3I CGTCTC 2 cut(s) 381, 390
FaeI CATG 9 cut(s) 19, 123, 227, 373, 399, 752, 931, 943, 1012
FalI AAGNNNNNCTT 2 cut(s) 58, 90
FaqI GGGAC 2 cut(s) 391, 426
FatI CATG 9 cut(s) 15, 119, 223, 369, 395, 748, 927, 939, 1008
FauI CCCGC 1 cut(s) 491
FbaI TGATCA 1 cut(s) 1168
FokI GGATG 2 cut(s) 698, 1103
FspBI CTAG 5 cut(s) 330, 336, 354, 489, 716
GlaI GCGC 3 cut(s) 167, 428, 459
GsaI CCCAGC 1 cut(s) 452
HaeII RGCGCY 2 cut(s) 169, 461
HaeIII GGCC 2 cut(s) 249, 938
HapII CCGG 5 cut(s) 410, 440, 548, 571, 986
HhaI GCGC 3 cut(s) 168, 429, 460
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 9 cut(s) 19, 123, 227, 373, 399, 752, 931, 943, 1012
Hin6I GCGC 3 cut(s) 166, 427, 458
HinP1I GCGC 3 cut(s) 166, 427, 458
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 4 cut(s) 150, 202, 830, 1127
HpaII CCGG 5 cut(s) 410, 440, 548, 571, 986
HphI GGTGA 2 cut(s) 145, 859
Hpy166II GTNNAC 2 cut(s) 347, 763
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 4 cut(s) 189, 206, 827, 861
Hpy8I GTNNAC 2 cut(s) 347, 763
HpyAV CCTTC 3 cut(s) 235, 399, 471
HpyCH4III ACNGT 2 cut(s) 181, 417
HpyCH4IV ACGT 2 cut(s) 108, 115
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 165, 379, 592, 632, 944, 988
HpyF3I CTNAG 6 cut(s) 56, 309, 627, 686, 897, 1103
HpySE526I ACGT 2 cut(s) 108, 115
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 9 cut(s) 19, 123, 227, 373, 399, 752, 931, 943, 1012
HspAI GCGC 3 cut(s) 166, 427, 458
KasI GGCGCC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 1168
Kzo9I GATC 2 cut(s) 442, 1168
LmnI GCTCC 2 cut(s) 988, 1018
LweI GCATC 3 cut(s) 634, 883, 920
MaeI CTAG 5 cut(s) 330, 336, 354, 489, 716
MaeII ACGT 2 cut(s) 108, 115
MaeIII GTNAC 2 cut(s) 18, 1012
MalI GATC 2 cut(s) 444, 1170
MboI GATC 2 cut(s) 442, 1168
MboII GAAGA 5 cut(s) 69, 224, 266, 547, 965
MfeI CAATTG 1 cut(s) 1121
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 1111
MlsI TGGCCA 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 249
Mly113I GGCGCC 1 cut(s) 458
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 249
MseI TTAA 4 cut(s) 483, 515, 756, 1025
MslI CAYNNNNRTG 2 cut(s) 14, 118
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 5 cut(s) 410, 440, 548, 571, 986
MspR9I CCNGG 3 cut(s) 410, 411, 478
MunI CAATTG 1 cut(s) 1121
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 427
MwoI GCNNNNNNNGC 7 cut(s) 34, 165, 379, 592, 632, 944, 988
NarI GGCGCC 1 cut(s) 458
NciI CCSGG 2 cut(s) 410, 411
NcoI CCATGG 1 cut(s) 939
NdeII GATC 2 cut(s) 442, 1168
NlaIII CATG 9 cut(s) 19, 123, 227, 373, 399, 752, 931, 943, 1012
NlaIV GGNNCC 3 cut(s) 438, 444, 459
NmeAIII GCCGAG 1 cut(s) 914
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 2 cut(s) 19, 227
PciI ACATGT 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 76
PfeI GAWTC 4 cut(s) 150, 202, 830, 1127
PinAI ACCGGT 1 cut(s) 570
PluTI GGCGCC 1 cut(s) 461
PscI ACATGT 1 cut(s) 15
PsiI TTATAA 1 cut(s) 1161
Psp1406I AACGTT 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 3 cut(s) 438, 444, 459
PstI CTGCAG 2 cut(s) 534, 603
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 6 cut(s) 222, 277, 328, 552, 764, 1136
RsaNI GTAC 6 cut(s) 221, 276, 327, 551, 763, 1135
RseI CAYNNNNRTG 2 cut(s) 14, 118
SaqAI TTAA 4 cut(s) 483, 515, 756, 1025
Sau3AI GATC 2 cut(s) 442, 1168
ScaI AGTACT 1 cut(s) 328
ScrFI CCNGG 3 cut(s) 410, 411, 478
SduI GDGCHC 1 cut(s) 1111
SfaNI GCATC 3 cut(s) 634, 883, 920
SfcI CTRYAG 2 cut(s) 530, 599
SfoI GGCGCC 1 cut(s) 459
SmaI CCCGGG 1 cut(s) 411
SmiMI CAYNNNNRTG 2 cut(s) 14, 118
SmlI CTYRAG 2 cut(s) 230, 743
SmoI CTYRAG 2 cut(s) 230, 743
SpeI ACTAGT 1 cut(s) 329
SsiI CCGC 8 cut(s) 101, 380, 425, 452, 455, 498, 545, 1085
SspDI GGCGCC 1 cut(s) 457
SspMI CTAG 5 cut(s) 330, 336, 354, 489, 716
StyD4I CCNGG 3 cut(s) 408, 409, 476
StyI CCWWGG 2 cut(s) 939, 1068
TaaI ACNGT 2 cut(s) 181, 417
TaiI ACGT 2 cut(s) 111, 118
TaqI TCGA 4 cut(s) 49, 80, 170, 464
TatI WGTACW 3 cut(s) 220, 326, 1134
TauI GCSGC 4 cut(s) 427, 455, 458, 547
TfiI GAWTC 4 cut(s) 150, 202, 830, 1127
Tru1I TTAA 4 cut(s) 483, 515, 756, 1025
Tru9I TTAA 4 cut(s) 483, 515, 756, 1025
TscAI CASTG 2 cut(s) 369, 608
TseFI GTSAC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 108, 700, 813, 936
TspGWI ACGGA 1 cut(s) 1031
TspMI CCCGGG 1 cut(s) 409
TspRI CASTG 2 cut(s) 369, 608
XapI RAATTY 3 cut(s) 654, 783, 1153
XceI RCATGY 2 cut(s) 19, 227
XcmI CCANNNNNNNNNTGG 2 cut(s) 841, 946
XmaI CCCGGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 76
XspI CTAG 5 cut(s) 330, 336, 354, 489, 716
ZrmI AGTACT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.